Carg18456 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18456
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Mannan endo-1,4-beta-mannosidase
Location	Carg_Chr20: 947477 .. 950390 (-)
RNA-Seq Expression	Carg18456
Synteny	Carg18456

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGGGAATGGGCTCTTTTACCCGATTCTCGGACTCGCATGTTGTTTGGCTTTCATTTACATATCGTTTGGGGACTGGAAGCTTGTGGATACTAAAGCCCCCAAAATGGGTTTTGTAGAGAGGAATGGAACGCAGTTCATGGTGAATGGGAGAGCGTTTTATATCAATGGATGGAATTCTTACTGGTTTATGGACCATGCCGTTGAGGGATACAGAAAGCCCAGAGTCAGAGCGATGCTGCGGGCGGCGAGGAAGATGGGGCTTACAGTTTGCCGAACTTGGGCCTTCAGCGACGGTGGCTACAATGCCCTCCAGGTTTTTCCCGGCAGGTTTAACGAGCAAGTCTTTAAGGTAAGTGAAACGTTTGTTGCTGTGATGAGCTATATTTTTCTGAGAGATTCCTGTTGTTTCTGTGATTGGGTTCTTTACTTGTTGAGCCCTTGTTTGATTCCTACTCTCATGTGCCTTGGTTCTGTCTATGTGATTGGCTTCTTTCTCTGAAATTCTATGTCAATGTCTGTTTTAGAGAGCGATGAAGTGTGTCTGCAGGGGTTGGAAGGCTCCATTGGCCAAAAAATGGAAGCTGGGTGCTTCCACCGACAGAAGTGTCGTGTGAATATGATTTGAATTCTCACGTTCTATAAGCCGATTGAAAAACATTATCACTTATATTTCTATGAAAGCTCAAGGTGGTTGTGTTTGTTTACTTCATCATATAGTTCTAAGTTTATAAATCGAGGACTGGAGTTCAAGGACGGATCTCACGTCGGTTGGAGAGGGGAACGAAACATTTCTTATAAGGGTGTGGAAACCTCTCCCTAACACTTTTTAAAACCTCGAGGGAAAAAGCCCAGAAGGGAAAGACCAAAGAGGACAATATCTACTAGCGGTGTGCTTGGGCTGTTATAAATGGTATCAGAGTCAGACACTAGGTGGTGTGTCAACGAGGACGTTGGACCCTTAAGAAGGGTGGATGATGAGATCCCACATCGGTTGGAGAGGGGAACGAAGCATCCCTTCTTACAAGGGTGTGAAAATCCTCCTCCTAACAGATACGTTTTTAAACCTTAGGGAAAGCTGAAAGAAGACAATATTTGGTAGCAGTAATTACAATGTCTTTCGCTCTCTTGTTTCTAAAATGACACATTATTCAAACTACGAACAATAAAATATATGCATATATGTTTTCTATGGTGATTTGTTTGGTTTGACGATAAGTTCAATTTGCTCAAGTTGTCAGTTATCAGCTGAAGTTTTAACTGGTTTTTCCTGGTGGCAGGCTTTGGATCATGTCATTGCAGAATCAAGACAACATGGGATAAGGCTTCTCCTTAGCTTAGTCAACAACTTGCAAGCATACGGTGGGAAGACTCAGTATGTGAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTCTATGATCCCTCGATACGCATTTATTTTAAGAATTATATTAAGGTACGTTCCATATTTATCACTTAGTTTGTCCTGTATGGTTCGTAATGTTGATGATTTGGGTATTCATCCTGAATTTATGCGGTGGATTTTGAGTGTGAGATCCCACATCGGCTGGAGAAGTGAACGAAACATTCCTTGTAAGGGTGTGAAAACCTCTCCCAAACAGACGCATTTTAAAACTTTGAGGGAAAGCTCAGAAGGGAAAAACCTAAAGAGGATAATATCCGCTAATGATGGGCTTGAGCTGTTATAAATGTTATCATAGCCAGACACTGAGCGGTGTGCTAGCGAGGATGCTGTGCCCCCAAAGGGGGTGGATTGTGAGATCCCACATCGACTAGAGAGGGGAACAGAGCATTTCTTATAAGGGTGTAAAAACCTCTATCTAATAGACGTGTTTTAAACCTTGAGGGGAAGCCCAAAAAAAGAAGGTCTCACGAGAAGAATTTCTGCTAGCACTACATTATGGTGTCTTTGCTGTTCTTACATTGCTACCTTATGGTGTTTACTCGTTCTAGACTGTCCTAACAAGAAGGAACTCCATCACTGGAATTGAATATAGAAACGATCCGACCATCTTCGGATGGGAATTGATTAACGAGCCACGTTGTATGACCGATGCCTCAGGCGATACTCTTCAAGTAAGTTGATACTCCTCAATATAGTTGTAAGATTCTGATTCTTCTGGCTTCTGAATGTTAAACCAAATGAAAACTGAACTTGTAGGAGTGGATTGAAGAAATGTCAGCTTACATTAAATCGATTGACAAGAAGCATCTATTAACTGTTGGGTTAGAAGGATTTTATGGTCCAAATAGCCCCAAGAAATCTACTGTGAACCCGGAAGAGTGGGCATCCAGACTTGGCTCTGATTTCATCCGCAATTCTGAGATCCCACACATAGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTAAGCATTGAATGATAGTACTCAATGATTCAGCTCATGTCTATGCTCTTAAACCCGTTACTTATACGACGAAATTAACATTCCGTGTTGATTAGGTTCCATGATCAAGATTTTGAAGACGAACTGAAATTTGTATCCAAGTGGATGCTCTCTCATATTGAAGATGGTGACAAAGAGTTGAAGAAACCAGTCATGTTTACTGAATTTGGACTGTCAGACCTGAACAAAGGCTTCACCCCAGCTCAGCGGGATAGATTTTACAAAACTGTTTACGACGTCATTTACAAATCTGCCAAGAGGAAGCGATCGGGAGCTGGCTCTTTAGCCTGGCAGTTCCTTGTCGAAGGAATGGAAGAGAGTAACGACGATTTCGGGATAGTCCCGTGGGAAAGATCATCGATACATCGGCTTATTATAGAGCAATCATGCAGGTTGGCAAGGATTGGTGGAGACATCCAACAGTTAAAAGCATTGAAAAATGTGTGCTGAGAGATGG

mRNA sequence

ATGGCGGGGAATGGGCTCTTTTACCCGATTCTCGGACTCGCATGTTGTTTGGCTTTCATTTACATATCGTTTGGGGACTGGAAGCTTGTGGATACTAAAGCCCCCAAAATGGGTTTTGTAGAGAGGAATGGAACGCAGTTCATGGTGAATGGGAGAGCGTTTTATATCAATGGATGGAATTCTTACTGGTTTATGGACCATGCCGTTGAGGGATACAGAAAGCCCAGAGTCAGAGCGATGCTGCGGGCGGCGAGGAAGATGGGGCTTACAGTTTGCCGAACTTGGGCCTTCAGCGACGGTGGCTACAATGCCCTCCAGGTTTTTCCCGGCAGGTTTAACGAGCAAGTCTTTAAGGCTTTGGATCATGTCATTGCAGAATCAAGACAACATGGGATAAGGCTTCTCCTTAGCTTAGTCAACAACTTGCAAGCATACGGTGGGAAGACTCAGTATGTGAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTCTATGATCCCTCGATACGCATTTATTTTAAGAATTATATTAAGACTGTCCTAACAAGAAGGAACTCCATCACTGGAATTGAATATAGAAACGATCCGACCATCTTCGGATGGGAATTGATTAACGAGCCACGTTGTATGACCGATGCCTCAGGCGATACTCTTCAAGAGTGGATTGAAGAAATGTCAGCTTACATTAAATCGATTGACAAGAAGCATCTATTAACTGTTGGGTTAGAAGGATTTTATGGTCCAAATAGCCCCAAGAAATCTACTGTGAACCCGGAAGAGTGGGCATCCAGACTTGGCTCTGATTTCATCCGCAATTCTGAGATCCCACACATAGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTTCCATGATCAAGATTTTGAAGACGAACTGAAATTTGTATCCAAGTGGATGCTCTCTCATATTGAAGATGGTGACAAAGAGTTGAAGAAACCAGTCATGTTTACTGAATTTGGACTGTCAGACCTGAACAAAGGCTTCACCCCAGCTCAGCGGGATAGATTTTACAAAACTGTTTACGACGTCATTTACAAATCTGCCAAGAGGAAGCGATCGGGAGCTGGCTCTTTAGCCTGGCAGTTCCTTGTCGAAGGAATGGAAGAGAGTAACGACGATTTCGGGATAGTCCCGTGGGAAAGATCATCGATACATCGGCTTATTATAGAGCAATCATGCAGGTTGGCAAGGATTGGTGGAGACATCCAACAGTTAAAAGCATTGAAAAATGTGTGCTGAGAGATGG

Coding sequence (CDS)

ATGGCGGGGAATGGGCTCTTTTACCCGATTCTCGGACTCGCATGTTGTTTGGCTTTCATTTACATATCGTTTGGGGACTGGAAGCTTGTGGATACTAAAGCCCCCAAAATGGGTTTTGTAGAGAGGAATGGAACGCAGTTCATGGTGAATGGGAGAGCGTTTTATATCAATGGATGGAATTCTTACTGGTTTATGGACCATGCCGTTGAGGGATACAGAAAGCCCAGAGTCAGAGCGATGCTGCGGGCGGCGAGGAAGATGGGGCTTACAGTTTGCCGAACTTGGGCCTTCAGCGACGGTGGCTACAATGCCCTCCAGGTTTTTCCCGGCAGGTTTAACGAGCAAGTCTTTAAGGCTTTGGATCATGTCATTGCAGAATCAAGACAACATGGGATAAGGCTTCTCCTTAGCTTAGTCAACAACTTGCAAGCATACGGTGGGAAGACTCAGTATGTGAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTCTATGATCCCTCGATACGCATTTATTTTAAGAATTATATTAAGACTGTCCTAACAAGAAGGAACTCCATCACTGGAATTGAATATAGAAACGATCCGACCATCTTCGGATGGGAATTGATTAACGAGCCACGTTGTATGACCGATGCCTCAGGCGATACTCTTCAAGAGTGGATTGAAGAAATGTCAGCTTACATTAAATCGATTGACAAGAAGCATCTATTAACTGTTGGGTTAGAAGGATTTTATGGTCCAAATAGCCCCAAGAAATCTACTGTGAACCCGGAAGAGTGGGCATCCAGACTTGGCTCTGATTTCATCCGCAATTCTGAGATCCCACACATAGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTTCCATGATCAAGATTTTGAAGACGAACTGAAATTTGTATCCAAGTGGATGCTCTCTCATATTGAAGATGGTGACAAAGAGTTGAAGAAACCAGTCATGTTTACTGAATTTGGACTGTCAGACCTGAACAAAGGCTTCACCCCAGCTCAGCGGGATAGATTTTACAAAACTGTTTACGACGTCATTTACAAATCTGCCAAGAGGAAGCGATCGGGAGCTGGCTCTTTAGCCTGGCAGTTCCTTGTCGAAGGAATGGAAGAGAGTAACGACGATTTCGGGATAGTCCCGTGGGAAAGATCATCGATACATCGGCTTATTATAGAGCAATCATGCAGGTTGGCAAGGATTGGTGGAGACATCCAACAGTTAAAAGCATTGAAAAATGTGTGCTGA

Protein sequence

MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWNSYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQLKALKNVC

Homology

[Close]

BLAST of Carg18456 vs. NCBI nr
Match: KAG7010376.1 (Mannan endo-1,4-beta-mannosidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 896.7 bits (2316), Expect = 7.6e-257
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360
           DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK
Sbjct: 301 DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNVC 427
           ALKNVC
Sbjct: 421 ALKNVC 426

[Close]

BLAST of Carg18456 vs. NCBI nr
Match: XP_022944323.1 (mannan endo-1,4-beta-mannosidase 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 892.9 bits (2306), Expect = 1.1e-255
Identity = 424/426 (99.53%), Postives = 426/426 (100.00%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360
           DFE+ELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK
Sbjct: 301 DFEEELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSI+RLIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNVC 427
           ALKNVC
Sbjct: 421 ALKNVC 426

[Close]

BLAST of Carg18456 vs. NCBI nr
Match: KAG6570514.1 (Mannan endo-1,4-beta-mannosidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 892.9 bits (2306), Expect = 1.1e-255
Identity = 424/426 (99.53%), Postives = 426/426 (100.00%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360
           DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK
Sbjct: 301 DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSI++LIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNVC 427
           ALKNVC
Sbjct: 421 ALKNVC 426

[Close]

BLAST of Carg18456 vs. NCBI nr
Match: XP_023512794.1 (mannan endo-1,4-beta-mannosidase 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 891.7 bits (2303), Expect = 2.4e-255
Identity = 424/426 (99.53%), Postives = 425/426 (99.77%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKL DTKAPKMGFVERNGTQFMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360
           DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK
Sbjct: 301 DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSI+RLIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNVC 427
           ALKNVC
Sbjct: 421 ALKNVC 426

[Close]

BLAST of Carg18456 vs. NCBI nr
Match: XP_022985973.1 (mannan endo-1,4-beta-mannosidase 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 882.1 bits (2278), Expect = 1.9e-252
Identity = 417/426 (97.89%), Postives = 424/426 (99.53%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKL DTKAPKMGFVERNGT+FMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEG+RKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKST NPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360
           DFEDEL+FVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYD+IY+
Sbjct: 301 DFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYE 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSI+RLIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNVC 427
           ALKN+C
Sbjct: 421 ALKNMC 426

[Close]

BLAST of Carg18456 vs. ExPASy Swiss-Prot
Match: Q7Y223 (Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 2.2e-198
Identity = 319/413 (77.24%), Postives = 367/413 (88.86%), Query Frame = 0


Query: 3   GNGLFYPILGLACCLAFIYISFGD-WKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWNS 62
           GNG   PILG   C+AFIY+SFGD W  + T+  ++ FV+RNGTQF+V+G+A Y+NGWNS
Sbjct: 6   GNGPVIPILGFLTCVAFIYLSFGDLWFGLKTEG-ELAFVKRNGTQFVVDGKALYVNGWNS 65

Query: 63  YWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALD 122
           YWFMDHAV  + + RV AML A  KMGLTVCRTWAF+DGGYNALQ+ PGRF+E+VFKALD
Sbjct: 66  YWFMDHAVNDHSRHRVSAMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFKALD 125

Query: 123 HVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKN 182
           HVIAE++ HG+RLLLSLVNNLQAYGGKTQYV WAWQ+GVGLSSSNDSFF+DPSIR YFKN
Sbjct: 126 HVIAEAKTHGVRLLLSLVNNLQAYGGKTQYVNWAWQEGVGLSSSNDSFFFDPSIRRYFKN 185

Query: 183 YIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKK 242
           Y+  +LTR+NS+TGIEYRNDPTIF WELINEPRCM+D SGDTLQ+WI EM+A+IKSID K
Sbjct: 186 YLTVLLTRKNSLTGIEYRNDPTIFAWELINEPRCMSDVSGDTLQDWINEMTAFIKSIDNK 245

Query: 243 HLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQD 302
           HLLTVGLEGFYGP+SPKK TVNPE WAS LGSDF+RNS+ P+IDFASVHIYPDHWFHDQ 
Sbjct: 246 HLLTVGLEGFYGPSSPKKLTVNPERWASELGSDFVRNSDSPNIDFASVHIYPDHWFHDQG 305

Query: 303 FEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYKS 362
           FE++LKFV KWMLSHIEDGDKELKKPV+FTEFGLS+LNK + P+QRDRFY+T++DVIYKS
Sbjct: 306 FEEKLKFVVKWMLSHIEDGDKELKKPVLFTEFGLSNLNKDYDPSQRDRFYRTIFDVIYKS 365

Query: 363 AKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGG 415
           AKRKRSGAG+L WQFL+EGME  NDDFGIVPWE+ SI RL+IEQSCRL+RI G
Sbjct: 366 AKRKRSGAGTLVWQFLIEGMEGFNDDFGIVPWEQDSIQRLMIEQSCRLSRITG 417

[Close]

BLAST of Carg18456 vs. ExPASy Swiss-Prot
Match: Q9M0H6 (Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 1.4e-184
Identity = 297/417 (71.22%), Postives = 356/417 (85.37%), Query Frame = 0


Query: 10  ILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWNSYWFMDHAV 69
           ILG   C AFIY+SF D  L      K+GFV+RNGTQF+V+ +  Y+NGWNSYWFMDHAV
Sbjct: 12  ILGFFICAAFIYLSFRDLWLNHKGKAKLGFVKRNGTQFVVDDKPLYVNGWNSYWFMDHAV 71

Query: 70  EGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDHVIAESRQ 129
           + + +  V  ML A  KMGLTVCRTWAF+DGGYNALQ+ PGRF+E+VF+ALDHVIAE+R+
Sbjct: 72  DEHSRNLVGEMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFQALDHVIAEARK 131

Query: 130 HGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTR 189
           H +RLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFF+DPSIR YFKNY+K +LTR
Sbjct: 132 HDVRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFFDPSIRNYFKNYLKVLLTR 191

Query: 190 RNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLE 249
           +NS+TGIEYRNDPTIF WELINEPRC TD SG TLQ+WI+EM+ +IKSID KHLLTVGLE
Sbjct: 192 KNSVTGIEYRNDPTIFAWELINEPRCTTDVSGKTLQDWIDEMTGFIKSIDDKHLLTVGLE 251

Query: 250 GFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDFEDELKFV 309
           GFYGPNSPK  TVNPE+WAS+LG+DF++NS   +IDFASVHIYPDHWFH+Q FE++LKFV
Sbjct: 252 GFYGPNSPKGLTVNPEQWASQLGTDFVQNSNSSNIDFASVHIYPDHWFHNQTFEEKLKFV 311

Query: 310 SKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGA 369
            KWM SHIEDG KELKKPV+FTEFGLS+ NK + P+QRD+FY+ ++DV+YKSAKRK+SGA
Sbjct: 312 VKWMQSHIEDGLKELKKPVLFTEFGLSNQNKDYEPSQRDKFYRIIFDVVYKSAKRKKSGA 371

Query: 370 GSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQLKALKNVC 427
           G+L WQ  +EGME  NDDFGIVP E+ SI++L+IEQSCRL ++ G +++ K LK +C
Sbjct: 372 GTLVWQLFMEGMETFNDDFGIVPHEQDSIYKLMIEQSCRLGKVTGRLKEQK-LKELC 427

[Close]

BLAST of Carg18456 vs. ExPASy Swiss-Prot
Match: Q0JJD4 (Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 543.1 bits (1398), Expect = 2.8e-153
Identity = 260/441 (58.96%), Postives = 326/441 (73.92%), Query Frame = 0


Query: 3   GNGL-FYPILGLACCLAFIYISFGDWKLVD-------------TKAPKMGFVERNGTQFM 62
           GNGL  Y ILGLA C+A +Y S G+  L D               A  + FVER G +  
Sbjct: 4   GNGLILYHILGLASCIALVYFSLGEVDLRDALPSLPFSGGASRAAAASLPFVERRGKRLF 63

Query: 63  VNGRAFYINGWNSYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVF 122
           ++GR FYINGWNSYW MD AVE   +PRV +M R A  MGLTVCRTWAF+DG YNALQ+ 
Sbjct: 64  LDGRPFYINGWNSYWLMDLAVEPNTRPRVSSMFRTAVSMGLTVCRTWAFNDGSYNALQLS 123

Query: 123 PGRFNEQVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDS 182
           PG F+E+VFKALD V+AE+ +HG+RL+LSL NNL AYGGK QYV+WAW++GVGL++SNDS
Sbjct: 124 PGHFDERVFKALDRVVAEASEHGVRLILSLANNLDAYGGKRQYVRWAWEEGVGLTASNDS 183

Query: 183 FFYDPSIRIYFKNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWI 242
           FF+DP+IR YFK Y+KT+L R+N +TG+EYR+DPTI  WEL+NEPRC +D SGDTLQ W+
Sbjct: 184 FFFDPAIRDYFKVYLKTLLMRKNHLTGLEYRDDPTILAWELMNEPRCTSDPSGDTLQRWM 243

Query: 243 EEMSAYIKSIDKKHLLTVGLEGFYGP-NSPKKSTVNPEEW-ASRLGSDFIRNSEIPHIDF 302
           EEMSAY+KSIDKKHLLTVG EGFYGP +S +K  +NP EW  +  G+DFIRNS+I  IDF
Sbjct: 244 EEMSAYVKSIDKKHLLTVGTEGFYGPTSSQEKLNINPGEWFPNNYGADFIRNSKIQDIDF 303

Query: 303 ASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQ 362
           ASVH+YPD+W      +++LKF+++W+ +H+EDGD EL+KPV+ TEFGLS   +GF  A 
Sbjct: 304 ASVHVYPDNWLQHASLDEKLKFMTRWITAHVEDGDGELEKPVLVTEFGLSHQVEGFEDAH 363

Query: 363 RDRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQS 422
           RD  Y+ VYD+++ SA+R  +  G+L WQ   EGMEE +D F IVP ER S+ RLI EQS
Sbjct: 364 RDVLYRAVYDIVHGSARRGGAAGGALVWQLAAEGMEEYHDGFSIVPSERPSMMRLIKEQS 423

Query: 423 CRLARIG-GDIQQLKALKNVC 427
           CRLA +  G+    K LK VC
Sbjct: 424 CRLAAVRYGEEGARKVLKTVC 444

[Close]

BLAST of Carg18456 vs. ExPASy Swiss-Prot
Match: Q0DCM5 (Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN6 PE=2 SV=2)

HSP 1 Score: 419.1 bits (1076), Expect = 6.1e-116
Identity = 211/428 (49.30%), Postives = 284/428 (66.36%), Query Frame = 0


Query: 8   YPILGLACCLAFIYISF------------GDW-KL-VDTKAPKMGFVERNGTQFM--VNG 67
           Y +LGL   LA +Y+++            G W KL V    P+M F  R GT F+    G
Sbjct: 8   YSLLGLLLLLAVVYLTWFPTTHDGGGGGGGGWVKLPVPWLQPRMPFAARRGTHFVDADTG 67

Query: 68  RAFYINGWNSYWFMDHAVEGYRKPRVRA-MLRAARKMGLTVCRTWAFSDGGYNALQVFPG 127
              Y+NGWNSYW +       R P + A MLR  R+MGL+VCRTWAFSDGG  ALQ+ PG
Sbjct: 68  SPLYVNGWNSYWLLP-----ARSPALAAEMLRRGRRMGLSVCRTWAFSDGGPGALQISPG 127

Query: 128 RFNEQVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFF 187
           RF+E VF+ LD+VI E+R++ IRL+L LVNNL   GGK QYV+WA   G  +++S DSF+
Sbjct: 128 RFSEAVFQVLDYVIYEARRNHIRLILCLVNNLDNLGGKAQYVQWAQAAGANMTNSTDSFY 187

Query: 188 YDPSIRIYFKNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEE 247
             P+I+ Y+K+Y+K +LTRRNS + I Y ++P IF WEL+NEPRC++++SG  LQ WI E
Sbjct: 188 SHPTIKRYYKDYVKAILTRRNSYSRIRYSDEPAIFAWELMNEPRCVSNSSGPYLQAWIAE 247

Query: 248 MSAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVH 307
           M+AY+KS+D  HL+TVG EGFYGP   ++  VNP EWA+ L SDFI+NS + HIDFASVH
Sbjct: 248 MAAYVKSLDTNHLVTVGTEGFYGPGIAERLGVNPGEWAASLCSDFIQNSAVEHIDFASVH 307

Query: 308 IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL---SDLNKGFTPAQR 367
            YPD W      E++++++S W+ SH+ D ++ LKKPV+FTE G    SD N   T   R
Sbjct: 308 AYPDSWLPRASLEEKVRYLSNWVDSHLNDSEQILKKPVLFTEVGYLQHSDANSNST-VDR 367

Query: 368 DRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSC 416
           D   + VYD IY SA++ ++G+G+L WQ +VEG     D+F +V  +R S + LI  QSC
Sbjct: 368 DIILRIVYDKIYDSARKLQAGSGALIWQLMVEGTHMYGDNFSVVARDRPSTYSLITNQSC 427

[Close]

BLAST of Carg18456 vs. ExPASy Swiss-Prot
Match: Q9FJZ3 (Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 4.6e-108
Identity = 184/393 (46.82%), Postives = 256/393 (65.14%), Query Frame = 0


Query: 20  IYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWNSYWFMDHAVEGYRKPRVRA 79
           I I    W+L     P  GFV   G QF +NG  +Y NG+N+YW M  A +  ++ ++  
Sbjct: 12  IVIQLSCWELGTDALPSGGFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKIST 71

Query: 80  MLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDHVIAESRQHGIRLLLSLV 139
             + A + GLTV RTWAFSDGGY ALQ  PG +NE +F+ LD  +AE+R+HGI+++LS  
Sbjct: 72  AFQDASRHGLTVARTWAFSDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFA 131

Query: 140 NNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRRNSITGIEYR 199
           NN +++GG+ QYV WA   G  +SS +D FF D  ++ ++KN+IK VL R N+ T + Y+
Sbjct: 132 NNYESFGGRKQYVDWARSRGRPVSSEDD-FFTDSLVKDFYKNHIKAVLNRFNTFTKVHYK 191

Query: 200 NDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLEGFYGPNSPKK 259
           +DPTI  WEL+NEPRC +D SG  +Q WI EM+A++KS+D+ HLL  GLEGFYG +SP+ 
Sbjct: 192 DDPTIMAWELMNEPRCPSDPSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQS 251

Query: 260 STVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIED 319
            T+NP     + G+DFI N+ IP IDF +VH YPD WF D   + ++ F++KW+ +HI+D
Sbjct: 252 KTLNP---PGQFGTDFIANNRIPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQD 311

Query: 320 GDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVE 379
               L KP++  EFG S    G+TPAQRD  + TVY  IY SAKR  + AG L WQ LV 
Sbjct: 312 AQNVLHKPIILAEFGKSMKKPGYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVN 371

Query: 380 GMEESNDDFGIVPWERSSIHRLIIEQSCRLARI 413
           G++   D +GI+  + SS   +I +QS +L  I
Sbjct: 372 GIDNFQDGYGIILSQSSSTVNVISQQSRKLTLI 400

[Close]

BLAST of Carg18456 vs. ExPASy TrEMBL
Match: A0A6J1FXY1 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111448802 PE=3 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 5.3e-256
Identity = 424/426 (99.53%), Postives = 426/426 (100.00%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360
           DFE+ELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK
Sbjct: 301 DFEEELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSI+RLIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNVC 427
           ALKNVC
Sbjct: 421 ALKNVC 426

[Close]

BLAST of Carg18456 vs. ExPASy TrEMBL
Match: A0A6J1JF54 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111483854 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 9.3e-253
Identity = 417/426 (97.89%), Postives = 424/426 (99.53%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWN 60
           MAGNGLFYPILGLACCLAFIYISFGDWKL DTKAPKMGFVERNGT+FMVNGRAFYINGWN
Sbjct: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLGDTKAPKMGFVERNGTKFMVNGRAFYINGWN 60

Query: 61  SYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120
           SYWFMDHAVEG+RKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL
Sbjct: 61  SYWFMDHAVEGHRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKAL 120

Query: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180
           DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK
Sbjct: 121 DHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFK 180

Query: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240
           NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK
Sbjct: 181 NYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDK 240

Query: 241 KHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300
           KHLLTVGLEGFYGPNSPKKST NPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ
Sbjct: 241 KHLLTVGLEGFYGPNSPKKSTANPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQ 300

Query: 301 DFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYK 360
           DFEDEL+FVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYD+IY+
Sbjct: 301 DFEDELQFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDIIYE 360

Query: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQLK 420
           SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSI+RLIIEQSCRLARIGGDIQQLK
Sbjct: 361 SAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLARIGGDIQQLK 420

Query: 421 ALKNVC 427
           ALKN+C
Sbjct: 421 ALKNMC 426

[Close]

BLAST of Carg18456 vs. ExPASy TrEMBL
Match: A0A5D3C3G0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001850 PE=3 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 7.7e-239
Identity = 397/427 (92.97%), Postives = 413/427 (96.72%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKA-PKMGFVERNGTQFMVNGRAFYINGW 60
           MAGNGLFYPILG A CLAFIY+SFGD ++ DTK  P + FVERNGTQFM+NG+AFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKA 120
           NSYWFMDH+VE YRKPR+RAML+AARKMGLTVCRTWAF+DGGYNALQV PGRFNE+VFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 240
           KNYIKTVLTR+NSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPH+DFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQL 420
           KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSI++LIIEQSCRLARIGGD QQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 421 KALKNVC 427
           KALK VC
Sbjct: 421 KALKYVC 427

[Close]

BLAST of Carg18456 vs. ExPASy TrEMBL
Match: A0A1S3BJC2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 7.7e-239
Identity = 397/427 (92.97%), Postives = 413/427 (96.72%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKA-PKMGFVERNGTQFMVNGRAFYINGW 60
           MAGNGLFYPILG A CLAFIY+SFGD ++ DTK  P + FVERNGTQFM+NG+AFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKA 120
           NSYWFMDH+VE YRKPR+RAML+AARKMGLTVCRTWAF+DGGYNALQV PGRFNE+VFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 240
           KNYIKTVLTR+NSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPH+DFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQL 420
           KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSI++LIIEQSCRLARIGGD QQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 421 KALKNVC 427
           KALK VC
Sbjct: 421 KALKYVC 427

[Close]

BLAST of Carg18456 vs. ExPASy TrEMBL
Match: A0A0A0KB35 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 3.8e-238
Identity = 395/427 (92.51%), Postives = 412/427 (96.49%), Query Frame = 0


Query: 1   MAGNGLFYPILGLACCLAFIYISFGDWKLVDTKA-PKMGFVERNGTQFMVNGRAFYINGW 60
           MAGNGLFYPILG A CLAFIY+SFGD ++ DTK  P + FVERNGTQFM+NG+AFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKA 120
           NSYWFMDH+VE YRKPR+RAML+AARKMGLTVCRTWAF+DGGYNALQV PGRFNE+VFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 240
           KNY+KTVLTR+NSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 181 KNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI H+DFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSFYKTVYDVIY 360

Query: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQL 420
           KSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSI++LIIEQSCRLARIGGD QQL
Sbjct: 361 KSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 421 KALKNVC 427
           KALK VC
Sbjct: 421 KALKYVC 427

[Close]

BLAST of Carg18456 vs. TAIR 10
Match: AT2G20680.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 693.0 bits (1787), Expect = 1.5e-199
Identity = 319/413 (77.24%), Postives = 367/413 (88.86%), Query Frame = 0


Query: 3   GNGLFYPILGLACCLAFIYISFGD-WKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWNS 62
           GNG   PILG   C+AFIY+SFGD W  + T+  ++ FV+RNGTQF+V+G+A Y+NGWNS
Sbjct: 6   GNGPVIPILGFLTCVAFIYLSFGDLWFGLKTEG-ELAFVKRNGTQFVVDGKALYVNGWNS 65

Query: 63  YWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALD 122
           YWFMDHAV  + + RV AML A  KMGLTVCRTWAF+DGGYNALQ+ PGRF+E+VFKALD
Sbjct: 66  YWFMDHAVNDHSRHRVSAMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFKALD 125

Query: 123 HVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKN 182
           HVIAE++ HG+RLLLSLVNNLQAYGGKTQYV WAWQ+GVGLSSSNDSFF+DPSIR YFKN
Sbjct: 126 HVIAEAKTHGVRLLLSLVNNLQAYGGKTQYVNWAWQEGVGLSSSNDSFFFDPSIRRYFKN 185

Query: 183 YIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKK 242
           Y+  +LTR+NS+TGIEYRNDPTIF WELINEPRCM+D SGDTLQ+WI EM+A+IKSID K
Sbjct: 186 YLTVLLTRKNSLTGIEYRNDPTIFAWELINEPRCMSDVSGDTLQDWINEMTAFIKSIDNK 245

Query: 243 HLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQD 302
           HLLTVGLEGFYGP+SPKK TVNPE WAS LGSDF+RNS+ P+IDFASVHIYPDHWFHDQ 
Sbjct: 246 HLLTVGLEGFYGPSSPKKLTVNPERWASELGSDFVRNSDSPNIDFASVHIYPDHWFHDQG 305

Query: 303 FEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYKS 362
           FE++LKFV KWMLSHIEDGDKELKKPV+FTEFGLS+LNK + P+QRDRFY+T++DVIYKS
Sbjct: 306 FEEKLKFVVKWMLSHIEDGDKELKKPVLFTEFGLSNLNKDYDPSQRDRFYRTIFDVIYKS 365

Query: 363 AKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGG 415
           AKRKRSGAG+L WQFL+EGME  NDDFGIVPWE+ SI RL+IEQSCRL+RI G
Sbjct: 366 AKRKRSGAGTLVWQFLIEGMEGFNDDFGIVPWEQDSIQRLMIEQSCRLSRITG 417

[Close]

BLAST of Carg18456 vs. TAIR 10
Match: AT4G28320.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 647.1 bits (1668), Expect = 9.7e-186
Identity = 297/417 (71.22%), Postives = 356/417 (85.37%), Query Frame = 0


Query: 10  ILGLACCLAFIYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWNSYWFMDHAV 69
           ILG   C AFIY+SF D  L      K+GFV+RNGTQF+V+ +  Y+NGWNSYWFMDHAV
Sbjct: 12  ILGFFICAAFIYLSFRDLWLNHKGKAKLGFVKRNGTQFVVDDKPLYVNGWNSYWFMDHAV 71

Query: 70  EGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDHVIAESRQ 129
           + + +  V  ML A  KMGLTVCRTWAF+DGGYNALQ+ PGRF+E+VF+ALDHVIAE+R+
Sbjct: 72  DEHSRNLVGEMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFQALDHVIAEARK 131

Query: 130 HGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTR 189
           H +RLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFF+DPSIR YFKNY+K +LTR
Sbjct: 132 HDVRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFFDPSIRNYFKNYLKVLLTR 191

Query: 190 RNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLE 249
           +NS+TGIEYRNDPTIF WELINEPRC TD SG TLQ+WI+EM+ +IKSID KHLLTVGLE
Sbjct: 192 KNSVTGIEYRNDPTIFAWELINEPRCTTDVSGKTLQDWIDEMTGFIKSIDDKHLLTVGLE 251

Query: 250 GFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDFEDELKFV 309
           GFYGPNSPK  TVNPE+WAS+LG+DF++NS   +IDFASVHIYPDHWFH+Q FE++LKFV
Sbjct: 252 GFYGPNSPKGLTVNPEQWASQLGTDFVQNSNSSNIDFASVHIYPDHWFHNQTFEEKLKFV 311

Query: 310 SKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGA 369
            KWM SHIEDG KELKKPV+FTEFGLS+ NK + P+QRD+FY+ ++DV+YKSAKRK+SGA
Sbjct: 312 VKWMQSHIEDGLKELKKPVLFTEFGLSNQNKDYEPSQRDKFYRIIFDVVYKSAKRKKSGA 371

Query: 370 GSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGGDIQQLKALKNVC 427
           G+L WQ  +EGME  NDDFGIVP E+ SI++L+IEQSCRL ++ G +++ K LK +C
Sbjct: 372 GTLVWQLFMEGMETFNDDFGIVPHEQDSIYKLMIEQSCRLGKVTGRLKEQK-LKELC 427

[Close]

BLAST of Carg18456 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 392.9 bits (1008), Expect = 3.3e-109
Identity = 184/393 (46.82%), Postives = 256/393 (65.14%), Query Frame = 0


Query: 20  IYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWNSYWFMDHAVEGYRKPRVRA 79
           I I    W+L     P  GFV   G QF +NG  +Y NG+N+YW M  A +  ++ ++  
Sbjct: 12  IVIQLSCWELGTDALPSGGFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKIST 71

Query: 80  MLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDHVIAESRQHGIRLLLSLV 139
             + A + GLTV RTWAFSDGGY ALQ  PG +NE +F+ LD  +AE+R+HGI+++LS  
Sbjct: 72  AFQDASRHGLTVARTWAFSDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFA 131

Query: 140 NNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRRNSITGIEYR 199
           NN +++GG+ QYV WA   G  +SS +D FF D  ++ ++KN+IK VL R N+ T + Y+
Sbjct: 132 NNYESFGGRKQYVDWARSRGRPVSSEDD-FFTDSLVKDFYKNHIKAVLNRFNTFTKVHYK 191

Query: 200 NDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLEGFYGPNSPKK 259
           +DPTI  WEL+NEPRC +D SG  +Q WI EM+A++KS+D+ HLL  GLEGFYG +SP+ 
Sbjct: 192 DDPTIMAWELMNEPRCPSDPSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQS 251

Query: 260 STVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIED 319
            T+NP     + G+DFI N+ IP IDF +VH YPD WF D   + ++ F++KW+ +HI+D
Sbjct: 252 KTLNP---PGQFGTDFIANNRIPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQD 311

Query: 320 GDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVE 379
               L KP++  EFG S    G+TPAQRD  + TVY  IY SAKR  + AG L WQ LV 
Sbjct: 312 AQNVLHKPIILAEFGKSMKKPGYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVN 371

Query: 380 GMEESNDDFGIVPWERSSIHRLIIEQSCRLARI 413
           G++   D +GI+  + SS   +I +QS +L  I
Sbjct: 372 GIDNFQDGYGIILSQSSSTVNVISQQSRKLTLI 400

[Close]

BLAST of Carg18456 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 386.3 bits (991), Expect = 3.1e-107
Identity = 177/371 (47.71%), Postives = 257/371 (69.27%), Query Frame = 0


Query: 40  VERNGTQFMVNGRAFYINGWNSYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSD 99
           V+R G QF +NG+ FY+NG+N+YW M  A +   + +V  + + A  +G+TV RTWAF+D
Sbjct: 48  VQRKGMQFTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGRTWAFND 107

Query: 100 GGYNALQVFPGRFNEQVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDG 159
           G + ALQ  P  ++E+VFKALD V++E+R++ IRL+LSLVNN  AYGGK QYVKW    G
Sbjct: 108 GQWRALQKSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVKWGNASG 167

Query: 160 VGLSSSNDSFFYDPSIRIYFKNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDA 219
           + L +S+D FF +P++R +++++++TVL R N+ T I Y+NDPTIF WEL+NEPRC +D 
Sbjct: 168 LNL-TSDDDFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPRCPSDP 227

Query: 220 SGDTLQEWIEEMSAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNS 279
           SGD LQ WI+EM+ ++KS+D KHL+ +GLEGFYGP++P ++  NP  +A+++G+DFIRN+
Sbjct: 228 SGDKLQSWIQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTDFIRNN 287

Query: 280 EIPHIDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLN 339
           ++  IDFASVH+YPD W         L+F S WM +H+ED +  L  PV+FTEFG+S  +
Sbjct: 288 QVLGIDFASVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFGVSAHD 347

Query: 340 KGFTPAQRDRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIH 399
            GF  + RD    TVY +   S ++  +GAGSL WQ   +G E  +D + +      +  
Sbjct: 348 PGFNTSFRDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTRAHTAS 407

Query: 400 RLIIEQSCRLA 411
           ++I  QS RLA
Sbjct: 408 KIISLQSKRLA 417

[Close]

BLAST of Carg18456 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 360.1 bits (923), Expect = 2.4e-99
Identity = 174/405 (42.96%), Postives = 255/405 (62.96%), Query Frame = 0


Query: 16  CLAF-----IYISFGDWKLVDTKAPKMGFVERNGTQFMVNGRAFYINGWNSYWFMDHAVE 75
           CL F     I I+     L    A   GFV R G QF++NG+ FY NG+N+YW    A +
Sbjct: 3   CLCFVVLLAILIAQNSSDLGVKSASSDGFVSRKGVQFILNGKPFYANGFNAYWLAYEATD 62

Query: 76  GYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQVFPGRFNEQVFKALDHVIAESRQH 135
              + ++  + + A    LT+ RTW F DGGY ALQ+ PG ++E+ F+ LD  IAE+++ 
Sbjct: 63  STTRFKITYVFQNATIHDLTIVRTWGFRDGGYRALQIAPGVYDEKTFQGLDFAIAEAKRL 122

Query: 136 GIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRR 195
           GI+++++ VNN   +GG+ QYV WA   G  + SS+D F+ +P ++ Y+KN++KT++ R 
Sbjct: 123 GIKMIITFVNNYSDFGGRKQYVDWAKNTGQNV-SSDDDFYTNPLVKQYYKNHVKTMVNRV 182

Query: 196 NSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLEG 255
           N+ T +EY+++PTI GWEL+NEP+C  D SG TL  W+ EM+ Y+KS+D KHLL+ GLEG
Sbjct: 183 NTFTKVEYKDEPTIMGWELMNEPQCRADPSGKTLTAWMNEMALYVKSVDSKHLLSTGLEG 242

Query: 256 FYGPNSP-KKSTVNPEEWASRLGSDFIRNSEIPHIDFASVHIYPDHWFHDQDFEDELKFV 315
           FYG +SP +K+++NP   A+ LG+DFI N ++  IDFAS+H YPD WF + D +  L  +
Sbjct: 243 FYGDSSPQRKTSLNPVA-ANVLGTDFIANHKLDAIDFASIHSYPDLWFPNLDEKSRLNLL 302

Query: 316 SKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGA 375
            KW+  H+ED    LKKP++  EFG      G+T AQRD  +   +D IY+SA++    A
Sbjct: 303 RKWLECHLEDAQNILKKPLILGEFGKPTNTPGYTQAQRDAVFNATFDTIYESAEKGGPAA 362

Query: 376 GSLAWQFLVEGMEESNDDFGIVPWERSSIHRLIIEQSCRLARIGG 415
           G+L W  + +GM    D   IV  E S+   +I E+S +L  I G
Sbjct: 363 GALFWHVISDGMNNFKDPLSIVLSENSTTVNIITEESRKLGLIRG 405

The following BLAST results are available for this feature:

BLAST of Carg18456 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010376.1	7.6e-257	100.00	Mannan endo-1,4-beta-mannosidase 2, partial [Cucurbita argyrosperma subsp. argyr...	[more]
XP_022944323.1	1.1e-255	99.53	mannan endo-1,4-beta-mannosidase 2-like isoform X1 [Cucurbita moschata]	[more]
KAG6570514.1	1.1e-255	99.53	Mannan endo-1,4-beta-mannosidase 2, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_023512794.1	2.4e-255	99.53	mannan endo-1,4-beta-mannosidase 2-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022985973.1	1.9e-252	97.89	mannan endo-1,4-beta-mannosidase 2-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg18456 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7Y223	2.2e-198	77.24	Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 ...	[more]
Q9M0H6	1.4e-184	71.22	Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 ...	[more]
Q0JJD4	2.8e-153	58.96	Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q0DCM5	6.1e-116	49.30	Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q9FJZ3	4.6e-108	46.82	Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 ...	[more]

BLAST of Carg18456 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FXY1	5.3e-256	99.53	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111448802 P...	[more]
A0A6J1JF54	9.3e-253	97.89	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111483854 PE=...	[more]
A0A5D3C3G0	7.7e-239	92.97	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BJC2	7.7e-239	92.97	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV...	[more]
A0A0A0KB35	3.8e-238	92.51	Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3...	[more]

BLAST of Carg18456 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G20680.1	1.5e-199	77.24	Glycosyl hydrolase superfamily protein	[more]
AT4G28320.1	9.7e-186	71.22	Glycosyl hydrolase superfamily protein	[more]
AT5G66460.1	3.3e-109	46.82	Glycosyl hydrolase superfamily protein	[more]
AT5G01930.1	3.1e-107	47.71	Glycosyl hydrolase superfamily protein	[more]
AT3G10890.1	2.4e-99	42.96	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 56..350 e-value: 5.6E-15 score: 55.5
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 35..413 e-value: 4.1E-134 score: 449.2
None	No IPR available	PANTHER	PTHR31451:SF61	BNAA09G43620D PROTEIN	coord: 5..426
IPR045053	Mannan endo-1,4-beta-mannosidase-like	PANTHER	PTHR31451	FAMILY NOT NAMED	coord: 5..426
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 38..397

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18456-RA	Carg18456-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds