Carg16428 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg16428
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionTranslation initiation factor IF-3
LocationCarg_Chr12: 7316034 .. 7320408 (+)
RNA-Seq ExpressionCarg16428
SyntenyCarg16428
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCCTTCCCACAGCTCAAATCTATGGCTGGGCTCCCCGGCAGATTTCCTTTCAAACCTCTCTTATCCCATTCCTCCGCCGCGCCCACCACCTCTGCCGCCGCGCCCTCCTCCTCCGCCACCGCCACTCCCATCCGGACCTCAATTTCCCCTCTCCCCCTCTCCTCCTCCTCTAAGTCCAATGCCTTCAGATTCCGCCTTCACAACTCCAATTCTCTGCATCTCCTCTTCACAACTTCAACTCGTAGTACTGTACTTGCTCAATACGGCGGTGTTTCTCGGTCTTCAGGACCGGCGGATTCTAAGCGCTCTGACGATGGTCTTGGTGTCGATATTTCCGCCATTAGGTGTGTGTGCCTTATGCTTGCTAGATTTACATGTATGATGGACGAGTTTTGTTCATTATCATTATTATTTATTTATGATTATTTGGATTTCTTATTTGTATGGTTGATTTGTTTAGATCGAATACTGTGAGGCTTATCGATGCACAACAGAACATGGTAGGTGGTTTGTTTGAAGCAATTTTCTGTTTGTTTCCTTTTGCGACATTGCAACACATGTAATTTAGGCGTCGTAGTTTGGAATTTTGACCTTCTTGAAATGAACAAATAGGTTGGAATCGTGTCGAAGGCTCAGGCCATTCAAATGGCTGATGACGCTGAACTTGATTTGGTAGGTCTTCTTGTACCGTTTGTTGATAAAGCAATTTTCTATAATTTGTCTTGGAATTCCAAAGAACCTTGCCCCAAACGTTAATGTTCTTCTGAGGACTAATTGCATAGTTGGCCTTAAGTCGAAACTAATTGGCGGGCTTGAGGAATAGTCGAAGTCATACCCAATCGAAAGCACGAAATTTAAAATGGTCCTTTTAGCTTAAGCTTGATGTTCATCTTAAATCTTAATTCATTTTTTCACTTTATGTTTATTTATTGCCTCTTGGGTTTTGGTTTTTATCTTCAATAAACTGTTCTCTGTATAAACATGAGCATAAAATTGAAAGCTTATGATGATAGTAATCGAAAAATTATAGTTATATGTTATAGTATGTCCTCTAAACTATAAGAATCATCGCATGGTGAATGAATTTCATTTCTGGGTTGTGCATTAGGTCAATTTTACTATATAAAATTGTTGTAACGACCCAGACCCACCGCTAACAGATATTGTCCTCTTTGGGCTTTCCCTTTCGGGCTTCCCCTCAAGGCTTTAAAACACGTTTGCTAGGGAAAGGTTTCCACGCCCTTAGAAGGGTGTTTTGTTCTCCTCCCCAACCAATGTGGGATTTCACAATCCACCCCCTTCAGGACCCAGCATCATCGCTGGCACTCGTTCCTTTCTCCGATTGATGTGGAACCCCCACCAAATCCACCCCCCTTTGGGGCCCAGGACACCACCTCGTGTCTACTCCCCTTCGGGGAACAACCTCCTCGCTGGCACATCACCCGGTGCTTGGCTCTGATACCATTTGTAATGGCCAGGCCCACCGCTAGCAGATATTGTCCTCTTTGGGCTTTCCCTTTCGGGTTTCCCTTCAAGGCTTTAAAACACGTCTGCTAGGGAAAGGTTTCCACACCCTTTATTTCGTTCTCTTCCCCAATCAATGTGGGGTATCACAATTATACTTTCCTTCCCATATGGTATAGACCGGCAAAGAAACGTCTATTTGTCGACACAAGACTTGGATGAATGCTGAGTGATGCATTGTTATTAAGATACATGAGAAAAACTTCATTTGACCTGCGAGTTCATAACATAATACTCTTCCAGCAATCAACAATTTATGAATCTCATTTCACTATTTGTGTATTGTTCACCTTTTAACTTGGATAAAGAAGGTCGTGGACTAATATTTTTTTTTTTACTGGAATCTAGATACTTGTGTGTTCTTTGTGTAGGATTTAGCCAATTCTTTCTATTTCATGCAGGTTGTATTATCACCTGATGCAGATCCTCCAGTTGTCAGAATAATGGATTACAGGCATGCCATTTTCTTTCTCTGATCTCCCAATTCCCTTTATTATCGGAAAAGGTTGTATTTGCTCAAGTCCAGTGCCAATCTTATGAACATTAATGAGTTTACCAATACTTATCAAAAACTTTTTGTGGTGATTTATTTTTCAGTTAGTACTTTAATTTTAATTAAATGGTTACGAAAAAGTAATGTATAACAAATGCTAAGTAGATGGAAATGTTGTACATTAGAATGGAGAAGGAATGAAACTATGGGAGAATTAAAAGGCCACATTGTTTTTCGTTGTTAATTAACTTCAAATGAACATATGATGATTGTTATCTTTATACTGGGAGAATTAACTTTAAGTTCTTGTTACTTTGACGTTGAGATTTTTTTTTTTTTTTCGGTCTCGTTTCTCAGTAAATATAGGTATGAACAGCAAAAGAAGAAAAGGGGACAACAGAAGAAAAGTGCTGGTGAATCTCTTTCTTAATATACTCTGCATATTCTCTTTGGTTCACAATCTTTCAGGTTTGAGGAAAGATACTGACAAAGTGTTGTTTTGAATGTTAGCTACTCGCATGGATTTAAAAGAGCTTAAAATGGGGTAACACATTCCTTCATTGCCTTTTCTTTTTGGAGGTCATCTTTCTTTTCTTTTTCCTTCTGTCTAAAGCAACTCGAACTTTTGACTTCTAAGATCGATAAATAACCTACAAAGAAAAGAGCTATGAGCGGCTTTTGTTCGCTATATCGAAAAAAATTCTGCGAGTATCGTCTTCCCAGTCTGAACCTTCTGGTTGCTATTATTATTGCACAGTTACAACATCGACCAACATGATTACTCTGTCCGTTTGAAAGCTGCAAGGAAGTTTTTGAAAGATGGAGATAAGGTTTCTTTTCTAATCTACCATATCTTTGATTGTCTGTGTTAGGAATCACGACTCTCCACAATGGTATGATATTGTCCACTTTGAGCATAAACTCTCATGGCTTTGCTTTGGGCTTCCCTAAAAGGTCTCATACCAATGGAGATAGTATTCCTCACTTATAAACCCATGATGTTCCACTAAATTAACCAATGTGGGACTCACTCTCAGTAATCCTCAACAATCCTCCCCTCGAACAAAGTACACTATAAGCCTCCCCTGAGGCTTATGGAGCTCTCGAATAGCCTCCTCTTAAATCGAGGCTCGATTCGTTTCTTTGGAGCCCTCGAACAAAGTACACCCTTTGTTCGACACTTTAGTCACTTTTGATTATACCTTCGAGGCTCATAGTTCTTTGATCAACATTTGAGGATTCTATTGACATGGTTAACTTTAGGGCATGACTCTGATACCATGTGTTAGGAATCACGACTCTCCACAATTGTATGATATTGTCCACTTTGAGCATAAGCTCTCATGGCTTTGCTTTGGGCTTCCCCAAAAGGCCTCATACCAATGGAGATAGTATTCCTCACTTATAAACCCATGATCTTCCACTAAATTAACCAACGTGGGACTCACTCTCAATAATCCTCAACAGTCTGTTCATGCATTCAAGTAGATTGTTTCAGTTCGTCGAATATTGTTTCAGTTTGTCTGAATCACTTTGAAATCTTTTAACAAAATGTTATAGGTTAAAGTTATAGTGAACTTGAAAGGTCGTGAGAATGCGTTCAAGAACATAGCAATTGAGCTCATCAGACGTTTTCAGGAAGAGATTGGGGAGGTATTCATTTTCTGTCAATTTTGTGCCACCTCTGTCAATTGTCCATCACTGTGGTATTGTTACTCAATAAGATGCTCCAAATTATTTTAAGTGAAATTTTAAAGGGTAAATTTAGGCACGTGTTTGAACGATATTAAGTCTGATATCATCACCTCATGGAGCACGTTTCGTAGCTCCGGGTAGACTGTTCTTGCAAACAGCTTTTGAAAACTGTTTAATATAACCGTTCTCCGGCTGTGAGATGTCAGCTTAAGCATCCTAGTCTCACGAGGCACTCTACCTAACTCTGTAATATTTTGGTTGATAGTATATGTCTTAACCAATTGAGTTACTCACGTTAGCCCTCAACAATTTATAATATGTAATATTTTAATATCTTAGATAGGTGGCTATAATATATATATATATTTCATTTCAAGTTCTACTAATCTATCTCATTTATATTCCTAATGTGAATTTGATGCCATATTTGTGCTTCTTGTTAATACTTCAAACAAACACAATATATCTTGTGTTTATGTCTGATCCAATGACCTGCTCAATATTTTTATTATACACAGCTTGCAACTGAAGAGGCCAAGAACTTAAGAGACAGGAATATGTTCATTGTTCTAGCTCCTAACAAGGCTGTTCTACAGAAGGCTCAAGAACCCCCCAAGGTAAAAGACAACCTTGTAGTTAATGAAGTTTCTGCCAGTGTTTAA

mRNA sequence

TCCCTTCCCACAGCTCAAATCTATGGCTGGGCTCCCCGGCAGATTTCCTTTCAAACCTCTCTTATCCCATTCCTCCGCCGCGCCCACCACCTCTGCCGCCGCGCCCTCCTCCTCCGCCACCGCCACTCCCATCCGGACCTCAATTTCCCCTCTCCCCCTCTCCTCCTCCTCTAAGTCCAATGCCTTCAGATTCCGCCTTCACAACTCCAATTCTCTGCATCTCCTCTTCACAACTTCAACTCGTAGTACTGTACTTGCTCAATACGGCGGTGTTTCTCGGTCTTCAGGACCGGCGGATTCTAAGCGCTCTGACGATGGTCTTGGTGTCGATATTTCCGCCATTAGATCGAATACTGTGAGGCTTATCGATGCACAACAGAACATGGTTGGAATCGTGTCGAAGGCTCAGGCCATTCAAATGGCTGATGACGCTGAACTTGATTTGGTTGTATTATCACCTGATGCAGATCCTCCAGTTCTTGCAACTGAAGAGGCCAAGAACTTAAGAGACAGGAATATGTTCATTGTTCTAGCTCCTAACAAGGCTGTTCTACAGAAGGCTCAAGAACCCCCCAAGGTAAAAGACAACCTTGTAGTTAATGAAGTTTCTGCCAGTGTTTAA

Coding sequence (CDS)

ATGGCTGGGCTCCCCGGCAGATTTCCTTTCAAACCTCTCTTATCCCATTCCTCCGCCGCGCCCACCACCTCTGCCGCCGCGCCCTCCTCCTCCGCCACCGCCACTCCCATCCGGACCTCAATTTCCCCTCTCCCCCTCTCCTCCTCCTCTAAGTCCAATGCCTTCAGATTCCGCCTTCACAACTCCAATTCTCTGCATCTCCTCTTCACAACTTCAACTCGTAGTACTGTACTTGCTCAATACGGCGGTGTTTCTCGGTCTTCAGGACCGGCGGATTCTAAGCGCTCTGACGATGGTCTTGGTGTCGATATTTCCGCCATTAGATCGAATACTGTGAGGCTTATCGATGCACAACAGAACATGGTTGGAATCGTGTCGAAGGCTCAGGCCATTCAAATGGCTGATGACGCTGAACTTGATTTGGTTGTATTATCACCTGATGCAGATCCTCCAGTTCTTGCAACTGAAGAGGCCAAGAACTTAAGAGACAGGAATATGTTCATTGTTCTAGCTCCTAACAAGGCTGTTCTACAGAAGGCTCAAGAACCCCCCAAGGTAAAAGACAACCTTGTAGTTAATGAAGTTTCTGCCAGTGTTTAA

Protein sequence

MAGLPGRFPFKPLLSHSSAAPTTSAAAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLHNSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQNMVGIVSKAQAIQMADDAELDLVVLSPDADPPVLATEEAKNLRDRNMFIVLAPNKAVLQKAQEPPKVKDNLVVNEVSASV
Homology
[Close]
BLAST of Carg16428 vs. NCBI nr
Match: KAG7020824.1 (Translation initiation factor IF3-2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 363.2 bits (931), Expect = 1.4e-96
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0 

Query: 1   MAGLPGRFPFKPLLSHSSAAPTTSAAAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH 60
           MAGLPGRFPFKPLLSHSSAAPTTSAAAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH
Sbjct: 1   MAGLPGRFPFKPLLSHSSAAPTTSAAAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH 60

Query: 61  NSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQN 120
           NSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQN
Sbjct: 61  NSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQN 120

Query: 121 MVGIVSKAQAIQMADDAELDLVVLSPDADPPVLATEEAKNLRDRNMFIVLAPNKAVLQKA 180
           MVGIVSKAQAIQMADDAELDLVVLSPDADPPVLATEEAKNLRDRNMFIVLAPNKAVLQKA
Sbjct: 121 MVGIVSKAQAIQMADDAELDLVVLSPDADPPVLATEEAKNLRDRNMFIVLAPNKAVLQKA 180

Query: 181 QEPPKVKDNLVVNEVSASV 200
           QEPPKVKDNLVVNEVSASV
Sbjct: 181 QEPPKVKDNLVVNEVSASV 199

[Close]
BLAST of Carg16428 vs. NCBI nr
Match: XP_022937979.1 (translation initiation factor IF3-4, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 308.9 bits (790), Expect = 3.1e-80
Identity = 192/291 (65.98%), Postives = 194/291 (66.67%), Query Frame = 0 

Query: 1   MAGLPGRFPFKPLLSHSSAAPTTSAAAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH 60
           MAGL  RFPFKPLLSHSSA+PTTSAAAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH
Sbjct: 1   MAGLTARFPFKPLLSHSSASPTTSAAAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH 60

Query: 61  NSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQN 120
           NSNSLHLLFTTSTRSTV+AQYGG SR SGPADSKR DDGLGVDISAIRSNTVRLIDAQQN
Sbjct: 61  NSNSLHLLFTTSTRSTVVAQYGGGSRPSGPADSKRFDDGLGVDISAIRSNTVRLIDAQQN 120

Query: 121 MVGIVSKAQAIQMADDAELDLVVLSPDADPPV---------------------------- 180
           MVGIVSKAQAIQMADDAELDLVVLSPDADPPV                            
Sbjct: 121 MVGIVSKAQAIQMADDAELDLVVLSPDADPPVVRIMDYSKYRYEQQKKKRGQQKKSAATR 180

Query: 181 ------------------------------------------------------------ 200
                                                                       
Sbjct: 181 MDLKELKMGYNIDQHDYSVRLKAARKFLKDGDKVKVIVNLKGRENAFKNIAIELIRRFQE 240

[Close]
BLAST of Carg16428 vs. NCBI nr
Match: KAG6586047.1 (Translation initiation factor IF3-2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 305.8 bits (782), Expect = 2.7e-79
Identity = 192/291 (65.98%), Postives = 192/291 (65.98%), Query Frame = 0 

Query: 1   MAGLPGRFPFKPLLSHSSAAPTTSAAAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH 60
           MAGLPGRFPFKPLLSHSS       AAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH
Sbjct: 1   MAGLPGRFPFKPLLSHSS-------AAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH 60

Query: 61  NSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQN 120
           NSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQN
Sbjct: 61  NSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQN 120

Query: 121 MVGIVSKAQAIQMADDAELDLVVLSPDADPPV---------------------------- 180
           MVGIVSKAQAIQMADDAELDLVVLSPDADPPV                            
Sbjct: 121 MVGIVSKAQAIQMADDAELDLVVLSPDADPPVVRIMDYSKYRYEQQKKKRGQQKKSAATR 180

Query: 181 ------------------------------------------------------------ 200
                                                                       
Sbjct: 181 MDLKELKMGYNIDQHDYSVRLKAARKFLKDGDKVKVIVNLKGRENAFKNIAIELIRRFQE 240

[Close]
BLAST of Carg16428 vs. NCBI nr
Match: XP_023536766.1 (translation initiation factor IF3-4, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.0 bits (772), Expect = 3.8e-78
Identity = 189/291 (64.95%), Postives = 193/291 (66.32%), Query Frame = 0 

Query: 1   MAGLPGRFPFKPLLSHSSAAPTTSAAAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH 60
           MAGL GRFPFKPLLSHSSA PTTSA+APSSSATATPIRTS+SPLPL SSSKSNAFRFRLH
Sbjct: 1   MAGLTGRFPFKPLLSHSSATPTTSASAPSSSATATPIRTSLSPLPL-SSSKSNAFRFRLH 60

Query: 61  NSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQN 120
           NSNSLHL+FTTSTRSTV+AQYGG SR SGPADSKR DDGLGVDISAIRSNTVRLIDAQQN
Sbjct: 61  NSNSLHLVFTTSTRSTVVAQYGGGSRPSGPADSKRFDDGLGVDISAIRSNTVRLIDAQQN 120

Query: 121 MVGIVSKAQAIQMADDAELDLVVLSPDADPPV---------------------------- 180
           MVGIVSKAQAIQMADDAELDLVVLSPDADPPV                            
Sbjct: 121 MVGIVSKAQAIQMADDAELDLVVLSPDADPPVVRIMDYSKYRYEQQKKKRGQQKKSAATR 180

Query: 181 ------------------------------------------------------------ 200
                                                                       
Sbjct: 181 MDLKELKMGYNIDQHDYSVRLKAARKFLKDGDKVKVIVNLKGRENAFKNIAIELIRRFQE 240

[Close]
BLAST of Carg16428 vs. NCBI nr
Match: XP_022969585.1 (translation initiation factor IF3-4, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 294.3 bits (752), Expect = 8.0e-76
Identity = 188/293 (64.16%), Postives = 191/293 (65.19%), Query Frame = 0 

Query: 1   MAGLPGRFPFKPLLSHSSAAPTTSAAAPSSSAT--ATPIRTSISPLPLSSSSKSNAFRFR 60
           MAGL GRFPFKPLLSHSSA PTTSAAAPSSSAT  ATPIRTSISPLPL SSSK NAFRFR
Sbjct: 1   MAGLTGRFPFKPLLSHSSATPTTSAAAPSSSATAIATPIRTSISPLPL-SSSKFNAFRFR 60

Query: 61  LHNSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQ 120
            HNSNSLHL+FTTSTRSTV++QYGG SRSSGPADSKR DDGLGVDISAIRSNTVRLIDAQ
Sbjct: 61  HHNSNSLHLVFTTSTRSTVVSQYGGASRSSGPADSKRFDDGLGVDISAIRSNTVRLIDAQ 120

Query: 121 QNMVGIVSKAQAIQMADDAELDLVVLSPDADPPV-------------------------- 180
           QNMVGIVSK QAIQMADDAELDLVVLSPDADPPV                          
Sbjct: 121 QNMVGIVSKVQAIQMADDAELDLVVLSPDADPPVVRIMDYSKYRYEQQKKKRGQQKKSAA 180

Query: 181 ------------------------------------------------------------ 200
                                                                       
Sbjct: 181 TRMDLKELKMGYNIDQHDYSVRLKAARKFLKDGDKVKVIVNLKGRENAFKNIAIELIRRF 240

[Close]
BLAST of Carg16428 vs. ExPASy Swiss-Prot
Match: Q94B52 (Translation initiation factor IF3-4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IF3-4 PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.8e-15
Identity = 47/74 (63.51%), Postives = 57/74 (77.03%), Query Frame = 0 

Query: 82  GGVSRSSGP--ADSKRSDDGLGVDISAIRSNTVRLIDAQQNMVGIVSKAQAIQMADDAEL 141
           GG SR  G      K S+D   +DISAIRS TVRLID QQNM+G+VSK +A++ A+DAEL
Sbjct: 64  GGGSRFPGDRRGRQKESEDDDSLDISAIRSATVRLIDDQQNMIGLVSKEEAVRRAEDAEL 123

Query: 142 DLVVLSPDADPPVL 154
           DLV+LSPDADPPV+
Sbjct: 124 DLVILSPDADPPVV 137


HSP 2 Score: 54.3 bits (129), Expect = 1.8e-06
Identity = 28/47 (59.57%), Postives = 35/47 (74.47%), Query Frame = 0 

Query: 153 LATEEAKNLRDRNMFIVLAPNKAVLQKAQE-PPKVKDNLVVNEVSAS 199
           L TEE+KN RDRN+FIVL PNK V++K QE PPK K     ++VSA+
Sbjct: 227 LGTEESKNFRDRNLFIVLVPNKEVIRKVQEPPPKKKKKPADDKVSAA 273

[Close]
BLAST of Carg16428 vs. ExPASy Swiss-Prot
Match: O82234 (Translation initiation factor IF3-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IF3-2 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 43/74 (58.11%), Postives = 59/74 (79.73%), Query Frame = 0 

Query: 80  QYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQNMVGIVSKAQAIQMADDAEL 139
           ++ G +R   P +++  +    +DIS+IRS TVRLID QQNM+G+VSK +A++MADDAEL
Sbjct: 71  RFSGDNRRGRPKEAEIDE---ALDISSIRSATVRLIDGQQNMLGLVSKDEAVRMADDAEL 130

Query: 140 DLVVLSPDADPPVL 154
           DLV+LSPDADPPV+
Sbjct: 131 DLVILSPDADPPVV 141


HSP 2 Score: 58.2 bits (139), Expect = 1.3e-07
Identity = 29/47 (61.70%), Postives = 35/47 (74.47%), Query Frame = 0 

Query: 153 LATEEAKNLRDRNMFIVLAPNKAVLQKAQEPP-KVKDNLVVNEVSAS 199
           LATEE+KN RDRNMFI+L PNK +++K QEPP + K     NE SAS
Sbjct: 231 LATEESKNFRDRNMFIILVPNKEMIRKPQEPPTRKKKKTAENEASAS 277

[Close]
BLAST of Carg16428 vs. ExPASy Swiss-Prot
Match: Q9CN42 (Translation initiation factor IF-3 OS=Pasteurella multocida (strain Pm70) OX=272843 GN=infC PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.0e-05
Identity = 27/46 (58.70%), Postives = 33/46 (71.74%), Query Frame = 0 

Query: 107 IRSNTVRLIDAQQNMVGIVSKAQAIQMADDAELDLVVLSPDADPPV 153
           IR   VRLID      GIVS  QA++MA+ AELDLV +SP+A+PPV
Sbjct: 20  IRVKEVRLIDQDGEQAGIVSIQQALEMAEQAELDLVEISPNAEPPV 65

[Close]
BLAST of Carg16428 vs. ExPASy Swiss-Prot
Match: Q74IC4 (Translation initiation factor IF-3 OS=Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) OX=257314 GN=infC PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.5e-05
Identity = 26/46 (56.52%), Postives = 34/46 (73.91%), Query Frame = 0 

Query: 107 IRSNTVRLIDAQQNMVGIVSKAQAIQMADDAELDLVVLSPDADPPV 153
           IR+  VRLI      VG+VSK +A++ A DA+LDLV+LSP+A PPV
Sbjct: 7   IRAREVRLIGVDGQQVGVVSKNEALRKAADADLDLVLLSPNAKPPV 52

[Close]
BLAST of Carg16428 vs. ExPASy Swiss-Prot
Match: Q8XJ67 (Translation initiation factor IF-3 OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=infC PE=3 SV=2)

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-04
Identity = 25/46 (54.35%), Postives = 33/46 (71.74%), Query Frame = 0 

Query: 107 IRSNTVRLIDAQQNMVGIVSKAQAIQMADDAELDLVVLSPDADPPV 153
           IR   VRLI +    +G+VS   A +MAD+AELDLV++SP+A PPV
Sbjct: 18  IREKEVRLISSTGEQLGVVSGRDAQRMADEAELDLVMISPNAKPPV 63

[Close]
BLAST of Carg16428 vs. ExPASy TrEMBL
Match: A0A6J1FCR3 (Translation initiation factor IF-3 OS=Cucurbita moschata OX=3662 GN=LOC111444202 PE=3 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 1.5e-80
Identity = 192/291 (65.98%), Postives = 194/291 (66.67%), Query Frame = 0 

Query: 1   MAGLPGRFPFKPLLSHSSAAPTTSAAAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH 60
           MAGL  RFPFKPLLSHSSA+PTTSAAAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH
Sbjct: 1   MAGLTARFPFKPLLSHSSASPTTSAAAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH 60

Query: 61  NSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQN 120
           NSNSLHLLFTTSTRSTV+AQYGG SR SGPADSKR DDGLGVDISAIRSNTVRLIDAQQN
Sbjct: 61  NSNSLHLLFTTSTRSTVVAQYGGGSRPSGPADSKRFDDGLGVDISAIRSNTVRLIDAQQN 120

Query: 121 MVGIVSKAQAIQMADDAELDLVVLSPDADPPV---------------------------- 180
           MVGIVSKAQAIQMADDAELDLVVLSPDADPPV                            
Sbjct: 121 MVGIVSKAQAIQMADDAELDLVVLSPDADPPVVRIMDYSKYRYEQQKKKRGQQKKSAATR 180

Query: 181 ------------------------------------------------------------ 200
                                                                       
Sbjct: 181 MDLKELKMGYNIDQHDYSVRLKAARKFLKDGDKVKVIVNLKGRENAFKNIAIELIRRFQE 240

[Close]
BLAST of Carg16428 vs. ExPASy TrEMBL
Match: A0A6J1I0C0 (Translation initiation factor IF-3 OS=Cucurbita maxima OX=3661 GN=LOC111468564 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 3.9e-76
Identity = 188/293 (64.16%), Postives = 191/293 (65.19%), Query Frame = 0 

Query: 1   MAGLPGRFPFKPLLSHSSAAPTTSAAAPSSSAT--ATPIRTSISPLPLSSSSKSNAFRFR 60
           MAGL GRFPFKPLLSHSSA PTTSAAAPSSSAT  ATPIRTSISPLPL SSSK NAFRFR
Sbjct: 1   MAGLTGRFPFKPLLSHSSATPTTSAAAPSSSATAIATPIRTSISPLPL-SSSKFNAFRFR 60

Query: 61  LHNSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQ 120
            HNSNSLHL+FTTSTRSTV++QYGG SRSSGPADSKR DDGLGVDISAIRSNTVRLIDAQ
Sbjct: 61  HHNSNSLHLVFTTSTRSTVVSQYGGASRSSGPADSKRFDDGLGVDISAIRSNTVRLIDAQ 120

Query: 121 QNMVGIVSKAQAIQMADDAELDLVVLSPDADPPV-------------------------- 180
           QNMVGIVSK QAIQMADDAELDLVVLSPDADPPV                          
Sbjct: 121 QNMVGIVSKVQAIQMADDAELDLVVLSPDADPPVVRIMDYSKYRYEQQKKKRGQQKKSAA 180

Query: 181 ------------------------------------------------------------ 200
                                                                       
Sbjct: 181 TRMDLKELKMGYNIDQHDYSVRLKAARKFLKDGDKVKVIVNLKGRENAFKNIAIELIRRF 240

[Close]
BLAST of Carg16428 vs. ExPASy TrEMBL
Match: A0A6J1DYM5 (Translation initiation factor IF-3 OS=Momordica charantia OX=3673 GN=LOC111024717 PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 4.8e-58
Identity = 155/292 (53.08%), Postives = 171/292 (58.56%), Query Frame = 0 

Query: 1   MAGLPGRFPFKPLLSHSSAAPTTSAAAPSSSATAT-PIRTSISPLPLSSSSKSNAFRFRL 60
           MAGL  RFPFKPLLS SSA  +++AA+ SSSA A  P RTSISPLPL SSS+SNAF FRL
Sbjct: 1   MAGLTSRFPFKPLLSQSSATTSSAAASSSSSAAAAPPFRTSISPLPL-SSSESNAFGFRL 60

Query: 61  HNSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQ 120
           HNSNSL L+ ++  R +V A++GG  RSSGPADSKRSDD  G+D+SAIRSN VRLIDAQQ
Sbjct: 61  HNSNSLRLIVSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLIDAQQ 120

Query: 121 NMVGIVSKAQAIQMADDAELDLVVLSPDADPPV--------------------------- 180
           NMVGIVSKAQAIQMA+DAELDLVVLSPDADPPV                           
Sbjct: 121 NMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAAT 180

Query: 181 ------------------------------------------------------------ 200
                                                                       
Sbjct: 181 RMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQ 240

[Close]
BLAST of Carg16428 vs. ExPASy TrEMBL
Match: A0A1S3BV82 (Translation initiation factor IF-3 OS=Cucumis melo OX=3656 GN=LOC103493630 PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 1.2e-53
Identity = 149/297 (50.17%), Postives = 167/297 (56.23%), Query Frame = 0 

Query: 1   MAGLPGRFPFKPLLSHSSAAPTTS--AAAPSSSATATPIRTSISPLPLSSSSKSNAFRFR 60
           MAGL G FPFK LLS SSAA TT+  AAAP+SS++ TP RTS   LP+SSS  SNAF+ R
Sbjct: 1   MAGLSGSFPFKSLLSQSSAASTTTSPAAAPTSSSSTTPFRTSTPSLPISSSFNSNAFKLR 60

Query: 61  LHNSNSLHLLF----TTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRL 120
           LH SNSL L+F    ++  R++V A+ GG  RSSGP DSKRS+D  G+D+SAIRSN VRL
Sbjct: 61  LHKSNSLRLVFSFPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEFGLDVSAIRSNNVRL 120

Query: 121 IDAQQNMVGIVSKAQAIQMADDAELDLVVLSPDADPPV---------------------- 180
           IDAQQNMVG+VSKAQAIQMA+DAELDLVVLSPDADPPV                      
Sbjct: 121 IDAQQNMVGVVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQK 180

Query: 181 ------------------------------------------------------------ 200
                                                                       
Sbjct: 181 KSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAIEL 240

[Close]
BLAST of Carg16428 vs. ExPASy TrEMBL
Match: A0A6J1G3Y5 (Translation initiation factor IF-3 OS=Cucurbita moschata OX=3662 GN=LOC111450575 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 4.3e-51
Identity = 147/292 (50.34%), Postives = 165/292 (56.51%), Query Frame = 0 

Query: 1   MAGLPGRFPFKPLLSHSSAAPTTSAAAPSSSATATPIRTSISPLPLSSSSKSNAFRFRLH 60
           MAGL GRFPFK + S SSA   T+    SSS++ATP RTSI PLPL  SSKSNAFRFRLH
Sbjct: 1   MAGLSGRFPFKSISSQSSAT-ATATTTTSSSSSATPFRTSIFPLPL-PSSKSNAFRFRLH 60

Query: 61  NSNSLHLLFTTSTRSTVLAQYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQN 120
           NSNSL L+ ++  R++V A+YGG  RSSGPA SKRS+D L +D+S+IRSN VRLID QQN
Sbjct: 61  NSNSLRLV-SSPIRTSVFARYGGAPRSSGPAGSKRSEDSLALDVSSIRSNNVRLIDEQQN 120

Query: 121 MVGIVSKAQAIQMADDAELDLVVLSPDADPPV---------------------------- 180
           MVGIV K+QAIQMA+DAELDLVVLSPDADPPV                            
Sbjct: 121 MVGIVPKSQAIQMAEDAELDLVVLSPDADPPVVRIMDYSKYRYDLQKKKREQQKKSAATR 180

Query: 181 ------------------------------------------------------------ 200
                                                                       
Sbjct: 181 MDLKELKMGYKIDQHDYSVRLRAAKKFLQDGDKVKVIVNLKGRENEFRKIAIELVKRFQE 240

[Close]
BLAST of Carg16428 vs. TAIR 10
Match: AT4G30690.2 (Translation initiation factor 3 protein )

HSP 1 Score: 104.4 bits (259), Expect = 1.1e-22
Identity = 75/182 (41.21%), Postives = 91/182 (50.00%), Query Frame = 0 

Query: 82  GGVSRSSGP--ADSKRSDDGLGVDISAIRSNTVRLIDAQQNMVGIVSKAQAIQMADDAEL 141
           GG SR  G      K S+D   +DISAIRS TVRLID QQNM+G+VSK +A++ A+DAEL
Sbjct: 64  GGGSRFPGDRRGRQKESEDDDSLDISAIRSATVRLIDDQQNMIGLVSKEEAVRRAEDAEL 123

Query: 142 DLVVLSPDADPPV----------------------------------------------- 199
           DLV+LSPDADPPV                                               
Sbjct: 124 DLVILSPDADPPVVRMMDYSYNIDQHDYSVRMRAARKFLQDGDKVKVIVNMKGRENEFRN 183

[Close]
BLAST of Carg16428 vs. TAIR 10
Match: AT4G30690.1 (Translation initiation factor 3 protein )

HSP 1 Score: 83.6 bits (205), Expect = 2.0e-16
Identity = 47/74 (63.51%), Postives = 57/74 (77.03%), Query Frame = 0 

Query: 82  GGVSRSSGP--ADSKRSDDGLGVDISAIRSNTVRLIDAQQNMVGIVSKAQAIQMADDAEL 141
           GG SR  G      K S+D   +DISAIRS TVRLID QQNM+G+VSK +A++ A+DAEL
Sbjct: 64  GGGSRFPGDRRGRQKESEDDDSLDISAIRSATVRLIDDQQNMIGLVSKEEAVRRAEDAEL 123

Query: 142 DLVVLSPDADPPVL 154
           DLV+LSPDADPPV+
Sbjct: 124 DLVILSPDADPPVV 137


HSP 2 Score: 54.3 bits (129), Expect = 1.3e-07
Identity = 28/47 (59.57%), Postives = 35/47 (74.47%), Query Frame = 0 

Query: 153 LATEEAKNLRDRNMFIVLAPNKAVLQKAQE-PPKVKDNLVVNEVSAS 199
           L TEE+KN RDRN+FIVL PNK V++K QE PPK K     ++VSA+
Sbjct: 227 LGTEESKNFRDRNLFIVLVPNKEVIRKVQEPPPKKKKKPADDKVSAA 273

[Close]
BLAST of Carg16428 vs. TAIR 10
Match: AT2G24060.1 (Translation initiation factor 3 protein )

HSP 1 Score: 81.6 bits (200), Expect = 7.6e-16
Identity = 43/74 (58.11%), Postives = 59/74 (79.73%), Query Frame = 0 

Query: 80  QYGGVSRSSGPADSKRSDDGLGVDISAIRSNTVRLIDAQQNMVGIVSKAQAIQMADDAEL 139
           ++ G +R   P +++  +    +DIS+IRS TVRLID QQNM+G+VSK +A++MADDAEL
Sbjct: 71  RFSGDNRRGRPKEAEIDE---ALDISSIRSATVRLIDGQQNMLGLVSKDEAVRMADDAEL 130

Query: 140 DLVVLSPDADPPVL 154
           DLV+LSPDADPPV+
Sbjct: 131 DLVILSPDADPPVV 141


HSP 2 Score: 58.2 bits (139), Expect = 9.0e-09
Identity = 29/47 (61.70%), Postives = 35/47 (74.47%), Query Frame = 0 

Query: 153 LATEEAKNLRDRNMFIVLAPNKAVLQKAQEPP-KVKDNLVVNEVSAS 199
           LATEE+KN RDRNMFI+L PNK +++K QEPP + K     NE SAS
Sbjct: 231 LATEESKNFRDRNMFIILVPNKEMIRKPQEPPTRKKKKTAENEASAS 277

The following BLAST results are available for this feature:
BLAST of Carg16428 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7020824.11.4e-96100.00Translation initiation factor IF3-2, chloroplastic [Cucurbita argyrosperma subsp... [more]
XP_022937979.13.1e-8065.98translation initiation factor IF3-4, chloroplastic-like [Cucurbita moschata][more]
KAG6586047.12.7e-7965.98Translation initiation factor IF3-2, chloroplastic, partial [Cucurbita argyrospe... [more]
XP_023536766.13.8e-7864.95translation initiation factor IF3-4, chloroplastic-like [Cucurbita pepo subsp. p... [more]
XP_022969585.18.0e-7664.16translation initiation factor IF3-4, chloroplastic-like [Cucurbita maxima][more]
BLAST of Carg16428 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q94B522.8e-1563.51Translation initiation factor IF3-4, chloroplastic OS=Arabidopsis thaliana OX=37... [more]
O822341.1e-1458.11Translation initiation factor IF3-2, chloroplastic OS=Arabidopsis thaliana OX=37... [more]
Q9CN422.0e-0558.70Translation initiation factor IF-3 OS=Pasteurella multocida (strain Pm70) OX=272... [more]
Q74IC43.5e-0556.52Translation initiation factor IF-3 OS=Lactobacillus johnsonii (strain CNCM I-122... [more]
Q8XJ671.3e-0454.35Translation initiation factor IF-3 OS=Clostridium perfringens (strain 13 / Type ... [more]
BLAST of Carg16428 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FCR31.5e-8065.98Translation initiation factor IF-3 OS=Cucurbita moschata OX=3662 GN=LOC111444202... [more]
A0A6J1I0C03.9e-7664.16Translation initiation factor IF-3 OS=Cucurbita maxima OX=3661 GN=LOC111468564 P... [more]
A0A6J1DYM54.8e-5853.08Translation initiation factor IF-3 OS=Momordica charantia OX=3673 GN=LOC11102471... [more]
A0A1S3BV821.2e-5350.17Translation initiation factor IF-3 OS=Cucumis melo OX=3656 GN=LOC103493630 PE=3 ... [more]
A0A6J1G3Y54.3e-5150.34Translation initiation factor IF-3 OS=Cucurbita moschata OX=3662 GN=LOC111450575... [more]
BLAST of Carg16428 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G30690.21.1e-2241.21Translation initiation factor 3 protein [more]
AT4G30690.12.0e-1663.51Translation initiation factor 3 protein [more]
AT2G24060.17.6e-1658.11Translation initiation factor 3 protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019814Translation initiation factor 3, N-terminalPFAMPF05198IF3_Ncoord: 106..153
e-value: 1.3E-13
score: 51.0
IPR036787Translation initiation factor 3 (IF-3), N-terminal domain superfamilyGENE3D3.10.20.80coord: 105..164
e-value: 8.9E-15
score: 56.5
IPR036787Translation initiation factor 3 (IF-3), N-terminal domain superfamilySUPERFAMILY54364Translation initiation factor IF3, N-terminal domaincoord: 106..153
IPR001288Translation initiation factor 3PANTHERPTHR10938TRANSLATION INITIATION FACTOR IF-3coord: 50..153
IPR001288Translation initiation factor 3PANTHERPTHR10938TRANSLATION INITIATION FACTOR IF-3coord: 153..196
NoneNo IPR availablePANTHERPTHR10938:SF6TRANSLATION INITIATION FACTOR IF-3coord: 153..196
NoneNo IPR availablePANTHERPTHR10938:SF6TRANSLATION INITIATION FACTOR IF-3coord: 50..153

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16428-RACarg16428-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006413 translational initiation
molecular_function GO:0003743 translation initiation factor activity