Carg16490 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16490
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Asparagine--tRNA ligase
Location	Carg_Chr12: 7873942 .. 7876885 (+)
RNA-Seq Expression	Carg16490
Synteny	Carg16490

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTGAATTCAACTGCAGTGAAGATACACCGGGAACGGACCAATGGCAGAGCATTCCGCGCCACCCATTGACCAACTATCCGCCGTCGCCTTGAACGACGATGTTTCCACTTACAAGTCTCTCAAAGCCGAATTCTCCGATCGCGTTCCGATCCGTACAATCGTCTGCCGGCCAGACGGCGGCTCTGGACTCGCTGGCCATAAGGTTCGGGTTGGTGGTTGGGTTAAAACCGGAAGGAAGGCGGACAAGGACGCTTTTGCCTTTCTTGAGTTGAACGACGGCTCTTGCCCTCAGAATCTCCAAGTGATCATCGAAGCGGCTGTGGCGGATCTTGGCCAGATTGTCCCCACCGGAACCTGCGTTGTTGTCGATGGCGTTTTTAAGCTTCCTCCTCCGGATAAGCACCAGAAAATCGAGCTTCGTGCTGAGGCTGTCGTCCATGTTGGTCCGGTCGATCCTGCGCGGTATCCTTTGCCGAAGACGAAGCTCACTCTTGAGTTTCTTAGGGATTTCGTTCATCTCCGCCCTAGAACAAACACGGTAAAAGAAATTTAAATCGTTTGTCAAATTAAATTTGAAGTTAGTTATTGGATCGAATCGTTTCGATTGATCTTTTCACATTCTGCATTTCATTTCTTATGTAATTATATAACTTGGCATATACATGGTTTGATCCTCTCTAACGTCAATAGAAAGTTGCAATTTATAGAACATAATCACTTTTATTGATGAGTATGTGTTTTCTTCATTTGACGATTACAGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCATATGCCACTCACACATTTTTCAACAAGCATGGATTTCTGTATATTCACACGCCAATTGTCACCACAAGCGATTGTGAGGGAGCGGGTGAAATGTTTCAAGTTACCACTTTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGATTAAAAACCCACCTCCATCAGAAGCGGAAGTAGACGAAGCAAGAAAAATTGTCAAGGAGAAAGGGGAAGCTGTTTCAAACCTGAAGGCTTCTAAAGCAAGCAAGCAGGAAATCGGTGCTTCTGTTTCGGAACTGAAGAAGGCAAAGGAGAATGTTGCTAGGTTGGAAGAGAGAGCAAAACTTAAACCAGGCATTCCAGAGAAGGATGGGAAAGTTGATTATGGAAGAGATTTCTTTGCTCGGCAAGCGTTTTTGACTGTTTCGGGCCAGTTGCAAGTCGAAACTTATGCGTGTGCACTCAGTAGTGTATACACCTTTGGCCCGACCTTCCGTGCCGAAAATTCTCACACTTCAAGACATCTTGCAGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGTTTGTTACTATGCCAAAATTGTGTTCTTAGTTGTGTGTTTTTCTGCATACTCACGTATAACCATCATAAGACAATTCTGGCACTTAGCTTATAGTTGAGTTTATGTGTTCTAGGATGAGTTTATCATACTCATGGATGTGAAAAAACTAAGTTACTTTATAAGAAATTTAGGATCCTACTTGATATACGTTCCATGAGAACTGTAATTGAGAAGAAACTCGTCGAGGACGAAGTTTTAGTTGGCTGTCTCGCTGTGCTACTAAGCAATATGGCAAACAACAATGGATATCTATTAGGAATAAGCAATCAATGGAACTCTACAGTAGTTCAGTGCTCTTCTTCTAGAGATTTGATTGAATTTACCATATTTGACATATTTTCTTTAATTGGTTGAAGGATGATATGAATTGCGCTGAAGCATATGTGAGATTTCTGTGTCAATGGTTACTTGATAACTGCCTAGATGACATGAAATTTATGGCTGATAAGTACGACCGAACTTGTATCGATCGTCTCAAATTGGTCTCTTCAACCCCTTTTGTACGAACTTCGTATACGGAAGCTATTGAAATACTTGAGGAAGCCATAAAGAAGGGTAAGGAGTTTGAGAACAAAGTGGAATGGGGAATTGATTTAGCTTCCGAACATGAAAGGTCTGTAAACTCCCATGCTTTTCATTTACATTCCACTCGAACAATGGCTATAGTTCTCATTGTTTTTCTCTATCTCAGATACTTGACTGAGGTAGTATTTAAAAAGCCTGTCATCGTCTATAACTATCCAAAGGGAATCAAAGCCTTTTATATGAGGCTCAATGACGACTCGAAAACTGTAGCTGCAATGGATGTTCTTGTACCTAAGGTAGTTCACTTCGGTTCGATTTTACTTCTATAACTGTGATTAGTTTCACCCTTTTAATCCTTTTGTTAAAAATCAAACTTTTTCCTTGGATTGAAGGTTGGGGAGTTGATTGGTGGAAGTCAAAGGGAGGAGCGATTCGAGGTTATTCGCGACAGGTAGAAGAACAACTCATATCTCTCTTGTTGATTCTTGTTTCTTTTCTGAACCATGAAGTATGAATTATCATCTCCTGCAGGATTAAGGAGGTAGGTCTTCCACTTGAACCATATGAATGGTATCTTGATCTGAGACGATACGGAACAGTCAAGCACAGCGGTTTTGGTCTCGGCTTCGAGCGAATGCTTCTTTTTGCCACAGGCATTGACAACATCCGAGACGTTATCCCCTTCCCAAGATATCCAGGACGAGCGGACCTATGATATGATACATTTTGTAACACAAGGAAGGTCCTAGATTTCTTTACTAGCTGATGAAACTAAACTCCTTTGAACCAAAGGAGATTTGATTGTTTGTATTATATATATATTTTTTTTTTATCATTTTAGGGTTTAATTTATTCTCATAATTCTTGGATTTGTGGGTTGATTTGAAATGACATTCATCGGTTTCTTCATGTTTTCGATGTTTTGGTACTAACATTTTTTAATTTGTTTAATCATCTAATGCATCATACTATAAGATGCTTCCGATTATGCTCCTTATGTTGTCTGTTCTAAAAGTTTATTATAT

mRNA sequence

TTTTGAATTCAACTGCAGTGAAGATACACCGGGAACGGACCAATGGCAGAGCATTCCGCGCCACCCATTGACCAACTATCCGCCGTCGCCTTGAACGACGATGTTTCCACTTACAAGTCTCTCAAAGCCGAATTCTCCGATCGCGTTCCGATCCGTACAATCGTCTGCCGGCCAGACGGCGGCTCTGGACTCGCTGGCCATAAGGTTCGGGTTGGTGGTTGGGTTAAAACCGGAAGGAAGGCGGACAAGGACGCTTTTGCCTTTCTTGAGTTGAACGACGGCTCTTGCCCTCAGAATCTCCAAGTGATCATCGAAGCGGCTGTGGCGGATCTTGGCCAGATTGTCCCCACCGGAACCTGCGTTGTTGTCGATGGCGTTTTTAAGCTTCCTCCTCCGGATAAGCACCAGAAAATCGAGCTTCGTGCTGAGGCTGTCGTCCATGTTGGTCCGGTCGATCCTGCGCGGTATCCTTTGCCGAAGACGAAGCTCACTCTTGAGTTTCTTAGGGATTTCGTTCATCTCCGCCCTAGAACAAACACGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCATATGCCACTCACACATTTTTCAACAAGCATGGATTTCTGTATATTCACACGCCAATTGTCACCACAAGCGATTGTGAGGGAGCGGGTGAAATGTTTCAAGTTACCACTTTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGATTAAAAACCCACCTCCATCAGAAGCGGAAGTAGACGAAGCAAGAAAAATTGTCAAGGAGAAAGGGGAAGCTGTTTCAAACCTGAAGGCTTCTAAAGCAAGCAAGCAGGAAATCGGTGCTTCTGTTTCGGAACTGAAGAAGGCAAAGGAGAATGTTGCTAGGTTGGAAGAGAGAGCAAAACTTAAACCAGGCATTCCAGAGAAGGATGGGAAAGTTGATTATGGAAGAGATTTCTTTGCTCGGCAAGCGTTTTTGACTGTTTCGGGCCAGTTGCAAGTCGAAACTTATGCGTGTGCACTCAGTAGTGTATACACCTTTGGCCCGACCTTCCGTGCCGAAAATTCTCACACTTCAAGACATCTTGCAGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGATGATATGAATTGCGCTGAAGCATATGTGAGATTTCTGTGTCAATGGTTACTTGATAACTGCCTAGATGACATGAAATTTATGGCTGATAAGTACGACCGAACTTGTATCGATCGTCTCAAATTGGTCTCTTCAACCCCTTTTGTACGAACTTCGTATACGGAAGCTATTGAAATACTTGAGGAAGCCATAAAGAAGGGTAAGGAGTTTGAGAACAAAGTGGAATGGGGAATTGATTTAGCTTCCGAACATGAAAGATACTTGACTGAGGTAGTATTTAAAAAGCCTGTCATCGTCTATAACTATCCAAAGGGAATCAAAGCCTTTTATATGAGGCTCAATGACGACTCGAAAACTGTAGCTGCAATGGATGTTCTTGTACCTAAGGTTGGGGAGTTGATTGGTGGAAGTCAAAGGGAGGAGCGATTCGAGGTTATTCGCGACAGGATTAAGGAGGTAGGTCTTCCACTTGAACCATATGAATGGTATCTTGATCTGAGACGATACGGAACAGTCAAGCACAGCGGTTTTGGTCTCGGCTTCGAGCGAATGCTTCTTTTTGCCACAGGCATTGACAACATCCGAGACGTTATCCCCTTCCCAAGATATCCAGGACGAGCGGACCTATGATATGATACATTTTGTAACACAAGGAAGGTCCTAGATTTCTTTACTAGCTGATGAAACTAAACTCCTTTGAACCAAAGGAGATTTGATTGTTTGTATTATATATATATTTTTTTTTTATCATTTTAGGGTTTAATTTATTCTCATAATTCTTGGATTTGTGGGTTGATTTGAAATGACATTCATCGGTTTCTTCATGTTTTCGATGTTTTGGTACTAACATTTTTTAATTTGTTTAATCATCTAATGCATCATACTATAAGATGCTTCCGATTATGCTCCTTATGTTGTCTGTTCTAAAAGTTTATTATAT

Coding sequence (CDS)

ATGGCAGAGCATTCCGCGCCACCCATTGACCAACTATCCGCCGTCGCCTTGAACGACGATGTTTCCACTTACAAGTCTCTCAAAGCCGAATTCTCCGATCGCGTTCCGATCCGTACAATCGTCTGCCGGCCAGACGGCGGCTCTGGACTCGCTGGCCATAAGGTTCGGGTTGGTGGTTGGGTTAAAACCGGAAGGAAGGCGGACAAGGACGCTTTTGCCTTTCTTGAGTTGAACGACGGCTCTTGCCCTCAGAATCTCCAAGTGATCATCGAAGCGGCTGTGGCGGATCTTGGCCAGATTGTCCCCACCGGAACCTGCGTTGTTGTCGATGGCGTTTTTAAGCTTCCTCCTCCGGATAAGCACCAGAAAATCGAGCTTCGTGCTGAGGCTGTCGTCCATGTTGGTCCGGTCGATCCTGCGCGGTATCCTTTGCCGAAGACGAAGCTCACTCTTGAGTTTCTTAGGGATTTCGTTCATCTCCGCCCTAGAACAAACACGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCATATGCCACTCACACATTTTTCAACAAGCATGGATTTCTGTATATTCACACGCCAATTGTCACCACAAGCGATTGTGAGGGAGCGGGTGAAATGTTTCAAGTTACCACTTTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGATTAAAAACCCACCTCCATCAGAAGCGGAAGTAGACGAAGCAAGAAAAATTGTCAAGGAGAAAGGGGAAGCTGTTTCAAACCTGAAGGCTTCTAAAGCAAGCAAGCAGGAAATCGGTGCTTCTGTTTCGGAACTGAAGAAGGCAAAGGAGAATGTTGCTAGGTTGGAAGAGAGAGCAAAACTTAAACCAGGCATTCCAGAGAAGGATGGGAAAGTTGATTATGGAAGAGATTTCTTTGCTCGGCAAGCGTTTTTGACTGTTTCGGGCCAGTTGCAAGTCGAAACTTATGCGTGTGCACTCAGTAGTGTATACACCTTTGGCCCGACCTTCCGTGCCGAAAATTCTCACACTTCAAGACATCTTGCAGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGATGATATGAATTGCGCTGAAGCATATGTGAGATTTCTGTGTCAATGGTTACTTGATAACTGCCTAGATGACATGAAATTTATGGCTGATAAGTACGACCGAACTTGTATCGATCGTCTCAAATTGGTCTCTTCAACCCCTTTTGTACGAACTTCGTATACGGAAGCTATTGAAATACTTGAGGAAGCCATAAAGAAGGGTAAGGAGTTTGAGAACAAAGTGGAATGGGGAATTGATTTAGCTTCCGAACATGAAAGATACTTGACTGAGGTAGTATTTAAAAAGCCTGTCATCGTCTATAACTATCCAAAGGGAATCAAAGCCTTTTATATGAGGCTCAATGACGACTCGAAAACTGTAGCTGCAATGGATGTTCTTGTACCTAAGGTTGGGGAGTTGATTGGTGGAAGTCAAAGGGAGGAGCGATTCGAGGTTATTCGCGACAGGATTAAGGAGGTAGGTCTTCCACTTGAACCATATGAATGGTATCTTGATCTGAGACGATACGGAACAGTCAAGCACAGCGGTTTTGGTCTCGGCTTCGAGCGAATGCTTCTTTTTGCCACAGGCATTGACAACATCCGAGACGTTATCCCCTTCCCAAGATATCCAGGACGAGCGGACCTATGA

Protein sequence

MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEARKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL

Homology

[Close]

BLAST of Carg16490 vs. NCBI nr
Match: KAG6588323.1 (Asparagine--tRNA ligase, cytoplasmic 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020885.1 Asparagine--tRNA ligase, cytoplasmic 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 572/572 (100.00%), Postives = 572/572 (100.00%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg16490 vs. NCBI nr
Match: XP_022932543.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata])

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 570/572 (99.65%), Postives = 570/572 (99.65%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAVALNDD STYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg16490 vs. NCBI nr
Match: XP_023531470.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1139.4 bits (2946), Expect = 0.0e+00
Identity = 569/572 (99.48%), Postives = 569/572 (99.48%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAVALNDD STYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAVSNLKASKASKQEIGASV ELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg16490 vs. NCBI nr
Match: XP_022965912.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita maxima])

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 565/572 (98.78%), Postives = 566/572 (98.95%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAV LNDD STYKSLKAEFSDRVPIRTIVCRPDGGSGLAG KVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVTLNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVG VDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGLVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAVSNLKASKASKQEIGASV ELK+AKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVLELKQAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg16490 vs. NCBI nr
Match: XP_022961000.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata] >KAG7023917.1 Asparagine--tRNA ligase, cytoplasmic 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 539/572 (94.23%), Postives = 559/572 (97.73%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPP DQL+AV LNDD  TY SLKAEFSDRVPIR+IV RPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRA+AVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAV+ LKASKASK+EIGASV ELKKAKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDM+FMADKYDRTCIDRLKLVSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+ LPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg16490 vs. ExPASy Swiss-Prot
Match: Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 1.5e-247
Identity = 414/574 (72.13%), Postives = 494/574 (86.06%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MA+   PP  QL+AV+L +D ST +  +A+FS+RV IRTI+ RPDGG+GLAG  VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ ++ D+  +V TGTCV VDGV K+PP  K
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q+IEL    V+ VG VD ++YPLPKTKLTLE LRD +HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IV E+G  V+ LKA+KASK+ I A+V+ELK AKE  A ++ER++L+PG+P+KDG +
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY +DFF RQAFLTVSGQLQVETYACALS+VYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FADL+DDMNCAEAYV+++C WLL+ C  DM+ MA  +D  CIDRLKLV+STPF R +YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           AIE+LEEA+ KGKEF+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHS 540
           D KTVAAMDVLVPKVGELIGGSQREER++VI+ RI+E+GLP+EPYEWYLDLRRYGTVKH 
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

Query: 541 GFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GFGLGFERM+LFATG+DNIRDVIPFPRYPG+ADL
Sbjct: 541 GFGLGFERMILFATGLDNIRDVIPFPRYPGKADL 572

[Close]

BLAST of Carg16490 vs. ExPASy Swiss-Prot
Match: Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 2.0e-239
Identity = 411/576 (71.35%), Postives = 483/576 (83.85%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           M +   PP +QL+A  L +D ST +  KA+FSDRV IR+I+    GG+ LAG KVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSIL---GGGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++++ DL ++V TGTCV VDGV K+PP  K
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q IEL  E V+ VG VDP  YPLPKTKLT EFLRD +HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IVKE+GEAV+ LK +KASK+EI ASV++L  AK ++A +EER +LKPG+P+ DGK+
Sbjct: 241 AARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKI 300

Query: 301 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY  DFF RQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FAD+ DDMNCAEAYV+++C+WL+D C DDM+ M    D  C  RL +V+   F R +YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AIEILEEAIKKGK-EFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           AIE LE+A+ +GK  F+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVK 540
           NDD KTVAAMDVLVPKVGELIGGSQREER++VI+ RI+E+GLP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

Query: 541 HSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           H GFGLGFERM+ FATGIDNIRDVIPFPRYPG+ADL
Sbjct: 541 HCGFGLGFERMIQFATGIDNIRDVIPFPRYPGKADL 571

[Close]

BLAST of Carg16490 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 2.1e-143
Identity = 269/525 (51.24%), Postives = 335/525 (63.81%), Query Frame = 0


Query: 50  LAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIV---PTGTC 109
           L GH + + GWV+T R  ++  F F+E+NDGS   N Q+I    +A   Q++    TG  
Sbjct: 18  LIGHDITLKGWVRTVR--NQKTFTFIEINDGSTLSNFQIIATPDIAGYDQLINQLSTGVS 77

Query: 110 VVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNT 169
           V   G   +  P K Q +E++A A+  +G  DP  Y L K + T EFLR   HLRPRTNT
Sbjct: 78  VSAIGTI-VESPGKEQNLEMQATAITIIGKCDPEVYLLQKKRHTFEFLRSIAHLRPRTNT 137

Query: 170 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 229
           I AV R+RNALA+ATH FF K GFLYIHTPI+T SDCEGAG+MFQVTTL           
Sbjct: 138 IGAVTRVRNALAFATHQFFQKRGFLYIHTPIITGSDCEGAGKMFQVTTL----------- 197

Query: 230 IKNPPPSEAEVDEARKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAK 289
                                                           + N AR  E   
Sbjct: 198 -----------------------------------------------DQNNPARTPE--- 257

Query: 290 LKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 349
                    G+VDY +DFF +  +LTVSGQL  E YACALS VYTFGPTFRAENS+TSRH
Sbjct: 258 ---------GRVDYTQDFFGKPTYLTVSGQLNGEIYACALSDVYTFGPTFRAENSNTSRH 317

Query: 350 LAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLV 409
           LAEFWM+EPE+AFADL D+M+CAE Y++++ +++LDNC +DM+F         I RL+ V
Sbjct: 318 LAEFWMIEPEMAFADLNDNMDCAEDYLKYILKYVLDNCQEDMEFFNKHVATDLISRLEHV 377

Query: 410 SSTPFVRTSYTEAIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 469
            +T F R SYT A+ ILE+A KK   FE  V+WG+DL SEHER+L E  F KPVI+ +YP
Sbjct: 378 INTSFERASYTYAVRILEKADKK---FEYPVKWGLDLQSEHERFLAEEFFGKPVILTDYP 437

Query: 470 KGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWY 529
           K IKAFYMR N+D+KTVAAMDVLVPKVGE+IGGSQREER  V+  ++KE  LP E Y WY
Sbjct: 438 KDIKAFYMRTNEDNKTVAAMDVLVPKVGEIIGGSQREERLSVLESKLKEFNLPAEEYWWY 466

Query: 530 LDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           L+LR++G+V HSGFG GFER++ F TG++NIRDVIPFPR+PG+AD
Sbjct: 498 LELRKFGSVPHSGFGAGFERLVQFTTGMENIRDVIPFPRHPGKAD 466

[Close]

BLAST of Carg16490 vs. ExPASy Swiss-Prot
Match: Q7MVE5 (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=asnS PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 5.2e-139
Identity = 255/525 (48.57%), Postives = 327/525 (62.29%), Query Frame = 0


Query: 49  GLAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQI--VPTGTC 108
           GL G ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + A  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNT 168
           + V G   +      Q +E++A A+   G  DPA YPL K   TLEFLR+  HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
             A+ RIR+ +A A HTFF+  G+ Y H P++T SDCEGAG+MFQVTTL           
Sbjct: 137 FGAIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTL----------- 196

Query: 229 IKNPPPSEAEVDEARKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAK 288
             NP                                             +++ R EE   
Sbjct: 197 --NP---------------------------------------------DSLPRTEE--- 256

Query: 289 LKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 348
                    G+VDY +DFF R   LTVSGQL+ E  A AL  +YTFGPTFRAENS+T RH
Sbjct: 257 ---------GQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRH 316

Query: 349 LAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLV 408
           LAEFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F+++ +D+  IDRLK V
Sbjct: 317 LAEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFV 376

Query: 409 SSTPFVRTSYTEAIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 468
              PFVR +YTE I ILEEA+K G +FE  + WG DLASEHERYL EV FK PVI+ +YP
Sbjct: 377 LEKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYP 436

Query: 469 KGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWY 528
           K IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  ++ +  R  E+G+P +   WY
Sbjct: 437 KEIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWY 468

Query: 529 LDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           LD RRYGT  HSGFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 497 LDSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

[Close]

BLAST of Carg16490 vs. ExPASy Swiss-Prot
Match: B2RK42 (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561) OX=431947 GN=asnS PE=3 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.5e-138
Identity = 254/525 (48.38%), Postives = 326/525 (62.10%), Query Frame = 0


Query: 49  GLAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQI--VPTGTC 108
           GL G ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + A  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNT 168
           + V G   +      Q +E++A A+   G  DPA YPL K   TLEFLR+  HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
              + RIR+ +A A HTFF+  G+ Y H P++T SDCEGAG+MFQVTTL           
Sbjct: 137 FGVIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTL----------- 196

Query: 229 IKNPPPSEAEVDEARKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAK 288
             NP                                             +++ R EE   
Sbjct: 197 --NP---------------------------------------------DSLPRTEE--- 256

Query: 289 LKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 348
                    G+VDY +DFF R   LTVSGQL+ E  A AL  +YTFGPTFRAENS+T RH
Sbjct: 257 ---------GQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRH 316

Query: 349 LAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLV 408
           LAEFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F+++ +D+  IDRLK V
Sbjct: 317 LAEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFV 376

Query: 409 SSTPFVRTSYTEAIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 468
              PFVR +YTE I ILEEA+K G +FE  + WG DLASEHERYL EV FK PVI+ +YP
Sbjct: 377 LEKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYP 436

Query: 469 KGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWY 528
           K IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  ++ +  R  E+G+P +   WY
Sbjct: 437 KEIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWY 468

Query: 529 LDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           LD RRYGT  HSGFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 497 LDSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

[Close]

BLAST of Carg16490 vs. ExPASy TrEMBL
Match: A0A6J1F212 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111439035 PE=3 SV=1)

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 570/572 (99.65%), Postives = 570/572 (99.65%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAVALNDD STYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg16490 vs. ExPASy TrEMBL
Match: A0A6J1HLL2 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111465653 PE=3 SV=1)

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 565/572 (98.78%), Postives = 566/572 (98.95%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAV LNDD STYKSLKAEFSDRVPIRTIVCRPDGGSGLAG KVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVTLNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVG VDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGLVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAVSNLKASKASKQEIGASV ELK+AKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVLELKQAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg16490 vs. ExPASy TrEMBL
Match: A0A6J1HAP9 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461633 PE=3 SV=1)

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 539/572 (94.23%), Postives = 559/572 (97.73%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPP DQL+AV LNDD  TY SLKAEFSDRVPIR+IV RPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRA+AVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAV+ LKASKASK+EIGASV ELKKAKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDM+FMADKYDRTCIDRLKLVSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+ LPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg16490 vs. ExPASy TrEMBL
Match: A0A6J1JKK1 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111485413 PE=3 SV=1)

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 540/572 (94.41%), Postives = 558/572 (97.55%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPP DQL+AV LNDD  TY SLKAEFSDRVPIR+IV RPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRA+AVVHVG VDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAV+ LKASKASK+EIGASV ELKKAKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDM+FMADKYDRTCIDRLKLVSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKEV LPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg16490 vs. ExPASy TrEMBL
Match: A0A5D3CE86 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003180 PE=3 SV=1)

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 536/572 (93.71%), Postives = 557/572 (97.38%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPP DQL+AV LN+D S Y+SLKAEFSDRVPIRTIV RPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPTDQLAAVTLNNDASAYESLKAEFSDRVPIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA+VADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRA+AVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFN HGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R IVKEKGEAV++LKA+KASK+EIGASV ELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RNIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRF+CQWLLDNCLDDMKFMA+KYDRTCIDRLK+VSSTPFVR SYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCLDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           E+LEEA+KKGKEFENKVEWGIDLASEHERYLTEVVF+KPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFRKPVIVYNYPKGIKAFYMRLNDDL 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+GLPLEPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg16490 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 856.7 bits (2212), Expect = 1.1e-248
Identity = 414/574 (72.13%), Postives = 494/574 (86.06%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MA+   PP  QL+AV+L +D ST +  +A+FS+RV IRTI+ RPDGG+GLAG  VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ ++ D+  +V TGTCV VDGV K+PP  K
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q+IEL    V+ VG VD ++YPLPKTKLTLE LRD +HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IV E+G  V+ LKA+KASK+ I A+V+ELK AKE  A ++ER++L+PG+P+KDG +
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY +DFF RQAFLTVSGQLQVETYACALS+VYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FADL+DDMNCAEAYV+++C WLL+ C  DM+ MA  +D  CIDRLKLV+STPF R +YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           AIE+LEEA+ KGKEF+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHS 540
           D KTVAAMDVLVPKVGELIGGSQREER++VI+ RI+E+GLP+EPYEWYLDLRRYGTVKH 
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

Query: 541 GFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GFGLGFERM+LFATG+DNIRDVIPFPRYPG+ADL
Sbjct: 541 GFGLGFERMILFATGLDNIRDVIPFPRYPGKADL 572

[Close]

BLAST of Carg16490 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 829.7 bits (2142), Expect = 1.4e-240
Identity = 411/576 (71.35%), Postives = 483/576 (83.85%), Query Frame = 0


Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           M +   PP +QL+A  L +D ST +  KA+FSDRV IR+I+    GG+ LAG KVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSIL---GGGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++++ DL ++V TGTCV VDGV K+PP  K
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q IEL  E V+ VG VDP  YPLPKTKLT EFLRD +HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IVKE+GEAV+ LK +KASK+EI ASV++L  AK ++A +EER +LKPG+P+ DGK+
Sbjct: 241 AARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKI 300

Query: 301 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY  DFF RQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FAD+ DDMNCAEAYV+++C+WL+D C DDM+ M    D  C  RL +V+   F R +YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AIEILEEAIKKGK-EFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           AIE LE+A+ +GK  F+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVK 540
           NDD KTVAAMDVLVPKVGELIGGSQREER++VI+ RI+E+GLP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

Query: 541 HSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           H GFGLGFERM+ FATGIDNIRDVIPFPRYPG+ADL
Sbjct: 541 HCGFGLGFERMIQFATGIDNIRDVIPFPRYPGKADL 571

[Close]

BLAST of Carg16490 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 488.0 bits (1255), Expect = 1.0e-137
Identity = 266/553 (48.10%), Postives = 343/553 (62.03%), Query Frame = 0


Query: 24  YKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCP 83
           + S   EF  ++ I  +    D G    G  + + GWV+T R   + +  F+E+NDGSC 
Sbjct: 81  FGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNIMGWVRTLR--SQSSVTFIEINDGSCL 140

Query: 84  QNLQVIIEAAVADLGQI----VPTGTCVVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDP 143
            NLQ ++ +      Q+    + TG  V V G        K QK+EL+ E ++ VG  D 
Sbjct: 141 SNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIVASQGTK-QKVELKVEKIIVVGECD- 200

Query: 144 ARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVT 203
           + YP+ K +++ EFLR   HLRPRTNT  AVAR+RN LAYATH FF + GF+++ +PI+T
Sbjct: 201 SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQESGFVWVASPIIT 260

Query: 204 TSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEARKIVKEKGEAVSNLKASKA 263
            SDCEGAGE F VTTL+              P S    D                     
Sbjct: 261 ASDCEGAGEQFCVTTLI--------------PSSHENTD--------------------- 320

Query: 264 SKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQV 323
                  S+  + K                    K G +D+ +DFF + AFLTVSGQL  
Sbjct: 321 ------TSIDAIPKT-------------------KGGLIDWSQDFFGKPAFLTVSGQLNG 380

Query: 324 ETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQW 383
           ETYA ALS VYTFGPTFRAENS+TSRHLAEFWM+EPE+AFADL DDM CA AY++++ ++
Sbjct: 381 ETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVKY 440

Query: 384 LLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAIEILEEAIKKGKEFENKVEW 443
           +LDNC +DM+F     ++  I RL  V+   F++  YT+AIEIL   +K  K+F+  V+W
Sbjct: 441 VLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEIL---LKANKKFDFPVKW 500

Query: 444 GIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIG 503
           G+DL SEHERY+TE  F  +PVI+ +YPK IKAFYMR NDD KTVAAMD+LVP++GELIG
Sbjct: 501 GLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDMLVPRIGELIG 560

Query: 504 GSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIR 563
           GSQREER EV+  R+ E+ L  E Y WYLDLRRYG+V H+GFGLGFER++ F TGIDNIR
Sbjct: 561 GSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIR 566

Query: 564 DVIPFPRYPGRAD 572
           DVIPFPR P  A+
Sbjct: 621 DVIPFPRTPASAE 566

[Close]

BLAST of Carg16490 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 485.0 bits (1247), Expect = 8.6e-137
Identity = 263/633 (41.55%), Postives = 382/633 (60.35%), Query Frame = 0


Query: 18  NDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGWVKTGRKADKDA------ 77
           ++D+S      +++S RV ++T++ R D G+GLAG +V +GGWVK+ R   K++      
Sbjct: 13  DNDLSPKPITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLP 72

Query: 78  ------------------------------------------------------------ 137
                                                                       
Sbjct: 73  VVAAPSPSSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKA 132

Query: 138 -------FAFLELNDGSCPQNLQVIIEAAVADL--GQIVPTGTCVVVDGVFKLP-PPDKH 197
                  F FL ++DGS   +LQV++++A++ +   Q++  GTC+V +GV +LP      
Sbjct: 133 VPPPEYVFYFL-ISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAK 192

Query: 198 QKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYAT 257
             IEL AE ++HVG VDP +YPL K +L L  LRDF H RPRT T+ +V R+ +AL  A+
Sbjct: 193 HVIELEAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLAS 252

Query: 258 HTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAE---VD 317
           HTF   HGF Y+  P++TT+   G GEMF+VTTLL + +  E+   K PP  E +   +D
Sbjct: 253 HTFLQYHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEE---KKPPVQEKDGFSID 312

Query: 318 EARKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKV 377
             + ++KEK   + +LK S ++++ + A+V +LKK  +  +++E + K K G   K  K+
Sbjct: 313 TVKAVIKEKTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKL 372

Query: 378 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 437
           D+ +DFF R  +LT SG+  +E+YA AL  VYTFGP F A+    +RHLAE W VE E+A
Sbjct: 373 DFSKDFFGRDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMA 432

Query: 438 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 497
           FA+L D M+CA+ Y +FLC+++L+N  +DMKF++ + D+T   RL+  +S+  +R SYTE
Sbjct: 433 FAELDDAMDCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTE 492

Query: 498 AIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 557
            I +L++A     +FE K EWG+ L +EH  YLT+ ++K PVIV+ YPK IK FY+RLND
Sbjct: 493 VISLLQKATT--TKFETKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLND 552

Query: 558 DSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHS 572
           D KTVAA D++VPKVG +I GSQ EERFE++  RI E G   E +EWYLDLRR+GTVKHS
Sbjct: 553 DKKTVAAFDLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHS 612

[Close]

BLAST of Carg16490 vs. TAIR 10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 1.2e-26
Identity = 91/291 (31.27%), Postives = 146/291 (50.17%), Query Frame = 0


Query: 286 KLKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACA-LSSVYTFGPTFRAENSHTS 345
           KL  G  E  G   +  ++  + A L  S QL  +   C  L  V+  GP FRAE+S T 
Sbjct: 279 KLMAGSSE-GGSAVFRLEYKGQPACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTH 338

Query: 346 RHLAEFWMVEPEIAF-ADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRL 405
           RHL EF  ++ E+       + M+  +    F+   L + C  +++ +  +Y     + L
Sbjct: 339 RHLCEFVGLDVEMEIRKHYSEIMDLVDELFVFIFTSLNERCKKELQAVGKQYP---FEPL 398

Query: 406 KLVSSTPFVRTSYTEAIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKP---- 465
           K +  T  +R ++ E +++L+EA   G E    V+   DL +E ER L ++V +K     
Sbjct: 399 KFLPKT--LRLTFEEGVQMLKEA---GVE----VDPLGDLNTESERKLGQLVLEKYNTEF 458

Query: 466 VIVYNYPKGIKAFY-MRLNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGL 525
            I++ YPK ++ FY M   D+     + DV + +  E+I G+QR    EV+  R  E G+
Sbjct: 459 YILHRYPKAVRPFYTMTCADNPLYSNSFDVFI-RGEEIISGAQRVHIPEVLEQRAGECGI 518

Query: 526 PLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGR 570
            ++    Y+D  RYG   H GFG+G ER+++    ++NIR    FPR P R
Sbjct: 519 DVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALNNIRKTSLFPRDPQR 555

The following BLAST results are available for this feature:

BLAST of Carg16490 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6588323.1	0.0e+00	100.00	Asparagine--tRNA ligase, cytoplasmic 1, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022932543.1	0.0e+00	99.65	asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata]	[more]
XP_023531470.1	0.0e+00	99.48	asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022965912.1	0.0e+00	98.78	asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita maxima]	[more]
XP_022961000.1	0.0e+00	94.23	asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata] >KAG7023917.1 A...	[more]

BLAST of Carg16490 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SW96	1.5e-247	72.13	Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ...	[more]
Q9SSK1	2.0e-239	71.35	Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ...	[more]
Q6MEC9	2.1e-143	51.24	Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G...	[more]
Q7MVE5	5.2e-139	48.57	Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) ...	[more]
B2RK42	1.5e-138	48.38	Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 207...	[more]

BLAST of Carg16490 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F212	0.0e+00	99.65	Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111439035 PE=3 SV=1	[more]
A0A6J1HLL2	0.0e+00	98.78	Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111465653 PE=3 SV=1	[more]
A0A6J1HAP9	0.0e+00	94.23	Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461633 PE=3 SV=1	[more]
A0A6J1JKK1	0.0e+00	94.41	Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111485413 PE=3 SV=1	[more]
A0A5D3CE86	0.0e+00	93.71	Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of Carg16490 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56680.1	1.1e-248	72.13	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT1G70980.1	1.4e-240	71.35	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT4G17300.1	1.0e-137	48.10	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT3G07420.1	8.6e-137	41.55	asparaginyl-tRNA synthetase 2	[more]
AT4G31180.1	1.2e-26	31.27	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 264..284
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 143..571 e-value: 2.8E-107 score: 360.9
None	No IPR available	GENE3D	1.10.287.10		coord: 230..281 e-value: 2.4E-5 score: 26.1
None	No IPR available	GENE3D	2.40.50.140		coord: 34..138 e-value: 4.5E-9 score: 38.0
None	No IPR available	PANTHER	PTHR22594	ASPARTYL/LYSYL-TRNA SYNTHETASE	coord: 1..572
None	No IPR available	PANTHER	PTHR22594:SF52	BNAC03G13340D PROTEIN	coord: 1..572
None	No IPR available	CDD	cd00776	AsxRS_core	coord: 148..568 e-value: 1.99725E-142 score: 412.73
None	No IPR available	CDD	cd04318	EcAsnRS_like_N	coord: 54..135 e-value: 3.93365E-25 score: 97.2508
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 132..571
IPR002312	Aspartyl/Asparaginyl-tRNA synthetase, class IIb	PRINTS	PR01042	TRNASYNTHASP	coord: 327..340 score: 43.83 coord: 487..503 score: 44.79 coord: 311..323 score: 40.73 coord: 527..541 score: 47.27
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 152..214 e-value: 3.5E-12 score: 46.0
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 303..565 e-value: 3.5E-62 score: 210.3
IPR004522	Asparagine-tRNA ligase	TIGRFAM	TIGR00457	TIGR00457	coord: 51..571 e-value: 1.9E-166 score: 552.3
IPR004522	Asparagine-tRNA ligase	HAMAP	MF_00534	Asn_tRNA_synth	coord: 37..572 score: 41.937798
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 329..562 score: 13.735352
IPR000738	WHEP-TRS domain	PROSITE	PS51185	WHEP_TRS_2	coord: 236..292 score: 9.966467
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 48..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16490-RA	Carg16490-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006421	asparaginyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004816	asparagine-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding