Carg18630 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18630
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Cytokinin dehydrogenase
Location	Carg_Chr12: 11890193 .. 11892794 (-)
RNA-Seq Expression	Carg18630
Synteny	Carg18630

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGGAAAATCTTCCGGTTCATTTCATAGTCACTTTCTTCATAAACCGGTTAAAATCCAGCATAAACAAATCCAAGCCATGGATTACCTTACACCCGCAACCAAACAACCTTGTACATGATCCCGAATCTTTAACCTTAGCCTCCTCCGATTACGGGAATATGGTCAAAGAAATCCCCGCTGCGGTTCTTCAACCAACCTCCATAAACGACGTCGTTCACCTAATAAATTATTCCTACAACAACCCTATCCCATTTCCGGTAGCCGCCAGAGGACAAGGCCACTCCGTACGCGGCCAAGCCATGGCAAAAAACGGCGTCGTAATCGACATGTCGTATTTGCGGCGGAATCGGGAAACCCCTGGAATTATAATCTCCCGCCTCCGCTCCACCGGGGAGATTTACGCCGACGTCGGCGGAGAGCAGCTGTGGATTGACGTGTTGAATGCGGCGTTGGCGTATGGGGTGACGCCGGTGTCGTGGACGGATTATTTGTACATCACGGTGGGGGGGACGTTATCAAATGGCGGGATAAGCGGGCAGAGTTTCCGGTACGGGCCGCAAGTGAGCAATGTGGTGGAGATGGATGTCGTCACAGGTATGGAAAACGAAACGACGTGTAATTTTGTATTTAAATATAAGTATAAATAAATAATAATAATTTATTTGTTCATACCATATTAAAAGGACAAGTTTGGTTGTTACATAATGTGTATTAAGTACAGACATTTTTCTACATATCATTAAATGATATGAGAAAGCTGTTTATTTTGTGGTCCCTAAGTTTGAACTCTAAAAAAAAAATGTATTTTTCAAACTAGGTGTTTCCAGTCTATTTTGTACAAATAGACAGCTTGTCTTTTAAAAATAAATTATTATTGCATGCATGCAATTAATGATAACATGACTCTTGACTCTCGGTATGATATTGTCCACTTCAAGCATAAGCTCTTATGGCTTTATTTAGGACATACCTAAAAGGATTCATCATATCAATGAATATAGTATTTTTTTATTATAAACTCAGGATCATTTTCTAAATTTGCAGGTAAGGGGAATATGGTGACTTGCTCTACTAAAAAGAATTCCGAGCTATTCCATGCGGTCCTCGGAGGTTTGGGTCAATTTGGGATTATTGCTCGAGCAAGAATAGCTTTGGAACCAGCTCCAAGGAGGGTAATTTTTATTTATTTTATTTTATTTTATGGTTTTCTTATAAAATATTTAAAAGGCTTGTTCTTATAATAATGGTTTATATTTCTTTGATATATATATATATATAGTAAGGTTTTATATAGTTTGTAAGGTTGATTAAAAGTAAGGAAGAATCTATGGATTATTCAATGGATGCTTCTAAACCACCCAACATATCATCGTCTTTGTTTGGGAGTGTTTCTGTCATTTCCAACCATCAAAACGGTGTCGTATCTAATAATACGAACATATTCCAAATTAATTCTCACGCATATGTTTTACTATTTCTTAATCTCATGCTTTGTCTGCTAATTATGATTATTATTATTATTACTATTATGTACGCACGCATGTGAAATTCGAAGATACTGATGATGATGTGGAATTCGGTCGCAGGTTAAATGGATACGAATGTTGTACACTAACTTTTCCGCGTTTGCCAAAGACCAAGAGCGTTTGATCTCATTCAATGAAAGACAACAAGCGAATGCGCTTGATTATTTGGAGGGTTTGGTTCTGCTGCATCATGACTCACCGGATACTTGGCGTTCCTCGTTCTTTCCACCGTCTGATCATCCTAGAATAATCTCTTTGGCTAATCAAAACTCCCTTATTTATTGCCTCGAGGTTGCCAAATATTATGATCGTCATACTCAGTCTACTATAGACAAGGTATAAATTTAATACTTTTCTCTCTCTCGATGTCTAAAATAAGATAATTCTTATATGTTTGTGTTGTTGTTTTTTTTTTTTTTTGGTAGGATATTGAAGTATTATTCGAAGGGTTGGATTATGAGAGCGGGTTCAAGTTTGAGAAAGATGTAACGTACGTGAAATTTCTAAATAGAGTTAGAAGTGGAGAGCTGAAGCTTCAATCCCAGGGACTATGGAACGTTCCTCATCCATGGCTAAATCTCTTTGTCCCGAAATCTCGTATCTTGGATTTTGATGTAGGTGTCTTTAAAGACATAGTTTTACGACGAAATATAACCAAAGGACCTGTACTCATCTACCCAATGAACCACTCCAAGTAAGCTCGTAGAATAACGTTTTCTATACCTAACGTATATCGATTGATTGTAATTAATATTATGAATTGGTACGATAAAAATGCAGGTGGGATAGTAGAAATTCAACGGTTATACCTGACGAAGAGGTGTTTTACACGATAGGATTTTTGCATTCAAGTGGGTTTGATGACTGGAAATCGTTTGACCGACAAAACGATGAGATATTAGAATTTTGTGAGAAAAGTGGAATTGAAATCAAACAATATCTTCCGAATTACAAGACACAAAGACAATGGATCGACCATTTTGGTTCCAAATGGACAAATTTTCGAGACATGAAAATGAAGTTCGACCCTAAATATATTCTCTCTCCCGGACAGAGAATTTTCAATGTCTGA

mRNA sequence

ATGACGGAAAATCTTCCGGTTCATTTCATAGTCACTTTCTTCATAAACCGGTTAAAATCCAGCATAAACAAATCCAAGCCATGGATTACCTTACACCCGCAACCAAACAACCTTGTACATGATCCCGAATCTTTAACCTTAGCCTCCTCCGATTACGGGAATATGGTCAAAGAAATCCCCGCTGCGGTTCTTCAACCAACCTCCATAAACGACGTCGTTCACCTAATAAATTATTCCTACAACAACCCTATCCCATTTCCGGTAGCCGCCAGAGGACAAGGCCACTCCGTACGCGGCCAAGCCATGGCAAAAAACGGCGTCGTAATCGACATGTCGTATTTGCGGCGGAATCGGGAAACCCCTGGAATTATAATCTCCCGCCTCCGCTCCACCGGGGAGATTTACGCCGACGTCGGCGGAGAGCAGCTGTGGATTGACGTGTTGAATGCGGCGTTGGCGTATGGGGTGACGCCGGTGTCGTGGACGGATTATTTGTACATCACGGTGGGGGGGACGTTATCAAATGGCGGGATAAGCGGGCAGAGTTTCCGGTACGGGCCGCAAGTGAGCAATGTGGTGGAGATGGATGTCGTCACAGCTCCAAGGAGGGTTAAATGGATACGAATGTTGTACACTAACTTTTCCGCGTTTGCCAAAGACCAAGAGCGTTTGATCTCATTCAATGAAAGACAACAAGCGAATGCGCTTGATTATTTGGAGGGTTTGGTTCTGCTGCATCATGACTCACCGGATACTTGGCGTTCCTCGTTCTTTCCACCGTCTGATCATCCTAGAATAATCTCTTTGGCTAATCAAAACTCCCTTATTTATTGCCTCGAGGTTGCCAAATATTATGATCGTCATACTCAGTCTACTATAGACAAGGATATTGAAGTATTATTCGAAGGGTTGGATTATGAGAGCGGGTTCAAGTTTGAGAAAGATGTAACGTACGTGAAATTTCTAAATAGAGTTAGAAGTGGAGAGCTGAAGCTTCAATCCCAGGGACTATGGAACGTTCCTCATCCATGGCTAAATCTCTTTGTCCCGAAATCTCGTATCTTGGATTTTGATGTAGGTGTCTTTAAAGACATAGTTTTACGACGAAATATAACCAAAGGACCTGTACTCATCTACCCAATGAACCACTCCAAGTGGGATAGTAGAAATTCAACGGTTATACCTGACGAAGAGGTGTTTTACACGATAGGATTTTTGCATTCAAGTGGGTTTGATGACTGGAAATCGTTTGACCGACAAAACGATGAGATATTAGAATTTTGTGAGAAAAGTGGAATTGAAATCAAACAATATCTTCCGAATTACAAGACACAAAGACAATGGATCGACCATTTTGGTTCCAAATGGACAAATTTTCGAGACATGAAAATGAAGTTCGACCCTAAATATATTCTCTCTCCCGGACAGAGAATTTTCAATGTCTGA

Coding sequence (CDS)

ATGACGGAAAATCTTCCGGTTCATTTCATAGTCACTTTCTTCATAAACCGGTTAAAATCCAGCATAAACAAATCCAAGCCATGGATTACCTTACACCCGCAACCAAACAACCTTGTACATGATCCCGAATCTTTAACCTTAGCCTCCTCCGATTACGGGAATATGGTCAAAGAAATCCCCGCTGCGGTTCTTCAACCAACCTCCATAAACGACGTCGTTCACCTAATAAATTATTCCTACAACAACCCTATCCCATTTCCGGTAGCCGCCAGAGGACAAGGCCACTCCGTACGCGGCCAAGCCATGGCAAAAAACGGCGTCGTAATCGACATGTCGTATTTGCGGCGGAATCGGGAAACCCCTGGAATTATAATCTCCCGCCTCCGCTCCACCGGGGAGATTTACGCCGACGTCGGCGGAGAGCAGCTGTGGATTGACGTGTTGAATGCGGCGTTGGCGTATGGGGTGACGCCGGTGTCGTGGACGGATTATTTGTACATCACGGTGGGGGGGACGTTATCAAATGGCGGGATAAGCGGGCAGAGTTTCCGGTACGGGCCGCAAGTGAGCAATGTGGTGGAGATGGATGTCGTCACAGCTCCAAGGAGGGTTAAATGGATACGAATGTTGTACACTAACTTTTCCGCGTTTGCCAAAGACCAAGAGCGTTTGATCTCATTCAATGAAAGACAACAAGCGAATGCGCTTGATTATTTGGAGGGTTTGGTTCTGCTGCATCATGACTCACCGGATACTTGGCGTTCCTCGTTCTTTCCACCGTCTGATCATCCTAGAATAATCTCTTTGGCTAATCAAAACTCCCTTATTTATTGCCTCGAGGTTGCCAAATATTATGATCGTCATACTCAGTCTACTATAGACAAGGATATTGAAGTATTATTCGAAGGGTTGGATTATGAGAGCGGGTTCAAGTTTGAGAAAGATGTAACGTACGTGAAATTTCTAAATAGAGTTAGAAGTGGAGAGCTGAAGCTTCAATCCCAGGGACTATGGAACGTTCCTCATCCATGGCTAAATCTCTTTGTCCCGAAATCTCGTATCTTGGATTTTGATGTAGGTGTCTTTAAAGACATAGTTTTACGACGAAATATAACCAAAGGACCTGTACTCATCTACCCAATGAACCACTCCAAGTGGGATAGTAGAAATTCAACGGTTATACCTGACGAAGAGGTGTTTTACACGATAGGATTTTTGCATTCAAGTGGGTTTGATGACTGGAAATCGTTTGACCGACAAAACGATGAGATATTAGAATTTTGTGAGAAAAGTGGAATTGAAATCAAACAATATCTTCCGAATTACAAGACACAAAGACAATGGATCGACCATTTTGGTTCCAAATGGACAAATTTTCGAGACATGAAAATGAAGTTCGACCCTAAATATATTCTCTCTCCCGGACAGAGAATTTTCAATGTCTGA

Protein sequence

MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVTAPRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLEGLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVLFEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVGVFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV

Homology

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BLAST of Carg18630 vs. NCBI nr
Match: KAG7021337.1 (Cytokinin dehydrogenase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1001.1 bits (2587), Expect = 3.2e-288
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0


Query: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60
           MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP
Sbjct: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60

Query: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120
           AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET
Sbjct: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120

Query: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180
           PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG
Sbjct: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180

Query: 181 QSFRYGPQVSNVVEMDVVTAPRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLE 240
           QSFRYGPQVSNVVEMDVVTAPRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLE
Sbjct: 181 QSFRYGPQVSNVVEMDVVTAPRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLE 240

Query: 241 GLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVL 300
           GLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVL
Sbjct: 241 GLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVL 300

Query: 301 FEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVG 360
           FEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVG
Sbjct: 301 FEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVG 360

Query: 361 VFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQ 420
           VFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQ
Sbjct: 361 VFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQ 420

Query: 421 NDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFN 480
           NDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFN
Sbjct: 421 NDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFN 480

Query: 481 V 482
           V
Sbjct: 481 V 481

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BLAST of Carg18630 vs. NCBI nr
Match: XP_022938074.1 (cytokinin dehydrogenase 3-like [Cucurbita moschata])

HSP 1 Score: 970.3 bits (2507), Expect = 6.1e-279
Identity = 472/520 (90.77%), Postives = 479/520 (92.12%), Query Frame = 0


Query: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60
           MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP
Sbjct: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60

Query: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120
           AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET
Sbjct: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120

Query: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180
           PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG
Sbjct: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180

Query: 181 QSFRYGPQVSNVVEMDVVT---------------------------------------AP 240
           QSFRYGPQVSNVVEMDVVT                                       AP
Sbjct: 181 QSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVLGGLGQFGIIARARIALEPAP 240

Query: 241 RRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLEGLVLLHHDSPDTWRSSFFPPS 300
           +RVKW+RMLYTNFSAFAKDQERLISFNER+QANALDYLEGLVLLHHDSPDTWRSSFFPPS
Sbjct: 241 KRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFPPS 300

Query: 301 DHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVLFEGLDYESGFKFEKDVTYVKF 360
           DHPR+ISLANQNSLIYCLEVAKYYD HTQSTIDKD+EVLFEGLDYESGFKFEKDVTYVKF
Sbjct: 301 DHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVTYVKF 360

Query: 361 LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVGVFKDIVLRRNITKGPVLIYPM 420
           LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFD GVFKDIVLRRNITKGPVLIYPM
Sbjct: 361 LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVLIYPM 420

Query: 421 NHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPN 480
           NHSKWDSRNSTVIP+EEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPN
Sbjct: 421 NHSKWDSRNSTVIPNEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPN 480

Query: 481 YKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV 482
           +KTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV
Sbjct: 481 HKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV 520

[Close]

BLAST of Carg18630 vs. NCBI nr
Match: XP_022965677.1 (cytokinin dehydrogenase 3-like [Cucurbita maxima])

HSP 1 Score: 953.7 bits (2464), Expect = 5.9e-274
Identity = 466/520 (89.62%), Postives = 473/520 (90.96%), Query Frame = 0


Query: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60
           MTENLPVHFIVTFFINRLKSSI+KSKPWITLHPQPNNLV DPESLTLASSDYGNMVKEIP
Sbjct: 1   MTENLPVHFIVTFFINRLKSSISKSKPWITLHPQPNNLVDDPESLTLASSDYGNMVKEIP 60

Query: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120
           AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET
Sbjct: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120

Query: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180
           PGI ISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG
Sbjct: 121 PGITISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180

Query: 181 QSFRYGPQVSNVVEMDVVT---------------------------------------AP 240
           Q+FRYGPQVSNVVEMDVVT                                       AP
Sbjct: 181 QTFRYGPQVSNVVEMDVVTGQGNMLTCSAKKNSELFHAVLGGLGQFGIISRARIALEPAP 240

Query: 241 RRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLEGLVLLHHDSPDTWRSSFFPPS 300
            RVKWIRMLYTNF AFAKDQERLISFNER+QANALDYLEGLVLLHHDSPDTWRSSFFPPS
Sbjct: 241 NRVKWIRMLYTNFFAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFPPS 300

Query: 301 DHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVLFEGLDYESGFKFEKDVTYVKF 360
           DHPRIISLANQNS+IYCLEVAKYYDRHTQSTIDKD+EVLFEGLDYESGFKFEKDVTYVKF
Sbjct: 301 DHPRIISLANQNSVIYCLEVAKYYDRHTQSTIDKDLEVLFEGLDYESGFKFEKDVTYVKF 360

Query: 361 LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVGVFKDIVLRRNITKGPVLIYPM 420
           LNRVRSGELKLQSQ LWNVPHPWLNLFVPKSRI DFD GVFKDIVLRRNITKGPVLIYPM
Sbjct: 361 LNRVRSGELKLQSQALWNVPHPWLNLFVPKSRIFDFDAGVFKDIVLRRNITKGPVLIYPM 420

Query: 421 NHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPN 480
           N+SKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQN+EILEFCEKSGIEIKQYLPN
Sbjct: 421 NYSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQNEEILEFCEKSGIEIKQYLPN 480

Query: 481 YKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV 482
           YKTQRQWIDHFGSKWTNF DMKMKFDPKYILSPGQRIFNV
Sbjct: 481 YKTQRQWIDHFGSKWTNFGDMKMKFDPKYILSPGQRIFNV 520

[Close]

BLAST of Carg18630 vs. NCBI nr
Match: KAG6586478.1 (Cytokinin dehydrogenase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 911.8 bits (2355), Expect = 2.6e-261
Identity = 444/464 (95.69%), Postives = 444/464 (95.69%), Query Frame = 0


Query: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60
           MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP
Sbjct: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60

Query: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120
           AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET
Sbjct: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120

Query: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180
           PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG
Sbjct: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180

Query: 181 QSFRYGPQVSNVVEMDVVTAPRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLE 240
           QSFRYGPQVSNVVEMDVVT                    DQERLISFNERQQANALDYLE
Sbjct: 181 QSFRYGPQVSNVVEMDVVT--------------------DQERLISFNERQQANALDYLE 240

Query: 241 GLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVL 300
           GLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVL
Sbjct: 241 GLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVL 300

Query: 301 FEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVG 360
           FEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVG
Sbjct: 301 FEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVG 360

Query: 361 VFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQ 420
           VFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQ
Sbjct: 361 VFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQ 420

Query: 421 NDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMKM 465
           NDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMKM
Sbjct: 421 NDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMKM 444

[Close]

BLAST of Carg18630 vs. NCBI nr
Match: XP_023538489.1 (cytokinin dehydrogenase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 845.5 bits (2183), Expect = 2.3e-241
Identity = 422/520 (81.15%), Postives = 433/520 (83.27%), Query Frame = 0


Query: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60
           MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDP+SLTLASSDYGNMVKEIP
Sbjct: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPQSLTLASSDYGNMVKEIP 60

Query: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120
           AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNR+T
Sbjct: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRKT 120

Query: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180
           PGI ISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG
Sbjct: 121 PGITISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180

Query: 181 QSFRYGPQVSNVVEMDVVT---------------------------------------AP 240
           QSFRYGPQVSNVVEMDVVT                                       AP
Sbjct: 181 QSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNYELFHAVLGGLGQFGIIARARIALEPAP 240

Query: 241 RRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLEGLVLLHHDSPDTWRSSFFPPS 300
            RVKWIRMLYTNFSAF KDQERLISFNER+Q+NALDYLEGLVLLHH SPDTWRSSFFPPS
Sbjct: 241 NRVKWIRMLYTNFSAFVKDQERLISFNERKQSNALDYLEGLVLLHHGSPDTWRSSFFPPS 300

Query: 301 DHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVLFEGLDYESGFKFEKDVTYVKF 360
           DHPRIISLANQNS+IYC+EVAKYYDRHTQSTIDKD+EVLF+GLDYESGFKFEKDVTYVKF
Sbjct: 301 DHPRIISLANQNSIIYCIEVAKYYDRHTQSTIDKDLEVLFDGLDYESGFKFEKDVTYVKF 360

Query: 361 LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVGVFKDIVLRRNITKGPVLIYPM 420
           LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFD GVF+DIVLRRNITKGP+LIYPM
Sbjct: 361 LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAGVFRDIVLRRNITKGPILIYPM 420

Query: 421 NHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPN 480
           N+SKWDSRNSTVIPDEEV                                          
Sbjct: 421 NYSKWDSRNSTVIPDEEV-----------------------------------------K 479

Query: 481 YKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV 482
            KTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV
Sbjct: 481 MKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV 479

[Close]

BLAST of Carg18630 vs. ExPASy Swiss-Prot
Match: Q9LTS3 (Cytokinin dehydrogenase 3 OS=Arabidopsis thaliana OX=3702 GN=CKX3 PE=1 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 9.2e-145
Identity = 249/496 (50.20%), Postives = 340/496 (68.55%), Query Frame = 0


Query: 26  KPW-ITLHPQ-PNNLVHDPESLTLASSDYGNMVKEIPAAVLQPTSINDVVHLINYSYNNP 85
           +PW I  H +    L     S+  A++D+G++ K  P+AVL P+S+ D+  LI  S+++ 
Sbjct: 34  QPWNILSHNEFAGKLTSSSSSVESAATDFGHVTKIFPSAVLIPSSVEDITDLIKLSFDSQ 93

Query: 86  IPFPVAARGQGHSVRGQAMAKNGVVIDM-SYLRRNRETPGIIISRLRSTGEIYADVGGEQ 145
           + FP+AARG GHS RGQA AK+GVV++M S + R+R   GI +SR      +Y DV    
Sbjct: 94  LSFPLAARGHGHSHRGQASAKDGVVVNMRSMVNRDR---GIKVSRTC----LYVDVDAAW 153

Query: 146 LWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVT--- 205
           LWI+VLN  L  G+TPVSWTDYLY+TVGGTLSNGGISGQ+FRYGPQ++NV+EMDV+T   
Sbjct: 154 LWIEVLNKTLELGLTPVSWTDYLYLTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKG 213

Query: 206 ------------------------------------APRRVKWIRMLYTNFSAFAKDQER 265
                                               AP+R KW+R LY +FS F +DQER
Sbjct: 214 EIATCSKDMNSDLFFAVLGGLGQFGIITRARIKLEVAPKRAKWLRFLYIDFSEFTRDQER 273

Query: 266 LISFNERQQANALDYLEGLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAK 325
           +IS     + + +D+LEG +++ H  PD WRS+++PPSDH RI S+  ++ +IYCLEV K
Sbjct: 274 VIS-----KTDGVDFLEGSIMVDHGPPDNWRSTYYPPSDHLRIASMVKRHRVIYCLEVVK 333

Query: 326 YYDRHTQSTIDKDIEVLFEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHP 385
           YYD  +Q T+++++E L + L++  GF +EKDVTY+ FLNRVR+GEL L+S+G W+VPHP
Sbjct: 334 YYDETSQYTVNEEMEELSDSLNHVRGFMYEKDVTYMDFLNRVRTGELNLKSKGQWDVPHP 393

Query: 386 WLNLFVPKSRILDFDVGVFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPDEEVFYTI 445
           WLNLFVPK++I  FD GVFK I+LR NIT GPVL+YPMN +KW+ R S  IP+E+VFY +
Sbjct: 394 WLNLFVPKTQISKFDDGVFKGIILRNNITSGPVLVYPMNRNKWNDRMSAAIPEEDVFYAV 453

Query: 446 GFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMK 480
           GFL S+GFD+W++FD++N EIL+FCE + + + QYLP + +Q  W+ HFG +W  F + K
Sbjct: 454 GFLRSAGFDNWEAFDQENMEILKFCEDANMGVIQYLPYHSSQEGWVRHFGPRWNIFVERK 513

[Close]

BLAST of Carg18630 vs. ExPASy Swiss-Prot
Match: Q9FUJ2 (Cytokinin dehydrogenase 4 OS=Arabidopsis thaliana OX=3702 GN=CKX4 PE=1 SV=2)

HSP 1 Score: 441.4 bits (1134), Expect = 1.3e-122
Identity = 238/523 (45.51%), Postives = 315/523 (60.23%), Query Frame = 0


Query: 10  IVTFFINRLKSSINKSKPWITLHPQPNNL--VHDPESLTLASSDYGNMVKEIPAAVLQPT 69
           ++T FI+   + I   +      P   NL  + DP S++ AS D+GN+  E P AVL P+
Sbjct: 12  LITLFISLTPTLIKSDEGIDVFLPISLNLTVLTDPFSISAASHDFGNITDENPGAVLCPS 71

Query: 70  SINDVVHLI-----NYSYNN-----PIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRN 129
           S  +V  L+      +SYN         F VAARGQGHS+RGQA A  GVV++M+ L   
Sbjct: 72  STTEVARLLRFANGGFSYNKGSTSPASTFKVAARGQGHSLRGQASAPGGVVVNMTCLAMA 131

Query: 130 RETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGG 189
            +   ++I    S    YADV    +W+DVL AA+  GV+PV+WTDYLY++VGGTLSN G
Sbjct: 132 AKPAAVVI----SADGTYADVAAGTMWVDVLKAAVDRGVSPVTWTDYLYLSVGGTLSNAG 191

Query: 190 ISGQSFRYGPQVSNVVEMDVVT-------------------------------------- 249
           I GQ+FR+GPQ+SNV E+DV+T                                      
Sbjct: 192 IGGQTFRHGPQISNVHELDVITGKGEMMTCSPKLNPELFYGVLGGLGQFGIITRARIALD 251

Query: 250 -APRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLEGLVLLHHDSPDTWRSSFF 309
            AP RVKW R+LY++FSAF +DQERLIS         +D+LEG +++ +   DT   SFF
Sbjct: 252 HAPTRVKWSRILYSDFSAFKRDQERLISMTNDL---GVDFLEGQLMMSNGFVDT---SFF 311

Query: 310 PPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVLFEGLDYESGFKFEKDVTY 369
           P SD  R+ SL N + +IY LEVAKYYDR T   ID+ I+ L   L +  GF F +DV Y
Sbjct: 312 PLSDQTRVASLVNDHRIIYVLEVAKYYDRTTLPIIDQVIDTLSRTLGFAPGFMFVQDVPY 371

Query: 370 VKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVGVFKDIVLRRNITKGPVLI 429
             FLNRVR+ E KL+S GLW VPHPWLN+FVP SRI DF  GV   ++L +  T G  L 
Sbjct: 372 FDFLNRVRNEEDKLRSLGLWEVPHPWLNIFVPGSRIQDFHDGVINGLLLNQTSTSGVTLF 431

Query: 430 YPMNHSKWDSRNSTVIPDEEVFYTIGFLHSS-GFDDWKSFDRQNDEILEFCEKSGIEIKQ 481
           YP N +KW++R ST+ PDE+VFY IG L S+ G  +W+  +  ND++++FCE SGI+IK+
Sbjct: 432 YPTNRNKWNNRMSTMTPDEDVFYVIGLLQSAGGSQNWQELENLNDKVIQFCENSGIKIKE 491

[Close]

BLAST of Carg18630 vs. ExPASy Swiss-Prot
Match: Q67YU0 (Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 7.1e-121
Identity = 223/484 (46.07%), Postives = 299/484 (61.78%), Query Frame = 0


Query: 42  PESLTLASSDYGNMVK-EIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQ 101
           P  L   SSD+G +   E P AVL P+S  DV  L+  +Y +   FPV+ARG GHS+ GQ
Sbjct: 48  PSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAYGSATAFPVSARGHGHSINGQ 107

Query: 102 AMA-KNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPV 161
           A A +NGVV++M++       P      L    E+Y DV G +LW+DVL   L +G+ P 
Sbjct: 108 AAAGRNGVVVEMNHGVTGTPKP------LVRPDEMYVDVWGGELWVDVLKKTLEHGLAPK 167

Query: 162 SWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVT-------------------- 221
           SWTDYLY+TVGGTLSN GISGQ+F +GPQ+SNV+E+DVVT                    
Sbjct: 168 SWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEENTRLFHGV 227

Query: 222 -------------------APRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLE 281
                              AP+RV+WIR+LY++F  F +DQE LIS + + +    DY+E
Sbjct: 228 LGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLK---FDYVE 287

Query: 282 GLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVL 341
           G V++     + WRSSFF P +  +I S+++  S++YCLE+ K Y       +D+++E+L
Sbjct: 288 GFVIVDEGLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQEVEIL 347

Query: 342 FEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVG 401
            + L++     F  D+ YV FL+RV   ELKL+S+ LW VPHPWLNLFVPKSRI DFD G
Sbjct: 348 MKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISDFDKG 407

Query: 402 VFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKS---- 461
           VFK I+   N T GP+LIYPMN  KWD R+S V PDEEVFY +  L S+  D  ++    
Sbjct: 408 VFKGIL--GNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQKLE 467

Query: 462 -FDRQNDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPG 480
               QN  ILEFCE++ I +KQYLP++ TQ +W+ HFG KW  FR +K +FDP++IL+ G
Sbjct: 468 YLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHILATG 520

[Close]

BLAST of Carg18630 vs. ExPASy Swiss-Prot
Match: Q5ZAY9 (Cytokinin dehydrogenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX5 PE=2 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 4.3e-118
Identity = 222/486 (45.68%), Postives = 291/486 (59.88%), Query Frame = 0


Query: 41  DPESLTLASSDYGNMVKEIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQ 100
           +P  +  AS D+G +    P AV  P    DV  L+  +Y +     V+ARG GHS+ GQ
Sbjct: 44  EPSDVMEASLDFGRLTSAEPLAVFHPRGAGDVAALVKAAYGSASGIRVSARGHGHSISGQ 103

Query: 101 AMAKNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAY-GVTPV 160
           A A  GVV+DMS+  R       +     + G  Y DV G +LWIDVLN  LA+ G+ P 
Sbjct: 104 AQAAGGVVVDMSHGWRAEAAERTLPVYSPALGGHYIDVWGGELWIDVLNWTLAHGGLAPR 163

Query: 161 SWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVT-------------------- 220
           SWTDYLY++VGGTLSN GISGQ+F +GPQ+SNV E+DVVT                    
Sbjct: 164 SWTDYLYLSVGGTLSNAGISGQAFHHGPQISNVYELDVVTGKGEVVTCSESNNPDLFFGA 223

Query: 221 -------------------APRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLE 280
                              AP RV+WIR LY+NF+ F  DQERLIS   +      DY+E
Sbjct: 224 LGGLGQLGIITRARIALEPAPHRVRWIRALYSNFTEFTADQERLISL--QHGGRRFDYVE 283

Query: 281 GLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVL 340
           G V+      + WRSSFF P +  ++ SL + + ++YCLEV K YD  T  T+D+D+E L
Sbjct: 284 GFVVAAEGLINNWRSSFFSPQNPVKLSSLKHHSGVLYCLEVTKNYDDSTAVTVDQDVEAL 343

Query: 341 FEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVG 400
              L++  G  F  D+ YV FL+RV   ELKL+ +G+W VPHPWLNLFVP SRI DFD G
Sbjct: 344 LGELNFIPGTVFTTDLPYVDFLDRVHKAELKLRGKGMWEVPHPWLNLFVPASRIADFDRG 403

Query: 401 VFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPDEEVFYTIGFLHSS--GFDD----W 460
           VF+  VL      GP+LIYPMN  KWD R+S V P+E+VFY + FL S+  G  D     
Sbjct: 404 VFRG-VLGSRTAGGPILIYPMNRHKWDPRSSVVTPEEDVFYLVAFLRSAVPGSTDPAQSL 463

Query: 461 KSFDRQNDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSP 481
           ++ +RQN EILEFC+++GI  KQYLPN+K QR+W  HFG++W  F  +K +FDP+ +L+ 
Sbjct: 464 EALERQNREILEFCDEAGIGAKQYLPNHKAQREWEAHFGARWARFARLKAEFDPRAMLAT 523

[Close]

BLAST of Carg18630 vs. ExPASy Swiss-Prot
Match: Q9FUJ3 (Cytokinin dehydrogenase 2 OS=Arabidopsis thaliana OX=3702 GN=CKX2 PE=1 SV=2)

HSP 1 Score: 421.4 bits (1082), Expect = 1.4e-116
Identity = 225/482 (46.68%), Postives = 288/482 (59.75%), Query Frame = 0


Query: 41  DPESLTLASSDYGNMVKEIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQ 100
           DP  ++ AS D+GN+    P  V+ P+S  D+  L+ Y+ N    F VAARGQGHS+ GQ
Sbjct: 38  DPSIISAASHDFGNITTVTPGGVICPSSTADISRLLQYAANGKSTFQVAARGQGHSLNGQ 97

Query: 101 AMAKNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVS 160
           A    GV+++M+ +        +++S+ +     YADV    LW+DVL      GV+PVS
Sbjct: 98  ASVSGGVIVNMTCI------TDVVVSKDKK----YADVAAGTLWVDVLKKTAEKGVSPVS 157

Query: 161 WTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVT--------------------- 220
           WTDYL+ITVGGTLSNGGI GQ FR GP VSNV+E+DV+T                     
Sbjct: 158 WTDYLHITVGGTLSNGGIGGQVFRNGPLVSNVLELDVITGKGEMLTCSRQLNPELFYGVL 217

Query: 221 ------------------APRRVKWIRMLYTNFSAFAKDQERLISFNERQQAN--ALDYL 280
                             AP+R KW RMLY++F+ F KDQERLIS      AN   +DYL
Sbjct: 218 GGLGQFGIITRARIVLDHAPKRAKWFRMLYSDFTTFTKDQERLIS-----MANDIGVDYL 277

Query: 281 EGLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEV 340
           EG + L +   DT   SFFPPSD  ++  L  Q+ +IY LEVAKYYD      I K I+ 
Sbjct: 278 EGQIFLSNGVVDT---SFFPPSDQSKVADLVKQHGIIYVLEVAKYYDDPNLPIISKVIDT 337

Query: 341 LFEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDV 400
           L + L Y  GF    DV Y  FLNRV   E KL+S GLW +PHPWLNL+VPKSRILDF  
Sbjct: 338 LTKTLSYLPGFISMHDVAYFDFLNRVHVEENKLRSLGLWELPHPWLNLYVPKSRILDFHN 397

Query: 401 GVFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIP--DEEVFYTIGFLHSSGFDDWKSF 460
           GV KDI+L++    G  L+YP N +KWD+R S +IP  DE+V Y IG L S+   D    
Sbjct: 398 GVVKDILLKQKSASGLALLYPTNRNKWDNRMSAMIPEIDEDVIYIIGLLQSATPKDLPEV 457

Query: 461 DRQNDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQR 480
           +  N++I+ FC+ SGI+IKQYL +Y ++  WI+HFGSKW +F   K  FDPK +LSPGQ 
Sbjct: 458 ESVNEKIIRFCKDSGIKIKQYLMHYTSKEDWIEHFGSKWDDFSKRKDLFDPKKLLSPGQD 501

[Close]

BLAST of Carg18630 vs. ExPASy TrEMBL
Match: A0A6J1FD08 (Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111444271 PE=3 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 2.9e-279
Identity = 472/520 (90.77%), Postives = 479/520 (92.12%), Query Frame = 0


Query: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60
           MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP
Sbjct: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60

Query: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120
           AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET
Sbjct: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120

Query: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180
           PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG
Sbjct: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180

Query: 181 QSFRYGPQVSNVVEMDVVT---------------------------------------AP 240
           QSFRYGPQVSNVVEMDVVT                                       AP
Sbjct: 181 QSFRYGPQVSNVVEMDVVTGKGNMVTCSAKKNSELFHAVLGGLGQFGIIARARIALEPAP 240

Query: 241 RRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLEGLVLLHHDSPDTWRSSFFPPS 300
           +RVKW+RMLYTNFSAFAKDQERLISFNER+QANALDYLEGLVLLHHDSPDTWRSSFFPPS
Sbjct: 241 KRVKWVRMLYTNFSAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFPPS 300

Query: 301 DHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVLFEGLDYESGFKFEKDVTYVKF 360
           DHPR+ISLANQNSLIYCLEVAKYYD HTQSTIDKD+EVLFEGLDYESGFKFEKDVTYVKF
Sbjct: 301 DHPRVISLANQNSLIYCLEVAKYYDHHTQSTIDKDLEVLFEGLDYESGFKFEKDVTYVKF 360

Query: 361 LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVGVFKDIVLRRNITKGPVLIYPM 420
           LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFD GVFKDIVLRRNITKGPVLIYPM
Sbjct: 361 LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDAGVFKDIVLRRNITKGPVLIYPM 420

Query: 421 NHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPN 480
           NHSKWDSRNSTVIP+EEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPN
Sbjct: 421 NHSKWDSRNSTVIPNEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPN 480

Query: 481 YKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV 482
           +KTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV
Sbjct: 481 HKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV 520

[Close]

BLAST of Carg18630 vs. ExPASy TrEMBL
Match: A0A6J1HKZ4 (Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111465505 PE=3 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 2.8e-274
Identity = 466/520 (89.62%), Postives = 473/520 (90.96%), Query Frame = 0


Query: 1   MTENLPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKEIP 60
           MTENLPVHFIVTFFINRLKSSI+KSKPWITLHPQPNNLV DPESLTLASSDYGNMVKEIP
Sbjct: 1   MTENLPVHFIVTFFINRLKSSISKSKPWITLHPQPNNLVDDPESLTLASSDYGNMVKEIP 60

Query: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120
           AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET
Sbjct: 61  AAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNRET 120

Query: 121 PGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180
           PGI ISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG
Sbjct: 121 PGITISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISG 180

Query: 181 QSFRYGPQVSNVVEMDVVT---------------------------------------AP 240
           Q+FRYGPQVSNVVEMDVVT                                       AP
Sbjct: 181 QTFRYGPQVSNVVEMDVVTGQGNMLTCSAKKNSELFHAVLGGLGQFGIISRARIALEPAP 240

Query: 241 RRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLEGLVLLHHDSPDTWRSSFFPPS 300
            RVKWIRMLYTNF AFAKDQERLISFNER+QANALDYLEGLVLLHHDSPDTWRSSFFPPS
Sbjct: 241 NRVKWIRMLYTNFFAFAKDQERLISFNERKQANALDYLEGLVLLHHDSPDTWRSSFFPPS 300

Query: 301 DHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVLFEGLDYESGFKFEKDVTYVKF 360
           DHPRIISLANQNS+IYCLEVAKYYDRHTQSTIDKD+EVLFEGLDYESGFKFEKDVTYVKF
Sbjct: 301 DHPRIISLANQNSVIYCLEVAKYYDRHTQSTIDKDLEVLFEGLDYESGFKFEKDVTYVKF 360

Query: 361 LNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVGVFKDIVLRRNITKGPVLIYPM 420
           LNRVRSGELKLQSQ LWNVPHPWLNLFVPKSRI DFD GVFKDIVLRRNITKGPVLIYPM
Sbjct: 361 LNRVRSGELKLQSQALWNVPHPWLNLFVPKSRIFDFDAGVFKDIVLRRNITKGPVLIYPM 420

Query: 421 NHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPN 480
           N+SKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQN+EILEFCEKSGIEIKQYLPN
Sbjct: 421 NYSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQNEEILEFCEKSGIEIKQYLPN 480

Query: 481 YKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV 482
           YKTQRQWIDHFGSKWTNF DMKMKFDPKYILSPGQRIFNV
Sbjct: 481 YKTQRQWIDHFGSKWTNFGDMKMKFDPKYILSPGQRIFNV 520

[Close]

BLAST of Carg18630 vs. ExPASy TrEMBL
Match: A0A1S3C9Z6 (Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498521 PE=3 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 4.9e-234
Identity = 399/523 (76.29%), Postives = 440/523 (84.13%), Query Frame = 0


Query: 1   MTENLPV--HFIVTFFINRLKSSINKSKPW-ITLHPQPNNLVHDPESLTLASSDYGNMVK 60
           M ENLPV  +FI+TFFI+RLKSSINKSK W  TL+ +PNNL++DPE+LTLASSDYGNMVK
Sbjct: 1   MNENLPVPAYFIITFFISRLKSSINKSKAWTTTLNQRPNNLLNDPETLTLASSDYGNMVK 60

Query: 61  EIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRN 120
           E PAAVLQP+SIND+V LI+Y+YNNPIPF +AARGQGHSVRGQAMAKNGVVIDMS LRRN
Sbjct: 61  ETPAAVLQPSSINDIVQLISYAYNNPIPFQIAARGQGHSVRGQAMAKNGVVIDMSALRRN 120

Query: 121 RETPGIIIS-RLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNG 180
           R+TPGI++S R  +TGE Y DVGGEQLWIDVLN  L YG+TPVSWTDYLYITVGGTLSN 
Sbjct: 121 RKTPGIVVSCRRWTTGEFYVDVGGEQLWIDVLNETLGYGMTPVSWTDYLYITVGGTLSNA 180

Query: 181 GISGQSFRYGPQVSNVVEMDVVT------------------------------------- 240
           GISGQSFRYGPQVSNVVEMDVVT                                     
Sbjct: 181 GISGQSFRYGPQVSNVVEMDVVTGKGNMMTCSPKKNCELFHAVLGGLGQFGIIARARIAL 240

Query: 241 --APRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLEGLVLLHHDSPDTWRSSF 300
             AP RVKW+RMLYTNF+AF KDQE LIS N R+Q NALDYLEGLVLLHH+ PD WRSSF
Sbjct: 241 EPAPIRVKWVRMLYTNFAAFTKDQEHLISLNGRKQMNALDYLEGLVLLHHNKPDNWRSSF 300

Query: 301 FPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVLFEGLDYESGFKFEKDVT 360
           FPPSDH RIISLANQNS+IYCLEV KYYD HTQST+DKD+EVL +GLDYESGFKFEKDVT
Sbjct: 301 FPPSDHSRIISLANQNSIIYCLEVVKYYDHHTQSTVDKDLEVLLQGLDYESGFKFEKDVT 360

Query: 361 YVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVGVFKDIVLRRNITKGPVL 420
           YV+FLNRVR+GELKLQS+GLW+VPHPWLNLFVP+SRI+DF+ GVFKDIVLRRNITKGP+L
Sbjct: 361 YVEFLNRVRTGELKLQSKGLWDVPHPWLNLFVPRSRIMDFNSGVFKDIVLRRNITKGPIL 420

Query: 421 IYPMNHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQ 480
           IYPMN SKWD RNSTVIPDEEVFYTIGFL+SSGFDDWK F+ QN+EILE+C KSGIEIKQ
Sbjct: 421 IYPMNRSKWDERNSTVIPDEEVFYTIGFLNSSGFDDWKKFEEQNEEILEYCGKSGIEIKQ 480

[Close]

BLAST of Carg18630 vs. ExPASy TrEMBL
Match: A0A6J1G4H4 (Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111450699 PE=3 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 2.1e-232
Identity = 396/522 (75.86%), Postives = 433/522 (82.95%), Query Frame = 0


Query: 1   MTEN--LPVHFIVTFFINRLKSSINKSKPWITLHPQPNNLVHDPESLTLASSDYGNMVKE 60
           MTEN  LP +FI+TFF++ LKS INKSKPW  LH QPN L HDPE+L LA+SDYGNMVKE
Sbjct: 1   MTENHPLPAYFILTFFLSCLKSGINKSKPWTGLHQQPNKLRHDPEALNLAASDYGNMVKE 60

Query: 61  IPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRNR 120
            PAAVLQP+SINDVVHLI+Y+YNNPIPFP+AARGQGHSVRGQAMAKNGVVIDMS LRRNR
Sbjct: 61  APAAVLQPSSINDVVHLISYAYNNPIPFPIAARGQGHSVRGQAMAKNGVVIDMSALRRNR 120

Query: 121 ETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGI 180
           +T GI +   R TGE+YADVGGEQLWIDVLNA LAYGVTPVSWTDYLYITVGGTLSNGGI
Sbjct: 121 KTAGIAVFARRWTGELYADVGGEQLWIDVLNATLAYGVTPVSWTDYLYITVGGTLSNGGI 180

Query: 181 SGQSFRYGPQVSNVVEMDVVT--------------------------------------- 240
           SGQSFRYGPQVSNVVEMDVVT                                       
Sbjct: 181 SGQSFRYGPQVSNVVEMDVVTGKGNMVTCSAKRNSELFHAVLGGLGQFGIIAQARIALEP 240

Query: 241 APRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLEGLVLLHHDSPDTWRSSFFP 300
           AP RVKW+RMLY+NFSAF KDQERLIS N R+QA+ALDYLEGLVLLHH SPD+WRSSFFP
Sbjct: 241 APNRVKWVRMLYSNFSAFTKDQERLISLNGRKQASALDYLEGLVLLHHSSPDSWRSSFFP 300

Query: 301 PSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVLFEGLDYESGFKFEKDVTYV 360
           PSDHPRIIS  NQ S++YCLEVAKYYD HT ST+DK++EVL   LDYESGFKFEKDVTYV
Sbjct: 301 PSDHPRIISFVNQYSVVYCLEVAKYYDHHTLSTVDKELEVLLAELDYESGFKFEKDVTYV 360

Query: 361 KFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVGVFKDIVLRRNITKGPVLIY 420
           +FLNRVR+GELKLQS+GLW+V HPWLNLFVPKSRILDFD GVFKDIVLRRNITKGPVLIY
Sbjct: 361 EFLNRVRTGELKLQSKGLWDVSHPWLNLFVPKSRILDFDSGVFKDIVLRRNITKGPVLIY 420

Query: 421 PMNHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYL 480
           PMN SKWD RNSTVIP+EEVFYTIGFLHSSGFD+ K FD QN+EI+EFC KSGI+IKQYL
Sbjct: 421 PMNRSKWDDRNSTVIPEEEVFYTIGFLHSSGFDERKLFDEQNEEIIEFCTKSGIQIKQYL 480

Query: 481 PNYKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQRIFNV 482
           P+Y TQ QWI HFGSKW  FR  K+K+DPK ILSPG +IFN+
Sbjct: 481 PHYXTQTQWIHHFGSKWPTFRQRKIKYDPKLILSPGHKIFNL 522

[Close]

BLAST of Carg18630 vs. ExPASy TrEMBL
Match: A0A6J1DPL8 (Cytokinin dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111021923 PE=3 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 4.9e-226
Identity = 390/523 (74.57%), Postives = 427/523 (81.64%), Query Frame = 0


Query: 1   MTENLPV--HFIVTFFINRLKSSINKSKPWITLHPQPN--NLVHDPESLTLASSDYGNMV 60
           MTENLPV  +FIVTFFI+RLKSS+ KSKPW + H QPN   L  DPE+L  AS+DYGNMV
Sbjct: 1   MTENLPVPAYFIVTFFISRLKSSMGKSKPWTSFHLQPNKLRLRDDPEALNSASTDYGNMV 60

Query: 61  KEIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRR 120
           KE PAAVL P+SINDVV LI+Y+YNNPIPF +AARGQGHSVRGQAMA++GVVIDMS LRR
Sbjct: 61  KETPAAVLNPSSINDVVQLISYAYNNPIPFQIAARGQGHSVRGQAMARDGVVIDMSSLRR 120

Query: 121 NRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNG 180
           NR TPGI +    STGE YADVGGEQ WIDVLNA L YG+TPVSWTDYLYITVGGTLSN 
Sbjct: 121 NRATPGITV----STGEFYADVGGEQRWIDVLNATLEYGLTPVSWTDYLYITVGGTLSNA 180

Query: 181 GISGQSFRYGPQVSNVVEMDVVT------------------------------------- 240
           GISGQ+FRYGPQVSNVVEMDVVT                                     
Sbjct: 181 GISGQTFRYGPQVSNVVEMDVVTGKGNMVTCSAKRDSELFHAVLGGLGQFGIIARARIVL 240

Query: 241 --APRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLEGLVLLHHDSPDTWRSSF 300
             AP RVKW+RMLY+NFS FAKDQE LIS + R+Q NALDYLEGLVLLHH SPD WRSSF
Sbjct: 241 ERAPTRVKWVRMLYSNFSEFAKDQENLISLHGRKQENALDYLEGLVLLHHGSPDNWRSSF 300

Query: 301 FPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVLFEGLDYESGFKFEKDVT 360
           FPPSDHPRII+LANQNSLIYCLEV KYYD  TQS +DK+++ L EGL YE GFKFEKDV+
Sbjct: 301 FPPSDHPRIIALANQNSLIYCLEVVKYYDDSTQSIVDKEVDALLEGLGYERGFKFEKDVS 360

Query: 361 YVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVGVFKDIVLRRNITKGPVL 420
           YV+FLNRVRSGELKLQSQGLW+VPHPWLNLF+PKSRI DFD GVFKDIVLR NITKGPVL
Sbjct: 361 YVEFLNRVRSGELKLQSQGLWDVPHPWLNLFIPKSRISDFDSGVFKDIVLRHNITKGPVL 420

Query: 421 IYPMNHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQ 480
           IYPMN SKWDSRNSTVIPDEEVFYTIGFLHSSGF DWKSFD QN+EILEFC KSGIEIKQ
Sbjct: 421 IYPMNRSKWDSRNSTVIPDEEVFYTIGFLHSSGFGDWKSFDEQNEEILEFCAKSGIEIKQ 480

[Close]

BLAST of Carg18630 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 515.0 bits (1325), Expect = 6.5e-146
Identity = 249/496 (50.20%), Postives = 340/496 (68.55%), Query Frame = 0


Query: 26  KPW-ITLHPQ-PNNLVHDPESLTLASSDYGNMVKEIPAAVLQPTSINDVVHLINYSYNNP 85
           +PW I  H +    L     S+  A++D+G++ K  P+AVL P+S+ D+  LI  S+++ 
Sbjct: 34  QPWNILSHNEFAGKLTSSSSSVESAATDFGHVTKIFPSAVLIPSSVEDITDLIKLSFDSQ 93

Query: 86  IPFPVAARGQGHSVRGQAMAKNGVVIDM-SYLRRNRETPGIIISRLRSTGEIYADVGGEQ 145
           + FP+AARG GHS RGQA AK+GVV++M S + R+R   GI +SR      +Y DV    
Sbjct: 94  LSFPLAARGHGHSHRGQASAKDGVVVNMRSMVNRDR---GIKVSRTC----LYVDVDAAW 153

Query: 146 LWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVT--- 205
           LWI+VLN  L  G+TPVSWTDYLY+TVGGTLSNGGISGQ+FRYGPQ++NV+EMDV+T   
Sbjct: 154 LWIEVLNKTLELGLTPVSWTDYLYLTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKG 213

Query: 206 ------------------------------------APRRVKWIRMLYTNFSAFAKDQER 265
                                               AP+R KW+R LY +FS F +DQER
Sbjct: 214 EIATCSKDMNSDLFFAVLGGLGQFGIITRARIKLEVAPKRAKWLRFLYIDFSEFTRDQER 273

Query: 266 LISFNERQQANALDYLEGLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAK 325
           +IS     + + +D+LEG +++ H  PD WRS+++PPSDH RI S+  ++ +IYCLEV K
Sbjct: 274 VIS-----KTDGVDFLEGSIMVDHGPPDNWRSTYYPPSDHLRIASMVKRHRVIYCLEVVK 333

Query: 326 YYDRHTQSTIDKDIEVLFEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHP 385
           YYD  +Q T+++++E L + L++  GF +EKDVTY+ FLNRVR+GEL L+S+G W+VPHP
Sbjct: 334 YYDETSQYTVNEEMEELSDSLNHVRGFMYEKDVTYMDFLNRVRTGELNLKSKGQWDVPHP 393

Query: 386 WLNLFVPKSRILDFDVGVFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPDEEVFYTI 445
           WLNLFVPK++I  FD GVFK I+LR NIT GPVL+YPMN +KW+ R S  IP+E+VFY +
Sbjct: 394 WLNLFVPKTQISKFDDGVFKGIILRNNITSGPVLVYPMNRNKWNDRMSAAIPEEDVFYAV 453

Query: 446 GFLHSSGFDDWKSFDRQNDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMK 480
           GFL S+GFD+W++FD++N EIL+FCE + + + QYLP + +Q  W+ HFG +W  F + K
Sbjct: 454 GFLRSAGFDNWEAFDQENMEILKFCEDANMGVIQYLPYHSSQEGWVRHFGPRWNIFVERK 513

[Close]

BLAST of Carg18630 vs. TAIR 10
Match: AT4G29740.2 (cytokinin oxidase 4 )

HSP 1 Score: 441.4 bits (1134), Expect = 9.1e-124
Identity = 238/523 (45.51%), Postives = 315/523 (60.23%), Query Frame = 0


Query: 10  IVTFFINRLKSSINKSKPWITLHPQPNNL--VHDPESLTLASSDYGNMVKEIPAAVLQPT 69
           ++T FI+   + I   +      P   NL  + DP S++ AS D+GN+  E P AVL P+
Sbjct: 12  LITLFISLTPTLIKSDEGIDVFLPISLNLTVLTDPFSISAASHDFGNITDENPGAVLCPS 71

Query: 70  SINDVVHLI-----NYSYNN-----PIPFPVAARGQGHSVRGQAMAKNGVVIDMSYLRRN 129
           S  +V  L+      +SYN         F VAARGQGHS+RGQA A  GVV++M+ L   
Sbjct: 72  STTEVARLLRFANGGFSYNKGSTSPASTFKVAARGQGHSLRGQASAPGGVVVNMTCLAMA 131

Query: 130 RETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVSWTDYLYITVGGTLSNGG 189
            +   ++I    S    YADV    +W+DVL AA+  GV+PV+WTDYLY++VGGTLSN G
Sbjct: 132 AKPAAVVI----SADGTYADVAAGTMWVDVLKAAVDRGVSPVTWTDYLYLSVGGTLSNAG 191

Query: 190 ISGQSFRYGPQVSNVVEMDVVT-------------------------------------- 249
           I GQ+FR+GPQ+SNV E+DV+T                                      
Sbjct: 192 IGGQTFRHGPQISNVHELDVITGKGEMMTCSPKLNPELFYGVLGGLGQFGIITRARIALD 251

Query: 250 -APRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLEGLVLLHHDSPDTWRSSFF 309
            AP RVKW R+LY++FSAF +DQERLIS         +D+LEG +++ +   DT   SFF
Sbjct: 252 HAPTRVKWSRILYSDFSAFKRDQERLISMTNDL---GVDFLEGQLMMSNGFVDT---SFF 311

Query: 310 PPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVLFEGLDYESGFKFEKDVTY 369
           P SD  R+ SL N + +IY LEVAKYYDR T   ID+ I+ L   L +  GF F +DV Y
Sbjct: 312 PLSDQTRVASLVNDHRIIYVLEVAKYYDRTTLPIIDQVIDTLSRTLGFAPGFMFVQDVPY 371

Query: 370 VKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVGVFKDIVLRRNITKGPVLI 429
             FLNRVR+ E KL+S GLW VPHPWLN+FVP SRI DF  GV   ++L +  T G  L 
Sbjct: 372 FDFLNRVRNEEDKLRSLGLWEVPHPWLNIFVPGSRIQDFHDGVINGLLLNQTSTSGVTLF 431

Query: 430 YPMNHSKWDSRNSTVIPDEEVFYTIGFLHSS-GFDDWKSFDRQNDEILEFCEKSGIEIKQ 481
           YP N +KW++R ST+ PDE+VFY IG L S+ G  +W+  +  ND++++FCE SGI+IK+
Sbjct: 432 YPTNRNKWNNRMSTMTPDEDVFYVIGLLQSAGGSQNWQELENLNDKVIQFCENSGIKIKE 491

[Close]

BLAST of Carg18630 vs. TAIR 10
Match: AT1G75450.1 (cytokinin oxidase 5 )

HSP 1 Score: 435.6 bits (1119), Expect = 5.0e-122
Identity = 223/484 (46.07%), Postives = 299/484 (61.78%), Query Frame = 0


Query: 42  PESLTLASSDYGNMVK-EIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQ 101
           P  L   SSD+G +   E P AVL P+S  DV  L+  +Y +   FPV+ARG GHS+ GQ
Sbjct: 48  PSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAYGSATAFPVSARGHGHSINGQ 107

Query: 102 AMA-KNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPV 161
           A A +NGVV++M++       P      L    E+Y DV G +LW+DVL   L +G+ P 
Sbjct: 108 AAAGRNGVVVEMNHGVTGTPKP------LVRPDEMYVDVWGGELWVDVLKKTLEHGLAPK 167

Query: 162 SWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVT-------------------- 221
           SWTDYLY+TVGGTLSN GISGQ+F +GPQ+SNV+E+DVVT                    
Sbjct: 168 SWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEENTRLFHGV 227

Query: 222 -------------------APRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYLE 281
                              AP+RV+WIR+LY++F  F +DQE LIS + + +    DY+E
Sbjct: 228 LGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLK---FDYVE 287

Query: 282 GLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEVL 341
           G V++     + WRSSFF P +  +I S+++  S++YCLE+ K Y       +D+++E+L
Sbjct: 288 GFVIVDEGLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQEVEIL 347

Query: 342 FEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDVG 401
            + L++     F  D+ YV FL+RV   ELKL+S+ LW VPHPWLNLFVPKSRI DFD G
Sbjct: 348 MKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISDFDKG 407

Query: 402 VFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKS---- 461
           VFK I+   N T GP+LIYPMN  KWD R+S V PDEEVFY +  L S+  D  ++    
Sbjct: 408 VFKGIL--GNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQKLE 467

Query: 462 -FDRQNDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPG 480
               QN  ILEFCE++ I +KQYLP++ TQ +W+ HFG KW  FR +K +FDP++IL+ G
Sbjct: 468 YLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHILATG 520

[Close]

BLAST of Carg18630 vs. TAIR 10
Match: AT1G75450.2 (cytokinin oxidase 5 )

HSP 1 Score: 435.3 bits (1118), Expect = 6.6e-122
Identity = 223/485 (45.98%), Postives = 299/485 (61.65%), Query Frame = 0


Query: 42  PESLTLASSDYGNMVK-EIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQ 101
           P  L   SSD+G +   E P AVL P+S  DV  L+  +Y +   FPV+ARG GHS+ GQ
Sbjct: 44  PSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAYGSATAFPVSARGHGHSINGQ 103

Query: 102 AMA-KNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPV 161
           A A +NGVV++M++       P      L    E+Y DV G +LW+DVL   L +G+ P 
Sbjct: 104 AAAGRNGVVVEMNHGVTGTPKP------LVRPDEMYVDVWGGELWVDVLKKTLEHGLAPK 163

Query: 162 SWTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVT-------------------- 221
           SWTDYLY+TVGGTLSN GISGQ+F +GPQ+SNV+E+DVVT                    
Sbjct: 164 SWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTVGKGEVMRCSEEENTRLFHG 223

Query: 222 --------------------APRRVKWIRMLYTNFSAFAKDQERLISFNERQQANALDYL 281
                               AP+RV+WIR+LY++F  F +DQE LIS + + +    DY+
Sbjct: 224 VLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLK---FDYV 283

Query: 282 EGLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEV 341
           EG V++     + WRSSFF P +  +I S+++  S++YCLE+ K Y       +D+++E+
Sbjct: 284 EGFVIVDEGLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQEVEI 343

Query: 342 LFEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDV 401
           L + L++     F  D+ YV FL+RV   ELKL+S+ LW VPHPWLNLFVPKSRI DFD 
Sbjct: 344 LMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISDFDK 403

Query: 402 GVFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIPDEEVFYTIGFLHSSGFDDWKS--- 461
           GVFK I+   N T GP+LIYPMN  KWD R+S V PDEEVFY +  L S+  D  ++   
Sbjct: 404 GVFKGIL--GNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQKL 463

Query: 462 --FDRQNDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSP 480
                QN  ILEFCE++ I +KQYLP++ TQ +W+ HFG KW  FR +K +FDP++IL+ 
Sbjct: 464 EYLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHILAT 517

[Close]

BLAST of Carg18630 vs. TAIR 10
Match: AT2G19500.1 (cytokinin oxidase 2 )

HSP 1 Score: 421.4 bits (1082), Expect = 9.8e-118
Identity = 225/482 (46.68%), Postives = 288/482 (59.75%), Query Frame = 0


Query: 41  DPESLTLASSDYGNMVKEIPAAVLQPTSINDVVHLINYSYNNPIPFPVAARGQGHSVRGQ 100
           DP  ++ AS D+GN+    P  V+ P+S  D+  L+ Y+ N    F VAARGQGHS+ GQ
Sbjct: 38  DPSIISAASHDFGNITTVTPGGVICPSSTADISRLLQYAANGKSTFQVAARGQGHSLNGQ 97

Query: 101 AMAKNGVVIDMSYLRRNRETPGIIISRLRSTGEIYADVGGEQLWIDVLNAALAYGVTPVS 160
           A    GV+++M+ +        +++S+ +     YADV    LW+DVL      GV+PVS
Sbjct: 98  ASVSGGVIVNMTCI------TDVVVSKDKK----YADVAAGTLWVDVLKKTAEKGVSPVS 157

Query: 161 WTDYLYITVGGTLSNGGISGQSFRYGPQVSNVVEMDVVT--------------------- 220
           WTDYL+ITVGGTLSNGGI GQ FR GP VSNV+E+DV+T                     
Sbjct: 158 WTDYLHITVGGTLSNGGIGGQVFRNGPLVSNVLELDVITGKGEMLTCSRQLNPELFYGVL 217

Query: 221 ------------------APRRVKWIRMLYTNFSAFAKDQERLISFNERQQAN--ALDYL 280
                             AP+R KW RMLY++F+ F KDQERLIS      AN   +DYL
Sbjct: 218 GGLGQFGIITRARIVLDHAPKRAKWFRMLYSDFTTFTKDQERLIS-----MANDIGVDYL 277

Query: 281 EGLVLLHHDSPDTWRSSFFPPSDHPRIISLANQNSLIYCLEVAKYYDRHTQSTIDKDIEV 340
           EG + L +   DT   SFFPPSD  ++  L  Q+ +IY LEVAKYYD      I K I+ 
Sbjct: 278 EGQIFLSNGVVDT---SFFPPSDQSKVADLVKQHGIIYVLEVAKYYDDPNLPIISKVIDT 337

Query: 341 LFEGLDYESGFKFEKDVTYVKFLNRVRSGELKLQSQGLWNVPHPWLNLFVPKSRILDFDV 400
           L + L Y  GF    DV Y  FLNRV   E KL+S GLW +PHPWLNL+VPKSRILDF  
Sbjct: 338 LTKTLSYLPGFISMHDVAYFDFLNRVHVEENKLRSLGLWELPHPWLNLYVPKSRILDFHN 397

Query: 401 GVFKDIVLRRNITKGPVLIYPMNHSKWDSRNSTVIP--DEEVFYTIGFLHSSGFDDWKSF 460
           GV KDI+L++    G  L+YP N +KWD+R S +IP  DE+V Y IG L S+   D    
Sbjct: 398 GVVKDILLKQKSASGLALLYPTNRNKWDNRMSAMIPEIDEDVIYIIGLLQSATPKDLPEV 457

Query: 461 DRQNDEILEFCEKSGIEIKQYLPNYKTQRQWIDHFGSKWTNFRDMKMKFDPKYILSPGQR 480
           +  N++I+ FC+ SGI+IKQYL +Y ++  WI+HFGSKW +F   K  FDPK +LSPGQ 
Sbjct: 458 ESVNEKIIRFCKDSGIKIKQYLMHYTSKEDWIEHFGSKWDDFSKRKDLFDPKKLLSPGQD 501

The following BLAST results are available for this feature:

BLAST of Carg18630 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021337.1	3.2e-288	100.00	Cytokinin dehydrogenase 3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022938074.1	6.1e-279	90.77	cytokinin dehydrogenase 3-like [Cucurbita moschata]	[more]
XP_022965677.1	5.9e-274	89.62	cytokinin dehydrogenase 3-like [Cucurbita maxima]	[more]
KAG6586478.1	2.6e-261	95.69	Cytokinin dehydrogenase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023538489.1	2.3e-241	81.15	cytokinin dehydrogenase 3-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg18630 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LTS3	9.2e-145	50.20	Cytokinin dehydrogenase 3 OS=Arabidopsis thaliana OX=3702 GN=CKX3 PE=1 SV=1	[more]
Q9FUJ2	1.3e-122	45.51	Cytokinin dehydrogenase 4 OS=Arabidopsis thaliana OX=3702 GN=CKX4 PE=1 SV=2	[more]
Q67YU0	7.1e-121	46.07	Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1	[more]
Q5ZAY9	4.3e-118	45.68	Cytokinin dehydrogenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX5 PE=2 ...	[more]
Q9FUJ3	1.4e-116	46.68	Cytokinin dehydrogenase 2 OS=Arabidopsis thaliana OX=3702 GN=CKX2 PE=1 SV=2	[more]

BLAST of Carg18630 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FD08	2.9e-279	90.77	Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111444271 PE=3 SV=1	[more]
A0A6J1HKZ4	2.8e-274	89.62	Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111465505 PE=3 SV=1	[more]
A0A1S3C9Z6	4.9e-234	76.29	Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498521 PE=3 SV=1	[more]
A0A6J1G4H4	2.1e-232	75.86	Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111450699 PE=3 SV=1	[more]
A0A6J1DPL8	4.9e-226	74.57	Cytokinin dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111021923 PE=3 SV=1	[more]

BLAST of Carg18630 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56970.1	6.5e-146	50.20	cytokinin oxidase 3	[more]
AT4G29740.2	9.1e-124	45.51	cytokinin oxidase 4	[more]
AT1G75450.1	5.0e-122	46.07	cytokinin oxidase 5	[more]
AT1G75450.2	6.6e-122	45.98	cytokinin oxidase 5	[more]
AT2G19500.1	9.8e-118	46.68	cytokinin oxidase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 111..199 e-value: 2.1E-62 score: 213.4
IPR016167	FAD-binding, type PCMH, subdomain 1	GENE3D	3.30.43.10		coord: 41..110 e-value: 2.1E-62 score: 213.4 coord: 439..479 e-value: 2.4E-101 score: 341.8
IPR015345	Cytokinin dehydrogenase 1, FAD/cytokinin binding domain	PFAM	PF09265	Cytokin-bind	coord: 201..479 e-value: 3.8E-115 score: 384.1
IPR016170	Cytokinin dehydrogenase, C-terminal domain superfamily	GENE3D	3.40.462.10		coord: 203..438 e-value: 2.4E-101 score: 341.8
IPR006094	FAD linked oxidase, N-terminal	PFAM	PF01565	FAD_binding_4	coord: 60..201 e-value: 5.7E-19 score: 68.2
None	No IPR available	PANTHER	PTHR13878:SF115	CYTOKININ DEHYDROGENASE 1, FAD AND CYTOKININ-BINDING PROTEIN	coord: 199..480
None	No IPR available	PANTHER	PTHR13878	GULONOLACTONE OXIDASE	coord: 199..480 coord: 9..199
None	No IPR available	PANTHER	PTHR13878:SF115	CYTOKININ DEHYDROGENASE 1, FAD AND CYTOKININ-BINDING PROTEIN	coord: 9..199
IPR006093	Oxygen oxidoreductase covalent FAD-binding site	PROSITE	PS00862	OX2_COVAL_FAD	coord: 60..95
IPR016166	FAD-binding domain, PCMH-type	PROSITE	PS51387	FAD_PCMH	coord: 56..249 score: 10.875772
IPR016164	FAD-linked oxidase-like, C-terminal	SUPERFAMILY	55103	FAD-linked oxidases, C-terminal domain	coord: 201..480
IPR036318	FAD-binding, type PCMH-like superfamily	SUPERFAMILY	56176	FAD-binding/transporter-associated domain-like	coord: 35..201

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18630-RA	Carg18630-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009690	cytokinin metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0019139	cytokinin dehydrogenase activity
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016491	oxidoreductase activity