Carg19165 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg19165
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionmitogen-activated protein kinase kinase kinase 17-like
LocationCarg_Chr19: 8148277 .. 8149278 (+)
RNA-Seq ExpressionCarg19165
SyntenyCarg19165
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTGGACCCGACTAGCTGCCGTCGGCCACGGCTCCTTCGCCACCGTCTACCTCGCTGCTTCCTCATCTTCCGGCGACCTCTTCGCTGTCAAGTCCGCCGAGCTTCTCAATTCGGACTTTCTTGAAACAGAGCAGAGGATTCTTTCTTCGCTGTCCCACCCTTCAATTGTGGGCTACAAGGGATTCGACGTAACGACAGAAAATGGGAAGCTTATGTATAATCTCTTCATGGAGTACGCCGCTGGTGGCTCTCTCGCCGACGAAATCGTTCGGCGTGGCGGCCGGATTAAGGAGGTGACAGCGGCGTTTTACACGCGTCAAATTGTCAGAGGATTGGAGTACTTGCACAAGCGGGGTTTGGTGCATTGTGATGTCAAAGCTAGGAACATTTTAATCGGTGCAGATGGTTTGAAGATCGCTGATTTCGGATGCTCCAAATGGGTTCGTGAGTCGCCGGCAGCGGTCTGCGGAACGCCGATGTTTATGGCACCAGAGGTGGCGCGTGGGGAAACACAGGGGATTTCCTCTGACATATGGGCACTCGGGTGTACTTTAATCGAAATGGTGTCAGGCGCCCCGCCGTGGCCTACCGCCGGAGACCCAGTTTCGACGCTGTACCGGATCGCATATTCCGGCGACTCACCGGAAATTCCGAGCTTTTTATCGGAGAAAGCGAAGGATTTTTTAGGGAAATGCTTGAGAAGAAACCCGACAGAACGGTGGAGTGCGAATCAACTTCTTAAACATCCATTTTTGGGGGAACTGAGTTTTGGGTTCGAGATAATCAAGGAATTACATTCAGATTTCGAATCTCCGACGAGTATTTTGGATCAAGGCCCTTGGAACTCGTTAGAAGAAGGGTCGCAAACTCTGTTGAAAACAGAGACATGGGAGGACGACAGAATCAAATGGCTGTCGATGTTTTCAGGGGAAATCAAATGGGAAATGAGAGATGATAATTGGATCACAATCCGAAGCTGTGTTAATGATGAAAATTGA

mRNA sequence

ATGGCCTGGACCCGACTAGCTGCCGTCGGCCACGGCTCCTTCGCCACCGTCTACCTCGCTGCTTCCTCATCTTCCGGCGACCTCTTCGCTGTCAAGTCCGCCGAGCTTCTCAATTCGGACTTTCTTGAAACAGAGCAGAGGATTCTTTCTTCGCTGTCCCACCCTTCAATTGTGGGCTACAAGGGATTCGACGTAACGACAGAAAATGGGAAGCTTATGTATAATCTCTTCATGGAGTACGCCGCTGGTGGCTCTCTCGCCGACGAAATCGTTCGGCGTGGCGGCCGGATTAAGGAGGTGACAGCGGCGTTTTACACGCGTCAAATTGTCAGAGGATTGGAGTACTTGCACAAGCGGGGTTTGGTGCATTGTGATGTCAAAGCTAGGAACATTTTAATCGGTGCAGATGGTTTGAAGATCGCTGATTTCGGATGCTCCAAATGGGTTCGTGAGTCGCCGGCAGCGGTCTGCGGAACGCCGATGTTTATGGCACCAGAGGTGGCGCGTGGGGAAACACAGGGGATTTCCTCTGACATATGGGCACTCGGGTGTACTTTAATCGAAATGGTGTCAGGCGCCCCGCCGTGGCCTACCGCCGGAGACCCAGTTTCGACGCTGTACCGGATCGCATATTCCGGCGACTCACCGGAAATTCCGAGCTTTTTATCGGAGAAAGCGAAGGATTTTTTAGGGAAATGCTTGAGAAGAAACCCGACAGAACGGTGGAGTGCGAATCAACTTCTTAAACATCCATTTTTGGGGGAACTGAGTTTTGGGTTCGAGATAATCAAGGAATTACATTCAGATTTCGAATCTCCGACGAGTATTTTGGATCAAGGCCCTTGGAACTCGTTAGAAGAAGGGTCGCAAACTCTGTTGAAAACAGAGACATGGGAGGACGACAGAATCAAATGGCTGTCGATGTTTTCAGGGGAAATCAAATGGGAAATGAGAGATGATAATTGGATCACAATCCGAAGCTGTGTTAATGATGAAAATTGA

Coding sequence (CDS)

ATGGCCTGGACCCGACTAGCTGCCGTCGGCCACGGCTCCTTCGCCACCGTCTACCTCGCTGCTTCCTCATCTTCCGGCGACCTCTTCGCTGTCAAGTCCGCCGAGCTTCTCAATTCGGACTTTCTTGAAACAGAGCAGAGGATTCTTTCTTCGCTGTCCCACCCTTCAATTGTGGGCTACAAGGGATTCGACGTAACGACAGAAAATGGGAAGCTTATGTATAATCTCTTCATGGAGTACGCCGCTGGTGGCTCTCTCGCCGACGAAATCGTTCGGCGTGGCGGCCGGATTAAGGAGGTGACAGCGGCGTTTTACACGCGTCAAATTGTCAGAGGATTGGAGTACTTGCACAAGCGGGGTTTGGTGCATTGTGATGTCAAAGCTAGGAACATTTTAATCGGTGCAGATGGTTTGAAGATCGCTGATTTCGGATGCTCCAAATGGGTTCGTGAGTCGCCGGCAGCGGTCTGCGGAACGCCGATGTTTATGGCACCAGAGGTGGCGCGTGGGGAAACACAGGGGATTTCCTCTGACATATGGGCACTCGGGTGTACTTTAATCGAAATGGTGTCAGGCGCCCCGCCGTGGCCTACCGCCGGAGACCCAGTTTCGACGCTGTACCGGATCGCATATTCCGGCGACTCACCGGAAATTCCGAGCTTTTTATCGGAGAAAGCGAAGGATTTTTTAGGGAAATGCTTGAGAAGAAACCCGACAGAACGGTGGAGTGCGAATCAACTTCTTAAACATCCATTTTTGGGGGAACTGAGTTTTGGGTTCGAGATAATCAAGGAATTACATTCAGATTTCGAATCTCCGACGAGTATTTTGGATCAAGGCCCTTGGAACTCGTTAGAAGAAGGGTCGCAAACTCTGTTGAAAACAGAGACATGGGAGGACGACAGAATCAAATGGCTGTCGATGTTTTCAGGGGAAATCAAATGGGAAATGAGAGATGATAATTGGATCACAATCCGAAGCTGTGTTAATGATGAAAATTGA

Protein sequence

MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGYKGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRGLVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIWALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTERWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWEDDRIKWLSMFSGEIKWEMRDDNWITIRSCVNDEN
Homology
BLAST of Carg19165 vs. NCBI nr
Match: KAG7011904.1 (Mitogen-activated protein kinase kinase kinase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 684.1 bits (1764), Expect = 6.0e-193
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY
Sbjct: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60

Query: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120
           KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG
Sbjct: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120

Query: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180
           LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW
Sbjct: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180

Query: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240
           ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE
Sbjct: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240

Query: 241 RWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300
           RWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED
Sbjct: 241 RWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300

Query: 301 DRIKWLSMFSGEIKWEMRDDNWITIRSCVNDEN 334
           DRIKWLSMFSGEIKWEMRDDNWITIRSCVNDEN
Sbjct: 301 DRIKWLSMFSGEIKWEMRDDNWITIRSCVNDEN 333

BLAST of Carg19165 vs. NCBI nr
Match: KAG6572287.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 676.0 bits (1743), Expect = 1.6e-190
Identity = 330/333 (99.10%), Postives = 332/333 (99.70%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY
Sbjct: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60

Query: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120
           KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG
Sbjct: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120

Query: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180
           LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW
Sbjct: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180

Query: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240
           ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE
Sbjct: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240

Query: 241 RWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300
           RWSAN+LLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED
Sbjct: 241 RWSANRLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300

Query: 301 DRIKWLSMFSGEIKWEMRDDNWITIRSCVNDEN 334
           DRIK LSMFSGEIKWEMRDDNWITIRSCVND+N
Sbjct: 301 DRIKRLSMFSGEIKWEMRDDNWITIRSCVNDDN 333

BLAST of Carg19165 vs. NCBI nr
Match: XP_022952534.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata])

HSP 1 Score: 674.9 bits (1740), Expect = 3.7e-190
Identity = 329/333 (98.80%), Postives = 332/333 (99.70%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY
Sbjct: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60

Query: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120
           KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG
Sbjct: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120

Query: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180
           LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW
Sbjct: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180

Query: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240
           ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE
Sbjct: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240

Query: 241 RWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300
           RWSAN+LLKHPFLGELSFGFEII+ELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED
Sbjct: 241 RWSANRLLKHPFLGELSFGFEIIRELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300

Query: 301 DRIKWLSMFSGEIKWEMRDDNWITIRSCVNDEN 334
           DRIK LSMFSGEIKWEMRDDNWITIRSCVND+N
Sbjct: 301 DRIKRLSMFSGEIKWEMRDDNWITIRSCVNDDN 333

BLAST of Carg19165 vs. NCBI nr
Match: XP_023554546.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 661.8 bits (1706), Expect = 3.2e-186
Identity = 322/333 (96.70%), Postives = 326/333 (97.90%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQR LSSLSHPSIVGY
Sbjct: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRTLSSLSHPSIVGY 60

Query: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120
           KGFDVT ENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG
Sbjct: 61  KGFDVTAENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120

Query: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180
           LVHCDVKA NILIG DGLKIADFGCSKWVRESPAA+CGTPMFMAPEVARGETQGISSDIW
Sbjct: 121 LVHCDVKANNILIGGDGLKIADFGCSKWVRESPAAICGTPMFMAPEVARGETQGISSDIW 180

Query: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240
           ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRR+PTE
Sbjct: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRDPTE 240

Query: 241 RWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300
           RWSA+QLLKHPFLGELSFGFEI KELHSDFESPTSILDQGPWNSLEEG QTLLKTETWED
Sbjct: 241 RWSASQLLKHPFLGELSFGFEITKELHSDFESPTSILDQGPWNSLEEGLQTLLKTETWED 300

Query: 301 DRIKWLSMFSGEIKWEMRDDNWITIRSCVNDEN 334
           DRIK LSMFSGEIKWEMRDDNWITIRSCVND+N
Sbjct: 301 DRIKRLSMFSGEIKWEMRDDNWITIRSCVNDDN 333

BLAST of Carg19165 vs. NCBI nr
Match: XP_022969170.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita maxima])

HSP 1 Score: 652.5 bits (1682), Expect = 1.9e-183
Identity = 318/333 (95.50%), Postives = 325/333 (97.60%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFL+TEQRILSSLSHPSIVGY
Sbjct: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLQTEQRILSSLSHPSIVGY 60

Query: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120
           KGFDVTTENGKLMYNLFMEYAA GSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG
Sbjct: 61  KGFDVTTENGKLMYNLFMEYAACGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120

Query: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180
           LVHCDVKA+NILIG DGLKIADFGCSKWV ESPAAVCGTPMFMAPEVARGETQGISSDIW
Sbjct: 121 LVHCDVKAKNILIGGDGLKIADFGCSKWVLESPAAVCGTPMFMAPEVARGETQGISSDIW 180

Query: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240
           ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLS  AKDFLGKCLRR+P E
Sbjct: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSATAKDFLGKCLRRDPAE 240

Query: 241 RWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300
           RWS +QLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED
Sbjct: 241 RWSTSQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300

Query: 301 DRIKWLSMFSGEIKWEMRDDNWITIRSCVNDEN 334
           DRIK LS+FSGEIKW+MRDDNWITIRSCVND+N
Sbjct: 301 DRIKRLSVFSGEIKWKMRDDNWITIRSCVNDDN 333

BLAST of Carg19165 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.0e-82
Identity = 169/351 (48.15%), Postives = 225/351 (64.10%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           M WTR   +G GS ATVY AA  +S ++ AVKS+E+  S+FL+ E +ILSSLS P ++GY
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  KGFDVTTE-NGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKR 120
           +G +   E NG +MYNL MEYA  G+L D   + GGR+ E     YTR I++GLEY+H +
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GLVHCDVKARNILIGADG-LKIADFGCSKWVR---ESPAAVCGTPMFMAPEVARGETQGI 180
           G+VHCDVK  N++I   G  KIADFGC+K V    ESP  V GTP FMAPEVARGE QG 
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESP--VMGTPAFMAPEVARGEKQGK 180

Query: 181 SSDIWALGCTLIEMVSGAPPWPTAG---DPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGK 240
            SDIWA+GCT+IEMV+G+PPW  A    DPVS LYR+ YS ++PE+P  L+E+AKDFL K
Sbjct: 181 ESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEK 240

Query: 241 CLRRNPTERWSANQLLKHPFL-GELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQ- 300
           CL+R   ERW+A QLL HPFL  +      ++  L S+  SPTS+ DQ  W S+EE  + 
Sbjct: 241 CLKREANERWTATQLLNHPFLTTKPDIEPVLVPGLISN--SPTSVTDQTFWRSVEEEEEE 300

Query: 301 --TLLKTETWEDDRIKWLSMFSGEI------------KWEMRDDNWITIRS 328
               ++ ++ + DR+     +S  I            + +M   +WI +R+
Sbjct: 301 ETEEIQKDSRDLDRLSLWGCYSERIGRLKCVGGLDGTRCDMEGGDWIMVRA 347

BLAST of Carg19165 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.0e-82
Identity = 169/341 (49.56%), Postives = 221/341 (64.81%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           M WTR   +G GS ATV  A    SG+  AVKSAE   S+FL+ E +ILSSL+ P ++GY
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  KGFDVTTE----NGK-LMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEY 120
           +G ++T E    NG+   Y+L MEYA  G+L D   + GG I E     YTRQI+ GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 LH-KRGLVHCDVKARNILIGADG-LKIADFGCSKWVR-ESPAAVCGTPMFMAPEVARGET 180
           +H  +G+ HCD+K  N+L+G +G  KIADFGC+KWV  E    V GTP FMAPE ARGE 
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPVRGTPAFMAPEAARGER 180

Query: 181 QGISSDIWALGCTLIEMVSGAPPWPTAG--DPVSTLYRIAYSGDSPEIPSFLSEKAKDFL 240
           QG  SDIWA+GCT+IEMV+G+ PW  A   DPVS LYR+ Y G+ PE+P  L+E+AKDFL
Sbjct: 181 QGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDFL 240

Query: 241 GKCLRRNPTERWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEE--G 300
           GKCL++  TERW+A+QLL HPFL  ++   E++  L ++  SPTS+ DQ  W S+EE   
Sbjct: 241 GKCLKKEATERWTASQLLNHPFL--VNKEPELVTGLVTN--SPTSVTDQMFWRSVEEEVS 300

Query: 301 SQTLLKTETWEDDRI---KWLSMFSGEIKWEMRDDNWITIR 327
                  E  ED+RI    W+     E  W++  ++WIT+R
Sbjct: 301 EDRSSWWECHEDERIGVLSWIGHVVVESTWDLDGEDWITVR 337

BLAST of Carg19165 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 7.7e-50
Identity = 126/347 (36.31%), Postives = 183/347 (52.74%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASS-SSGD---LFAVKSAELLNSDFLETEQRILSSLSH-P 60
           M W R   +G G+F+TV  A  S +SGD   L AVKS +   +  L  E+ +L SL   P
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  SIVGYKGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGR-IKEVTAAFYTRQIVRGLE 120
            I+   G D T ENG+ M+NL +EYA+ GSLA  + + GG  + E T   +T  ++RGL 
Sbjct: 61  EIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLR 120

Query: 121 YLHKRGLVHCDVKARNILIGADG-LKIADFGCSKWVRESPAA------VCGTPMFMAPEV 180
           ++H +G  HCD+K  NIL+  DG +KIADFG +  V     A      + GTP++MAPE 
Sbjct: 121 HIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPEC 180

Query: 181 ARGETQGISSDIWALGCTLIEMVSGAPPWPT--AGDPVSTLYRIAYSGDSPEIPSFLSEK 240
                 G ++D+WALGC ++EM SG   W        +S L RI    + P+IP  LSE+
Sbjct: 181 VNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEE 240

Query: 241 AKDFLGKCLRRNPTERWSANQLLKHPFL-----GELSFGFEI-IKELHSDFESPTSILDQ 300
            KDFL KC  ++P +RW+A  LL H F+      +    F + +K+      SP    + 
Sbjct: 241 GKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFEF 300

Query: 301 GPWNSLEEGSQTLLKTETWEDDRIKWLSMFSGEIKWEMRDDNWITIR 327
             W+S      TL    +++    +  S+ SG I       +W+T+R
Sbjct: 301 DDWDSF-----TLDSNPSFDSPVERLGSLVSGSIPDWSVGGSWLTVR 342

BLAST of Carg19165 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 5.0e-49
Identity = 106/274 (38.69%), Postives = 157/274 (57.30%), Query Frame = 0

Query: 3   WTRLAAVGHGSFATVYLAASSSSGDLFAVKSAEL--LNSD--------FLETEQRILSSL 62
           W +   +G G + +VYL  +  +G+LFAVK  E+  +NSD            E  ++ SL
Sbjct: 170 WQKGQILGRGGYGSVYLGLNKDTGELFAVKQLEIVDINSDPKLKNMILSFSKEIEVMRSL 229

Query: 63  SHPSIVGYKGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRG 122
            H +IV Y G    T   +   ++F+EY  GGS++  ++ + G   E     YT+QI++G
Sbjct: 230 RHDNIVRYLG----TSLDQSFLSVFLEYIPGGSIS-SLLGKFGAFSENVIKVYTKQILQG 289

Query: 123 LEYLHKRGLVHCDVKARNILIGADGL-KIADFGCSK---WVRESPAAVCGTPMFMAPEVA 182
           L +LH   ++H D+K  NILI   G+ K++DFGCSK    +     ++ GTP +MAPEV 
Sbjct: 290 LSFLHANSIIHRDIKGANILIDTKGIVKLSDFGCSKSFSGIVSQFKSMQGTPYWMAPEVI 349

Query: 183 RGETQGISSDIWALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKD 242
           +    G SSDIW+LGC ++EM +  PPW    +  + +Y IA S   P IPS +S++A D
Sbjct: 350 KQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEAFD 409

Query: 243 FLGKCLRRNPTERWSANQLLKHPFLGELSFGFEI 263
           FL  C +R+P ER  ANQLLKHPF+  L    ++
Sbjct: 410 FLNLCFKRDPKERPDANQLLKHPFIMNLDDNIQL 438

BLAST of Carg19165 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 6.1e-47
Identity = 106/268 (39.55%), Postives = 153/268 (57.09%), Query Frame = 0

Query: 3   WTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDF-----------LETEQRILSS 62
           W +   +G G+F  VY+  +  SG+L A+K   +  +             LE E  +L +
Sbjct: 80  WRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASRERAQAHVRELEEEVNLLKN 139

Query: 63  LSHPSIVGYKGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVR 122
           LSHP+IV Y G     E G L  N+ +E+  GGS++  ++ + G   E     YT+Q++ 
Sbjct: 140 LSHPNIVRYLG--TAREAGSL--NILLEFVPGGSIS-SLLGKFGSFPESVIRMYTKQLLL 199

Query: 123 GLEYLHKRGLVHCDVKARNILIGADG-LKIADFGCSKWVRE-----SPAAVCGTPMFMAP 182
           GLEYLHK G++H D+K  NIL+   G +K+ADFG SK V E        ++ GTP +MAP
Sbjct: 200 GLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMAP 259

Query: 183 EVARGETQGISSDIWALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEK 242
           EV        S+DIW++GCT+IEM +G PPW      V+ L+ I  +   P IP  LS +
Sbjct: 260 EVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEHLSAE 319

Query: 243 AKDFLGKCLRRNPTERWSANQLLKHPFL 254
           +KDFL KCL++ P  R SA+ LL+HPF+
Sbjct: 320 SKDFLLKCLQKEPHLRHSASNLLQHPFV 342

BLAST of Carg19165 vs. ExPASy TrEMBL
Match: A0A6J1GLZ4 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111455196 PE=3 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 1.8e-190
Identity = 329/333 (98.80%), Postives = 332/333 (99.70%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY
Sbjct: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60

Query: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120
           KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG
Sbjct: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120

Query: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180
           LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW
Sbjct: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180

Query: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240
           ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE
Sbjct: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240

Query: 241 RWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300
           RWSAN+LLKHPFLGELSFGFEII+ELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED
Sbjct: 241 RWSANRLLKHPFLGELSFGFEIIRELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300

Query: 301 DRIKWLSMFSGEIKWEMRDDNWITIRSCVNDEN 334
           DRIK LSMFSGEIKWEMRDDNWITIRSCVND+N
Sbjct: 301 DRIKRLSMFSGEIKWEMRDDNWITIRSCVNDDN 333

BLAST of Carg19165 vs. ExPASy TrEMBL
Match: A0A6J1HZ76 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=3661 GN=LOC111468247 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 9.4e-184
Identity = 318/333 (95.50%), Postives = 325/333 (97.60%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFL+TEQRILSSLSHPSIVGY
Sbjct: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLQTEQRILSSLSHPSIVGY 60

Query: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120
           KGFDVTTENGKLMYNLFMEYAA GSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG
Sbjct: 61  KGFDVTTENGKLMYNLFMEYAACGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120

Query: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180
           LVHCDVKA+NILIG DGLKIADFGCSKWV ESPAAVCGTPMFMAPEVARGETQGISSDIW
Sbjct: 121 LVHCDVKAKNILIGGDGLKIADFGCSKWVLESPAAVCGTPMFMAPEVARGETQGISSDIW 180

Query: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240
           ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLS  AKDFLGKCLRR+P E
Sbjct: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSATAKDFLGKCLRRDPAE 240

Query: 241 RWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300
           RWS +QLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED
Sbjct: 241 RWSTSQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300

Query: 301 DRIKWLSMFSGEIKWEMRDDNWITIRSCVNDEN 334
           DRIK LS+FSGEIKW+MRDDNWITIRSCVND+N
Sbjct: 301 DRIKRLSVFSGEIKWKMRDDNWITIRSCVNDDN 333

BLAST of Carg19165 vs. ExPASy TrEMBL
Match: A0A0A0K6U1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G043040 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.4e-149
Identity = 263/333 (78.98%), Postives = 292/333 (87.69%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           MAWTR + VG+GS ATVYLA+++SSG LFAVKSAELL SDFL+ E+R+LSSLS+PSIVGY
Sbjct: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIERRVLSSLSNPSIVGY 60

Query: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120
           KGFDVT ENGKLMYNLFMEYAAGG+LADEI RRGGRIKE TAAFYTR+IVRGLEYLHK+G
Sbjct: 61  KGFDVTRENGKLMYNLFMEYAAGGTLADEIFRRGGRIKEATAAFYTREIVRGLEYLHKQG 120

Query: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180
           LVHCD+KA+NILI  DGLKIADFGCS+WV ES A + GTPMFMAPEVARGE QGISSDIW
Sbjct: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCESEAVIGGTPMFMAPEVARGEKQGISSDIW 180

Query: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240
           ALGCTLIEMV+GAPPW    DPVS LYRI YSG+SPEIPSFLSEK KDFL KCLRR  TE
Sbjct: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240

Query: 241 RWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300
           RWSA+QLL+HPFLGELS G E IKELH   ESPTSILDQ  WNSLEE S+TLL+TE W+D
Sbjct: 241 RWSASQLLEHPFLGELSSGLEEIKELHLYSESPTSILDQSLWNSLEEESETLLRTEQWDD 300

Query: 301 DRIKWLSMFSGEIKWEMRDDNWITIRSCVNDEN 334
           DRI+ L+ FSGEIKWE+ D+NWITIRS V+ E+
Sbjct: 301 DRIERLATFSGEIKWELGDENWITIRSYVDGED 333

BLAST of Carg19165 vs. ExPASy TrEMBL
Match: A0A5A7T9T3 (Mitogen-activated protein kinase kinase kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G004350 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 6.4e-148
Identity = 265/338 (78.40%), Postives = 292/338 (86.39%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           MAWTR + VG+GS ATVYLA+++SSG+LFAVKSAELL SDFL+TEQRILSSLSHPSIVGY
Sbjct: 1   MAWTRRSPVGNGSSATVYLASTASSGELFAVKSAELLKSDFLKTEQRILSSLSHPSIVGY 60

Query: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120
           +GFDVT ENGKLMYNLFMEYA GG+L DEI RRGGRIKE TAAFYTR+IVRGLEYLHK+G
Sbjct: 61  RGFDVTRENGKLMYNLFMEYADGGTLTDEIFRRGGRIKEATAAFYTREIVRGLEYLHKQG 120

Query: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180
           LVHCD+KA+NILI  DGLKIADFGCSKWV E+ A   GTPMFMAPEVARGE QGISSDIW
Sbjct: 121 LVHCDIKAKNILIAGDGLKIADFGCSKWVCEAEAVKGGTPMFMAPEVARGEKQGISSDIW 180

Query: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240
           ALGCTLIEMV+GA PW T  DPVS LYRI YSG+SPE+PSFLSEK KDFL KCLRR  TE
Sbjct: 181 ALGCTLIEMVTGALPWKTTDDPVSVLYRIGYSGESPEVPSFLSEKGKDFLRKCLRREATE 240

Query: 241 RWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETW-- 300
           RWSA+QLL+HPFLGELS G E IKELHS  ESPTSILDQ  WNSLEE S+TLLKTE W  
Sbjct: 241 RWSASQLLEHPFLGELSSGLEEIKELHSYSESPTSILDQSLWNSLEEESETLLKTEQWDD 300

Query: 301 ---EDDRIKWLSMFSGEIKWEMRDDNWITIRSCVNDEN 334
              +DDRI+ L+ FSGEIKWE+ D+NWITIRS V+ E+
Sbjct: 301 EDEDDDRIERLATFSGEIKWELGDENWITIRSYVDGED 338

BLAST of Carg19165 vs. ExPASy TrEMBL
Match: A0A1S3C1N1 (mitogen-activated protein kinase kinase kinase 2 OS=Cucumis melo OX=3656 GN=LOC103495608 PE=3 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 4.1e-147
Identity = 264/338 (78.11%), Postives = 290/338 (85.80%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           MAWTR + VG+GS ATVYLA+++SSG+LFAVKSAELL SDFL+TEQRILSSLSHPSIVGY
Sbjct: 1   MAWTRRSPVGNGSSATVYLASTASSGELFAVKSAELLKSDFLKTEQRILSSLSHPSIVGY 60

Query: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120
           +GFDVT ENGKLMYNLFMEYA GG+L DEI RRGGRIKE TAAFYTR+IVRGLEYLHK+G
Sbjct: 61  RGFDVTRENGKLMYNLFMEYADGGTLTDEIFRRGGRIKEATAAFYTREIVRGLEYLHKQG 120

Query: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180
           LVHCD+KA+NILI  DGLKIADFGCSKWV E+ A   GTPMFMAPEVARGE QGISSDIW
Sbjct: 121 LVHCDIKAKNILIAGDGLKIADFGCSKWVCEAEAVKGGTPMFMAPEVARGEKQGISSDIW 180

Query: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240
           ALGCTLIEMV+GA PW T  DPVS LYRI YSG+SPE+PSFLSEK  DFL KCLRR  TE
Sbjct: 181 ALGCTLIEMVTGALPWKTTDDPVSVLYRIGYSGESPEVPSFLSEKGNDFLRKCLRREATE 240

Query: 241 RWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETW-- 300
           RWSA+QLL+HPFLGELS G E IKELHS  ESPTSILDQ  WNSLEE S+TLLKTE W  
Sbjct: 241 RWSASQLLEHPFLGELSSGLEEIKELHSYSESPTSILDQSLWNSLEEESETLLKTEQWDD 300

Query: 301 ---EDDRIKWLSMFSGEIKWEMRDDNWITIRSCVNDEN 334
              +DDRI+ L+ FSGEIKWE  D+NWITIRS V+ E+
Sbjct: 301 EDEDDDRIERLATFSGEIKWEFGDENWITIRSYVDGED 338

BLAST of Carg19165 vs. TAIR 10
Match: AT1G05100.1 (mitogen-activated protein kinase kinase kinase 18 )

HSP 1 Score: 307.4 bits (786), Expect = 1.4e-83
Identity = 169/341 (49.56%), Postives = 221/341 (64.81%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           M WTR   +G GS ATV  A    SG+  AVKSAE   S+FL+ E +ILSSL+ P ++GY
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  KGFDVTTE----NGK-LMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEY 120
           +G ++T E    NG+   Y+L MEYA  G+L D   + GG I E     YTRQI+ GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 LH-KRGLVHCDVKARNILIGADG-LKIADFGCSKWVR-ESPAAVCGTPMFMAPEVARGET 180
           +H  +G+ HCD+K  N+L+G +G  KIADFGC+KWV  E    V GTP FMAPE ARGE 
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPVRGTPAFMAPEAARGER 180

Query: 181 QGISSDIWALGCTLIEMVSGAPPWPTAG--DPVSTLYRIAYSGDSPEIPSFLSEKAKDFL 240
           QG  SDIWA+GCT+IEMV+G+ PW  A   DPVS LYR+ Y G+ PE+P  L+E+AKDFL
Sbjct: 181 QGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDFL 240

Query: 241 GKCLRRNPTERWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEE--G 300
           GKCL++  TERW+A+QLL HPFL  ++   E++  L ++  SPTS+ DQ  W S+EE   
Sbjct: 241 GKCLKKEATERWTASQLLNHPFL--VNKEPELVTGLVTN--SPTSVTDQMFWRSVEEEVS 300

Query: 301 SQTLLKTETWEDDRI---KWLSMFSGEIKWEMRDDNWITIR 327
                  E  ED+RI    W+     E  W++  ++WIT+R
Sbjct: 301 EDRSSWWECHEDERIGVLSWIGHVVVESTWDLDGEDWITVR 337

BLAST of Carg19165 vs. TAIR 10
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17 )

HSP 1 Score: 307.4 bits (786), Expect = 1.4e-83
Identity = 169/351 (48.15%), Postives = 225/351 (64.10%), Query Frame = 0

Query: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60
           M WTR   +G GS ATVY AA  +S ++ AVKS+E+  S+FL+ E +ILSSLS P ++GY
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  KGFDVTTE-NGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKR 120
           +G +   E NG +MYNL MEYA  G+L D   + GGR+ E     YTR I++GLEY+H +
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GLVHCDVKARNILIGADG-LKIADFGCSKWVR---ESPAAVCGTPMFMAPEVARGETQGI 180
           G+VHCDVK  N++I   G  KIADFGC+K V    ESP  V GTP FMAPEVARGE QG 
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESP--VMGTPAFMAPEVARGEKQGK 180

Query: 181 SSDIWALGCTLIEMVSGAPPWPTAG---DPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGK 240
            SDIWA+GCT+IEMV+G+PPW  A    DPVS LYR+ YS ++PE+P  L+E+AKDFL K
Sbjct: 181 ESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEK 240

Query: 241 CLRRNPTERWSANQLLKHPFL-GELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQ- 300
           CL+R   ERW+A QLL HPFL  +      ++  L S+  SPTS+ DQ  W S+EE  + 
Sbjct: 241 CLKREANERWTATQLLNHPFLTTKPDIEPVLVPGLISN--SPTSVTDQTFWRSVEEEEEE 300

Query: 301 --TLLKTETWEDDRIKWLSMFSGEI------------KWEMRDDNWITIRS 328
               ++ ++ + DR+     +S  I            + +M   +WI +R+
Sbjct: 301 ETEEIQKDSRDLDRLSLWGCYSERIGRLKCVGGLDGTRCDMEGGDWIMVRA 347

BLAST of Carg19165 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 304.3 bits (778), Expect = 1.2e-82
Identity = 165/350 (47.14%), Postives = 218/350 (62.29%), Query Frame = 0

Query: 3   WTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGYKG 62
           W R   +G GS ATV L   ++SGD FAVKSAE  +S FL+ EQ ILS LS P IV Y G
Sbjct: 6   WIRGPIIGRGSTATVSLGI-TNSGDFFAVKSAEFSSSAFLQREQSILSKLSSPYIVKYIG 65

Query: 63  FDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRGLV 122
            +VT EN KLMYNL MEY +GGSL D I   GG++ E     YTRQI++GL YLH +G+V
Sbjct: 66  SNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYLHDQGIV 125

Query: 123 HCDVKARNILIGADGLKIADFGCSKWVRESP-AAVCGTPMFMAPEVARGETQGISSDIWA 182
           HCDVK++N++IG +  KI D GC+K V E+      GTP FM+PEVARGE Q   +D+WA
Sbjct: 126 HCDVKSQNVMIGGEIAKIVDLGCAKTVEENENLEFSGTPAFMSPEVARGEEQSFPADVWA 185

Query: 183 LGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTER 242
           LGCT+IEM +G+ PWP   D V+ +Y+I ++G+SP IP +LSEK +DFL KCLR++P +R
Sbjct: 186 LGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSEKGQDFLRKCLRKDPKQR 245

Query: 243 WSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTE----- 302
           W+  +LL+HPFL E     +      +   SP+++LDQ  W+  E      +K +     
Sbjct: 246 WTVEELLQHPFLDEEDNDSDQTGNCLNS-SSPSTVLDQRFWDLCETSRSRFIKEDHEDPF 305

Query: 303 ------TWED-----DRIKWLS--MFSGEIKWEMRDDNWITIRSCVNDEN 334
                  W+D     DRIK L+    SGE  WE   + WI +R  +   N
Sbjct: 306 ANSTNFLWDDDSLPGDRIKKLAGDESSGEPDWE--TNGWIEVRGEIEKRN 351

BLAST of Carg19165 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 297.7 bits (761), Expect = 1.1e-80
Identity = 162/342 (47.37%), Postives = 223/342 (65.20%), Query Frame = 0

Query: 3   WTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGYKG 62
           WTR   +G GS ATV +A  SSSG+LFAVKSA+L +S  L+ EQ ILS+LS P +V Y G
Sbjct: 5   WTRGPIIGRGSTATVSIAI-SSSGELFAVKSADLSSSSLLQKEQSILSTLSSPHMVKYIG 64

Query: 63  FDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRGLV 122
             +T E+  L+YN+ MEY +GG+L D I   GG++ E     YTRQI+ GL YLH+RG+V
Sbjct: 65  TGLTRESNGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLVYLHERGIV 124

Query: 123 HCDVKARNILIGADG-LKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIWA 182
           HCD+K+ N+L+  +G LKIAD GC+K V +S  +  GTP FMAPEVARGE Q   +D+WA
Sbjct: 125 HCDLKSHNVLVEENGVLKIADMGCAKSVDKSEFS--GTPAFMAPEVARGEEQRFPADVWA 184

Query: 183 LGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTER 242
           LGCT+IEM++G+ PWP   D V+ +Y+I +SG+SP IP+++S+KAKDFL  CL+ +  +R
Sbjct: 185 LGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPAWISDKAKDFLKNCLKEDQKQR 244

Query: 243 WSANQLLKHPFL--GELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWE 302
           W+  +LLKHPFL   E S   + +K   S   SP+++LDQ  W+S E     L+  +  +
Sbjct: 245 WTVEELLKHPFLDDDEESQTSDCLKNKTS---SPSTVLDQRFWDSCESSKSHLVSIDHED 304

Query: 303 D------------DRIKWLS--MFSGEIKWEMRDD-NWITIR 327
                        DRI+ L+   FS  + W+  DD  WI +R
Sbjct: 305 PFAEYSESLDSPADRIEKLAGDEFSSLLDWDTEDDGGWIQVR 340

BLAST of Carg19165 vs. TAIR 10
Match: AT1G07150.1 (mitogen-activated protein kinase kinase kinase 13 )

HSP 1 Score: 214.2 bits (544), Expect = 1.6e-55
Identity = 137/346 (39.60%), Postives = 194/346 (56.07%), Query Frame = 0

Query: 3   WTRLAAVGHGSFATVYLAASSSSGDLFAVKSAEL-----LNSDFLETEQRILSSLS-HPS 62
           W R A +G G F  V  A S ++G++FAVKS +L       S+ LE E  +  SL  HP 
Sbjct: 23  WVRGACIGRGCFGAVSTAISKTNGEVFAVKSVDLATSLPTQSESLENEISVFRSLKPHPY 82

Query: 63  IVGYKGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVT-AAFYTRQIVRGLEY 122
           IV + G  V+ E      NL++EY   G +A    R GG+I++ T    YT  +V  L +
Sbjct: 83  IVKFLGDGVSKEGTTTFRNLYLEYLPNGDVASH--RAGGKIEDETLLQRYTACLVSALRH 142

Query: 123 LHKRGLVHCDVKARNILIGADGL-KIADFGCSKWVRESPAAVC--GTPMFMAPEVARGET 182
           +H +G VHCDVKARNIL+    + K+ADFG +  +    A +   G+P++MAPEV R E 
Sbjct: 143 VHSQGFVHCDVKARNILVSQSSMVKLADFGSAFRIHTPRALITPRGSPLWMAPEVIRREY 202

Query: 183 QGISSDIWALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGK 242
           QG  SD+W+LGCT+IEM +G P W   G  + +L RI++S + P  PS LSE  +DFL K
Sbjct: 203 QGPESDVWSLGCTIIEMFTGKPAWEDHG--IDSLSRISFSDELPVFPSKLSEIGRDFLEK 262

Query: 243 CLRRNPTERWSANQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPW-NS---LEEG 302
           CL+R+P +RWS +QLL+HPFL +                SP  +LD   W NS   LEE 
Sbjct: 263 CLKRDPNQRWSCDQLLQHPFLSQ-------CHNSSPTESSPRCVLD---WVNSGFDLEEE 322

Query: 303 SQTLLKTETWED--DRIKWLSMFSGEIKWEMRDDNWITIRSCVNDE 333
            + + ++E +ED    I      +G + WE   D W+ +R   ++E
Sbjct: 323 EEEVGRSE-FEDAAKAIICNLATTGGVIWE--SDGWVEVRCHASEE 351

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7011904.16.0e-193100.00Mitogen-activated protein kinase kinase kinase 18, partial [Cucurbita argyrosper... [more]
KAG6572287.11.6e-19099.10Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper... [more]
XP_022952534.13.7e-19098.80mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata][more]
XP_023554546.13.2e-18696.70mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pe... [more]
XP_022969170.11.9e-18395.50mitogen-activated protein kinase kinase kinase 17-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O808882.0e-8248.15Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q9ZVP52.0e-8249.56Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
Q9SND67.7e-5036.31Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
Q54R825.0e-4938.69Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Q405416.1e-4739.55Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
Match NameE-valueIdentityDescription
A0A6J1GLZ41.8e-19098.80mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=... [more]
A0A6J1HZ769.4e-18495.50mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=36... [more]
A0A0A0K6U14.4e-14978.98Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G043... [more]
A0A5A7T9T36.4e-14878.40Mitogen-activated protein kinase kinase kinase 2 OS=Cucumis melo var. makuwa OX=... [more]
A0A1S3C1N14.1e-14778.11mitogen-activated protein kinase kinase kinase 2 OS=Cucumis melo OX=3656 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT1G05100.11.4e-8349.56mitogen-activated protein kinase kinase kinase 18 [more]
AT2G32510.11.4e-8348.15mitogen-activated protein kinase kinase kinase 17 [more]
AT5G55090.11.2e-8247.14mitogen-activated protein kinase kinase kinase 15 [more]
AT4G26890.11.1e-8047.37mitogen-activated protein kinase kinase kinase 16 [more]
AT1G07150.11.6e-5539.60mitogen-activated protein kinase kinase kinase 13 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 3..253
e-value: 2.1E-79
score: 279.8
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 5..253
e-value: 3.0E-59
score: 200.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 3..253
score: 45.304428
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 2..301
e-value: 2.7E-73
score: 249.0
NoneNo IPR availablePANTHERPTHR48011:SF6MAP KINASE KINASE KINASEcoord: 1..328
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 1..328
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 3..253
e-value: 6.49918E-130
score: 369.542
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 9..32
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 121..133
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 4..284

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg19165-RACarg19165-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity