Carg18160 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg18160
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionserine/threonine-protein kinase AtPK2/AtPK19-like
LocationCarg_Chr11: 688792 .. 691655 (-)
RNA-Seq ExpressionCarg18160
SyntenyCarg18160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGTATGCATCTACTCATAACAAGAACATGCACGCCCTTCTAACAGCCAAACTCACCAAATTGACTCTCCCTTCCTCTTCTTCTTCTTCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTTCTTCATCCACGGCCGCCCTTCTTGACTTCGACTTCTCTGACGTATTCGGCCCCACAAATTCCTCTTTACCATCTTTCCCCGGGGACCCACAGCACAATGAACAACAACCAGCGGTCATTCATAATAGATCTCACTCATTTGTGGGTCCGTCTCCACGGTTAACTCCCCCGTCTTCCCTTTCTTTTTTCCGGGAAGTTAAATCACAGATCGAGGAGGAAGAAGATGACCTAGAAATCTCGACTGGCGATGGAGTTCAAAAGGAGAAGGGGGCGGATGAGAGCGAGAATGACGGTGGAGAAAATTCAGAGGGGAAGGTTGGAGTTGGGGATTTCGATATTCTTCGAGTTGTGGGAAAAGGGGCGTTTGGGAAGGTCTTTCTAGTGAGGAAGAAAGGGGATTCGAAAGGAAACGGTCCCAATAATGATGGGATTTACGCCATGAAAGTGATGAGAAAGGATACCATTATAAAGAAGAATCATGTGGATTATATGAAGGCTGAGAGAGATATTCTCACAAAAGTTTCGCACCCTTTCATTGTTCAACTCCGCTACTCTTTCCAGGTTCTAACATTTCTTTCTCATCTACCTCCTTGATTGCTTAGACGAACTTGTATTTGCTTATCTTTGCGTGTTTGTTTTGGTTCGCAGTCCAAATCAAAACTCTATTTGATTCTTGATTTCATAAATGGGGGGCATCTGTTCTTCCATCTGTACCGGCAGGGAATCTTTAGGTACCTTCCAATCTCAGTTAGGAACTTGTTTTTGATACTTTAGGAACATTGTTTGATCTGGATTATGGGTTGTTTTGATTAGTGAGGATCAGGCAAGGGTCTATGCTGCTGAGATCGTGTCAGCTGTTTCGCATCTTCATGACTGCGGCATTGTGCACCGGGATCTCAAGCCTGAGAACATTCTTATGGATGCTGATGGCCATGTAATGTCATGGCTTTCTTCTTAAATTGTACAAACTAATGAATGTTCTTCCTGTGTTCCTGAATCTTGAATGTTTGTGCAGGTTGTCCTTACGGATTTTGGACTGGCAAAGGAAATTGATGAATCGAGTCGGTCCAATTCGTTGTGTGGAACAACAGAATACATGGCTCCGGAAATCTTGCTTTCTAAAGGTCACAACAAGGATGCTGATTGGTGGAGCGTCGGAGTTCTTTTGTATGAGATGCTTAGTGGCAAGGTACGTGAATGGCAAGTCTGTCCTAAGAATGAGAACTATAGGAATTTCAATGAACCTAGTTTAGAAACTCAACCTCATTTGTGATGTTTGGTGTGAGATCCCACGTCGGTTGGAGAGGGGAACGAAACATTTGCTTATAAGGGTGTGGAAACCTCTTTCTAGCAACAATAGCCTCTTTCTAGCAACAATAGCGCTTATAAGGGTGTGGAAACCTCTTTCTAGCAACAATAGTGAGGCTGACGACAATAGGGTCAAAATGGACAATATTTGCTAGCAGTGGGTTGTTAGACTTAGACACTAGGCAGTGCCAATGAGGACACTGGCTCCCAATGGGGGTGGATTGTGAAATCCTATATCGGTTAGAGAGGGAAACGAAACATTCCTTGTAAGGGCGTGAAAACTTCTTCCTAACAAACGCATTTGAAAATTGTGAGGCGTGATATGTAACGGGTCAAAACAGACAATATCTACTAGCGGTAGGCTTGGTCTGTTATAAATGGTATCAGAGCTAGACACTGGTCAGTGTGCTAGCGAGGACATTACGTCCCCCAATGGGGTGGATTGTGAGATCCCATATCGGTTGGAGAGCGGAACGAAACATTATTTATAAATGTATGAAAACCTCTTCTTAACATACGCGTTTTAAAACTGTGAGGCTAACGACGACACGTAACGGACCAAAGCGGACAATATTTGCTAGCGGTGAGCTTGGACTATTAGACTTGGAAATTTGGACTACTAGACTTGGCAACTCTGAATTGGAGTCCATTCATTATCACAATGCAAAGGCTTGTTTTTATTGTGGCTGCTGATGTTTGCTTATGTTTTTTACTATATGCAGCCACCATTCACACACGCTAATAGAAAGAAGCTTCAAGAGAGAATAATCAAAGAGAAAGTGAAACTTCCATCATACCTGAGCACTGAAGCTCACTCTTTGCTCAGAGGGGTAAGCTGAGAGAGAACTCCATTTTCGTCTGTAGTAATCTTTATTTTCATGACCGATACAATGGGAGTTCTTGTTCTTCACAGCTATTACAAAAGGATCCTTCTAAAAGATTGGGAAGTGGACCCAACAGAGGGGCGGACATAAAAAATCACAAATGGTTTCGACCTGTGAACTGGAAGAAATTGGAATGTAGGGAGCTGCAACCAAAATTCAAGCCGGATGTCAAGGGAAATGACTGCACAGCAAATTTTGATAATTGTTGGACGACTATGCCCCCCGACGACTCGCCAGCTCCAACGCCAACCGACGGAGGACACTTCCACGGATACTCGTATGTTGCTCCAGACCCTTGGCTTTCATCATCTGGATAGATAGATTGGAGATGAGGACGGGGGAATCTTCTTATTATACTATACTATACTGGTAAATGCAACTGTATGTACATCTCCTATACTATACTGCCATTAAGTTATTGCCTACTTCACAATCTCTCCGGTAGCTTCAAATATGTATGCTAAGCTTTCACGTTCTTTCTCTGTCTCACCATGTGATCAAAAAGTATTGGATATGATATGAACAACGCTGCATTTCTTTTTACAT

mRNA sequence

ATGGTGTATGCATCTACTCATAACAAGAACATGCACGCCCTTCTAACAGCCAAACTCACCAAATTGACTCTCCCTTCCTCTTCTTCTTCTTCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTTCTTCATCCACGGCCGCCCTTCTTGACTTCGACTTCTCTGACGTATTCGGCCCCACAAATTCCTCTTTACCATCTTTCCCCGGGGACCCACAGCACAATGAACAACAACCAGCGGTCATTCATAATAGATCTCACTCATTTGTGGGTCCGTCTCCACGGTTAACTCCCCCGTCTTCCCTTTCTTTTTTCCGGGAAGTTAAATCACAGATCGAGGAGGAAGAAGATGACCTAGAAATCTCGACTGGCGATGGAGTTCAAAAGGAGAAGGGGGCGGATGAGAGCGAGAATGACGGTGGAGAAAATTCAGAGGGGAAGGTTGGAGTTGGGGATTTCGATATTCTTCGAGTTGTGGGAAAAGGGGCGTTTGGGAAGGTCTTTCTAGTGAGGAAGAAAGGGGATTCGAAAGGAAACGGTCCCAATAATGATGGGATTTACGCCATGAAAGTGATGAGAAAGGATACCATTATAAAGAAGAATCATGTGGATTATATGAAGGCTGAGAGAGATATTCTCACAAAAGTTTCGCACCCTTTCATTGTTCAACTCCGCTACTCTTTCCAGTCCAAATCAAAACTCTATTTGATTCTTGATTTCATAAATGGGGGGCATCTGTTCTTCCATCTGTACCGGCAGGGAATCTTTAGTGAGGATCAGGCAAGGGTCTATGCTGCTGAGATCGTGTCAGCTGTTTCGCATCTTCATGACTGCGGCATTGTGCACCGGGATCTCAAGCCTGAGAACATTCTTATGGATGCTGATGGCCATGTTGTCCTTACGGATTTTGGACTGGCAAAGGAAATTGATGAATCGAGTCGGTCCAATTCGTTGTGTGGAACAACAGAATACATGGCTCCGGAAATCTTGCTTTCTAAAGGTCACAACAAGGATGCTGATTGGTGGAGCGTCGGAGTTCTTTTGTATGAGATGCTTAGTGGCAAGCCACCATTCACACACGCTAATAGAAAGAAGCTTCAAGAGAGAATAATCAAAGAGAAAGTGAAACTTCCATCATACCTGAGCACTGAAGCTCACTCTTTGCTCAGAGGGCTATTACAAAAGGATCCTTCTAAAAGATTGGGAAGTGGACCCAACAGAGGGGCGGACATAAAAAATCACAAATGGTTTCGACCTGTGAACTGGAAGAAATTGGAATGTAGGGAGCTGCAACCAAAATTCAAGCCGGATGTCAAGGGAAATGACTGCACAGCAAATTTTGATAATTGTTGGACGACTATGCCCCCCGACGACTCGCCAGCTCCAACGCCAACCGACGGAGGACACTTCCACGGATACTCGTATGTTGCTCCAGACCCTTGGCTTTCATCATCTGGATAGATAGATTGGAGATGAGGACGGGGGAATCTTCTTATTATACTATACTATACTGGTAAATGCAACTGTATGTACATCTCCTATACTATACTGCCATTAAGTTATTGCCTACTTCACAATCTCTCCGGTAGCTTCAAATATGTATGCTAAGCTTTCACGTTCTTTCTCTGTCTCACCATGTGATCAAAAAGTATTGGATATGATATGAACAACGCTGCATTTCTTTTTACAT

Coding sequence (CDS)

ATGGTGTATGCATCTACTCATAACAAGAACATGCACGCCCTTCTAACAGCCAAACTCACCAAATTGACTCTCCCTTCCTCTTCTTCTTCTTCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTTCTTCATCCACGGCCGCCCTTCTTGACTTCGACTTCTCTGACGTATTCGGCCCCACAAATTCCTCTTTACCATCTTTCCCCGGGGACCCACAGCACAATGAACAACAACCAGCGGTCATTCATAATAGATCTCACTCATTTGTGGGTCCGTCTCCACGGTTAACTCCCCCGTCTTCCCTTTCTTTTTTCCGGGAAGTTAAATCACAGATCGAGGAGGAAGAAGATGACCTAGAAATCTCGACTGGCGATGGAGTTCAAAAGGAGAAGGGGGCGGATGAGAGCGAGAATGACGGTGGAGAAAATTCAGAGGGGAAGGTTGGAGTTGGGGATTTCGATATTCTTCGAGTTGTGGGAAAAGGGGCGTTTGGGAAGGTCTTTCTAGTGAGGAAGAAAGGGGATTCGAAAGGAAACGGTCCCAATAATGATGGGATTTACGCCATGAAAGTGATGAGAAAGGATACCATTATAAAGAAGAATCATGTGGATTATATGAAGGCTGAGAGAGATATTCTCACAAAAGTTTCGCACCCTTTCATTGTTCAACTCCGCTACTCTTTCCAGTCCAAATCAAAACTCTATTTGATTCTTGATTTCATAAATGGGGGGCATCTGTTCTTCCATCTGTACCGGCAGGGAATCTTTAGTGAGGATCAGGCAAGGGTCTATGCTGCTGAGATCGTGTCAGCTGTTTCGCATCTTCATGACTGCGGCATTGTGCACCGGGATCTCAAGCCTGAGAACATTCTTATGGATGCTGATGGCCATGTTGTCCTTACGGATTTTGGACTGGCAAAGGAAATTGATGAATCGAGTCGGTCCAATTCGTTGTGTGGAACAACAGAATACATGGCTCCGGAAATCTTGCTTTCTAAAGGTCACAACAAGGATGCTGATTGGTGGAGCGTCGGAGTTCTTTTGTATGAGATGCTTAGTGGCAAGCCACCATTCACACACGCTAATAGAAAGAAGCTTCAAGAGAGAATAATCAAAGAGAAAGTGAAACTTCCATCATACCTGAGCACTGAAGCTCACTCTTTGCTCAGAGGGCTATTACAAAAGGATCCTTCTAAAAGATTGGGAAGTGGACCCAACAGAGGGGCGGACATAAAAAATCACAAATGGTTTCGACCTGTGAACTGGAAGAAATTGGAATGTAGGGAGCTGCAACCAAAATTCAAGCCGGATGTCAAGGGAAATGACTGCACAGCAAATTTTGATAATTGTTGGACGACTATGCCCCCCGACGACTCGCCAGCTCCAACGCCAACCGACGGAGGACACTTCCACGGATACTCGTATGTTGCTCCAGACCCTTGGCTTTCATCATCTGGATAG

Protein sequence

MVYASTHNKNMHALLTAKLTKLTLPSSSSSSPPPPPPPPPPPSSSTAALLDFDFSDVFGPTNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLEISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVLTDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTHANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPVNWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDPWLSSSG
Homology
BLAST of Carg18160 vs. NCBI nr
Match: KAG7021487.1 (Serine/threonine-protein kinase AtPK2/AtPK19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 983.0 bits (2540), Expect = 9.1e-283
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 0

Query: 1   MVYASTHNKNMHALLTAKLTKLTLPSSSSSSPPPPPPPPPPPSSSTAALLDFDFSDVFGP 60
           MVYASTHNKNMHALLTAKLTKLTLPSSSSSSPPPPPPPPPPPSSSTAALLDFDFSDVFGP
Sbjct: 1   MVYASTHNKNMHALLTAKLTKLTLPSSSSSSPPPPPPPPPPPSSSTAALLDFDFSDVFGP 60

Query: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120
           TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE
Sbjct: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120

Query: 121 ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180
           ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG
Sbjct: 121 ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180

Query: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240
           PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF
Sbjct: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240

Query: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL 300
           INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL
Sbjct: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL 300

Query: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360
           TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH
Sbjct: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360

Query: 361 ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420
           ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV
Sbjct: 361 ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420

Query: 421 NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP 480
           NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP
Sbjct: 421 NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP 480

Query: 481 WLSSSG 487
           WLSSSG
Sbjct: 481 WLSSSG 486

BLAST of Carg18160 vs. NCBI nr
Match: KAG6587502.1 (Serine/threonine-protein kinase AtPK2/AtPK19, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 976.9 bits (2524), Expect = 6.6e-281
Identity = 484/486 (99.59%), Postives = 484/486 (99.59%), Query Frame = 0

Query: 1   MVYASTHNKNMHALLTAKLTKLTLPSSSSSSPPPPPPPPPPPSSSTAALLDFDFSDVFGP 60
           MVYASTHNKNMHALLTAKLTKLTLPSSSSSS PPPPPPPPPPSSSTAALLDFDFSDVFGP
Sbjct: 1   MVYASTHNKNMHALLTAKLTKLTLPSSSSSSSPPPPPPPPPPSSSTAALLDFDFSDVFGP 60

Query: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120
           TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE
Sbjct: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120

Query: 121 ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180
           ISTGD VQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG
Sbjct: 121 ISTGDEVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180

Query: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240
           PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF
Sbjct: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240

Query: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL 300
           INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL
Sbjct: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL 300

Query: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360
           TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH
Sbjct: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360

Query: 361 ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420
           ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV
Sbjct: 361 ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420

Query: 421 NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP 480
           NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP
Sbjct: 421 NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP 480

Query: 481 WLSSSG 487
           WLSSSG
Sbjct: 481 WLSSSG 486

BLAST of Carg18160 vs. NCBI nr
Match: XP_022933334.1 (serine/threonine-protein kinase AtPK1/AtPK6-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 974.2 bits (2517), Expect = 4.2e-280
Identity = 484/486 (99.59%), Postives = 484/486 (99.59%), Query Frame = 0

Query: 1   MVYASTHNKNMHALLTAKLTKLTLPSSSSSSPPPPPPPPPPPSSSTAALLDFDFSDVFGP 60
           MVYASTHNKNMHALLTAKLTKLTLP SSSSSPPPPPPPPPP SSSTAALLDFDFSDVFGP
Sbjct: 1   MVYASTHNKNMHALLTAKLTKLTLP-SSSSSPPPPPPPPPPSSSSTAALLDFDFSDVFGP 60

Query: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120
           TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE
Sbjct: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120

Query: 121 ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180
           ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG
Sbjct: 121 ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180

Query: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240
           PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF
Sbjct: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240

Query: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL 300
           INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL
Sbjct: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL 300

Query: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360
           TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH
Sbjct: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360

Query: 361 ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420
           ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV
Sbjct: 361 ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420

Query: 421 NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP 480
           NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP
Sbjct: 421 NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP 480

Query: 481 WLSSSG 487
           WLSSSG
Sbjct: 481 WLSSSG 485

BLAST of Carg18160 vs. NCBI nr
Match: XP_022972650.1 (serine/threonine-protein kinase AtPK2/AtPK19-like [Cucurbita maxima])

HSP 1 Score: 961.4 bits (2484), Expect = 2.8e-276
Identity = 479/486 (98.56%), Postives = 480/486 (98.77%), Query Frame = 0

Query: 1   MVYASTHNKNMHALLTAKLTKLTLPSSSSSSPPPPPPPPPPPSSSTAALLDFDFSDVFGP 60
           MVYASTHNKNMHALLTAKLTKLTLPSSSSS   PPPPPPPPPSSSTAALLDFDFSDVFGP
Sbjct: 1   MVYASTHNKNMHALLTAKLTKLTLPSSSSS---PPPPPPPPPSSSTAALLDFDFSDVFGP 60

Query: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120
           TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE
Sbjct: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120

Query: 121 ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180
           ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG
Sbjct: 121 ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180

Query: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240
           PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF
Sbjct: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240

Query: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL 300
           INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL
Sbjct: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL 300

Query: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360
           TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH
Sbjct: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360

Query: 361 ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420
           ANRKKLQERIIKEKVKLP YLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV
Sbjct: 361 ANRKKLQERIIKEKVKLPPYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420

Query: 421 NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP 480
           NWKKLE RELQPKFKPDVKGNDCTANFDNCWTTM PDDSPAPTPT+GGHFHGYSYVAPDP
Sbjct: 421 NWKKLEYRELQPKFKPDVKGNDCTANFDNCWTTMAPDDSPAPTPTNGGHFHGYSYVAPDP 480

Query: 481 WLSSSG 487
           WLSSSG
Sbjct: 481 WLSSSG 483

BLAST of Carg18160 vs. NCBI nr
Match: XP_023531988.1 (serine/threonine-protein kinase AtPK2/AtPK19-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 959.5 bits (2479), Expect = 1.1e-275
Identity = 479/486 (98.56%), Postives = 479/486 (98.56%), Query Frame = 0

Query: 1   MVYASTHNKNMHALLTAKLTKLTLPSSSSSSPPPPPPPPPPPSSSTAALLDFDFSDVFGP 60
           MVYAST NKNMHALLTAKLTKLTLPSSSSSS    PPPPPPPSSSTAALLDFDFSDVFGP
Sbjct: 1   MVYASTPNKNMHALLTAKLTKLTLPSSSSSS---SPPPPPPPSSSTAALLDFDFSDVFGP 60

Query: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120
           TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE
Sbjct: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120

Query: 121 ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180
           ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG
Sbjct: 121 ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180

Query: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240
           PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF
Sbjct: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240

Query: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL 300
           INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHD GIVHRDLKPENILMDADGHVVL
Sbjct: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDSGIVHRDLKPENILMDADGHVVL 300

Query: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360
           TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH
Sbjct: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360

Query: 361 ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420
           ANRKKLQERIIKEKVKLP YLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV
Sbjct: 361 ANRKKLQERIIKEKVKLPPYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420

Query: 421 NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP 480
           NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP
Sbjct: 421 NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP 480

Query: 481 WLSSSG 487
           WLSSSG
Sbjct: 481 WLSSSG 483

BLAST of Carg18160 vs. ExPASy Swiss-Prot
Match: Q39030 (Serine/threonine-protein kinase AtPK2/AtPK19 OS=Arabidopsis thaliana OX=3702 GN=ATPK2 PE=1 SV=2)

HSP 1 Score: 503.8 bits (1296), Expect = 2.1e-141
Identity = 258/445 (57.98%), Postives = 326/445 (73.26%), Query Frame = 0

Query: 41  PPSSSTAALLDFDFSDVFGPTNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPS 100
           PP S     L+  FSDVFGP   +      D  ++E  PAV+++RSHS VGPS  L    
Sbjct: 28  PPKSVLGDNLELQFSDVFGPMPEANSEEACDVAYDE--PAVVYSRSHSLVGPS--LVVSH 87

Query: 101 SLSFFREVKSQIEEEEDDLEISTGDGVQKE---KGADESEND-GGENSEGKVGVGDFDIL 160
           SL   +    + E+  D +E   G+ +++     G D+++++   E   G VG+ DF++L
Sbjct: 88  SLKMNKLTLRETEDSVDLVECVEGESIKENDEFSGNDDTDSEKSPEEVSGVVGIEDFEVL 147

Query: 161 RVVGKGAFGKVFLVRKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKV 220
           +VVG+GAFGKV+ VRKK  S+        IYAMKVMRKD I++KNH +YMKAERDILTK+
Sbjct: 148 KVVGQGAFGKVYQVRKKDTSE--------IYAMKVMRKDKIVEKNHAEYMKAERDILTKI 207

Query: 221 SHPFIVQLRYSFQSKSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLH 280
            HPFIVQL+YSFQ+K +LYL+LDFINGGHLFF LY QG+F ED ARVY AEIVSAVSHLH
Sbjct: 208 DHPFIVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHQGLFREDLARVYTAEIVSAVSHLH 267

Query: 281 DCGIVHRDLKPENILMDADGHVVLTDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHN 340
           + GI+HRDLKPENILMD DGHV+LTDFGLAKE +E++RSNS+CGTTEYMAPEI+  KGH+
Sbjct: 268 EKGIMHRDLKPENILMDVDGHVMLTDFGLAKEFEENTRSNSMCGTTEYMAPEIVRGKGHD 327

Query: 341 KDADWWSVGVLLYEMLSGKPPFTHANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKD 400
           K ADWWSVG+LLYEML+GKPPF   ++ K+Q++I+K+K+KLP +LS EAH+LL+GLLQK+
Sbjct: 328 KAADWWSVGILLYEMLTGKPPFL-GSKGKIQQKIVKDKIKLPQFLSNEAHALLKGLLQKE 387

Query: 401 PSKRLGSGPNRGADIKNHKWFRPVNWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPP 460
           P +RLGSGP+   +IK HKWF+ +NWKKLE RE+QP FKP V G  C ANFD CWT M  
Sbjct: 388 PERRLGSGPSGAEEIKKHKWFKAINWKKLEAREVQPSFKPAVSGRQCIANFDKCWTDMSV 447

Query: 461 DDSPAPTPTD---GGHFHGYSYVAP 479
            DSPA +P        F  ++YV P
Sbjct: 448 LDSPASSPNSDAKANPFTNFTYVRP 459

BLAST of Carg18160 vs. ExPASy Swiss-Prot
Match: P42818 (Serine/threonine-protein kinase AtPK1/AtPK6 OS=Arabidopsis thaliana OX=3702 GN=ATPK1 PE=1 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 3.8e-138
Identity = 255/451 (56.54%), Postives = 327/451 (72.51%), Query Frame = 0

Query: 41  PPSSSTAALLDFDFSDVFGPTNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPS 100
           P +S     ++ +FSDVFGP    LP    D  ++E  PAV+++RSHS VGP        
Sbjct: 22  PSNSVLKDDVELEFSDVFGP----LPEEANDIAYDE--PAVVYSRSHSLVGPCS--LDSH 81

Query: 101 SLSFFREVKSQIEEEEDDLEISTGDGVQKE---KGADESENDGGENSE-----GKVGVGD 160
           SL   +    + E+  D +E   G+ +++     G D+S+N+     +     G VG+ D
Sbjct: 82  SLKLTKLTLLETEDSIDLVECLEGESLKENDDFSGNDDSDNEKALEGDLVKVSGVVGIDD 141

Query: 161 FDILRVVGKGAFGKVFLVRKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDYMKAERDI 220
           F++++VVGKGAFGKV+ VRKK  S+        IYAMKVMRKD I++KNH +YMKAERDI
Sbjct: 142 FEVMKVVGKGAFGKVYQVRKKETSE--------IYAMKVMRKDHIMEKNHAEYMKAERDI 201

Query: 221 LTKVSHPFIVQLRYSFQSKSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYAAEIVSAV 280
           LTK+ HPFIVQL+YSFQ+K +LYL+LDFINGGHLFF LY QG+F ED ARVY AEIVSAV
Sbjct: 202 LTKIDHPFIVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHQGLFREDLARVYTAEIVSAV 261

Query: 281 SHLHDCGIVHRDLKPENILMDADGHVVLTDFGLAKEIDESSRSNSLCGTTEYMAPEILLS 340
           SHLH+ GI+HRDLKPENILMD DGHV+LTDFGLAKE +E++RSNS+CGTTEYMAPEI+  
Sbjct: 262 SHLHEKGIMHRDLKPENILMDTDGHVMLTDFGLAKEFEENTRSNSMCGTTEYMAPEIVRG 321

Query: 341 KGHNKDADWWSVGVLLYEMLSGKPPFTHANRKKLQERIIKEKVKLPSYLSTEAHSLLRGL 400
           KGH+K ADWWSVG+LLYEML+GKPPF   ++ K+Q++I+K+K+KLP +LS EAH++L+GL
Sbjct: 322 KGHDKAADWWSVGILLYEMLTGKPPFL-GSKGKIQQKIVKDKIKLPQFLSNEAHAILKGL 381

Query: 401 LQKDPSKRLGSGPNRGADIKNHKWFRPVNWKKLECRELQPKFKPDVKGNDCTANFDNCWT 460
           LQK+P +RLGSG +   +IK HKWF+ +NWKKLE RE+ P FKP+V G  C ANFD CWT
Sbjct: 382 LQKEPERRLGSGLSGAEEIKQHKWFKGINWKKLEAREVMPSFKPEVSGRQCIANFDKCWT 441

Query: 461 TMPPDDSPAPTPTD---GGHFHGYSYVAPDP 481
            M   DSPA +P+       F  ++YV P P
Sbjct: 442 DMSVLDSPASSPSSDPKANPFTNFTYVRPPP 455

BLAST of Carg18160 vs. ExPASy Swiss-Prot
Match: P18652 (Ribosomal protein S6 kinase 2 alpha OS=Gallus gallus OX=9031 GN=RPS6KA PE=2 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.9e-89
Identity = 177/376 (47.07%), Postives = 248/376 (65.96%), Query Frame = 0

Query: 107 EVKSQIEEEEDDLEIST--GDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAF 166
           + KS I   E DL  ST  G+GV KE        +G E    K     F++L+V+G+G+F
Sbjct: 36  KAKSDITWVEKDLVDSTDKGEGVVKEINITHHVKEGSE----KADPSQFELLKVLGQGSF 95

Query: 167 GKVFLVRKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQL 226
           GKVFLVRK        P+++ +YAMKV++K T+  ++ V   K ERDIL  V+HPF+V+L
Sbjct: 96  GKVFLVRKI-----TPPDSNHLYAMKVLKKATLKVRDRV-RTKIERDILADVNHPFVVKL 155

Query: 227 RYSFQSKSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRD 286
            Y+FQ++ KLYLILDF+ GG LF  L ++ +F+E+  + Y AE+   + HLH  GI++RD
Sbjct: 156 HYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRD 215

Query: 287 LKPENILMDADGHVVLTDFGLAKE-IDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWS 346
           LKPENIL+D +GH+ LTDFGL+KE ID   ++ S CGT EYMAPE++  +GH+  ADWWS
Sbjct: 216 LKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWS 275

Query: 347 VGVLLYEMLSGKPPFTHANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGS 406
            GVL++EML+G  PF   +RK+    I+K K+ +P +LS EA SLLR L +++P+ RLGS
Sbjct: 276 YGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSAEAQSLLRALFKRNPANRLGS 335

Query: 407 GPNRGADIKNHKWFRPVNWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPT 466
           GP+   +IK H ++  ++W KL  RE++P FKP V   D T  FD  +T+  P DSP   
Sbjct: 336 GPDGAEEIKRHPFYSTIDWNKLYRREIKPPFKPAVGQPDDTFYFDTEFTSRTPKDSPGIP 395

Query: 467 PTDGGH--FHGYSYVA 478
           P+ G H  F G+S+VA
Sbjct: 396 PSAGAHQLFRGFSFVA 401

BLAST of Carg18160 vs. ExPASy Swiss-Prot
Match: Q63531 (Ribosomal protein S6 kinase alpha-1 OS=Rattus norvegicus OX=10116 GN=Rps6ka1 PE=1 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 7.2e-89
Identity = 174/370 (47.03%), Postives = 242/370 (65.41%), Query Frame = 0

Query: 111 QIEEEEDDLEISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLV 170
           Q   EE  L+ S  +G+ KE         G E    K     F++L+V+G+G+FGKVFLV
Sbjct: 24  QASGEEAGLQPSKDEGILKEISITHHVKAGSE----KADPSHFELLKVLGQGSFGKVFLV 83

Query: 171 RKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQS 230
           RK        P+N  +YAMKV++K T+  ++ V   K ERDIL  V+HPF+V+L Y+FQ+
Sbjct: 84  RKV-----TRPDNGHLYAMKVLKKATLKVRDRV-RTKMERDILADVNHPFVVKLHYAFQT 143

Query: 231 KSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENI 290
           + KLYLILDF+ GG LF  L ++ +F+E+  + Y AE+   + HLH  GI++RDLKPENI
Sbjct: 144 EGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENI 203

Query: 291 LMDADGHVVLTDFGLAKE-IDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLY 350
           L+D +GH+ LTDFGL+KE ID   ++ S CGT EYMAPE++  +GH   ADWWS GVL++
Sbjct: 204 LLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHTHSADWWSYGVLMF 263

Query: 351 EMLSGKPPFTHANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGA 410
           EML+G  PF   +RK+    I+K K+ +P +LSTEA SLLR L +++P+ RLGSGP+   
Sbjct: 264 EMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAE 323

Query: 411 DIKNHKWFRPVNWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGH 470
           +IK H ++  ++W KL  RE++P FKP V   D T  FD  +T+  P DSP   P+ G H
Sbjct: 324 EIKRHIFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPRDSPGIPPSAGAH 383

Query: 471 --FHGYSYVA 478
             F G+S+VA
Sbjct: 384 QLFRGFSFVA 383

BLAST of Carg18160 vs. ExPASy Swiss-Prot
Match: Q15418 (Ribosomal protein S6 kinase alpha-1 OS=Homo sapiens OX=9606 GN=RPS6KA1 PE=1 SV=2)

HSP 1 Score: 328.9 bits (842), Expect = 9.4e-89
Identity = 174/370 (47.03%), Postives = 243/370 (65.68%), Query Frame = 0

Query: 111 QIEEEEDDLEISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLV 170
           Q   EE  L+ S  +GV KE         G E    K     F++L+V+G+G+FGKVFLV
Sbjct: 24  QTSGEEAGLQPSKDEGVLKEISITHHVKAGSE----KADPSHFELLKVLGQGSFGKVFLV 83

Query: 171 RKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQS 230
           RK        P++  +YAMKV++K T+  ++ V   K ERDIL  V+HPF+V+L Y+FQ+
Sbjct: 84  RKV-----TRPDSGHLYAMKVLKKATLKVRDRV-RTKMERDILADVNHPFVVKLHYAFQT 143

Query: 231 KSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENI 290
           + KLYLILDF+ GG LF  L ++ +F+E+  + Y AE+   + HLH  GI++RDLKPENI
Sbjct: 144 EGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENI 203

Query: 291 LMDADGHVVLTDFGLAKE-IDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLY 350
           L+D +GH+ LTDFGL+KE ID   ++ S CGT EYMAPE++  +GH+  ADWWS GVL++
Sbjct: 204 LLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMF 263

Query: 351 EMLSGKPPFTHANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGA 410
           EML+G  PF   +RK+    I+K K+ +P +LSTEA SLLR L +++P+ RLGSGP+   
Sbjct: 264 EMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAE 323

Query: 411 DIKNHKWFRPVNWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGH 470
           +IK H ++  ++W KL  RE++P FKP V   D T  FD  +T+  P DSP   P+ G H
Sbjct: 324 EIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAH 383

Query: 471 --FHGYSYVA 478
             F G+S+VA
Sbjct: 384 QLFRGFSFVA 383

BLAST of Carg18160 vs. ExPASy TrEMBL
Match: A0A6J1EZG5 (serine/threonine-protein kinase AtPK1/AtPK6-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111440587 PE=3 SV=1)

HSP 1 Score: 974.2 bits (2517), Expect = 2.1e-280
Identity = 484/486 (99.59%), Postives = 484/486 (99.59%), Query Frame = 0

Query: 1   MVYASTHNKNMHALLTAKLTKLTLPSSSSSSPPPPPPPPPPPSSSTAALLDFDFSDVFGP 60
           MVYASTHNKNMHALLTAKLTKLTLP SSSSSPPPPPPPPPP SSSTAALLDFDFSDVFGP
Sbjct: 1   MVYASTHNKNMHALLTAKLTKLTLP-SSSSSPPPPPPPPPPSSSSTAALLDFDFSDVFGP 60

Query: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120
           TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE
Sbjct: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120

Query: 121 ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180
           ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG
Sbjct: 121 ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180

Query: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240
           PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF
Sbjct: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240

Query: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL 300
           INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL
Sbjct: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL 300

Query: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360
           TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH
Sbjct: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360

Query: 361 ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420
           ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV
Sbjct: 361 ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420

Query: 421 NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP 480
           NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP
Sbjct: 421 NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP 480

Query: 481 WLSSSG 487
           WLSSSG
Sbjct: 481 WLSSSG 485

BLAST of Carg18160 vs. ExPASy TrEMBL
Match: A0A6J1I988 (serine/threonine-protein kinase AtPK2/AtPK19-like OS=Cucurbita maxima OX=3661 GN=LOC111471181 PE=3 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 1.4e-276
Identity = 479/486 (98.56%), Postives = 480/486 (98.77%), Query Frame = 0

Query: 1   MVYASTHNKNMHALLTAKLTKLTLPSSSSSSPPPPPPPPPPPSSSTAALLDFDFSDVFGP 60
           MVYASTHNKNMHALLTAKLTKLTLPSSSSS   PPPPPPPPPSSSTAALLDFDFSDVFGP
Sbjct: 1   MVYASTHNKNMHALLTAKLTKLTLPSSSSS---PPPPPPPPPSSSTAALLDFDFSDVFGP 60

Query: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120
           TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE
Sbjct: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLE 120

Query: 121 ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180
           ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG
Sbjct: 121 ISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNG 180

Query: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240
           PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF
Sbjct: 181 PNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDF 240

Query: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL 300
           INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL
Sbjct: 241 INGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVL 300

Query: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360
           TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH
Sbjct: 301 TDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTH 360

Query: 361 ANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420
           ANRKKLQERIIKEKVKLP YLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV
Sbjct: 361 ANRKKLQERIIKEKVKLPPYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPV 420

Query: 421 NWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDP 480
           NWKKLE RELQPKFKPDVKGNDCTANFDNCWTTM PDDSPAPTPT+GGHFHGYSYVAPDP
Sbjct: 421 NWKKLEYRELQPKFKPDVKGNDCTANFDNCWTTMAPDDSPAPTPTNGGHFHGYSYVAPDP 480

Query: 481 WLSSSG 487
           WLSSSG
Sbjct: 481 WLSSSG 483

BLAST of Carg18160 vs. ExPASy TrEMBL
Match: A0A6J1EZG6 (serine/threonine-protein kinase AtPK1/AtPK6-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111440587 PE=4 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 5.3e-268
Identity = 460/461 (99.78%), Postives = 460/461 (99.78%), Query Frame = 0

Query: 26  SSSSSSPPPPPPPPPPPSSSTAALLDFDFSDVFGPTNSSLPSFPGDPQHNEQQPAVIHNR 85
           SSSSSSPPPPPPPPPP SSSTAALLDFDFSDVFGPTNSSLPSFPGDPQHNEQQPAVIHNR
Sbjct: 47  SSSSSSPPPPPPPPPPSSSSTAALLDFDFSDVFGPTNSSLPSFPGDPQHNEQQPAVIHNR 106

Query: 86  SHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLEISTGDGVQKEKGADESENDGGENSE 145
           SHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLEISTGDGVQKEKGADESENDGGENSE
Sbjct: 107 SHSFVGPSPRLTPPSSLSFFREVKSQIEEEEDDLEISTGDGVQKEKGADESENDGGENSE 166

Query: 146 GKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDY 205
           GKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDY
Sbjct: 167 GKVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDY 226

Query: 206 MKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYA 265
           MKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYA
Sbjct: 227 MKAERDILTKVSHPFIVQLRYSFQSKSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYA 286

Query: 266 AEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVLTDFGLAKEIDESSRSNSLCGTTEYM 325
           AEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVLTDFGLAKEIDESSRSNSLCGTTEYM
Sbjct: 287 AEIVSAVSHLHDCGIVHRDLKPENILMDADGHVVLTDFGLAKEIDESSRSNSLCGTTEYM 346

Query: 326 APEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTHANRKKLQERIIKEKVKLPSYLSTEA 385
           APEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTHANRKKLQERIIKEKVKLPSYLSTEA
Sbjct: 347 APEILLSKGHNKDADWWSVGVLLYEMLSGKPPFTHANRKKLQERIIKEKVKLPSYLSTEA 406

Query: 386 HSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPVNWKKLECRELQPKFKPDVKGNDCTA 445
           HSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPVNWKKLECRELQPKFKPDVKGNDCTA
Sbjct: 407 HSLLRGLLQKDPSKRLGSGPNRGADIKNHKWFRPVNWKKLECRELQPKFKPDVKGNDCTA 466

Query: 446 NFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDPWLSSSG 487
           NFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDPWLSSSG
Sbjct: 467 NFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVAPDPWLSSSG 507

BLAST of Carg18160 vs. ExPASy TrEMBL
Match: A0A6J1E180 (serine/threonine-protein kinase AtPK2/AtPK19-like OS=Cucurbita moschata OX=3662 GN=LOC111429871 PE=3 SV=1)

HSP 1 Score: 835.5 bits (2157), Expect = 1.1e-238
Identity = 421/489 (86.09%), Postives = 444/489 (90.80%), Query Frame = 0

Query: 1   MVYASTHNKNMHALLTAKLTKLTLPSSSSSSPPPPPPPPPPPSSSTAALLDFDFSDVFGP 60
           MV AS  +KNMH+LL A LTKLT+PSSS SS PPPP       SSTA   DFDFSDVFGP
Sbjct: 1   MVSASNRHKNMHSLLVASLTKLTIPSSSPSSSPPPP-------SSTA---DFDFSDVFGP 60

Query: 61  TNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREV--KSQIEEEEDD 120
           TN S   FP DPQH+EQQP VIHNRSHSFVGPSPRLTPPSSL FFREV  +S+ E+EEDD
Sbjct: 61  TNPSSSCFPTDPQHHEQQPEVIHNRSHSFVGPSPRLTPPSSLPFFREVDPQSEEEDEEDD 120

Query: 121 LEISTGDGVQKE-KGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGDSK 180
           LEIST +G Q+E KGADESE+ GGE ++GK+GV DFDILRVVGKGAFGKVFLVRKKGD K
Sbjct: 121 LEISTQNGAQEEMKGADESESGGGEKTQGKIGVADFDILRVVGKGAFGKVFLVRKKGDCK 180

Query: 181 GNGPNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLYLI 240
           GNG NNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKV+HPFIVQLRYSFQ+KSKLYLI
Sbjct: 181 GNGSNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVAHPFIVQLRYSFQTKSKLYLI 240

Query: 241 LDFINGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDADGH 300
           LDFINGGHLFFHLYRQGIFSEDQAR+YAAEIVSAVSHLH CGIVHRDLKPENILMDADGH
Sbjct: 241 LDFINGGHLFFHLYRQGIFSEDQARIYAAEIVSAVSHLHSCGIVHRDLKPENILMDADGH 300

Query: 301 VVLTDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPP 360
           VVLTDFGLAKEI+ SSRSNS+CGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSG+PP
Sbjct: 301 VVLTDFGLAKEIEISSRSNSMCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGQPP 360

Query: 361 FTHANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHKWF 420
           FTHANRKKLQERIIKEKVKLP YLSTEAHSLLRGLLQK+PS RLGSGPN G +IK HKWF
Sbjct: 361 FTHANRKKLQERIIKEKVKLPPYLSTEAHSLLRGLLQKEPSTRLGSGPNGGDEIKKHKWF 420

Query: 421 RPVNWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSYVA 480
           RPVNWKKLECRELQPKFKPDV GNDCTANFD+CWT MPPDDSPAPTPTDGGHF GYSYVA
Sbjct: 421 RPVNWKKLECRELQPKFKPDVNGNDCTANFDHCWTAMPPDDSPAPTPTDGGHFQGYSYVA 479

Query: 481 PDPWLSSSG 487
           P+PWLSSSG
Sbjct: 481 PNPWLSSSG 479

BLAST of Carg18160 vs. ExPASy TrEMBL
Match: A0A6J1BZL8 (serine/threonine-protein kinase AtPK1/AtPK6-like OS=Momordica charantia OX=3673 GN=LOC111007153 PE=3 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 1.3e-237
Identity = 419/491 (85.34%), Postives = 443/491 (90.22%), Query Frame = 0

Query: 1   MVYASTHNKNMHALLTAKLTKLTLPSSSSSSPPPPPPPPPPPSSSTAALLDFDFSDVFGP 60
           MV AS  NK MH+LL A L KLT+PSSSSS        P P +++ AA+ DFDFSDVFGP
Sbjct: 1   MVSASARNKTMHSLLAANLAKLTIPSSSSS--------PSPSTAAAAAIHDFDFSDVFGP 60

Query: 61  TN---SSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPSSLSFFREVKSQIEEEED 120
           TN   SS  SFPGDPQH+E QP VIHNRSHSFVGPSPRLTPPSSL FF+ + SQ +EEED
Sbjct: 61  TNPSPSSSTSFPGDPQHHE-QPEVIHNRSHSFVGPSPRLTPPSSLPFFQGIDSQSDEEED 120

Query: 121 --DLEISTGDGVQKEKGADESENDGGENSEGKVGVGDFDILRVVGKGAFGKVFLVRKKGD 180
             D EIST +G Q+EKG  ES N GGEN +GK+GVGDFDILRVVGKGAFGKVFLVRKKGD
Sbjct: 121 EEDSEISTSNGAQEEKGVGESVNGGGENVQGKIGVGDFDILRVVGKGAFGKVFLVRKKGD 180

Query: 181 SKGNGPNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVSHPFIVQLRYSFQSKSKLY 240
            KGNGPNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKV+HPFIVQLRYSFQ+KSKLY
Sbjct: 181 LKGNGPNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKVAHPFIVQLRYSFQTKSKLY 240

Query: 241 LILDFINGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLHDCGIVHRDLKPENILMDAD 300
           LILDFINGGHLF+HLYRQGIFSEDQARVYAAEIVSAVSHLH CGIVHRDLKPENILMDAD
Sbjct: 241 LILDFINGGHLFYHLYRQGIFSEDQARVYAAEIVSAVSHLHGCGIVHRDLKPENILMDAD 300

Query: 301 GHVVLTDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGK 360
           GHVVLTDFGLAKEI+ESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSG+
Sbjct: 301 GHVVLTDFGLAKEIEESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGQ 360

Query: 361 PPFTHANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHK 420
           PPFTHANRKKLQERIIKEKVKLP  LSTEAHSLLRGLLQK+PSKRLGSGPN G +IK HK
Sbjct: 361 PPFTHANRKKLQERIIKEKVKLPPCLSTEAHSLLRGLLQKEPSKRLGSGPNGGDEIKKHK 420

Query: 421 WFRPVNWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPPDDSPAPTPTDGGHFHGYSY 480
           WFRPVNWKKLECRELQPKFKPDV GNDCTANFD+CWTTMPPDDSPAPTPTDGGHFHGY+Y
Sbjct: 421 WFRPVNWKKLECRELQPKFKPDVNGNDCTANFDHCWTTMPPDDSPAPTPTDGGHFHGYTY 480

Query: 481 VAPDPWLSSSG 487
           VAP+PWLSSSG
Sbjct: 481 VAPNPWLSSSG 482

BLAST of Carg18160 vs. TAIR 10
Match: AT3G08720.1 (serine/threonine protein kinase 2 )

HSP 1 Score: 503.8 bits (1296), Expect = 1.5e-142
Identity = 258/445 (57.98%), Postives = 326/445 (73.26%), Query Frame = 0

Query: 41  PPSSSTAALLDFDFSDVFGPTNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPS 100
           PP S     L+  FSDVFGP   +      D  ++E  PAV+++RSHS VGPS  L    
Sbjct: 28  PPKSVLGDNLELQFSDVFGPMPEANSEEACDVAYDE--PAVVYSRSHSLVGPS--LVVSH 87

Query: 101 SLSFFREVKSQIEEEEDDLEISTGDGVQKE---KGADESEND-GGENSEGKVGVGDFDIL 160
           SL   +    + E+  D +E   G+ +++     G D+++++   E   G VG+ DF++L
Sbjct: 88  SLKMNKLTLRETEDSVDLVECVEGESIKENDEFSGNDDTDSEKSPEEVSGVVGIEDFEVL 147

Query: 161 RVVGKGAFGKVFLVRKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKV 220
           +VVG+GAFGKV+ VRKK  S+        IYAMKVMRKD I++KNH +YMKAERDILTK+
Sbjct: 148 KVVGQGAFGKVYQVRKKDTSE--------IYAMKVMRKDKIVEKNHAEYMKAERDILTKI 207

Query: 221 SHPFIVQLRYSFQSKSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLH 280
            HPFIVQL+YSFQ+K +LYL+LDFINGGHLFF LY QG+F ED ARVY AEIVSAVSHLH
Sbjct: 208 DHPFIVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHQGLFREDLARVYTAEIVSAVSHLH 267

Query: 281 DCGIVHRDLKPENILMDADGHVVLTDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHN 340
           + GI+HRDLKPENILMD DGHV+LTDFGLAKE +E++RSNS+CGTTEYMAPEI+  KGH+
Sbjct: 268 EKGIMHRDLKPENILMDVDGHVMLTDFGLAKEFEENTRSNSMCGTTEYMAPEIVRGKGHD 327

Query: 341 KDADWWSVGVLLYEMLSGKPPFTHANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKD 400
           K ADWWSVG+LLYEML+GKPPF   ++ K+Q++I+K+K+KLP +LS EAH+LL+GLLQK+
Sbjct: 328 KAADWWSVGILLYEMLTGKPPFL-GSKGKIQQKIVKDKIKLPQFLSNEAHALLKGLLQKE 387

Query: 401 PSKRLGSGPNRGADIKNHKWFRPVNWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPP 460
           P +RLGSGP+   +IK HKWF+ +NWKKLE RE+QP FKP V G  C ANFD CWT M  
Sbjct: 388 PERRLGSGPSGAEEIKKHKWFKAINWKKLEAREVQPSFKPAVSGRQCIANFDKCWTDMSV 447

Query: 461 DDSPAPTPTD---GGHFHGYSYVAP 479
            DSPA +P        F  ++YV P
Sbjct: 448 LDSPASSPNSDAKANPFTNFTYVRP 459

BLAST of Carg18160 vs. TAIR 10
Match: AT3G08720.2 (serine/threonine protein kinase 2 )

HSP 1 Score: 503.8 bits (1296), Expect = 1.5e-142
Identity = 258/445 (57.98%), Postives = 326/445 (73.26%), Query Frame = 0

Query: 41  PPSSSTAALLDFDFSDVFGPTNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPS 100
           PP S     L+  FSDVFGP   +      D  ++E  PAV+++RSHS VGPS  L    
Sbjct: 28  PPKSVLGDNLELQFSDVFGPMPEANSEEACDVAYDE--PAVVYSRSHSLVGPS--LVVSH 87

Query: 101 SLSFFREVKSQIEEEEDDLEISTGDGVQKE---KGADESEND-GGENSEGKVGVGDFDIL 160
           SL   +    + E+  D +E   G+ +++     G D+++++   E   G VG+ DF++L
Sbjct: 88  SLKMNKLTLRETEDSVDLVECVEGESIKENDEFSGNDDTDSEKSPEEVSGVVGIEDFEVL 147

Query: 161 RVVGKGAFGKVFLVRKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDYMKAERDILTKV 220
           +VVG+GAFGKV+ VRKK  S+        IYAMKVMRKD I++KNH +YMKAERDILTK+
Sbjct: 148 KVVGQGAFGKVYQVRKKDTSE--------IYAMKVMRKDKIVEKNHAEYMKAERDILTKI 207

Query: 221 SHPFIVQLRYSFQSKSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYAAEIVSAVSHLH 280
            HPFIVQL+YSFQ+K +LYL+LDFINGGHLFF LY QG+F ED ARVY AEIVSAVSHLH
Sbjct: 208 DHPFIVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHQGLFREDLARVYTAEIVSAVSHLH 267

Query: 281 DCGIVHRDLKPENILMDADGHVVLTDFGLAKEIDESSRSNSLCGTTEYMAPEILLSKGHN 340
           + GI+HRDLKPENILMD DGHV+LTDFGLAKE +E++RSNS+CGTTEYMAPEI+  KGH+
Sbjct: 268 EKGIMHRDLKPENILMDVDGHVMLTDFGLAKEFEENTRSNSMCGTTEYMAPEIVRGKGHD 327

Query: 341 KDADWWSVGVLLYEMLSGKPPFTHANRKKLQERIIKEKVKLPSYLSTEAHSLLRGLLQKD 400
           K ADWWSVG+LLYEML+GKPPF   ++ K+Q++I+K+K+KLP +LS EAH+LL+GLLQK+
Sbjct: 328 KAADWWSVGILLYEMLTGKPPFL-GSKGKIQQKIVKDKIKLPQFLSNEAHALLKGLLQKE 387

Query: 401 PSKRLGSGPNRGADIKNHKWFRPVNWKKLECRELQPKFKPDVKGNDCTANFDNCWTTMPP 460
           P +RLGSGP+   +IK HKWF+ +NWKKLE RE+QP FKP V G  C ANFD CWT M  
Sbjct: 388 PERRLGSGPSGAEEIKKHKWFKAINWKKLEAREVQPSFKPAVSGRQCIANFDKCWTDMSV 447

Query: 461 DDSPAPTPTD---GGHFHGYSYVAP 479
            DSPA +P        F  ++YV P
Sbjct: 448 LDSPASSPNSDAKANPFTNFTYVRP 459

BLAST of Carg18160 vs. TAIR 10
Match: AT3G08730.1 (protein-serine kinase 1 )

HSP 1 Score: 493.0 bits (1268), Expect = 2.7e-139
Identity = 255/451 (56.54%), Postives = 327/451 (72.51%), Query Frame = 0

Query: 41  PPSSSTAALLDFDFSDVFGPTNSSLPSFPGDPQHNEQQPAVIHNRSHSFVGPSPRLTPPS 100
           P +S     ++ +FSDVFGP    LP    D  ++E  PAV+++RSHS VGP        
Sbjct: 22  PSNSVLKDDVELEFSDVFGP----LPEEANDIAYDE--PAVVYSRSHSLVGPCS--LDSH 81

Query: 101 SLSFFREVKSQIEEEEDDLEISTGDGVQKE---KGADESENDGGENSE-----GKVGVGD 160
           SL   +    + E+  D +E   G+ +++     G D+S+N+     +     G VG+ D
Sbjct: 82  SLKLTKLTLLETEDSIDLVECLEGESLKENDDFSGNDDSDNEKALEGDLVKVSGVVGIDD 141

Query: 161 FDILRVVGKGAFGKVFLVRKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDYMKAERDI 220
           F++++VVGKGAFGKV+ VRKK  S+        IYAMKVMRKD I++KNH +YMKAERDI
Sbjct: 142 FEVMKVVGKGAFGKVYQVRKKETSE--------IYAMKVMRKDHIMEKNHAEYMKAERDI 201

Query: 221 LTKVSHPFIVQLRYSFQSKSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYAAEIVSAV 280
           LTK+ HPFIVQL+YSFQ+K +LYL+LDFINGGHLFF LY QG+F ED ARVY AEIVSAV
Sbjct: 202 LTKIDHPFIVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHQGLFREDLARVYTAEIVSAV 261

Query: 281 SHLHDCGIVHRDLKPENILMDADGHVVLTDFGLAKEIDESSRSNSLCGTTEYMAPEILLS 340
           SHLH+ GI+HRDLKPENILMD DGHV+LTDFGLAKE +E++RSNS+CGTTEYMAPEI+  
Sbjct: 262 SHLHEKGIMHRDLKPENILMDTDGHVMLTDFGLAKEFEENTRSNSMCGTTEYMAPEIVRG 321

Query: 341 KGHNKDADWWSVGVLLYEMLSGKPPFTHANRKKLQERIIKEKVKLPSYLSTEAHSLLRGL 400
           KGH+K ADWWSVG+LLYEML+GKPPF   ++ K+Q++I+K+K+KLP +LS EAH++L+GL
Sbjct: 322 KGHDKAADWWSVGILLYEMLTGKPPFL-GSKGKIQQKIVKDKIKLPQFLSNEAHAILKGL 381

Query: 401 LQKDPSKRLGSGPNRGADIKNHKWFRPVNWKKLECRELQPKFKPDVKGNDCTANFDNCWT 460
           LQK+P +RLGSG +   +IK HKWF+ +NWKKLE RE+ P FKP+V G  C ANFD CWT
Sbjct: 382 LQKEPERRLGSGLSGAEEIKQHKWFKGINWKKLEAREVMPSFKPEVSGRQCIANFDKCWT 441

Query: 461 TMPPDDSPAPTPTD---GGHFHGYSYVAPDP 481
            M   DSPA +P+       F  ++YV P P
Sbjct: 442 DMSVLDSPASSPSSDPKANPFTNFTYVRPPP 455

BLAST of Carg18160 vs. TAIR 10
Match: AT5G62310.1 (AGC (cAMP-dependent, cGMP-dependent and protein kinase C) kinase family protein )

HSP 1 Score: 232.6 bits (592), Expect = 6.5e-61
Identity = 136/406 (33.50%), Postives = 226/406 (55.67%), Query Frame = 0

Query: 103  SFFREVKSQIEEEE-------DDLEISTGDGV-QKEKGADES------ENDGGENSEGKV 162
            +F R ++  ++E+        DD ++ + + +  +E  ADE        +     ++ + 
Sbjct: 690  TFGRRIEKLLQEKYIELCGLIDDEKVDSSNAMPDEESSADEDTVRSLRASPLNPRAKDRT 749

Query: 163  GVGDFDILRVVGKGAFGKVFLVRKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDYMKA 222
             + DF+I++ + +GAFG+VFL +K+            ++A+KV++K  +I+KN V+ + A
Sbjct: 750  SIEDFEIIKPISRGAFGRVFLAKKRA--------TGDLFAIKVLKKADMIRKNAVESILA 809

Query: 223  ERDILTKVSHPFIVQLRYSFQSKSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYAAEI 282
            ER+IL  V +PF+V+  YSF  +  LYL+++++NGG LF  L   G   ED AR+Y AE+
Sbjct: 810  ERNILISVRNPFVVRFFYSFTCRENLYLVMEYLNGGDLFSLLRNLGCLDEDMARIYIAEV 869

Query: 283  VSAVSHLHDCGIVHRDLKPENILMDADGHVVLTDFGLAK--------------------- 342
            V A+ +LH   I+HRDLKP+N+L++ DGH+ LTDFGL+K                     
Sbjct: 870  VLALEYLHSVNIIHRDLKPDNLLINQDGHIKLTDFGLSKVGLINSTDDLSGESSLGNSGF 929

Query: 343  -----------EIDESSRSNSLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKP 402
                       +  +S + +++ GT +Y+APEILL  GH K ADWWSVGV+L+E+L G P
Sbjct: 930  FAEDGSKAQHSQGKDSRKKHAVVGTPDYLAPEILLGMGHGKTADWWSVGVILFEVLVGIP 989

Query: 403  PFTHANRKKLQERIIKEKV---KLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKN 460
            PF     +++ E II   +    +P  +S EAH L+  LL ++P +RLG+      ++K 
Sbjct: 990  PFNAETPQQIFENIINRDIPWPNVPEEISYEAHDLINKLLTENPVQRLGA--TGAGEVKQ 1049

BLAST of Carg18160 vs. TAIR 10
Match: AT1G45160.1 (Protein kinase superfamily protein )

HSP 1 Score: 231.5 bits (589), Expect = 1.5e-60
Identity = 122/310 (39.35%), Postives = 189/310 (60.97%), Query Frame = 0

Query: 147 KVGVGDFDILRVVGKGAFGKVFLVRKKGDSKGNGPNNDGIYAMKVMRKDTIIKKNHVDYM 206
           ++ + DF+I++ + +GAFGKVFL RK+             +A+KV++K  +I+KN ++ +
Sbjct: 664 RISIDDFEIIKPISRGAFGKVFLARKR--------TTGDFFAIKVLKKLDMIRKNDIERI 723

Query: 207 KAERDILTKVSHPFIVQLRYSFQSKSKLYLILDFINGGHLFFHLYRQGIFSEDQARVYAA 266
             ER+IL  V +PF+V+  YSF  +  LYL+++++NGG L+  L + G   E+ AR+Y A
Sbjct: 724 LQERNILITVRYPFLVRFFYSFTCRDNLYLVMEYLNGGDLYSLLQKVGCLDEEIARIYIA 783

Query: 267 EIVSAVSHLHDCGIVHRDLKPENILMDADGHVVLTDFGLAK-------------EIDESS 326
           E+V A+ +LH   IVHRDLKP+N+L+  +GH+ LTDFGL+K             E D S 
Sbjct: 784 ELVLALEYLHSLKIVHRDLKPDNLLIAYNGHIKLTDFGLSKIGLINNTIDLSGHESDVSP 843

Query: 327 RSN---------------SLCGTTEYMAPEILLSKGHNKDADWWSVGVLLYEMLSGKPPF 386
           R+N               S  GT +Y+APEILL   H   ADWWS G++L+E+L+G PPF
Sbjct: 844 RTNSHHFQKNQEEERIRHSAVGTPDYLAPEILLGTEHGYAADWWSAGIVLFELLTGIPPF 903

Query: 387 THANRKKLQERIIKEKV---KLPSYLSTEAHSLLRGLLQKDPSKRLGSGPNRGADIKNHK 426
           T +  +K+ + I+  K+    +P  +S EA  L+  LL  +P KRLG+  N  A++K+H 
Sbjct: 904 TASRPEKIFDNILNGKMPWPDVPGEMSYEAQDLINRLLVHEPEKRLGA--NGAAEVKSHP 963

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021487.19.1e-283100.00Serine/threonine-protein kinase AtPK2/AtPK19, partial [Cucurbita argyrosperma su... [more]
KAG6587502.16.6e-28199.59Serine/threonine-protein kinase AtPK2/AtPK19, partial [Cucurbita argyrosperma su... [more]
XP_022933334.14.2e-28099.59serine/threonine-protein kinase AtPK1/AtPK6-like isoform X2 [Cucurbita moschata][more]
XP_022972650.12.8e-27698.56serine/threonine-protein kinase AtPK2/AtPK19-like [Cucurbita maxima][more]
XP_023531988.11.1e-27598.56serine/threonine-protein kinase AtPK2/AtPK19-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q390302.1e-14157.98Serine/threonine-protein kinase AtPK2/AtPK19 OS=Arabidopsis thaliana OX=3702 GN=... [more]
P428183.8e-13856.54Serine/threonine-protein kinase AtPK1/AtPK6 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
P186521.9e-8947.07Ribosomal protein S6 kinase 2 alpha OS=Gallus gallus OX=9031 GN=RPS6KA PE=2 SV=1[more]
Q635317.2e-8947.03Ribosomal protein S6 kinase alpha-1 OS=Rattus norvegicus OX=10116 GN=Rps6ka1 PE=... [more]
Q154189.4e-8947.03Ribosomal protein S6 kinase alpha-1 OS=Homo sapiens OX=9606 GN=RPS6KA1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1EZG52.1e-28099.59serine/threonine-protein kinase AtPK1/AtPK6-like isoform X2 OS=Cucurbita moschat... [more]
A0A6J1I9881.4e-27698.56serine/threonine-protein kinase AtPK2/AtPK19-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1EZG65.3e-26899.78serine/threonine-protein kinase AtPK1/AtPK6-like isoform X1 OS=Cucurbita moschat... [more]
A0A6J1E1801.1e-23886.09serine/threonine-protein kinase AtPK2/AtPK19-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1BZL81.3e-23785.34serine/threonine-protein kinase AtPK1/AtPK6-like OS=Momordica charantia OX=3673 ... [more]
Match NameE-valueIdentityDescription
AT3G08720.11.5e-14257.98serine/threonine protein kinase 2 [more]
AT3G08720.21.5e-14257.98serine/threonine protein kinase 2 [more]
AT3G08730.12.7e-13956.54protein-serine kinase 1 [more]
AT5G62310.16.5e-6133.50AGC (cAMP-dependent, cGMP-dependent and protein kinase C) kinase family protein [more]
AT1G45160.11.5e-6039.35Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 153..417
e-value: 1.3E-105
score: 366.8
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 154..417
e-value: 2.3E-72
score: 243.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 153..417
score: 52.795448
IPR000961AGC-kinase, C-terminalSMARTSM00133pkinase_C_6coord: 418..477
e-value: 8.1E-12
score: 55.3
IPR000961AGC-kinase, C-terminalPROSITEPS51285AGC_KINASE_CTERcoord: 418..485
score: 13.239367
IPR017892Protein kinase, C-terminalPFAMPF00433Pkinase_Ccoord: 438..475
e-value: 1.8E-4
score: 22.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 152..459
e-value: 7.9E-127
score: 424.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 241..436
e-value: 7.9E-127
score: 424.6
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 143..418
e-value: 2.6E-43
score: 146.3
NoneNo IPR availablePIRSRPIRSR000550-2PIRSR000550-2coord: 126..449
e-value: 9.8E-101
score: 335.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 57..80
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..80
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 112..147
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..45
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 122..142
NoneNo IPR availablePANTHERPTHR24351:SF202NON-SPECIFIC SERINE/THREONINE PROTEIN KINASEcoord: 7..484
NoneNo IPR availablePANTHERPTHR24351RIBOSOMAL PROTEIN S6 KINASEcoord: 7..484
NoneNo IPR availableCDDcd05123STKc_AGCcoord: 159..417
e-value: 6.89087E-152
score: 430.785
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 159..190
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 280..292
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 150..474

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg18160-RACarg18160-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity