Carg18842 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18842
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein RALF-like 33
Location	Carg_Chr08: 7483300 .. 7483683 (+)
RNA-Seq Expression	Carg18842
Synteny	Carg18842

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATCTCACGCGTTTTTTCCTTCCTCGCGGCCTCCGCCATTATCGCCGCTCACTTTCTGCTCTCATCGGCGATGGCCGTCGATTTCTCCGGCGGTGACAAACTCCTCTTCGTTCCGGCTGAGTCCAAGTGCAGAGGCTCTATCGCTGAATGCTTTCTCGCCGGAGATGACAATGATTCCGCCTTCGGAATGGAGTTCGAGATGGACTCTGAGATCAATCGCCGAATTCTAGCGGCTTCTCGCTACCTGAGCTATGGCGCTTTGAAAAGGAACAGCGTTCCTTGCTCTCGCCGCGGTGCTTCTTACTACAACTGCCAGCCTGGCGCTCAGGCCAATCCTTACAGACGCGGTTGCAACGCCATTACTCGCTGCCGGAGTTAA

mRNA sequence

ATGGAAATCTCACGCGTTTTTTCCTTCCTCGCGGCCTCCGCCATTATCGCCGCTCACTTTCTGCTCTCATCGGCGATGGCCGTCGATTTCTCCGGCGGTGACAAACTCCTCTTCGTTCCGGCTGAGTCCAAGTGCAGAGGCTCTATCGCTGAATGCTTTCTCGCCGGAGATGACAATGATTCCGCCTTCGGAATGGAGTTCGAGATGGACTCTGAGATCAATCGCCGAATTCTAGCGGCTTCTCGCTACCTGAGCTATGGCGCTTTGAAAAGGAACAGCGTTCCTTGCTCTCGCCGCGGTGCTTCTTACTACAACTGCCAGCCTGGCGCTCAGGCCAATCCTTACAGACGCGGTTGCAACGCCATTACTCGCTGCCGGAGTTAA

Coding sequence (CDS)

ATGGAAATCTCACGCGTTTTTTCCTTCCTCGCGGCCTCCGCCATTATCGCCGCTCACTTTCTGCTCTCATCGGCGATGGCCGTCGATTTCTCCGGCGGTGACAAACTCCTCTTCGTTCCGGCTGAGTCCAAGTGCAGAGGCTCTATCGCTGAATGCTTTCTCGCCGGAGATGACAATGATTCCGCCTTCGGAATGGAGTTCGAGATGGACTCTGAGATCAATCGCCGAATTCTAGCGGCTTCTCGCTACCTGAGCTATGGCGCTTTGAAAAGGAACAGCGTTCCTTGCTCTCGCCGCGGTGCTTCTTACTACAACTGCCAGCCTGGCGCTCAGGCCAATCCTTACAGACGCGGTTGCAACGCCATTACTCGCTGCCGGAGTTAA

Protein sequence

MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDNDSAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCNAITRCRS

Homology

[Close]

BLAST of Carg18842 vs. NCBI nr
Match: KAG6593968.1 (Protein RALF-like 33, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026310.1 Protein RALF-like 33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 251.9 bits (642), Expect = 2.9e-63
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 0


Query: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60
           MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND
Sbjct: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60

Query: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120
           SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN
Sbjct: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of Carg18842 vs. NCBI nr
Match: XP_022930438.1 (protein RALF-like 33 [Cucurbita moschata] >XP_023514647.1 protein RALF-like 33 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 250.8 bits (639), Expect = 6.5e-63
Identity = 126/127 (99.21%), Postives = 127/127 (100.00%), Query Frame = 0


Query: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60
           MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND
Sbjct: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60

Query: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120
           SAFGMEFEMDSEINRRILA+SRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN
Sbjct: 61  SAFGMEFEMDSEINRRILASSRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of Carg18842 vs. NCBI nr
Match: XP_023000591.1 (protein RALF-like 33 [Cucurbita maxima])

HSP 1 Score: 247.7 bits (631), Expect = 5.5e-62
Identity = 125/127 (98.43%), Postives = 125/127 (98.43%), Query Frame = 0


Query: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60
           MEISRVFSFLAASAIIAAHFLLSSAM VDFSG DKLLFVPAESKCRGSIAECFLAGDDND
Sbjct: 1   MEISRVFSFLAASAIIAAHFLLSSAMVVDFSGDDKLLFVPAESKCRGSIAECFLAGDDND 60

Query: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120
           SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN
Sbjct: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of Carg18842 vs. NCBI nr
Match: XP_038875155.1 (protein RALF-like 33 [Benincasa hispida])

HSP 1 Score: 211.5 bits (537), Expect = 4.4e-51
Identity = 105/127 (82.68%), Postives = 116/127 (91.34%), Query Frame = 0


Query: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60
           M ISRV S LAA AI+AAHFL+SSA AVDFSG  KLLFVP +S+CRGSIAECFLAG+D+D
Sbjct: 1   MGISRVSSLLAAVAILAAHFLISSATAVDFSGDHKLLFVPTKSECRGSIAECFLAGNDDD 60

Query: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120
           SAFGMEF MDSEINRRILA +RY+SYGAL+RN+VPCSRRGASYYNC+PGAQANPY RGCN
Sbjct: 61  SAFGMEFVMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCN 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of Carg18842 vs. NCBI nr
Match: XP_022138421.1 (protein RALF-like 33 [Momordica charantia])

HSP 1 Score: 203.0 bits (515), Expect = 1.6e-48
Identity = 105/127 (82.68%), Postives = 110/127 (86.61%), Query Frame = 0


Query: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60
           MEISRV S LAA AI AAH LLSSA A DFSG  KLL+VPA+S+CRGSIAEC LAGD  D
Sbjct: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAGD--D 60

Query: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120
           S FG EFEMDSEINRRILA  RY+SYGALKRNSVPCSRRGASYYNCQPGAQANPY RGC+
Sbjct: 61  SGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCS 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 125

[Close]

BLAST of Carg18842 vs. ExPASy Swiss-Prot
Match: Q8L9P8 (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 6.2e-32
Identity = 73/113 (64.60%), Postives = 84/113 (74.34%), Query Frame = 0


Query: 14  AIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDNDSAFGMEFEMDSEI 73
           AI+  HFL   A     S GD   FVP ESKC G+IAEC L+  +       EFEMDSEI
Sbjct: 14  AILTVHFLF--AAVTSQSSGD---FVPIESKCNGTIAECSLSTAEE------EFEMDSEI 73

Query: 74  NRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCNAITRCR 127
           NRRILA ++Y+SYGAL+RN+VPCSRRGASYYNC+ GAQANPY RGC+AITRCR
Sbjct: 74  NRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 115

[Close]

BLAST of Carg18842 vs. ExPASy Swiss-Prot
Match: Q9LUS7 (Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 7.5e-30
Identity = 72/129 (55.81%), Postives = 90/129 (69.77%), Query Frame = 0


Query: 8   SFLAASAIIAAH--FLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLA---GDDND-- 67
           +  A   I+A H   +  S+ + +F+G     F P E++CRG+IAEC ++   GD  D  
Sbjct: 12  AIFAILLILAVHNWSVAVSSQSTEFAGD----FPPFETECRGTIAECSVSAALGDGGDLF 71

Query: 68  ---SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRR 127
                 G EFEMDSEINRRILA  RY+SYGAL+RN++PCSRRGASYYNC+ GAQANPY R
Sbjct: 72  YGGGEMGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSR 131

[Close]

BLAST of Carg18842 vs. ExPASy Swiss-Prot
Match: Q945T0 (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.1e-28
Identity = 63/113 (55.75%), Postives = 78/113 (69.03%), Query Frame = 0


Query: 15  IIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDNDSAFGMEFEMDSEIN 74
           ++   F +S A A D    D ++   +   C+GSI EC    +        EFE+DSE N
Sbjct: 11  VLIGAFFISMAAAGDSGAYDWVMPARSGGGCKGSIGECIAEEE--------EFELDSESN 70

Query: 75  RRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCNAITRCRS 128
           RRILA  +Y+SYGAL++NSVPCSRRGASYYNC+PGAQANPY RGC+AITRCRS
Sbjct: 71  RRILATKKYISYGALQKNSVPCSRRGASYYNCKPGAQANPYSRGCSAITRCRS 115

[Close]

BLAST of Carg18842 vs. ExPASy Swiss-Prot
Match: Q9MA62 (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 6.6e-26
Identity = 67/121 (55.37%), Postives = 79/121 (65.29%), Query Frame = 0


Query: 11  AASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESK-----CRGSIAECFLAGDDNDSAFGM 70
           A  A+IA   ++ SA+      GD L FV A S      C GSIAEC    +        
Sbjct: 6   AIYAVIAILAIVISAVESTGDFGDSLDFVRAGSSSLFSGCTGSIAECIAEEE-------- 65

Query: 71  EFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCNAITRC 127
           E E DS+I+RRILA  +Y+SYGA++RNSVPCSRRGASYYNCQ GAQANPY RGC+ ITRC
Sbjct: 66  EMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRC 118

[Close]

BLAST of Carg18842 vs. ExPASy Swiss-Prot
Match: Q9SRY3 (Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 3.3e-25
Identity = 58/85 (68.24%), Postives = 65/85 (76.47%), Query Frame = 0


Query: 43  SKCRGSIAECFLAGDDNDSAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGAS 102
           S C GSIAEC  A ++         EMDSEINRRILA ++Y+SY +LKRNSVPCSRRGAS
Sbjct: 45  SGCHGSIAECIGAEEE---------EMDSEINRRILATTKYISYQSLKRNSVPCSRRGAS 104

Query: 103 YYNCQPGAQANPYRRGCNAITRCRS 128
           YYNCQ GAQANPY RGC+ I RCRS
Sbjct: 105 YYNCQNGAQANPYSRGCSKIARCRS 120

[Close]

BLAST of Carg18842 vs. ExPASy TrEMBL
Match: A0A6J1EV64 (protein RALF-like 33 OS=Cucurbita moschata OX=3662 GN=LOC111436882 PE=3 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.1e-63
Identity = 126/127 (99.21%), Postives = 127/127 (100.00%), Query Frame = 0


Query: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60
           MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND
Sbjct: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60

Query: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120
           SAFGMEFEMDSEINRRILA+SRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN
Sbjct: 61  SAFGMEFEMDSEINRRILASSRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of Carg18842 vs. ExPASy TrEMBL
Match: A0A6J1KG89 (protein RALF-like 33 OS=Cucurbita maxima OX=3661 GN=LOC111494831 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.7e-62
Identity = 125/127 (98.43%), Postives = 125/127 (98.43%), Query Frame = 0


Query: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60
           MEISRVFSFLAASAIIAAHFLLSSAM VDFSG DKLLFVPAESKCRGSIAECFLAGDDND
Sbjct: 1   MEISRVFSFLAASAIIAAHFLLSSAMVVDFSGDDKLLFVPAESKCRGSIAECFLAGDDND 60

Query: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120
           SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN
Sbjct: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of Carg18842 vs. ExPASy TrEMBL
Match: A0A6J1C9N4 (protein RALF-like 33 OS=Momordica charantia OX=3673 GN=LOC111009595 PE=3 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 7.5e-49
Identity = 105/127 (82.68%), Postives = 110/127 (86.61%), Query Frame = 0


Query: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60
           MEISRV S LAA AI AAH LLSSA A DFSG  KLL+VPA+S+CRGSIAEC LAGD  D
Sbjct: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAGD--D 60

Query: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120
           S FG EFEMDSEINRRILA  RY+SYGALKRNSVPCSRRGASYYNCQPGAQANPY RGC+
Sbjct: 61  SGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCS 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 125

[Close]

BLAST of Carg18842 vs. ExPASy TrEMBL
Match: A0A6J1H3W7 (protein RALF-like 33 OS=Cucurbita moschata OX=3662 GN=LOC111460267 PE=3 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 9.8e-49
Identity = 96/127 (75.59%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60
           M ISRV + +AA A++AAHFL+ SA A+DFSG +++LF P +S+CRGSIAECFLAG+D+D
Sbjct: 1   MGISRVSALIAAFAVLAAHFLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDD 60

Query: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120
           S FGMEFEMDSEINRRILA +RY+SYGAL+RN+VPCSRRGASYYNC+PGAQANPY RGC+
Sbjct: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of Carg18842 vs. ExPASy TrEMBL
Match: A0A6J1KVY0 (protein RALF-like 33 OS=Cucurbita maxima OX=3661 GN=LOC111499183 PE=3 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 6.4e-48
Identity = 95/127 (74.80%), Postives = 114/127 (89.76%), Query Frame = 0


Query: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60
           M ISRV + +AA A++AAHFL+SSA A+DFSG +++LF P +S+CRGSI ECFLAG+ +D
Sbjct: 1   MGISRVSALIAAFAVLAAHFLISSATAIDFSGDNEILFAPTKSECRGSITECFLAGNYDD 60

Query: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120
           S FGMEFEMDSEINRRILA +RY+SYGAL+RN+VPCSRRGASYYNC+PGAQANPY RGC+
Sbjct: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of Carg18842 vs. TAIR 10
Match: AT4G15800.1 (ralf-like 33 )

HSP 1 Score: 138.3 bits (347), Expect = 4.4e-33
Identity = 73/113 (64.60%), Postives = 84/113 (74.34%), Query Frame = 0


Query: 14  AIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDNDSAFGMEFEMDSEI 73
           AI+  HFL   A     S GD   FVP ESKC G+IAEC L+  +       EFEMDSEI
Sbjct: 14  AILTVHFLF--AAVTSQSSGD---FVPIESKCNGTIAECSLSTAEE------EFEMDSEI 73

Query: 74  NRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCNAITRCR 127
           NRRILA ++Y+SYGAL+RN+VPCSRRGASYYNC+ GAQANPY RGC+AITRCR
Sbjct: 74  NRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 115

[Close]

BLAST of Carg18842 vs. TAIR 10
Match: AT3G16570.1 (rapid alkalinization factor 23 )

HSP 1 Score: 131.3 bits (329), Expect = 5.3e-31
Identity = 72/129 (55.81%), Postives = 90/129 (69.77%), Query Frame = 0


Query: 8   SFLAASAIIAAH--FLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLA---GDDND-- 67
           +  A   I+A H   +  S+ + +F+G     F P E++CRG+IAEC ++   GD  D  
Sbjct: 12  AIFAILLILAVHNWSVAVSSQSTEFAGD----FPPFETECRGTIAECSVSAALGDGGDLF 71

Query: 68  ---SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRR 127
                 G EFEMDSEINRRILA  RY+SYGAL+RN++PCSRRGASYYNC+ GAQANPY R
Sbjct: 72  YGGGEMGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSR 131

[Close]

BLAST of Carg18842 vs. TAIR 10
Match: AT3G05490.1 (ralf-like 22 )

HSP 1 Score: 118.2 bits (295), Expect = 4.7e-27
Identity = 67/121 (55.37%), Postives = 79/121 (65.29%), Query Frame = 0


Query: 11  AASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESK-----CRGSIAECFLAGDDNDSAFGM 70
           A  A+IA   ++ SA+      GD L FV A S      C GSIAEC    +        
Sbjct: 6   AIYAVIAILAIVISAVESTGDFGDSLDFVRAGSSSLFSGCTGSIAECIAEEE-------- 65

Query: 71  EFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCNAITRC 127
           E E DS+I+RRILA  +Y+SYGA++RNSVPCSRRGASYYNCQ GAQANPY RGC+ ITRC
Sbjct: 66  EMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRC 118

[Close]

BLAST of Carg18842 vs. TAIR 10
Match: AT1G02900.1 (rapid alkalinization factor 1 )

HSP 1 Score: 115.9 bits (289), Expect = 2.3e-26
Identity = 58/85 (68.24%), Postives = 65/85 (76.47%), Query Frame = 0


Query: 43  SKCRGSIAECFLAGDDNDSAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGAS 102
           S C GSIAEC  A ++         EMDSEINRRILA ++Y+SY +LKRNSVPCSRRGAS
Sbjct: 45  SGCHGSIAECIGAEEE---------EMDSEINRRILATTKYISYQSLKRNSVPCSRRGAS 104

Query: 103 YYNCQPGAQANPYRRGCNAITRCRS 128
           YYNCQ GAQANPY RGC+ I RCRS
Sbjct: 105 YYNCQNGAQANPYSRGCSKIARCRS 120

[Close]

BLAST of Carg18842 vs. TAIR 10
Match: AT2G33775.1 (ralf-like 19 )

HSP 1 Score: 87.0 bits (214), Expect = 1.2e-17
Identity = 42/71 (59.15%), Postives = 55/71 (77.46%), Query Frame = 0


Query: 56  GDDNDSAFGMEFEMDSEINRRILAASR-YLSYGALKRNSVPCSRRGASYYNCQPGAQANP 115
           G+D +    +++ MDSE NRR LAA R Y+SYGAL++N+VPCSRRG SYY+C+   +ANP
Sbjct: 39  GEDGE----LDYLMDSETNRRQLAARRSYISYGALRKNNVPCSRRGRSYYDCKKRKRANP 98

Query: 116 YRRGCNAITRC 126
           YRRGC+ IT C
Sbjct: 99  YRRGCSVITHC 105

The following BLAST results are available for this feature:

BLAST of Carg18842 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6593968.1	2.9e-63	100.00	Protein RALF-like 33, partial [Cucurbita argyrosperma subsp. sororia] >KAG702631...	[more]
XP_022930438.1	6.5e-63	99.21	protein RALF-like 33 [Cucurbita moschata] >XP_023514647.1 protein RALF-like 33 [...	[more]
XP_023000591.1	5.5e-62	98.43	protein RALF-like 33 [Cucurbita maxima]	[more]
XP_038875155.1	4.4e-51	82.68	protein RALF-like 33 [Benincasa hispida]	[more]
XP_022138421.1	1.6e-48	82.68	protein RALF-like 33 [Momordica charantia]	[more]

BLAST of Carg18842 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L9P8	6.2e-32	64.60	Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1	[more]
Q9LUS7	7.5e-30	55.81	Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV...	[more]
Q945T0	4.1e-28	55.75	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]
Q9MA62	6.6e-26	55.37	Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1	[more]
Q9SRY3	3.3e-25	68.24	Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1	[more]

BLAST of Carg18842 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EV64	3.1e-63	99.21	protein RALF-like 33 OS=Cucurbita moschata OX=3662 GN=LOC111436882 PE=3 SV=1	[more]
A0A6J1KG89	2.7e-62	98.43	protein RALF-like 33 OS=Cucurbita maxima OX=3661 GN=LOC111494831 PE=3 SV=1	[more]
A0A6J1C9N4	7.5e-49	82.68	protein RALF-like 33 OS=Momordica charantia OX=3673 GN=LOC111009595 PE=3 SV=1	[more]
A0A6J1H3W7	9.8e-49	75.59	protein RALF-like 33 OS=Cucurbita moschata OX=3662 GN=LOC111460267 PE=3 SV=1	[more]
A0A6J1KVY0	6.4e-48	74.80	protein RALF-like 33 OS=Cucurbita maxima OX=3661 GN=LOC111499183 PE=3 SV=1	[more]

BLAST of Carg18842 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15800.1	4.4e-33	64.60	ralf-like 33	[more]
AT3G16570.1	5.3e-31	55.81	rapid alkalinization factor 23	[more]
AT3G05490.1	4.7e-27	55.37	ralf-like 22	[more]
AT1G02900.1	2.3e-26	68.24	rapid alkalinization factor 1	[more]
AT2G33775.1	1.2e-17	59.15	ralf-like 19	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 120..127
None	No IPR available	PANTHER	PTHR33136:SF50	PROTEIN RALF-LIKE 33	coord: 9..127
None	No IPR available	PANTHER	PTHR33136	RAPID ALKALINIZATION FACTOR-LIKE	coord: 9..127
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 66..126 e-value: 1.3E-29 score: 102.9

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18842-RA	Carg18842-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019722	calcium-mediated signaling
cellular_component	GO:0005576	extracellular region