Carg17801 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg17801
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Protein TIC 20
Location	Carg_Chr10: 4009329 .. 4010774 (-)
RNA-Seq Expression	Carg17801
Synteny	Carg17801

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCTGTTCCATCCCTCTCCTCAATCTCTCCATCAAACCCGCCATTAAAGCCGCACTAATACCTCACTACTCCGCCATCAAATCTCGCCACTGTTTTTCTCTCCCCCTTCTTCCCTCCAAACCCGCAAAATCTCCCGCCATTCGCATGTCCTACAACCCTACTCCGGCCACCGACCGCCTCATCTCCGCCGTCGCCTACACTCTCCCCTTCTTCAACTCCCTCCAGTATGGTCGCTTCCTCTTCGCTCAGTACCCGGTTCTCGGCCTCGCCTTCGAGCCGCTGCTCCCGATTCTAGGGCTTTATCGCTCGATCCCGCACTCGAGCTTCATCGCCTTCTTCGCCTTCTACTTGGGCGTGGTCCGTAACCCTAGCTTCAGTCGCTACGCGAGGTTCAATTCGATGCAGGCGTTGACTCTGGACGTGCTGCTGGTGGTGCCGTTGTTGGTACAGAGGATTCTGAGTCCCGGTCAAAGCGGATTAGGGTTCCAGATCATGGCTTGGAGCCATAATGGATTGTTTGTTTTCAGTGTTTTGTGCTTTCTTTATAGTGTGGTTTCCTGCATTCTGGGTCGAACCCCTCACCTACCCATTGTTGCTGATGCTGCTGGCAGGCAGGTTTAGCTTCAGGTTTGTTCATCAACCTTTTCTCTCTTGTTTTGGGGAATTTTTGGATTGAGAAGTTCGTAATGTAGGGTTTTGATTTTTCTCCAAAATTAATGAAGTTCTGAAAGTTTTGGGAGAAATCTGAATCCAATCAATGGAAGATTTATAAGAAACGAATTGAAAAGTTTATTTTGATTGTGCATTTCTGATTACAAGAGATTTTTGCAGCCTGTACCTATAAGTTTGTAAAGCATTTGTGTAAGGGGAAGCTTTAGATGATGAAGATCATTCATACATAGCCTTAAATACAACTTGGTCAGCGTGATTCATTTAGGTCCCATTTCATTCGGTTCACCATTACTTTGAGAATGGCCCTCTTGAACTCTGTTCTTTTCTTTGGTCCATCATTCTCATTTTTATCTCAGTTTTTGTGATCAAATTTTTAGCATGACATTCATTCAACCTTAAAAAAGTGTTTCCTGGTTCAATTTTGCAAATAGGGATCAAAATGAACAAGGATGAAATTCAAAAGTGGGATGATCGAGATAGAAAACGAGAGACGATGATACAGTAGAGAGCGATGATGATACAGTAAAGATTGGCACACACTCTTATCGAAGAAATTAGTGACAATGGGGTAATCTAATCTTGATTATTAGTATGCTTGTGCGTAAATTGATCCTCATACACATTGAAAATTCTTACCCATCATGTTGAGGATTTGAACATGTTCCATGGGAGGATGAAAGTCCCCTAATTAATTTAGGGAATGATCATGGGTTTATAACCAAAGAATACTCTCTCTATTGGTACGAGACCTTTTGGAGAAGTCCAAAG

mRNA sequence

ATGGCTTCCTGTTCCATCCCTCTCCTCAATCTCTCCATCAAACCCGCCATTAAAGCCGCACTAATACCTCACTACTCCGCCATCAAATCTCGCCACTGTTTTTCTCTCCCCCTTCTTCCCTCCAAACCCGCAAAATCTCCCGCCATTCGCATGTCCTACAACCCTACTCCGGCCACCGACCGCCTCATCTCCGCCGTCGCCTACACTCTCCCCTTCTTCAACTCCCTCCAGTATGGTCGCTTCCTCTTCGCTCAGTACCCGGTTCTCGGCCTCGCCTTCGAGCCGCTGCTCCCGATTCTAGGGCTTTATCGCTCGATCCCGCACTCGAGCTTCATCGCCTTCTTCGCCTTCTACTTGGGCGTGGTCCGTAACCCTAGCTTCAGTCGCTACGCGAGGTTCAATTCGATGCAGGCGTTGACTCTGGACGTGCTGCTGGTGGTGCCGTTGTTGGTACAGAGGATTCTGAGTCCCGGTCAAAGCGGATTAGGGTTCCAGATCATGGCTTGGAGCCATAATGGATTTGTGGTTTCCTGCATTCTGGGTCGAACCCCTCACCTACCCATTGTTGCTGATGCTGCTGGCAGGCAGCCTGTACCTATAAGGATCAAAATGAACAAGGATGAAATTCAAAAGTGGGATGATCGAGATAGAAAACGAGAGACGATGATACAGTAGAGAGCGATGATGATACAGTAAAGATTGGCACACACTCTTATCGAAGAAATTAGTGACAATGGGGTAATCTAATCTTGATTATTAGTATGCTTGTGCGTAAATTGATCCTCATACACATTGAAAATTCTTACCCATCATGTTGAGGATTTGAACATGTTCCATGGGAGGATGAAAGTCCCCTAATTAATTTAGGGAATGATCATGGGTTTATAACCAAAGAATACTCTCTCTATTGGTACGAGACCTTTTGGAGAAGTCCAAAG

Coding sequence (CDS)

ATGGCTTCCTGTTCCATCCCTCTCCTCAATCTCTCCATCAAACCCGCCATTAAAGCCGCACTAATACCTCACTACTCCGCCATCAAATCTCGCCACTGTTTTTCTCTCCCCCTTCTTCCCTCCAAACCCGCAAAATCTCCCGCCATTCGCATGTCCTACAACCCTACTCCGGCCACCGACCGCCTCATCTCCGCCGTCGCCTACACTCTCCCCTTCTTCAACTCCCTCCAGTATGGTCGCTTCCTCTTCGCTCAGTACCCGGTTCTCGGCCTCGCCTTCGAGCCGCTGCTCCCGATTCTAGGGCTTTATCGCTCGATCCCGCACTCGAGCTTCATCGCCTTCTTCGCCTTCTACTTGGGCGTGGTCCGTAACCCTAGCTTCAGTCGCTACGCGAGGTTCAATTCGATGCAGGCGTTGACTCTGGACGTGCTGCTGGTGGTGCCGTTGTTGGTACAGAGGATTCTGAGTCCCGGTCAAAGCGGATTAGGGTTCCAGATCATGGCTTGGAGCCATAATGGATTTGTGGTTTCCTGCATTCTGGGTCGAACCCCTCACCTACCCATTGTTGCTGATGCTGCTGGCAGGCAGCCTGTACCTATAAGGATCAAAATGAACAAGGATGAAATTCAAAAGTGGGATGATCGAGATAGAAAACGAGAGACGATGATACAGTAG

Protein sequence

MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLGVVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGFVVSCILGRTPHLPIVADAAGRQPVPIRIKMNKDEIQKWDDRDRKRETMIQ

Homology

[Close]

BLAST of Carg17801 vs. NCBI nr
Match: KAG7023770.1 (Protein TIC 20-II, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 443.0 bits (1138), Expect = 1.6e-120
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 60
           MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD
Sbjct: 1   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 60

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG
Sbjct: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 120

Query: 121 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGFVVSCIL 180
           VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGFVVSCIL
Sbjct: 121 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGFVVSCIL 180

Query: 181 GRTPHLPIVADAAGRQPVPIRIKMNKDEIQKWDDRDRKRETMIQ 225
           GRTPHLPIVADAAGRQPVPIRIKMNKDEIQKWDDRDRKRETMIQ
Sbjct: 181 GRTPHLPIVADAAGRQPVPIRIKMNKDEIQKWDDRDRKRETMIQ 224

[Close]

BLAST of Carg17801 vs. NCBI nr
Match: XP_022960653.1 (protein TIC 20-II, chloroplastic-like [Cucurbita moschata] >XP_022960654.1 protein TIC 20-II, chloroplastic-like [Cucurbita moschata] >KAG6590106.1 Protein TIC 20-II, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 376.7 bits (966), Expect = 1.4e-100
Identity = 195/207 (94.20%), Postives = 195/207 (94.20%), Query Frame = 0


Query: 1   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 60
           MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD
Sbjct: 3   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 62

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG
Sbjct: 63  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 122

Query: 121 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGF------ 180
           VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNG       
Sbjct: 123 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGLFVFSVL 182

Query: 181 -----VVSCILGRTPHLPIVADAAGRQ 197
                VVSCILGRTPHLPIVADAAGRQ
Sbjct: 183 CFLYSVVSCILGRTPHLPIVADAAGRQ 209

[Close]

BLAST of Carg17801 vs. NCBI nr
Match: XP_023516082.1 (protein TIC 20-II, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 372.9 bits (956), Expect = 2.0e-99
Identity = 193/207 (93.24%), Postives = 193/207 (93.24%), Query Frame = 0


Query: 1   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 60
           MASCSIPLLNLS KPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD
Sbjct: 1   MASCSIPLLNLSTKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 60

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG
Sbjct: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 120

Query: 121 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGF------ 180
           VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGF IMAWSHNG       
Sbjct: 121 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFHIMAWSHNGLFVFSVL 180

Query: 181 -----VVSCILGRTPHLPIVADAAGRQ 197
                VVSCILGRTPHLPIVADAAGRQ
Sbjct: 181 CFLYSVVSCILGRTPHLPIVADAAGRQ 207

[Close]

BLAST of Carg17801 vs. NCBI nr
Match: XP_022987499.1 (protein TIC 20-II, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 372.1 bits (954), Expect = 3.4e-99
Identity = 192/207 (92.75%), Postives = 194/207 (93.72%), Query Frame = 0


Query: 1   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 60
           MASCSIPLLNLS KPAIKAALIPHYSAIKSRHCFSLPLLPS+PAKSPAIRMSYNPTPATD
Sbjct: 3   MASCSIPLLNLSTKPAIKAALIPHYSAIKSRHCFSLPLLPSRPAKSPAIRMSYNPTPATD 62

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG
Sbjct: 63  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 122

Query: 121 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGF------ 180
           VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPG+SGLGFQIMAWSHNG       
Sbjct: 123 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGRSGLGFQIMAWSHNGLFVFSVL 182

Query: 181 -----VVSCILGRTPHLPIVADAAGRQ 197
                VVSCILGRTPHLPIVADAAGRQ
Sbjct: 183 CFLYSVVSCILGRTPHLPIVADAAGRQ 209

[Close]

BLAST of Carg17801 vs. NCBI nr
Match: XP_038879337.1 (protein TIC 20-II, chloroplastic [Benincasa hispida])

HSP 1 Score: 325.5 bits (833), Expect = 3.7e-85
Identity = 166/207 (80.19%), Postives = 177/207 (85.51%), Query Frame = 0


Query: 1   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 60
           MAS SIPLLNLS+K A +AALIPH SAIKSRH  SLP LPSKPAKS   RMSYNPTPATD
Sbjct: 1   MASSSIPLLNLSVKTAFRAALIPHSSAIKSRHRSSLPFLPSKPAKSTVFRMSYNPTPATD 60

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 120
           R+ISAVAYTLPFFNSLQYGRFLFAQYP LG AFEPLLPILGLYRSIPH+SF+AFFA YLG
Sbjct: 61  RVISAVAYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHASFVAFFALYLG 120

Query: 121 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGF------ 180
           +VRNPSFS Y RFNSMQA+TLDVLLVVPLL+QRILSPG+ GLGFQIM W HNG       
Sbjct: 121 LVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGLGFQIMVWGHNGLFVFSVL 180

Query: 181 -----VVSCILGRTPHLPIVADAAGRQ 197
                +VSC+LGRTPHLPIVADAAGRQ
Sbjct: 181 CFVYSLVSCLLGRTPHLPIVADAAGRQ 207

[Close]

BLAST of Carg17801 vs. ExPASy Swiss-Prot
Match: O82251 (Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-II PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 6.5e-53
Identity = 106/168 (63.10%), Postives = 125/168 (74.40%), Query Frame = 0


Query: 41  SKPAKSPAIRMSYNPTPATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPIL 100
           S+ A +   R SY PTPAT+R+IS  +Y LPFFNSLQYGRFLFAQYP LGL FEP+ PIL
Sbjct: 40  SRRATALTTRASYTPTPATERVISIASYALPFFNSLQYGRFLFAQYPRLGLLFEPIFPIL 99

Query: 101 GLYRSIPHSSFIAFFAFYLGVVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQ- 160
            LYRS+P++SF+AFF  YLGVVRN SFSRY RFN+MQA+TLDVLL VP+L+ RIL PGQ 
Sbjct: 100 NLYRSVPYASFVAFFGLYLGVVRNTSFSRYVRFNAMQAVTLDVLLAVPVLLTRILDPGQG 159

Query: 161 SGLGFQIMAWSHNGF-----------VVSCILGRTPHLPIVADAAGRQ 197
            G G + M W H G            VVS +LG+TP++P VADAAGRQ
Sbjct: 160 GGFGMKAMMWGHTGVFVFSFMCFVYGVVSSLLGKTPYIPFVADAAGRQ 207

[Close]

BLAST of Carg17801 vs. ExPASy Swiss-Prot
Match: Q9FM67 (Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-V PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.3e-28
Identity = 65/150 (43.33%), Postives = 93/150 (62.00%), Query Frame = 0


Query: 58  ATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAF 117
           A+DR+ISAV Y  PFF+ +QYG+F+  QY    +  +PL P +  ++S P + F+ F   
Sbjct: 59  ASDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIFITL 118

Query: 118 YLGVVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQ-IMAWSHNGFVV 177
           Y  VVRNP+FSRY RFN+MQA+ LDVLL+ P L++R  +P + G G   +M+     F+ 
Sbjct: 119 YFVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNP-RDGFGLDVVMSLDSTVFLF 178

Query: 178 ----------SCILGRTPHLPIVADAAGRQ 197
                     +C+ G TP LP+VA+AA RQ
Sbjct: 179 LLVSLIYGFSACLFGLTPRLPLVAEAADRQ 207

[Close]

BLAST of Carg17801 vs. ExPASy Swiss-Prot
Match: P73387 (Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1737 PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.3e-18
Identity = 50/147 (34.01%), Postives = 82/147 (55.78%), Query Frame = 0


Query: 60  DRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYL 119
           DR  SA+ Y +P  ++  +G FL  Q+PVL + + P++P+L  Y   P +SFI F   ++
Sbjct: 11  DRFFSALIYVIPLIDAFMFGGFLLQQFPVLQIIYLPIMPLLQFYYQFPFASFIIFIVLFM 70

Query: 120 GVVRNPSFSRYARFNSMQALTLDVL-----LVVPLLVQRILSPG---QSGLGFQIMAWSH 179
            VVRN + S + RFN+MQA+ + +L     L+V  ++Q +   G   ++   F  +    
Sbjct: 71  AVVRNNNISHFIRFNAMQAILIGILLSLFGLIVAYVIQPVFGQGLVTETLYNFAFLGALA 130

Query: 180 NGF--VVSCILGRTPHLPIVADAAGRQ 197
            GF  +V  +LGR   +P ++DAA  Q
Sbjct: 131 CGFFGIVQSVLGRYAEIPTISDAAYSQ 157

[Close]

BLAST of Carg17801 vs. ExPASy TrEMBL
Match: A0A6J1HBN6 (Protein TIC 20 OS=Cucurbita moschata OX=3662 GN=LOC111461378 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 6.7e-101
Identity = 195/207 (94.20%), Postives = 195/207 (94.20%), Query Frame = 0


Query: 1   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 60
           MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD
Sbjct: 3   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 62

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG
Sbjct: 63  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 122

Query: 121 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGF------ 180
           VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNG       
Sbjct: 123 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGLFVFSVL 182

Query: 181 -----VVSCILGRTPHLPIVADAAGRQ 197
                VVSCILGRTPHLPIVADAAGRQ
Sbjct: 183 CFLYSVVSCILGRTPHLPIVADAAGRQ 209

[Close]

BLAST of Carg17801 vs. ExPASy TrEMBL
Match: A0A6J1JJ22 (Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111485044 PE=3 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.6e-99
Identity = 192/207 (92.75%), Postives = 194/207 (93.72%), Query Frame = 0


Query: 1   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 60
           MASCSIPLLNLS KPAIKAALIPHYSAIKSRHCFSLPLLPS+PAKSPAIRMSYNPTPATD
Sbjct: 3   MASCSIPLLNLSTKPAIKAALIPHYSAIKSRHCFSLPLLPSRPAKSPAIRMSYNPTPATD 62

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG
Sbjct: 63  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 122

Query: 121 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGF------ 180
           VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPG+SGLGFQIMAWSHNG       
Sbjct: 123 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGRSGLGFQIMAWSHNGLFVFSVL 182

Query: 181 -----VVSCILGRTPHLPIVADAAGRQ 197
                VVSCILGRTPHLPIVADAAGRQ
Sbjct: 183 CFLYSVVSCILGRTPHLPIVADAAGRQ 209

[Close]

BLAST of Carg17801 vs. ExPASy TrEMBL
Match: A0A0A0LXS7 (Protein TIC 20 OS=Cucumis sativus OX=3659 GN=Csa_1G654890 PE=3 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 4.4e-84
Identity = 166/207 (80.19%), Postives = 176/207 (85.02%), Query Frame = 0


Query: 1   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPSKPAKSPAIRMSYNPTPATD 60
           MAS SIPLLNLS K +I+AAL PH SAIKSRH  SLP LPSK  K   IRMSYNPTPATD
Sbjct: 1   MASTSIPLLNLSAKTSIRAALFPHSSAIKSRHHSSLPFLPSKSPKFTPIRMSYNPTPATD 60

Query: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAFYLG 120
           RLISAVAYTLPFFNSLQYGRFLFAQYP LG AFEPLLPILGLYRSIPHSSF+AFFA YLG
Sbjct: 61  RLISAVAYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFVAFFALYLG 120

Query: 121 VVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGF------ 180
           VVRNPSF+ Y RFNSMQA+TLDVLLVVPLL+QRILSPG+SG+GF+IM WSHNG       
Sbjct: 121 VVRNPSFNHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRSGIGFKIMVWSHNGLFVFSVL 180

Query: 181 -----VVSCILGRTPHLPIVADAAGRQ 197
                VVSC+LGRTPHLPIVADAAGRQ
Sbjct: 181 CFLYSVVSCLLGRTPHLPIVADAAGRQ 207

[Close]

BLAST of Carg17801 vs. ExPASy TrEMBL
Match: A0A1S3B9C6 (Protein TIC 20 OS=Cucumis melo OX=3656 GN=LOC103487217 PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 9.7e-84
Identity = 167/210 (79.52%), Postives = 177/210 (84.29%), Query Frame = 0


Query: 1   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPS---KPAKSPAIRMSYNPTP 60
           MAS SIPLLNLS K AI+AAL PH S IKSRH FSLP LPS   K AKS  IRMSYNPTP
Sbjct: 1   MASTSIPLLNLSAKTAIRAALFPHSSTIKSRHHFSLPFLPSKSAKSAKSTPIRMSYNPTP 60

Query: 61  ATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAF 120
           ATDRLISAV+YTLPFFNSLQYGRFLFAQYP LG AFEPLLPILGLYRSIPHSSF+AFFA 
Sbjct: 61  ATDRLISAVSYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFVAFFAL 120

Query: 121 YLGVVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGF--- 180
           YLGVVRNPSFS Y RFNSMQA+TLDVLLVVPLL+QRILSPG+ G+GF+IM WSHNG    
Sbjct: 121 YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFKIMVWSHNGLFVF 180

Query: 181 --------VVSCILGRTPHLPIVADAAGRQ 197
                   +VSC+LGRTPHLPIVADAAGRQ
Sbjct: 181 SVLCFLYSMVSCLLGRTPHLPIVADAAGRQ 210

[Close]

BLAST of Carg17801 vs. ExPASy TrEMBL
Match: A0A5D3E499 (Protein TIC 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G00600 PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 9.7e-84
Identity = 167/210 (79.52%), Postives = 177/210 (84.29%), Query Frame = 0


Query: 1   MASCSIPLLNLSIKPAIKAALIPHYSAIKSRHCFSLPLLPS---KPAKSPAIRMSYNPTP 60
           MAS SIPLLNLS K AI+AAL PH S IKSRH FSLP LPS   K AKS  IRMSYNPTP
Sbjct: 1   MASTSIPLLNLSAKTAIRAALFPHSSTIKSRHHFSLPFLPSKSAKSAKSTPIRMSYNPTP 60

Query: 61  ATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAF 120
           ATDRLISAV+YTLPFFNSLQYGRFLFAQYP LG AFEPLLPILGLYRSIPHSSF+AFFA 
Sbjct: 61  ATDRLISAVSYTLPFFNSLQYGRFLFAQYPALGFAFEPLLPILGLYRSIPHSSFVAFFAL 120

Query: 121 YLGVVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQIMAWSHNGF--- 180
           YLGVVRNPSFS Y RFNSMQA+TLDVLLVVPLL+QRILSPG+ G+GF+IM WSHNG    
Sbjct: 121 YLGVVRNPSFSHYVRFNSMQAVTLDVLLVVPLLIQRILSPGRGGIGFKIMVWSHNGLFVF 180

Query: 181 --------VVSCILGRTPHLPIVADAAGRQ 197
                   +VSC+LGRTPHLPIVADAAGRQ
Sbjct: 181 SVLCFLYSMVSCLLGRTPHLPIVADAAGRQ 210

[Close]

BLAST of Carg17801 vs. TAIR 10
Match: AT2G47840.1 (Uncharacterised conserved protein ycf60 )

HSP 1 Score: 208.8 bits (530), Expect = 4.6e-54
Identity = 106/168 (63.10%), Postives = 125/168 (74.40%), Query Frame = 0


Query: 41  SKPAKSPAIRMSYNPTPATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPIL 100
           S+ A +   R SY PTPAT+R+IS  +Y LPFFNSLQYGRFLFAQYP LGL FEP+ PIL
Sbjct: 40  SRRATALTTRASYTPTPATERVISIASYALPFFNSLQYGRFLFAQYPRLGLLFEPIFPIL 99

Query: 101 GLYRSIPHSSFIAFFAFYLGVVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQ- 160
            LYRS+P++SF+AFF  YLGVVRN SFSRY RFN+MQA+TLDVLL VP+L+ RIL PGQ 
Sbjct: 100 NLYRSVPYASFVAFFGLYLGVVRNTSFSRYVRFNAMQAVTLDVLLAVPVLLTRILDPGQG 159

Query: 161 SGLGFQIMAWSHNGF-----------VVSCILGRTPHLPIVADAAGRQ 197
            G G + M W H G            VVS +LG+TP++P VADAAGRQ
Sbjct: 160 GGFGMKAMMWGHTGVFVFSFMCFVYGVVSSLLGKTPYIPFVADAAGRQ 207

[Close]

BLAST of Carg17801 vs. TAIR 10
Match: AT5G55710.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast thylakoid membrane, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: Uncharacterised conserved protein ycf60 (TAIR:AT2G47840.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 126.7 bits (317), Expect = 2.3e-29
Identity = 65/150 (43.33%), Postives = 93/150 (62.00%), Query Frame = 0


Query: 58  ATDRLISAVAYTLPFFNSLQYGRFLFAQYPVLGLAFEPLLPILGLYRSIPHSSFIAFFAF 117
           A+DR+ISAV Y  PFF+ +QYG+F+  QY    +  +PL P +  ++S P + F+ F   
Sbjct: 59  ASDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIFITL 118

Query: 118 YLGVVRNPSFSRYARFNSMQALTLDVLLVVPLLVQRILSPGQSGLGFQ-IMAWSHNGFVV 177
           Y  VVRNP+FSRY RFN+MQA+ LDVLL+ P L++R  +P + G G   +M+     F+ 
Sbjct: 119 YFVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNP-RDGFGLDVVMSLDSTVFLF 178

Query: 178 ----------SCILGRTPHLPIVADAAGRQ 197
                     +C+ G TP LP+VA+AA RQ
Sbjct: 179 LLVSLIYGFSACLFGLTPRLPLVAEAADRQ 207

The following BLAST results are available for this feature:

BLAST of Carg17801 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023770.1	1.6e-120	100.00	Protein TIC 20-II, chloroplastic, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_022960653.1	1.4e-100	94.20	protein TIC 20-II, chloroplastic-like [Cucurbita moschata] >XP_022960654.1 prote...	[more]
XP_023516082.1	2.0e-99	93.24	protein TIC 20-II, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022987499.1	3.4e-99	92.75	protein TIC 20-II, chloroplastic-like [Cucurbita maxima]	[more]
XP_038879337.1	3.7e-85	80.19	protein TIC 20-II, chloroplastic [Benincasa hispida]	[more]

BLAST of Carg17801 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82251	6.5e-53	63.10	Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-II PE=...	[more]
Q9FM67	3.3e-28	43.33	Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-V PE=2 ...	[more]
P73387	2.3e-18	34.01	Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 ...	[more]

BLAST of Carg17801 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBN6	6.7e-101	94.20	Protein TIC 20 OS=Cucurbita moschata OX=3662 GN=LOC111461378 PE=3 SV=1	[more]
A0A6J1JJ22	1.6e-99	92.75	Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111485044 PE=3 SV=1	[more]
A0A0A0LXS7	4.4e-84	80.19	Protein TIC 20 OS=Cucumis sativus OX=3659 GN=Csa_1G654890 PE=3 SV=1	[more]
A0A1S3B9C6	9.7e-84	79.52	Protein TIC 20 OS=Cucumis melo OX=3656 GN=LOC103487217 PE=3 SV=1	[more]
A0A5D3E499	9.7e-84	79.52	Protein TIC 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G006...	[more]

BLAST of Carg17801 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47840.1	4.6e-54	63.10	Uncharacterised conserved protein ycf60	[more]
AT5G55710.1	2.3e-29	43.33	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005691	Chloroplast protein import component Tic20	PFAM	PF16166	TIC20	coord: 61..193 e-value: 3.5E-32 score: 111.6
IPR005691	Chloroplast protein import component Tic20	PANTHER	PTHR33510	PROTEIN TIC 20-II, CHLOROPLASTIC	coord: 48..196
None	No IPR available	PANTHER	PTHR33510:SF5	PROTEIN TIC 20-II, CHLOROPLASTIC	coord: 48..196

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg17801-RA	Carg17801-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009706	chloroplast inner membrane
cellular_component	GO:0016021	integral component of membrane