Carg19593 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19593
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	diacylglycerol O-acyltransferase 3, cytosolic-like
Location	Carg_Chr14: 11586422 .. 11588166 (+)
RNA-Seq Expression	Carg19593
Synteny	Carg19593

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTCCACGTGAGTCGACACGAGGTCCTCCATCTTTCGAATCAAAATCCGCCACGTCAGCAAAACTCGATCCTTCCGTAATTTGGGTTAGTTTCAAAATTTGATTCGTCGCTTCTTTCCTAGTTGAAATCTTCAATCCATCCTCCTCGAATCCATGGAGGTCTCCGGCGCCGTTACCCGGCGGCTCTCTAGCCTCTTCCGCTGCGAGATTGCTCAATCCGTTGATGTCGCCAGCGCCGATGCACGGCGGGCTCCCTGTAGCCTGTCCGTTTCCGGTGGCTCTGTTAGTACTGGAACTCGCGTTATTGGCCTCGTCGGTCGTCGGAATATAGATTCTTCTAGGTTTTCTGATAATGGTCATTTGCGATATTATGTTGGACCGAATTGCCAGGGGAGGAGGTTGAAGAAGGAGAAGGAAGCGGTGAAGAAGAAATTGAAATTGTTGAAAGGATTGTCCGGTTATTCATCTACGGATCACGAATCTGGCTTAGTGTTTCGATTTGATGATTGCTTGATCGGAGACTTTCAAGCGGATCGCTTCTCTGTAAGTTGAACAATTCTGTAAAATCGCTTCAAGCATTTTTAAAATCTCTTGACCTAAATCCGGTTTGATCGCGCTACTTGATCTGCACCAGCTGAATAATTGAGATGTTTTCTTCTCTTTATCCGAATTAAGTAATATAAATTATCCATGTTGAATCGGAGTTGAGCTTGAGATTTCTTTGCATATACTTGCGATCCAAATCTCTTGTTTCTGCTCGATCGCACTAGAACTCAATCTTCATGAATGATTTCTTGTTGTATTTGTTTTGGCTGAATAGATTGGTTGATAACATCCATTGATCGCATTGTTGTTCCCTTAATTGCAGATCGAGGAAACAAGAGAGGCATTGGTAAAACAGCTTCAACAACTAAAATCTGAGGAAAAGGACCAGAAAAGAAGGAGGAAGTTAGAGAAGGCCAAGCTTAAAGCAACTCGAGCAAAAACAATTCACGAGTCATCATCATCTTCATCCTCTGAATCCAGTGACAACGAAGGCTACATGACCAACACAAGCTACCAAATAAGGAAGGCACTTTCACAGCCATTCCCAGATCAATGGAAGACAAATGGCATCAAAGAATCAACACTGCCTCCTCCTTTGCAATCCCAACTGCTCAATTCCGACACAAAAAACATCGGCGTCGCACGATCGGTATCTGTAGGGAGGGTTGAAGTGTGTATGGGAAACAAGTGCAAGAAGGCAGGGGCAGCAGCATTGATGGAGGAATTTGAGAGGGTGATGGGAGCTGATGGTGCTGTTTGTGGGTGTAAATGCATGGGGAAATGCAGAGATGGACCAAATGTTAGAGTCTCTGGTGCGTTGGCCGGACAAAGCTGCTCTGCAAATCCTCTGTGCATTGGAGTTGGAGTGGAGGATGTTGGCAGAATTGTAGCTGATCATCTTGGACAACAAGGGGAGCTGAAACAATCAAGGTAACACCTGCAACTGATGATACTCATCGGAGAAGCTAAATGATCTTCCCCTTTTGAGTTATACTGAGTTGGGTTGGGATAACCAACTTTTTGGACCCAAACCAACTATTAAAATTTCAAATTTGTCAATCCAATCCATGAAAGGAAGCTATATGGTGAATCCCAACTCAACTCGAGTTCTGTTGATTCGGTTATTAGTTTACACTCTTGTTAAAAAAATATTGAATTTACACTATTTCAATACTAATGACTTATATTCAAGTATTAAGA

mRNA sequence

GGTCCACGTGAGTCGACACGAGGTCCTCCATCTTTCGAATCAAAATCCGCCACGTCAGCAAAACTCGATCCTTCCGTAATTTGGGTTAGTTTCAAAATTTGATTCGTCGCTTCTTTCCTAGTTGAAATCTTCAATCCATCCTCCTCGAATCCATGGAGGTCTCCGGCGCCGTTACCCGGCGGCTCTCTAGCCTCTTCCGCTGCGAGATTGCTCAATCCGTTGATGTCGCCAGCGCCGATGCACGGCGGGCTCCCTGTAGCCTGTCCGTTTCCGGTGGCTCTGTTAGTACTGGAACTCGCGTTATTGGCCTCGTCGGTCGTCGGAATATAGATTCTTCTAGGTTTTCTGATAATGGTCATTTGCGATATTATGTTGGACCGAATTGCCAGGGGAGGAGGTTGAAGAAGGAGAAGGAAGCGGTGAAGAAGAAATTGAAATTGTTGAAAGGATTGTCCGGTTATTCATCTACGGATCACGAATCTGGCTTAGTGTTTCGATTTGATGATTGCTTGATCGGAGACTTTCAAGCGGATCGCTTCTCTATCGAGGAAACAAGAGAGGCATTGGTAAAACAGCTTCAACAACTAAAATCTGAGGAAAAGGACCAGAAAAGAAGGAGGAAGTTAGAGAAGGCCAAGCTTAAAGCAACTCGAGCAAAAACAATTCACGAGTCATCATCATCTTCATCCTCTGAATCCAGTGACAACGAAGGCTACATGACCAACACAAGCTACCAAATAAGGAAGGCACTTTCACAGCCATTCCCAGATCAATGGAAGACAAATGGCATCAAAGAATCAACACTGCCTCCTCCTTTGCAATCCCAACTGCTCAATTCCGACACAAAAAACATCGGCGTCGCACGATCGGTATCTGTAGGGAGGGTTGAAGTGTGTATGGGAAACAAGTGCAAGAAGGCAGGGGCAGCAGCATTGATGGAGGAATTTGAGAGGGTGATGGGAGCTGATGGTGCTGTTTGTGGGTGTAAATGCATGGGGAAATGCAGAGATGGACCAAATGTTAGAGTCTCTGGTGCGTTGGCCGGACAAAGCTGCTCTGCAAATCCTCTGTGCATTGGAGTTGGAGTGGAGGATGTTGGCAGAATTGTAGCTGATCATCTTGGACAACAAGGGGAGCTGAAACAATCAAGGTAACACCTGCAACTGATGATACTCATCGGAGAAGCTAAATGATCTTCCCCTTTTGAGTTATACTGAGTTGGGTTGGGATAACCAACTTTTTGGACCCAAACCAACTATTAAAATTTCAAATTTGTCAATCCAATCCATGAAAGGAAGCTATATGGTGAATCCCAACTCAACTCGAGTTCTGTTGATTCGGTTATTAGTTTACACTCTTGTTAAAAAAATATTGAATTTACACTATTTCAATACTAATGACTTATATTCAAGTATTAAGA

Coding sequence (CDS)

ATGGAGGTCTCCGGCGCCGTTACCCGGCGGCTCTCTAGCCTCTTCCGCTGCGAGATTGCTCAATCCGTTGATGTCGCCAGCGCCGATGCACGGCGGGCTCCCTGTAGCCTGTCCGTTTCCGGTGGCTCTGTTAGTACTGGAACTCGCGTTATTGGCCTCGTCGGTCGTCGGAATATAGATTCTTCTAGGTTTTCTGATAATGGTCATTTGCGATATTATGTTGGACCGAATTGCCAGGGGAGGAGGTTGAAGAAGGAGAAGGAAGCGGTGAAGAAGAAATTGAAATTGTTGAAAGGATTGTCCGGTTATTCATCTACGGATCACGAATCTGGCTTAGTGTTTCGATTTGATGATTGCTTGATCGGAGACTTTCAAGCGGATCGCTTCTCTATCGAGGAAACAAGAGAGGCATTGGTAAAACAGCTTCAACAACTAAAATCTGAGGAAAAGGACCAGAAAAGAAGGAGGAAGTTAGAGAAGGCCAAGCTTAAAGCAACTCGAGCAAAAACAATTCACGAGTCATCATCATCTTCATCCTCTGAATCCAGTGACAACGAAGGCTACATGACCAACACAAGCTACCAAATAAGGAAGGCACTTTCACAGCCATTCCCAGATCAATGGAAGACAAATGGCATCAAAGAATCAACACTGCCTCCTCCTTTGCAATCCCAACTGCTCAATTCCGACACAAAAAACATCGGCGTCGCACGATCGGTATCTGTAGGGAGGGTTGAAGTGTGTATGGGAAACAAGTGCAAGAAGGCAGGGGCAGCAGCATTGATGGAGGAATTTGAGAGGGTGATGGGAGCTGATGGTGCTGTTTGTGGGTGTAAATGCATGGGGAAATGCAGAGATGGACCAAATGTTAGAGTCTCTGGTGCGTTGGCCGGACAAAGCTGCTCTGCAAATCCTCTGTGCATTGGAGTTGGAGTGGAGGATGTTGGCAGAATTGTAGCTGATCATCTTGGACAACAAGGGGAGCTGAAACAATCAAGGTAA

Protein sequence

MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNIDSSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCLIGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSSESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSVSVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQSCSANPLCIGVGVEDVGRIVADHLGQQGELKQSR

Homology

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BLAST of Carg19593 vs. NCBI nr
Match: KAG6582142.1 (Diacylglycerol O-acyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018541.1 Diacylglycerol O-acyltransferase 3, cytosolic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 639.0 bits (1647), Expect = 2.2e-179
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0


Query: 1   MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNID 60
           MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNID
Sbjct: 1   MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNID 60

Query: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL 120
           SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL
Sbjct: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL 120

Query: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSS 180
           IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSS
Sbjct: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSS 180

Query: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV 240
           ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV
Sbjct: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV 240

Query: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300
           SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS
Sbjct: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300

Query: 301 CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 334
           CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR
Sbjct: 301 CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 333

[Close]

BLAST of Carg19593 vs. NCBI nr
Match: XP_022955425.1 (diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita moschata])

HSP 1 Score: 635.6 bits (1638), Expect = 2.5e-178
Identity = 331/333 (99.40%), Postives = 331/333 (99.40%), Query Frame = 0


Query: 1   MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNID 60
           MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGT VIGLVGRRNID
Sbjct: 1   MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTHVIGLVGRRNID 60

Query: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL 120
           SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL
Sbjct: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL 120

Query: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSS 180
           IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATR KTIHESSSSSSS
Sbjct: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRVKTIHESSSSSSS 180

Query: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV 240
           ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV
Sbjct: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV 240

Query: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300
           SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS
Sbjct: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300

Query: 301 CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 334
           CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR
Sbjct: 301 CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 333

[Close]

BLAST of Carg19593 vs. NCBI nr
Match: XP_023527748.1 (diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 628.6 bits (1620), Expect = 3.0e-176
Identity = 327/333 (98.20%), Postives = 330/333 (99.10%), Query Frame = 0


Query: 1   MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNID 60
           MEVSGAVTRRLSSLFRCEI+QSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNID
Sbjct: 1   MEVSGAVTRRLSSLFRCEISQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNID 60

Query: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL 120
           SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSG SSTDHESGLVFRFDDCL
Sbjct: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGSSSTDHESGLVFRFDDCL 120

Query: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSS 180
           IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATR+KTIHESSSSSSS
Sbjct: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRSKTIHESSSSSSS 180

Query: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV 240
           ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGV RSV
Sbjct: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVTRSV 240

Query: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300
           SVGR+EVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS
Sbjct: 241 SVGRIEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300

Query: 301 CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 334
           CSANPLCIGVGVEDVGRIVADHLGQQG LKQSR
Sbjct: 301 CSANPLCIGVGVEDVGRIVADHLGQQGVLKQSR 333

[Close]

BLAST of Carg19593 vs. NCBI nr
Match: XP_022979861.1 (diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita maxima])

HSP 1 Score: 610.1 bits (1572), Expect = 1.1e-170
Identity = 317/333 (95.20%), Postives = 324/333 (97.30%), Query Frame = 0


Query: 1   MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNID 60
           MEVSGAVTRRLSSLFRCEI QSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRN+D
Sbjct: 1   MEVSGAVTRRLSSLFRCEIVQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNVD 60

Query: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL 120
           SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKK KLLKGLSGYSSTDHESGLVFR DDCL
Sbjct: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKFKLLKGLSGYSSTDHESGLVFRLDDCL 120

Query: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSS 180
           IGD QADRFSIEETREAL++QLQQLKSEEKDQKR+RKLEKAKLKATRAKTIH+SSSSSSS
Sbjct: 121 IGDSQADRFSIEETREALIRQLQQLKSEEKDQKRKRKLEKAKLKATRAKTIHKSSSSSSS 180

Query: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV 240
           ESSDNEGYM NT Y+IRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVA+SV
Sbjct: 181 ESSDNEGYMINTRYRIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVAQSV 240

Query: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300
           SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALA QS
Sbjct: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALARQS 300

Query: 301 CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 334
           CSANPLCIGVGV DVGRIVADHLGQQG LKQSR
Sbjct: 301 CSANPLCIGVGVGDVGRIVADHLGQQGGLKQSR 333

[Close]

BLAST of Carg19593 vs. NCBI nr
Match: XP_038900889.1 (diacylglycerol O-acyltransferase 3 [Benincasa hispida])

HSP 1 Score: 459.1 bits (1180), Expect = 3.1e-125
Identity = 250/333 (75.08%), Postives = 284/333 (85.29%), Query Frame = 0


Query: 1   MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNID 60
           MEVSGAV+R LS+ FR +  QS++++ ADARRA CSLS+SGGSV TGTR +GL  R N+D
Sbjct: 1   MEVSGAVSRHLSAFFRHDFDQSIELSRADARRASCSLSISGGSVGTGTRTLGLASRWNMD 60

Query: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL 120
           SSRF D+GHLRYYVGP C G RLKKEKEAVKKKLKLLKGL   S+TD+ES L+F  D   
Sbjct: 61  SSRFCDDGHLRYYVGPTCHGGRLKKEKEAVKKKLKLLKGL---SATDYESSLLFHLDHGS 120

Query: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSS 180
           IG+FQADRFSIEETREAL +QLQQLKSEEK+QKR++KLEKAKLKA RA+TIH+ SS SSS
Sbjct: 121 IGEFQADRFSIEETREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARAQTIHD-SSFSSS 180

Query: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV 240
           ESSDNE +M N SY+IRKALSQPFPDQ++TN I+ STLPPPLQ+QLLNS + N+GV  S 
Sbjct: 181 ESSDNEAHMINKSYRIRKALSQPFPDQYQTNAIQGSTLPPPLQTQLLNSKSGNVGV--ST 240

Query: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300
           SVGR+EVCMGNKCKKAGAAALMEEF RVMGADGAVCGCKCMGKCRDGPNVRVSGAL  Q 
Sbjct: 241 SVGRIEVCMGNKCKKAGAAALMEEFGRVMGADGAVCGCKCMGKCRDGPNVRVSGALDIQ- 300

Query: 301 CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 334
              NPLCIGVGVEDVGRIVA++LGQ+   +QSR
Sbjct: 301 ---NPLCIGVGVEDVGRIVAEYLGQERGQQQSR 323

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BLAST of Carg19593 vs. ExPASy Swiss-Prot
Match: Q9C5W0 (Diacylglycerol O-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=DGAT3 PE=1 SV=2)

HSP 1 Score: 153.7 bits (387), Expect = 3.7e-36
Identity = 131/356 (36.80%), Postives = 185/356 (51.97%), Query Frame = 0


Query: 23  VDVASADARRAPCSLSVSGGSV---------STGTRVIGLVGRR---NIDSSRFSDNGHL 82
           ++V+    R+ PC   VS GSV         S  TR +G   RR    + ++ F+D GH+
Sbjct: 1   MEVSGVVLRQIPC---VSSGSVAGLRLVSEFSGNTRTVGFRTRRFRGIVCNNEFADKGHV 60

Query: 83  RYYVGPNCQGRR------LKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDD--CLIG 142
            YY+ P   G        ++KEK+A+KKK K+LK LS   + D  S + F  D    L+G
Sbjct: 61  NYYIEPTRCGEEKEKVKVMEKEKKALKKKAKVLKSLS--KNLDMFSSIGFGLDPEAGLVG 120

Query: 143 DFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSSES 202
           + Q    +I E  E LVKQL+QLK+EEK  K++RK EKAK KA +  T  +S SSSSSES
Sbjct: 121 EIQTK--TISEATEILVKQLEQLKAEEKILKKQRKEEKAKAKAMKKMTEMDSESSSSSES 180

Query: 203 SDNE---GYMTNTSYQIRKALSQPFPDQWKTN-----------------------GIKES 262
           SD++   G + + S    KA     P Q +                          ++ S
Sbjct: 181 SDSDCDKGKVVDMSSLRNKAKPVLEPLQPEATVATLPRIQEDAISCKNTSEALQIALQTS 240

Query: 263 TLPPPLQSQLLNSDTKNIGVARSVSVGRVEVCMGNKCKKAGAAALMEEFERVM-GADGAV 322
           T+ P + +      T        + + RVEVCMG KCK++G A L++EF+R M G +G+ 
Sbjct: 241 TIFPSMANPGQTLKTVEAVSVVGLPLNRVEVCMGGKCKRSGGALLLDEFQRAMTGFEGSA 300

Query: 323 CGCKCMGKCRDGPNVRV---SGALAGQS--CSANPLCIGVGVEDVGRIVADHLGQQ 327
             CKCMGKCRDGPNVRV   + A+   S    +  LC+GVG++DV  IV     ++
Sbjct: 301 VACKCMGKCRDGPNVRVVKETDAVMTDSVRTPSKTLCVGVGLQDVETIVTSFFDEE 349

[Close]

BLAST of Carg19593 vs. ExPASy TrEMBL
Match: A0A6J1GV38 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662 GN=LOC111457454 PE=4 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.2e-178
Identity = 331/333 (99.40%), Postives = 331/333 (99.40%), Query Frame = 0


Query: 1   MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNID 60
           MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGT VIGLVGRRNID
Sbjct: 1   MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTHVIGLVGRRNID 60

Query: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL 120
           SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL
Sbjct: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL 120

Query: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSS 180
           IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATR KTIHESSSSSSS
Sbjct: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRVKTIHESSSSSSS 180

Query: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV 240
           ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV
Sbjct: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV 240

Query: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300
           SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS
Sbjct: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300

Query: 301 CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 334
           CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR
Sbjct: 301 CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 333

[Close]

BLAST of Carg19593 vs. ExPASy TrEMBL
Match: A0A6J1IRZ4 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita maxima OX=3661 GN=LOC111479426 PE=4 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.3e-171
Identity = 317/333 (95.20%), Postives = 324/333 (97.30%), Query Frame = 0


Query: 1   MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNID 60
           MEVSGAVTRRLSSLFRCEI QSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRN+D
Sbjct: 1   MEVSGAVTRRLSSLFRCEIVQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNVD 60

Query: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL 120
           SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKK KLLKGLSGYSSTDHESGLVFR DDCL
Sbjct: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKFKLLKGLSGYSSTDHESGLVFRLDDCL 120

Query: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSS 180
           IGD QADRFSIEETREAL++QLQQLKSEEKDQKR+RKLEKAKLKATRAKTIH+SSSSSSS
Sbjct: 121 IGDSQADRFSIEETREALIRQLQQLKSEEKDQKRKRKLEKAKLKATRAKTIHKSSSSSSS 180

Query: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV 240
           ESSDNEGYM NT Y+IRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVA+SV
Sbjct: 181 ESSDNEGYMINTRYRIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVAQSV 240

Query: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300
           SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALA QS
Sbjct: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALARQS 300

Query: 301 CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 334
           CSANPLCIGVGV DVGRIVADHLGQQG LKQSR
Sbjct: 301 CSANPLCIGVGVGDVGRIVADHLGQQGGLKQSR 333

[Close]

BLAST of Carg19593 vs. ExPASy TrEMBL
Match: A0A6J1GBG4 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662 GN=LOC111452646 PE=4 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 6.4e-124
Identity = 252/338 (74.56%), Postives = 277/338 (81.95%), Query Frame = 0


Query: 1   MEVSGAVTRRLSSLFRCEIAQ-SVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNI 60
           MEVSGAV+RRL  L R EIAQ S++V+  DARRAPCSLS+SGGSV T TR  GL  RRNI
Sbjct: 1   MEVSGAVSRRLPGLIRREIAQSSMEVSGHDARRAPCSLSISGGSVRTETRDFGLYVRRNI 60

Query: 61  DSSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDC 120
           +S RFSD+GH+RYYVG  C G RLKKEKEAVKKKLKLLKGLSG SS     G VFRFDD 
Sbjct: 61  NSPRFSDDGHVRYYVGTRCHGGRLKKEKEAVKKKLKLLKGLSGDSSAAFAFGFVFRFDDS 120

Query: 121 LIGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSS 180
            +G+FQADRFSIEETRE L +QLQQLK+EEK+QKR+RKLEKAKLKA R +T H+ SSSSS
Sbjct: 121 SVGEFQADRFSIEETREVLERQLQQLKAEEKEQKRKRKLEKAKLKAARLQTAHD-SSSSS 180

Query: 181 SESSDNEGYMTNTSYQIRKA----LSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIG 240
           SESS  EG MTNTSY+ RKA    LSQPF DQW+TN I+ S L PPLQ+  LNS+T N G
Sbjct: 181 SESSAIEGNMTNTSYRTRKANSPSLSQPFSDQWQTNAIQGSALAPPLQTHQLNSNTGNFG 240

Query: 241 VARSVSVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGA 300
           VARS SVGRVEVCMGNKCKKAGAAALMEEFERVMGAD  VCGCKCMG+CRDGPNVRVS +
Sbjct: 241 VARSSSVGRVEVCMGNKCKKAGAAALMEEFERVMGADSVVCGCKCMGRCRDGPNVRVSSS 300

Query: 301 LAGQSCSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 334
           L  ++ SANPLCIGVGVEDVGRIV DHLG QGE KQ R
Sbjct: 301 LDMETRSANPLCIGVGVEDVGRIVGDHLGGQGEHKQPR 337

[Close]

BLAST of Carg19593 vs. ExPASy TrEMBL
Match: E5GC43 (Diacylglycerol acyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 2.7e-122
Identity = 243/333 (72.97%), Postives = 277/333 (83.18%), Query Frame = 0


Query: 1   MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNID 60
           MEV GAV+R+L  LFR + +QSV+V  AD RR PCSLS+SG SV   TR +GLV RR +D
Sbjct: 1   MEVYGAVSRQLPGLFRRDFSQSVEVYDADTRRGPCSLSISGRSVGNETRALGLVSRRRMD 60

Query: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL 120
           SSRF D+G+LRYYVGP CQG  LKKEKEAVKKKLKLLKGL   S+TD+ES L+FRFD   
Sbjct: 61  SSRFCDDGYLRYYVGPTCQGGNLKKEKEAVKKKLKLLKGL---SATDYESSLLFRFDHGS 120

Query: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSS 180
           I +FQ+DRFSIE+TREAL +QLQQLK EEK+QKR++KLEKAKLKA R KTIH+ SSSSSS
Sbjct: 121 IDEFQSDRFSIEKTREALTRQLQQLKLEEKEQKRKKKLEKAKLKAARTKTIHD-SSSSSS 180

Query: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV 240
           ESSDNEG+M   SYQ++KALSQPFPDQ+KTN I  STLP PLQ+QLLNS T+NIGV RS 
Sbjct: 181 ESSDNEGHMNTRSYQLKKALSQPFPDQYKTNAIHVSTLPLPLQTQLLNSKTENIGVTRST 240

Query: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300
           SVGR+EVCMGNKCKKAGAAALMEEFERVMG + AVCGCKCMGKCRDGPNVRV G++  Q 
Sbjct: 241 SVGRIEVCMGNKCKKAGAAALMEEFERVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQ- 300

Query: 301 CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 334
              NPLCIGVGVEDV RIVA++LGQ+G   QSR
Sbjct: 301 ---NPLCIGVGVEDVDRIVAEYLGQKGGQTQSR 325

[Close]

BLAST of Carg19593 vs. ExPASy TrEMBL
Match: A0A5A7UAC7 (Diacylglycerol acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1769G00190 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 3.9e-121
Identity = 241/333 (72.37%), Postives = 276/333 (82.88%), Query Frame = 0


Query: 1   MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTRVIGLVGRRNID 60
           MEV GAV+R+L  LFR + +QSV+V  AD RR PCSLS+ G SV   TR +GLV RR +D
Sbjct: 1   MEVFGAVSRQLPGLFRRDFSQSVEVYDADTRRGPCSLSIYGRSVGNETRALGLVSRRRMD 60

Query: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL 120
           SSRF D+G+LRYYVGP CQG  LKKEKEAVKKKLKLLKGL   S+TD+ES L+FRFD   
Sbjct: 61  SSRFCDDGYLRYYVGPTCQGGNLKKEKEAVKKKLKLLKGL---SATDYESSLLFRFDHGS 120

Query: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSS 180
           I +FQ+DRFSIE+TREAL +QLQQLK EEK+QKR++KLEKAKLKA R KTIH+ SSSSSS
Sbjct: 121 IDEFQSDRFSIEKTREALTRQLQQLKLEEKEQKRKKKLEKAKLKAARTKTIHD-SSSSSS 180

Query: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV 240
           ESSDNEG+M   SY+++KALSQPFPDQ+KTN I  STLP PLQ+QLLNS T+NIGV RS 
Sbjct: 181 ESSDNEGHMNTRSYRLKKALSQPFPDQYKTNAIHVSTLPLPLQTQLLNSKTENIGVTRST 240

Query: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300
           SVGR+EVCMGNKCKKAGAAALMEEFERVMG + AVCGCKCMGKCRDGPNVRV G++  Q 
Sbjct: 241 SVGRIEVCMGNKCKKAGAAALMEEFERVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQ- 300

Query: 301 CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 334
              NPLCIGVGVEDV RIVA++LGQ+G   QSR
Sbjct: 301 ---NPLCIGVGVEDVDRIVAEYLGQKGGQTQSR 325

[Close]

BLAST of Carg19593 vs. TAIR 10
Match: AT1G48300.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; Has 96 Blast hits to 87 proteins in 37 species: Archae - 0; Bacteria - 2; Metazoa - 12; Fungi - 2; Plants - 65; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 133.7 bits (335), Expect = 2.8e-31
Identity = 108/278 (38.85%), Postives = 151/278 (54.32%), Query Frame = 0


Query: 83  LKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDD--CLIGDFQADRFSIEETREALVK 142
           ++KEK+A+KKK K+LK LS   + D  S + F  D    L+G+ Q    +I E  E LVK
Sbjct: 1   MEKEKKALKKKAKVLKSLS--KNLDMFSSIGFGLDPEAGLVGEIQTK--TISEATEILVK 60

Query: 143 QLQQLKSEEKDQKRRRKLEKAKLKATRAKTIHESSSSSSSESSDNE---GYMTNTSYQIR 202
           QL+QLK+EEK  K++RK EKAK KA +  T  +S SSSSSESSD++   G + + S    
Sbjct: 61  QLEQLKAEEKILKKQRKEEKAKAKAMKKMTEMDSESSSSSESSDSDCDKGKVVDMSSLRN 120

Query: 203 KALSQPFPDQWKTN-----------------------GIKESTLPPPLQSQLLNSDTKNI 262
           KA     P Q +                          ++ ST+ P + +      T   
Sbjct: 121 KAKPVLEPLQPEATVATLPRIQEDAISCKNTSEALQIALQTSTIFPSMANPGQTLKTVEA 180

Query: 263 GVARSVSVGRVEVCMGNKCKKAGAAALMEEFERVM-GADGAVCGCKCMGKCRDGPNVRV- 322
                + + RVEVCMG KCK++G A L++EF+R M G +G+   CKCMGKCRDGPNVRV 
Sbjct: 181 VSVVGLPLNRVEVCMGGKCKRSGGALLLDEFQRAMTGFEGSAVACKCMGKCRDGPNVRVV 240

Query: 323 --SGALAGQS--CSANPLCIGVGVEDVGRIVADHLGQQ 327
             + A+   S    +  LC+GVG++DV  IV     ++
Sbjct: 241 KETDAVMTDSVRTPSKTLCVGVGLQDVETIVTSFFDEE 274

The following BLAST results are available for this feature:

BLAST of Carg19593 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6582142.1	2.2e-179	100.00	Diacylglycerol O-acyltransferase 3, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022955425.1	2.5e-178	99.40	diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita moschata]	[more]
XP_023527748.1	3.0e-176	98.20	diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022979861.1	1.1e-170	95.20	diacylglycerol O-acyltransferase 3, cytosolic-like [Cucurbita maxima]	[more]
XP_038900889.1	3.1e-125	75.08	diacylglycerol O-acyltransferase 3 [Benincasa hispida]	[more]

BLAST of Carg19593 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C5W0	3.7e-36	36.80	Diacylglycerol O-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=DGAT3 PE=1...	[more]

BLAST of Carg19593 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GV38	1.2e-178	99.40	diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662...	[more]
A0A6J1IRZ4	5.3e-171	95.20	diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1GBG4	6.4e-124	74.56	diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662...	[more]
E5GC43	2.7e-122	72.97	Diacylglycerol acyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
A0A5A7UAC7	3.9e-121	72.37	Diacylglycerol acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]

BLAST of Carg19593 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48300.1	2.8e-31	38.85	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 128..162
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 243..332 e-value: 1.0E-7 score: 34.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 147..188
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 170..188
None	No IPR available	CDD	cd02980	TRX_Fd_family	coord: 244..322 e-value: 4.63E-9 score: 50.7036
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 244..323

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19593-RA	Carg19593-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019432	triglyceride biosynthetic process
cellular_component	GO:0005829	cytosol
molecular_function	GO:0004144	diacylglycerol O-acyltransferase activity