Carg18601 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg18601
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMitogen-activated protein kinase kinase
LocationCarg_Chr12: 11767614 .. 11770658 (+)
RNA-Seq ExpressionCarg18601
SyntenyCarg18601
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCTCCTCATAGTTTGAATTTAATTCATTCATTTTTTCGCTGCTTTGACGAAGCAGATCGTCGCCTTCTTCACAGAGAAAAGGGGAAGAACCTCAATCGCACGTTTTTCCTCTCATTCTCTTCTTCCAATCTCCTCTATCTGTCTCTTTTAGTTCCTCATCCGCTATTGAAACCCTAGGAATTTGATAAAGACAACATAAAAGATGAAGACCAAGACGCCATTGAAGCAGCTCAAGCTCTCTGTTCCCGCTCAAGAAACTTCTATCCGTTCTTTTCTGTAAGTTCGAATAGTTTTTCTCTTGCTCTCGACTTAATCCTGATCGTTTGTTCTTCGTTTTAAGTGTTCGTTTCGTTTCAGTTCCTTGTAGCTACTATCTGATTGATCCGGTAAAAATATGCTTTCGTGTGTTTTTCAGTGGAGGTTCGATTGAGTTGTAGTCGTAGTTTCTTGGAAATTAACGCTGTAACGAAGAAAAGGATTTTTTAAGTGTGGCGAACTTATCTGTTGTTGAGTGAATAAATTCAAATGACTTGTTTTATTGTTGTACTATCCATTTCAAGTATTTCAAATTTCAGGAAGGGCAAAGGACATGATCTGTTCGATTTATTTTCTTTTTCGGTTTTTGTTGTCCGTTTTCCTTATTTCCGCTTCATCCTCTTTCTGATTTTGTTAAACTTCAGAACTGCGAGCGGAACATTTCACGACGGCAATCTGCTTTTGAACCAGAAAGGATTGCGGCTTGTATCTGAAGAGAAAGAAACGCAGGTAAGTTCGTTTTAAGTTTTTGACGTATATCAATGTGTAATCGGAATTAGTGAAAACTCGCCAAACCTAGCCATCTATGTGATTTACATTCACATTAGATGCACGATTCCACTGTTGTTCCATCCTAGGTTCCTTGATATGCATATGAATCGTAATCACCTTTTCCTTTTCCTTAATTTTCTTTGATCAGACTTCAGACTGCAAGGAGGTTGATGTCGATTTCTCATTGGAGGATCTAGAGACTGTGAAAGTCATCGGGAAAGGAAGTGGAGGTGTAGTACAACTTGTTCAACACAAATGGGTTGGAAAACTTTTTGCCTTAAAGGTTGTTATATTGCTGGAACAAGGCTACACTATCGCATTTGTTTTTGGTCATTATACCTTTGTTTTCAAGTTACTTGCACATTTTGTTCCGTCATTTTATTATGGCTTTATATTAGTTGAAGAATCTGCTGCTGCTGTTGCTGCTGTAAGCTAGCAGTTCTTGATCTTTACATTTAAATGTGGAGATTGCTAGTAAACTACTATTACATTCATTTAGCTTTGAGAATAATTTCAATTTTCCTTTTCCAAAACCAGTAGGGGTTGGTCTCGGTTTACAAAATATTATTGAAGTCGAGAGTTCTTAATGAAATGTATGGCAGTGAACTAGTTTTATCCTTTTGATATATGAAATATAATAACTCAACGAGCTTTGCTAATAAAGTGAAGGCAAGATCTGGCAATGCCTCAGTGGAGACTGGCCATAGGAATATCTATTCATTGTTATGATTATTTGTATAATATGATAGGATTATGCTTGGTGTTGATTGATGGTTTTATTTGTTTCATGTCTTCTTTAAGCTAAAAATAAACTACCACCGTCAAAATAGTGGCACAATTAACTTGTCATCCTGTTTGTAAGTTTTTCAAATTCTGAAACCATGCTAACTTTTTTATTTTGGTCATCTTAGGTCTATTTTGGTAAAAAATAGTGGCTTGTTTATCTAAACTCCATAGTCTTGAGGATTTCAGTCTGTTTAGGCCTCCATAGTCTTGAGGATTTCAGTCTGTTTAGGCCTCCATACTTGGGCTAGTTAGGCTTTTGTACAAATTATATAATTCCGCTGAAAAATCTTTTCTGCCTCATGCTTCAGGTCATCCAAATGAACATACAAGAGGATATCCGTAAACAGATAGTGCAAGAACTGAAAATAAACCAAGCAGCACAATGTCCACATATTGTGGTCTGCCACCATTCTTTCTACCACAATGGTGCCATTTCTCTTGTGCTAGAATACATGGATCGTGGTTCTTTAGCTGATGTTGTCAGACAAGTCAAGACAATTCTTGAACCATATCTTGCAGTTGTTTGCAAACAGGTCTCAGGATTCTGTCAATAAAAAACTAAATGAAAAGGTGAAGTTTTCCCTCATGCTCACTCACTTCATAATTCCAGGTTTTACAAGGTCTTGTTTACTTGCATCATGAGAGACATGTTATACACAGAGACATCAAGCCATCCAATCTACTTGTGAACCATAAAGGGGATGTCAAAATCACTGATTTTGGAGTAAGTGCAATGCTTGCAAGTTCTATGGGTCAGAGAGATACATTTGTTGGGACTTACAATTACATGTCGGTATGTGAAATCAAAATCACTGATTGATTTTATGCAGATTGTTTATATTTGATATGAAGCATGAAATTCTTGTATTTTCTTCCCTTTCAGCCTGAGCGAATTAGCGGGGGAACATATGACTACAGCAGTGATATATGGAGCTTGGGTCTGGTAGTACTTGAATGTGCCATTGGAAGGTTTCCTTATTTGCAGTCTGAAGAGCAGCAAAGTTGGCCAAGCTTTTATGAACTTTTAGAGGCCATTGTAGCAAAGCCACCGCCTTCTGCTCCATCGGATCAATTTTCCCCCGAGTTCTGTTCATTCGTATCTGCTTGGTAATGATTTTTCTTTCTGCTTTTCCCATCTTGGTTAATTAAATAGCAATTCAAGTTTAGACATTTCATTATAATTACAATTGTGCAGCATCAAGAAGGACCCTAAAGAAAGATCATCTTCATTAGATCTTCTGGTTGGTAACCTTTGTTTTCTCCTTCAATTTATTATTGGCAATCGTACTCTCCTTAAACAATTCTCTTTGTGCTTCTAGAATCACCCTTTCATCAAGAAATTCGAGGACAAAGACATCGATGTTGGGATTCTCGTTGGCAGCTTGGATCCTCCTGTAAGTTTTCCTAGATAATAATCTTTAAACATTTACTTTTACTGTAATATGTTGGAA

mRNA sequence

CGCTCCTCATAGTTTGAATTTAATTCATTCATTTTTTCGCTGCTTTGACGAAGCAGATCGTCGCCTTCTTCACAGAGAAAAGGGGAAGAACCTCAATCGCACGTTTTTCCTCTCATTCTCTTCTTCCAATCTCCTCTATCTCTATTGAAACCCTAGGAATTTGATAAAGACAACATAAAAGATGAAGACCAAGACGCCATTGAAGCAGCTCAAGCTCTCTGTTCCCGCTCAAGAAACTTCTATCCGTTCTTTTCTAACTGCGAGCGGAACATTTCACGACGGCAATCTGCTTTTGAACCAGAAAGGATTGCGGCTTGTATCTGAAGAGAAAGAAACGCAGACTTCAGACTGCAAGGAGGTTGATGTCGATTTCTCATTGGAGGATCTAGAGACTGTGAAAGTCATCGGGAAAGGAAGTGGAGGTGTAGTACAACTTGTTCAACACAAATGGGTTGGAAAACTTTTTGCCTTAAAGGTTGTTATATTGCTGGAACAAGGCTACACTATCGCATTTGTTTTTGGTCATTATACCTTTGTTTTCAAGTTACTTGCACATTTTGTTCCGTCATTTTATTATGGCTTTATATTAGTTGAAGAATCTGCTGCTGCTGTTGCTGCTTTAGGCTTTTGTACAAATTATATAATTCCGCTGAAAAATCTTTTCTGCCTCATGCTTCAGGTCATCCAAATGAACATACAAGAGGATATCCGTAAACAGATAGTGCAAGAACTGAAAATAAACCAAGCAGCACAATGTCCACATATTGTGGTCTGCCACCATTCTTTCTACCACAATGGTGCCATTTCTCTTGTGCTAGAATACATGGATCGTGGTTCTTTAGCTGATGTTGTCAGACAAGTCAAGACAATTCTTGAACCATATCTTGCAGTTGTTTGCAAACAGGTTTTACAAGGTCTTGTTTACTTGCATCATGAGAGACATGTTATACACAGAGACATCAAGCCATCCAATCTACTTGTGAACCATAAAGGGGATGTCAAAATCACTGATTTTGGAGTAAGTGCAATGCTTGCAAGTTCTATGGGTCAGAGAGATACATTTGTTGGGACTTACAATTACATGTCGCCTGAGCGAATTAGCGGGGGAACATATGACTACAGCAGTGATATATGGAGCTTGGGTCTGGTAGTACTTGAATGTGCCATTGGAAGGTTTCCTTATTTGCAGTCTGAAGAGCAGCAAAGTTGGCCAAGCTTTTATGAACTTTTAGAGGCCATTGTAGCAAAGCCACCGCCTTCTGCTCCATCGGATCAATTTTCCCCCGAGTTCTGTTCATTCGTATCTGCTTGCATCAAGAAGGACCCTAAAGAAAGATCATCTTCATTAGATCTTCTGAATCACCCTTTCATCAAGAAATTCGAGGACAAAGACATCGATGTTGGGATTCTCGTTGGCAGCTTGGATCCTCCTGTAAGTTTTCCTAGATAATAATCTTTAAACATTTACTTTTACTGTAATATGTTGGAA

Coding sequence (CDS)

ATGAAGACCAAGACGCCATTGAAGCAGCTCAAGCTCTCTGTTCCCGCTCAAGAAACTTCTATCCGTTCTTTTCTAACTGCGAGCGGAACATTTCACGACGGCAATCTGCTTTTGAACCAGAAAGGATTGCGGCTTGTATCTGAAGAGAAAGAAACGCAGACTTCAGACTGCAAGGAGGTTGATGTCGATTTCTCATTGGAGGATCTAGAGACTGTGAAAGTCATCGGGAAAGGAAGTGGAGGTGTAGTACAACTTGTTCAACACAAATGGGTTGGAAAACTTTTTGCCTTAAAGGTTGTTATATTGCTGGAACAAGGCTACACTATCGCATTTGTTTTTGGTCATTATACCTTTGTTTTCAAGTTACTTGCACATTTTGTTCCGTCATTTTATTATGGCTTTATATTAGTTGAAGAATCTGCTGCTGCTGTTGCTGCTTTAGGCTTTTGTACAAATTATATAATTCCGCTGAAAAATCTTTTCTGCCTCATGCTTCAGGTCATCCAAATGAACATACAAGAGGATATCCGTAAACAGATAGTGCAAGAACTGAAAATAAACCAAGCAGCACAATGTCCACATATTGTGGTCTGCCACCATTCTTTCTACCACAATGGTGCCATTTCTCTTGTGCTAGAATACATGGATCGTGGTTCTTTAGCTGATGTTGTCAGACAAGTCAAGACAATTCTTGAACCATATCTTGCAGTTGTTTGCAAACAGGTTTTACAAGGTCTTGTTTACTTGCATCATGAGAGACATGTTATACACAGAGACATCAAGCCATCCAATCTACTTGTGAACCATAAAGGGGATGTCAAAATCACTGATTTTGGAGTAAGTGCAATGCTTGCAAGTTCTATGGGTCAGAGAGATACATTTGTTGGGACTTACAATTACATGTCGCCTGAGCGAATTAGCGGGGGAACATATGACTACAGCAGTGATATATGGAGCTTGGGTCTGGTAGTACTTGAATGTGCCATTGGAAGGTTTCCTTATTTGCAGTCTGAAGAGCAGCAAAGTTGGCCAAGCTTTTATGAACTTTTAGAGGCCATTGTAGCAAAGCCACCGCCTTCTGCTCCATCGGATCAATTTTCCCCCGAGTTCTGTTCATTCGTATCTGCTTGCATCAAGAAGGACCCTAAAGAAAGATCATCTTCATTAGATCTTCTGAATCACCCTTTCATCAAGAAATTCGAGGACAAAGACATCGATGTTGGGATTCTCGTTGGCAGCTTGGATCCTCCTGTAAGTTTTCCTAGATAA

Protein sequence

MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEVDVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVFKLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQIVQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCKQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPSAPSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSFPR
Homology
BLAST of Carg18601 vs. NCBI nr
Match: KAG7021309.1 (Mitogen-activated protein kinase kinase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 834.7 bits (2155), Expect = 3.5e-238
Identity = 422/422 (100.00%), Postives = 422/422 (100.00%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60
           MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV
Sbjct: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120
           DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF
Sbjct: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120

Query: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180
           KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI
Sbjct: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180

Query: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240
           VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK
Sbjct: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240

Query: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300
           QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY
Sbjct: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300

Query: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 360
           MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS
Sbjct: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 360

Query: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF 420
           APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF
Sbjct: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF 420

Query: 421 PR 423
           PR
Sbjct: 421 PR 422

BLAST of Carg18601 vs. NCBI nr
Match: XP_022937490.1 (mitogen-activated protein kinase kinase 6-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 676.4 bits (1744), Expect = 1.6e-190
Identity = 354/422 (83.89%), Postives = 354/422 (83.89%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60
           MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV
Sbjct: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120
           DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK                      
Sbjct: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK---------------------- 120

Query: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180
                                                         VIQMNIQEDIRKQI
Sbjct: 121 ----------------------------------------------VIQMNIQEDIRKQI 180

Query: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240
           VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK
Sbjct: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240

Query: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300
           QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY
Sbjct: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300

Query: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 360
           MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS
Sbjct: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 354

Query: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF 420
           APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF
Sbjct: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF 354

Query: 421 PR 423
           PR
Sbjct: 421 PR 354

BLAST of Carg18601 vs. NCBI nr
Match: XP_023537021.1 (mitogen-activated protein kinase kinase 6-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 673.3 bits (1736), Expect = 1.3e-189
Identity = 352/422 (83.41%), Postives = 353/422 (83.65%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60
           MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKE QTSDCKEV
Sbjct: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKEAQTSDCKEV 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120
           DVDFSL+DLETVKVIGKGSGGVVQLVQHKWVGKLFALK                      
Sbjct: 61  DVDFSLDDLETVKVIGKGSGGVVQLVQHKWVGKLFALK---------------------- 120

Query: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180
                                                         VIQMNIQEDIRKQI
Sbjct: 121 ----------------------------------------------VIQMNIQEDIRKQI 180

Query: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240
           VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK
Sbjct: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240

Query: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300
           QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY
Sbjct: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300

Query: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 360
           MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS
Sbjct: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 354

Query: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF 420
           APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF
Sbjct: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF 354

Query: 421 PR 423
           PR
Sbjct: 421 PR 354

BLAST of Carg18601 vs. NCBI nr
Match: XP_022965812.1 (mitogen-activated protein kinase kinase 6-like [Cucurbita maxima])

HSP 1 Score: 671.8 bits (1732), Expect = 3.9e-189
Identity = 352/422 (83.41%), Postives = 352/422 (83.41%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60
           MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV
Sbjct: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120
           D DFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK                      
Sbjct: 61  DADFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK---------------------- 120

Query: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180
                                                         VIQMNIQEDIRKQI
Sbjct: 121 ----------------------------------------------VIQMNIQEDIRKQI 180

Query: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240
           VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK
Sbjct: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240

Query: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300
           QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY
Sbjct: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300

Query: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 360
           MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS
Sbjct: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 354

Query: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF 420
           APSDQFSPEFCSFVSACIKKDPKERSSSL LLNHPFIKKFEDKDIDVGILVGSLDPPVSF
Sbjct: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLALLNHPFIKKFEDKDIDVGILVGSLDPPVSF 354

Query: 421 PR 423
           PR
Sbjct: 421 PR 354

BLAST of Carg18601 vs. NCBI nr
Match: XP_022937491.1 (mitogen-activated protein kinase kinase 6-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 665.6 bits (1716), Expect = 2.8e-187
Identity = 349/417 (83.69%), Postives = 349/417 (83.69%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60
           MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV
Sbjct: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120
           DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK                      
Sbjct: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK---------------------- 120

Query: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180
                                                         VIQMNIQEDIRKQI
Sbjct: 121 ----------------------------------------------VIQMNIQEDIRKQI 180

Query: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240
           VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK
Sbjct: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240

Query: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300
           QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY
Sbjct: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300

Query: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 360
           MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS
Sbjct: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 349

Query: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPP 418
           APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPP
Sbjct: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPP 349

BLAST of Carg18601 vs. ExPASy Swiss-Prot
Match: Q9FJV0 (Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK6 PE=1 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 1.4e-160
Identity = 296/423 (69.98%), Postives = 330/423 (78.01%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60
           +K K+ LKQLKLSVPAQE+ I SFLTASGTFHDG+ LLNQKGLRL S+EK+++ SD KE+
Sbjct: 2   VKIKSNLKQLKLSVPAQESPISSFLTASGTFHDGDFLLNQKGLRLTSDEKQSRQSDSKEL 61

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120
           D + + EDLETVKVIGKGSGGVVQLV+HKWVGK FA+K                      
Sbjct: 62  DFEITAEDLETVKVIGKGSGGVVQLVRHKWVGKFFAMK---------------------- 121

Query: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180
                                                         VIQMNIQE+IRKQI
Sbjct: 122 ----------------------------------------------VIQMNIQEEIRKQI 181

Query: 181 VQELKINQA-AQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVC 240
           VQELKINQA +QCPH+VVC+HSFYHNGA SLVLEYMDRGSLADV+RQVKTILEPYLAVVC
Sbjct: 182 VQELKINQASSQCPHVVVCYHSFYHNGAFSLVLEYMDRGSLADVIRQVKTILEPYLAVVC 241

Query: 241 KQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYN 300
           KQVL GLVYLH+ERHVIHRDIKPSNLLVNHKG+VKI+DFGVSA LASSMGQRDTFVGTYN
Sbjct: 242 KQVLLGLVYLHNERHVIHRDIKPSNLLVNHKGEVKISDFGVSASLASSMGQRDTFVGTYN 301

Query: 301 YMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPP 360
           YMSPERISG TYDYSSDIWSLG+ VLECAIGRFPYL+SE+QQ+ PSFYELL AIV  PPP
Sbjct: 302 YMSPERISGSTYDYSSDIWSLGMSVLECAIGRFPYLESEDQQNPPSFYELLAAIVENPPP 356

Query: 361 SAPSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVS 420
           +APSDQFSPEFCSFVSACI+KDP  R+SSLDLL+HPFIKKFEDKDID+GILVG+L+PPV+
Sbjct: 362 TAPSDQFSPEFCSFVSACIQKDPPARASSLDLLSHPFIKKFEDKDIDLGILVGTLEPPVN 356

Query: 421 FPR 423
           + R
Sbjct: 422 YLR 356

BLAST of Carg18601 vs. ExPASy Swiss-Prot
Match: Q5QN75 (Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MKK1 PE=1 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 1.5e-143
Identity = 267/421 (63.42%), Postives = 311/421 (73.87%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEE--KETQTSDCK 60
           M+ K P K+LKLSVPAQET +  FLTASGTF DG L LNQ+GL+L+SEE   E Q+++ K
Sbjct: 1   MRGKKPHKELKLSVPAQETPVDKFLTASGTFKDGELRLNQRGLQLISEETADEPQSTNLK 60

Query: 61  EVDVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTF 120
             DV  S++DLE ++VIGKGSGG+VQLV+HKWVG L+ALK                    
Sbjct: 61  VEDVQLSMDDLEMIQVIGKGSGGIVQLVRHKWVGTLYALK-------------------- 120

Query: 121 VFKLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRK 180
                                                            IQMNIQE +RK
Sbjct: 121 ------------------------------------------------GIQMNIQEAVRK 180

Query: 181 QIVQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVV 240
           QIVQELKINQA Q  HIV+CH SFYHNG I LVLEYMDRGSLAD+++QVKTILEPYLAV+
Sbjct: 181 QIVQELKINQATQNAHIVLCHQSFYHNGVIYLVLEYMDRGSLADIIKQVKTILEPYLAVL 240

Query: 241 CKQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTY 300
           CKQVL+GL+YLHHERHVIHRDIKPSNLLVN KG+VKITDFGVSA+LASSMGQRDTFVGTY
Sbjct: 241 CKQVLEGLLYLHHERHVIHRDIKPSNLLVNRKGEVKITDFGVSAVLASSMGQRDTFVGTY 300

Query: 301 NYMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPP 360
           NYM+PERISG +YDY SDIWSLGLV+LECAIGRFPY+ S E + W SFYELLEAIV +PP
Sbjct: 301 NYMAPERISGSSYDYKSDIWSLGLVILECAIGRFPYIPS-EGEGWLSFYELLEAIVDQPP 352

Query: 361 PSAPSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPV 420
           PSAP+DQFSPEFC+F+S+CI+KDP ER S+ +LLNHPFIKKFEDKD+D+ ILV SL+PP+
Sbjct: 361 PSAPADQFSPEFCAFISSCIQKDPAERMSASELLNHPFIKKFEDKDLDLRILVESLEPPM 352

BLAST of Carg18601 vs. ExPASy Swiss-Prot
Match: A9RWC9 (Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MKK1a PE=2 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 6.9e-112
Identity = 211/409 (51.59%), Postives = 278/409 (67.97%), Query Frame = 0

Query: 10  LKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEVDVDFSLEDL 69
           L+++VP +E +I  ++TASGTF DG+++LN++G+R+VS   +TQTS     + + SL DL
Sbjct: 15  LRVNVP-KENAISDYVTASGTFQDGDIMLNKEGIRVVS---QTQTS-TPAANGNISLSDL 74

Query: 70  ETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVFKLLAHFVPS 129
           ETVKV+GKGSGGVVQLV+HKW  + +ALK                               
Sbjct: 75  ETVKVLGKGSGGVVQLVRHKWTNETYALK------------------------------- 134

Query: 130 FYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQIVQELKINQA 189
                                                VI MNI+E  RKQIVQELKIN A
Sbjct: 135 -------------------------------------VIHMNIEETTRKQIVQELKINHA 194

Query: 190 AQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQV--KTILEPYLAVVCKQVLQGLV 249
           +QCP++V+C+H+FY+NG IS+VLEYMD GSLAD+++++  K I EP L VVCKQVL GL+
Sbjct: 195 SQCPYVVICYHAFYNNGLISIVLEYMDGGSLADIMKELEPKCIKEPNLGVVCKQVLLGLM 254

Query: 250 YLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNYMSPERIS 309
           YLH  RH+IHRDIKPSNLLVNHKG+VKI+DFGVSA+LA+SM  RDTFVGT  YMSPER+ 
Sbjct: 255 YLHQTRHIIHRDIKPSNLLVNHKGEVKISDFGVSAVLANSMAVRDTFVGTCTYMSPERVL 314

Query: 310 GGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPSAPSDQFS 369
           GGTY + SDIWSLGL +LECA+G++PY     ++ W +FYELL+ IV +PPP AP+DQFS
Sbjct: 315 GGTYGFDSDIWSLGLTLLECALGKYPYQPPGSEEGWMNFYELLQTIVDQPPPVAPADQFS 350

Query: 370 PEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDP 417
           PEFCSF+SAC++KDPK R ++ +LLNHPF+ K+++++  +  L+  L P
Sbjct: 375 PEFCSFISACLQKDPKCRPTAAELLNHPFVNKYDEEEYGLAKLLPPLVP 350

BLAST of Carg18601 vs. ExPASy Swiss-Prot
Match: A9SR33 (Mitogen-activated protein kinase kinase 1b OS=Physcomitrium patens OX=3218 GN=MKK1b PE=2 SV=2)

HSP 1 Score: 390.6 bits (1002), Expect = 2.3e-107
Identity = 202/397 (50.88%), Postives = 257/397 (64.74%), Query Frame = 0

Query: 10  LKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEVDVDFSLEDL 69
           L+++VP QE SI  FLTA+G FHD ++ L+  GLR+VS E    T+     D   SL DL
Sbjct: 10  LRVAVPKQENSIHRFLTANGVFHDDDIQLDHMGLRVVSSE---STAYANPPDAQLSLADL 69

Query: 70  ETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVFKLLAHFVPS 129
           E V+V+GKG+GG VQLV+HKW   ++ALK                               
Sbjct: 70  EAVRVLGKGAGGSVQLVRHKWTNDIYALK------------------------------- 129

Query: 130 FYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQIVQELKINQA 189
                                                 IQMNI E +RKQIVQELKINQ 
Sbjct: 130 -------------------------------------GIQMNINETVRKQIVQELKINQL 189

Query: 190 A--QCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCKQVLQGLV 249
              QCP+IV C+HSFYHNG IS+VLEYMDRGSLAD+++Q K I EPYLAV+  QVL+GL 
Sbjct: 190 TLHQCPYIVKCYHSFYHNGIISIVLEYMDRGSLADIIKQTKQIPEPYLAVISNQVLKGLN 249

Query: 250 YLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNYMSPERIS 309
           YLH  RH+IHRDIKPSNLL+N KG+VKI+DFGVSA+L SSM QRDTFVGTY YMSPER+ 
Sbjct: 250 YLHQVRHIIHRDIKPSNLLINQKGEVKISDFGVSAVLISSMAQRDTFVGTYTYMSPERLG 309

Query: 310 GGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPSAPSDQFS 369
           G +Y Y SDIWSLGL +LECA+G FPY    +++ W +F+ L+E ++ +PP +AP D+FS
Sbjct: 310 GQSYAYDSDIWSLGLTILECALGYFPYRPPGQEEGWNNFFMLMELVINQPPVAAPPDKFS 335

Query: 370 PEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKD 405
           PEFCSF++ACI+K P +R S+ DLL HPF++K+ +++
Sbjct: 370 PEFCSFIAACIQKRPGDRLSTADLLKHPFLQKYSEEE 335

BLAST of Carg18601 vs. ExPASy Swiss-Prot
Match: Q9S7U9 (Mitogen-activated protein kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MKK2 PE=1 SV=2)

HSP 1 Score: 383.3 bits (983), Expect = 3.6e-105
Identity = 199/399 (49.87%), Postives = 256/399 (64.16%), Query Frame = 0

Query: 10  LKLSVP-AQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEVDVDFSLED 69
           LKL++P A E SI  FLT SGTF DG+L +N+ G+R++S+ +    S  K  D   SL D
Sbjct: 10  LKLAIPVAGEQSITKFLTQSGTFKDGDLRVNKDGVRIISQLEPEVLSPIKPADDQLSLSD 69

Query: 70  LETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVFKLLAHFVP 129
           L+ VKVIGKGS GVVQLVQHKW G+ FALK                              
Sbjct: 70  LDMVKVIGKGSSGVVQLVQHKWTGQFFALK------------------------------ 129

Query: 130 SFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQIVQELKINQ 189
                                                 VIQ+NI E IRK I QELKINQ
Sbjct: 130 --------------------------------------VIQLNIDEAIRKAIAQELKINQ 189

Query: 190 AAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCKQVLQGLVY 249
           ++QCP++V  + SFY NGAISL+LEYMD GSLAD ++ VK I + YL+ + +QVLQGL+Y
Sbjct: 190 SSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSAIFRQVLQGLIY 249

Query: 250 LHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNYMSPERISG 309
           LHH+RH+IHRD+KPSNLL+NH+G+VKITDFGVS ++ ++ G  +TFVGTYNYMSPERI G
Sbjct: 250 LHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYNYMSPERIVG 309

Query: 310 GTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPSAPSDQFSP 369
             Y   SDIWSLGLVVLECA G+FPY    ++++W S +EL+EAIV +PPP+ PS  FSP
Sbjct: 310 NKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQPPPALPSGNFSP 340

Query: 370 EFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDV 408
           E  SF+S C++KDP  RSS+ +L+ HPF+ K++   I++
Sbjct: 370 ELSSFISTCLQKDPNSRSSAKELMEHPFLNKYDYSGINL 340

BLAST of Carg18601 vs. ExPASy TrEMBL
Match: A0A6J1FGT3 (mitogen-activated protein kinase kinase 6-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443886 PE=3 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 7.7e-191
Identity = 354/422 (83.89%), Postives = 354/422 (83.89%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60
           MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV
Sbjct: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120
           DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK                      
Sbjct: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK---------------------- 120

Query: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180
                                                         VIQMNIQEDIRKQI
Sbjct: 121 ----------------------------------------------VIQMNIQEDIRKQI 180

Query: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240
           VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK
Sbjct: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240

Query: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300
           QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY
Sbjct: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300

Query: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 360
           MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS
Sbjct: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 354

Query: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF 420
           APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF
Sbjct: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF 354

Query: 421 PR 423
           PR
Sbjct: 421 PR 354

BLAST of Carg18601 vs. ExPASy TrEMBL
Match: A0A6J1HLB3 (mitogen-activated protein kinase kinase 6-like OS=Cucurbita maxima OX=3661 GN=LOC111465588 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.9e-189
Identity = 352/422 (83.41%), Postives = 352/422 (83.41%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60
           MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV
Sbjct: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120
           D DFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK                      
Sbjct: 61  DADFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK---------------------- 120

Query: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180
                                                         VIQMNIQEDIRKQI
Sbjct: 121 ----------------------------------------------VIQMNIQEDIRKQI 180

Query: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240
           VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK
Sbjct: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240

Query: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300
           QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY
Sbjct: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300

Query: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 360
           MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS
Sbjct: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 354

Query: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF 420
           APSDQFSPEFCSFVSACIKKDPKERSSSL LLNHPFIKKFEDKDIDVGILVGSLDPPVSF
Sbjct: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLALLNHPFIKKFEDKDIDVGILVGSLDPPVSF 354

Query: 421 PR 423
           PR
Sbjct: 421 PR 354

BLAST of Carg18601 vs. ExPASy TrEMBL
Match: A0A6J1FAH3 (mitogen-activated protein kinase kinase 6-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443886 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.4e-187
Identity = 349/417 (83.69%), Postives = 349/417 (83.69%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60
           MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV
Sbjct: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120
           DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK                      
Sbjct: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK---------------------- 120

Query: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180
                                                         VIQMNIQEDIRKQI
Sbjct: 121 ----------------------------------------------VIQMNIQEDIRKQI 180

Query: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240
           VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK
Sbjct: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240

Query: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300
           QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY
Sbjct: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300

Query: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 360
           MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS
Sbjct: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 349

Query: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPP 418
           APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPP
Sbjct: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPP 349

BLAST of Carg18601 vs. ExPASy TrEMBL
Match: A0A0A0LEX8 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000780 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 7.0e-184
Identity = 341/422 (80.81%), Postives = 349/422 (82.70%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60
           MKTKTPLKQLKLSVP QETSIRSFLTASGTFHDGNLLLNQKG+RL+SEEKE+QT+D KE+
Sbjct: 1   MKTKTPLKQLKLSVPVQETSIRSFLTASGTFHDGNLLLNQKGMRLISEEKESQTTDSKEL 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120
           DVDFSLEDLETVKVIGKGSGGVVQLV+HKWVGKLFALK                      
Sbjct: 61  DVDFSLEDLETVKVIGKGSGGVVQLVRHKWVGKLFALK---------------------- 120

Query: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180
                                                         VIQMNIQEDIRKQI
Sbjct: 121 ----------------------------------------------VIQMNIQEDIRKQI 180

Query: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240
           VQELKINQAAQC HIVVC+HSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK
Sbjct: 181 VQELKINQAAQCSHIVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240

Query: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300
           QVLQGLVYLHHERHVIHRDIKPSNLLVNHKG+VKITDFGVSAMLASSMGQRDTFVGTYNY
Sbjct: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGEVKITDFGVSAMLASSMGQRDTFVGTYNY 300

Query: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 360
           MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS
Sbjct: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 354

Query: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF 420
           AP DQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILV SLDPPVSF
Sbjct: 361 APPDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVASLDPPVSF 354

Query: 421 PR 423
           PR
Sbjct: 421 PR 354

BLAST of Carg18601 vs. ExPASy TrEMBL
Match: A0A6J1DPB3 (mitogen-activated protein kinase kinase 6 OS=Momordica charantia OX=3673 GN=LOC111021852 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 7.0e-184
Identity = 340/422 (80.57%), Postives = 348/422 (82.46%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60
           MKTKTPLKQLKLSVPAQE +I SFLTASGTFHDGNLLLNQKGLRL+SEEKE QTSD KE+
Sbjct: 1   MKTKTPLKQLKLSVPAQEANIHSFLTASGTFHDGNLLLNQKGLRLISEEKEAQTSDSKEL 60

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120
           D DFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK                      
Sbjct: 61  DADFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALK---------------------- 120

Query: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180
                                                         VIQMNIQEDIRKQI
Sbjct: 121 ----------------------------------------------VIQMNIQEDIRKQI 180

Query: 181 VQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240
           VQELKINQAAQCPH+VVC+HSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK
Sbjct: 181 VQELKINQAAQCPHVVVCYHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCK 240

Query: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNY 300
           QVLQGLVYLHHERHVIHRDIKPSNLLVNHKG+VKITDFGVSAMLASSMGQRDTFVGTYNY
Sbjct: 241 QVLQGLVYLHHERHVIHRDIKPSNLLVNHKGEVKITDFGVSAMLASSMGQRDTFVGTYNY 300

Query: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 360
           MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS
Sbjct: 301 MSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPS 354

Query: 361 APSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVSF 420
           AP DQFSPEFCSFVSACIKKDPKERSSSLDLL+HPFIKKFEDKDID+GILVGSLDPPVSF
Sbjct: 361 APPDQFSPEFCSFVSACIKKDPKERSSSLDLLDHPFIKKFEDKDIDLGILVGSLDPPVSF 354

Query: 421 PR 423
           PR
Sbjct: 421 PR 354

BLAST of Carg18601 vs. TAIR 10
Match: AT5G56580.1 (MAP kinase kinase 6 )

HSP 1 Score: 567.4 bits (1461), Expect = 9.7e-162
Identity = 296/423 (69.98%), Postives = 330/423 (78.01%), Query Frame = 0

Query: 1   MKTKTPLKQLKLSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEV 60
           +K K+ LKQLKLSVPAQE+ I SFLTASGTFHDG+ LLNQKGLRL S+EK+++ SD KE+
Sbjct: 2   VKIKSNLKQLKLSVPAQESPISSFLTASGTFHDGDFLLNQKGLRLTSDEKQSRQSDSKEL 61

Query: 61  DVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVF 120
           D + + EDLETVKVIGKGSGGVVQLV+HKWVGK FA+K                      
Sbjct: 62  DFEITAEDLETVKVIGKGSGGVVQLVRHKWVGKFFAMK---------------------- 121

Query: 121 KLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQI 180
                                                         VIQMNIQE+IRKQI
Sbjct: 122 ----------------------------------------------VIQMNIQEEIRKQI 181

Query: 181 VQELKINQA-AQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVC 240
           VQELKINQA +QCPH+VVC+HSFYHNGA SLVLEYMDRGSLADV+RQVKTILEPYLAVVC
Sbjct: 182 VQELKINQASSQCPHVVVCYHSFYHNGAFSLVLEYMDRGSLADVIRQVKTILEPYLAVVC 241

Query: 241 KQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYN 300
           KQVL GLVYLH+ERHVIHRDIKPSNLLVNHKG+VKI+DFGVSA LASSMGQRDTFVGTYN
Sbjct: 242 KQVLLGLVYLHNERHVIHRDIKPSNLLVNHKGEVKISDFGVSASLASSMGQRDTFVGTYN 301

Query: 301 YMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPP 360
           YMSPERISG TYDYSSDIWSLG+ VLECAIGRFPYL+SE+QQ+ PSFYELL AIV  PPP
Sbjct: 302 YMSPERISGSTYDYSSDIWSLGMSVLECAIGRFPYLESEDQQNPPSFYELLAAIVENPPP 356

Query: 361 SAPSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGILVGSLDPPVS 420
           +APSDQFSPEFCSFVSACI+KDP  R+SSLDLL+HPFIKKFEDKDID+GILVG+L+PPV+
Sbjct: 362 TAPSDQFSPEFCSFVSACIQKDPPARASSLDLLSHPFIKKFEDKDIDLGILVGTLEPPVN 356

Query: 421 FPR 423
           + R
Sbjct: 422 YLR 356

BLAST of Carg18601 vs. TAIR 10
Match: AT4G29810.1 (MAP kinase kinase 2 )

HSP 1 Score: 383.3 bits (983), Expect = 2.6e-106
Identity = 199/399 (49.87%), Postives = 256/399 (64.16%), Query Frame = 0

Query: 10  LKLSVP-AQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEVDVDFSLED 69
           LKL++P A E SI  FLT SGTF DG+L +N+ G+R++S+ +    S  K  D   SL D
Sbjct: 10  LKLAIPVAGEQSITKFLTQSGTFKDGDLRVNKDGVRIISQLEPEVLSPIKPADDQLSLSD 69

Query: 70  LETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVFKLLAHFVP 129
           L+ VKVIGKGS GVVQLVQHKW G+ FALK                              
Sbjct: 70  LDMVKVIGKGSSGVVQLVQHKWTGQFFALK------------------------------ 129

Query: 130 SFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQIVQELKINQ 189
                                                 VIQ+NI E IRK I QELKINQ
Sbjct: 130 --------------------------------------VIQLNIDEAIRKAIAQELKINQ 189

Query: 190 AAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCKQVLQGLVY 249
           ++QCP++V  + SFY NGAISL+LEYMD GSLAD ++ VK I + YL+ + +QVLQGL+Y
Sbjct: 190 SSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSAIFRQVLQGLIY 249

Query: 250 LHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNYMSPERISG 309
           LHH+RH+IHRD+KPSNLL+NH+G+VKITDFGVS ++ ++ G  +TFVGTYNYMSPERI G
Sbjct: 250 LHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYNYMSPERIVG 309

Query: 310 GTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPSAPSDQFSP 369
             Y   SDIWSLGLVVLECA G+FPY    ++++W S +EL+EAIV +PPP+ PS  FSP
Sbjct: 310 NKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQPPPALPSGNFSP 340

Query: 370 EFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDV 408
           E  SF+S C++KDP  RSS+ +L+ HPF+ K++   I++
Sbjct: 370 ELSSFISTCLQKDPNSRSSAKELMEHPFLNKYDYSGINL 340

BLAST of Carg18601 vs. TAIR 10
Match: AT4G29810.2 (MAP kinase kinase 2 )

HSP 1 Score: 375.6 bits (963), Expect = 5.4e-104
Identity = 199/408 (48.77%), Postives = 256/408 (62.75%), Query Frame = 0

Query: 10  LKLSVP-AQETSIRSFL---------TASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKE 69
           LKL++P A E SI  FL         T SGTF DG+L +N+ G+R++S+ +    S  K 
Sbjct: 10  LKLAIPVAGEQSITKFLRKGFGSLCRTQSGTFKDGDLRVNKDGVRIISQLEPEVLSPIKP 69

Query: 70  VDVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFV 129
            D   SL DL+ VKVIGKGS GVVQLVQHKW G+ FALK                     
Sbjct: 70  ADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALK--------------------- 129

Query: 130 FKLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQ 189
                                                          VIQ+NI E IRK 
Sbjct: 130 -----------------------------------------------VIQLNIDEAIRKA 189

Query: 190 IVQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVC 249
           I QELKINQ++QCP++V  + SFY NGAISL+LEYMD GSLAD ++ VK I + YL+ + 
Sbjct: 190 IAQELKINQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSAIF 249

Query: 250 KQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYN 309
           +QVLQGL+YLHH+RH+IHRD+KPSNLL+NH+G+VKITDFGVS ++ ++ G  +TFVGTYN
Sbjct: 250 RQVLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYN 309

Query: 310 YMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPP 369
           YMSPERI G  Y   SDIWSLGLVVLECA G+FPY    ++++W S +EL+EAIV +PPP
Sbjct: 310 YMSPERIVGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQPPP 349

Query: 370 SAPSDQFSPEFCSFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDV 408
           + PS  FSPE  SF+S C++KDP  RSS+ +L+ HPF+ K++   I++
Sbjct: 370 ALPSGNFSPELSSFISTCLQKDPNSRSSAKELMEHPFLNKYDYSGINL 349

BLAST of Carg18601 vs. TAIR 10
Match: AT4G29810.3 (MAP kinase kinase 2 )

HSP 1 Score: 362.1 bits (928), Expect = 6.2e-100
Identity = 194/393 (49.36%), Postives = 246/393 (62.60%), Query Frame = 0

Query: 10  LKLSVP-AQETSIRSFL---------TASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKE 69
           LKL++P A E SI  FL         T SGTF DG+L +N+ G+R++S+ +    S  K 
Sbjct: 10  LKLAIPVAGEQSITKFLRKGFGSLCRTQSGTFKDGDLRVNKDGVRIISQLEPEVLSPIKP 69

Query: 70  VDVDFSLEDLETVKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFV 129
            D   SL DL+ VKVIGKGS GVVQLVQHKW G+ FALK                     
Sbjct: 70  ADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALK--------------------- 129

Query: 130 FKLLAHFVPSFYYGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQ 189
                                                          VIQ+NI E IRK 
Sbjct: 130 -----------------------------------------------VIQLNIDEAIRKA 189

Query: 190 IVQELKINQAAQCPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVC 249
           I QELKINQ++QCP++V  + SFY NGAISL+LEYMD GSLAD ++ VK I + YL+ + 
Sbjct: 190 IAQELKINQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSAIF 249

Query: 250 KQVLQGLVYLHHERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYN 309
           +QVLQGL+YLHH+RH+IHRD+KPSNLL+NH+G+VKITDFGVS ++ ++ G  +TFVGTYN
Sbjct: 250 RQVLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYN 309

Query: 310 YMSPERISGGTYDYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPP 369
           YMSPERI G  Y   SDIWSLGLVVLECA G+FPY    ++++W S +EL+EAIV +PPP
Sbjct: 310 YMSPERIVGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQPPP 334

Query: 370 SAPSDQFSPEFCSFVSACIKKDPKERSSSLDLL 393
           + PS  FSPE  SF+S C++KDP  RSS+ +L+
Sbjct: 370 ALPSGNFSPELSSFISTCLQKDPNSRSSAKELM 334

BLAST of Carg18601 vs. TAIR 10
Match: AT4G26070.2 (MAP kinase/ ERK kinase 1 )

HSP 1 Score: 360.5 bits (924), Expect = 1.8e-99
Identity = 188/411 (45.74%), Postives = 252/411 (61.31%), Query Frame = 0

Query: 12  LSVPAQETSIRSFLTASGTFHDGNLLLNQKGLRLVSEEKETQTSDCKEVDVDFSLEDLET 71
           + +P  E SI  FLT SGTF DG+L +N+ G++ VS  +       + +D   SL DLE 
Sbjct: 11  ICLPPLEQSISKFLTQSGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEV 70

Query: 72  VKVIGKGSGGVVQLVQHKWVGKLFALKVVILLEQGYTIAFVFGHYTFVFKLLAHFVPSFY 131
           +KVIGKGS G VQLV+HK   + FALK                                 
Sbjct: 71  IKVIGKGSSGNVQLVKHKLTQQFFALK--------------------------------- 130

Query: 132 YGFILVEESAAAVAALGFCTNYIIPLKNLFCLMLQVIQMNIQEDIRKQIVQELKINQAAQ 191
                                              VIQ+N +E   + I QEL+IN ++Q
Sbjct: 131 -----------------------------------VIQLNTEESTCRAISQELRINLSSQ 190

Query: 192 CPHIVVCHHSFYHNGAISLVLEYMDRGSLADVVRQVKTILEPYLAVVCKQVLQGLVYLHH 251
           CP++V C+ SFYHNG +S++LE+MD GSLAD++++V  + E  L+ +CK+VL+GL Y+HH
Sbjct: 191 CPYLVSCYQSFYHNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHH 250

Query: 252 ERHVIHRDIKPSNLLVNHKGDVKITDFGVSAMLASSMGQRDTFVGTYNYMSPERISGGTY 311
           ER +IHRD+KPSNLL+NH+G+VKITDFGVS +L S+    ++FVGTY YMSPERISG  Y
Sbjct: 251 ERRIIHRDLKPSNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLY 310

Query: 312 DYSSDIWSLGLVVLECAIGRFPYLQSEEQQSWPSFYELLEAIVAKPPPSAPSDQFSPEFC 371
              SDIWSLGLV+LECA G+FPY   E ++ W S YEL++AIV  PPP APS+ FSPEFC
Sbjct: 311 SNKSDIWSLGLVLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFC 353

Query: 372 SFVSACIKKDPKERSSSLDLLNHPFIKKFEDKDIDVGIL---VGSLDPPVS 420
           SF+S C++KDP++R S+ +LL H F+K FED D ++       GSL PP++
Sbjct: 371 SFISQCVQKDPRDRKSAKELLEHKFVKMFEDSDTNLSAYFTDAGSLIPPLA 353

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021309.13.5e-238100.00Mitogen-activated protein kinase kinase 6 [Cucurbita argyrosperma subsp. argyros... [more]
XP_022937490.11.6e-19083.89mitogen-activated protein kinase kinase 6-like isoform X1 [Cucurbita moschata][more]
XP_023537021.11.3e-18983.41mitogen-activated protein kinase kinase 6-like isoform X1 [Cucurbita pepo subsp.... [more]
XP_022965812.13.9e-18983.41mitogen-activated protein kinase kinase 6-like [Cucurbita maxima][more]
XP_022937491.12.8e-18783.69mitogen-activated protein kinase kinase 6-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FJV01.4e-16069.98Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q5QN751.5e-14363.42Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=399... [more]
A9RWC96.9e-11251.59Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MK... [more]
A9SR332.3e-10750.88Mitogen-activated protein kinase kinase 1b OS=Physcomitrium patens OX=3218 GN=MK... [more]
Q9S7U93.6e-10549.87Mitogen-activated protein kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Match NameE-valueIdentityDescription
A0A6J1FGT37.7e-19183.89mitogen-activated protein kinase kinase 6-like isoform X1 OS=Cucurbita moschata ... [more]
A0A6J1HLB31.9e-18983.41mitogen-activated protein kinase kinase 6-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1FAH31.4e-18783.69mitogen-activated protein kinase kinase 6-like isoform X2 OS=Cucurbita moschata ... [more]
A0A0A0LEX87.0e-18480.81Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000... [more]
A0A6J1DPB37.0e-18480.57mitogen-activated protein kinase kinase 6 OS=Momordica charantia OX=3673 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT5G56580.19.7e-16269.98MAP kinase kinase 6 [more]
AT4G29810.12.6e-10649.87MAP kinase kinase 2 [more]
AT4G29810.25.4e-10448.77MAP kinase kinase 2 [more]
AT4G29810.36.2e-10049.36MAP kinase kinase 2 [more]
AT4G26070.21.8e-9945.74MAP kinase/ ERK kinase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 69..397
e-value: 4.0E-74
score: 262.2
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 171..397
e-value: 1.0E-56
score: 192.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 69..397
score: 42.887516
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 158..216
e-value: 3.8E-13
score: 51.3
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 218..411
e-value: 3.9E-58
score: 198.9
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 11..101
e-value: 1.8E-9
score: 39.1
NoneNo IPR availablePIRSRPIRSR000606-51PIRSR000606-51coord: 178..388
e-value: 9.8E-30
score: 101.3
NoneNo IPR availablePANTHERPTHR24361:SF816MITOGEN-ACTIVATED PROTEIN KINASE KINASE 6coord: 164..421
NoneNo IPR availablePANTHERPTHR24361:SF816MITOGEN-ACTIVATED PROTEIN KINASE KINASE 6coord: 1..101
NoneNo IPR availablePANTHERPTHR24361MITOGEN-ACTIVATED KINASE KINASE KINASEcoord: 164..421
NoneNo IPR availablePANTHERPTHR24361MITOGEN-ACTIVATED KINASE KINASE KINASEcoord: 1..101
NoneNo IPR availableCDDcd06623PKc_MAPKK_plant_likecoord: 67..401
e-value: 1.53495E-139
score: 397.733
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 255..267
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 75..98
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 65..404

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg18601-RACarg18601-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002229 defense response to oomycetes
biological_process GO:0010311 lateral root formation
biological_process GO:0007112 male meiosis cytokinesis
biological_process GO:0000165 MAPK cascade
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0009524 phragmoplast
molecular_function GO:0005524 ATP binding
molecular_function GO:0004708 MAP kinase kinase activity
molecular_function GO:0051019 mitogen-activated protein kinase binding
molecular_function GO:0031435 mitogen-activated protein kinase kinase kinase binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity