Carg17783 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg17783
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	tRNA (adenine(58)-N(1))-methyltransferase
Location	Carg_Chr10: 3901101 .. 3902216 (+)
RNA-Seq Expression	Carg17783
Synteny	Carg17783

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGCCAACTGATGACTTGAAGAAGATATCTTTCACTCGTTCTATAAGGGATGGAGATTTAGTCATTGTTTATGAGAGACATGATGTCATGAAGGCTGTGAAAGTATGTGAAAATTCAACATTTCAGAATAGGTTTGGTATGTTTAAACATTCTGATTGGATTGGAAAGCCATTTGGGACCAAGGTGTTGAGTAACAAAGGTGGCTTTGTTTACCTATTAGCCCCAACTCCTGAATTATGGACTTTGGTTCTAAGCCACAGGACACAGATTCTATACATTGCGGACATTAGTTTTGTAATTATGTTCTTAGAGATAGTTCCTGGTTGTTTGATTCTAGAATCAGGGACTGGCAGTGGATCTTTGACGACGTCTTTAGCTAGGGCTGTAGCGCCTACCGGACATGTCTATACGTTTGATTTTCATGAACAGAGAGCTGCCTCGGCAAGGTAGGAACCGGGATCTTTTATATGTTGTCAAGTTATACCCTGCTGAGTAATTGTGTTCTATGATAATTTGCTTGTCTACCTCTTTTTTCAGAGAGGATTTTGAGAAGACTGGCTTGACCAGCTTAGTCACTGTGGGAGTTAGAGATGTTCAAGGTCAGGGCTTCCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGCTGAAACAAGACGGCGTATTATGCTCGTTCTCTCCATGTATCGAGCAAGTTCAACGGTCGGCCGAAACTCTACGATCTAAGTTTACAGGTTGTTCCGAATGACCCATTTATATACCTTCATTCCTATAGATATGTTGATATGTATTATTCACTATTGTCGTCGTCGTCGTCCCTTTTGTGTAGATATAAGAACATTTGAGGTATTGCTTCGGACTTACGAGGTTCGAGAAGGTAAAACAGAGAGCAGCCAGGTTGAAGGAGGTGGTTCTGTCGAGCTTCCACCGTGCAAGAAGCGGGTGAGATCAAAGGAAGCTAACGACACGAACGGTGATTCGAGTACGGTCATGGTTAGGCCATGTAGTGAGGCCAGAGGTCACACTGGTTATCTAACATTTGCAAGACTCAGGTGCCTCTCATAA

mRNA sequence

ATGTTGCCAACTGATGACTTGAAGAAGATATCTTTCACTCGTTCTATAAGGGATGGAGATTTAGTCATTGTTTATGAGAGACATGATGTCATGAAGGCTGTGAAAGTATGTGAAAATTCAACATTTCAGAATAGGTTTGGTATGTTTAAACATTCTGATTGGATTGGAAAGCCATTTGGGACCAAGGTGTTGAGTAACAAAGGTGGCTTTGTTTACCTATTAGCCCCAACTCCTGAATTATGGACTTTGGTTCTAAGCCACAGGACACAGATTCTATACATTGCGGACATTAGTTTTGTAATTATGTTCTTAGAGATAGTTCCTGGTTGTTTGATTCTAGAATCAGGGACTGGCAGTGGATCTTTGACGACGTCTTTAGCTAGGGCTGTAGCGCCTACCGGACATGTCTATACGTTTGATTTTCATGAACAGAGAGCTGCCTCGGCAAGAGAGGATTTTGAGAAGACTGGCTTGACCAGCTTAGTCACTGTGGGAGTTAGAGATGTTCAAGGTCAGGGCTTCCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGCTGAAACAAGACGGCGTATTATGCTCGTTCTCTCCATGTATCGAGCAAGTTCAACGGTCGGCCGAAACTCTACGATCTAAGTTTACAGATATAAGAACATTTGAGGTATTGCTTCGGACTTACGAGGTTCGAGAAGGTAAAACAGAGAGCAGCCAGGTTGAAGGAGGTGGTTCTGTCGAGCTTCCACCGTGCAAGAAGCGGGTGAGATCAAAGGAAGCTAACGACACGAACGGTGATTCGAGTACGGTCATGGTTAGGCCATGTAGTGAGGCCAGAGGTCACACTGGTTATCTAACATTTGCAAGACTCAGGTGCCTCTCATAA

Coding sequence (CDS)

ATGTTGCCAACTGATGACTTGAAGAAGATATCTTTCACTCGTTCTATAAGGGATGGAGATTTAGTCATTGTTTATGAGAGACATGATGTCATGAAGGCTGTGAAAGTATGTGAAAATTCAACATTTCAGAATAGGTTTGGTATGTTTAAACATTCTGATTGGATTGGAAAGCCATTTGGGACCAAGGTGTTGAGTAACAAAGGTGGCTTTGTTTACCTATTAGCCCCAACTCCTGAATTATGGACTTTGGTTCTAAGCCACAGGACACAGATTCTATACATTGCGGACATTAGTTTTGTAATTATGTTCTTAGAGATAGTTCCTGGTTGTTTGATTCTAGAATCAGGGACTGGCAGTGGATCTTTGACGACGTCTTTAGCTAGGGCTGTAGCGCCTACCGGACATGTCTATACGTTTGATTTTCATGAACAGAGAGCTGCCTCGGCAAGAGAGGATTTTGAGAAGACTGGCTTGACCAGCTTAGTCACTGTGGGAGTTAGAGATGTTCAAGGTCAGGGCTTCCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGCTGAAACAAGACGGCGTATTATGCTCGTTCTCTCCATGTATCGAGCAAGTTCAACGGTCGGCCGAAACTCTACGATCTAAGTTTACAGATATAAGAACATTTGAGGTATTGCTTCGGACTTACGAGGTTCGAGAAGGTAAAACAGAGAGCAGCCAGGTTGAAGGAGGTGGTTCTGTCGAGCTTCCACCGTGCAAGAAGCGGGTGAGATCAAAGGAAGCTAACGACACGAACGGTGATTCGAGTACGGTCATGGTTAGGCCATGTAGTGAGGCCAGAGGTCACACTGGTTATCTAACATTTGCAAGACTCAGGTGCCTCTCATAA

Protein sequence

MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFGTKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVGLADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLLRTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYLTFARLRCLS

Homology

[Close]

BLAST of Carg17783 vs. NCBI nr
Match: KAG7023752.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 621.7 bits (1602), Expect = 3.4e-174
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60
           MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  TKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           TKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG
Sbjct: 61  TKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300
           RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300

Query: 301 TFARLRCLS 310
           TFARLRCLS
Sbjct: 301 TFARLRCLS 309

[Close]

BLAST of Carg17783 vs. NCBI nr
Match: XP_022961161.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X2 [Cucurbita moschata] >XP_022961162.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X2 [Cucurbita moschata])

HSP 1 Score: 620.2 bits (1598), Expect = 9.9e-174
Identity = 308/309 (99.68%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60
           MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  TKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           +KVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300
           RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300

Query: 301 TFARLRCLS 310
           TFARLRCLS
Sbjct: 301 TFARLRCLS 309

[Close]

BLAST of Carg17783 vs. NCBI nr
Match: XP_022961159.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X1 [Cucurbita moschata] >XP_022961160.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X1 [Cucurbita moschata])

HSP 1 Score: 618.6 bits (1594), Expect = 2.9e-173
Identity = 307/308 (99.68%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60
           MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  TKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           +KVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300
           RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300

Query: 301 TFARLRCL 309
           TFARLRCL
Sbjct: 301 TFARLRCL 308

[Close]

BLAST of Carg17783 vs. NCBI nr
Match: XP_022987109.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 618.2 bits (1593), Expect = 3.8e-173
Identity = 307/309 (99.35%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60
           MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCE+STFQNRFGMFKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCESSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  TKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           +KVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300
           RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300

Query: 301 TFARLRCLS 310
           TFARLRCLS
Sbjct: 301 TFARLRCLS 309

[Close]

BLAST of Carg17783 vs. NCBI nr
Match: XP_023516031.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 617.1 bits (1590), Expect = 8.4e-173
Identity = 306/309 (99.03%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60
           MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  TKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           +KVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300
           RTYEVREGKTESSQVEGGGSVELPPCKKRVRSK+AN+TNGDSSTVMVRPCSEARGHTGYL
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKQANNTNGDSSTVMVRPCSEARGHTGYL 300

Query: 301 TFARLRCLS 310
           TFARLRCLS
Sbjct: 301 TFARLRCLS 309

[Close]

BLAST of Carg17783 vs. ExPASy Swiss-Prot
Match: Q80XC2 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus musculus OX=10090 GN=Trmt61a PE=1 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 7.4e-63
Identity = 136/297 (45.79%), Postives = 187/297 (62.96%), Query Frame = 0


Query: 10  ISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHS-DWIGKPFGTKVLSNKG 69
           +++   I++GD  I+   H  M AV+V   +  Q R G+ +HS D IG+PFG+KV+ ++G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVICSRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLAR 129
           G+VY+L PTPELWT+ L HRTQILY  DI+ + M LE+ PG ++ ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVGLADSVFLD 189
           +VAPTGH++T +FH+QRA  ARE+F++  L+  VTV  +DV   GF    V +AD+VFLD
Sbjct: 124 SVAPTGHLHTVEFHQQRADKAREEFQEHRLSQWVTVHTQDVCCSGF--GVVHVADAVFLD 183

Query: 190 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVR 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  FT++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR 243

Query: 250 EGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYLTFA 304
                        +V LP       + E N  +  S      P  E  GHTGYLTFA
Sbjct: 244 -------------TVSLPLPDLGANNLETNMGSDASPFRSGTPMKETVGHTGYLTFA 285

[Close]

BLAST of Carg17783 vs. ExPASy Swiss-Prot
Match: Q96FX7 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapiens OX=9606 GN=TRMT61A PE=1 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 6.2e-62
Identity = 137/300 (45.67%), Postives = 184/300 (61.33%), Query Frame = 0


Query: 10  ISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHS-DWIGKPFGTKVLSNKG 69
           +++   I++GD  I+   H  M AV+V   +  Q R G+ +HS D IG+PFG+KV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLAR 129
           G+VY+L PTPELWTL L HRTQILY  DI+ + M LE+ PG ++ ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVGLADSVFLD 189
            +APTGH++T +FH+QRA  ARE+F++  +   VTV  +DV   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHV--ADAVFLD 183

Query: 190 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVR 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L ++ F+++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVR 243

Query: 250 EGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVR---PCSEARGHTGYLTFA 304
                        +V LPP           D    S T   R   P  EA GHTGYLTFA
Sbjct: 244 -------------TVSLPPPDLGT----GTDGPAGSDTSPFRSGTPMKEAVGHTGYLTFA 284

[Close]

BLAST of Carg17783 vs. ExPASy Swiss-Prot
Match: Q6AY46 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus norvegicus OX=10116 GN=Trmt61a PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.8e-61
Identity = 133/297 (44.78%), Postives = 185/297 (62.29%), Query Frame = 0


Query: 10  ISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHS-DWIGKPFGTKVLSNKG 69
           +++   I++GD  I+   H  M AV+V   +  Q R G+ +HS D IG+PFG+KV  ++G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCSRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLAR 129
           G+VY+L PTPELWT+ L HRTQILY  DI+ + M LE+ PG ++ ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVGLADSVFLD 189
           ++APTGH++T +FH+QRA  ARE+F++  ++  VTV  +DV   GF    V +AD+VFLD
Sbjct: 124 SIAPTGHLHTVEFHQQRADKAREEFQQHRVSQWVTVHTQDVCRSGF--GVVHVADAVFLD 183

Query: 190 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVR 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  FT++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR 243

Query: 250 EGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYLTFA 304
                        +V LP         E N  +  +      P  E  GHTGYLTFA
Sbjct: 244 -------------TVSLPLPDLGADDLEGNVGSDATPFRSGTPMKETVGHTGYLTFA 285

[Close]

BLAST of Carg17783 vs. ExPASy Swiss-Prot
Match: Q86JJ0 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyostelium discoideum OX=44689 GN=trmt61a PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.6e-60
Identity = 130/310 (41.94%), Postives = 190/310 (61.29%), Query Frame = 0


Query: 16  IRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFGTKVLSNKG-GFVYLL 75
           I++GD V++Y   D M  + +  N+ + ++FG ++H + IGK +G+K+ S+ G GF +++
Sbjct: 8   IKEGDRVVMYNGKDNMAVLTMESNNVYNSKFGSYRHKNIIGKEYGSKLSSDNGNGFCHVI 67

Query: 76  APTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLARAVAPTG 135
           A TPELW++ L HRTQIL+  DIS +I  LE+  G   +ESGTGSGSL++S+AR +AP G
Sbjct: 68  AMTPELWSITLDHRTQILFNLDISTIIFNLELKNGSRAVESGTGSGSLSSSIARTIAPKG 127

Query: 136 HVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQG-QGFPEEFVGL-ADSVFLDLPQP 195
           H++TF+FHE+R   AR+DF+  GL   +TV  RD  G +GF  + +    D+VFLDLP P
Sbjct: 128 HLFTFEFHEERVKFARKDFKDNGLDQYITVTHRDACGKEGFLRQDINNDIDAVFLDLPSP 187

Query: 196 WLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLR-SKFTDIRTFEVLLRTYEVREGKTE 255
           W AI +A  ++    +LCSFSPCIEQVQ +   L  SKF +I+T EVL+RT++ R  + E
Sbjct: 188 WDAIENAIAVMHDGSMLCSFSPCIEQVQNTCLKLADSKFQEIKTIEVLIRTFDTRLQEYE 247

Query: 256 SSQVEGGGSVELPPCKKRVRSKEANDTNGD-----------------SSTVMVRPCSEAR 305
                     EL      + +   N+ N +                    ++ +P +EAR
Sbjct: 248 ----------ELNLTNPYIDNNNNNNNNNNIEENRGKFEIGGIEGLKKDKLLSKPFTEAR 307

[Close]

BLAST of Carg17783 vs. ExPASy Swiss-Prot
Match: A6H791 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos taurus OX=9913 GN=TRMT61A PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.9e-59
Identity = 133/297 (44.78%), Postives = 181/297 (60.94%), Query Frame = 0


Query: 10  ISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHS-DWIGKPFGTKVLSNKG 69
           +++   I++GD  I+   H  M AV+V   +  Q R G+ +HS D IG+PFG+KV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLAR 129
           G+VY+L PTPELWTL L HRTQILY  DI+ + M LE+ PG ++ ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALLTMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVGLADSVFLD 189
            +APTGH++T +FH+QRA  ARE+F++  +   VTV  +DV   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAERAREEFQEHRVGRWVTVLNQDVCRSGFGVSHV--ADAVFLD 183

Query: 190 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRS-KFTDIRTFEVLLRTYEVR 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  F+++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAACGFSELSTLEVLPQVYNVR 243

Query: 250 EGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYLTFA 304
                        S+ +P    R     A   +G        P  E  GHTGYLTFA
Sbjct: 244 T-----------VSLPVPDLGARPGPDAAPFRSG-------TPMKETVGHTGYLTFA 280

[Close]

BLAST of Carg17783 vs. ExPASy TrEMBL
Match: A0A6J1H9F4 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461754 PE=3 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 4.8e-174
Identity = 308/309 (99.68%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60
           MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  TKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           +KVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300
           RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300

Query: 301 TFARLRCLS 310
           TFARLRCLS
Sbjct: 301 TFARLRCLS 309

[Close]

BLAST of Carg17783 vs. ExPASy TrEMBL
Match: A0A6J1HBB8 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461754 PE=3 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 1.4e-173
Identity = 307/308 (99.68%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60
           MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  TKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           +KVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300
           RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300

Query: 301 TFARLRCL 309
           TFARLRCL
Sbjct: 301 TFARLRCL 308

[Close]

BLAST of Carg17783 vs. ExPASy TrEMBL
Match: A0A6J1JHY4 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484759 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 1.8e-173
Identity = 307/309 (99.35%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60
           MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCE+STFQNRFGMFKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCESSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  TKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           +KVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300
           RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300

Query: 301 TFARLRCLS 310
           TFARLRCLS
Sbjct: 301 TFARLRCLS 309

[Close]

BLAST of Carg17783 vs. ExPASy TrEMBL
Match: A0A6J1J9F9 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484759 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 5.3e-173
Identity = 306/308 (99.35%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60
           MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCE+STFQNRFGMFKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCESSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  TKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           +KVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300
           RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSSTVMVRPCSEARGHTGYL 300

Query: 301 TFARLRCL 309
           TFARLRCL
Sbjct: 301 TFARLRCL 308

[Close]

BLAST of Carg17783 vs. ExPASy TrEMBL
Match: A0A0A0LYQ8 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 3.6e-161
Identity = 282/310 (90.97%), Postives = 301/310 (97.10%), Query Frame = 0


Query: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60
           MLPTD+LK ISFTRSIRDGDLVIVYERHD+MKAVKVC+ ST QNRFG+FKHSDWIGKPFG
Sbjct: 396 MLPTDELKSISFTRSIRDGDLVIVYERHDIMKAVKVCDGSTLQNRFGVFKHSDWIGKPFG 455

Query: 61  TKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           +K LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 456 SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 515

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRA+SAREDFEKTGLTSLVTVGVRD+QGQGFPEEFVG
Sbjct: 516 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 575

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDG+LCSFSPCIEQVQRS ETLRSKFTDIRTFEVLL
Sbjct: 576 LADSVFLDLPQPWLAIPSAERMLKQDGMLCSFSPCIEQVQRSVETLRSKFTDIRTFEVLL 635

Query: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSS-TVMVRPCSEARGHTGY 300
           RTYEV+EG+TES+QV+GGGSV LPPCK+R+RSKE++D + +SS TVMVRPCSEARGHTGY
Sbjct: 636 RTYEVKEGRTESNQVDGGGSVGLPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 695

Query: 301 LTFARLRCLS 310
           LTFARLRCLS
Sbjct: 696 LTFARLRCLS 705

[Close]

BLAST of Carg17783 vs. TAIR 10
Match: AT5G14600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 477.2 bits (1227), Expect = 9.7e-135
Identity = 238/319 (74.61%), Postives = 269/319 (84.33%), Query Frame = 0


Query: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60
           MLPT+  K  SF R I DGDLVIVYERHDVMK VKV ++   QNRFG++KHSDWIGKP G
Sbjct: 1   MLPTESKKAFSFKRCIEDGDLVIVYERHDVMKPVKVSKDGVLQNRFGVYKHSDWIGKPLG 60

Query: 61  TKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           TKV SNKG FVYLLAPTPELWTLVLSHRTQILYIADISFV+M+LE+VPGC++LESGTGSG
Sbjct: 61  TKVFSNKGKFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEVVPGCVVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180
           SL+TSLARAVAPTGHVY+FDFHEQRA SAREDFEKTG++SLVTV VRD+QG+GFPE+  G
Sbjct: 121 SLSTSLARAVAPTGHVYSFDFHEQRAVSAREDFEKTGISSLVTVEVRDIQGEGFPEKLSG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLA+PSA +MLKQDGVLCSFSPCIEQVQR+ E LRS F +IRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAVPSAAKMLKQDGVLCSFSPCIEQVQRTCEVLRSDFIEIRTFEVLL 240

Query: 241 RTYEVREGKTESSQVEGGGSVE----LPPCKKRVRSKEANDT------NGDSSTVMVRPC 300
           RTYEV+E K ++S + G    E    + PCK++ RS E NDT      +  SS VM RPC
Sbjct: 241 RTYEVKEVKMDTSSMVGESHEEDNEGMRPCKRKHRSNE-NDTVSQDNSSNASSVVMARPC 300

Query: 301 SEARGHTGYLTFARLRCLS 310
           SEARGHTGYLTFARL+CL+
Sbjct: 301 SEARGHTGYLTFARLQCLA 318

The following BLAST results are available for this feature:

BLAST of Carg17783 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023752.1	3.4e-174	100.00	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A, partial [Cu...	[more]
XP_022961161.1	9.9e-174	99.68	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X2 [...	[more]
XP_022961159.1	2.9e-173	99.68	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X1 [...	[more]
XP_022987109.1	3.8e-173	99.35	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A-like isoform...	[more]
XP_023516031.1	8.4e-173	99.03	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X3 [...	[more]

BLAST of Carg17783 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q80XC2	7.4e-63	45.79	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus muscu...	[more]
Q96FX7	6.2e-62	45.67	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapi...	[more]
Q6AY46	1.8e-61	44.78	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus no...	[more]
Q86JJ0	2.6e-60	41.94	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyoste...	[more]
A6H791	4.9e-59	44.78	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos tauru...	[more]

BLAST of Carg17783 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H9F4	4.8e-174	99.68	tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1HBB8	1.4e-173	99.68	tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1JHY4	1.8e-173	99.35	tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1J9F9	5.3e-173	99.35	tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A0A0LYQ8	3.6e-161	90.97	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3...	[more]

BLAST of Carg17783 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G14600.1	9.7e-135	74.61	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014816	tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14	PFAM	PF08704	GCD14	coord: 69..304 e-value: 3.8E-97 score: 324.8
IPR014816	tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14	PIRSF	PIRSF017269	GCD14	coord: 10..308 e-value: 1.1E-98 score: 327.6
IPR014816	tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14	PANTHER	PTHR12133	TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE	coord: 2..306
IPR014816	tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14	PROSITE	PS51620	SAM_TRM61	coord: 19..306 score: 98.555634
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 78..307 e-value: 1.5E-80 score: 271.9
None	No IPR available	GENE3D	3.10.330.20		coord: 10..77 e-value: 1.3E-25 score: 91.2
None	No IPR available	PIRSR	PIRSR017269-1	PIRSR017269-1	coord: 47..250 e-value: 7.7E-41 score: 137.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 249..284
None	No IPR available	PANTHER	PTHR12133:SF2	TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61A	coord: 2..306
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 15..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg17783-RA	Carg17783-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0031515	tRNA (m1A) methyltransferase complex
molecular_function	GO:0016429	tRNA (adenine-N1-)-methyltransferase activity