Carg17004 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg17004
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMajor latex allergen Hev b 5-like
LocationCarg_Chr11: 7136789 .. 7137345 (-)
RNA-Seq ExpressionCarg17004
SyntenyCarg17004
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGCCTGTGCCACCAAGCCTAAGGCCAAGGTAGATACGGCCGTCATCCCTGCACCCGAGCCTGTCGACGACATGGTGAAGAACACCGAGGAAGTTAAGGTCGTGGAAGACGAGAAGAGGGTCGATGATGTAATTGATGAAAAGAAGAAGGACGACACCGAGAAAGTTGTCGTTGAGGAAGCCAAGCCTCCGTCTCTCGACTCCGCCAAGCCTCCGTCTCTCGACTCCGCCAAGCCTACGTCTCTCGACTCCGCCAAGCCTACGTCTCTCGACTCCGACAAGCCTCGGTCTCTCGACTCCGACAAGCCTCGGTCTCTCGACTCCGACAAGCCTCGGTCTCTCGACTCCTTGCTCGTTGAGGTATAATCCTAATTCATATTCAAAGTCATTTCTATTTATTTTCTAATATGCATTGCCTTCTATAGAAGGAAGCTGAAGTTCCGAGCTTGACGAAGGCGGAGGAGCAGCCACCGAAGGAGGCGGCGGCAGTGGCAGGGGCGAAAGAAGTGAAGAGTGAAGCGAAAGCAGCGAAGAAGGATGAGAAGAGCGCATGA

mRNA sequence

ATGGGTGCCTGTGCCACCAAGCCTAAGGCCAAGGTAGATACGGCCGTCATCCCTGCACCCGAGCCTGTCGACGACATGGTGAAGAACACCGAGGAAGTTAAGGTCGTGGAAGACGAGAAGAGGGTCGATGATGTAATTGATGAAAAGAAGAAGGACGACACCGAGAAAGTTGTCGTTGAGGAAGCCAAGCCTCCGTCTCTCGACTCCGCCAAGCCTCCGTCTCTCGACTCCGCCAAGCCTACGTCTCTCGACTCCGCCAAGCCTACGTCTCTCGACTCCGACAAGCCTCGGTCTCTCGACTCCGACAAGCCTCGGTCTCTCGACTCCGACAAGCCTCGGTCTCTCGACTCCTTGCTCGTTGAGAAGGAAGCTGAAGTTCCGAGCTTGACGAAGGCGGAGGAGCAGCCACCGAAGGAGGCGGCGGCAGTGGCAGGGGCGAAAGAAGTGAAGAGTGAAGCGAAAGCAGCGAAGAAGGATGAGAAGAGCGCATGA

Coding sequence (CDS)

ATGGGTGCCTGTGCCACCAAGCCTAAGGCCAAGGTAGATACGGCCGTCATCCCTGCACCCGAGCCTGTCGACGACATGGTGAAGAACACCGAGGAAGTTAAGGTCGTGGAAGACGAGAAGAGGGTCGATGATGTAATTGATGAAAAGAAGAAGGACGACACCGAGAAAGTTGTCGTTGAGGAAGCCAAGCCTCCGTCTCTCGACTCCGCCAAGCCTCCGTCTCTCGACTCCGCCAAGCCTACGTCTCTCGACTCCGCCAAGCCTACGTCTCTCGACTCCGACAAGCCTCGGTCTCTCGACTCCGACAAGCCTCGGTCTCTCGACTCCGACAAGCCTCGGTCTCTCGACTCCTTGCTCGTTGAGAAGGAAGCTGAAGTTCCGAGCTTGACGAAGGCGGAGGAGCAGCCACCGAAGGAGGCGGCGGCAGTGGCAGGGGCGAAAGAAGTGAAGAGTGAAGCGAAAGCAGCGAAGAAGGATGAGAAGAGCGCATGA

Protein sequence

MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVEEAKPPSLDSAKPPSLDSAKPTSLDSAKPTSLDSDKPRSLDSDKPRSLDSDKPRSLDSLLVEKEAEVPSLTKAEEQPPKEAAAVAGAKEVKSEAKAAKKDEKSA
Homology
BLAST of Carg17004 vs. NCBI nr
Match: KAG7022371.1 (hypothetical protein SDJN02_16102, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 283.1 bits (723), Expect = 1.5e-72
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0

Query: 1   MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE 60
           MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE
Sbjct: 1   MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE 60

Query: 61  EAKPPSLDSAKPPSLDSAKPTSLDSAKPTSLDSDKPRSLDSDKPRSLDSDKPRSLDSLLV 120
           EAKPPSLDSAKPPSLDSAKPTSLDSAKPTSLDSDKPRSLDSDKPRSLDSDKPRSLDSLLV
Sbjct: 61  EAKPPSLDSAKPPSLDSAKPTSLDSAKPTSLDSDKPRSLDSDKPRSLDSDKPRSLDSLLV 120

Query: 121 EKEAEVPSLTKAEEQPPKEAAAVAGAKEVKSEAKAAKKDEKSA 164
           EKEAEVPSLTKAEEQPPKEAAAVAGAKEVKSEAKAAKKDEKSA
Sbjct: 121 EKEAEVPSLTKAEEQPPKEAAAVAGAKEVKSEAKAAKKDEKSA 163

BLAST of Carg17004 vs. NCBI nr
Match: KAG6588522.1 (hypothetical protein SDJN03_17087, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 249.2 bits (635), Expect = 2.4e-62
Identity = 160/213 (75.12%), Postives = 160/213 (75.12%), Query Frame = 0

Query: 1   MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE 60
           MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE
Sbjct: 1   MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE 60

Query: 61  EAKPPSLDSAKPPSLDSAKPTSLDSA---------------------------------- 120
           EAKPPSLDSAKPPSLDSAKP SLDSA                                  
Sbjct: 61  EAKPPSLDSAKPPSLDSAKPPSLDSAKPPSLDSDKPRSLDSDKPRSLDSDKPRSLDSDKP 120

Query: 121 --------------KPTSLDSDKPRSLDSDKPRSLDSDKPRSLDSLLVEKEAEVPSLTKA 164
                         KP SLDSDKPRSLDSDKPRSLDSDKPRSLDSLLVEKEAEVPSLTKA
Sbjct: 121 RSLDFDKPRSLDSDKPRSLDSDKPRSLDSDKPRSLDSDKPRSLDSLLVEKEAEVPSLTKA 180

BLAST of Carg17004 vs. NCBI nr
Match: XP_023531836.1 (coiled-coil domain-containing protein 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 213.4 bits (542), Expect = 1.5e-51
Identity = 152/252 (60.32%), Postives = 154/252 (61.11%), Query Frame = 0

Query: 1   MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE 60
           MGACATKPKAKVDTAVIPAPE VDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE
Sbjct: 1   MGACATKPKAKVDTAVIPAPERVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE 60

Query: 61  EAKPPSLDSAKPPSLDSAKPT--------------------------------------- 120
           EAKPPSLDSAKP SLDSAKPT                                       
Sbjct: 61  EAKPPSLDSAKPTSLDSAKPTSLDSAKPRSLDSAKPRSLDSAKPRSLDSAKPRSLDSAKP 120

Query: 121 -------------------------------------------------SLDSAKPTSLD 164
                                                            SLDSAKP SLD
Sbjct: 121 RSLDSAKPRSLDSAKPRSLDSAKPRSLDSAKPRSLDSAKPRSLDSAKPRSLDSAKPRSLD 180

BLAST of Carg17004 vs. NCBI nr
Match: XP_022970143.1 (uncharacterized protein LOC111469128 [Cucurbita maxima])

HSP 1 Score: 179.5 bits (454), Expect = 2.4e-41
Identity = 114/156 (73.08%), Postives = 119/156 (76.28%), Query Frame = 0

Query: 1   MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE 60
           MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVD VIDE  KDDTE VVVE
Sbjct: 1   MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDGVIDE--KDDTENVVVE 60

Query: 61  EAKPPSLDSAKPPSLDSAKPTSLDSAKPTSLDSDKPRSLDSDKPRSLDSDKPRSLDSLLV 120
           EAKPPSLDSAKPPSLDSA P SLDSAKP                         SLDSLLV
Sbjct: 61  EAKPPSLDSAKPPSLDSATPPSLDSAKPP------------------------SLDSLLV 120

Query: 121 EKEAEVPSLTKAEEQPPKEAAAVAGAKEVKSEAKAA 157
           EK+AEVPS+TKAEEQPPK  A VAGA+EVK   ++A
Sbjct: 121 EKQAEVPSVTKAEEQPPKGTAVVAGAEEVKKNERSA 130

BLAST of Carg17004 vs. NCBI nr
Match: XP_022931727.1 (patellin-1-like [Cucurbita moschata])

HSP 1 Score: 167.9 bits (424), Expect = 7.1e-38
Identity = 112/164 (68.29%), Postives = 114/164 (69.51%), Query Frame = 0

Query: 1   MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE 60
           MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE
Sbjct: 1   MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE 60

Query: 61  EAKPPSLDSAKPPSLDSAKPTSLDSAKPTSLDSDKPRSLDSDKPRSLDSDKPRSLDSLLV 120
           EAKPP                                                SLDSLLV
Sbjct: 61  EAKPP------------------------------------------------SLDSLLV 116

Query: 121 EKEAEVPSLTKAEEQPPKEAAAVAGAKEV-KSEAKAAKKDEKSA 164
           EKEAEVPS+TKAEEQPPKEAAAVAGAKEV KSE KAAK+DEKSA
Sbjct: 121 EKEAEVPSVTKAEEQPPKEAAAVAGAKEVKKSEEKAAKRDEKSA 116

BLAST of Carg17004 vs. ExPASy TrEMBL
Match: A0A6J1I303 (uncharacterized protein LOC111469128 OS=Cucurbita maxima OX=3661 GN=LOC111469128 PE=4 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 1.1e-41
Identity = 114/156 (73.08%), Postives = 119/156 (76.28%), Query Frame = 0

Query: 1   MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE 60
           MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVD VIDE  KDDTE VVVE
Sbjct: 1   MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDGVIDE--KDDTENVVVE 60

Query: 61  EAKPPSLDSAKPPSLDSAKPTSLDSAKPTSLDSDKPRSLDSDKPRSLDSDKPRSLDSLLV 120
           EAKPPSLDSAKPPSLDSA P SLDSAKP                         SLDSLLV
Sbjct: 61  EAKPPSLDSAKPPSLDSATPPSLDSAKPP------------------------SLDSLLV 120

Query: 121 EKEAEVPSLTKAEEQPPKEAAAVAGAKEVKSEAKAA 157
           EK+AEVPS+TKAEEQPPK  A VAGA+EVK   ++A
Sbjct: 121 EKQAEVPSVTKAEEQPPKGTAVVAGAEEVKKNERSA 130

BLAST of Carg17004 vs. ExPASy TrEMBL
Match: A0A6J1EUI1 (patellin-1-like OS=Cucurbita moschata OX=3662 GN=LOC111437877 PE=4 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 3.4e-38
Identity = 112/164 (68.29%), Postives = 114/164 (69.51%), Query Frame = 0

Query: 1   MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE 60
           MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE
Sbjct: 1   MGACATKPKAKVDTAVIPAPEPVDDMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVVVE 60

Query: 61  EAKPPSLDSAKPPSLDSAKPTSLDSAKPTSLDSDKPRSLDSDKPRSLDSDKPRSLDSLLV 120
           EAKPP                                                SLDSLLV
Sbjct: 61  EAKPP------------------------------------------------SLDSLLV 116

Query: 121 EKEAEVPSLTKAEEQPPKEAAAVAGAKEV-KSEAKAAKKDEKSA 164
           EKEAEVPS+TKAEEQPPKEAAAVAGAKEV KSE KAAK+DEKSA
Sbjct: 121 EKEAEVPSVTKAEEQPPKEAAAVAGAKEVKKSEEKAAKRDEKSA 116

BLAST of Carg17004 vs. ExPASy TrEMBL
Match: A0A5A7TGB5 (Major latex allergen Hev b 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G00890 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 7.2e-12
Identity = 49/71 (69.01%), Postives = 57/71 (80.28%), Query Frame = 0

Query: 1  MGACATKPKAKV-DTAVIPAPEPVD-DMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVV 60
          MGACATKPK K  D A IPAPEP+D D+VKN EE+KVVE+EK+VD   DEKKK+D EKV+
Sbjct: 1  MGACATKPKVKTDDLATIPAPEPIDHDIVKNNEEIKVVEEEKKVDQT-DEKKKEDNEKVI 60

Query: 61 VEEAKPPSLDS 70
          VE+ K PSL S
Sbjct: 61 VEKEKSPSLAS 70

BLAST of Carg17004 vs. ExPASy TrEMBL
Match: A0A0A0K3E6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G230950 PE=4 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 6.1e-11
Identity = 77/170 (45.29%), Postives = 88/170 (51.76%), Query Frame = 0

Query: 1   MGACATKPKAKV-DTAVIPAPEPVD-DMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVV 60
           MGACATKPK K  D A IPAPEP+D D+VKN EEVK VE+EK+ D   DEKKK++ EKVV
Sbjct: 1   MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQT-DEKKKENNEKVV 60

Query: 61  VEEAKPPSLDSAKPPSLDSAKPTSLDSAKPTSLDSDKPRSLDSDKPRSLDSDKPRSLDSL 120
           V+E KPP                                                SL SL
Sbjct: 61  VKEKKPP------------------------------------------------SLASL 120

Query: 121 LVEKEA----EVPSLTKAEEQP-PKEAAAVAGAKEV-KSEAKAAKKDEKS 163
           LVEKEA    EV  + KAEE+P  KE AA     E+ KSE K  +KDEKS
Sbjct: 121 LVEKEAAPAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDEKS 121

BLAST of Carg17004 vs. ExPASy TrEMBL
Match: A0A1S3BGT9 (major latex allergen Hev b 5-like OS=Cucumis melo OX=3656 GN=LOC103489688 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 2.0e-09
Identity = 71/171 (41.52%), Postives = 86/171 (50.29%), Query Frame = 0

Query: 1   MGACATKPKAKV-DTAVIPAPEPVD-DMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVV 60
           MGACATKPK K  D A IPAPEP+D D+VKN EE+KVVE+EK+VD   DEKKK+D EKV+
Sbjct: 1   MGACATKPKVKTDDLAAIPAPEPIDHDIVKNNEEIKVVEEEKKVDQT-DEKKKEDNEKVI 60

Query: 61  VEEAKPPSLDSAKPPSLDSAKPTSLDSAKPTSLDSDKPRSLDSDKPRSLDSDKPRSLDSL 120
           VE+ K P                                                SL SL
Sbjct: 61  VEKEKSP------------------------------------------------SLASL 120

Query: 121 LVEKE-----AEVPSLTKAEEQPPKE--AAAVAGAKEVKSEAKAAKKDEKS 163
           LVEKE      EV  + KA+E+P  E   A    A+  +SE K  +K+EKS
Sbjct: 121 LVEKETAPPVVEVSCIPKADEKPKVEEVVAPTNEAEAKESEEKPKEKNEKS 122

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022371.11.5e-72100.00hypothetical protein SDJN02_16102, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6588522.12.4e-6275.12hypothetical protein SDJN03_17087, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023531836.11.5e-5160.32coiled-coil domain-containing protein 8-like [Cucurbita pepo subsp. pepo][more]
XP_022970143.12.4e-4173.08uncharacterized protein LOC111469128 [Cucurbita maxima][more]
XP_022931727.17.1e-3868.29patellin-1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1I3031.1e-4173.08uncharacterized protein LOC111469128 OS=Cucurbita maxima OX=3661 GN=LOC111469128... [more]
A0A6J1EUI13.4e-3868.29patellin-1-like OS=Cucurbita moschata OX=3662 GN=LOC111437877 PE=4 SV=1[more]
A0A5A7TGB57.2e-1269.01Major latex allergen Hev b 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A0A0K3E66.1e-1145.29Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G230950 PE=4 SV=1[more]
A0A1S3BGT92.0e-0941.52major latex allergen Hev b 5-like OS=Cucumis melo OX=3656 GN=LOC103489688 PE=4 S... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..163
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 90..120
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..89

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg17004-RACarg17004-RAmRNA