Carg16548 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16548
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Embryo sac development arrest protein
Location	Carg_Chr14: 13131518 .. 13131889 (-)
RNA-Seq Expression	Carg16548
Synteny	Carg16548

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTTCCATTTCCACAGCCGTGGGCTGCTACCGGCTGGGCCGTCCAGAAAAAGAAAGGAGCCGGCGGCTCTTAAAGCCAGTGAGCCACCAGTTTCTTCGAATCGGCTATTAGCCGGGTACATGGCTTATGAGTTTCTGACAAAAGGGACCATCTTGGGCCGGGAGTTTAACCCGGGTCGAGCCGAAGCAATCCCATCCGCCGCCGCTCAGTGGAAGAAGTCGAAGTCGGAAGCGGCGCCGCCAGAGATCTTGAAGAAAGAGCATCAGATTCAGAGCTACACTGAGGTGGCGACCATTTTGAAGACTACTGGGGGTCATATACCCGAAATCGTGAACCCGACCCAACTGGGTCGGTGGCTTCAAAAGTAA

mRNA sequence

ATGAGTTTCCATTTCCACAGCCGTGGGCTGCTACCGGCTGGGCCGTCCAGAAAAAGAAAGGAGCCGGCGGCTCTTAAAGCCAGTGAGCCACCAGTTTCTTCGAATCGGCTATTAGCCGGGTACATGGCTTATGAGTTTCTGACAAAAGGGACCATCTTGGGCCGGGAGTTTAACCCGGGTCGAGCCGAAGCAATCCCATCCGCCGCCGCTCAGTGGAAGAAGTCGAAGTCGGAAGCGGCGCCGCCAGAGATCTTGAAGAAAGAGCATCAGATTCAGAGCTACACTGAGGTGGCGACCATTTTGAAGACTACTGGGGGTCATATACCCGAAATCGTGAACCCGACCCAACTGGGTCGGTGGCTTCAAAAGTAA

Coding sequence (CDS)

ATGAGTTTCCATTTCCACAGCCGTGGGCTGCTACCGGCTGGGCCGTCCAGAAAAAGAAAGGAGCCGGCGGCTCTTAAAGCCAGTGAGCCACCAGTTTCTTCGAATCGGCTATTAGCCGGGTACATGGCTTATGAGTTTCTGACAAAAGGGACCATCTTGGGCCGGGAGTTTAACCCGGGTCGAGCCGAAGCAATCCCATCCGCCGCCGCTCAGTGGAAGAAGTCGAAGTCGGAAGCGGCGCCGCCAGAGATCTTGAAGAAAGAGCATCAGATTCAGAGCTACACTGAGGTGGCGACCATTTTGAAGACTACTGGGGGTCATATACCCGAAATCGTGAACCCGACCCAACTGGGTCGGTGGCTTCAAAAGTAA

Protein sequence

MSFHFHSRGLLPAGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPGRAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRWLQK

Homology

[Close]

BLAST of Carg16548 vs. NCBI nr
Match: XP_022924296.1 (uncharacterized protein LOC111431827 [Cucurbita moschata] >KAG6582401.1 hypothetical protein SDJN03_22403, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018804.1 hypothetical protein SDJN02_20677, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 247.7 bits (631), Expect = 5.3e-62
Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 0


Query: 1   MSFHFHSRGLLPAGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG 60
           MSFHFHSRGLLPAGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG
Sbjct: 1   MSFHFHSRGLLPAGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG 60

Query: 61  RAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRW 120
           RAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRW
Sbjct: 61  RAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRW 120

Query: 121 LQK 124
           LQK
Sbjct: 121 LQK 123

[Close]

BLAST of Carg16548 vs. NCBI nr
Match: XP_023526927.1 (uncharacterized protein LOC111790299 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 244.6 bits (623), Expect = 4.5e-61
Identity = 122/123 (99.19%), Postives = 122/123 (99.19%), Query Frame = 0


Query: 1   MSFHFHSRGLLPAGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG 60
           MSFHFHSRGLLPAGPSRKRKEPAALKASEP VSSNRLLAGYMAYEFLTKGTILGREFNPG
Sbjct: 1   MSFHFHSRGLLPAGPSRKRKEPAALKASEPSVSSNRLLAGYMAYEFLTKGTILGREFNPG 60

Query: 61  RAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRW 120
           RAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRW
Sbjct: 61  RAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRW 120

Query: 121 LQK 124
           LQK
Sbjct: 121 LQK 123

[Close]

BLAST of Carg16548 vs. NCBI nr
Match: XP_022979583.1 (uncharacterized protein LOC111479265 [Cucurbita maxima])

HSP 1 Score: 239.6 bits (610), Expect = 1.4e-59
Identity = 119/123 (96.75%), Postives = 120/123 (97.56%), Query Frame = 0


Query: 1   MSFHFHSRGLLPAGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG 60
           MSFHFH RGLLP GPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG
Sbjct: 1   MSFHFHIRGLLPPGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG 60

Query: 61  RAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRW 120
           +AEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTG HIPEIVNPTQLGRW
Sbjct: 61  QAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGAHIPEIVNPTQLGRW 120

Query: 121 LQK 124
           LQK
Sbjct: 121 LQK 123

[Close]

BLAST of Carg16548 vs. NCBI nr
Match: KGN56835.1 (hypothetical protein Csa_009887 [Cucumis sativus])

HSP 1 Score: 194.9 bits (494), Expect = 4.1e-46
Identity = 102/130 (78.46%), Postives = 109/130 (83.85%), Query Frame = 0


Query: 1   MSFHFHSRGLLPAGPSRKRKE---PAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREF 60
           MSFHFHSRGLL AGPS+KRKE   P+A KA EP VSSNRLLAGYMAYEFLTKGT+ GR+F
Sbjct: 1   MSFHFHSRGLLSAGPSKKRKEPSTPSASKAGEPSVSSNRLLAGYMAYEFLTKGTLFGRKF 60

Query: 61  NPGRAEAIPSAAA----QWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVN 120
           +P R EA PSAA+    QWKK KS+AAPPEILKKEHQIQSY EVA ILKTTG HI  IVN
Sbjct: 61  DPPRDEATPSAASAAVIQWKKPKSDAAPPEILKKEHQIQSYAEVANILKTTGSHISGIVN 120

Query: 121 PTQLGRWLQK 124
           PTQLGRWLQK
Sbjct: 121 PTQLGRWLQK 130

[Close]

BLAST of Carg16548 vs. NCBI nr
Match: XP_023539410.1 (uncharacterized protein LOC111800062 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 189.1 bits (479), Expect = 2.2e-44
Identity = 99/126 (78.57%), Postives = 107/126 (84.92%), Query Frame = 0


Query: 1   MSFHFHSRGLLPAGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG 60
           MSFH HSRGLL AGPSRKRKEP+ALKA E PV+SNRLLAGYMAYEFLTKGTILGR+F+P 
Sbjct: 1   MSFHCHSRGLLSAGPSRKRKEPSALKAGEQPVTSNRLLAGYMAYEFLTKGTILGRKFDPD 60

Query: 61  RAEAIP---SAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQL 120
           RAEA P    AAAQ+KK K+EAAPPEI+KKEHQIQSYT+VATILKT    I  IVNPTQL
Sbjct: 61  RAEASPFPTIAAAQYKKPKTEAAPPEIIKKEHQIQSYTDVATILKTDKAQISGIVNPTQL 120

Query: 121 GRWLQK 124
            RWL K
Sbjct: 121 ARWLSK 126

[Close]

BLAST of Carg16548 vs. ExPASy TrEMBL
Match: A0A6J1E960 (uncharacterized protein LOC111431827 OS=Cucurbita moschata OX=3662 GN=LOC111431827 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.6e-62
Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 0


Query: 1   MSFHFHSRGLLPAGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG 60
           MSFHFHSRGLLPAGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG
Sbjct: 1   MSFHFHSRGLLPAGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG 60

Query: 61  RAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRW 120
           RAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRW
Sbjct: 61  RAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRW 120

Query: 121 LQK 124
           LQK
Sbjct: 121 LQK 123

[Close]

BLAST of Carg16548 vs. ExPASy TrEMBL
Match: A0A6J1IWN8 (uncharacterized protein LOC111479265 OS=Cucurbita maxima OX=3661 GN=LOC111479265 PE=4 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 7.0e-60
Identity = 119/123 (96.75%), Postives = 120/123 (97.56%), Query Frame = 0


Query: 1   MSFHFHSRGLLPAGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG 60
           MSFHFH RGLLP GPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG
Sbjct: 1   MSFHFHIRGLLPPGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPG 60

Query: 61  RAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRW 120
           +AEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTG HIPEIVNPTQLGRW
Sbjct: 61  QAEAIPSAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGAHIPEIVNPTQLGRW 120

Query: 121 LQK 124
           LQK
Sbjct: 121 LQK 123

[Close]

BLAST of Carg16548 vs. ExPASy TrEMBL
Match: A0A0A0L4C1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G134830 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.0e-46
Identity = 102/130 (78.46%), Postives = 109/130 (83.85%), Query Frame = 0


Query: 1   MSFHFHSRGLLPAGPSRKRKE---PAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREF 60
           MSFHFHSRGLL AGPS+KRKE   P+A KA EP VSSNRLLAGYMAYEFLTKGT+ GR+F
Sbjct: 1   MSFHFHSRGLLSAGPSKKRKEPSTPSASKAGEPSVSSNRLLAGYMAYEFLTKGTLFGRKF 60

Query: 61  NPGRAEAIPSAAA----QWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVN 120
           +P R EA PSAA+    QWKK KS+AAPPEILKKEHQIQSY EVA ILKTTG HI  IVN
Sbjct: 61  DPPRDEATPSAASAAVIQWKKPKSDAAPPEILKKEHQIQSYAEVANILKTTGSHISGIVN 120

Query: 121 PTQLGRWLQK 124
           PTQLGRWLQK
Sbjct: 121 PTQLGRWLQK 130

[Close]

BLAST of Carg16548 vs. ExPASy TrEMBL
Match: A0A5A7U1L2 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001830 PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.0e-43
Identity = 100/131 (76.34%), Postives = 106/131 (80.92%), Query Frame = 0


Query: 1   MSFHFHSRGLLPAGPSRKRKEPAA----LKASEPPVSSNRLLAGYMAYEFLTKGTILGRE 60
           MSFHFHSRGLL AGPS+KRKEP+      KA EP V SNRLLAGYMAYEFLTKGT+ GR+
Sbjct: 1   MSFHFHSRGLLSAGPSKKRKEPSTPAPPNKAGEPSVLSNRLLAGYMAYEFLTKGTLFGRK 60

Query: 61  FNPGRAEAIPSAAA--QWKKSKSEAAPPEILKKEH--QIQSYTEVATILKTTGGHIPEIV 120
           F+P R EA PS+AA  QWKK K EAAPPEILKKEH  QIQSY EVA ILKTTG HI  IV
Sbjct: 61  FDPTRDEATPSSAAVSQWKKPKFEAAPPEILKKEHQNQIQSYAEVANILKTTGSHISGIV 120

Query: 121 NPTQLGRWLQK 124
           NPTQLGRWLQK
Sbjct: 121 NPTQLGRWLQK 131

[Close]

BLAST of Carg16548 vs. ExPASy TrEMBL
Match: A0A1S3AWH5 (uncharacterized protein LOC103483571 OS=Cucumis melo OX=3656 GN=LOC103483571 PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.0e-43
Identity = 100/131 (76.34%), Postives = 106/131 (80.92%), Query Frame = 0


Query: 1   MSFHFHSRGLLPAGPSRKRKEPAA----LKASEPPVSSNRLLAGYMAYEFLTKGTILGRE 60
           MSFHFHSRGLL AGPS+KRKEP+      KA EP V SNRLLAGYMAYEFLTKGT+ GR+
Sbjct: 1   MSFHFHSRGLLSAGPSKKRKEPSTPAPPNKAGEPSVLSNRLLAGYMAYEFLTKGTLFGRK 60

Query: 61  FNPGRAEAIPSAAA--QWKKSKSEAAPPEILKKEH--QIQSYTEVATILKTTGGHIPEIV 120
           F+P R EA PS+AA  QWKK K EAAPPEILKKEH  QIQSY EVA ILKTTG HI  IV
Sbjct: 61  FDPTRDEATPSSAAVSQWKKPKFEAAPPEILKKEHQNQIQSYAEVANILKTTGSHISGIV 120

Query: 121 NPTQLGRWLQK 124
           NPTQLGRWLQK
Sbjct: 121 NPTQLGRWLQK 131

[Close]

BLAST of Carg16548 vs. TAIR 10
Match: AT3G23440.1 (embryo sac development arrest 6 )

HSP 1 Score: 81.6 bits (200), Expect = 4.7e-16
Identity = 50/110 (45.45%), Postives = 69/110 (62.73%), Query Frame = 0


Query: 14  GPSRKRKEPAA-LKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPGRAEAIPSAAAQW 73
           G SRKRK+  + L+ +  P   N LLAGYMA+E+LT GT+LGR+   G AE  P  +   
Sbjct: 10  GASRKRKDTESDLREAATP---NWLLAGYMAHEYLTCGTMLGRKLYSGWAEVGPLVSPSP 69

Query: 74  KKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRWLQ 123
            +S+        +KK    QSY+EVA++ KT G H+P +VNPTQL +W+Q
Sbjct: 70  LQSRE-------VKKAR--QSYSEVASVFKTDGNHVPGVVNPTQLAKWIQ 107

[Close]

BLAST of Carg16548 vs. TAIR 10
Match: AT5G44060.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G04000.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 79.7 bits (195), Expect = 1.8e-15
Identity = 45/116 (38.79%), Postives = 68/116 (58.62%), Query Frame = 0


Query: 7   SRGLLPAGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPGRAEAIP 66
           ++ ++   P   R    A  A   PV SN+LLAGY+A+EFL  GT+ G  +NP +A+A P
Sbjct: 35  TKSIVKKTPIIPRPTTTATVALAEPVCSNQLLAGYLAHEFLNNGTLFGELWNPTKAQAGP 94

Query: 67  SAAAQWKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRWLQ 123
                    K++ +  +I   +H+ + Y EVA IL+  G H+P IVNP+QL R+L+
Sbjct: 95  LTTQSIDPRKNKPS-HDIEPSDHKRRRYVEVANILRVDGTHLPGIVNPSQLARFLK 149

[Close]

BLAST of Carg16548 vs. TAIR 10
Match: AT1G04000.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G44060.1); Has 62 Blast hits to 62 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 62; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 67.4 bits (163), Expect = 9.2e-12
Identity = 42/111 (37.84%), Postives = 64/111 (57.66%), Query Frame = 0


Query: 12  PAGPSRKRKEPAALKASEPPVSSNRLLAGYMAYEFLTKGTILGREFNPGRAEAIPSAAAQ 71
           P  P  K+   A   A+EP  S+  +LAGY+++E+LT+GT+ G ++N  RA+A  S    
Sbjct: 50  PVNPISKKSSTA---AAEPIGSNQLMLAGYLSHEYLTQGTLFGEQWNQARAQAESSKI-- 109

Query: 72  WKKSKSEAAPPEILKKEHQIQSYTEVATILKTTGGHIPEIVNPTQLGRWLQ 123
             K      P E  + E + + Y EVA +L++ G  +P IVNP QL R+L+
Sbjct: 110 --KPSHTVEPAE--ECEPKRKRYREVANLLRSDGAQLPGIVNPAQLARFLK 151

The following BLAST results are available for this feature:

BLAST of Carg16548 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022924296.1	5.3e-62	100.00	uncharacterized protein LOC111431827 [Cucurbita moschata] >KAG6582401.1 hypothet...	[more]
XP_023526927.1	4.5e-61	99.19	uncharacterized protein LOC111790299 [Cucurbita pepo subsp. pepo]	[more]
XP_022979583.1	1.4e-59	96.75	uncharacterized protein LOC111479265 [Cucurbita maxima]	[more]
KGN56835.1	4.1e-46	78.46	hypothetical protein Csa_009887 [Cucumis sativus]	[more]
XP_023539410.1	2.2e-44	78.57	uncharacterized protein LOC111800062 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg16548 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg16548 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E960	2.6e-62	100.00	uncharacterized protein LOC111431827 OS=Cucurbita moschata OX=3662 GN=LOC1114318...	[more]
A0A6J1IWN8	7.0e-60	96.75	uncharacterized protein LOC111479265 OS=Cucurbita maxima OX=3661 GN=LOC111479265...	[more]
A0A0A0L4C1	2.0e-46	78.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G134830 PE=4 SV=1	[more]
A0A5A7U1L2	2.0e-43	76.34	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3AWH5	2.0e-43	76.34	uncharacterized protein LOC103483571 OS=Cucumis melo OX=3656 GN=LOC103483571 PE=...	[more]

BLAST of Carg16548 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23440.1	4.7e-16	45.45	embryo sac development arrest 6	[more]
AT5G44060.1	1.8e-15	38.79	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G04000.1	9.2e-12	37.84	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..31
None	No IPR available	PANTHER	PTHR34657	EMBRYO SAC DEVELOPMENT ARREST 6	coord: 20..122

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16548-RA	Carg16548-RA	mRNA