Carg18681 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18681
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type E3 ubiquitin transferase
Location	Carg_Chr17: 7462254 .. 7463480 (-)
RNA-Seq Expression	Carg18681
Synteny	Carg18681

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTGTAATTTACGTATATTATCCATGAAGTAGTTTGTGGACTTTCTCACATGATGATGCTTGTTTCTCGAATTTTTTAGCATTTCTACTTCCCTTGTTACATGCATCTGCATTGTACTCAGCTATATTAACTTCAAAATGAGATTCAAAATTTGATGTGATTCTCATTGCAGCACGCTCATTGGGATGGCCGGTTGGAAGAGATCACTCTGCGTCATATTGGGAATCAAGATGGTCTCACTGTTATCGCCAAAAATATTAGGTTTTGTTTTGTGGATTTGCTTCTTGTTGTCTATTTTTGTTCAACGTTACCTTTTATAAATTTGAAGCTATTTTCAGGTTGTCGGAATTTCTCAGTGACTTGCATATCAGGTGAAGAATCATTCCATCAGGTATGAGAACACACTAAAGACTCCTCACCCCTATAGTTCACCATCATGCCAATATGATGTCCACTACGTAATATGTCAGATTTTAATATGATCATTATTAATTTTATATTGCTAGTCGTTTCCTACTATCATAAGGTTTTGTTTGGACTCCGTAATTCAGTACGTAATATGTCAGATTTTAATATGATCATTATTAATTTTATATTGCTAGTCGTTTCCTACTATCATAAGGTTTTGTTTGGACTTCGTAAGTCAGTATCTTTATCAATTGAAACCCAGCATATTTTATTCGAAACGGAGTCGATTAATGTTGGCACAATCTGTTATCACTATATTTCAATGCCAAATTTTATGTATTGTATTAGTCTTCAATTACACTTGCAATATCATCTATGAGTTTGAAAATCAGTTTCAGATGCGCTATTGCCTGAAATTAACAATGTAGCCCATGAAATGCCAACCTTTGTTTATTATTAGTCTTCATTTACACTTGCAATGTAATTGAAGAGTTTGAAAACCAGTTTCAGATGTTGAATTGCTTGCTGGAGTCTGATATTGAGAAACGAAAGGTGGGTTACCGAGCACGGTTCTTCGAACTTCATCTTCTATGTATCAAAATGAAGCTGGAAGACATTATGTCATACTGAGGAATGAAGGCAGGTGAATAAATTGTAAGGGTTCTACGCTGTTGTTGTTGTTGTACTAAACATTGGAAAATGACCGAATGTATTTGTTTGTATTTTAAGAAGAAGATAAATGGGGATGTCGATGTCGATGTCGATGTCGTGTGTGGTTTGATAGAGAAACAAGAGAAAAGGAAACTTCAAGGCTAG

mRNA sequence

ATGCCTCACGCTCATTGGGATGGCCGGTTGGAAGAGATCACTCTGCGTCATATTGGGAATCAAGATGGTCTCACTGTTATCGCCAAAAATATTAGGTTTTGTTTTGTGGATTTGCTTCTTGTTGTCTATTTTTGTTCAACGTTACCTTTTATAAATTTGAAGCTATTTTCAGGTTGTCGGAATTTCTCAGTGACTTGCATATCAGGTGAAGAATCATTCCATCAGAAGAAGATAAATGGGGATGTCGATGTCGATGTCGATGTCGTGTGTGGTTTGATAGAGAAACAAGAGAAAAGGAAACTTCAAGGCTAG

Coding sequence (CDS)

ATGCCTCACGCTCATTGGGATGGCCGGTTGGAAGAGATCACTCTGCGTCATATTGGGAATCAAGATGGTCTCACTGTTATCGCCAAAAATATTAGGTTTTGTTTTGTGGATTTGCTTCTTGTTGTCTATTTTTGTTCAACGTTACCTTTTATAAATTTGAAGCTATTTTCAGGTTGTCGGAATTTCTCAGTGACTTGCATATCAGGTGAAGAATCATTCCATCAGAAGAAGATAAATGGGGATGTCGATGTCGATGTCGATGTCGTGTGTGGTTTGATAGAGAAACAAGAGAAAAGGAAACTTCAAGGCTAG

Protein sequence

MPHAHWDGRLEEITLRHIGNQDGLTVIAKNIRFCFVDLLLVVYFCSTLPFINLKLFSGCRNFSVTCISGEESFHQKKINGDVDVDVDVVCGLIEKQEKRKLQG

Homology

[Close]

BLAST of Carg18681 vs. NCBI nr
Match: KAG7014236.1 (hypothetical protein SDJN02_24411, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 217.6 bits (553), Expect = 4.9e-53
Identity = 103/103 (100.00%), Postives = 103/103 (100.00%), Query Frame = 0


Query: 1   MPHAHWDGRLEEITLRHIGNQDGLTVIAKNIRFCFVDLLLVVYFCSTLPFINLKLFSGCR 60
           MPHAHWDGRLEEITLRHIGNQDGLTVIAKNIRFCFVDLLLVVYFCSTLPFINLKLFSGCR
Sbjct: 1   MPHAHWDGRLEEITLRHIGNQDGLTVIAKNIRFCFVDLLLVVYFCSTLPFINLKLFSGCR 60

Query: 61  NFSVTCISGEESFHQKKINGDVDVDVDVVCGLIEKQEKRKLQG 104
           NFSVTCISGEESFHQKKINGDVDVDVDVVCGLIEKQEKRKLQG
Sbjct: 61  NFSVTCISGEESFHQKKINGDVDVDVDVVCGLIEKQEKRKLQG 103

[Close]

BLAST of Carg18681 vs. NCBI nr
Match: XP_023547952.1 (E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023547953.1 E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 68.6 bits (166), Expect = 3.7e-08
Identity = 30/30 (100.00%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 3   HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 33
           HAHWDGRLEEITLRHIGNQDGLTVIAKNIR
Sbjct: 319 HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 348

[Close]

BLAST of Carg18681 vs. NCBI nr
Match: XP_022954068.1 (E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita moschata] >XP_022954069.1 E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 68.6 bits (166), Expect = 3.7e-08
Identity = 30/30 (100.00%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 3   HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 33
           HAHWDGRLEEITLRHIGNQDGLTVIAKNIR
Sbjct: 319 HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 348

[Close]

BLAST of Carg18681 vs. NCBI nr
Match: XP_022991900.1 (E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita maxima] >XP_022991901.1 E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 68.6 bits (166), Expect = 3.7e-08
Identity = 30/30 (100.00%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 3   HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 33
           HAHWDGRLEEITLRHIGNQDGLTVIAKNIR
Sbjct: 319 HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 348

[Close]

BLAST of Carg18681 vs. NCBI nr
Match: XP_028952684.1 (putative RING-type E3 ubiquitin transferase C3H69 isoform X2 [Malus domestica])

HSP 1 Score: 65.1 bits (157), Expect = 4.1e-07
Identity = 36/66 (54.55%), Postives = 45/66 (68.18%), Query Frame = 0


Query: 3   HAHWDGRLEEITLRHIGNQDGLTVIAKNIRFCFVDLLL-VVYFCSTLPF-INLKLFSGCR 62
           HA+ DGRLEE+ LRHIGN+DG TVIA NIRF  V L++  +YF    PF     +FSGC+
Sbjct: 322 HAYRDGRLEEVALRHIGNEDGQTVIATNIRFYPVPLIISSIYF----PFHTKSVMFSGCQ 381

Query: 63  NFSVTC 67
            F + C
Sbjct: 382 TFCLIC 383

[Close]

BLAST of Carg18681 vs. ExPASy Swiss-Prot
Match: Q5ZA07 (E3 ubiquitin-protein ligase makorin OS=Oryza sativa subsp. japonica OX=39947 GN=MKRN PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.2e-04
Identity = 21/30 (70.00%), Postives = 24/30 (80.00%), Query Frame = 0


Query: 3   HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 33
           HA+ DGRLEE+ LRH+   DG TVIAKNIR
Sbjct: 329 HAYRDGRLEEVILRHLDADDGSTVIAKNIR 358

[Close]

BLAST of Carg18681 vs. ExPASy Swiss-Prot
Match: E0X9N4 (Putative RING-type E3 ubiquitin transferase C3H69 OS=Arabidopsis thaliana OX=3702 GN=At3g63550 PE=3 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.5e-04
Identity = 20/30 (66.67%), Postives = 25/30 (83.33%), Query Frame = 0


Query: 3   HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 33
           HA+ DGRLEE+ LRH+  +DG TVIAK+IR
Sbjct: 311 HAYHDGRLEEVVLRHLDAEDGQTVIAKDIR 340

[Close]

BLAST of Carg18681 vs. ExPASy TrEMBL
Match: A0A6J1JU79 (E3 ubiquitin-protein ligase makorin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488400 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.8e-08
Identity = 30/30 (100.00%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 3   HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 33
           HAHWDGRLEEITLRHIGNQDGLTVIAKNIR
Sbjct: 319 HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 348

[Close]

BLAST of Carg18681 vs. ExPASy TrEMBL
Match: A0A6J1GRT6 (E3 ubiquitin-protein ligase makorin-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456442 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.8e-08
Identity = 30/30 (100.00%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 3   HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 33
           HAHWDGRLEEITLRHIGNQDGLTVIAKNIR
Sbjct: 319 HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 348

[Close]

BLAST of Carg18681 vs. ExPASy TrEMBL
Match: A0A6J1KWK8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111497821 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 4.9e-06
Identity = 26/30 (86.67%), Postives = 28/30 (93.33%), Query Frame = 0


Query: 3   HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 33
           HAH DGRLEE+ LRHIGNQDGLT+IAKNIR
Sbjct: 334 HAHRDGRLEEVALRHIGNQDGLTIIAKNIR 363

[Close]

BLAST of Carg18681 vs. ExPASy TrEMBL
Match: A0A6J1KUX3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111497821 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 4.9e-06
Identity = 26/30 (86.67%), Postives = 28/30 (93.33%), Query Frame = 0


Query: 3   HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 33
           HAH DGRLEE+ LRHIGNQDGLT+IAKNIR
Sbjct: 319 HAHRDGRLEEVALRHIGNQDGLTIIAKNIR 348

[Close]

BLAST of Carg18681 vs. ExPASy TrEMBL
Match: A0A4S4DC59 (RING-type E3 ubiquitin transferase OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_003366 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 4.9e-06
Identity = 37/102 (36.27%), Postives = 54/102 (52.94%), Query Frame = 0


Query: 3   HAHWDGRLEEITLRHIGNQDGLTVIAKNIRFCFVDLLLVVYFCSTLPFINLK-LFSGCRN 62
           HA+ DGRLEE+ LRH+G +DG TVIAKNIRFC +  +L  +      F+ +  LF     
Sbjct: 513 HAYRDGRLEEVVLRHLGAEDGHTVIAKNIRFCLLPCILPSFHLLATTFVVISFLFVSLSR 572

Query: 63  FSVTCIS-GEESFHQKKINGDVDVDVDVVCGLIEKQEKRKLQ 103
           F     + G       K+N   ++   V  G+++   +RK Q
Sbjct: 573 FDAYFQALGLPQKFAHKVNACFELPGIVYQGVVKGAPRRKAQ 614

[Close]

BLAST of Carg18681 vs. TAIR 10
Match: AT3G08505.2 (zinc finger (CCCH-type/C3HC4-type RING finger) family protein )

HSP 1 Score: 43.9 bits (102), Expect = 9.1e-05
Identity = 18/30 (60.00%), Postives = 24/30 (80.00%), Query Frame = 0


Query: 3   HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 33
           HA+ DG LEE+ LRH+G+Q+G TVI  +IR
Sbjct: 283 HAYSDGHLEEVVLRHLGSQEGETVITDSIR 312

[Close]

BLAST of Carg18681 vs. TAIR 10
Match: AT3G08505.1 (zinc finger (CCCH-type/C3HC4-type RING finger) family protein )

HSP 1 Score: 43.9 bits (102), Expect = 9.1e-05
Identity = 18/30 (60.00%), Postives = 24/30 (80.00%), Query Frame = 0


Query: 3   HAHWDGRLEEITLRHIGNQDGLTVIAKNIR 33
           HA+ DG LEE+ LRH+G+Q+G TVI  +IR
Sbjct: 283 HAYSDGHLEEVVLRHLGSQEGETVITDSIR 312

The following BLAST results are available for this feature:

BLAST of Carg18681 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014236.1	4.9e-53	100.00	hypothetical protein SDJN02_24411, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023547952.1	3.7e-08	100.00	E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita pepo subsp. pepo]...	[more]
XP_022954068.1	3.7e-08	100.00	E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita moschata] >XP_022...	[more]
XP_022991900.1	3.7e-08	100.00	E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita maxima] >XP_02299...	[more]
XP_028952684.1	4.1e-07	54.55	putative RING-type E3 ubiquitin transferase C3H69 isoform X2 [Malus domestica]	[more]

BLAST of Carg18681 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5ZA07	1.2e-04	70.00	E3 ubiquitin-protein ligase makorin OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
E0X9N4	1.5e-04	66.67	Putative RING-type E3 ubiquitin transferase C3H69 OS=Arabidopsis thaliana OX=370...	[more]

BLAST of Carg18681 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JU79	1.8e-08	100.00	E3 ubiquitin-protein ligase makorin-like isoform X2 OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1GRT6	1.8e-08	100.00	E3 ubiquitin-protein ligase makorin-like isoform X2 OS=Cucurbita moschata OX=366...	[more]
A0A6J1KWK8	4.9e-06	86.67	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111497821 P...	[more]
A0A6J1KUX3	4.9e-06	86.67	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111497821 P...	[more]
A0A4S4DC59	4.9e-06	36.27	RING-type E3 ubiquitin transferase OS=Camellia sinensis var. sinensis OX=542762 ...	[more]

BLAST of Carg18681 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G08505.2	9.1e-05	60.00	zinc finger (CCCH-type/C3HC4-type RING finger) family protein	[more]
AT3G08505.1	9.1e-05	60.00	zinc finger (CCCH-type/C3HC4-type RING finger) family protein	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18681-RA	Carg18681-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0046872	metal ion binding