Carg16441 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg16441 vs. NCBI nr
Match: KAG7020837.1 (Protein NRT1/ PTR FAMILY 4.5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 574/574 (100.00%), Postives = 574/574 (100.00%), Query Frame = 0

Query: 1   MEGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60
           MEGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT
Sbjct: 1   MEGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTQALVIVTVQAYSHDLLPKADCPKDCVRGRIAFVFY 120
           AANTLTNFLGSAFLLSLLGGFLSDTQALVIVTVQAYSHDLLPKADCPKDCVRGRIAFVFY
Sbjct: 61  AANTLTNFLGSAFLLSLLGGFLSDTQALVIVTVQAYSHDLLPKADCPKDCVRGRIAFVFY 120

Query: 121 TSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGAAVGVTMIVW 180
           TSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGAAVGVTMIVW
Sbjct: 121 TSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGAAVGVTMIVW 180

Query: 181 VAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRIIQITHTNQLRCLDKA 240
           VAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRIIQITHTNQLRCLDKA
Sbjct: 181 VAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRIIQITHTNQLRCLDKA 240

Query: 241 SILPKDVEPQPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMD 300
           SILPKDVEPQPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMD
Sbjct: 241 SILPKDVEPQPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMD 300

Query: 301 KSLGHLQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA 360
           KSLGHLQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA
Sbjct: 301 KSLGHLQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA 360

Query: 361 VSMAVAGLVEVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGM 420
           VSMAVAGLVEVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGM
Sbjct: 361 VSMAVAGLVEVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGM 420

Query: 421 RSLSTSFTWLSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWF 480
           RSLSTSFTWLSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWF
Sbjct: 421 RSLSTSFTWLSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWF 480

Query: 481 LAILSLLNFFHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCH 540
           LAILSLLNFFHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCH
Sbjct: 481 LAILSLLNFFHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCH 540

Query: 541 HSEEKEDAPIFHEEEERSEIDLKGRQSEEKEVKN 575
           HSEEKEDAPIFHEEEERSEIDLKGRQSEEKEVKN
Sbjct: 541 HSEEKEDAPIFHEEEERSEIDLKGRQSEEKEVKN 574

BLAST of Carg16441 vs. NCBI nr
Match: KAG6586059.1 (Protein NRT1/ PTR FAMILY 4.5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 572/625 (91.52%), Postives = 573/625 (91.68%), Query Frame = 0

Query: 1   MEGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60
           M+GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT
Sbjct: 1   MQGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTQ----------------ALVIVTVQAYSHDLLPKA 120
           AANTLTNFLGSAFLLSLLGGFLSDT                 ALVIVTVQAYSHDLLPKA
Sbjct: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKA 120

Query: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180
           DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL
Sbjct: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180

Query: 181 LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240
           LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR
Sbjct: 181 LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240

Query: 241 IIQ-----------------------------------ITHTNQLRCLDKASILPKDVEP 300
           IIQ                                   ITHTNQLRCLDKASILPKDVEP
Sbjct: 241 IIQVIVVAVKNRGLALPDTPNELYELSDKYYMDSIDSKITHTNQLRCLDKASILPKDVEP 300

Query: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360
           QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP
Sbjct: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360

Query: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420
           APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV
Sbjct: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420

Query: 421 EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW
Sbjct: 421 EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480

Query: 481 LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540
           LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF
Sbjct: 481 LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540

Query: 541 FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKEDAP 575
           FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKEDAP
Sbjct: 541 FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKEDAP 600

BLAST of Carg16441 vs. NCBI nr
Match: XP_022938323.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita moschata])

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 570/625 (91.20%), Postives = 571/625 (91.36%), Query Frame = 0

Query: 1   MEGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60
           M+GSEEKEINVV PTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT
Sbjct: 1   MQGSEEKEINVVGPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTQ----------------ALVIVTVQAYSHDLLPKA 120
           AANTLTNFLGSAFLLSLLGGFLSDT                 ALVIVTVQAYSHDLLPKA
Sbjct: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKA 120

Query: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180
           DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL
Sbjct: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180

Query: 181 LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240
           LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR
Sbjct: 181 LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240

Query: 241 IIQ-----------------------------------ITHTNQLRCLDKASILPKDVEP 300
           IIQ                                   I HTNQLRCLDKASILPKDVEP
Sbjct: 241 IIQVIVVAVKNRGLALPDTPNELYELSDKYYMDSIDSKIAHTNQLRCLDKASILPKDVEP 300

Query: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360
           QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP
Sbjct: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360

Query: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420
           APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV
Sbjct: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420

Query: 421 EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW
Sbjct: 421 EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480

Query: 481 LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540
           LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF
Sbjct: 481 LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540

Query: 541 FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKEDAP 575
           FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKEDAP
Sbjct: 541 FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKEDAP 600

BLAST of Carg16441 vs. NCBI nr
Match: XP_023537955.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 562/625 (89.92%), Postives = 571/625 (91.36%), Query Frame = 0

Query: 1   MEGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60
           M+GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFL+VLHFDLQT
Sbjct: 1   MQGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLLVLHFDLQT 60

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTQ----------------ALVIVTVQAYSHDLLPKA 120
           AANTLTNFLGSAFLLSLLGGFLSDT                 ALVIVTVQAYSHDLLPKA
Sbjct: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKA 120

Query: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180
           DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL
Sbjct: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180

Query: 181 LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240
           LSVVIGAAVGVT+IVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR
Sbjct: 181 LSVVIGAAVGVTLIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240

Query: 241 IIQ-----------------------------------ITHTNQLRCLDKASILPKDVEP 300
           IIQ                                   I HTNQLRCLDKASILPKDVEP
Sbjct: 241 IIQVIVVAIKNRGLALPDTPSELYELSNKYYMDSIDSKIAHTNQLRCLDKASILPKDVEP 300

Query: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360
           QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP
Sbjct: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360

Query: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420
           APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV
Sbjct: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420

Query: 421 EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           EVKRRH+ATKHP+EPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW
Sbjct: 421 EVKRRHRATKHPNEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480

Query: 481 LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540
           LSLSFGYYLSSIFVNVINK+TRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF
Sbjct: 481 LSLSFGYYLSSIFVNVINKVTRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540

Query: 541 FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKEDAP 575
           FHYLYWASWYKYKTEE+IVKLD+GGGGEREREEGVPILREEERSGGDIKCHHSE KEDAP
Sbjct: 541 FHYLYWASWYKYKTEETIVKLDNGGGGEREREEGVPILREEERSGGDIKCHHSEGKEDAP 600

BLAST of Carg16441 vs. NCBI nr
Match: XP_022969591.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita maxima])

HSP 1 Score: 1042.0 bits (2693), Expect = 2.0e-300
Identity = 551/625 (88.16%), Postives = 558/625 (89.28%), Query Frame = 0

Query: 1   MEGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60
           M+GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT
Sbjct: 1   MQGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTQ----------------ALVIVTVQAYSHDLLPKA 120
           AANTLTNFLGSAFLLSLLGGFLSDT                 ALVIVTVQAYSHDLLPKA
Sbjct: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKA 120

Query: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180
           DCPKDCV GRIAFVFYTSL LLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL
Sbjct: 121 DCPKDCVHGRIAFVFYTSLCLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180

Query: 181 LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240
           LSVVIGAAVGVT+IVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR
Sbjct: 181 LSVVIGAAVGVTLIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240

Query: 241 IIQ-----------------------------------ITHTNQLRCLDKASILPKDVEP 300
           IIQ                                   I  T QLR LDKASILPKDVEP
Sbjct: 241 IIQVIVVAIKNRGLALPNTPSELYELSDKYYMDSIESKIARTYQLRFLDKASILPKDVEP 300

Query: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360
           QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNT+IMDKSLGHLQFP
Sbjct: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTKIMDKSLGHLQFP 360

Query: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420
           APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV
Sbjct: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420

Query: 421 EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           E+KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW
Sbjct: 421 EMKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480

Query: 481 LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540
           LSLSFGYYLSSIFVNVINK+TRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF
Sbjct: 481 LSLSFGYYLSSIFVNVINKVTRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540

Query: 541 FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKEDAP 575
           FHYLYWASWYKYKTEE IVKL+DGGGGEREREE VPIL  EERSGG IKCHHSEEKE AP
Sbjct: 541 FHYLYWASWYKYKTEEPIVKLNDGGGGEREREESVPIL-HEERSGGAIKCHHSEEKEHAP 600

BLAST of Carg16441 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.6e-103
Identity = 221/549 (40.26%), Postives = 317/549 (57.74%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAANTLTNFLGSAFLLSLLG 79
           GG  A  F+ A+   E++ F+AN  +LV Y    +H  L  +++ +T F+ +AFLL+LLG
Sbjct: 29  GGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLG 88

Query: 80  GFLSDT----------------QALVIVTVQAYSHDLLP---KADCPKDC--VRGRIAFV 139
           GFL+D                   L+++T+QA    L+P   K+     C  V G  A  
Sbjct: 89  GFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAF 148

Query: 140 FYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGAAVGVTMI 199
            +  LYL+++G GG++G+LP+ GA+QF++  PK  K  +TFFN  +  +  GA V VT +
Sbjct: 149 LFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFV 208

Query: 200 VWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRIIQI---------- 259
           VW+  NK W WGF +S ++  +  +VF LG  FY+  +P  SPL  I ++          
Sbjct: 209 VWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCS 268

Query: 260 ------------------------THTNQLRCLDKASILPKDVEPQPWKVCSVTQVEEVK 319
                                   + TN L CL+KA    +      W  C+V QVE+VK
Sbjct: 269 SKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAI---EGKTHHIWLECTVQQVEDVK 328

Query: 320 IITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAPSIPVIPLVFMAFL 379
           I+ +MLPIF  TI++N CLAQL T+SV Q  T  M++ + +   P+ S+PV P+VFM  L
Sbjct: 329 IVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFPVVFMLIL 388

Query: 380 IPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEVKRRHQATK----- 439
            P Y+ + +PFARK+T    GIT LQR+GVGLVLS V+MAVA LVE+KR+  A +     
Sbjct: 389 APTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLD 448

Query: 440 -HPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLSFGYYL 499
                P++  W++ QY   G AD+FTL GLLEFF+ EAP  MRSL+TS +W SL+ GYYL
Sbjct: 449 SEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYL 508

Query: 500 SSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFHYLYWASW 508
           SS+ V ++N++T+  S  +  W    + E LN N L+LFYW + +LS++NF HYL+WA  
Sbjct: 509 SSVMVPIVNRVTK--SAGQSPW----LGEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKR 566

BLAST of Carg16441 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 9.7e-101
Identity = 219/564 (38.83%), Postives = 315/564 (55.85%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAANTLTNFLGSAFLLSLLG 79
           GG  A  F+  +   E++ ++AN  +LV Y    +H     +AN +TNF+G+AFLL+LLG
Sbjct: 27  GGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLG 86

Query: 80  GFLSDT----------------QALVIVTVQAYSHDLLPKADCPKDC--VRGRIAFVFYT 139
           GFLSD                   L+I+T+QA +  L+P +     C  V G  A + + 
Sbjct: 87  GFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCDSPTCEEVSGSKAAMLFV 146

Query: 140 SLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGAAVGVTMIVWV 199
            LYL+A+G GG++G+L + GA+QF++  PK  K  +TFFN  +  +  GA V VT +VW+
Sbjct: 147 GLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWL 206

Query: 200 AVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRIIQITHTNQLRCLDKA- 259
             NK W WGF +S +A  V  ++F  G  FYR  +P  SPL  I+++     ++C     
Sbjct: 207 EDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGS 266

Query: 260 --------SILP---------KDVEPQ--------------------PWKV--------- 319
                   S+ P         K+VE Q                      KV         
Sbjct: 267 SSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKP 326

Query: 320 ------CSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQF 379
                 C+V QVE+VKI+ +MLPIF  TI++N CLAQL TFSV+Q  +  M+  +G L+ 
Sbjct: 327 VHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAAS--MNTKIGSLKI 386

Query: 380 PAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGL 439
           P  S+P+ P+VF+  L P+Y+ + +PFARK T   +G+T LQR+GVGLVLS ++MAVA L
Sbjct: 387 PPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAAL 446

Query: 440 VEVKRRHQA------TKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRS 499
           VE+KR+  A            P++  W++ QY   G AD+FTL GLLE+F+ EAP  MRS
Sbjct: 447 VEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRS 506

Query: 500 LSTSFTWLSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLA 507
           L+TS +W SL+ GYYLSS+ V+++N IT   S     W+ G   + +N   L+ FYW + 
Sbjct: 507 LATSLSWASLAMGYYLSSVIVSIVNSITG--SSGNTPWLRG---KSINRYKLDYFYWLMC 566

BLAST of Carg16441 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 6.5e-97
Identity = 218/545 (40.00%), Postives = 314/545 (57.61%), Query Frame = 0

Query: 14  PTNEGK-GGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAANTLTNFLGSA 73
           P+N  K GG RA +F+  L  FE MG  A   +L+ Y +  +HF L  AAN +TNF+G+ 
Sbjct: 36  PSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTI 95

Query: 74  FLLSLLGGFLSDT----------------QALVIVTVQAYSHDLLPKADCP---KDC--V 133
           F+ +LLGG+LSD                    ++++VQA+   L P    P   + C   
Sbjct: 96  FIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLIDQTCEEA 155

Query: 134 RGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGA 193
           +G  A +F+ +LYL+A+GSG V+  + A GADQF+Q  PK++K L+++FN    +  +G 
Sbjct: 156 KGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGE 215

Query: 194 AVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQS---PLLRII-- 253
            + +T++VWV  +     GF +SA A  +G I    G  ++R   P +S   P+  +I  
Sbjct: 216 LIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVA 275

Query: 254 ------------------------------QITHTNQLRCLDKASILPKD--VEPQPWKV 313
                                          + HT + R LDKA I  +D   +  PW++
Sbjct: 276 AILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRL 335

Query: 314 CSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGH-LQFPAPSI 373
           C+VTQVE+VK +  ++PIF STI+ NT LAQLQTFSV+QG++  M+  L +    P  S+
Sbjct: 336 CTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSS--MNTRLSNSFHIPPASL 395

Query: 374 PVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEVKR 433
             IP + + FL+PLY+   VPFARK+T H SGI  L R+G+GL LS  SM  A ++E KR
Sbjct: 396 QAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKR 455

Query: 434 RHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLS 493
           R  +       +S+FW++ Q+ IFGI++MFT VGL+EFFYK++  GM S   + T+ S S
Sbjct: 456 RDSSVL-DGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYS 515

Query: 494 FGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFHYL 499
           FG+Y SS+ V+V+NKIT   S   KGW   L   DLN + L+LFYW LA+LSLLNF  YL
Sbjct: 516 FGFYFSSVLVSVVNKIT-STSVDSKGW---LGENDLNKDRLDLFYWLLAVLSLLNFLSYL 573

BLAST of Carg16441 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 2.6e-90
Identity = 211/539 (39.15%), Postives = 302/539 (56.03%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAANTLTNFLGSAFLLSLLG 79
           GG RA +F+     FE M   A   +L+ Y    +HF L  +AN +TNF+G+ FLLSLLG
Sbjct: 40  GGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLG 99

Query: 80  GFLSDT----------------QALVIVTVQAYSHDLLPKADCPKD----CV--RGRIAF 139
           GFLSD+                   ++++VQA+  +L P     K     CV   G  A 
Sbjct: 100 GFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAA 159

Query: 140 VFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGAAVGVTM 199
             YT+L L+A+GSG ++  + + GA+QF +KD    + L++FFN    +  +G  + +T+
Sbjct: 160 TLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTL 219

Query: 200 IVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQS----------------- 259
           +VWV  +     GF +SA   A G I    G  FYR   P  S                 
Sbjct: 220 LVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRK 279

Query: 260 ---------------PLLRIIQITHTNQLRCLDKASILPK-DVEPQPWKVCSVTQVEEVK 319
                           L+R+  + H+N+ R LDKA I  +      PW++C++ QV +VK
Sbjct: 280 QICPSNPNMVHQPSTDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRLCTIEQVHQVK 339

Query: 320 IITRMLPIFISTIIMNTCLAQLQTFSVEQG---NTQIMDKSLGHLQFPAPSIPVIPLVFM 379
           I+  ++PIF  TII NT LAQLQTFSV+QG   NT I        Q P  S+  IP + +
Sbjct: 340 ILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKT----FQIPPASLQAIPYIIL 399

Query: 380 AFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEVKRRHQATKHP 439
            F +PLYE  FVP ARK+T + SGI+ LQR+G GL L+  SM  A LVE KRR ++    
Sbjct: 400 IFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRR-ESFLEQ 459

Query: 440 DEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLSFGYYLSSI 499
           +  +S+FW++ Q+ IFG+++MFT VGL+EFFYK++   M+S  T+ T+ S SFG+YLSS+
Sbjct: 460 NVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSV 519

BLAST of Carg16441 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 333.6 bits (854), Expect = 4.5e-90
Identity = 205/551 (37.21%), Postives = 298/551 (54.08%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAANTLTNFLGSAFLLSLLG 79
           GG  A  F+  +   E++ F+AN  +LV Y    + F    AAN +T F+G+AF L+LLG
Sbjct: 58  GGMLAASFVLVVEVLENLAFLANASNLVLYLSTKMGFSPSGAANAVTAFMGTAFFLALLG 117

Query: 80  GFLSDT----------------QALVIVTVQAYSHDLLPKADCPKDCVRGRIAFVFYTSL 139
           GFL+D                   L+++TVQA+ H   P +               +  L
Sbjct: 118 GFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEHSTEPWS-----------RVFLFVGL 177

Query: 140 YLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGAAVGVTMIVWVAV 199
           YL+A+G GG++G+LP  GA+QF+++     +  + FFN  + S+  GA + VT++VW+  
Sbjct: 178 YLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRSFFFNYFIFSLSCGALIAVTVVVWLED 237

Query: 200 NKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLL-------------------- 259
           NK W +GF +S  A  +   VF  G   YRL VP  SP+                     
Sbjct: 238 NKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVPSGSPITTLFKVLTAALYAKYKKRRTS 297

Query: 260 RIIQITHTNQLRCLDKAS------------------ILPKDVEPQPWKVCSVTQVEEVKI 319
           RI+   HT    C D  +                  +  ++  P+P + C+  QV++VKI
Sbjct: 298 RIVVTCHTRN-DCDDSVTKQNCDGDDGFLGSFLGEVVRERESLPRPLR-CTEEQVKDVKI 357

Query: 320 ITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAPSIPVIPLVFMAFLI 379
           + ++LPIF+STI++N CLAQL TFSV+Q +T  M+  LG    P  ++PV P+VFM  L 
Sbjct: 358 VIKILPIFMSTIMLNCCLAQLSTFSVQQAST--MNTKLGSFTVPPAALPVFPVVFMMILA 417

Query: 380 PLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEVKRRH---------- 439
           P Y  + +P ARK T   +GIT LQR+G GLVLS V+MAVA LVE KR+H          
Sbjct: 418 PTYNHLLLPLARKSTKTETGITHLQRIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNN 477

Query: 440 -QATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLSF 499
             +      P++  W++ QY   G AD+FTL G++EFF+ EAP  MRSL+TS +W SL+ 
Sbjct: 478 SSSYSSSPLPITFLWVAIQYVFLGSADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAM 537

Query: 500 GYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFHYLY 506
           GYY SS+ V+ +N +T         W   L+ E+LN  +L  FYW + +LS +NF HYL+
Sbjct: 538 GYYFSSVLVSAVNFVTG--LNHHNPW---LLGENLNQYHLERFYWLMCVLSGINFLHYLF 588

BLAST of Carg16441 vs. ExPASy TrEMBL
Match: A0A6J1FIK0 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111444459 PE=3 SV=1)

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 570/625 (91.20%), Postives = 571/625 (91.36%), Query Frame = 0

Query: 1   MEGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60
           M+GSEEKEINVV PTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT
Sbjct: 1   MQGSEEKEINVVGPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTQ----------------ALVIVTVQAYSHDLLPKA 120
           AANTLTNFLGSAFLLSLLGGFLSDT                 ALVIVTVQAYSHDLLPKA
Sbjct: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKA 120

Query: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180
           DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL
Sbjct: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180

Query: 181 LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240
           LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR
Sbjct: 181 LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240

Query: 241 IIQ-----------------------------------ITHTNQLRCLDKASILPKDVEP 300
           IIQ                                   I HTNQLRCLDKASILPKDVEP
Sbjct: 241 IIQVIVVAVKNRGLALPDTPNELYELSDKYYMDSIDSKIAHTNQLRCLDKASILPKDVEP 300

Query: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360
           QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP
Sbjct: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360

Query: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420
           APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV
Sbjct: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420

Query: 421 EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW
Sbjct: 421 EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480

Query: 481 LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540
           LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF
Sbjct: 481 LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540

Query: 541 FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKEDAP 575
           FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKEDAP
Sbjct: 541 FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKEDAP 600

BLAST of Carg16441 vs. ExPASy TrEMBL
Match: A0A6J1HY86 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111468570 PE=3 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 9.5e-301
Identity = 551/625 (88.16%), Postives = 558/625 (89.28%), Query Frame = 0

Query: 1   MEGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60
           M+GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT
Sbjct: 1   MQGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTQ----------------ALVIVTVQAYSHDLLPKA 120
           AANTLTNFLGSAFLLSLLGGFLSDT                 ALVIVTVQAYSHDLLPKA
Sbjct: 61  AANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKA 120

Query: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180
           DCPKDCV GRIAFVFYTSL LLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL
Sbjct: 121 DCPKDCVHGRIAFVFYTSLCLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180

Query: 181 LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240
           LSVVIGAAVGVT+IVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR
Sbjct: 181 LSVVIGAAVGVTLIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240

Query: 241 IIQ-----------------------------------ITHTNQLRCLDKASILPKDVEP 300
           IIQ                                   I  T QLR LDKASILPKDVEP
Sbjct: 241 IIQVIVVAIKNRGLALPNTPSELYELSDKYYMDSIESKIARTYQLRFLDKASILPKDVEP 300

Query: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360
           QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNT+IMDKSLGHLQFP
Sbjct: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTKIMDKSLGHLQFP 360

Query: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420
           APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV
Sbjct: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420

Query: 421 EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           E+KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW
Sbjct: 421 EMKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480

Query: 481 LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540
           LSLSFGYYLSSIFVNVINK+TRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF
Sbjct: 481 LSLSFGYYLSSIFVNVINKVTRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540

Query: 541 FHYLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKEDAP 575
           FHYLYWASWYKYKTEE IVKL+DGGGGEREREE VPIL  EERSGG IKCHHSEEKE AP
Sbjct: 541 FHYLYWASWYKYKTEEPIVKLNDGGGGEREREESVPIL-HEERSGGAIKCHHSEEKEHAP 600

BLAST of Carg16441 vs. ExPASy TrEMBL
Match: A0A6J1DQ47 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 4.5-like OS=Momordica charantia OX=3673 GN=LOC111023287 PE=3 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 3.3e-253
Identity = 474/634 (74.76%), Postives = 516/634 (81.39%), Query Frame = 0

Query: 3   GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAA 62
           GSE +EI  VDP+N+GKGGFRAT+FIFALL+FE+MGFVANMVSLVQYFL+V+HFDLQTAA
Sbjct: 71  GSEAREIKDVDPSNKGKGGFRATIFIFALLSFENMGFVANMVSLVQYFLLVMHFDLQTAA 130

Query: 63  NTLTNFLGSAFLLSLLGGFLSDTQ----------------ALVIVTVQAYSHDLLPKADC 122
           NTLTNFLGSAFLLSLLGGFLSDT                 ALV++TVQAYS DLLPK DC
Sbjct: 131 NTLTNFLGSAFLLSLLGGFLSDTYINRLTTSLIFGVLEILALVMITVQAYSQDLLPKPDC 190

Query: 123 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182
            KDCVRGRIAFVFYTSLYLLA+GSGGVRGALPALGA QF+QKDP+EAKAL TFFNC+LLS
Sbjct: 191 LKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGAXQFDQKDPREAKALPTFFNCMLLS 250

Query: 183 VVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242
           VV+GAAVGVT+IVWVAVNKAW+WGF ISA+ATAVGF+VFA+GKPFYRLHVPG+SP+ RII
Sbjct: 251 VVVGAAVGVTVIVWVAVNKAWFWGFLISAVATAVGFVVFAVGKPFYRLHVPGESPVSRII 310

Query: 243 Q-----------------------------------ITHTNQLRCLDKASILPKDVEPQP 302
           Q                                   I HTNQLR LDKA+ILP D E Q 
Sbjct: 311 QVIVVAIRNRRLPLPDSPSELYEISDKYYLDSIDSKIAHTNQLRFLDKAAILPNDSEAQA 370

Query: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAP 362
           WKVCSVTQVEEVKIITRM+PIFISTIIMNTCLAQLQTFSVEQGNT IMD+SLGHLQFPAP
Sbjct: 371 WKVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTTIMDRSLGHLQFPAP 430

Query: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEV 422
           SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA+SMAVAGLVEV
Sbjct: 431 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 490

Query: 423 KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482
           KRR+QAT HP++PMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS
Sbjct: 491 KRRNQATNHPNQPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 550

Query: 483 LSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542
           LSFGYYLSSIFVNV+NK+TR ++PSKKGWVEG IPEDLN+NNLNLFYWFLAILS+LNFFH
Sbjct: 551 LSFGYYLSSIFVNVVNKVTRNIAPSKKGWVEGQIPEDLNHNNLNLFYWFLAILSILNFFH 610

Query: 543 YLYWASWYKYKTEESIVKLDDGGG-------------GEREREEGVPILREEERSGGDIK 572
           YLYWASWYKYKTEE I +LD GGG             G    EEG  I+++    G    
Sbjct: 611 YLYWASWYKYKTEEPIAELDGGGGEDAPIVQDNRASNGGGGGEEGASIIQDNRAEG---- 670

BLAST of Carg16441 vs. ExPASy TrEMBL
Match: A0A1S4E0E0 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo OX=3656 GN=LOC103493841 PE=3 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 1.8e-251
Identity = 469/666 (70.42%), Postives = 511/666 (76.73%), Query Frame = 0

Query: 1   MEGSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQT 60
           + G+EEK+I VVDP +EGKGGFRATMFIF LLTFESMGFVANM SLVQYFL V+HFDL+T
Sbjct: 15  LRGTEEKDIKVVDPKDEGKGGFRATMFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLET 74

Query: 61  AANTLTNFLGSAFLLSLLGGFLSDTQ----------------ALVIVTVQAYSHDLLPKA 120
           AANTLTNF+GSAFLLSLLGGFLSDT                 AL+++TVQAYSHDLLP  
Sbjct: 75  AANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIFGFLEVIALILITVQAYSHDLLPSP 134

Query: 121 DCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLL 180
            CPKDCV+GRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKAL TFFN +L
Sbjct: 135 LCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYML 194

Query: 181 LSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLR 240
           LSVV+GAAVGVT+IVWVAVNKAWYWGFFISALA  VGFI+FA+GKPFYR+ VPGQSP+LR
Sbjct: 195 LSVVLGAAVGVTVIVWVAVNKAWYWGFFISALAALVGFIIFAIGKPFYRIQVPGQSPILR 254

Query: 241 IIQ-----------------------------------ITHTNQLRCLDKASILPKDVEP 300
           +IQ                                   I HTNQLR LDKA+I+PKD+EP
Sbjct: 255 VIQVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIHYKIVHTNQLRFLDKAAIVPKDIEP 314

Query: 301 QPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFP 360
           QPW VCSVTQVEEVKIITRM+PIFISTIIMNTCLAQLQTFSVEQGNT IMDKSLGH QFP
Sbjct: 315 QPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTIIMDKSLGHFQFP 374

Query: 361 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLV 420
           APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA++MAVAGLV
Sbjct: 375 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMAVAGLV 434

Query: 421 EVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTW 480
           EVKRRHQA KHPDEP+SLFWL+FQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFT+
Sbjct: 435 EVKRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTF 494

Query: 481 LSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNF 540
           LSL+ GYYLSSIFVN++NK+TR ++PSK+GWVEGLIPEDLN+NNLNLFYWFLAILS+LNF
Sbjct: 495 LSLALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSVLNF 554

Query: 541 FHYLYWASWYKYKTEESIVKLDDGGG---------------------------------- 561
           FHYLYWASWYKYKTEE I +L+  GG                                  
Sbjct: 555 FHYLYWASWYKYKTEEPIEELNSKGGETTKEPIAELNSKEGETMTKEPIAELNKQGEEIK 614

BLAST of Carg16441 vs. ExPASy TrEMBL
Match: A0A5A7ULM1 (Protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold323G00540 PE=3 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 3.1e-251
Identity = 469/664 (70.63%), Postives = 510/664 (76.81%), Query Frame = 0

Query: 3   GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAA 62
           G+EEK+I VVDP +EGKGGFRATMFIF LLTFESMGFVANM SLVQYFL V+HFDL+TAA
Sbjct: 2   GTEEKDIKVVDPKDEGKGGFRATMFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAA 61

Query: 63  NTLTNFLGSAFLLSLLGGFLSDTQ----------------ALVIVTVQAYSHDLLPKADC 122
           NTLTNF+GSAFLLSLLGGFLSDT                 AL+++TVQAYSHDLLP   C
Sbjct: 62  NTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIFGFLEVIALILITVQAYSHDLLPSPLC 121

Query: 123 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182
           PKDCV+GRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKAL TFFN +LLS
Sbjct: 122 PKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLS 181

Query: 183 VVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242
           VV+GAAVGVT+IVWVAVNKAWYWGFFISALA  VGFI+FA+GKPFYR+ VPGQSP+LR+I
Sbjct: 182 VVLGAAVGVTVIVWVAVNKAWYWGFFISALAALVGFIIFAIGKPFYRIQVPGQSPILRVI 241

Query: 243 Q-----------------------------------ITHTNQLRCLDKASILPKDVEPQP 302
           Q                                   I HTNQLR LDKA+I+PKD+EPQP
Sbjct: 242 QVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQP 301

Query: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAP 362
           W VCSVTQVEEVKIITRM+PIFISTIIMNTCLAQLQTFSVEQGNT IMDKSLGH QFPAP
Sbjct: 302 WNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTIIMDKSLGHFQFPAP 361

Query: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEV 422
           SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA++MAVAGLVEV
Sbjct: 362 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMAVAGLVEV 421

Query: 423 KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482
           KRRHQA KHPDEP+SLFWL+FQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFT+LS
Sbjct: 422 KRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLS 481

Query: 483 LSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542
           L+ GYYLSSIFVN++NK+TR ++PSK+GWVEGLIPEDLN+NNLNLFYWFLAILS+LNFFH
Sbjct: 482 LALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFH 541

Query: 543 YLYWASWYKYKTEESIVKLDDGGG------------------------------------ 561
           YLYWASWYKYKTEE I +L+  GG                                    
Sbjct: 542 YLYWASWYKYKTEEPIEELNSRGGETTKEPIAELNSKEGETMTKEPIAELNKQGEEIKEP 601

BLAST of Carg16441 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 378.3 bits (970), Expect = 1.1e-104
Identity = 221/549 (40.26%), Postives = 317/549 (57.74%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAANTLTNFLGSAFLLSLLG 79
           GG  A  F+ A+   E++ F+AN  +LV Y    +H  L  +++ +T F+ +AFLL+LLG
Sbjct: 25  GGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLG 84

Query: 80  GFLSDT----------------QALVIVTVQAYSHDLLP---KADCPKDC--VRGRIAFV 139
           GFL+D                   L+++T+QA    L+P   K+     C  V G  A  
Sbjct: 85  GFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAF 144

Query: 140 FYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGAAVGVTMI 199
            +  LYL+++G GG++G+LP+ GA+QF++  PK  K  +TFFN  +  +  GA V VT +
Sbjct: 145 LFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFV 204

Query: 200 VWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRIIQI---------- 259
           VW+  NK W WGF +S ++  +  +VF LG  FY+  +P  SPL  I ++          
Sbjct: 205 VWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCS 264

Query: 260 ------------------------THTNQLRCLDKASILPKDVEPQPWKVCSVTQVEEVK 319
                                   + TN L CL+KA    +      W  C+V QVE+VK
Sbjct: 265 SKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAI---EGKTHHIWLECTVQQVEDVK 324

Query: 320 IITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAPSIPVIPLVFMAFL 379
           I+ +MLPIF  TI++N CLAQL T+SV Q  T  M++ + +   P+ S+PV P+VFM  L
Sbjct: 325 IVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFPVVFMLIL 384

Query: 380 IPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEVKRRHQATK----- 439
            P Y+ + +PFARK+T    GIT LQR+GVGLVLS V+MAVA LVE+KR+  A +     
Sbjct: 385 APTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLD 444

Query: 440 -HPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLSFGYYL 499
                P++  W++ QY   G AD+FTL GLLEFF+ EAP  MRSL+TS +W SL+ GYYL
Sbjct: 445 SEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYL 504

Query: 500 SSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFHYLYWASW 508
           SS+ V ++N++T+  S  +  W    + E LN N L+LFYW + +LS++NF HYL+WA  
Sbjct: 505 SSVMVPIVNRVTK--SAGQSPW----LGEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKR 562

BLAST of Carg16441 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 378.3 bits (970), Expect = 1.1e-104
Identity = 221/549 (40.26%), Postives = 317/549 (57.74%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAANTLTNFLGSAFLLSLLG 79
           GG  A  F+ A+   E++ F+AN  +LV Y    +H  L  +++ +T F+ +AFLL+LLG
Sbjct: 29  GGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLG 88

Query: 80  GFLSDT----------------QALVIVTVQAYSHDLLP---KADCPKDC--VRGRIAFV 139
           GFL+D                   L+++T+QA    L+P   K+     C  V G  A  
Sbjct: 89  GFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAF 148

Query: 140 FYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGAAVGVTMI 199
            +  LYL+++G GG++G+LP+ GA+QF++  PK  K  +TFFN  +  +  GA V VT +
Sbjct: 149 LFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFV 208

Query: 200 VWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRIIQI---------- 259
           VW+  NK W WGF +S ++  +  +VF LG  FY+  +P  SPL  I ++          
Sbjct: 209 VWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCS 268

Query: 260 ------------------------THTNQLRCLDKASILPKDVEPQPWKVCSVTQVEEVK 319
                                   + TN L CL+KA    +      W  C+V QVE+VK
Sbjct: 269 SKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAI---EGKTHHIWLECTVQQVEDVK 328

Query: 320 IITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAPSIPVIPLVFMAFL 379
           I+ +MLPIF  TI++N CLAQL T+SV Q  T  M++ + +   P+ S+PV P+VFM  L
Sbjct: 329 IVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFPVVFMLIL 388

Query: 380 IPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEVKRRHQATK----- 439
            P Y+ + +PFARK+T    GIT LQR+GVGLVLS V+MAVA LVE+KR+  A +     
Sbjct: 389 APTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLD 448

Query: 440 -HPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLSFGYYL 499
                P++  W++ QY   G AD+FTL GLLEFF+ EAP  MRSL+TS +W SL+ GYYL
Sbjct: 449 SEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYL 508

Query: 500 SSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFHYLYWASW 508
           SS+ V ++N++T+  S  +  W    + E LN N L+LFYW + +LS++NF HYL+WA  
Sbjct: 509 SSVMVPIVNRVTK--SAGQSPW----LGEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKR 566

BLAST of Carg16441 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 369.0 bits (946), Expect = 6.9e-102
Identity = 219/564 (38.83%), Postives = 315/564 (55.85%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAANTLTNFLGSAFLLSLLG 79
           GG  A  F+  +   E++ ++AN  +LV Y    +H     +AN +TNF+G+AFLL+LLG
Sbjct: 27  GGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLG 86

Query: 80  GFLSDT----------------QALVIVTVQAYSHDLLPKADCPKDC--VRGRIAFVFYT 139
           GFLSD                   L+I+T+QA +  L+P +     C  V G  A + + 
Sbjct: 87  GFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCDSPTCEEVSGSKAAMLFV 146

Query: 140 SLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGAAVGVTMIVWV 199
            LYL+A+G GG++G+L + GA+QF++  PK  K  +TFFN  +  +  GA V VT +VW+
Sbjct: 147 GLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWL 206

Query: 200 AVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRIIQITHTNQLRCLDKA- 259
             NK W WGF +S +A  V  ++F  G  FYR  +P  SPL  I+++     ++C     
Sbjct: 207 EDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGS 266

Query: 260 --------SILP---------KDVEPQ--------------------PWKV--------- 319
                   S+ P         K+VE Q                      KV         
Sbjct: 267 SSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKP 326

Query: 320 ------CSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQF 379
                 C+V QVE+VKI+ +MLPIF  TI++N CLAQL TFSV+Q  +  M+  +G L+ 
Sbjct: 327 VHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAAS--MNTKIGSLKI 386

Query: 380 PAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGL 439
           P  S+P+ P+VF+  L P+Y+ + +PFARK T   +G+T LQR+GVGLVLS ++MAVA L
Sbjct: 387 PPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAAL 446

Query: 440 VEVKRRHQA------TKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRS 499
           VE+KR+  A            P++  W++ QY   G AD+FTL GLLE+F+ EAP  MRS
Sbjct: 447 VEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRS 506

Query: 500 LSTSFTWLSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLA 507
           L+TS +W SL+ GYYLSS+ V+++N IT   S     W+ G   + +N   L+ FYW + 
Sbjct: 507 LATSLSWASLAMGYYLSSVIVSIVNSITG--SSGNTPWLRG---KSINRYKLDYFYWLMC 566

BLAST of Carg16441 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 356.3 bits (913), Expect = 4.6e-98
Identity = 218/545 (40.00%), Postives = 314/545 (57.61%), Query Frame = 0

Query: 14  PTNEGK-GGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAANTLTNFLGSA 73
           P+N  K GG RA +F+  L  FE MG  A   +L+ Y +  +HF L  AAN +TNF+G+ 
Sbjct: 36  PSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTI 95

Query: 74  FLLSLLGGFLSDT----------------QALVIVTVQAYSHDLLPKADCP---KDC--V 133
           F+ +LLGG+LSD                    ++++VQA+   L P    P   + C   
Sbjct: 96  FIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLIDQTCEEA 155

Query: 134 RGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGA 193
           +G  A +F+ +LYL+A+GSG V+  + A GADQF+Q  PK++K L+++FN    +  +G 
Sbjct: 156 KGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGE 215

Query: 194 AVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQS---PLLRII-- 253
            + +T++VWV  +     GF +SA A  +G I    G  ++R   P +S   P+  +I  
Sbjct: 216 LIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVA 275

Query: 254 ------------------------------QITHTNQLRCLDKASILPKD--VEPQPWKV 313
                                          + HT + R LDKA I  +D   +  PW++
Sbjct: 276 AILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRL 335

Query: 314 CSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGH-LQFPAPSI 373
           C+VTQVE+VK +  ++PIF STI+ NT LAQLQTFSV+QG++  M+  L +    P  S+
Sbjct: 336 CTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSS--MNTRLSNSFHIPPASL 395

Query: 374 PVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEVKR 433
             IP + + FL+PLY+   VPFARK+T H SGI  L R+G+GL LS  SM  A ++E KR
Sbjct: 396 QAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKR 455

Query: 434 RHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLS 493
           R  +       +S+FW++ Q+ IFGI++MFT VGL+EFFYK++  GM S   + T+ S S
Sbjct: 456 RDSSVL-DGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYS 515

Query: 494 FGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFHYL 499
           FG+Y SS+ V+V+NKIT   S   KGW   L   DLN + L+LFYW LA+LSLLNF  YL
Sbjct: 516 FGFYFSSVLVSVVNKIT-STSVDSKGW---LGENDLNKDRLDLFYWLLAVLSLLNFLSYL 573

BLAST of Carg16441 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 334.3 bits (856), Expect = 1.9e-91
Identity = 211/539 (39.15%), Postives = 302/539 (56.03%), Query Frame = 0

Query: 20  GGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAANTLTNFLGSAFLLSLLG 79
           GG RA +F+     FE M   A   +L+ Y    +HF L  +AN +TNF+G+ FLLSLLG
Sbjct: 40  GGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLG 99

Query: 80  GFLSDT----------------QALVIVTVQAYSHDLLPKADCPKD----CV--RGRIAF 139
           GFLSD+                   ++++VQA+  +L P     K     CV   G  A 
Sbjct: 100 GFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAA 159

Query: 140 VFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGAAVGVTM 199
             YT+L L+A+GSG ++  + + GA+QF +KD    + L++FFN    +  +G  + +T+
Sbjct: 160 TLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTL 219

Query: 200 IVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQS----------------- 259
           +VWV  +     GF +SA   A G I    G  FYR   P  S                 
Sbjct: 220 LVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRK 279

Query: 260 ---------------PLLRIIQITHTNQLRCLDKASILPK-DVEPQPWKVCSVTQVEEVK 319
                           L+R+  + H+N+ R LDKA I  +      PW++C++ QV +VK
Sbjct: 280 QICPSNPNMVHQPSTDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRLCTIEQVHQVK 339

Query: 320 IITRMLPIFISTIIMNTCLAQLQTFSVEQG---NTQIMDKSLGHLQFPAPSIPVIPLVFM 379
           I+  ++PIF  TII NT LAQLQTFSV+QG   NT I        Q P  S+  IP + +
Sbjct: 340 ILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKT----FQIPPASLQAIPYIIL 399

Query: 380 AFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEVKRRHQATKHP 439
            F +PLYE  FVP ARK+T + SGI+ LQR+G GL L+  SM  A LVE KRR ++    
Sbjct: 400 IFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRR-ESFLEQ 459

Query: 440 DEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLSFGYYLSSI 499
           +  +S+FW++ Q+ IFG+++MFT VGL+EFFYK++   M+S  T+ T+ S SFG+YLSS+
Sbjct: 460 NVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSV 519