Carg16394 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg16394
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionU-box domain-containing protein 4-like
LocationCarg_Chr12: 3592140 .. 3593561 (-)
RNA-Seq ExpressionCarg16394
SyntenyCarg16394
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCGTTTCCGGCCATGGGGACGGACAGTCCCGCTAATTTTACTTATATGGGCCGGACCTTTGATGATCTCACCAACGGTCATAGTTCTGGGGCGTTTAGCGACTGTAATAGTGATAGATCCGGGGAATTTCCCACGGCGGCGTCGCAGGGCCGTCGGCTTTTGATTGCTTGTGCTTCTGATAACTCCGACGACCTAATTCGCCAACTGGTTATGGACCTCGAGTCCTGTTCGATTGAGGAGCAGAAGCAAGCGGCTATGGAGATTAGACTTCTCGCTAAGAACAAACCGGAGAATCGATTGAAGATCGCGAAAGCTGGAGCGGTGAGGCCGTTGATTTCGTTGATATCGTGTTCGGATCCGCAGCTTCAAGAGTACGGCGTGACGGCGATTTTGAATCTCTCGTTATGCGATGAGAACAAGGAGCAAATAGCGGCGTCTGGTGCGATTAAGCCTCTGGTTCGAGCGCTGATGTCGGGAACTCCTACGGCGAAGGAGAACGCCGCTTGTGCTTTACTACGACTATCGCAAATGGAGGAGAACAAGATTGCAATCGGACGGTGCGGAGCGATTCCGCTTTTGGTGAATCTGTTAGAGAACGGCGGATTTCGCGGAAAGAAGGACGCATCGACGGCTCTGTATTCACTGTGCTCGGTTAAGGAAAACAAAATCAGAGCAGTAAAAGCAGGAATCATGAGGCCGTTAGTGGAATTAATGGCGGATTTCGGGTCAAACATGGTGGATAAGTCGGCGTTCGTGTTGAGCGTATTGGTATCGTTGTCAGAGGCGAGGACGGCGCTGGTAGAAGAAGGCGGAATTCCGGTACTAGTGGAATTAGTTGAAGACGGAACACAGCGACAAAAGGAGATCGCGGCGGTGATTTTGCTGCAGGTTTGCGAGGACAGTGTCCTTTACCGTACAATGGTAGCCCGTGAAGGAGCAATCCCGCCGCTAATCGCCTTGACACAGTCCGGCACCAATCGCGCCAAACAAAAGGTACGATAATATTCCGGCCAAAACCTGCCTCTGACATTCTCAGGCCGACCAATTGGCATAACTGACGTGGCATTTCATTTTTTCCTAATTAAAAAAAAAAAAAAAAAAAAAAAAGAGAAGGCTGTCCTGCCACGTCAGTCTTCTTCTCTGGTTTTATCATTTTAATTCTTTTTAAAATCGAATTAAATTGAGAATTAGTAGTAATTAAAAAAATTTGTATTTATTGTGGGTGAAATTGAGGACAGGCGGAGAAACTGATAGAGCTTCTACGGCAACCAAGATCCGGCAACTGCGCTGCCACCACCTCAGATGTGTCAGTCTAATTTTTGTTGTGGCCACGTGGACGATGACGATGCAGACCCCCATACCCAAAAAAACAAATAGAGGAGAGGTTTTCTTGTAAATATATAATTTCTTTTTTTTTCTTT

mRNA sequence

TTCGTTTCCGGCCATGGGGACGGACAGTCCCGCTAATTTTACTTATATGGGCCGGACCTTTGATGATCTCACCAACGGTCATAGTTCTGGGGCGTTTAGCGACTGTAATAGTGATAGATCCGGGGAATTTCCCACGGCGGCGTCGCAGGGCCGTCGGCTTTTGATTGCTTGTGCTTCTGATAACTCCGACGACCTAATTCGCCAACTGGTTATGGACCTCGAGTCCTGTTCGATTGAGGAGCAGAAGCAAGCGGCTATGGAGATTAGACTTCTCGCTAAGAACAAACCGGAGAATCGATTGAAGATCGCGAAAGCTGGAGCGGTGAGGCCGTTGATTTCGTTGATATCGTGTTCGGATCCGCAGCTTCAAGAGTACGGCGTGACGGCGATTTTGAATCTCTCGTTATGCGATGAGAACAAGGAGCAAATAGCGGCGTCTGGTGCGATTAAGCCTCTGGTTCGAGCGCTGATGTCGGGAACTCCTACGGCGAAGGAGAACGCCGCTTGTGCTTTACTACGACTATCGCAAATGGAGGAGAACAAGATTGCAATCGGACGGTGCGGAGCGATTCCGCTTTTGGTGAATCTGTTAGAGAACGGCGGATTTCGCGGAAAGAAGGACGCATCGACGGCTCTGTATTCACTGTGCTCGGTTAAGGAAAACAAAATCAGAGCAGTAAAAGCAGGAATCATGAGGCCGTTAGTGGAATTAATGGCGGATTTCGGGTCAAACATGGTGGATAAGTCGGCGTTCGTGTTGAGCGTATTGGTATCGTTGTCAGAGGCGAGGACGGCGCTGGTAGAAGAAGGCGGAATTCCGGTACTAGTGGAATTAGTTGAAGACGGAACACAGCGACAAAAGGAGATCGCGGCGGTGATTTTGCTGCAGGTTTGCGAGGACAGTGTCCTTTACCGTACAATGGTAGCCCGTGAAGGAGCAATCCCGCCGCTAATCGCCTTGACACAGTCCGGCACCAATCGCGCCAAACAAAAGGCGGAGAAACTGATAGAGCTTCTACGGCAACCAAGATCCGGCAACTGCGCTGCCACCACCTCAGATGTGTCAGTCTAATTTTTGTTGTGGCCACGTGGACGATGACGATGCAGACCCCCATACCCAAAAAAACAAATAGAGGAGAGGTTTTCTTGTAAATATATAATTTCTTTTTTTTTCTTT

Coding sequence (CDS)

ATGGGGACGGACAGTCCCGCTAATTTTACTTATATGGGCCGGACCTTTGATGATCTCACCAACGGTCATAGTTCTGGGGCGTTTAGCGACTGTAATAGTGATAGATCCGGGGAATTTCCCACGGCGGCGTCGCAGGGCCGTCGGCTTTTGATTGCTTGTGCTTCTGATAACTCCGACGACCTAATTCGCCAACTGGTTATGGACCTCGAGTCCTGTTCGATTGAGGAGCAGAAGCAAGCGGCTATGGAGATTAGACTTCTCGCTAAGAACAAACCGGAGAATCGATTGAAGATCGCGAAAGCTGGAGCGGTGAGGCCGTTGATTTCGTTGATATCGTGTTCGGATCCGCAGCTTCAAGAGTACGGCGTGACGGCGATTTTGAATCTCTCGTTATGCGATGAGAACAAGGAGCAAATAGCGGCGTCTGGTGCGATTAAGCCTCTGGTTCGAGCGCTGATGTCGGGAACTCCTACGGCGAAGGAGAACGCCGCTTGTGCTTTACTACGACTATCGCAAATGGAGGAGAACAAGATTGCAATCGGACGGTGCGGAGCGATTCCGCTTTTGGTGAATCTGTTAGAGAACGGCGGATTTCGCGGAAAGAAGGACGCATCGACGGCTCTGTATTCACTGTGCTCGGTTAAGGAAAACAAAATCAGAGCAGTAAAAGCAGGAATCATGAGGCCGTTAGTGGAATTAATGGCGGATTTCGGGTCAAACATGGTGGATAAGTCGGCGTTCGTGTTGAGCGTATTGGTATCGTTGTCAGAGGCGAGGACGGCGCTGGTAGAAGAAGGCGGAATTCCGGTACTAGTGGAATTAGTTGAAGACGGAACACAGCGACAAAAGGAGATCGCGGCGGTGATTTTGCTGCAGGTTTGCGAGGACAGTGTCCTTTACCGTACAATGGTAGCCCGTGAAGGAGCAATCCCGCCGCTAATCGCCTTGACACAGTCCGGCACCAATCGCGCCAAACAAAAGGCGGAGAAACTGATAGAGCTTCTACGGCAACCAAGATCCGGCAACTGCGCTGCCACCACCTCAGATGTGTCAGTCTAA

Protein sequence

MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDDLIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQEYGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLYRTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV
Homology
BLAST of Carg16394 vs. NCBI nr
Match: XP_022951022.1 (U-box domain-containing protein 4-like [Cucurbita moschata] >KAG7020606.1 U-box domain-containing protein 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 665.6 bits (1716), Expect = 2.3e-187
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60
           MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD
Sbjct: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60

Query: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120
           LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE
Sbjct: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120

Query: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300
           MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300

Query: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 353
           RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV
Sbjct: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 352

BLAST of Carg16394 vs. NCBI nr
Match: XP_023536752.1 (U-box domain-containing protein 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 664.5 bits (1713), Expect = 5.2e-187
Identity = 351/352 (99.72%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60
           MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD
Sbjct: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60

Query: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120
           LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE
Sbjct: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120

Query: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300
           MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300

Query: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 353
           RTMVAREGAIPPLIALTQSGTNRA+QKAEKLIELLRQPRSGNCAATTSDVSV
Sbjct: 301 RTMVAREGAIPPLIALTQSGTNRARQKAEKLIELLRQPRSGNCAATTSDVSV 352

BLAST of Carg16394 vs. NCBI nr
Match: KAG6585704.1 (U-box domain-containing protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 664.1 bits (1712), Expect = 6.8e-187
Identity = 351/352 (99.72%), Postives = 351/352 (99.72%), Query Frame = 0

Query: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60
           MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD
Sbjct: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60

Query: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120
           LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE
Sbjct: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120

Query: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GRCGAIPLLVNLLENGGFRGKKDASTALYSLCS KENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSAKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300
           MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300

Query: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 353
           RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV
Sbjct: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 352

BLAST of Carg16394 vs. NCBI nr
Match: XP_023002752.1 (U-box domain-containing protein 4-like [Cucurbita maxima])

HSP 1 Score: 658.7 bits (1698), Expect = 2.9e-185
Identity = 348/352 (98.86%), Postives = 350/352 (99.43%), Query Frame = 0

Query: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60
           MGT+SP NFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD
Sbjct: 1   MGTESPVNFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60

Query: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120
           LIRQLVMDL+SCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE
Sbjct: 61  LIRQLVMDLQSCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120

Query: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300
           MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300

Query: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 353
           RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLR PRSGNCAATTSDVSV
Sbjct: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRLPRSGNCAATTSDVSV 352

BLAST of Carg16394 vs. NCBI nr
Match: XP_004142891.1 (U-box domain-containing protein 4 [Cucumis sativus] >KGN62451.1 hypothetical protein Csa_018696 [Cucumis sativus])

HSP 1 Score: 631.3 bits (1627), Expect = 4.9e-177
Identity = 333/352 (94.60%), Postives = 345/352 (98.01%), Query Frame = 0

Query: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60
           MGT++P NFTYMGRTFDDL+NG SSGAFSDCNSDRSGEFPTA+SQ RRLLIACASDNSD+
Sbjct: 1   MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60

Query: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120
           LIR LV+DLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISC+DPQLQE
Sbjct: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120

Query: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKE IAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GR GAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300
           MVDKSAFVLSVLVS+SEAR+ALVEEGGIPVLVELVEDGTQRQKEIAAVILLQ+CEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY 300

Query: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 353
           RTMVAREGAIPPL+AL+QSGTNRAKQKAEKLIELLRQPRSGN AATTSDVSV
Sbjct: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 352

BLAST of Carg16394 vs. ExPASy Swiss-Prot
Match: O22193 (U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 SV=3)

HSP 1 Score: 248.8 bits (634), Expect = 9.0e-65
Identity = 145/303 (47.85%), Postives = 209/303 (68.98%), Query Frame = 0

Query: 46  GRRLLIACASDNSDDL------IRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIA 105
           G R++ A +++   DL      +++LV +L+S S++ Q+QA  E+RLLAK+  +NR+ I 
Sbjct: 521 GSRIVSAPSNETRRDLSEVETQVKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIG 580

Query: 106 KAGAVRPLISLISCSDPQLQEYGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTA 165
            +GA+  L+ L+  +D   QE  VTA+LNLS+ D NK+ IA +GAI+PL+  L +G+  A
Sbjct: 581 NSGAIVLLVELLYSTDSATQENAVTALLNLSINDNNKKAIADAGAIEPLIHVLENGSSEA 640

Query: 166 KENAACALLRLSQMEENKIAIGRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKI 225
           KEN+A  L  LS +EENKI IG+ GAI  LV+LL NG  RGKKDA+TAL++L   +ENK 
Sbjct: 641 KENSAATLFSLSVIEENKIKIGQSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKA 700

Query: 226 RAVKAGIMRPLVELMADFGSNMVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGT 285
             V++G +R L++LM D  + MVDK+  VL+ L ++ E R A+ +EGGIP+LVE+VE G+
Sbjct: 701 MIVQSGAVRYLIDLM-DPAAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELGS 760

Query: 286 QRQKEIAAVILLQVCEDSVLYRTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPR 343
            R KE AA  LLQ+  +S  +  MV +EGA+PPL+AL+QSGT RA++KA+ L+   R  R
Sbjct: 761 ARGKENAAAALLQLSTNSGRFCNMVLQEGAVPPLVALSQSGTPRAREKAQALLSYFRNQR 820

BLAST of Carg16394 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.6e-58
Identity = 133/285 (46.67%), Postives = 191/285 (67.02%), Query Frame = 0

Query: 54  ASDNSDDLIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISC 113
           +SD     +  L+  L + + E+Q+ AA E+RLLAK   +NR+ IA+AGA+  L+ L+S 
Sbjct: 339 SSDCDRTFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEAGAIPLLVELLSS 398

Query: 114 SDPQLQEYGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQM 173
            DP+ QE+ VTA+LNLS+ + NK  I  +GAI  +V  L +G+  A+ENAA  L  LS +
Sbjct: 399 PDPRTQEHSVTALLNLSINEGNKGAIVDAGAITDIVEVLKNGSMEARENAAATLFSLSVI 458

Query: 174 EENKIAIGRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVEL 233
           +ENK+AIG  GAI  L++LLE G  RGKKDA+TA+++LC  + NK RAVK GI+ PL  L
Sbjct: 459 DENKVAIGAAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLTRL 518

Query: 234 MADFGSNMVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQV 293
           + D G  MVD++  +L++L +  E +TA+ E   IPVLVE++  G+ R +E AA IL  +
Sbjct: 519 LKDAGGGMVDEALAILAILSTNQEGKTAIAEAESIPVLVEIIRTGSPRNRENAAAILWYL 578

Query: 294 CEDSVLYRTMVARE-GAIPPLIALTQSGTNRAKQKAEKLIELLRQ 338
           C  ++  R  VARE GA   L  LT++GT+RAK+KA  L+EL++Q
Sbjct: 579 CIGNI-ERLNVAREVGADVALKELTENGTDRAKRKAASLLELIQQ 622

BLAST of Carg16394 vs. ExPASy Swiss-Prot
Match: Q5XEZ8 (U-box domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUB2 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.6e-58
Identity = 134/290 (46.21%), Postives = 190/290 (65.52%), Query Frame = 0

Query: 54  ASDNSDDLIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISC 113
           +S + +  +++L+ DL+S S++ Q++A   IR+LA+N  +NR+ IA+  A+  L+SL+  
Sbjct: 415 SSSSIETEVKKLIDDLKSSSLDTQREATARIRILARNSTDNRIVIARCEAIPSLVSLLYS 474

Query: 114 SDPQLQEYGVTAILNLSLCDENKEQIAASGAIKPLVRALMSG-TPTAKENAACALLRLSQ 173
           +D ++Q   VT +LNLS+ D NK  IA SGAI PL+  L +G    AK N+A  L  LS 
Sbjct: 475 TDERIQADAVTCLLNLSINDNNKSLIAESGAIVPLIHVLKTGYLEEAKANSAATLFSLSV 534

Query: 174 MEENKIAIGRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVE 233
           +EE K  IG  GAI  LV+LL +G   GKKDA+TAL++L    ENK + ++AG +R LVE
Sbjct: 535 IEEYKTEIGEAGAIEPLVDLLGSGSLSGKKDAATALFNLSIHHENKTKVIEAGAVRYLVE 594

Query: 234 LMADFGSNMVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQ 293
           LM D    MV+K+  VL+ L ++ E + A+ EEGGIPVLVE+VE G+ R KE A   LLQ
Sbjct: 595 LM-DPAFGMVEKAVVVLANLATVREGKIAIGEEGGIPVLVEVVELGSARGKENATAALLQ 654

Query: 294 VCEDSVLYRTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGN 343
           +C  S  +   V REG IPPL+ALT+SGT R K+KA+ L++  +  R  N
Sbjct: 655 LCTHSPKFCNNVIREGVIPPLVALTKSGTARGKEKAQNLLKYFKAHRQSN 703

BLAST of Carg16394 vs. ExPASy Swiss-Prot
Match: Q8GWV5 (U-box domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUB3 PE=2 SV=2)

HSP 1 Score: 226.1 bits (575), Expect = 6.2e-58
Identity = 136/277 (49.10%), Postives = 188/277 (67.87%), Query Frame = 0

Query: 64  QLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQEYGV 123
           +LV DL+S S + +  AA EIR L  N  ENR+ I + GA+ PL+SL+   +   QE+ V
Sbjct: 476 KLVEDLKSGSNKVKTAAAAEIRHLTINSIENRVHIGRCGAITPLLSLLYSEEKLTQEHAV 535

Query: 124 TAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGRC 183
           TA+LNLS+ + NK  I   GAI+PLV  L +G   AKEN+A +L  LS ++ N+  IG+ 
Sbjct: 536 TALLNLSISELNKAMIVEVGAIEPLVHVLNTGNDRAKENSAASLFSLSVLQVNRERIGQS 595

Query: 184 -GAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMV 243
             AI  LVNLL  G FRGKKDA++AL++L    +NK R V+A  ++ LVEL+ D    MV
Sbjct: 596 NAAIQALVNLLGKGTFRGKKDAASALFNLSITHDNKARIVQAKAVKYLVELL-DPDLEMV 655

Query: 244 DKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLYRT 303
           DK+  +L+ L ++ E R A+V EGGIP+LVE V+ G+QR KE AA +LLQ+C +S  + T
Sbjct: 656 DKAVALLANLSAVGEGRQAIVREGGIPLLVETVDLGSQRGKENAASVLLQLCLNSPKFCT 715

Query: 304 MVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPR 340
           +V +EGAIPPL+AL+QSGT RAK+KA++L+   R  R
Sbjct: 716 LVLQEGAIPPLVALSQSGTQRAKEKAQQLLSHFRNQR 751

BLAST of Carg16394 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 219.9 bits (559), Expect = 4.5e-56
Identity = 128/284 (45.07%), Postives = 189/284 (66.55%), Query Frame = 0

Query: 57  NSDDL--IRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCS 116
           NS D+  IR LV  L S S E+++ A  EIR L+K   +NR+ IA+AGA+  L++L++  
Sbjct: 326 NSGDMSVIRALVQRLSSRSTEDRRNAVSEIRSLSKRSTDNRILIAEAGAIPVLVNLLTSE 385

Query: 117 DPQLQEYGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQME 176
           D   QE  +T +LNLS+ + NKE I  +GA+  +V+ L +GT  A+ENAA  L  LS  +
Sbjct: 386 DVATQENAITCVLNLSIYENNKELIMFAGAVTSIVQVLRAGTMEARENAAATLFSLSLAD 445

Query: 177 ENKIAIGRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELM 236
           ENKI IG  GAIP LV+LLENG  RGKKDA+TAL++LC    NK RAV+AGI+  LV+++
Sbjct: 446 ENKIIIGGSGAIPALVDLLENGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKML 505

Query: 237 ADFGSN-MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQV 296
           +D   + MVD++  +LSVL +  +A++A+V+   +P L+ +++    R +E AA ILL +
Sbjct: 506 SDSTRHRMVDEALTILSVLANNQDAKSAIVKANTLPALIGILQTDQTRNRENAAAILLSL 565

Query: 297 CEDSVLYRTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQ 338
           C+        + R GA+ PL+ L+++GT R K+KA  L+ELLR+
Sbjct: 566 CKRDTEKLITIGRLGAVVPLMDLSKNGTERGKRKAISLLELLRK 609

BLAST of Carg16394 vs. ExPASy TrEMBL
Match: A0A6J1GGH6 (U-box domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111453995 PE=4 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.1e-187
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60
           MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD
Sbjct: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60

Query: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120
           LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE
Sbjct: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120

Query: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300
           MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300

Query: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 353
           RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV
Sbjct: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 352

BLAST of Carg16394 vs. ExPASy TrEMBL
Match: A0A6J1KKD4 (U-box domain-containing protein 4-like OS=Cucurbita maxima OX=3661 GN=LOC111496516 PE=4 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.4e-185
Identity = 348/352 (98.86%), Postives = 350/352 (99.43%), Query Frame = 0

Query: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60
           MGT+SP NFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD
Sbjct: 1   MGTESPVNFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60

Query: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120
           LIRQLVMDL+SCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE
Sbjct: 61  LIRQLVMDLQSCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120

Query: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300
           MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300

Query: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 353
           RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLR PRSGNCAATTSDVSV
Sbjct: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRLPRSGNCAATTSDVSV 352

BLAST of Carg16394 vs. ExPASy TrEMBL
Match: A0A0A0LR48 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354770 PE=4 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.4e-177
Identity = 333/352 (94.60%), Postives = 345/352 (98.01%), Query Frame = 0

Query: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60
           MGT++P NFTYMGRTFDDL+NG SSGAFSDCNSDRSGEFPTA+SQ RRLLIACASDNSD+
Sbjct: 1   MGTENPMNFTYMGRTFDDLSNGDSSGAFSDCNSDRSGEFPTASSQSRRLLIACASDNSDE 60

Query: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120
           LIR LV+DLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISC+DPQLQE
Sbjct: 61  LIRHLVLDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQE 120

Query: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSLCDENKE IAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GR GAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRSGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300
           MVDKSAFVLSVLVS+SEAR+ALVEEGGIPVLVELVEDGTQRQKEIAAVILLQ+CEDSVLY
Sbjct: 241 MVDKSAFVLSVLVSMSEARSALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLY 300

Query: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 353
           RTMVAREGAIPPL+AL+QSGTNRAKQKAEKLIELLRQPRSGN AATTSDVSV
Sbjct: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 352

BLAST of Carg16394 vs. ExPASy TrEMBL
Match: A0A1S3BBF2 (U-box domain-containing protein 4 OS=Cucumis melo OX=3656 GN=LOC103487841 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 6.0e-173
Identity = 326/345 (94.49%), Postives = 337/345 (97.68%), Query Frame = 0

Query: 8   NFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDDLIRQLVM 67
           NFTYMGR+FDDL+NG SSGAFSDCNSDRSGEFPT +SQ RRLLIACASDNSD+LIR LV+
Sbjct: 2   NFTYMGRSFDDLSNGDSSGAFSDCNSDRSGEFPTGSSQSRRLLIACASDNSDELIRHLVL 61

Query: 68  DLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQEYGVTAIL 127
           DLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISC+DPQLQEYGVTAIL
Sbjct: 62  DLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCTDPQLQEYGVTAIL 121

Query: 128 NLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGRCGAIP 187
           NLSLCDENKE IAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGR GAIP
Sbjct: 122 NLSLCDENKELIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGRSGAIP 181

Query: 188 LLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMVDKSAF 247
           LLVNLLE+GGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMVDKSAF
Sbjct: 182 LLVNLLESGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMVDKSAF 241

Query: 248 VLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLYRTMVARE 307
           VLSVLVS+ EARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQ+CEDSVLYRTMVARE
Sbjct: 242 VLSVLVSMPEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQICEDSVLYRTMVARE 301

Query: 308 GAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 353
           GAIPPL+AL+QSGTNRAKQKAEKLIELLRQPRSGN AATTSDVSV
Sbjct: 302 GAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSDVSV 346

BLAST of Carg16394 vs. ExPASy TrEMBL
Match: A0A6J1HEZ8 (U-box domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111462862 PE=4 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 9.6e-171
Identity = 322/352 (91.48%), Postives = 341/352 (96.88%), Query Frame = 0

Query: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60
           MGT++P NF+YMGR+FDDLTNG SSGAFSDCNSDRSGEFP A+SQ RRLLI+CASDNSD+
Sbjct: 1   MGTENPVNFSYMGRSFDDLTNGDSSGAFSDCNSDRSGEFPMASSQSRRLLISCASDNSDE 60

Query: 61  LIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQE 120
           LI+QLV+DLESCSI+EQKQAAMEIRLLAKNKPENRL+IAKAGAVRPLISLISC+DPQLQE
Sbjct: 61  LIQQLVLDLESCSIDEQKQAAMEIRLLAKNKPENRLRIAKAGAVRPLISLISCTDPQLQE 120

Query: 121 YGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAI 180
           YGVTAILNLSL DENKE IAASGAIKPLVRALMSGT TAKENAACALLRLSQMEENKIAI
Sbjct: 121 YGVTAILNLSLNDENKELIAASGAIKPLVRALMSGTSTAKENAACALLRLSQMEENKIAI 180

Query: 181 GRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240
           GR GAIPLLVNLL NGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN
Sbjct: 181 GRSGAIPLLVNLLGNGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSN 240

Query: 241 MVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLY 300
           MVDKSAFVLSVLVS+SEARTALV+EGGIPVLVELVEDGTQRQKEIAAVILLQ+CE+SVLY
Sbjct: 241 MVDKSAFVLSVLVSMSEARTALVDEGGIPVLVELVEDGTQRQKEIAAVILLQICEESVLY 300

Query: 301 RTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRSGNCAATTSDVSV 353
           RTMVAREGAIPPL+AL+QSGTNRAKQKAEKLIELLRQPRSGN AATTS+V V
Sbjct: 301 RTMVAREGAIPPLVALSQSGTNRAKQKAEKLIELLRQPRSGNYAATTSNVCV 352

BLAST of Carg16394 vs. TAIR 10
Match: AT3G01400.1 (ARM repeat superfamily protein )

HSP 1 Score: 515.8 bits (1327), Expect = 2.8e-146
Identity = 271/341 (79.47%), Postives = 307/341 (90.03%), Query Frame = 0

Query: 1   MGTDSPANFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQGRRLLIACASDNSDD 60
           M    P +FTYMGR F DL+    S AFSDCNSDRSGEFPTA+S+ RRLL++CAS+NSDD
Sbjct: 3   MENHRPGSFTYMGRKFSDLSLNDDSSAFSDCNSDRSGEFPTASSESRRLLLSCASENSDD 62

Query: 61  LIRQLVMDLESC-SIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQ 120
           LI  LV  L+S  SI+EQKQAAMEIRLL+KNKPENR+KIAKAGA++PLISLIS SD QLQ
Sbjct: 63  LINHLVSHLDSSYSIDEQKQAAMEIRLLSKNKPENRIKIAKAGAIKPLISLISSSDLQLQ 122

Query: 121 EYGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIA 180
           EYGVTAILNLSLCDENKE IA+SGAIKPLVRAL  GTPTAKENAACALLRLSQ+EENK+A
Sbjct: 123 EYGVTAILNLSLCDENKESIASSGAIKPLVRALKMGTPTAKENAACALLRLSQIEENKVA 182

Query: 181 IGRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGS 240
           IGR GAIPLLVNLLE GGFR KKDASTALYSLCS KENKIRAV++GIM+PLVELMADFGS
Sbjct: 183 IGRSGAIPLLVNLLETGGFRAKKDASTALYSLCSAKENKIRAVQSGIMKPLVELMADFGS 242

Query: 241 NMVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVL 300
           NMVDKSAFV+S+L+S+ E++ A+VEEGG+PVLVE+VE GTQRQKE+A  ILLQ+CE+SV+
Sbjct: 243 NMVDKSAFVMSLLMSVPESKPAIVEEGGVPVLVEIVEVGTQRQKEMAVSILLQLCEESVV 302

Query: 301 YRTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPRS 341
           YRTMVAREGAIPPL+AL+Q+GT+RAKQKAE LIELLRQPRS
Sbjct: 303 YRTMVAREGAIPPLVALSQAGTSRAKQKAEALIELLRQPRS 343

BLAST of Carg16394 vs. TAIR 10
Match: AT5G58680.1 (ARM repeat superfamily protein )

HSP 1 Score: 461.5 bits (1186), Expect = 6.2e-130
Identity = 245/336 (72.92%), Postives = 286/336 (85.12%), Query Frame = 0

Query: 8   NFTYMGRTFDDLTNGHSSGAFSDCNSDRSGEFPTAASQG--RRLLIACASDNSDDLIRQL 67
           +FTYM  +F D++    S AFSDCNSD S EFPT  S+   R+L ++CA DNSDD+IR L
Sbjct: 6   SFTYMDHSFSDISLNFDSSAFSDCNSDISCEFPTTTSESRQRKLFLSCAVDNSDDVIRNL 65

Query: 68  VMDLE-SCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLISLISCSDPQLQEYGVT 127
           +  LE S SIEEQKQAAMEIRLL+KNKPENR+K+AKAGA++PL+SLIS SD QLQEYGVT
Sbjct: 66  ITHLESSSSIEEQKQAAMEIRLLSKNKPENRIKLAKAGAIKPLVSLISSSDLQLQEYGVT 125

Query: 128 AILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALLRLSQMEENKIAIGRCG 187
           A+LNLSLCDENKE I +SGA+KPLV AL  GTPT KENAACALLRLSQ+EENKI IGR G
Sbjct: 126 AVLNLSLCDENKEMIVSSGAVKPLVNALRLGTPTTKENAACALLRLSQVEENKITIGRSG 185

Query: 188 AIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMRPLVELMADFGSNMVDK 247
           AIPLLVNLLENGGFR KKDASTALYSLCS  ENK RAV++GIM+PLVELM DF S+MVDK
Sbjct: 186 AIPLLVNLLENGGFRAKKDASTALYSLCSTNENKTRAVESGIMKPLVELMIDFESDMVDK 245

Query: 248 SAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAVILLQVCEDSVLYRTMV 307
           SAFV+++L+S  E++ A+VEEGG+PVLVE+VE GTQRQKEI+  ILLQ+CE+SV+YRTMV
Sbjct: 246 SAFVMNLLMSAPESKPAVVEEGGVPVLVEIVEAGTQRQKEISVSILLQLCEESVVYRTMV 305

Query: 308 AREGAIPPLIALTQSGTNR-AKQKAEKLIELLRQPR 340
           AREGA+PPL+AL+Q   +R AK KAE LIELLRQPR
Sbjct: 306 AREGAVPPLVALSQGSASRGAKVKAEALIELLRQPR 341

BLAST of Carg16394 vs. TAIR 10
Match: AT4G16490.1 (ARM repeat superfamily protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.8e-76
Identity = 154/293 (52.56%), Postives = 215/293 (73.38%), Query Frame = 0

Query: 50  LIACAS-DNSDDLIRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIAKAGAVRPLI 109
           +I C S ++    ++  +  L S S+  ++ AA ++RLLAKN+ +NR+ I ++GA++ LI
Sbjct: 172 IIECISPEDLQPTVKLCIDGLRSSSVAIKRSAAAKLRLLAKNRADNRVLIGESGAIQALI 231

Query: 110 SLISCSDPQLQEYGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTAKENAACALL 169
            L+ C+DP  QE+ VTA+LNLSL D+NK  IAA GAIK LV  L +GT T+K+NAACALL
Sbjct: 232 PLLRCNDPWTQEHAVTALLNLSLHDQNKAVIAAGGAIKSLVWVLKTGTETSKQNAACALL 291

Query: 170 RLSQMEENKIAIGRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKIRAVKAGIMR 229
            L+ +EENK +IG CGAIP LV+LL NG  RGKKDA T LY LC++++NK RAV AG ++
Sbjct: 292 SLALLEENKGSIGACGAIPPLVSLLLNGSCRGKKDALTTLYKLCTLQQNKERAVTAGAVK 351

Query: 230 PLVELMADFGSNMVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGTQRQKEIAAV 289
           PLV+L+A+ G+ M +K+  VLS L ++ + + A+VEEGGI  LVE +EDG+ + KE A +
Sbjct: 352 PLVDLVAEEGTGMAEKAMVVLSSLAAIDDGKEAIVEEGGIAALVEAIEDGSVKGKEFAIL 411

Query: 290 ILLQVCEDSVLYRTMVAREGAIPPLIALTQSG--TNRAKQKAEKLIELLRQPR 340
            LLQ+C DSV  R ++ REGAIPPL+ L+QSG  + RAK+KAE+L+  LR+PR
Sbjct: 412 TLLQLCSDSVRNRGLLVREGAIPPLVGLSQSGSVSVRAKRKAERLLGYLREPR 464

BLAST of Carg16394 vs. TAIR 10
Match: AT2G23140.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 248.8 bits (634), Expect = 6.4e-66
Identity = 145/303 (47.85%), Postives = 209/303 (68.98%), Query Frame = 0

Query: 46  GRRLLIACASDNSDDL------IRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIA 105
           G R++ A +++   DL      +++LV +L+S S++ Q+QA  E+RLLAK+  +NR+ I 
Sbjct: 524 GSRIVSAPSNETRRDLSEVETQVKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIG 583

Query: 106 KAGAVRPLISLISCSDPQLQEYGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTA 165
            +GA+  L+ L+  +D   QE  VTA+LNLS+ D NK+ IA +GAI+PL+  L +G+  A
Sbjct: 584 NSGAIVLLVELLYSTDSATQENAVTALLNLSINDNNKKAIADAGAIEPLIHVLENGSSEA 643

Query: 166 KENAACALLRLSQMEENKIAIGRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKI 225
           KEN+A  L  LS +EENKI IG+ GAI  LV+LL NG  RGKKDA+TAL++L   +ENK 
Sbjct: 644 KENSAATLFSLSVIEENKIKIGQSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKA 703

Query: 226 RAVKAGIMRPLVELMADFGSNMVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGT 285
             V++G +R L++LM D  + MVDK+  VL+ L ++ E R A+ +EGGIP+LVE+VE G+
Sbjct: 704 MIVQSGAVRYLIDLM-DPAAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELGS 763

Query: 286 QRQKEIAAVILLQVCEDSVLYRTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPR 343
            R KE AA  LLQ+  +S  +  MV +EGA+PPL+AL+QSGT RA++KA+ L+   R  R
Sbjct: 764 ARGKENAAAALLQLSTNSGRFCNMVLQEGAVPPLVALSQSGTPRAREKAQALLSYFRNQR 823

BLAST of Carg16394 vs. TAIR 10
Match: AT2G23140.2 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 248.8 bits (634), Expect = 6.4e-66
Identity = 145/303 (47.85%), Postives = 209/303 (68.98%), Query Frame = 0

Query: 46  GRRLLIACASDNSDDL------IRQLVMDLESCSIEEQKQAAMEIRLLAKNKPENRLKIA 105
           G R++ A +++   DL      +++LV +L+S S++ Q+QA  E+RLLAK+  +NR+ I 
Sbjct: 521 GSRIVSAPSNETRRDLSEVETQVKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIG 580

Query: 106 KAGAVRPLISLISCSDPQLQEYGVTAILNLSLCDENKEQIAASGAIKPLVRALMSGTPTA 165
            +GA+  L+ L+  +D   QE  VTA+LNLS+ D NK+ IA +GAI+PL+  L +G+  A
Sbjct: 581 NSGAIVLLVELLYSTDSATQENAVTALLNLSINDNNKKAIADAGAIEPLIHVLENGSSEA 640

Query: 166 KENAACALLRLSQMEENKIAIGRCGAIPLLVNLLENGGFRGKKDASTALYSLCSVKENKI 225
           KEN+A  L  LS +EENKI IG+ GAI  LV+LL NG  RGKKDA+TAL++L   +ENK 
Sbjct: 641 KENSAATLFSLSVIEENKIKIGQSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKA 700

Query: 226 RAVKAGIMRPLVELMADFGSNMVDKSAFVLSVLVSLSEARTALVEEGGIPVLVELVEDGT 285
             V++G +R L++LM D  + MVDK+  VL+ L ++ E R A+ +EGGIP+LVE+VE G+
Sbjct: 701 MIVQSGAVRYLIDLM-DPAAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELGS 760

Query: 286 QRQKEIAAVILLQVCEDSVLYRTMVAREGAIPPLIALTQSGTNRAKQKAEKLIELLRQPR 343
            R KE AA  LLQ+  +S  +  MV +EGA+PPL+AL+QSGT RA++KA+ L+   R  R
Sbjct: 761 ARGKENAAAALLQLSTNSGRFCNMVLQEGAVPPLVALSQSGTPRAREKAQALLSYFRNQR 820

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022951022.12.3e-187100.00U-box domain-containing protein 4-like [Cucurbita moschata] >KAG7020606.1 U-box ... [more]
XP_023536752.15.2e-18799.72U-box domain-containing protein 4-like [Cucurbita pepo subsp. pepo][more]
KAG6585704.16.8e-18799.72U-box domain-containing protein 4, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023002752.12.9e-18598.86U-box domain-containing protein 4-like [Cucurbita maxima][more]
XP_004142891.14.9e-17794.60U-box domain-containing protein 4 [Cucumis sativus] >KGN62451.1 hypothetical pro... [more]
Match NameE-valueIdentityDescription
O221939.0e-6547.85U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 S... [more]
Q8VZ401.6e-5846.67U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
Q5XEZ81.6e-5846.21U-box domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUB2 PE=2 S... [more]
Q8GWV56.2e-5849.10U-box domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUB3 PE=2 S... [more]
Q8GUG94.5e-5645.07U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1GGH61.1e-187100.00U-box domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1KKD41.4e-18598.86U-box domain-containing protein 4-like OS=Cucurbita maxima OX=3661 GN=LOC1114965... [more]
A0A0A0LR482.4e-17794.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354770 PE=4 SV=1[more]
A0A1S3BBF26.0e-17394.49U-box domain-containing protein 4 OS=Cucumis melo OX=3656 GN=LOC103487841 PE=4 S... [more]
A0A6J1HEZ89.6e-17191.48U-box domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
Match NameE-valueIdentityDescription
AT3G01400.12.8e-14679.47ARM repeat superfamily protein [more]
AT5G58680.16.2e-13072.92ARM repeat superfamily protein [more]
AT4G16490.11.8e-7652.56ARM repeat superfamily protein [more]
AT2G23140.16.4e-6647.85RING/U-box superfamily protein with ARM repeat domain [more]
AT2G23140.26.4e-6647.85RING/U-box superfamily protein with ARM repeat domain [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 173..213
e-value: 0.13
score: 21.3
coord: 214..254
e-value: 20.0
score: 10.8
coord: 132..172
e-value: 0.0019
score: 27.5
coord: 91..131
e-value: 0.0016
score: 27.7
coord: 255..295
e-value: 6.3
score: 14.8
IPR000225ArmadilloPFAMPF00514Armcoord: 92..130
e-value: 6.0E-6
score: 26.1
coord: 174..213
e-value: 1.3E-6
score: 28.2
coord: 133..171
e-value: 4.0E-6
score: 26.7
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 102..144
score: 10.3074
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 202..348
e-value: 4.9E-22
score: 80.1
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 41..201
e-value: 3.2E-32
score: 113.9
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 1..345
NoneNo IPR availablePANTHERPTHR23315:SF313U-BOX DOMAIN-CONTAINING PROTEIN 4-LIKE ISOFORM X1coord: 1..345
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 59..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16394-RACarg16394-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding