Carg18608 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18608
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	mitochondrial-processing peptidase subunit alpha-like
Location	Carg_Chr12: 11804763 .. 11808394 (-)
RNA-Seq Expression	Carg18608
Synteny	Carg18608

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATAATGCTGATAATTTTGCTTTCGAACATGAAACAATGGGCTGTATGGATTTGCCAGCTCTAGTGCTGTTGCCTCAAACGTACAAATCTTCTGGGGGTCTCTTTGGCTGGTTGCTTCGAGACCGTTCTATATCATCTCCACTAGACTTTCCCCTGTCGGATGTGAATCTTCCGTCTCCATTGCCAGATTATGTTCAACCTAGTAAGACGAAAATCTCAACTCTGCCAAATGGTGTAAGAGTAGCTTCAGAAACATCACCGGTGCACCCATGTGCACCCATAGATTTGGTTTTTTCAAAATCTGTCCATGTGCACCCATATAATTAATGATTCATTACGTTTATTTATTAACATGCTGTTGGTTATTTTGGTGCCTTGGCAAATTCTCTTTTAGCCCCCGAGTCTGCTATAAATAGTCTGAGTGGTACAGTTTTGGAGAAATTTGTTGCTGTAAGTTGAGTAGGAGATCATAATATCATATTTATTTTTAGAATTAATTATTTGATGATGTTACTTCTTTATTGGTCATGCTTCTGCCTTCAGGAAAATTATACTGCAACTCGGATATTACTTGCAGCATCTGGCGTTGAACACGAGGAACTCTTATCCATTGCTGAACCACTTCTATCTGACCTTCGAAGTGTTCCTCGTGAGGTGCCAAAATCAGTGTACAATGGGGGCGATTATCGTCATCAAGGTGATTCTGGGGTGTGTATCATCTATATTGTATACTCTAGCATCCAGCCTTCATTTCCGGGCATCATTCGTTAACTGTTCTCTTTCAGGACGGGAGGACACATTTTGCTCTTGCATTTGAACTCCCACGCGGCTGGCATAAGGAGAAGGATGCTACGGCCTCAACTGTTCTTCAGGTCTTAACAATGGATATCTATTGTGCTTAAGTGATTTTTATGACATGGGGGTTTAGATCATACACCATTAGTTGTTTAGTCCATTTTGCAATTTTTTCACTGTGAAAAGACAAAGGCAATAATGTATCTCATCTCTTAGATGCTACTGGGAGGTGGAGGGTCCTTTTCAGCTGGTGGACCTGGAAAGAGGATGAGCTCTCGGCTGTGTAAGTCCTAAATTTCTTTGACTTTGACGGTCATGCATCTGAGATATTTTTCTTTTCTTGGGGTGGGGTTTGAAGGGAGGTGGAGGGTCTTTTTCAGCTGGTGGACCTGGAAAGGGGATGAGCTCTCGGCCGTGTAAGTCCTAAATTTCTTTGACTTTGACGGCCATACATCTGAGATATTTTTCTTCTCTTGGGTGGTGGGGCTGGGGTTTGAAGGGAGGAATTTGTATTTCCTATACTCGCATGACAAAATATTGTCCTTCACGCCTGCCAAGTCAGGTCACATAGATAGTTTATTATGCAATTATTTCTAGACAGACATAACCATCTTCTGCATGTATATTTCTTAATTTACGCATATTGTATGTTTTCTAGTGTTAACTTCTTTGCAATTATCGATGTGGTAAGCATATCTTCAGGTCTTAAATGAGTATCCACAGGTCCAATCGATTTCAGCATTCAGCAGTATTTACAATAACACTGGCATATTTGGCATCCAAGCAACTACTGTAATGATTATCTTTGGATTGATATTTAACATGATATAGTGCAGAATCACTATGTGGATCTTGATGGTAGAATTGTTAGGTTGATGATATACTTTTTTGAGGGTTCTAATTTCGTGCCAAAATCCTTCCATATTGCAGCTAGTGAACTGTTAGCCATTGCAACGCCTGAAAAAGGTCATTTGTGATTCTCTCTTCTCTAGCATTTCCACAAGAACCATTTCATCTAGCTCACTGATAACATTTCCCTGTTTACAGTTGAACAGGGGCAGCTAGATCGTGCCAAATAGTCAATGAACTCATCTGTGCTAATGAATTTGGAATCCAGGGTATGTATCATTGTACTTTCTTCTCGTTGGCCTTACCATGCCTATATCTTAGGTTGATGAAATAAAAATAATACAGTTTTGAAAATGTTAATCTTTGAGAATGTGTCCACTGATTGTGGTTGTGGAATTGTATCTTGTAGGTGGTTGCTTCAGAGGACATATAGGTCAACTAGTTTTGACATATGGTGAAGGGTACGGTCTGAATCATTTATTGTAGTTTTAATCTGATGCACCATGGCCCATTCATTTCTAATTCATTTCTCTTGTACTAGGAGGGTAAATCTATTTCAAAGATCTGAGATTTAGTTATGCTGTTTTCCCTCATTGAACTGCATTCTGTTACATGATCATTTGTTCACATTTTCTAAATGATATATATCATGCCTCCTGGTTACGCACTTACACTCTAGGGATTCTGGCCTTAGTTAAGAGTGTTAATTTCTTAAGATCTGTTAGAAGTTGCAAATATAATGTGCAGTGATTATCTTTGAATTTTGAGGTGAGCTTTGTAGTTACACCAGCGATCTGTCTTCAAGCCACGCATGCTCATTTTTAATGATCATTTGTTATGTCTTAAATATATAATGTATCCCATTATCCAGGAAACCCGTGGAACATTTCTTGAAAGCTGCGGACGCTGTAACTCTGGAGGATATAGCTTCAATTGCACAAAAACTTCTCTCTTCTCCATTGACAATCGCCTACTTATCATGCTCAATTAGAACCTCATGATATCTGTTTATCTTGCTTCCTCCCGGAAAAAAGAACTGTTTTATTAGCTCTCGATATAACTATTTATTTATATACTGTTCTGCAGTTAGGCATGTTCCAAGCTATGATTCTGTCAGCAGCAAGTTTAAGTCAAAGTGACCAATCGCTTCTTTTGAATCAAACTAGTCTGGCAGACTGGCATCGAACATAGGAATTGCTGTCTTCAAGCCTTGTACGGTCACTCTGATTTCTGAAAACAAGGAAAAGCATTAAGGTCCAGTTTATCTTTCTCAATAATTAGTACATTTGAGCAAGCACCTTTTTGTTAATACCTGATCTCTGTTGATGCCATGTTTTATTCCTTCTATCTAGCAGTGATGTATTTTCAGTTAAAACAGTTTTATCTTGAAGAAAGTTCATGTTCTGAAAGCAATTTCAGGAAGTTTGTATTTTGGAGAAATATTCTTACTGAACATTGTAATTTTCCATGTCTCAAGAGCAACAATCTGAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTATATCTCAACAATAAAGTGTTATTTGCAACACATGCGTGAGCTTTTGAGCATTTTAAATTACGTCAGTTTTTTTTAAAGTATAGTTAGAGTAAGGTGGGGACCTACTTTGTTAGTTTGAACGGTCAATTTTAGATATGCTTTTAGACACACAATTAACTTTAGAGAAATTGCAACTTTGTGGCACAAGATTTGAGTTTAGTGCAACGTTTATTCGTTATTCAGAGCTTTTCTTCTTTTCCTTTCTTTTCTCTTCTTTTCATTTCTTTTTTAATTTTCAGTTACTCAGATCTCTGCTAAAGGATTGGTTGGATACATACGAGGTATTATTCTCCAAAGGTACCTGTACGAATTGCTTGAAAATGTGTATATTTCTTCTGAAAGATGCCTCCAACATCTAACTTCAAGATGATGGATATGATTCATGCATAATCTAAAACTACTAATTATATTATTATTCT

mRNA sequence

ATGGATAATGCTGATAATTTTGCTTTCGAACATGAAACAATGGGCTGTATGGATTTGCCAGCTCTAGTGCTGTTGCCTCAAACGTACAAATCTTCTGGGGGTCTCTTTGGCTGGTTGCTTCGAGACCGTTCTATATCATCTCCACTAGACTTTCCCCTGTCGGATGTGAATCTTCCGTCTCCATTGCCAGATTATGTTCAACCTAGTAAGACGAAAATCTCAACTCTGCCAAATGGTGTAAGAGTAGCTTCAGAAACATCACCGGTGCACCCATGTGCACCCATAGATTTGGAAAATTATACTGCAACTCGGATATTACTTGCAGCATCTGGCGTTGAACACGAGGAACTCTTATCCATTGCTGAACCACTTCTATCTGACCTTCGAAGTGTTCCTCGTGAGGTGCCAAAATCAGTGTACAATGGGGGCGATTATCGTCATCAAGGTGATTCTGGGGACGGGAGGACACATTTTGCTCTTGCATTTGAACTCCCACGCGGCTGGCATAAGGAGAAGGATGCTACGGCCTCAACTGTTCTTCAGATGCTACTGGGAGGTGGAGGGTCCTTTTCAGCTGGTGGACCTGGAAAGAGGATGAGCTCTCGGCTGTCATATCTTCAGGTCTTAAATGAGTATCCACAGGTCCAATCGATTTCAGCATTCAGCAGTATTTACAATAACACTGGCATATTTGGCATCCAAGCAACTACTAATCACTATGTGGATCTTGATGGTAGAATTGTTAGGTTGATGATATACTTTTTTGAGGGTTCTAATTTCGTGCCAAAATCCTTCCATATTGCAGCTAGTGAACTGTTAGCCATTGCAACGCCTGAAAAAGGGGCAGCTAGATCGTGCCAAATAGTCAATGAACTCATCTGTGCTAATGAATTTGGAATCCAGGGTGGTTGCTTCAGAGGACATATAGGTCAACTAGTTTTGACATATGGTGAAGGGAAACCCGTGGAACATTTCTTGAAAGCTGCGGACGCTTTAGGCATGTTCCAAGCTATGATTCTGTCAGCAGCAAGTTTAAGTCAAATTACTCAGATCTCTGCTAAAGGATTGGTTGGATACATACGAGGTATTATTCTCCAAAGGTACCTGTACGAATTGCTTGAAAATGTGTATATTTCTTCTGAAAGATGCCTCCAACATCTAACTTCAAGATGATGGATATGATTCATGCATAATCTAAAACTACTAATTATATTATTATTCT

Coding sequence (CDS)

ATGGATAATGCTGATAATTTTGCTTTCGAACATGAAACAATGGGCTGTATGGATTTGCCAGCTCTAGTGCTGTTGCCTCAAACGTACAAATCTTCTGGGGGTCTCTTTGGCTGGTTGCTTCGAGACCGTTCTATATCATCTCCACTAGACTTTCCCCTGTCGGATGTGAATCTTCCGTCTCCATTGCCAGATTATGTTCAACCTAGTAAGACGAAAATCTCAACTCTGCCAAATGGTGTAAGAGTAGCTTCAGAAACATCACCGGTGCACCCATGTGCACCCATAGATTTGGAAAATTATACTGCAACTCGGATATTACTTGCAGCATCTGGCGTTGAACACGAGGAACTCTTATCCATTGCTGAACCACTTCTATCTGACCTTCGAAGTGTTCCTCGTGAGGTGCCAAAATCAGTGTACAATGGGGGCGATTATCGTCATCAAGGTGATTCTGGGGACGGGAGGACACATTTTGCTCTTGCATTTGAACTCCCACGCGGCTGGCATAAGGAGAAGGATGCTACGGCCTCAACTGTTCTTCAGATGCTACTGGGAGGTGGAGGGTCCTTTTCAGCTGGTGGACCTGGAAAGAGGATGAGCTCTCGGCTGTCATATCTTCAGGTCTTAAATGAGTATCCACAGGTCCAATCGATTTCAGCATTCAGCAGTATTTACAATAACACTGGCATATTTGGCATCCAAGCAACTACTAATCACTATGTGGATCTTGATGGTAGAATTGTTAGGTTGATGATATACTTTTTTGAGGGTTCTAATTTCGTGCCAAAATCCTTCCATATTGCAGCTAGTGAACTGTTAGCCATTGCAACGCCTGAAAAAGGGGCAGCTAGATCGTGCCAAATAGTCAATGAACTCATCTGTGCTAATGAATTTGGAATCCAGGGTGGTTGCTTCAGAGGACATATAGGTCAACTAGTTTTGACATATGGTGAAGGGAAACCCGTGGAACATTTCTTGAAAGCTGCGGACGCTTTAGGCATGTTCCAAGCTATGATTCTGTCAGCAGCAAGTTTAAGTCAAATTACTCAGATCTCTGCTAAAGGATTGGTTGGATACATACGAGGTATTATTCTCCAAAGGTACCTGTACGAATTGCTTGAAAATGTGTATATTTCTTCTGAAAGATGCCTCCAACATCTAACTTCAAGATGA

Protein sequence

MDNADNFAFEHETMGCMDLPALVLLPQTYKSSGGLFGWLLRDRSISSPLDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASETSPVHPCAPIDLENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVLQMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTNHYVDLDGRIVRLMIYFFEGSNFVPKSFHIAASELLAIATPEKGAARSCQIVNELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEHFLKAADALGMFQAMILSAASLSQITQISAKGLVGYIRGIILQRYLYELLENVYISSERCLQHLTSR

Homology

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BLAST of Carg18608 vs. NCBI nr
Match: KAG7021316.1 (Mitochondrial-processing peptidase subunit alpha, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 780.0 bits (2013), Expect = 9.4e-222
Identity = 390/390 (100.00%), Postives = 390/390 (100.00%), Query Frame = 0


Query: 1   MDNADNFAFEHETMGCMDLPALVLLPQTYKSSGGLFGWLLRDRSISSPLDFPLSDVNLPS 60
           MDNADNFAFEHETMGCMDLPALVLLPQTYKSSGGLFGWLLRDRSISSPLDFPLSDVNLPS
Sbjct: 1   MDNADNFAFEHETMGCMDLPALVLLPQTYKSSGGLFGWLLRDRSISSPLDFPLSDVNLPS 60

Query: 61  PLPDYVQPSKTKISTLPNGVRVASETSPVHPCAPIDLENYTATRILLAASGVEHEELLSI 120
           PLPDYVQPSKTKISTLPNGVRVASETSPVHPCAPIDLENYTATRILLAASGVEHEELLSI
Sbjct: 61  PLPDYVQPSKTKISTLPNGVRVASETSPVHPCAPIDLENYTATRILLAASGVEHEELLSI 120

Query: 121 AEPLLSDLRSVPREVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVL 180
           AEPLLSDLRSVPREVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVL
Sbjct: 121 AEPLLSDLRSVPREVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVL 180

Query: 181 QMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTNHY 240
           QMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTNHY
Sbjct: 181 QMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTNHY 240

Query: 241 VDLDGRIVRLMIYFFEGSNFVPKSFHIAASELLAIATPEKGAARSCQIVNELICANEFGI 300
           VDLDGRIVRLMIYFFEGSNFVPKSFHIAASELLAIATPEKGAARSCQIVNELICANEFGI
Sbjct: 241 VDLDGRIVRLMIYFFEGSNFVPKSFHIAASELLAIATPEKGAARSCQIVNELICANEFGI 300

Query: 301 QGGCFRGHIGQLVLTYGEGKPVEHFLKAADALGMFQAMILSAASLSQITQISAKGLVGYI 360
           QGGCFRGHIGQLVLTYGEGKPVEHFLKAADALGMFQAMILSAASLSQITQISAKGLVGYI
Sbjct: 301 QGGCFRGHIGQLVLTYGEGKPVEHFLKAADALGMFQAMILSAASLSQITQISAKGLVGYI 360

Query: 361 RGIILQRYLYELLENVYISSERCLQHLTSR 391
           RGIILQRYLYELLENVYISSERCLQHLTSR
Sbjct: 361 RGIILQRYLYELLENVYISSERCLQHLTSR 390

[Close]

BLAST of Carg18608 vs. NCBI nr
Match: KAG6586461.1 (Mitochondrial-processing peptidase subunit alpha, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 475.7 bits (1223), Expect = 3.8e-130
Identity = 252/320 (78.75%), Postives = 258/320 (80.62%), Query Frame = 0


Query: 14  MGCMDLPALVLLPQTYKSSGGLFGWLLRDRSISSPLDFPLSDVNLPSPLPDYVQPS-KTK 73
           MGCMDLPALVLLPQTYKSSGGLFGWLLRDRSISSPLDFPLSDVNLPSPLPDYVQP+ ++ 
Sbjct: 1   MGCMDLPALVLLPQTYKSSGGLFGWLLRDRSISSPLDFPLSDVNLPSPLPDYVQPTPESA 60

Query: 74  ISTLPNGVRVASETSPVHPCAPIDLENYTATRILLAASGVEHEELLSIAEPLLSDLRSVP 133
           I++L   V                 ENYTATRILLAASGVEHEELLSIAEPLLSDLRSVP
Sbjct: 61  INSLSGTV-----------LEKFVAENYTATRILLAASGVEHEELLSIAEPLLSDLRSVP 120

Query: 134 REVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVLQMLLGGGGSFSA 193
           REVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVLQ   GGGGSFSA
Sbjct: 121 REVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVLQ---GGGGSFSA 180

Query: 194 GGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTNHYVDLDGRIVRLMI 253
           GGPGK MSSR                                   NHYVDLDGRIVRLMI
Sbjct: 181 GGPGKGMSSR--------------------------------PLQNHYVDLDGRIVRLMI 240

Query: 254 YFFEGSNFVPKSFHIAASELLAIATPEKGAARSCQIVNELICANEFGIQGGCFRGHIGQL 313
           YFFEGSNFVPK FHIAASELLAIATPEKGAARSCQIVNELICANEFGIQGGCFRGHIGQL
Sbjct: 241 YFFEGSNFVPKCFHIAASELLAIATPEKGAARSCQIVNELICANEFGIQGGCFRGHIGQL 274

Query: 314 VLTYGEGKPVEHFLKAADAL 333
           VLTYGEGKPVEHFLKAADA+
Sbjct: 301 VLTYGEGKPVEHFLKAADAV 274

[Close]

BLAST of Carg18608 vs. NCBI nr
Match: XP_022946646.1 (mitochondrial-processing peptidase subunit alpha-like [Cucurbita moschata])

HSP 1 Score: 342.0 bits (876), Expect = 6.6e-90
Identity = 226/486 (46.50%), Postives = 250/486 (51.44%), Query Frame = 0


Query: 29  YKSSGGLFGWLLRDRSISSPLDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASETSP 88
           +KSSGGLFGWLL DRS   PLDFPLSDVNLP PLPDYV+P KTKI+TLPNGV+VASETS 
Sbjct: 33  HKSSGGLFGWLLGDRSTLPPLDFPLSDVNLPPPLPDYVEPGKTKITTLPNGVKVASETSS 92

Query: 89  VHPCAPIDL--------------------------------------------------- 148
            +P A I L                                                   
Sbjct: 93  -NPAASIGLYVDCGSIYETPESFGSTHLLERMAFKSTRNRSHLRVVREVEAIGGNVLASA 152

Query: 149 ------------------------------------------------------------ 208
                                                                       
Sbjct: 153 AREQMGYTFNALKSYVPEMVELLIDCVRNPVFLDWEVKEQISRVKDEIIEASNNPHGLLV 212

Query: 209 ------------------------------------ENYTATRILLAASGVEHEELLSIA 268
                                               ENYTA+RI+LAASGVEHEELLSIA
Sbjct: 213 ESIHAAGYSGALANSLLATESAINSLSGPILEKFVSENYTASRIVLAASGVEHEELLSIA 272

Query: 269 EPLLSDLRSVPREVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVLQ 328
           EPLLSDL SVP E PKSVYNGGDYRHQGDSGDGRTHFALAFELP GWHKEKDA A T+LQ
Sbjct: 273 EPLLSDLPSVPHEEPKSVYNGGDYRHQGDSGDGRTHFALAFELPGGWHKEKDAMALTILQ 332

Query: 329 MLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTNHYV 356
           MLLGGGGSFSAGGPGK M SRL YLQVLNEYPQVQSISAFSSIYNN+GIFGIQATT    
Sbjct: 333 MLLGGGGSFSAGGPGKGMYSRL-YLQVLNEYPQVQSISAFSSIYNNSGIFGIQATT---- 392

[Close]

BLAST of Carg18608 vs. NCBI nr
Match: KAG7030470.1 (Mitochondrial-processing peptidase subunit alpha [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 340.9 bits (873), Expect = 1.5e-89
Identity = 225/486 (46.30%), Postives = 250/486 (51.44%), Query Frame = 0


Query: 29  YKSSGGLFGWLLRDRSISSPLDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASETSP 88
           +KSSGGLFGWLL DRS   PL+FPLSDVNLP PLPDYV+P KTKI+TLPNGV+VASETS 
Sbjct: 33  HKSSGGLFGWLLGDRSTLPPLEFPLSDVNLPPPLPDYVEPGKTKITTLPNGVKVASETSS 92

Query: 89  VHPCAPIDL--------------------------------------------------- 148
            +P A I L                                                   
Sbjct: 93  -NPAASIGLYVDCGSIYETPESFGSTHLLERMAFKSTRNRSHLRVVREVEAIGGNVLASA 152

Query: 149 ------------------------------------------------------------ 208
                                                                       
Sbjct: 153 AREQMGYTFNALKSYVPEMVELLIDCVRNPVFLDWEVKEQISRVKDEIIEASNNPHGLLL 212

Query: 209 ------------------------------------ENYTATRILLAASGVEHEELLSIA 268
                                               ENYTA+RI+LAASGVEHEELLSIA
Sbjct: 213 ESIHAAGYSGALANSLLATESAINSLSGPILEKFVSENYTASRIVLAASGVEHEELLSIA 272

Query: 269 EPLLSDLRSVPREVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVLQ 328
           EPLLSDL SVP E PKSVYNGGDYRHQGDSGDGRTHFALAFELP GWHKEKDA A T+LQ
Sbjct: 273 EPLLSDLPSVPHEEPKSVYNGGDYRHQGDSGDGRTHFALAFELPGGWHKEKDAMALTILQ 332

Query: 329 MLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTNHYV 356
           MLLGGGGSFSAGGPGK M SRL YLQVLNEYPQVQSISAFSSIYNN+GIFGIQATT    
Sbjct: 333 MLLGGGGSFSAGGPGKGMYSRL-YLQVLNEYPQVQSISAFSSIYNNSGIFGIQATT---- 392

[Close]

BLAST of Carg18608 vs. NCBI nr
Match: KAG6599494.1 (Mitochondrial-processing peptidase subunit alpha, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 340.9 bits (873), Expect = 1.5e-89
Identity = 225/486 (46.30%), Postives = 250/486 (51.44%), Query Frame = 0


Query: 29  YKSSGGLFGWLLRDRSISSPLDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASETSP 88
           +KSSGGLFGWLL DRS   PL+FPLSDVNLP PLPDYV+P KTKI+TLPNGV+VASETS 
Sbjct: 33  HKSSGGLFGWLLGDRSTLPPLEFPLSDVNLPPPLPDYVEPGKTKITTLPNGVKVASETSS 92

Query: 89  VHPCAPIDL--------------------------------------------------- 148
            +P A I L                                                   
Sbjct: 93  -NPAASIGLYVDCGSIYETPESFGSTHLLERMAFKSTRNRSHLRVVREVEAIGGNVLASA 152

Query: 149 ------------------------------------------------------------ 208
                                                                       
Sbjct: 153 AREQMGYTFNALKSYVPEMVELLIDCVRNPVFLDWEVKEQISRVKDEIIEASNNPHGLLV 212

Query: 209 ------------------------------------ENYTATRILLAASGVEHEELLSIA 268
                                               ENYTA+RI+LAASGVEHEELLSIA
Sbjct: 213 ESIHAAGYSGALANSLLATESAINSLSGPILEKFVSENYTASRIVLAASGVEHEELLSIA 272

Query: 269 EPLLSDLRSVPREVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVLQ 328
           EPLLSDL SVP E PKSVYNGGDYRHQGDSGDGRTHFALAFELP GWHKEKDA A T+LQ
Sbjct: 273 EPLLSDLPSVPHEEPKSVYNGGDYRHQGDSGDGRTHFALAFELPGGWHKEKDAMALTILQ 332

Query: 329 MLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTNHYV 356
           MLLGGGGSFSAGGPGK M SRL YLQVLNEYPQVQSISAFSSIYNN+GIFGIQATT    
Sbjct: 333 MLLGGGGSFSAGGPGKGMYSRL-YLQVLNEYPQVQSISAFSSIYNNSGIFGIQATT---- 392

[Close]

BLAST of Carg18608 vs. ExPASy Swiss-Prot
Match: P29677 (Mitochondrial-processing peptidase subunit alpha OS=Solanum tuberosum OX=4113 GN=MPP PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.9e-61
Identity = 174/464 (37.50%), Postives = 212/464 (45.69%), Query Frame = 0


Query: 30  KSSGGLFGWLLRDRSIS-SPLDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASETSP 89
           K SGGLF W+  D S S +PLDFPL+DV L  PLPDYV+P+KT+I+TL NG++VASE S 
Sbjct: 33  KPSGGLFSWITGDTSSSVTPLDFPLNDVKLSPPLPDYVEPAKTQITTLANGLKVASEAS- 92

Query: 90  VHPCAPIDL--------------------------------------------------- 149
           V+P A I L                                                   
Sbjct: 93  VNPAASIGLYVDCGSIYETPASYGATHLLERMAFKSTLNRSHLRIVREIEAIGGNVTASA 152

Query: 150 ------------------------------------------------------------ 209
                                                                       
Sbjct: 153 SREHMIYTYDALKTYVPQMVEMLADCVRNPAFLDWEVKEQLEKVKAEISEYSKNPQHLLL 212

Query: 210 ------------------------------------ENYTATRILLAASGVEHEELLSIA 269
                                               ENYTA R++LAASGVEHEE L +A
Sbjct: 213 EAVHSAGYAGPYGNSLMATEATINRLNSTVLEEFVAENYTAPRMVLAASGVEHEEFLKVA 272

Query: 270 EPLLSDLRSVPR-EVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVL 329
           EPLLSDL  V   E PK VY GGDYR Q D+    THFALAFE+P GW  EK++   TVL
Sbjct: 273 EPLLSDLPKVATIEEPKPVYVGGDYRCQADA--EMTHFALAFEVPGGWMSEKESMTLTVL 332

Query: 330 QMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTNHY 333
           QML+GGGGSFSAGGPGK M SRL YL+VLN+YPQ+ + SAFSSIYNNTG+FGIQ TT+  
Sbjct: 333 QMLMGGGGSFSAGGPGKGMYSRL-YLRVLNQYPQIHAFSAFSSIYNNTGLFGIQGTTS-- 392

[Close]

BLAST of Carg18608 vs. ExPASy Swiss-Prot
Match: O04308 (Probable mitochondrial-processing peptidase subunit alpha-2, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MPPalpha2 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 3.8e-48
Identity = 117/248 (47.18%), Postives = 153/248 (61.69%), Query Frame = 0


Query: 98  ENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREV-PKSVYNGGDYRHQGDSGDGRT 157
           ENYTA+R++LAASGV+HEELL + EPLLSDL +VPR   PKS Y GGD+R    +G   T
Sbjct: 247 ENYTASRMVLAASGVDHEELLKVVEPLLSDLPNVPRPAEPKSQYVGGDFRQH--TGGEAT 306

Query: 158 HFALAFELPRGWHKEKDATASTVLQMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQ 217
           HFALAFE+P GW+ EK+A  +TVLQML+GGGGSFSAGGPGK M S L YL++LN++ Q Q
Sbjct: 307 HFALAFEVP-GWNNEKEAIIATVLQMLMGGGGSFSAGGPGKGMHSWL-YLRLLNQHQQFQ 366

Query: 218 SISAFSSIYNNTGIFGIQATTNHYVDLDGRIVRLMIYFFEGSNFVPKSFHIAASELLAIA 277
           S +AF+S++NNTG+FGI   T+                     F  +   + ASE+ A+A
Sbjct: 367 SCTAFTSVFNNTGLFGIYGCTS-------------------PEFASQGIELVASEMNAVA 426

Query: 278 TPE---------KGAARSCQIVN---ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEH 333
             +         K A +S  ++N    +I A +           IG+ +LTYGE KPV+ 
Sbjct: 427 DGKVNQKHLDRAKAATKSAILMNLESRMIAAED-----------IGRQILTYGERKPVDQ 460

HSP 2 Score: 57.4 bits (137), Expect = 4.2e-07
Identity = 32/68 (47.06%), Postives = 44/68 (64.71%), Query Frame = 0


Query: 31 SSGGLFGWLLRDRSISSP-LDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASETSPV 90
          +S     WL    S S P ++ PL+ V+LP PL D+V+PSK K +TLPNG+ +A+E SP 
Sbjct: 33 TSSSSSSWLSGGYSSSLPSMNIPLAGVSLPPPLSDHVEPSKLKTTTLPNGLTIATEMSP- 92

Query: 91 HPCAPIDL 98
          +P A I L
Sbjct: 93 NPAASIGL 99

[Close]

BLAST of Carg18608 vs. ExPASy Swiss-Prot
Match: Q9ZU25 (Probable mitochondrial-processing peptidase subunit alpha-1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MPPalpha1 PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.1e-47
Identity = 118/248 (47.58%), Postives = 153/248 (61.69%), Query Frame = 0


Query: 98  ENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREV-PKSVYNGGDYRHQGDSGDGRT 157
           EN+TA R++LAASGVEHEELL +AEPL SDL +VP ++ PKS Y GGD+R    +G   T
Sbjct: 251 ENFTAARMVLAASGVEHEELLKVAEPLTSDLPNVPPQLAPKSQYVGGDFRQH--TGGEAT 310

Query: 158 HFALAFELPRGWHKEKDATASTVLQMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQ 217
           HFA+AFE+P GW+ EK+A  +TVLQML+GGGGSFSAGGPGK M S L Y +VLNEY +VQ
Sbjct: 311 HFAVAFEVP-GWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWL-YRRVLNEYQEVQ 370

Query: 218 SISAFSSIYNNTGIFGIQATTNHYVDLDGRIVRLMIYFFEGSNFVPKSFHIAASELLAIA 277
           S +AF+SI+N+TG+FG                   IY      F  K+  +AA EL  +A
Sbjct: 371 SCTAFTSIFNDTGLFG-------------------IYGCSSPQFAAKAIELAAKELKDVA 430

Query: 278 TPE---------KGAARSCQIVN---ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEH 333
             +         K A +S  ++N    +I A +           IG+ +LTYGE KPV+ 
Sbjct: 431 GGKVNQAHLDRAKAATKSAVLMNLESRMIAAED-----------IGRQILTYGERKPVDQ 464

HSP 2 Score: 62.8 bits (151), Expect = 1.0e-08
Identity = 34/72 (47.22%), Postives = 47/72 (65.28%), Query Frame = 0


Query: 27  QTYKSSGGLFGWLL-RDRSISSPLDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASE 86
           +T  S+     WL    R+  + LD PL  V+LP PL D V+PSK +I+TLPNG+++ASE
Sbjct: 33  ETSSSTPAYLSWLSGGSRAALTSLDMPLQGVSLPPPLADKVEPSKLQITTLPNGLKIASE 92

Query: 87  TSPVHPCAPIDL 98
           T+P +P A I L
Sbjct: 93  TTP-NPAASIGL 103

[Close]

BLAST of Carg18608 vs. ExPASy Swiss-Prot
Match: P11914 (Mitochondrial-processing peptidase subunit alpha OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MAS2 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.6e-14
Identity = 60/141 (42.55%), Postives = 74/141 (52.48%), Query Frame = 0


Query: 100 YTATRILLAASGVEHEELLSIAEPLLSDLRSVPREVPKSV--YNGGDYRHQGDSGDGRT- 159
           YT    + A  GV HE+ L + E  L D +S    + K V  Y GG+         G   
Sbjct: 195 YTPENTVAAFVGVPHEKALELTEKYLGDWQSTHPPITKKVAQYTGGESCIPPAPVFGNLP 254

Query: 160 ---HFALAFE-LPRGWHKEKDATASTVLQMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEY 219
              H  + FE LP       D  A   LQ LLGGGGSFSAGGPGK M SRL Y  VLN+Y
Sbjct: 255 ELFHIQIGFEGLP---IDHPDIYALATLQTLLGGGGSFSAGGPGKGMYSRL-YTHVLNQY 314

Query: 220 PQVQSISAFSSIYNNTGIFGI 234
             V++  AF+  Y+++GIFGI
Sbjct: 315 YFVENCVAFNHSYSDSGIFGI 331

[Close]

BLAST of Carg18608 vs. ExPASy Swiss-Prot
Match: O94745 (Probable mitochondrial-processing peptidase subunit alpha OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mas2 PE=3 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 1.8e-13
Identity = 59/147 (40.14%), Postives = 74/147 (50.34%), Query Frame = 0


Query: 100 YTATRILLAASGVEHEELLSIAEPLLSDLRS---VPREVPKSVYNGGDYRHQGDSG---- 159
           Y    + LA +G+  E    I + L   L S    P E   S Y GG    +        
Sbjct: 230 YRPEHLTLAYAGIPQEIAKEITKELYGHLPSSSLPPLEAIPSHYTGGFMGIKKSEAPPVP 289

Query: 160 --DGRTHFALAFE-LPRGWHKEKDATASTVLQMLLGGGGSFSAGGPGKRMSSRLSYLQVL 219
                TH  +A E LP     + D  A   LQ LLGGGGSFSAGGPGK M SRL YL VL
Sbjct: 290 YQQEFTHVVIAMEGLP---VTDPDIYALACLQFLLGGGGSFSAGGPGKGMYSRL-YLNVL 349

Query: 220 NEYPQVQSISAFSSIYNNTGIFGIQAT 237
           N+YP V++  AF+  Y ++G+FG+  T
Sbjct: 350 NQYPWVETCMAFNHSYTDSGLFGMFVT 372

[Close]

BLAST of Carg18608 vs. ExPASy TrEMBL
Match: A0A6J1G486 (mitochondrial-processing peptidase subunit alpha-like OS=Cucurbita moschata OX=3662 GN=LOC111450651 PE=3 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 3.2e-90
Identity = 226/486 (46.50%), Postives = 250/486 (51.44%), Query Frame = 0


Query: 29  YKSSGGLFGWLLRDRSISSPLDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASETSP 88
           +KSSGGLFGWLL DRS   PLDFPLSDVNLP PLPDYV+P KTKI+TLPNGV+VASETS 
Sbjct: 33  HKSSGGLFGWLLGDRSTLPPLDFPLSDVNLPPPLPDYVEPGKTKITTLPNGVKVASETSS 92

Query: 89  VHPCAPIDL--------------------------------------------------- 148
            +P A I L                                                   
Sbjct: 93  -NPAASIGLYVDCGSIYETPESFGSTHLLERMAFKSTRNRSHLRVVREVEAIGGNVLASA 152

Query: 149 ------------------------------------------------------------ 208
                                                                       
Sbjct: 153 AREQMGYTFNALKSYVPEMVELLIDCVRNPVFLDWEVKEQISRVKDEIIEASNNPHGLLV 212

Query: 209 ------------------------------------ENYTATRILLAASGVEHEELLSIA 268
                                               ENYTA+RI+LAASGVEHEELLSIA
Sbjct: 213 ESIHAAGYSGALANSLLATESAINSLSGPILEKFVSENYTASRIVLAASGVEHEELLSIA 272

Query: 269 EPLLSDLRSVPREVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVLQ 328
           EPLLSDL SVP E PKSVYNGGDYRHQGDSGDGRTHFALAFELP GWHKEKDA A T+LQ
Sbjct: 273 EPLLSDLPSVPHEEPKSVYNGGDYRHQGDSGDGRTHFALAFELPGGWHKEKDAMALTILQ 332

Query: 329 MLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTNHYV 356
           MLLGGGGSFSAGGPGK M SRL YLQVLNEYPQVQSISAFSSIYNN+GIFGIQATT    
Sbjct: 333 MLLGGGGSFSAGGPGKGMYSRL-YLQVLNEYPQVQSISAFSSIYNNSGIFGIQATT---- 392

[Close]

BLAST of Carg18608 vs. ExPASy TrEMBL
Match: A0A6J1KD66 (mitochondrial-processing peptidase subunit alpha-like OS=Cucurbita maxima OX=3661 GN=LOC111493827 PE=3 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 2.5e-87
Identity = 223/504 (44.25%), Postives = 249/504 (49.40%), Query Frame = 0


Query: 11  HETMGCMDLPALVLLPQTYKSSGGLFGWLLRDRSISSPLDFPLSDVNLPSPLPDYVQPSK 70
           H   G     +   +   +KSSGGL GWLL DRS   PLDFPLSDVNLP PLPDYV+P K
Sbjct: 15  HANNGLYRFASSSAVASKHKSSGGLLGWLLGDRSTLPPLDFPLSDVNLPPPLPDYVEPGK 74

Query: 71  TKISTLPNGVRVASETSPVHPCAPIDL--------------------------------- 130
           TKI+TLPNGV+VASETS  +P A I L                                 
Sbjct: 75  TKITTLPNGVKVASETSS-NPAASIGLYVDCGSIYETPESFGSTHLLERMAFKSTRNRSH 134

Query: 131 ------------------------------------------------------------ 190
                                                                       
Sbjct: 135 LRVVREVEAIGGNVLASAAREQMGYTFNALKSYVPEMVELLIDCVRNPVFLDWEVNEQIS 194

Query: 191 ------------------------------------------------------ENYTAT 250
                                                                 ENYTA+
Sbjct: 195 RAKDEIIEASNNPHGLLLESIHAAGYSGALANSLLATESAINSLSGTILEKFVSENYTAS 254

Query: 251 RILLAASGVEHEELLSIAEPLLSDLRSVPREVPKSVYNGGDYRHQGDSGDGRTHFALAFE 310
           R +LAASGVEHEELLSIAEPLLSDL S P E PKSVYNGGD RHQGDSGDGRTHFALAFE
Sbjct: 255 RTVLAASGVEHEELLSIAEPLLSDLPSAPHEEPKSVYNGGDCRHQGDSGDGRTHFALAFE 314

Query: 311 LPRGWHKEKDATASTVLQMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSS 356
           LP GWHKEKDA A T+LQMLLGGGGSFSAGGPGK M SRL YLQVLNEYPQVQSISAFSS
Sbjct: 315 LPGGWHKEKDAMALTILQMLLGGGGSFSAGGPGKGMYSRL-YLQVLNEYPQVQSISAFSS 374

[Close]

BLAST of Carg18608 vs. ExPASy TrEMBL
Match: A0A0A0LEX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000680 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 7.3e-87
Identity = 220/483 (45.55%), Postives = 248/483 (51.35%), Query Frame = 0


Query: 30  KSSGGLFGWLLRDRSISSPLDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASETSPV 89
           KSSGGLFGWLL DRS   PLDFPLSDV LP PLPDYV+P KTKI++LPNGV+VASETSP 
Sbjct: 34  KSSGGLFGWLLGDRSALPPLDFPLSDVTLPPPLPDYVEPGKTKITSLPNGVKVASETSP- 93

Query: 90  HPCAPIDL---------------------------------------------------- 149
            P A I L                                                    
Sbjct: 94  DPVASIGLYVDCGSSYETPETFGSTHLLERMAFKTTSNRSHLRVVREVEAIGGNVLASDA 153

Query: 150 ------------------------------------------------------------ 209
                                                                       
Sbjct: 154 REQMGYTLNALKSYVPEIVELLVDCVRNPVFLDWEVNEQIIEASNNPHGLLLEAIHAAGY 213

Query: 210 ----------------------------ENYTATRILLAASGVEHEELLSIAEPLLSDLR 269
                                       ENYTA+RI+LAASGVEHEELLSIAEPLLSDL 
Sbjct: 214 SGALANSLVAPESAIHSLSGTILENFVSENYTASRIVLAASGVEHEELLSIAEPLLSDLP 273

Query: 270 SVPREVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVLQMLLGGGGS 329
           SVP + PKSVYNGGDYRHQGDSGDGRTHFALAFELP GW KEKDA A TVLQMLLGGGGS
Sbjct: 274 SVPHQEPKSVYNGGDYRHQGDSGDGRTHFALAFELPGGWRKEKDAMALTVLQMLLGGGGS 333

Query: 330 FSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTNHYVDLDGRIVR 361
           FSAGGPGK M SRL YLQVLNEYPQVQSISAFSSIYNN+G+FGI+ TT            
Sbjct: 334 FSAGGPGKGMYSRL-YLQVLNEYPQVQSISAFSSIYNNSGLFGIKGTT------------ 393

[Close]

BLAST of Carg18608 vs. ExPASy TrEMBL
Match: A0A6J1DL19 (mitochondrial-processing peptidase subunit alpha-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021899 PE=3 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 3.6e-86
Identity = 217/463 (46.87%), Postives = 236/463 (50.97%), Query Frame = 0


Query: 29  YKSSGGLFGWLLRDRSISSPLDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASETSP 88
           + SSGGLFGWL    S   PL+FPLSDVNLP PLPDYV+P KTKI+TLPNGV+VASETS 
Sbjct: 35  HSSSGGLFGWLFGGNSTLPPLEFPLSDVNLPPPLPDYVEPGKTKITTLPNGVKVASETSS 94

Query: 89  VHPCAPIDL--------------------------------------------------- 148
            +P A I L                                                   
Sbjct: 95  -NPAASIGLYVDCGSIYETPETFGSTHLLERMAFKSTRNRSHLRVVREVEAIGGNVLASA 154

Query: 149 ------------------------------------------------------------ 208
                                                                       
Sbjct: 155 AREQMGYTFNALKSYVPEMVELLIDCVRNPVFLDWEVNEQLSRVKDEIVEASNNPHGFLL 214

Query: 209 ------------------------------------ENYTATRILLAASGVEHEELLSIA 268
                                               ENY+A+RI+LAASGVEHEELLSIA
Sbjct: 215 EAVHAAGYSGALANSLLASESAINSLGGPILEKFVSENYSASRIVLAASGVEHEELLSIA 274

Query: 269 EPLLSDLRSVPREVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVLQ 328
           EPLLSDL SVPRE PKSVYNGGDYRHQGDSGDGRTHFALAFELP GW KEKDA A TVLQ
Sbjct: 275 EPLLSDLPSVPREEPKSVYNGGDYRHQGDSGDGRTHFALAFELPGGWRKEKDAMALTVLQ 334

Query: 329 MLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTNHYV 333
           ML+GGGGSFSAGGPGK M SRL YLQVLNEYPQVQSISAFSSIYNNTGIFGIQATT    
Sbjct: 335 MLMGGGGSFSAGGPGKGMYSRL-YLQVLNEYPQVQSISAFSSIYNNTGIFGIQATT---- 394

[Close]

BLAST of Carg18608 vs. ExPASy TrEMBL
Match: A0A5D3CG40 (Mitochondrial-processing peptidase subunit alpha-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00740 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 1.4e-85
Identity = 219/490 (44.69%), Postives = 246/490 (50.20%), Query Frame = 0


Query: 30  KSSGGLFGWLLRDRSISSPLDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASETSPV 89
           KSSGGLFGW L DRS   PLDFPLSDV LP PLPDYV+P KTKI++LPNGVRVASETSP 
Sbjct: 22  KSSGGLFGWFLGDRSTLPPLDFPLSDVTLPPPLPDYVEPGKTKITSLPNGVRVASETSP- 81

Query: 90  HPCAPIDL---------------------------------------------------- 149
            P A I L                                                    
Sbjct: 82  DPAASIGLYVDCGSSYETPETFGSTHLLEHMAFHTTRNRSHLRVVREVEAIGGNVLASAA 141

Query: 150 ------------------------------------------------------------ 209
                                                                       
Sbjct: 142 REQMGYTFSALKSHVPEMVELLIDCVRNPVFLDWEVNEQLSRVKEEITEASNNPHGLLLE 201

Query: 210 -----------------------------------ENYTATRILLAASGVEHEELLSIAE 269
                                              ENYTA+RI+LAASGVEHEELLSIAE
Sbjct: 202 AIHAAGYSGALANSLVAPESAIHSLSGTILENFVSENYTASRIVLAASGVEHEELLSIAE 261

Query: 270 PLLSDLRSVPREVPKSVYNGGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTVLQM 329
           PLLSDL  VP + PKSVYNGGDYRHQGDSGDGRTHFALAFELP GW KEKDA A TVLQM
Sbjct: 262 PLLSDLPGVPHQEPKSVYNGGDYRHQGDSGDGRTHFALAFELPGGWRKEKDAMALTVLQM 321

Query: 330 LLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQATTNHYVD 361
           LLGGGGSFSAGGPGK M SRL YLQVLNEYPQVQSISAFSSIYNN+G+FGI+ TT     
Sbjct: 322 LLGGGGSFSAGGPGKGMYSRL-YLQVLNEYPQVQSISAFSSIYNNSGLFGIKGTT----- 381

[Close]

BLAST of Carg18608 vs. TAIR 10
Match: AT3G16480.1 (mitochondrial processing peptidase alpha subunit )

HSP 1 Score: 193.7 bits (491), Expect = 2.7e-49
Identity = 117/248 (47.18%), Postives = 153/248 (61.69%), Query Frame = 0


Query: 98  ENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREV-PKSVYNGGDYRHQGDSGDGRT 157
           ENYTA+R++LAASGV+HEELL + EPLLSDL +VPR   PKS Y GGD+R    +G   T
Sbjct: 247 ENYTASRMVLAASGVDHEELLKVVEPLLSDLPNVPRPAEPKSQYVGGDFRQH--TGGEAT 306

Query: 158 HFALAFELPRGWHKEKDATASTVLQMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQ 217
           HFALAFE+P GW+ EK+A  +TVLQML+GGGGSFSAGGPGK M S L YL++LN++ Q Q
Sbjct: 307 HFALAFEVP-GWNNEKEAIIATVLQMLMGGGGSFSAGGPGKGMHSWL-YLRLLNQHQQFQ 366

Query: 218 SISAFSSIYNNTGIFGIQATTNHYVDLDGRIVRLMIYFFEGSNFVPKSFHIAASELLAIA 277
           S +AF+S++NNTG+FGI   T+                     F  +   + ASE+ A+A
Sbjct: 367 SCTAFTSVFNNTGLFGIYGCTS-------------------PEFASQGIELVASEMNAVA 426

Query: 278 TPE---------KGAARSCQIVN---ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEH 333
             +         K A +S  ++N    +I A +           IG+ +LTYGE KPV+ 
Sbjct: 427 DGKVNQKHLDRAKAATKSAILMNLESRMIAAED-----------IGRQILTYGERKPVDQ 460

HSP 2 Score: 57.4 bits (137), Expect = 3.0e-08
Identity = 32/68 (47.06%), Postives = 44/68 (64.71%), Query Frame = 0


Query: 31 SSGGLFGWLLRDRSISSP-LDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASETSPV 90
          +S     WL    S S P ++ PL+ V+LP PL D+V+PSK K +TLPNG+ +A+E SP 
Sbjct: 33 TSSSSSSWLSGGYSSSLPSMNIPLAGVSLPPPLSDHVEPSKLKTTTLPNGLTIATEMSP- 92

Query: 91 HPCAPIDL 98
          +P A I L
Sbjct: 93 NPAASIGL 99

[Close]

BLAST of Carg18608 vs. TAIR 10
Match: AT1G51980.1 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 192.2 bits (487), Expect = 7.8e-49
Identity = 118/248 (47.58%), Postives = 153/248 (61.69%), Query Frame = 0


Query: 98  ENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREV-PKSVYNGGDYRHQGDSGDGRT 157
           EN+TA R++LAASGVEHEELL +AEPL SDL +VP ++ PKS Y GGD+R    +G   T
Sbjct: 251 ENFTAARMVLAASGVEHEELLKVAEPLTSDLPNVPPQLAPKSQYVGGDFRQH--TGGEAT 310

Query: 158 HFALAFELPRGWHKEKDATASTVLQMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQ 217
           HFA+AFE+P GW+ EK+A  +TVLQML+GGGGSFSAGGPGK M S L Y +VLNEY +VQ
Sbjct: 311 HFAVAFEVP-GWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWL-YRRVLNEYQEVQ 370

Query: 218 SISAFSSIYNNTGIFGIQATTNHYVDLDGRIVRLMIYFFEGSNFVPKSFHIAASELLAIA 277
           S +AF+SI+N+TG+FG                   IY      F  K+  +AA EL  +A
Sbjct: 371 SCTAFTSIFNDTGLFG-------------------IYGCSSPQFAAKAIELAAKELKDVA 430

Query: 278 TPE---------KGAARSCQIVN---ELICANEFGIQGGCFRGHIGQLVLTYGEGKPVEH 333
             +         K A +S  ++N    +I A +           IG+ +LTYGE KPV+ 
Sbjct: 431 GGKVNQAHLDRAKAATKSAVLMNLESRMIAAED-----------IGRQILTYGERKPVDQ 464

HSP 2 Score: 62.8 bits (151), Expect = 7.2e-10
Identity = 34/72 (47.22%), Postives = 47/72 (65.28%), Query Frame = 0


Query: 27  QTYKSSGGLFGWLL-RDRSISSPLDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASE 86
           +T  S+     WL    R+  + LD PL  V+LP PL D V+PSK +I+TLPNG+++ASE
Sbjct: 33  ETSSSTPAYLSWLSGGSRAALTSLDMPLQGVSLPPPLADKVEPSKLQITTLPNGLKIASE 92

Query: 87  TSPVHPCAPIDL 98
           T+P +P A I L
Sbjct: 93  TTP-NPAASIGL 103

[Close]

BLAST of Carg18608 vs. TAIR 10
Match: AT1G51980.2 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 176.0 bits (445), Expect = 5.8e-44
Identity = 110/234 (47.01%), Postives = 143/234 (61.11%), Query Frame = 0


Query: 98  ENYTATRILLAASGVEHEELLSIAEPLLSDLRSVPREV-PKSVYNGGDYRHQGDSGDGRT 157
           EN+TA R++LAASGVEHEELL +AEPL SDL +VP ++ PKS Y GGD+R    +G   T
Sbjct: 251 ENFTAARMVLAASGVEHEELLKVAEPLTSDLPNVPPQLAPKSQYVGGDFRQH--TGGEAT 310

Query: 158 HFALAFELPRGWHKEKDATASTVLQMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQ 217
           HFA+AFE+P GW+ EK+A  +TVLQML+GGGGSFSAGGPGK M S L Y +VLNEY +VQ
Sbjct: 311 HFAVAFEVP-GWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWL-YRRVLNEYQEVQ 370

Query: 218 SISAFSSIYNNTGIFGIQATTNHYVDLDGRIVRLMIYFFEGSNFVPKSFHIAASELLAIA 277
           S +AF+SI+N+TG+FG                   IY      F  K+  +AA EL  +A
Sbjct: 371 SCTAFTSIFNDTGLFG-------------------IYGCSSPQFAAKAIELAAKELKDVA 430

Query: 278 TPE---------KGAARSCQIVN---ELICANEFGIQGGCFRGHIGQLVLTYGE 319
             +         K A +S  ++N    +I A +           IG+ +LTYGE
Sbjct: 431 GGKVNQAHLDRAKAATKSAVLMNLESRMIAAED-----------IGRQILTYGE 450

HSP 2 Score: 62.8 bits (151), Expect = 7.2e-10
Identity = 34/72 (47.22%), Postives = 47/72 (65.28%), Query Frame = 0


Query: 27  QTYKSSGGLFGWLL-RDRSISSPLDFPLSDVNLPSPLPDYVQPSKTKISTLPNGVRVASE 86
           +T  S+     WL    R+  + LD PL  V+LP PL D V+PSK +I+TLPNG+++ASE
Sbjct: 33  ETSSSTPAYLSWLSGGSRAALTSLDMPLQGVSLPPPLADKVEPSKLQITTLPNGLKIASE 92

Query: 87  TSPVHPCAPIDL 98
           T+P +P A I L
Sbjct: 93  TTP-NPAASIGL 103

[Close]

BLAST of Carg18608 vs. TAIR 10
Match: AT3G02090.1 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 59.7 bits (143), Expect = 6.1e-09
Identity = 56/176 (31.82%), Postives = 84/176 (47.73%), Query Frame = 0


Query: 71  TKISTLPNGVRVASETSPVHPCAPIDLEN-----YTATRILLAASG-VEHEELLSIAEPL 130
           T     P G  +      V      DL+N     YTA+R+++AA+G V+HEE++   + L
Sbjct: 240 TAFQYTPLGRTILGPAQNVKSITREDLQNYIKTHYTASRMVIAAAGAVKHEEVVEQVKKL 299

Query: 131 LSDLRSVPREVPKSVYN-----GGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTV 190
            + L S P    + V N      G      D       FA+AFE    W  + D+ A  V
Sbjct: 300 FTKLSSDPTTTSQLVANEPASFTGSEVRMIDDDLPLAQFAVAFE-GASW-TDPDSVALMV 359

Query: 191 LQMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQA 236
           +Q +LG     + GG GK + S L+    +NE    +SI AF++ Y +TG+FG+ A
Sbjct: 360 MQTMLGSWNK-NVGG-GKHVGSDLTQRVAINEI--AESIMAFNTNYKDTGLFGVYA 409

[Close]

BLAST of Carg18608 vs. TAIR 10
Match: AT3G02090.2 (Insulinase (Peptidase family M16) protein )

HSP 1 Score: 59.7 bits (143), Expect = 6.1e-09
Identity = 56/176 (31.82%), Postives = 84/176 (47.73%), Query Frame = 0


Query: 71  TKISTLPNGVRVASETSPVHPCAPIDLEN-----YTATRILLAASG-VEHEELLSIAEPL 130
           T     P G  +      V      DL+N     YTA+R+++AA+G V+HEE++   + L
Sbjct: 240 TAFQYTPLGRTILGPAQNVKSITREDLQNYIKTHYTASRMVIAAAGAVKHEEVVEQVKKL 299

Query: 131 LSDLRSVPREVPKSVYN-----GGDYRHQGDSGDGRTHFALAFELPRGWHKEKDATASTV 190
            + L S P    + V N      G      D       FA+AFE    W  + D+ A  V
Sbjct: 300 FTKLSSDPTTTSQLVANEPASFTGSEVRMIDDDLPLAQFAVAFE-GASW-TDPDSVALMV 359

Query: 191 LQMLLGGGGSFSAGGPGKRMSSRLSYLQVLNEYPQVQSISAFSSIYNNTGIFGIQA 236
           +Q +LG     + GG GK + S L+    +NE    +SI AF++ Y +TG+FG+ A
Sbjct: 360 MQTMLGSWNK-NVGG-GKHVGSDLTQRVAINEI--AESIMAFNTNYKDTGLFGVYA 409

The following BLAST results are available for this feature:

BLAST of Carg18608 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021316.1	9.4e-222	100.00	Mitochondrial-processing peptidase subunit alpha, partial [Cucurbita argyrosperm...	[more]
KAG6586461.1	3.8e-130	78.75	Mitochondrial-processing peptidase subunit alpha, partial [Cucurbita argyrosperm...	[more]
XP_022946646.1	6.6e-90	46.50	mitochondrial-processing peptidase subunit alpha-like [Cucurbita moschata]	[more]
KAG7030470.1	1.5e-89	46.30	Mitochondrial-processing peptidase subunit alpha [Cucurbita argyrosperma subsp. ...	[more]
KAG6599494.1	1.5e-89	46.30	Mitochondrial-processing peptidase subunit alpha, partial [Cucurbita argyrosperm...	[more]

BLAST of Carg18608 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P29677	3.9e-61	37.50	Mitochondrial-processing peptidase subunit alpha OS=Solanum tuberosum OX=4113 GN...	[more]
O04308	3.8e-48	47.18	Probable mitochondrial-processing peptidase subunit alpha-2, chloroplastic/mitoc...	[more]
Q9ZU25	1.1e-47	47.58	Probable mitochondrial-processing peptidase subunit alpha-1, mitochondrial OS=Ar...	[more]
P11914	1.6e-14	42.55	Mitochondrial-processing peptidase subunit alpha OS=Saccharomyces cerevisiae (st...	[more]
O94745	1.8e-13	40.14	Probable mitochondrial-processing peptidase subunit alpha OS=Schizosaccharomyces...	[more]

BLAST of Carg18608 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G486	3.2e-90	46.50	mitochondrial-processing peptidase subunit alpha-like OS=Cucurbita moschata OX=3...	[more]
A0A6J1KD66	2.5e-87	44.25	mitochondrial-processing peptidase subunit alpha-like OS=Cucurbita maxima OX=366...	[more]
A0A0A0LEX1	7.3e-87	45.55	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000680 PE=3 SV=1	[more]
A0A6J1DL19	3.6e-86	46.87	mitochondrial-processing peptidase subunit alpha-like isoform X1 OS=Momordica ch...	[more]
A0A5D3CG40	1.4e-85	44.69	Mitochondrial-processing peptidase subunit alpha-like OS=Cucumis melo var. makuw...	[more]

BLAST of Carg18608 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G16480.1	2.7e-49	47.18	mitochondrial processing peptidase alpha subunit	[more]
AT1G51980.1	7.8e-49	47.58	Insulinase (Peptidase family M16) protein	[more]
AT1G51980.2	5.8e-44	47.01	Insulinase (Peptidase family M16) protein	[more]
AT3G02090.1	6.1e-09	31.82	Insulinase (Peptidase family M16) protein	[more]
AT3G02090.2	6.1e-09	31.82	Insulinase (Peptidase family M16) protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007863	Peptidase M16, C-terminal	PFAM	PF05193	Peptidase_M16_C	coord: 98..238 e-value: 3.2E-16 score: 59.9
None	No IPR available	GENE3D	3.30.830.10		coord: 138..251 e-value: 1.6E-25 score: 91.9
None	No IPR available	PANTHER	PTHR11851	METALLOPROTEASE	coord: 27..95 coord: 98..359
None	No IPR available	PANTHER	PTHR11851:SF206	PROCESSING PEPTIDASE	coord: 27..95 coord: 98..359
IPR011249	Metalloenzyme, LuxS/M16 peptidase-like	SUPERFAMILY	63411	LuxS/MPP-like metallohydrolase	coord: 139..238

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18608-RA	Carg18608-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0046872	metal ion binding