Carg16294 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16294
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein PAM68, chloroplastic
Location	Carg_Chr06: 3050646 .. 3052307 (-)
RNA-Seq Expression	Carg16294
Synteny	Carg16294

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTCATTACCAAAAACCTTATCCTCTTTCAATGGCCACATCCATGGCGTCCGTCTCTGCTTCTCGCAGTGAAGACGAAATGTAAGAGCCGAGCCGACTGTGTTTCAATTTCAGAGACAAATCCTAATGGCTGCCATTACCGGATCGCTCAACCTTCCTCTTGCGCCTTCAAAGCTTCCATCACTTCACATTACGGTACTCTTCTCACTTCTCCTCGCGATTGCGATTTTCTTCTCGCTACTTCTGAGTTCTGTAGAAAACTTTCCAGCTATAACTACTTTGAATTTTACTGAAACGGACGTAAGATGATGGATTCTTCAATCTGAACTGTTCATCAATCGATTTTTCCTAAGTTTGTTAATCGTCAATAGGTTTCGCTAACATTAGGAATTAGGTTTTAGGATTGATACTTGAAAAAACCTAGAAATCTATTTTGAAACGGAAGGGAAGTGAAGGATTCTTTAATCTGAACTATTCAACACTCGATTTTCCATAAGTTCATAAATCCACAGCCAGTTTCGTAACATTAGGAGTCAGGATTTAAGTTGATACTTGTAAAACCTAGAGCGAATTCATCGATCCATTTCGATTTCAGTTAAGACGAGTGGAGTGATTCAATTACCGATTACGAGACGAATTTCTGTTTGTTCTGCAGAGGAATTATAACTTATCGATCCAATCAAAAAGCCAGAAAAGGAACTATTTCTCACTTTTGAGACCAAGCCTTTACCAAAACCAAACTCGCACGAACCACGCCTTGATTGTAAACGCGACATTGAGAAGTCCAAAAGGATTCGGACCAGCTCCAAGGAAGAAGAAGAAGAAGAAAACAAAAGGAGGAGGAGATGGAGAAGAAGAAAGTGAGGATGAGGAAGATGAGGAAGAAGAGGAGGAGGAGGAAGATCGTGGTGTGATACCAGAAGTAGTGACGAACAGGATGATCAGTAGGATGGGATTTTCAGTGGGGATTCCATTGTTGATAGGACTTTTGTTCTTCCCATTCTTCTATTACCTGAAAGTTGTGCTGAAAATCGATGTGCCCTCATGGGTGCCAGTCATAGTATCCTTCTTCTTCTTTGGTTCTGCTCTCTTGGGAGTGAGCTATGGCATTGTGTCCTCTAGTTGGGATCCCATGAGGGAAGGTTCTCTTTTGGGTTGGAACGAAGCTCAGAAGAATTGGCCTGTTTTTTGGCAGTCCATTTGGAGTGGCGGATCTAATAAGAAATAGATGATTTCTTCTTTTAATGAGAAATATTATATTTTTTCTTCCTATAAATGTTATTTGAGAAGGAAACTTATTACAGTAGTTGAATTAGTTTTTAGTTTTGTTGATTGTTTTAGATTACAAGTTTGGTTCTTCGACTTTTAAGGTTTGGAGAGTCTTAACGGCCGATAGATTTGTGATTTTATGTTTAATAGCTTTTTGAACTATAAAAACTATATAAACTGAAAATTTTATGTTTAAGAGGACTCTCATCTAGCTCACTTTCTTTTAATAAAAGAAAAAAAGCTTAAGGATTTGTTATAGTTCTTAATAGAAACTTTGATCTTTCCATTTTAGGGTTATAAATCTTTAAACCCGTGTGATGTCTCACATTGGTTGGGGAGGAGAACAAACCACCCTTTATAAGGGTGTGGAAACTTTCCCCTAGCAGACGCGT

mRNA sequence

TTTTTCATTACCAAAAACCTTATCCTCTTTCAATGGCCACATCCATGGCGTCCGTCTCTGCTTCTCGCAGTGAAGACGAAATGTAAGAGCCGAGCCGACTGTGTTTCAATTTCAGAGACAAATCCTAATGGCTGCCATTACCGGATCGCTCAACCTTCCTCTTGCGCCTTCAAAGCTTCCATCACTTCACATTACGAGGAATTATAACTTATCGATCCAATCAAAAAGCCAGAAAAGGAACTATTTCTCACTTTTGAGACCAAGCCTTTACCAAAACCAAACTCGCACGAACCACGCCTTGATTGTAAACGCGACATTGAGAAGTCCAAAAGGATTCGGACCAGCTCCAAGGAAGAAGAAGAAGAAGAAAACAAAAGGAGGAGGAGATGGAGAAGAAGAAAGTGAGGATGAGGAAGATGAGGAAGAAGAGGAGGAGGAGGAAGATCGTGGTGTGATACCAGAAGTAGTGACGAACAGGATGATCAGTAGGATGGGATTTTCAGTGGGGATTCCATTGTTGATAGGACTTTTGTTCTTCCCATTCTTCTATTACCTGAAAGTTGTGCTGAAAATCGATGTGCCCTCATGGGTGCCAGTCATAGTATCCTTCTTCTTCTTTGGTTCTGCTCTCTTGGGAGTGAGCTATGGCATTGTGTCCTCTAGTTGGGATCCCATGAGGGAAGGTTCTCTTTTGGGTTGGAACGAAGCTCAGAAGAATTGGCCTGTTTTTTGGCAGTCCATTTGGAGTGGCGGATCTAATAAGAAATAGATGATTTCTTCTTTTAATGAGAAATATTATATTTTTTCTTCCTATAAATGTTATTTGAGAAGGAAACTTATTACAGTAGTTGAATTAGTTTTTAGTTTTGTTGATTGTTTTAGATTACAAGTTTGGTTCTTCGACTTTTAAGGTTTGGAGAGTCTTAACGGCCGATAGATTTGTGATTTTATGTTTAATAGCTTTTTGAACTATAAAAACTATATAAACTGAAAATTTTATGTTTAAGAGGACTCTCATCTAGCTCACTTTCTTTTAATAAAAGAAAAAAAGCTTAAGGATTTGTTATAGTTCTTAATAGAAACTTTGATCTTTCCATTTTAGGGTTATAAATCTTTAAACCCGTGTGATGTCTCACATTGGTTGGGGAGGAGAACAAACCACCCTTTATAAGGGTGTGGAAACTTTCCCCTAGCAGACGCGT

Coding sequence (CDS)

ATGGCTGCCATTACCGGATCGCTCAACCTTCCTCTTGCGCCTTCAAAGCTTCCATCACTTCACATTACGAGGAATTATAACTTATCGATCCAATCAAAAAGCCAGAAAAGGAACTATTTCTCACTTTTGAGACCAAGCCTTTACCAAAACCAAACTCGCACGAACCACGCCTTGATTGTAAACGCGACATTGAGAAGTCCAAAAGGATTCGGACCAGCTCCAAGGAAGAAGAAGAAGAAGAAAACAAAAGGAGGAGGAGATGGAGAAGAAGAAAGTGAGGATGAGGAAGATGAGGAAGAAGAGGAGGAGGAGGAAGATCGTGGTGTGATACCAGAAGTAGTGACGAACAGGATGATCAGTAGGATGGGATTTTCAGTGGGGATTCCATTGTTGATAGGACTTTTGTTCTTCCCATTCTTCTATTACCTGAAAGTTGTGCTGAAAATCGATGTGCCCTCATGGGTGCCAGTCATAGTATCCTTCTTCTTCTTTGGTTCTGCTCTCTTGGGAGTGAGCTATGGCATTGTGTCCTCTAGTTGGGATCCCATGAGGGAAGGTTCTCTTTTGGGTTGGAACGAAGCTCAGAAGAATTGGCCTGTTTTTTGGCAGTCCATTTGGAGTGGCGGATCTAATAAGAAATAG

Protein sequence

MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHALIVNATLRSPKGFGPAPRKKKKKKTKGGGDGEEESEDEEDEEEEEEEEDRGVIPEVVTNRMISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSSWDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK

Homology

[Close]

BLAST of Carg16294 vs. NCBI nr
Match: KAG6596699.1 (Protein PAM68, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028232.1 Protein PAM68, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 407.1 bits (1045), Expect = 9.1e-110
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 0


Query: 1   MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHALIV 60
           MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHALIV
Sbjct: 1   MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHALIV 60

Query: 61  NATLRSPKGFGPAPRKKKKKKTKGGGDGEEESEDEEDEEEEEEEEDRGVIPEVVTNRMIS 120
           NATLRSPKGFGPAPRKKKKKKTKGGGDGEEESEDEEDEEEEEEEEDRGVIPEVVTNRMIS
Sbjct: 61  NATLRSPKGFGPAPRKKKKKKTKGGGDGEEESEDEEDEEEEEEEEDRGVIPEVVTNRMIS 120

Query: 121 RMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSSW 180
           RMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSSW
Sbjct: 121 RMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSSW 180

Query: 181 DPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 214
           DPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK
Sbjct: 181 DPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 213

[Close]

BLAST of Carg16294 vs. NCBI nr
Match: XP_022949106.1 (protein PAM68, chloroplastic [Cucurbita moschata])

HSP 1 Score: 400.2 bits (1027), Expect = 1.1e-107
Identity = 211/213 (99.06%), Postives = 211/213 (99.06%), Query Frame = 0


Query: 1   MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHALIV 60
           MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNH LIV
Sbjct: 1   MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHTLIV 60

Query: 61  NATLRSPKGFGPAPRKKKKKKTKGGGDGEEESEDEEDEEEEEEEEDRGVIPEVVTNRMIS 120
           NATLRSPKGFGPAPRKKKKKKTKGGGDGEEESEDEED EEEEEEEDRGVIPEVVTNRMIS
Sbjct: 61  NATLRSPKGFGPAPRKKKKKKTKGGGDGEEESEDEED-EEEEEEEDRGVIPEVVTNRMIS 120

Query: 121 RMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSSW 180
           RMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSSW
Sbjct: 121 RMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSSW 180

Query: 181 DPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 214
           DPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK
Sbjct: 181 DPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 212

[Close]

BLAST of Carg16294 vs. NCBI nr
Match: XP_023540526.1 (protein PAM68, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 388.7 bits (997), Expect = 3.3e-104
Identity = 207/214 (96.73%), Postives = 208/214 (97.20%), Query Frame = 0


Query: 1   MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHALIV 60
           MAAI GSLNLPLAPS+LPSLHITRNYNLSIQSKS KRNYFSLLRPSLYQNQTRTNH LIV
Sbjct: 1   MAAIAGSLNLPLAPSQLPSLHITRNYNLSIQSKSHKRNYFSLLRPSLYQNQTRTNHTLIV 60

Query: 61  NATLRSPKGFGPAPR-KKKKKKTKGGGDGEEESEDEEDEEEEEEEEDRGVIPEVVTNRMI 120
           NATLRSPKGFGP PR KKKKKKTKGGGDGEEESEDEED EEEEEEEDRGVIPEVVTNRMI
Sbjct: 61  NATLRSPKGFGPTPRKKKKKKKTKGGGDGEEESEDEED-EEEEEEEDRGVIPEVVTNRMI 120

Query: 121 SRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS 180
           SRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS
Sbjct: 121 SRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS 180

Query: 181 WDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 214
           WDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK
Sbjct: 181 WDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 213

[Close]

BLAST of Carg16294 vs. NCBI nr
Match: XP_023005760.1 (protein PAM68, chloroplastic [Cucurbita maxima])

HSP 1 Score: 385.6 bits (989), Expect = 2.8e-103
Identity = 206/215 (95.81%), Postives = 207/215 (96.28%), Query Frame = 0


Query: 1   MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHALIV 60
           MAAITGSLNLPLAPSKLPSLHITR Y LSIQSKS KRNYFSLLRPSLYQNQTRTNH LIV
Sbjct: 1   MAAITGSLNLPLAPSKLPSLHITRKYKLSIQSKSHKRNYFSLLRPSLYQNQTRTNHTLIV 60

Query: 61  NATLRSPKGFGPAPR--KKKKKKTKGGGDGEEESEDEEDEEEEEEEEDRGVIPEVVTNRM 120
           NATLRSPKGFGPAPR  KKKKKKT+GGGDGEEESEDEED  EEEEEEDRGVIPEVVTNRM
Sbjct: 61  NATLRSPKGFGPAPRKKKKKKKKTEGGGDGEEESEDEED--EEEEEEDRGVIPEVVTNRM 120

Query: 121 ISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSS 180
           ISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSS
Sbjct: 121 ISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSS 180

Query: 181 SWDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 214
           SWDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK
Sbjct: 181 SWDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 213

[Close]

BLAST of Carg16294 vs. NCBI nr
Match: XP_008448605.1 (PREDICTED: protein PAM68, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 311.2 bits (796), Expect = 6.8e-81
Identity = 169/217 (77.88%), Postives = 183/217 (84.33%), Query Frame = 0


Query: 1   MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHALIV 60
           MAAI GSLNLPLAPS LP L I +N   SI  KS +RN F   RP++ QNQTRTNH   +
Sbjct: 1   MAAIAGSLNLPLAPSNLPLLRIKKNCEFSIHIKSYERNCFPNFRPTINQNQTRTNHPTSL 60

Query: 61  NATLRSPKGFGPAPRKKKKKKTKGGG----DGEEESEDEEDEEEEEEEEDRGVIPEVVTN 120
           NATLR PKGFGPAPRKKK KKTKG G    D +E+ E++ +EEE+ EEE+ GVIPEVVTN
Sbjct: 61  NATLRRPKGFGPAPRKKKMKKTKGEGREDDDNDEDEEEDNEEEEDNEEEEGGVIPEVVTN 120

Query: 121 RMISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIV 180
           RM+SRMGF+VGIPL IGLLFFPFFYYLKV LKIDVPSWVPVIVSFFFFGSALLGVSYGIV
Sbjct: 121 RMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIV 180

Query: 181 SSSWDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 214
           SSSWDPMREGSLLGWNEAQKNWPVFWQSIW GGSNKK
Sbjct: 181 SSSWDPMREGSLLGWNEAQKNWPVFWQSIW-GGSNKK 216

[Close]

BLAST of Carg16294 vs. ExPASy Swiss-Prot
Match: O49668 (Protein PAM68, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM68 PE=1 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 6.0e-56
Identity = 112/165 (67.88%), Postives = 136/165 (82.42%), Query Frame = 0


Query: 50  NQTRTNHALIVNATLRSPKGFGPAPRK-KKKKKTKGGGDGEEESEDE-EDEEEEEEEEDR 109
           N+   +    ++AT+ SPKGFGP P+K KK KK K G   +E+ +DE ED+++EE+E +R
Sbjct: 50  NRLEISRIAPLHATMNSPKGFGPPPKKTKKSKKPKPGNQSDEDDDDEDEDDDDEEDERER 109

Query: 110 GVIPEVVTNRMISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSA 169
           GVIPE+VTNRMISRMGF+VG+PL IGLLFFPFFYYLKV LK+DVP+WVP IVSF FFG+A
Sbjct: 110 GVIPEIVTNRMISRMGFTVGLPLFIGLLFFPFFYYLKVGLKVDVPTWVPFIVSFVFFGTA 169

Query: 170 LLGVSYGIVSSSWDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNK 213
           L GVSYGIVSSSWDP+REGSLLGWNEA+KNWPVFWQS W+    +
Sbjct: 170 LAGVSYGIVSSSWDPLREGSLLGWNEAKKNWPVFWQSFWNSSDKR 214

[Close]

BLAST of Carg16294 vs. ExPASy Swiss-Prot
Match: Q9LTD9 (Uncharacterized protein PAM68-like OS=Arabidopsis thaliana OX=3702 GN=At5g52780 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.2e-09
Identity = 49/145 (33.79%), Postives = 74/145 (51.03%), Query Frame = 0


Query: 60  VNATLRSPKGFGPAPRKKKKKKTKGGGDGEEESE-DEEDEEEEEEEEDRGVIPEVVTNRM 119
           V     +PK   P  + + + K   G  G + S   E+    + + ED   IP+ V  RM
Sbjct: 21  VKTKSHNPKTLYPNNKPRWESKLHAGPKGFQSSRTSEKPGRPDPDPEDDPPIPQEVFERM 80

Query: 120 ISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSS 179
           + R+  SVG PL +G+        LK     DVP WVP + +   FGS+ LG++YG +S+
Sbjct: 81  MGRIVVSVGTPLGLGVAILKVLEVLKDRNVWDVPLWVPYLTTLVTFGSSALGIAYGSLST 140

Query: 180 SWDPMREGSLLGWNEAQKNWPVFWQ 204
           + DP +  SL G  EA++NW   W+
Sbjct: 141 NLDPAKTNSLFGLKEAKENWVEMWK 165

[Close]

BLAST of Carg16294 vs. ExPASy TrEMBL
Match: A0A6J1GB37 (protein PAM68, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111452558 PE=4 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 5.4e-108
Identity = 211/213 (99.06%), Postives = 211/213 (99.06%), Query Frame = 0


Query: 1   MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHALIV 60
           MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNH LIV
Sbjct: 1   MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHTLIV 60

Query: 61  NATLRSPKGFGPAPRKKKKKKTKGGGDGEEESEDEEDEEEEEEEEDRGVIPEVVTNRMIS 120
           NATLRSPKGFGPAPRKKKKKKTKGGGDGEEESEDEED EEEEEEEDRGVIPEVVTNRMIS
Sbjct: 61  NATLRSPKGFGPAPRKKKKKKTKGGGDGEEESEDEED-EEEEEEEDRGVIPEVVTNRMIS 120

Query: 121 RMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSSW 180
           RMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSSW
Sbjct: 121 RMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSSW 180

Query: 181 DPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 214
           DPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK
Sbjct: 181 DPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 212

[Close]

BLAST of Carg16294 vs. ExPASy TrEMBL
Match: A0A6J1KYA4 (protein PAM68, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111498663 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.4e-103
Identity = 206/215 (95.81%), Postives = 207/215 (96.28%), Query Frame = 0


Query: 1   MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHALIV 60
           MAAITGSLNLPLAPSKLPSLHITR Y LSIQSKS KRNYFSLLRPSLYQNQTRTNH LIV
Sbjct: 1   MAAITGSLNLPLAPSKLPSLHITRKYKLSIQSKSHKRNYFSLLRPSLYQNQTRTNHTLIV 60

Query: 61  NATLRSPKGFGPAPR--KKKKKKTKGGGDGEEESEDEEDEEEEEEEEDRGVIPEVVTNRM 120
           NATLRSPKGFGPAPR  KKKKKKT+GGGDGEEESEDEED  EEEEEEDRGVIPEVVTNRM
Sbjct: 61  NATLRSPKGFGPAPRKKKKKKKKTEGGGDGEEESEDEED--EEEEEEDRGVIPEVVTNRM 120

Query: 121 ISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSS 180
           ISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSS
Sbjct: 121 ISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSS 180

Query: 181 SWDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 214
           SWDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK
Sbjct: 181 SWDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 213

[Close]

BLAST of Carg16294 vs. ExPASy TrEMBL
Match: A0A1S3BKY8 (protein PAM68, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490730 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.3e-81
Identity = 169/217 (77.88%), Postives = 183/217 (84.33%), Query Frame = 0


Query: 1   MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHALIV 60
           MAAI GSLNLPLAPS LP L I +N   SI  KS +RN F   RP++ QNQTRTNH   +
Sbjct: 1   MAAIAGSLNLPLAPSNLPLLRIKKNCEFSIHIKSYERNCFPNFRPTINQNQTRTNHPTSL 60

Query: 61  NATLRSPKGFGPAPRKKKKKKTKGGG----DGEEESEDEEDEEEEEEEEDRGVIPEVVTN 120
           NATLR PKGFGPAPRKKK KKTKG G    D +E+ E++ +EEE+ EEE+ GVIPEVVTN
Sbjct: 61  NATLRRPKGFGPAPRKKKMKKTKGEGREDDDNDEDEEEDNEEEEDNEEEEGGVIPEVVTN 120

Query: 121 RMISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIV 180
           RM+SRMGF+VGIPL IGLLFFPFFYYLKV LKIDVPSWVPVIVSFFFFGSALLGVSYGIV
Sbjct: 121 RMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIV 180

Query: 181 SSSWDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 214
           SSSWDPMREGSLLGWNEAQKNWPVFWQSIW GGSNKK
Sbjct: 181 SSSWDPMREGSLLGWNEAQKNWPVFWQSIW-GGSNKK 216

[Close]

BLAST of Carg16294 vs. ExPASy TrEMBL
Match: A0A1S3BKQ0 (protein PAM68, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490730 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 8.1e-80
Identity = 169/218 (77.52%), Postives = 183/218 (83.94%), Query Frame = 0


Query: 1   MAAITGSLNLPLAPSKLPSLHI-TRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHALI 60
           MAAI GSLNLPLAPS LP L I  +N   SI  KS +RN F   RP++ QNQTRTNH   
Sbjct: 1   MAAIAGSLNLPLAPSNLPLLRIKQKNCEFSIHIKSYERNCFPNFRPTINQNQTRTNHPTS 60

Query: 61  VNATLRSPKGFGPAPRKKKKKKTKGGG----DGEEESEDEEDEEEEEEEEDRGVIPEVVT 120
           +NATLR PKGFGPAPRKKK KKTKG G    D +E+ E++ +EEE+ EEE+ GVIPEVVT
Sbjct: 61  LNATLRRPKGFGPAPRKKKMKKTKGEGREDDDNDEDEEEDNEEEEDNEEEEGGVIPEVVT 120

Query: 121 NRMISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGI 180
           NRM+SRMGF+VGIPL IGLLFFPFFYYLKV LKIDVPSWVPVIVSFFFFGSALLGVSYGI
Sbjct: 121 NRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGI 180

Query: 181 VSSSWDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 214
           VSSSWDPMREGSLLGWNEAQKNWPVFWQSIW GGSNKK
Sbjct: 181 VSSSWDPMREGSLLGWNEAQKNWPVFWQSIW-GGSNKK 217

[Close]

BLAST of Carg16294 vs. ExPASy TrEMBL
Match: A0A6J1CUC6 (protein PAM68, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014847 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 3.1e-79
Identity = 163/212 (76.89%), Postives = 179/212 (84.43%), Query Frame = 0


Query: 1   MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSLYQNQTRTNHALIV 60
           MAAI GSLNLPLAPS LPSL I RN   SI SKS +R  F LLRPS+YQNQTR N   I+
Sbjct: 1   MAAIAGSLNLPLAPSNLPSLRIKRNCKFSIHSKSHERIRFPLLRPSIYQNQTRINQPAIL 60

Query: 61  NATLRSPKGFGPAPRKKKKKKTKGGGDGEEESEDEEDEEEEEEEEDRGVIPEVVTNRMIS 120
            ATLRSPKGFGPAP+K+KKK      + +EE EDE+++E+E+EE + GVIPEVVTNRM+S
Sbjct: 61  RATLRSPKGFGPAPKKRKKKSQ----ESDEEDEDEDEDEDEDEEAEGGVIPEVVTNRMMS 120

Query: 121 RMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSSW 180
           RMGFSVGIPLLIGLLFFPFFYYLKV LKIDVP+WVP IVSFFFFGSALLGVSYGIVSSSW
Sbjct: 121 RMGFSVGIPLLIGLLFFPFFYYLKVGLKIDVPTWVPFIVSFFFFGSALLGVSYGIVSSSW 180

Query: 181 DPMREGSLLGWNEAQKNWPVFWQSIWSGGSNK 213
           DPMREGSLLGWNEAQKNWPVFWQSIW+    K
Sbjct: 181 DPMREGSLLGWNEAQKNWPVFWQSIWAKSDKK 208

[Close]

BLAST of Carg16294 vs. TAIR 10
Match: AT4G19100.1 (Protein of unknown function (DUF3464) )

HSP 1 Score: 218.8 bits (556), Expect = 4.3e-57
Identity = 112/165 (67.88%), Postives = 136/165 (82.42%), Query Frame = 0


Query: 50  NQTRTNHALIVNATLRSPKGFGPAPRK-KKKKKTKGGGDGEEESEDE-EDEEEEEEEEDR 109
           N+   +    ++AT+ SPKGFGP P+K KK KK K G   +E+ +DE ED+++EE+E +R
Sbjct: 50  NRLEISRIAPLHATMNSPKGFGPPPKKTKKSKKPKPGNQSDEDDDDEDEDDDDEEDERER 109

Query: 110 GVIPEVVTNRMISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSA 169
           GVIPE+VTNRMISRMGF+VG+PL IGLLFFPFFYYLKV LK+DVP+WVP IVSF FFG+A
Sbjct: 110 GVIPEIVTNRMISRMGFTVGLPLFIGLLFFPFFYYLKVGLKVDVPTWVPFIVSFVFFGTA 169

Query: 170 LLGVSYGIVSSSWDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNK 213
           L GVSYGIVSSSWDP+REGSLLGWNEA+KNWPVFWQS W+    +
Sbjct: 170 LAGVSYGIVSSSWDPLREGSLLGWNEAKKNWPVFWQSFWNSSDKR 214

[Close]

BLAST of Carg16294 vs. TAIR 10
Match: AT5G52780.1 (Protein of unknown function (DUF3464) )

HSP 1 Score: 63.2 bits (152), Expect = 3.0e-10
Identity = 49/145 (33.79%), Postives = 74/145 (51.03%), Query Frame = 0


Query: 60  VNATLRSPKGFGPAPRKKKKKKTKGGGDGEEESE-DEEDEEEEEEEEDRGVIPEVVTNRM 119
           V     +PK   P  + + + K   G  G + S   E+    + + ED   IP+ V  RM
Sbjct: 21  VKTKSHNPKTLYPNNKPRWESKLHAGPKGFQSSRTSEKPGRPDPDPEDDPPIPQEVFERM 80

Query: 120 ISRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSS 179
           + R+  SVG PL +G+        LK     DVP WVP + +   FGS+ LG++YG +S+
Sbjct: 81  MGRIVVSVGTPLGLGVAILKVLEVLKDRNVWDVPLWVPYLTTLVTFGSSALGIAYGSLST 140

Query: 180 SWDPMREGSLLGWNEAQKNWPVFWQ 204
           + DP +  SL G  EA++NW   W+
Sbjct: 141 NLDPAKTNSLFGLKEAKENWVEMWK 165

The following BLAST results are available for this feature:

BLAST of Carg16294 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6596699.1	9.1e-110	100.00	Protein PAM68, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]
XP_022949106.1	1.1e-107	99.06	protein PAM68, chloroplastic [Cucurbita moschata]	[more]
XP_023540526.1	3.3e-104	96.73	protein PAM68, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_023005760.1	2.8e-103	95.81	protein PAM68, chloroplastic [Cucurbita maxima]	[more]
XP_008448605.1	6.8e-81	77.88	PREDICTED: protein PAM68, chloroplastic isoform X2 [Cucumis melo]	[more]

BLAST of Carg16294 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49668	6.0e-56	67.88	Protein PAM68, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM68 PE=1 SV=1	[more]
Q9LTD9	4.2e-09	33.79	Uncharacterized protein PAM68-like OS=Arabidopsis thaliana OX=3702 GN=At5g52780 ...	[more]

BLAST of Carg16294 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GB37	5.4e-108	99.06	protein PAM68, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111452558 PE=4 ...	[more]
A0A6J1KYA4	1.4e-103	95.81	protein PAM68, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111498663 PE=4 SV...	[more]
A0A1S3BKY8	3.3e-81	77.88	protein PAM68, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490730 ...	[more]
A0A1S3BKQ0	8.1e-80	77.52	protein PAM68, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490730 ...	[more]
A0A6J1CUC6	3.1e-79	76.89	protein PAM68, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014847 PE=4...	[more]

BLAST of Carg16294 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19100.1	4.3e-57	67.88	Protein of unknown function (DUF3464)	[more]
AT5G52780.1	3.0e-10	33.79	Protein of unknown function (DUF3464)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021855	PAM68-like	PFAM	PF11947	DUF3464	coord: 75..204 e-value: 6.9E-51 score: 171.9
IPR021855	PAM68-like	PANTHER	PTHR34575	PROTEIN PAM68, CHLOROPLASTIC	coord: 28..210
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 89..108
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 65..108
None	No IPR available	PANTHER	PTHR34575:SF1	PROTEIN PAM68, CHLOROPLASTIC	coord: 28..210

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16294-RA	Carg16294-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010207	photosystem II assembly
cellular_component	GO:0016021	integral component of membrane