Carg16971 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16971
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Location	Carg_Chr10: 5232710 .. 5234340 (+)
RNA-Seq Expression	Carg16971
Synteny	Carg16971

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTAAAACGCATCTACTAGGAGAAGGTTTCCACACCCTTATAAATGGTGGTTTGTTCTCCCTCTCCAATCCGACGAGTGACATCACATCAATATTCTTTTTAGGTGGAAAACTAAATAAAAATGGAATATAGAAAGCTTTTTTCGTCCCTACCTTAAAATAAAATATATATCAAAATTAAAATTTAACCTTTCTTTTAGGGGAAAAAAAAAGCTAGAGCAGAAGAAGACATACCATCCTTTCACATGACAAAATAGCTCCTTGATAAACGCAGAGAATCATTTGGCCTCTCCTCTTCCCTCTCCCTTTTAACACCTTTGCTTCCGTTCTCCTCCTTCCCCTGCAAGTTCAGTCCTAAACTGACACACCAAAACACCCAACAGCTCCATTCCCACCATCTCCCTCTCTCTGTTCTTCACCATCTCCAATGGCTCTCTTTGTCTTCTGCAATCTGCTGCTTCTCCTGCTAGCTCCTTTTGCAGCCTCTAACATAGACCAAGATAGAACTGAGTGCTCAGACCAACTCATCGGCCTTGCCCCTTGCCTGCCTTATGTTAGCGGCGATGCCAAAACGCCTACCATTGATTGCTGCAGTGGGCTCAAGCAGGTGGTGCAGAAGAGTAAGAAGTGCCTCTGTGTTCTTATCAAGGATAAGGATGACCCTAATCTTGGCCTCAAAATCAATGGCTCCCTCGCTTTGGGTCTCCCTAATGCCTGCCATGCTCCTTCTAATATCACTGAGTGTATCTGTAAGTTAACTAATCTCATGGTTTTCTACTGTTTCCTGTGTTTTTCATCACTGTATTTCTCACTTGGGCATTGATCAAACACCTTGAGTATGAAATCTGTCGTCGTTGGGGAGGAGAACGAAATCTTGGGTTTTAAAAACGTCGAGAGGAAACCCGGAAGGGGAAAATTAGAAAATAATATCGATTAGCGGTGGACTTGGTCTTTAAAAACCTTGGGAATAAGCTCAAAAGAGAAAAATCAGAGAACAATAATATCTGCTAGCTGTGTGATTGGGTTTTAACAATCTTGAAGGGAAATTCACACCGGACTAATCAGAGAACAATATTTGCTAGCGATGGACTTAGGATCGAGTTGTAGTTGTTTGTACTCAAATTTCATGGTTTCCCACAGAATTTCTCAGTTGGGCATTTTGTCAAACACCTTGAGTGCATCAGTATTAAAAAAATTCCACGGCTCTGCACTCTAATTCTCAGCTGGGCATTTTGTCAAACACCTTCTGGGTACTGTATTTAACCATGCTTTGTATATGGATTTTCAGCTCTTTTGCACTTAGCACCAAACTCTACAGAAGCTAAGATATTCAAAGAGTCAAATTCCACAAGTGAACCAAGCAGTGCTCCAGATTCTAAAGGTAAAAACCAAACCCAAAATTTCCATCTAACTTAACAGAAAATCATTACCTTAGAGTAGATTGAATCGGTTTTGAACTGTTTCTCTCTGTTCGGTGATTTTGATTTACAGTTCATGGGAGTGCGTCCACTGCCAACGAGAAAAGCGACGGTGGAACGAGCAAGAAACCGATTGGAGGAGCAGGGATGATTATGGAGATTCTGCTAGGGTTCTTCATTCTATCGCATCTCAATAATCTAAAGTTCATATAG

mRNA sequence

CTTTAAAACGCATCTACTAGGAGAAGGTTTCCACACCCTTATAAATGGTGGTTTGTTCTCCCTCTCCAATCCGACGAGTGACATCACATCAATATTCTTTTTAGGTGGAAAACTAAATAAAAATGGAATATAGAAAGCTTTTTTCGTCCCTACCTTAAAATAAAATATATATCAAAATTAAAATTTAACCTTTCTTTTAGGGGAAAAAAAAAGCTAGAGCAGAAGAAGACATACCATCCTTTCACATGACAAAATAGCTCCTTGATAAACGCAGAGAATCATTTGGCCTCTCCTCTTCCCTCTCCCTTTTAACACCTTTGCTTCCGTTCTCCTCCTTCCCCTGCAAGTTCAGTCCTAAACTGACACACCAAAACACCCAACAGCTCCATTCCCACCATCTCCCTCTCTCTGTTCTTCACCATCTCCAATGGCTCTCTTTGTCTTCTGCAATCTGCTGCTTCTCCTGCTAGCTCCTTTTGCAGCCTCTAACATAGACCAAGATAGAACTGAGTGCTCAGACCAACTCATCGGCCTTGCCCCTTGCCTGCCTTATGTTAGCGGCGATGCCAAAACGCCTACCATTGATTGCTGCAGTGGGCTCAAGCAGGTGGTGCAGAAGAGTAAGAAGTGCCTCTGTGTTCTTATCAAGGATAAGGATGACCCTAATCTTGGCCTCAAAATCAATGGCTCCCTCGCTTTGGGTCTCCCTAATGCCTGCCATGCTCCTTCTAATATCACTGAGTGTATCTCTCTTTTGCACTTAGCACCAAACTCTACAGAAGCTAAGATATTCAAAGAGTCAAATTCCACAAGTGAACCAAGCAGTGCTCCAGATTCTAAAGTTCATGGGAGTGCGTCCACTGCCAACGAGAAAAGCGACGGTGGAACGAGCAAGAAACCGATTGGAGGAGCAGGGATGATTATGGAGATTCTGCTAGGGTTCTTCATTCTATCGCATCTCAATAATCTAAAGTTCATATAG

Coding sequence (CDS)

ATGGCTCTCTTTGTCTTCTGCAATCTGCTGCTTCTCCTGCTAGCTCCTTTTGCAGCCTCTAACATAGACCAAGATAGAACTGAGTGCTCAGACCAACTCATCGGCCTTGCCCCTTGCCTGCCTTATGTTAGCGGCGATGCCAAAACGCCTACCATTGATTGCTGCAGTGGGCTCAAGCAGGTGGTGCAGAAGAGTAAGAAGTGCCTCTGTGTTCTTATCAAGGATAAGGATGACCCTAATCTTGGCCTCAAAATCAATGGCTCCCTCGCTTTGGGTCTCCCTAATGCCTGCCATGCTCCTTCTAATATCACTGAGTGTATCTCTCTTTTGCACTTAGCACCAAACTCTACAGAAGCTAAGATATTCAAAGAGTCAAATTCCACAAGTGAACCAAGCAGTGCTCCAGATTCTAAAGTTCATGGGAGTGCGTCCACTGCCAACGAGAAAAGCGACGGTGGAACGAGCAAGAAACCGATTGGAGGAGCAGGGATGATTATGGAGATTCTGCTAGGGTTCTTCATTCTATCGCATCTCAATAATCTAAAGTTCATATAG

Protein sequence

MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQVVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAKIFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNNLKFI

Homology

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BLAST of Carg16971 vs. NCBI nr
Match: XP_022960972.1 (protein YLS3-like [Cucurbita moschata] >KAG6590243.1 Protein YLS3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023884.1 Protein YLS3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 361.3 bits (926), Expect = 4.9e-96
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60
           MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ
Sbjct: 1   MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60

Query: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120
           VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK
Sbjct: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120

Query: 121 IFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNN 180
           IFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNN
Sbjct: 121 IFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNN 180

Query: 181 LKFI 185
           LKFI
Sbjct: 181 LKFI 184

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BLAST of Carg16971 vs. NCBI nr
Match: XP_023515387.1 (protein YLS3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 356.3 bits (913), Expect = 1.6e-94
Identity = 181/184 (98.37%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60
           MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ
Sbjct: 1   MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60

Query: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120
           VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK
Sbjct: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120

Query: 121 IFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNN 180
           IFKESNSTSEPSSAPDSKVHGS+STANEKSDGGT KKPIGGAGMIMEILLGFFILSHLN+
Sbjct: 121 IFKESNSTSEPSSAPDSKVHGSSSTANEKSDGGTRKKPIGGAGMIMEILLGFFILSHLNS 180

Query: 181 LKFI 185
           LKFI
Sbjct: 181 LKFI 184

[Close]

BLAST of Carg16971 vs. NCBI nr
Match: XP_022988220.1 (protein YLS3-like [Cucurbita maxima])

HSP 1 Score: 353.2 bits (905), Expect = 1.3e-93
Identity = 179/184 (97.28%), Postives = 181/184 (98.37%), Query Frame = 0


Query: 1   MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60
           MALFVFCNLLLLLLAPFA SNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ
Sbjct: 1   MALFVFCNLLLLLLAPFAVSNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60

Query: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120
           VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK
Sbjct: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120

Query: 121 IFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNN 180
           IFKESNSTSEPSSAPDSKVHGS+STANEKSDGGT KKPIGG GMIMEILLGFFILSHLN+
Sbjct: 121 IFKESNSTSEPSSAPDSKVHGSSSTANEKSDGGTRKKPIGGVGMIMEILLGFFILSHLNS 180

Query: 181 LKFI 185
           LKFI
Sbjct: 181 LKFI 184

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BLAST of Carg16971 vs. NCBI nr
Match: XP_008451096.1 (PREDICTED: protein YLS3-like [Cucumis melo] >KAA0055642.1 protein YLS3-like [Cucumis melo var. makuwa] >TYK09896.1 protein YLS3-like [Cucumis melo var. makuwa])

HSP 1 Score: 302.8 bits (774), Expect = 2.1e-78
Identity = 158/184 (85.87%), Postives = 167/184 (90.76%), Query Frame = 0


Query: 1   MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60
           +A F F    LLLLA FAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ
Sbjct: 5   LATFSFICNFLLLLASFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 64

Query: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120
           VVQKSKKCLCVLIKDKDDPNLGLKINGSLAL LP+ACHAP+NITECISLLHL+PNSTEAK
Sbjct: 65  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALALPHACHAPANITECISLLHLSPNSTEAK 124

Query: 121 IFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNN 180
           IFKESN TSEPSS+PD+KVHGS+S ANEKSDGG  K+ IGG  MIM+ILLGFFILSHL N
Sbjct: 125 IFKESNPTSEPSSSPDTKVHGSSSAANEKSDGGMGKRWIGGTEMIMKILLGFFILSHL-N 184

Query: 181 LKFI 185
           L FI
Sbjct: 185 LMFI 187

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BLAST of Carg16971 vs. NCBI nr
Match: XP_038878589.1 (non-specific lipid transfer protein GPI-anchored 6-like [Benincasa hispida])

HSP 1 Score: 298.1 bits (762), Expect = 5.1e-77
Identity = 157/183 (85.79%), Postives = 164/183 (89.62%), Query Frame = 0


Query: 2   ALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQV 61
           A  +FCN  LLLLA FAASNIDQDRTECSDQL GLAPCLPYVSGDAKTPTIDCCSGLKQV
Sbjct: 7   AFSLFCN-FLLLLASFAASNIDQDRTECSDQLTGLAPCLPYVSGDAKTPTIDCCSGLKQV 66

Query: 62  VQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAKI 121
           VQKSKKCLCVLIKDKDDPNLGLKINGSLAL LPN CHAP+NITECISLLHL+PNSTEAKI
Sbjct: 67  VQKSKKCLCVLIKDKDDPNLGLKINGSLALALPNDCHAPANITECISLLHLSPNSTEAKI 126

Query: 122 FKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNNL 181
           FKESN TS PSS+PD+KVHGS+STANEKSDGG  K+ IG   MIM ILLGFFILSHL NL
Sbjct: 127 FKESNPTSGPSSSPDTKVHGSSSTANEKSDGGMGKRWIGSTEMIMGILLGFFILSHL-NL 186

Query: 182 KFI 185
            FI
Sbjct: 187 MFI 187

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BLAST of Carg16971 vs. ExPASy Swiss-Prot
Match: O64865 (Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3702 GN=LTPG14 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.1e-34
Identity = 81/187 (43.32%), Postives = 121/187 (64.71%), Query Frame = 0


Query: 9   LLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQVVQKSKKC 68
           L+++ +   AA +  +D+ EC++QL+G+A CLPYV G AK+PT DCCSGLKQV+  +KKC
Sbjct: 15  LIVVAMVVAAADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNSNKKC 74

Query: 69  LCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAKIF----KE 128
           LCV+I+D++DP+LGL+IN SLAL LP+ CHA +++T+C +LLHL PNS +A++F    K 
Sbjct: 75  LCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQVFYQLAKG 134

Query: 129 SNSTSEPSSAPDSKVHG--SASTANEKSDGGTSKKPIGGAG-----------MIMEILLG 179
            N T  P+SAP     G  S S  +   DG  S +     G           + +E++  
Sbjct: 135 LNKTG-PASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRNHAQSFYKQWLGLEVVFH 194

[Close]

BLAST of Carg16971 vs. ExPASy Swiss-Prot
Match: F4I082 (Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=3702 GN=LTPG6 PE=2 SV=2)

HSP 1 Score: 140.2 bits (352), Expect = 2.3e-32
Identity = 81/166 (48.80%), Postives = 107/166 (64.46%), Query Frame = 0


Query: 3   LFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQVV 62
           LF+   +  +LL  F  S++ QDR EC++QLI L+ C+PYV GDAK PT DCC+G  QV+
Sbjct: 8   LFITIVITSMLLG-FGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVI 67

Query: 63  QKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPS-NITECISLLHLAPNSTEAKI 122
           +KS+KC+C+L++DKDDP LG+KIN +LA  LP+ACH  + NIT+CIS+LHL  NST AK 
Sbjct: 68  RKSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKE 127

Query: 123 FK-------ESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIG 161
           F+         NSTS      D    G A     KS+G   K  +G
Sbjct: 128 FENLGRIEDNYNSTSPTQIHKDGTGGGKAEPV--KSNGWKEKSWLG 170

[Close]

BLAST of Carg16971 vs. ExPASy Swiss-Prot
Match: O64864 (Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3702 GN=LTPG13 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 8.9e-32
Identity = 75/179 (41.90%), Postives = 113/179 (63.13%), Query Frame = 0


Query: 9   LLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQVVQKSKKC 68
           +++ ++   A ++  +D+ EC+ QL+G+A CLPYV G AK+PT DCCSGLKQV+    KC
Sbjct: 16  IMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSDMKC 75

Query: 69  LCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAKIF------ 128
           LC++I++++DP+LGL++N SLAL LP+ CHA ++IT+C +LLHL PNS +A++F      
Sbjct: 76  LCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDPNSPDAQVFYQLAKG 135

Query: 129 -KESNSTSEP---SSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSH 178
             E+ S S P   +S P S      S+A   S   TS      A    +  LG  +++H
Sbjct: 136 LNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQSFSKQWLGLEVVAH 194

[Close]

BLAST of Carg16971 vs. ExPASy Swiss-Prot
Match: Q9M2G1 (Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3702 GN=LTPG22 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.4e-24
Identity = 58/143 (40.56%), Postives = 83/143 (58.04%), Query Frame = 0


Query: 20  SNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQVVQK--SKKCLCVLIKDKD 79
           S + QD   C D +  L  CLP+V+  AK P   CCS LK  + K  ++KCLC L+KD+D
Sbjct: 29  SELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKIDKGQTRKCLCTLVKDRD 88

Query: 80  DPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAKIFKESNSTSEPSSAPDS 139
           DP LG K++ + A+ LP+ACH P+NI++C  LLHL P+S  ++IFK+   +S       S
Sbjct: 89  DPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDSPASQIFKQFTESS-------S 148

Query: 140 KVHGSASTANEKSDGGTSKKPIG 161
           +  G  + +   S  G   K  G
Sbjct: 149 QTVGHKAVSTSSSIKGRDNKQFG 164

[Close]

BLAST of Carg16971 vs. ExPASy Swiss-Prot
Match: Q9C9B1 (Non-specific lipid transfer protein GPI-anchored 10 OS=Arabidopsis thaliana OX=3702 GN=LTPG10 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.1e-10
Identity = 45/151 (29.80%), Postives = 69/151 (45.70%), Query Frame = 0


Query: 9   LLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQVVQKSKKC 68
           LL+ L A F    + Q    C+ +L+ LAPC P+V G A+ P   CC  L Q+  +   C
Sbjct: 10  LLISLSAFFLRMVLAQVPATCASRLLSLAPCGPFVQGFAQLPAQPCCDSLNQIYSQEATC 69

Query: 69  LCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAKIFKESNST 128
           LC+ + +    +    IN +LAL LP  C+ P+N + C S               E+ S 
Sbjct: 70  LCLFLNNTSTLSPAFPINQTLALQLPPLCNIPANSSTCSS-----------SFPGEAPSD 129

Query: 129 SEPSSAPDSKVHGSASTANEKSDGGTSKKPI 160
           S   + P S   GS  +   K++   +  P+
Sbjct: 130 SSSVAPPPSSSTGSQISQGAKNNSRVAATPV 149

[Close]

BLAST of Carg16971 vs. ExPASy TrEMBL
Match: A0A6J1H8W0 (protein YLS3-like OS=Cucurbita moschata OX=3662 GN=LOC111461612 PE=3 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 2.4e-96
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60
           MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ
Sbjct: 1   MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60

Query: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120
           VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK
Sbjct: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120

Query: 121 IFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNN 180
           IFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNN
Sbjct: 121 IFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNN 180

Query: 181 LKFI 185
           LKFI
Sbjct: 181 LKFI 184

[Close]

BLAST of Carg16971 vs. ExPASy TrEMBL
Match: A0A6J1JIZ5 (protein YLS3-like OS=Cucurbita maxima OX=3661 GN=LOC111485523 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 6.5e-94
Identity = 179/184 (97.28%), Postives = 181/184 (98.37%), Query Frame = 0


Query: 1   MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60
           MALFVFCNLLLLLLAPFA SNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ
Sbjct: 1   MALFVFCNLLLLLLAPFAVSNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60

Query: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120
           VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK
Sbjct: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120

Query: 121 IFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNN 180
           IFKESNSTSEPSSAPDSKVHGS+STANEKSDGGT KKPIGG GMIMEILLGFFILSHLN+
Sbjct: 121 IFKESNSTSEPSSAPDSKVHGSSSTANEKSDGGTRKKPIGGVGMIMEILLGFFILSHLNS 180

Query: 181 LKFI 185
           LKFI
Sbjct: 181 LKFI 184

[Close]

BLAST of Carg16971 vs. ExPASy TrEMBL
Match: A0A5A7UIG8 (Protein YLS3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00060 PE=3 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 1.0e-78
Identity = 158/184 (85.87%), Postives = 167/184 (90.76%), Query Frame = 0


Query: 1   MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60
           +A F F    LLLLA FAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ
Sbjct: 5   LATFSFICNFLLLLASFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 64

Query: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120
           VVQKSKKCLCVLIKDKDDPNLGLKINGSLAL LP+ACHAP+NITECISLLHL+PNSTEAK
Sbjct: 65  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALALPHACHAPANITECISLLHLSPNSTEAK 124

Query: 121 IFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNN 180
           IFKESN TSEPSS+PD+KVHGS+S ANEKSDGG  K+ IGG  MIM+ILLGFFILSHL N
Sbjct: 125 IFKESNPTSEPSSSPDTKVHGSSSAANEKSDGGMGKRWIGGTEMIMKILLGFFILSHL-N 184

Query: 181 LKFI 185
           L FI
Sbjct: 185 LMFI 187

[Close]

BLAST of Carg16971 vs. ExPASy TrEMBL
Match: A0A1S3BRH1 (protein YLS3-like OS=Cucumis melo OX=3656 GN=LOC103492476 PE=3 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 1.0e-78
Identity = 158/184 (85.87%), Postives = 167/184 (90.76%), Query Frame = 0


Query: 1   MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60
           +A F F    LLLLA FAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ
Sbjct: 5   LATFSFICNFLLLLASFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 64

Query: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120
           VVQKSKKCLCVLIKDKDDPNLGLKINGSLAL LP+ACHAP+NITECISLLHL+PNSTEAK
Sbjct: 65  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALALPHACHAPANITECISLLHLSPNSTEAK 124

Query: 121 IFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNN 180
           IFKESN TSEPSS+PD+KVHGS+S ANEKSDGG  K+ IGG  MIM+ILLGFFILSHL N
Sbjct: 125 IFKESNPTSEPSSSPDTKVHGSSSAANEKSDGGMGKRWIGGTEMIMKILLGFFILSHL-N 184

Query: 181 LKFI 185
           L FI
Sbjct: 185 LMFI 187

[Close]

BLAST of Carg16971 vs. ExPASy TrEMBL
Match: A0A0A0LX07 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G602030 PE=4 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 1.0e-75
Identity = 152/184 (82.61%), Postives = 166/184 (90.22%), Query Frame = 0


Query: 1   MALFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQ 60
           ++ F F    LLLLA F+AS+IDQDRT+CSDQLIGLA CLPYVSGDAKTPTIDCCSGLKQ
Sbjct: 5   LSTFSFICNFLLLLASFSASDIDQDRTDCSDQLIGLAQCLPYVSGDAKTPTIDCCSGLKQ 64

Query: 61  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAK 120
           VVQKSKKCLCVLIKDKDDPNLGLKINGSLAL LP+ACHAP+NITECISLLHL+PNSTEAK
Sbjct: 65  VVQKSKKCLCVLIKDKDDPNLGLKINGSLALALPHACHAPANITECISLLHLSPNSTEAK 124

Query: 121 IFKESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSHLNN 180
           IFKESN TSEPSS+PD+KVHGS+S ANEKSDGG  K+ IGG  MI++ILLGFFILSHL N
Sbjct: 125 IFKESNPTSEPSSSPDTKVHGSSSVANEKSDGGMGKRWIGGTEMIVKILLGFFILSHL-N 184

Query: 181 LKFI 185
           L FI
Sbjct: 185 LMFI 187

[Close]

BLAST of Carg16971 vs. TAIR 10
Match: AT2G44300.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 147.9 bits (372), Expect = 8.0e-36
Identity = 81/187 (43.32%), Postives = 121/187 (64.71%), Query Frame = 0


Query: 9   LLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQVVQKSKKC 68
           L+++ +   AA +  +D+ EC++QL+G+A CLPYV G AK+PT DCCSGLKQV+  +KKC
Sbjct: 15  LIVVAMVVAAADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNSNKKC 74

Query: 69  LCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAKIF----KE 128
           LCV+I+D++DP+LGL+IN SLAL LP+ CHA +++T+C +LLHL PNS +A++F    K 
Sbjct: 75  LCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQVFYQLAKG 134

Query: 129 SNSTSEPSSAPDSKVHG--SASTANEKSDGGTSKKPIGGAG-----------MIMEILLG 179
            N T  P+SAP     G  S S  +   DG  S +     G           + +E++  
Sbjct: 135 LNKTG-PASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRNHAQSFYKQWLGLEVVFH 194

[Close]

BLAST of Carg16971 vs. TAIR 10
Match: AT1G55260.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 141.0 bits (354), Expect = 9.8e-34
Identity = 76/170 (44.71%), Postives = 111/170 (65.29%), Query Frame = 0


Query: 3   LFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQVV 62
           LF+   +  +LL  F  S++ QDR EC++QLI L+ C+PYV GDAK PT DCC+G  QV+
Sbjct: 51  LFITIVITSMLLG-FGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVI 110

Query: 63  QKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPS-NITECISLLHLAPNSTEAKI 122
           +KS+KC+C+L++DKDDP LG+KIN +LA  LP+ACH  + NIT+CIS+LHL  NST AK 
Sbjct: 111 RKSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKE 170

Query: 123 FKESNSTSEP-SSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILL 171
           F+      +  +S   +++H     A      G  +K   G  +++ +L+
Sbjct: 171 FENLGRIEDNYNSTSPTQIHKGGGKAEPVKSNGWKEKSWLGVELLIYLLV 219

[Close]

BLAST of Carg16971 vs. TAIR 10
Match: AT1G55260.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 140.2 bits (352), Expect = 1.7e-33
Identity = 81/166 (48.80%), Postives = 107/166 (64.46%), Query Frame = 0


Query: 3   LFVFCNLLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQVV 62
           LF+   +  +LL  F  S++ QDR EC++QLI L+ C+PYV GDAK PT DCC+G  QV+
Sbjct: 51  LFITIVITSMLLG-FGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVI 110

Query: 63  QKSKKCLCVLIKDKDDPNLGLKINGSLALGLPNACHAPS-NITECISLLHLAPNSTEAKI 122
           +KS+KC+C+L++DKDDP LG+KIN +LA  LP+ACH  + NIT+CIS+LHL  NST AK 
Sbjct: 111 RKSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKE 170

Query: 123 FK-------ESNSTSEPSSAPDSKVHGSASTANEKSDGGTSKKPIG 161
           F+         NSTS      D    G A     KS+G   K  +G
Sbjct: 171 FENLGRIEDNYNSTSPTQIHKDGTGGGKAEPV--KSNGWKEKSWLG 213

[Close]

BLAST of Carg16971 vs. TAIR 10
Match: AT2G44290.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 6.3e-33
Identity = 75/179 (41.90%), Postives = 113/179 (63.13%), Query Frame = 0


Query: 9   LLLLLLAPFAASNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQVVQKSKKC 68
           +++ ++   A ++  +D+ EC+ QL+G+A CLPYV G AK+PT DCCSGLKQV+    KC
Sbjct: 16  IMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSDMKC 75

Query: 69  LCVLIKDKDDPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAKIF------ 128
           LC++I++++DP+LGL++N SLAL LP+ CHA ++IT+C +LLHL PNS +A++F      
Sbjct: 76  LCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDPNSPDAQVFYQLAKG 135

Query: 129 -KESNSTSEP---SSAPDSKVHGSASTANEKSDGGTSKKPIGGAGMIMEILLGFFILSH 178
             E+ S S P   +S P S      S+A   S   TS      A    +  LG  +++H
Sbjct: 136 LNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQSFSKQWLGLEVVAH 194

[Close]

BLAST of Carg16971 vs. TAIR 10
Match: AT3G58550.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 113.6 bits (283), Expect = 1.7e-25
Identity = 58/143 (40.56%), Postives = 83/143 (58.04%), Query Frame = 0


Query: 20  SNIDQDRTECSDQLIGLAPCLPYVSGDAKTPTIDCCSGLKQVVQK--SKKCLCVLIKDKD 79
           S + QD   C D +  L  CLP+V+  AK P   CCS LK  + K  ++KCLC L+KD+D
Sbjct: 29  SELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKIDKGQTRKCLCTLVKDRD 88

Query: 80  DPNLGLKINGSLALGLPNACHAPSNITECISLLHLAPNSTEAKIFKESNSTSEPSSAPDS 139
           DP LG K++ + A+ LP+ACH P+NI++C  LLHL P+S  ++IFK+   +S       S
Sbjct: 89  DPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDSPASQIFKQFTESS-------S 148

Query: 140 KVHGSASTANEKSDGGTSKKPIG 161
           +  G  + +   S  G   K  G
Sbjct: 149 QTVGHKAVSTSSSIKGRDNKQFG 164

The following BLAST results are available for this feature:

BLAST of Carg16971 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022960972.1	4.9e-96	100.00	protein YLS3-like [Cucurbita moschata] >KAG6590243.1 Protein YLS3, partial [Cucu...	[more]
XP_023515387.1	1.6e-94	98.37	protein YLS3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022988220.1	1.3e-93	97.28	protein YLS3-like [Cucurbita maxima]	[more]
XP_008451096.1	2.1e-78	85.87	PREDICTED: protein YLS3-like [Cucumis melo] >KAA0055642.1 protein YLS3-like [Cuc...	[more]
XP_038878589.1	5.1e-77	85.79	non-specific lipid transfer protein GPI-anchored 6-like [Benincasa hispida]	[more]

BLAST of Carg16971 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64865	1.1e-34	43.32	Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3...	[more]
F4I082	2.3e-32	48.80	Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=37...	[more]
O64864	8.9e-32	41.90	Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3...	[more]
Q9M2G1	2.4e-24	40.56	Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3...	[more]
Q9C9B1	1.1e-10	29.80	Non-specific lipid transfer protein GPI-anchored 10 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Carg16971 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H8W0	2.4e-96	100.00	protein YLS3-like OS=Cucurbita moschata OX=3662 GN=LOC111461612 PE=3 SV=1	[more]
A0A6J1JIZ5	6.5e-94	97.28	protein YLS3-like OS=Cucurbita maxima OX=3661 GN=LOC111485523 PE=3 SV=1	[more]
A0A5A7UIG8	1.0e-78	85.87	Protein YLS3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G000...	[more]
A0A1S3BRH1	1.0e-78	85.87	protein YLS3-like OS=Cucumis melo OX=3656 GN=LOC103492476 PE=3 SV=1	[more]
A0A0A0LX07	1.0e-75	82.61	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G602030 PE=4 SV...	[more]

BLAST of Carg16971 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44300.1	8.0e-36	43.32	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G55260.2	9.8e-34	44.71	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G55260.1	1.7e-33	48.80	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G44290.1	6.3e-33	41.90	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT3G58550.1	1.7e-25	40.56	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000528	Plant non-specific lipid-transfer protein/Par allergen	PRINTS	PR00382	LIPIDTRNSFER	coord: 28..44 score: 33.12 coord: 84..101 score: 29.15 coord: 49..63 score: 30.36
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 29..106 e-value: 4.4E-7 score: 39.5
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 11..106 e-value: 1.0E-13 score: 51.2
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 26..106 e-value: 6.6E-14 score: 53.5
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 27..107
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 121..152
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 121..158
None	No IPR available	PANTHER	PTHR33044:SF194	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN	coord: 7..170
None	No IPR available	CDD	cd00010	AAI_LTSS	coord: 36..98 e-value: 1.76167E-18 score: 73.2298
IPR043325	Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) Protein	PANTHER	PTHR33044	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED	coord: 7..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16971-RA	Carg16971-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006869	lipid transport
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0008289	lipid binding