Carg16304 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg16304
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionS-acyltransferase
LocationCarg_Chr19: 5674134 .. 5675794 (+)
RNA-Seq ExpressionCarg16304
SyntenyCarg16304
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGGAGAGTTTTTTGGAGTGTATATCCGTTTTTTCAATCTCTTTCAGAAACATTCCGTCAAATCCCACTGCTTAATTCTCGTCGATCCTTCTTCTTCAGTCTTCACTTCACGCCCGCCTTCGAAGATTCCATGGAACTTCTCTAATTTTCTCCATCTCCTCGCAGTAGTTGTCTCGACCCCCCCGTTCTTTCCCTGGTATTGAATCCATTTCTCTATCTTTTTTTTGTTTGTTTAACTGCAATTGTTTGCTTTCTCTTATGATTGATTTTTGGGAACTTCGCTGAATTATGGTAATTTGTTTCGAGTATCCAGAATTCGAAATGAAACTGGTGCGTCAAATTTATGTGGCTGTCGAATTGAGTTGTTTGTTTGTGTTGGCGGGTTCGTCTCAAATCGAATTGAGGAGTATTTTGTTGTGACTTGGGCTTATGGAATAGTTTGATCTGATTAAAGTGAAATATAGGTTAGTGAAGGTTTTGATGGAACTTTTAGGAATATGCGCTTATTTCACCGAAGACTACTGAATCTCTACTGGGAATTTTTGGAATGATTACTTGGGAACCGTTTGCAAAACTATATGATTGGCGTTTTCAAGAATTTTTGTTTAAATGGTTCTTATATCAATGATTGTTCGATTCTTTATTGTCCTTTTTTTCTATCAAACAGTGGAATCGTTCTGTTATGTTCTGCCCAAACTGATAGCTTCTATGGTTGAATGAGACCCATGATAATTGTACTCTGTTGATTATTAATTCTTCTCCATCTTTGCATCGATACCGTGCAGAGAAGGTTTTGTTTTGTCGTTTATATTTTCGTACACAAAATTTCACTCTTAGCTATTTTCCTTGGTACTTTAGAGTGCAATCAGCTATCTCCCTGGTCCCTTTTCAAGTTTTACCTCACATGGCTTCGTCTGAGATTGAGGTTGTAGCATCACCCAACTCAAAATCTCAGACAAACCCTGAAGTTGCCATTGTAGACGTTTACTCTGCTTCTGCTTATGGCGACTTCCAGAAAGTGAGGAGTTTCGTTGAAGAGCATGGAGCTTCTGTTTTGGTTCCTGATTCCAACGGGTACTATGCTTTGCAATGGGCTGCTTTAAACAACTTCCCTGATATCGTCCAGTACATCATTGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACTCTTTTTGCCTTCCTGATTTTAATGATATTATGTCTCTCTAGATCGTTGGATGTAGATTGATCGTCCCTGTTATATTGCATGTTTTGAGTTTAGTCAGTTGACTTGTTTTATGTAACGTAAAGCTGGCGTAGCGTGATTTATGTGTCGTTCCAGATAGAGGAAGAAGAATAAAATTTATTGGATGTGAAGGAAGTGCATGTGTTGGATTTTTGTATTGATGAATTTCATAGCATTAGGGCATTTTTTATGTTGCTCAAGAAGTTTAAATTGATGGTCTTTTGAGGTTTATGTCAGTTCCCTACCAAAGTTTTTGATGATTGATTTGATCAGCATGGAGCGGATGTCAATTCCTGTGACAAATTGCAGCAGACAGCGCTTCACTGGGCAGCAGTTCGAGGTTCAATTATGGCTGCAGACGTACTCCTGCTGAATGGAGCTCGAGTGGAGGCAGCTGATATAAATGGGTATCGGGTAACTTTGAATATTTAG

mRNA sequence

AAAGGAGAGTTTTTTGGAGTGTATATCCGTTTTTTCAATCTCTTTCAGAAACATTCCGTCAAATCCCACTGCTTAATTCTCGTCGATCCTTCTTCTTCAGTCTTCACTTCACGCCCGCCTTCGAAGATTCCATGGAACTTCTCTAATTTTCTCCATCTCCTCGCAGTAGTTGTCTCGACCCCCCCGTTCTTTCCCTGAGTGCAATCAGCTATCTCCCTGGTCCCTTTTCAAGTTTTACCTCACATGGCTTCGTCTGAGATTGAGGTTGTAGCATCACCCAACTCAAAATCTCAGACAAACCCTGAAGTTGCCATTGTAGACGTTTACTCTGCTTCTGCTTATGGCGACTTCCAGAAAGTGAGGAGTTTCGTTGAAGAGCATGGAGCTTCTGTTTTGGTTCCTGATTCCAACGGGTACTATGCTTTGCAATGGGCTGCTTTAAACAACTTCCCTGATATCGTCCAGTACATCATTGAGCATGGAGCGGATGTCAATTCCTGTGACAAATTGCAGCAGACAGCGCTTCACTGGGCAGCAGTTCGAGGTTCAATTATGGCTGCAGACGTACTCCTGCTGAATGGAGCTCGAGTGGAGGCAGCTGATATAAATGGGTATCGGGTAACTTTGAATATTTAG

Coding sequence (CDS)

ATGGCTTCGTCTGAGATTGAGGTTGTAGCATCACCCAACTCAAAATCTCAGACAAACCCTGAAGTTGCCATTGTAGACGTTTACTCTGCTTCTGCTTATGGCGACTTCCAGAAAGTGAGGAGTTTCGTTGAAGAGCATGGAGCTTCTGTTTTGGTTCCTGATTCCAACGGGTACTATGCTTTGCAATGGGCTGCTTTAAACAACTTCCCTGATATCGTCCAGTACATCATTGAGCATGGAGCGGATGTCAATTCCTGTGACAAATTGCAGCAGACAGCGCTTCACTGGGCAGCAGTTCGAGGTTCAATTATGGCTGCAGACGTACTCCTGCTGAATGGAGCTCGAGTGGAGGCAGCTGATATAAATGGGTATCGGGTAACTTTGAATATTTAG

Protein sequence

MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYALQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAADINGYRVTLNI
Homology
BLAST of Carg16304 vs. NCBI nr
Match: KAG7011580.1 (putative protein S-acyltransferase 23 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 258.1 bits (658), Expect = 4.2e-65
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0

Query: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60
           MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA
Sbjct: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60

Query: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120
           LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD
Sbjct: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120

Query: 121 INGYRVTLNI 131
           INGYRVTLNI
Sbjct: 121 INGYRVTLNI 130

BLAST of Carg16304 vs. NCBI nr
Match: XP_023511407.1 (probable protein S-acyltransferase 23 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 249.6 bits (636), Expect = 1.5e-62
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0

Query: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60
           MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA
Sbjct: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60

Query: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120
           LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD
Sbjct: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120

Query: 121 INGYR 126
           INGYR
Sbjct: 121 INGYR 125

BLAST of Carg16304 vs. NCBI nr
Match: XP_022972424.1 (probable protein S-acyltransferase 23 [Cucurbita maxima])

HSP 1 Score: 249.6 bits (636), Expect = 1.5e-62
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0

Query: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60
           MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA
Sbjct: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60

Query: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120
           LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD
Sbjct: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120

Query: 121 INGYR 126
           INGYR
Sbjct: 121 INGYR 125

BLAST of Carg16304 vs. NCBI nr
Match: XP_038888969.1 (probable protein S-acyltransferase 23 isoform X1 [Benincasa hispida] >XP_038888970.1 probable protein S-acyltransferase 23 isoform X1 [Benincasa hispida])

HSP 1 Score: 248.8 bits (634), Expect = 2.5e-62
Identity = 123/125 (98.40%), Postives = 125/125 (100.00%), Query Frame = 0

Query: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60
           MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGAS+LVPDSNGYYA
Sbjct: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASILVPDSNGYYA 60

Query: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120
           LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAAD+LLLNGARVEAAD
Sbjct: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADILLLNGARVEAAD 120

Query: 121 INGYR 126
           INGYR
Sbjct: 121 INGYR 125

BLAST of Carg16304 vs. NCBI nr
Match: XP_022952854.1 (probable protein S-acyltransferase 23 [Cucurbita moschata])

HSP 1 Score: 247.7 bits (631), Expect = 5.6e-62
Identity = 124/125 (99.20%), Postives = 124/125 (99.20%), Query Frame = 0

Query: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60
           MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHG SVLVPDSNGYYA
Sbjct: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGPSVLVPDSNGYYA 60

Query: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120
           LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD
Sbjct: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120

Query: 121 INGYR 126
           INGYR
Sbjct: 121 INGYR 125

BLAST of Carg16304 vs. ExPASy Swiss-Prot
Match: Q3EC11 (Probable protein S-acyltransferase 23 OS=Arabidopsis thaliana OX=3702 GN=PAT23 PE=2 SV=2)

HSP 1 Score: 154.1 bits (388), Expect = 1.1e-36
Identity = 76/126 (60.32%), Postives = 93/126 (73.81%), Query Frame = 0

Query: 1   MASSEIEVV-ASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYY 60
           M SSEIEVV    NS         I DV+SASAYGD  +++ FVE +G+SV +PD NG+Y
Sbjct: 1   MDSSEIEVVPLDSNSHQSPTESPTITDVFSASAYGDLHQLKHFVEHNGSSVSLPDDNGFY 60

Query: 61  ALQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAA 120
           ALQWAALNN   + QYII+HG DVNS D +QQT LHWAAV+GSI  AD+LL +GAR+EA 
Sbjct: 61  ALQWAALNNSLHVAQYIIQHGGDVNSADNIQQTPLHWAAVKGSIDVADLLLQHGARIEAV 120

Query: 121 DINGYR 126
           D+NG+R
Sbjct: 121 DVNGFR 126

BLAST of Carg16304 vs. ExPASy Swiss-Prot
Match: Q52T38 (Protein S-acyltransferase 24 OS=Arabidopsis thaliana OX=3702 GN=PAT24 PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 3.5e-30
Identity = 70/134 (52.24%), Postives = 89/134 (66.42%), Query Frame = 0

Query: 3   SSEIEVVASPNSKSQTNPEVAIV---------DVYSASAYGDFQKVRSFVEEHGASVLVP 62
           SSEIEVV    S  + N E +           DVY+A+AYGD +K+   VE  G+SV  P
Sbjct: 2   SSEIEVVEEIQSNPKENGESSSKGIEEESLKNDVYTAAAYGDLEKLHRLVECEGSSVSEP 61

Query: 63  DSNGYYALQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNG 122
           D+ GYYALQW+ALNN   + QY+IEHG DVN+ D   QTALHW+AVRG+I  A++LL  G
Sbjct: 62  DALGYYALQWSALNNRVAVAQYLIEHGGDVNATDHTGQTALHWSAVRGAIQVAELLLQEG 121

Query: 123 ARVEAADINGYRVT 128
           ARV+A D+ GY+ T
Sbjct: 122 ARVDATDMYGYQAT 135

BLAST of Carg16304 vs. ExPASy Swiss-Prot
Match: Q9VUW9 (Palmitoyltransferase Hip14 OS=Drosophila melanogaster OX=7227 GN=Hip14 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 4.7e-11
Identity = 41/117 (35.04%), Postives = 60/117 (51.28%), Query Frame = 0

Query: 8   VVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYALQWAALN 67
           ++A     +   P+ +  D+  A+ YG   +VR  VE  G  V  PDS     L WAA+N
Sbjct: 30  LIAQQPPTAPVEPDYSGFDIVKATQYGAIARVRELVES-GWDVNQPDSETVTLLHWAAIN 89

Query: 68  NFPDIVQYIIEHGADVNSC-DKLQQTALHWAAVRGSIMAADVLLLNGARVEAADING 124
           N  DI++Y +E GA V++   +L  T LHWA  +G + A  +L+  GA     D  G
Sbjct: 90  NRRDIIRYFLEKGATVDAVGGELNATPLHWATRQGHLGAVVLLMAAGADPRIRDAEG 145

BLAST of Carg16304 vs. ExPASy Swiss-Prot
Match: Q09701 (Palmitoyltransferase akr1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=akr1 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 8.0e-11
Identity = 35/99 (35.35%), Postives = 53/99 (53.54%), Query Frame = 0

Query: 27  VYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYALQWAALNNFPDIVQYIIEHGADVNSC 86
           ++ A++ G+   V++ +      V   D  G  AL WAALN    I ++++EHGADVN+ 
Sbjct: 4   LFLAASQGELDTVKNLISSEKIDVNATDEGGATALHWAALNQQIPICKFLLEHGADVNAI 63

Query: 87  -DKLQQTALHWAAVRGSIMAADVLLLNGARVEAADINGY 125
              LQ   +HWAA RGS+     L+ +GA     D  G+
Sbjct: 64  GGDLQAAPIHWAAKRGSVKTVHYLVQHGADPLLKDKQGF 102

BLAST of Carg16304 vs. ExPASy Swiss-Prot
Match: Q6C520 (Palmitoyltransferase AKR1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=AKR1 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.8e-10
Identity = 46/125 (36.80%), Postives = 62/125 (49.60%), Query Frame = 0

Query: 8   VVASPNSKSQTNPEVAIVDVYS-------ASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 67
           V AS   +S TN E A  DV         A+  G+  +V+   E    SVL    +G  A
Sbjct: 28  VTASGKVESTTNAEEATSDVEEEENPLVVAARDGNTAEVKRLCESGSYSVLDTAEDGVTA 87

Query: 68  LQWAALNNFPDIVQYIIEHGADVNS-CDKLQQTALHWAAVRGSIMAADVLLLNGARVEAA 125
           L WAA+NN     QY++E GA V++   +L  T LHWA  RG +     L+ NGA    +
Sbjct: 88  LHWAAVNNRISTCQYLVEQGAVVDAKGGQLNGTPLHWACRRGLVYIVHYLIQNGADPLRS 147

BLAST of Carg16304 vs. ExPASy TrEMBL
Match: A0A6J1IBI1 (S-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111470988 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 7.2e-63
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0

Query: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60
           MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA
Sbjct: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60

Query: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120
           LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD
Sbjct: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120

Query: 121 INGYR 126
           INGYR
Sbjct: 121 INGYR 125

BLAST of Carg16304 vs. ExPASy TrEMBL
Match: A0A6J1GMX6 (S-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455421 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.7e-62
Identity = 124/125 (99.20%), Postives = 124/125 (99.20%), Query Frame = 0

Query: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60
           MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHG SVLVPDSNGYYA
Sbjct: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGPSVLVPDSNGYYA 60

Query: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120
           LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD
Sbjct: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120

Query: 121 INGYR 126
           INGYR
Sbjct: 121 INGYR 125

BLAST of Carg16304 vs. ExPASy TrEMBL
Match: A0A0A0K669 (S-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G067470 PE=3 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.5e-60
Identity = 120/125 (96.00%), Postives = 123/125 (98.40%), Query Frame = 0

Query: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60
           MASSEIEVVASPN +SQTN EVAIVDVYSASAYGDFQKVRSFVE+HGAS+LVPDSNGYYA
Sbjct: 1   MASSEIEVVASPNPRSQTNTEVAIVDVYSASAYGDFQKVRSFVEDHGASLLVPDSNGYYA 60

Query: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120
           LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD
Sbjct: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120

Query: 121 INGYR 126
           INGYR
Sbjct: 121 INGYR 125

BLAST of Carg16304 vs. ExPASy TrEMBL
Match: A0A5D3C6A3 (S-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001740 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 9.7e-60
Identity = 119/125 (95.20%), Postives = 123/125 (98.40%), Query Frame = 0

Query: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60
           MASSEIEVVASPNS+SQTN EVAIVDVYSASAYGDFQKVRSFVE+HGAS+L+PDSNGYYA
Sbjct: 25  MASSEIEVVASPNSRSQTNNEVAIVDVYSASAYGDFQKVRSFVEDHGASLLIPDSNGYYA 84

Query: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120
           LQWAALNNF DIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD
Sbjct: 85  LQWAALNNFSDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 144

Query: 121 INGYR 126
           INGYR
Sbjct: 145 INGYR 149

BLAST of Carg16304 vs. ExPASy TrEMBL
Match: A0A5A7SQ30 (S-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G001750 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 9.7e-60
Identity = 119/125 (95.20%), Postives = 123/125 (98.40%), Query Frame = 0

Query: 1   MASSEIEVVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYA 60
           MASSEIEVVASPNS+SQTN EVAIVDVYSASAYGDFQKVRSFVE+HGAS+L+PDSNGYYA
Sbjct: 25  MASSEIEVVASPNSRSQTNNEVAIVDVYSASAYGDFQKVRSFVEDHGASLLIPDSNGYYA 84

Query: 61  LQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 120
           LQWAALNNF DIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD
Sbjct: 85  LQWAALNNFSDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAAD 144

Query: 121 INGYR 126
           INGYR
Sbjct: 145 INGYR 149

BLAST of Carg16304 vs. TAIR 10
Match: AT2G14255.1 (Ankyrin repeat family protein with DHHC zinc finger domain )

HSP 1 Score: 154.1 bits (388), Expect = 7.9e-38
Identity = 76/126 (60.32%), Postives = 93/126 (73.81%), Query Frame = 0

Query: 1   MASSEIEVV-ASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYY 60
           M SSEIEVV    NS         I DV+SASAYGD  +++ FVE +G+SV +PD NG+Y
Sbjct: 1   MDSSEIEVVPLDSNSHQSPTESPTITDVFSASAYGDLHQLKHFVEHNGSSVSLPDDNGFY 60

Query: 61  ALQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAA 120
           ALQWAALNN   + QYII+HG DVNS D +QQT LHWAAV+GSI  AD+LL +GAR+EA 
Sbjct: 61  ALQWAALNNSLHVAQYIIQHGGDVNSADNIQQTPLHWAAVKGSIDVADLLLQHGARIEAV 120

Query: 121 DINGYR 126
           D+NG+R
Sbjct: 121 DVNGFR 126

BLAST of Carg16304 vs. TAIR 10
Match: AT5G20350.1 (Ankyrin repeat family protein with DHHC zinc finger domain )

HSP 1 Score: 132.5 bits (332), Expect = 2.5e-31
Identity = 70/134 (52.24%), Postives = 89/134 (66.42%), Query Frame = 0

Query: 3   SSEIEVVASPNSKSQTNPEVAIV---------DVYSASAYGDFQKVRSFVEEHGASVLVP 62
           SSEIEVV    S  + N E +           DVY+A+AYGD +K+   VE  G+SV  P
Sbjct: 2   SSEIEVVEEIQSNPKENGESSSKGIEEESLKNDVYTAAAYGDLEKLHRLVECEGSSVSEP 61

Query: 63  DSNGYYALQWAALNNFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNG 122
           D+ GYYALQW+ALNN   + QY+IEHG DVN+ D   QTALHW+AVRG+I  A++LL  G
Sbjct: 62  DALGYYALQWSALNNRVAVAQYLIEHGGDVNATDHTGQTALHWSAVRGAIQVAELLLQEG 121

Query: 123 ARVEAADINGYRVT 128
           ARV+A D+ GY+ T
Sbjct: 122 ARVDATDMYGYQAT 135

BLAST of Carg16304 vs. TAIR 10
Match: AT5G12320.1 (ankyrin repeat family protein )

HSP 1 Score: 59.7 bits (143), Expect = 2.0e-09
Identity = 34/102 (33.33%), Postives = 50/102 (49.02%), Query Frame = 0

Query: 26  DVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYALQWAALNNFPDIVQYIIEHGADVNS 85
           D+  A+ Y D   +R+   + G S+   DS G  AL  AA N    IV+Y+I  G D+N+
Sbjct: 14  DLLEAARYNDIDDLRTLASD-GLSLHSRDSQGRTALHMAAANGHMTIVEYLISEGVDINA 73

Query: 86  CDKLQQTALHWAAVRGSIMAADVLLLNGARVEAADINGYRVT 128
            +      LHWA + G +     L+L GA +    +N Y  T
Sbjct: 74  LNDENNAPLHWACLNGHVEVVKRLILAGASLSL--LNRYERT 112

BLAST of Carg16304 vs. TAIR 10
Match: AT2G03430.1 (Ankyrin repeat family protein )

HSP 1 Score: 54.3 bits (129), Expect = 8.5e-08
Identity = 32/97 (32.99%), Postives = 52/97 (53.61%), Query Frame = 0

Query: 27  VYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYALQWAALNNFPDIVQYIIEHGADVNSC 86
           ++SA++ G+ + V   +   GA V   ++ G  AL +AA     +I Q ++ HGA +N  
Sbjct: 87  LHSAASIGNAELVEVLL-TRGADVNAKNNGGRTALHYAASKGRLEIAQLLLTHGAKINIT 146

Query: 87  DKLQQTALHWAAVRGSIMAADVLLLNGARVEAADING 124
           DK+  T LH AA  G +   + L+  GA ++A D  G
Sbjct: 147 DKVGCTPLHRAASVGKLEVCEFLIEEGAEIDATDKMG 182

BLAST of Carg16304 vs. TAIR 10
Match: AT4G35450.3 (ankyrin repeat-containing protein 2 )

HSP 1 Score: 45.8 bits (107), Expect = 3.0e-05
Identity = 35/115 (30.43%), Postives = 56/115 (48.70%), Query Frame = 0

Query: 8   VVASPNSKSQTNPEVAIVDVYSASAYGDFQKVRSFVEEHGASVLVPDSNGYYALQWAALN 67
           V A P    +   E +I  V+  ++ GD + +++ +   G +    DS G  AL +A   
Sbjct: 205 VSAEPEVAEEGEEEESI--VHQTASLGDVEGLKAALAS-GGNKDEEDSEGRTALHFACGY 264

Query: 68  NFPDIVQYIIEHGADVNSCDKLQQTALHWAAVRGSIMAADVLLLNGARVEAADIN 123
                 Q +I+ GA VN+ DK + T LH+AA  G      +LL NGA V   +++
Sbjct: 265 GELKCAQVLIDAGASVNAVDKNKNTPLHYAAGYGRKECVSLLLENGAAVTLQNLD 316

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7011580.14.2e-65100.00putative protein S-acyltransferase 23 [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_023511407.11.5e-62100.00probable protein S-acyltransferase 23 [Cucurbita pepo subsp. pepo][more]
XP_022972424.11.5e-62100.00probable protein S-acyltransferase 23 [Cucurbita maxima][more]
XP_038888969.12.5e-6298.40probable protein S-acyltransferase 23 isoform X1 [Benincasa hispida] >XP_0388889... [more]
XP_022952854.15.6e-6299.20probable protein S-acyltransferase 23 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q3EC111.1e-3660.32Probable protein S-acyltransferase 23 OS=Arabidopsis thaliana OX=3702 GN=PAT23 P... [more]
Q52T383.5e-3052.24Protein S-acyltransferase 24 OS=Arabidopsis thaliana OX=3702 GN=PAT24 PE=2 SV=1[more]
Q9VUW94.7e-1135.04Palmitoyltransferase Hip14 OS=Drosophila melanogaster OX=7227 GN=Hip14 PE=1 SV=1[more]
Q097018.0e-1135.35Palmitoyltransferase akr1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843)... [more]
Q6C5201.8e-1036.80Palmitoyltransferase AKR1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28... [more]
Match NameE-valueIdentityDescription
A0A6J1IBI17.2e-63100.00S-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111470988 PE=3 SV=1[more]
A0A6J1GMX62.7e-6299.20S-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455421 PE=3 SV=1[more]
A0A0A0K6692.5e-6096.00S-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G067470 PE=3 SV=1[more]
A0A5D3C6A39.7e-6095.20S-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001... [more]
A0A5A7SQ309.7e-6095.20S-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G00... [more]
Match NameE-valueIdentityDescription
AT2G14255.17.9e-3860.32Ankyrin repeat family protein with DHHC zinc finger domain [more]
AT5G20350.12.5e-3152.24Ankyrin repeat family protein with DHHC zinc finger domain [more]
AT5G12320.12.0e-0933.33ankyrin repeat family protein [more]
AT2G03430.18.5e-0832.99Ankyrin repeat family protein [more]
AT4G35450.33.0e-0530.43ankyrin repeat-containing protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 56..85
e-value: 4.2E-5
score: 32.9
coord: 89..118
e-value: 6.5
score: 15.7
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 89..121
score: 9.59106
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 56..88
score: 11.5676
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 4..127
e-value: 6.5E-26
score: 93.2
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILY48403Ankyrin repeatcoord: 28..124
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 30..119
e-value: 3.3E-11
score: 43.6
NoneNo IPR availablePANTHERPTHR24161:SF82PROTEIN S-ACYLTRANSFERASE 24coord: 3..127
NoneNo IPR availablePANTHERPTHR24161FAMILY NOT NAMEDcoord: 3..127
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 56..88
score: 10.205854
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 89..120
score: 8.720098

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16304-RACarg16304-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018230 peptidyl-L-cysteine S-palmitoylation
biological_process GO:0006612 protein targeting to membrane
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding
molecular_function GO:0019706 protein-cysteine S-palmitoyltransferase activity