Carg15909 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15909
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionvacuole membrane protein KMS1-like
LocationCarg_Chr08: 7037692 .. 7042401 (+)
RNA-Seq ExpressionCarg15909
SyntenyCarg15909
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGATTTCGATTTCGATTTCGATTTCGATTTCTCTCTTTCGTCAATAACAGATGTTAACTTTCAGATTTTCTTTTTCCATTTTGTCCTCGTAATTTTAGTCATGATTATCGTCGATCTCTGATCTCCTTTTCTCTGTACGGGAGAATAAGATTGAGATTACAAGAATTTCTCTCCCTTTCGATACTGTCTTGTAATTGCAGGTTGCGGAATGGGGACTGGTGAAGAAGCAGCTTCCCTTGCTGCTCATGATTCGGAAGTATCCATTTCAGGCAAGTATTTCCATATCTTCTAATTTTTTTTTATTTTTTTATTTTTTTTGTTCGGCGAAGCATTGATTGATGTAAGAAAGTTGAAGGAAATTTGGATTCTTAATATTGTTTACATTATTGTTATTACTGTTTTATATTTTAAGAGAGATTTGAGTTTGGTGATTGTCTGAAGTTTGTTAATGAAGTGATCCTTCACGGCAATGAGGGGCCTTGATTGATTGAGTGTACTGGTTAATTTCAAACCAACGCAAAAGTGAAATGAAATTCATTTGTGTCTTGCTTCTTCAGTTTGATTTTGTCTGGACGATTTACCGTTTTAGTTGTTGCTTTCACTTGGCTTTCGTGGAATTAATGAGAACAACTATTCTGTCCTATTCAACTTAGCTTAGGGTCGTTCACATTGCATCTTATCTCTTGATGAAATGTCTATAATTTGCAGAATTATGTCGGAAGCACAAACAAGATCTTGACAATTTGACATTGGTTAATCAACCATTCAAAACATTGAAGTTTTTCACTTTGGGTGTTCTTCAATATGGTAAACACTCGATAGCTTACATTTTAGCTAAAGGTGGTTGGCTTTTGCTTTTAAACTTGATTATTGGGGCCGCTGGAATTCTGGTTTTGACAATTGAAGGGCCACATGAAAAGGTATCTCTGCCTTCTTGAATTTATGTTAGCTTCACATCACTCATTTCAGCATTATATGTGTGCATATTTGCATCAAATATTCACTCTTTTCAACAATATGTGTGTATATTTATGCAGCTGCAAGTATTACATCATTTAGGATGAACTGTGATGAATTTTTCATGATCACATTTTGAAGTATGTTTGTGATTTTCGATTTATATATTGGTGTTTTGATATGCTCTATTGATATGGTAGCTTTCTGGATGGATATTCTATATTTTATTAAAATGTTTGTAGGTTTGCCATTATCCAGCAATATATTTTATATGGATACTATCTGTAACTTTAAGCTGTACCATCCTAAATGTGATAGTTCTAAGCCAATGATTGCACTCCTTATGACATTGTTTTATCACGGGTTTCTAAGAATGATGTTCTTTGAAAAAATGTTGTGGATCTAATTCCTGCCTGTTTATGTAGGCTCTTGAGAGTTGTTAAAATTGTACCCAAGAAAATGCTGCAAAAAATTTTATGCACCATATATATACTGAATCAATCCAGTATAAATGCCATATTACAGAAGAGGTTGGAATTTGGAAGTATTTAGAGGAACTGTTAAGATGGTGGATAGAGAAATGATGGAAACTATCCTAGTAAATGTTGTTTGATTGAAGTGGGGATATATCTCTTCATAAATCACCTTGTTGCGCTTTTATATTCAGGAAGTGAATTTATTTCTATCCTTGAAATTGTTGACCTTTTCCTTTTAATTTGGCCATTGCCTTCCAGTTTTCAGGCAATCGTTTTATAAAATAAATCTTCTCTGCTTTTGCAGCATGTGGAGGAGATTGTAAAATACTTCGACTACGGATTGTGGTGGGTGATCCTAGGAGTTGCATCGTCAATTGGACTTGGTAATTAATGTTGTTTGATTCAAGATCCGTGCTAATTATTGTTTCTTCTTTGTAAGACGCAATGTTTTTATCTGATATTTAGATTGCAATTTGCCGTTATCATCAATATTGTATTGATTGTGCTAATCTGTATTGATAGGATGGGATTGAGCATCTGAATCTTATTTGATTTCACGCTCTATCATTTACTTTCAGGGTCTGGCCTGCATACTTTTGTTCTTTATCTGGGGCCTCATATTGGGTTTTTCACGATAAAGGCAGTGCAGTGTGGGCGAGTGGACTTGAAGAGTGCTGTTTATGACACAATACAATTGAAAAGAGGTCCATCATGGCTTGAGAAGGATTGCTCAGAATTTGGACCTCCAGTTTTCACATCACAAGTCCCCCTCAGTAGCATATTGCCCATGGTTCAGATAGAGGCAATTCTCTGGGGTATTGGGACTTCCTTAGGAGAGCTTCCTCCTTACTTCATCTCAAGGGCGGGTATGAGAAATAGTGTGTTTACTAGTATTCGTTAATAGTTATAAGTCAATTAAATTCTTTTGGTGATATGACAATCAGTTGAGCATTTTTTTATATTCCATCTTCTCTTATCCTTTTTTTTTTTCGGTGGGTGGAACTTGTCTATGTAAAAACAGTTTGCAATACTGAAAGAGAGAACATTTCTAGTAGATTTTTCTTTCGTGATCCTAAATAGTAAATAGTCAACATTTTGTAATTTATAGGTAGGAACTACTGGGCAAGGCAATGCTTTGAGGTCATAGTGTAAGTAGTTTTTGCTTAGGGTTGAATGGCTATGGATAATATTGTACTTCTTTTCGAAACCCGTTCTCGACATAATGATGTGAAAATATGATAGAGAAAGATGTAATGGCAAACAAGAGCTGGCTCAAGTTTTTACTTCGGAACTTTGCAATTAGTGCTATGATTATGACCAACAATCTAGTCTGTAATCTGAAATTTTCCAATTGGAAAATGACTTGTTCCAGGTTGTTTATGGCTTTGCCAATTGTAACTTTTTGCATTGCTGATATTCTTTCATATCCAAGCTTCATATTTTATGTTCAAATATATTTCTTCCATTGTGCAGCACGCCTCTCTGGTGGCAGATCAGAAGCAATGGAAGAACTAGATGCCTCTTCAAGGGAAACTGATGGACTCATTTCAACATATTTAAACAGAATCAAGCGATGGTTCCTTTCACATGCTCAACATTTAAATTTCCTTACTATTCTACTGCTTGCTTCGGTTAGTTATGACTTTATTCTCTTCAATTACTGATATTTTATTTTCCTTTTTTTCCTGCCTTCCATTGGGGGTGAGAGTATAACTTTACTGGTTCGCTAATTTCATGTTATTTTTCCCCTTCAAATATGTTATATTCATTACAACTTAGTGGTCATAGGGACTCAATTGTAAATCAGATACATTTTGGTGGCTACCACCTCCCTAGGGTATTAAATACAGCATCAATCATATCATTAGCACGACTGTTTCTGATCTTCATTTCTTATCTTTCAGATTCCAAATCCTCTGTTCGACTTAGCTGGCATCATGTGTGGACAATTTGGAATTCCTTTCTGGGAATTCTTTTTTGCTACGCTGGTTGGAAAAGCTATTATTAAAACTCACATTCAGGTTTGTGAAGTTTTTATATTTTGTTGTCTAATATTATTTGACTTTGTTTGCAAGGAGTTAAATCCCTTTTACATGTTCAAGTTAAAGCCGTGTTTCTTTTGATAATTTGGTGCTTATGATTTCCGTGCAGACCATTTTCATCATTGCTGTTTGCAACAATCAGCTTCTTGATTGGATAGAAAACGAATTATTATGGATACTCAGCTTTGTGCCTGGATTTTCATCTATCTTGCCCGGGCTGGTTGCCAAGCTCAACGCAGTTAAATCTAAGTATCTGAAAGCACCATCTCAAGTCGCGGCTAATGCCAAGGTACTTGCTGATATTGCATTCATCTGAAAATTCTATCAATTTGTTTGCCTGAGAGCTTATCTGCCAAAACCCGTTGTTTACCACATTCATCTTATATGCCTCTTTTCTTCCTCTTTCCCTTCACCTCTCAACATCAGGCCAATAAATGGGACTTTTCGGTTTCCTCCATCTGGAACACTATAGTATGGCTCATGATCATGAACTTCTCTATCAAGATTATGACTTCAACTGCCCAAAGGTACTTGAAGAAACAGCAAGACGAGGAGCTGGCCGCCATGACCGACAAAGTCTCTGCATCAGCATGCTCGAATTAACCCGACCCTAATGCTCTCTCTCCTTTCATCCTTATAACCAAGATTTAGCATAAGTCCCACCGGTTCGAGTAATCGAGAATCTGTCTGGAGATCCTTCTGTTAGAAAGCTGTATAGAATAGGAAGGCATTTTGGAGAAGAGCAAAGTTACAGGCTATACATGATTGGGAAGGCATTTGTTTGGGTAATTATATTAACTTACCCGCTCTTAACCATTGGAAAAATGCACCATTATTCTTTCTTAGTCTCAGACATATATGACTCATTACTTGAATTATGATGGCTTATCACTCATTCTAATATCTCTCTCTTTGGTTGAATAACCTGTGCACATTTTATGGTTGCTGTTGGATTCTCAAAACAGTTAGTCAAGAAATCTCCAATTAAGAAACTTAGTAGTAGTGCCTATGAATTGGGAATCCAGTATTTGATATTTGATAGATAAACCACTAGGTTGGCATCTTTTTCCGACATTATATTTTAAAATCGAAAGACTGTTCCGATGATTCATCTTATCTGGGAATTAGAAATCACGAGGATTATTAGAAATCACGAGCTTCATAGTAGTACAATATTTTTCATGGTAAGTAATAGTAATGGAATTTTTGTGAAGGTGTATTCCTGCCGAGTCTCA

mRNA sequence

CGATTTCGATTTCGATTTCGATTTCGATTTCTCTCTTTCGTCAATAACAGATGTTAACTTTCAGATTTTCTTTTTCCATTTTGTCCTCGTAATTTTAGTCATGATTATCGTCGATCTCTGATCTCCTTTTCTCTGTACGGGAGAATAAGATTGAGATTACAAGAATTTCTCTCCCTTTCGATACTGTCTTGTAATTGCAGGTTGCGGAATGGGGACTGGTGAAGAAGCAGCTTCCCTTGCTGCTCATGATTCGGAAGTATCCATTTCAGAATTATGTCGGAAGCACAAACAAGATCTTGACAATTTGACATTGGTTAATCAACCATTCAAAACATTGAAGTTTTTCACTTTGGGTGTTCTTCAATATGGTAAACACTCGATAGCTTACATTTTAGCTAAAGGTGGTTGGCTTTTGCTTTTAAACTTGATTATTGGGGCCGCTGGAATTCTGGTTTTGACAATTGAAGGGCCACATGAAAAGCATGTGGAGGAGATTGTAAAATACTTCGACTACGGATTGTGGTGGGTGATCCTAGGAGTTGCATCGTCAATTGGACTTGGGTCTGGCCTGCATACTTTTGTTCTTTATCTGGGGCCTCATATTGGGTTTTTCACGATAAAGGCAGTGCAGTGTGGGCGAGTGGACTTGAAGAGTGCTGTTTATGACACAATACAATTGAAAAGAGGTCCATCATGGCTTGAGAAGGATTGCTCAGAATTTGGACCTCCAGTTTTCACATCACAAGTCCCCCTCAGTAGCATATTGCCCATGGTTCAGATAGAGGCAATTCTCTGGGGTATTGGGACTTCCTTAGGAGAGCTTCCTCCTTACTTCATCTCAAGGGCGGCACGCCTCTCTGGTGGCAGATCAGAAGCAATGGAAGAACTAGATGCCTCTTCAAGGGAAACTGATGGACTCATTTCAACATATTTAAACAGAATCAAGCGATGGTTCCTTTCACATGCTCAACATTTAAATTTCCTTACTATTCTACTGCTTGCTTCGATTCCAAATCCTCTGTTCGACTTAGCTGGCATCATGTGTGGACAATTTGGAATTCCTTTCTGGGAATTCTTTTTTGCTACGCTGGTTGGAAAAGCTATTATTAAAACTCACATTCAGACCATTTTCATCATTGCTGTTTGCAACAATCAGCTTCTTGATTGGATAGAAAACGAATTATTATGGATACTCAGCTTTGTGCCTGGATTTTCATCTATCTTGCCCGGGCTGGTTGCCAAGCTCAACGCAGTTAAATCTAAGTATCTGAAAGCACCATCTCAAGTCGCGGCTAATGCCAAGGCCAATAAATGGGACTTTTCGGTTTCCTCCATCTGGAACACTATAGTATGGCTCATGATCATGAACTTCTCTATCAAGATTATGACTTCAACTGCCCAAAGGTACTTGAAGAAACAGCAAGACGAGGAGCTGGCCGCCATGACCGACAAAGTCTCTGCATCAGCATGCTCGAATTAACCCGACCCTAATGCTCTCTCTCCTTTCATCCTTATAACCAAGATTTAGCATAAGTCCCACCGGTTCGAGTAATCGAGAATCTGTCTGGAGATCCTTCTGTTAGAAAGCTGTATAGAATAGGAAGGCATTTTGGAGAAGAGCAAAGTTACAGGCTATACATGATTGGGAAGGCATTTGTTTGGGTAATTATATTAACTTACCCGCTCTTAACCATTGGAAAAATGCACCATTATTCTTTCTTAGTCTCAGACATATATGACTCATTACTTGAATTATGATGGCTTATCACTCATTCTAATATCTCTCTCTTTGGTTGAATAACCTGTGCACATTTTATGGTTGCTGTTGGATTCTCAAAACAGTTAGTCAAGAAATCTCCAATTAAGAAACTTAGTAGTAGTGCCTATGAATTGGGAATCCAGTATTTGATATTTGATAGATAAACCACTAGGTTGGCATCTTTTTCCGACATTATATTTTAAAATCGAAAGACTGTTCCGATGATTCATCTTATCTGGGAATTAGAAATCACGAGGATTATTAGAAATCACGAGCTTCATAGTAGTACAATATTTTTCATGGTAAGTAATAGTAATGGAATTTTTGTGAAGGTGTATTCCTGCCGAGTCTCA

Coding sequence (CDS)

ATGGGGACTGGTGAAGAAGCAGCTTCCCTTGCTGCTCATGATTCGGAAGTATCCATTTCAGAATTATGTCGGAAGCACAAACAAGATCTTGACAATTTGACATTGGTTAATCAACCATTCAAAACATTGAAGTTTTTCACTTTGGGTGTTCTTCAATATGGTAAACACTCGATAGCTTACATTTTAGCTAAAGGTGGTTGGCTTTTGCTTTTAAACTTGATTATTGGGGCCGCTGGAATTCTGGTTTTGACAATTGAAGGGCCACATGAAAAGCATGTGGAGGAGATTGTAAAATACTTCGACTACGGATTGTGGTGGGTGATCCTAGGAGTTGCATCGTCAATTGGACTTGGGTCTGGCCTGCATACTTTTGTTCTTTATCTGGGGCCTCATATTGGGTTTTTCACGATAAAGGCAGTGCAGTGTGGGCGAGTGGACTTGAAGAGTGCTGTTTATGACACAATACAATTGAAAAGAGGTCCATCATGGCTTGAGAAGGATTGCTCAGAATTTGGACCTCCAGTTTTCACATCACAAGTCCCCCTCAGTAGCATATTGCCCATGGTTCAGATAGAGGCAATTCTCTGGGGTATTGGGACTTCCTTAGGAGAGCTTCCTCCTTACTTCATCTCAAGGGCGGCACGCCTCTCTGGTGGCAGATCAGAAGCAATGGAAGAACTAGATGCCTCTTCAAGGGAAACTGATGGACTCATTTCAACATATTTAAACAGAATCAAGCGATGGTTCCTTTCACATGCTCAACATTTAAATTTCCTTACTATTCTACTGCTTGCTTCGATTCCAAATCCTCTGTTCGACTTAGCTGGCATCATGTGTGGACAATTTGGAATTCCTTTCTGGGAATTCTTTTTTGCTACGCTGGTTGGAAAAGCTATTATTAAAACTCACATTCAGACCATTTTCATCATTGCTGTTTGCAACAATCAGCTTCTTGATTGGATAGAAAACGAATTATTATGGATACTCAGCTTTGTGCCTGGATTTTCATCTATCTTGCCCGGGCTGGTTGCCAAGCTCAACGCAGTTAAATCTAAGTATCTGAAAGCACCATCTCAAGTCGCGGCTAATGCCAAGGCCAATAAATGGGACTTTTCGGTTTCCTCCATCTGGAACACTATAGTATGGCTCATGATCATGAACTTCTCTATCAAGATTATGACTTCAACTGCCCAAAGGTACTTGAAGAAACAGCAAGACGAGGAGCTGGCCGCCATGACCGACAAAGTCTCTGCATCAGCATGCTCGAATTAA

Protein sequence

MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAYILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQVPLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLISTYLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQVAANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASACSN
Homology
BLAST of Carg15909 vs. NCBI nr
Match: KAG7026241.1 (Vacuole membrane protein KMS1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 840.9 bits (2171), Expect = 4.9e-240
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 0

Query: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60
           MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240
           PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240

Query: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360
           KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420
           AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA
Sbjct: 361 AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420

Query: 421 CSN 424
           CSN
Sbjct: 421 CSN 423

BLAST of Carg15909 vs. NCBI nr
Match: KAG6593896.1 (Vacuole membrane protein KMS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 838.6 bits (2165), Expect = 2.4e-239
Identity = 422/423 (99.76%), Postives = 422/423 (99.76%), Query Frame = 0

Query: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60
           MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240
           PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240

Query: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360
           KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420
           AANAKA KWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA
Sbjct: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420

Query: 421 CSN 424
           CSN
Sbjct: 421 CSN 423

BLAST of Carg15909 vs. NCBI nr
Match: XP_023514621.1 (vacuole membrane protein KMS1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 838.2 bits (2164), Expect = 3.2e-239
Identity = 421/423 (99.53%), Postives = 422/423 (99.76%), Query Frame = 0

Query: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60
           MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240
           PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240

Query: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFG+PFWEFFFATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGVPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360
           KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420
           AANAKA KWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA
Sbjct: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420

Query: 421 CSN 424
           CSN
Sbjct: 421 CSN 423

BLAST of Carg15909 vs. NCBI nr
Match: XP_022930479.1 (vacuole membrane protein KMS1-like [Cucurbita moschata])

HSP 1 Score: 836.3 bits (2159), Expect = 1.2e-238
Identity = 421/423 (99.53%), Postives = 421/423 (99.53%), Query Frame = 0

Query: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60
           MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDC EFGPPVFTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCLEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240
           PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240

Query: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360
           KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420
           AANAKA KWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA
Sbjct: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420

Query: 421 CSN 424
           CSN
Sbjct: 421 CSN 423

BLAST of Carg15909 vs. NCBI nr
Match: XP_023000445.1 (vacuole membrane protein KMS1-like [Cucurbita maxima])

HSP 1 Score: 830.1 bits (2143), Expect = 8.6e-237
Identity = 418/423 (98.82%), Postives = 420/423 (99.29%), Query Frame = 0

Query: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60
           MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLT+VNQPFKTLKFFTLGVLQYGKHSIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTMVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240
           PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSG RSEAMEELDASSRET+GLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGSRSEAMEELDASSRETNGLIST 240

Query: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360
           KTHIQTIFIIAVCNNQLL WIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV
Sbjct: 301 KTHIQTIFIIAVCNNQLLYWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420
           AANAKA KWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA
Sbjct: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420

Query: 421 CSN 424
           CSN
Sbjct: 421 CSN 423

BLAST of Carg15909 vs. ExPASy Swiss-Prot
Match: Q5XF36 (Vacuole membrane protein KMS1 OS=Arabidopsis thaliana OX=3702 GN=KMS1 PE=1 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 2.4e-165
Identity = 275/411 (66.91%), Postives = 341/411 (82.97%), Query Frame = 0

Query: 7   AASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAYILAKGG 66
           +A +A+  S+V+IS L  KH+++++NLTL  QP  TLK F    +QY K SI+Y+LA GG
Sbjct: 3   SAGVASSSSDVAISALREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGG 62

Query: 67  WLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVL 126
           W +L+  ++  +G L++T++GPH KHVEE+++Y  YGLWW+ LGVASSIGLGSGLHTFVL
Sbjct: 63  WFILITTLLVVSGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTFVL 122

Query: 127 YLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFT----SQVPL 186
           YLGPHI  FT+KA  CGRVDLKSA YDTIQLKR PSWL+K CSEFGPP+      S+VPL
Sbjct: 123 YLGPHIALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPL 182

Query: 187 SSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLISTYL 246
           +SILP VQ+EAILWGIGT+LGELPPYFISRAA +SG   + MEELD SS E  G ++T+L
Sbjct: 183 TSILPQVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHL 242

Query: 247 NRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 306
           NR+KRW L+H+QHLNF T+L+LAS+PNPLFDLAGIMCGQFGIPFWEFF ATL+GKAIIKT
Sbjct: 243 NRVKRWLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKT 302

Query: 307 HIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQVAA 366
           HIQTIFII VCNNQLLDW+ENEL+WILS VPG +S+LPGL AKL+A+K KY+ APS V +
Sbjct: 303 HIQTIFIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPVPS 362

Query: 367 NAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMT 414
           + K  KWDFS +SIWN IVWLM++NF +KI+T+TAQR+LKK+Q++E+A +T
Sbjct: 363 HIKVKKWDFSFASIWNGIVWLMLLNFFVKIVTATAQRHLKKKQEKEMATLT 413

BLAST of Carg15909 vs. ExPASy Swiss-Prot
Match: F4I8Q7 (Vacuole membrane protein KMS2 OS=Arabidopsis thaliana OX=3702 GN=KMS2 PE=1 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 6.6e-155
Identity = 272/420 (64.76%), Postives = 331/420 (78.81%), Query Frame = 0

Query: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60
           MG G  A+S        +IS L  KH+QDL+ LTL +QPFKTL+ F + V  Y +   +Y
Sbjct: 1   MGYGNRASS-----KTPAISGLREKHQQDLEKLTLTSQPFKTLRLFVVAVFLYVRRWSSY 60

Query: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           +LA  GWL+L   I  A   L++T++GPH KHVEE+ +Y  +GLWW+ LGVASSIGLGSG
Sbjct: 61  LLANVGWLILFCSIFVAFAALLVTLDGPHVKHVEELSEYTRFGLWWIFLGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180
           LHTFVLYLGPHI  FTIK +QCGRVDLKSA+YDTIQLKR PSWL+K C EFG PVF+S V
Sbjct: 121 LHTFVLYLGPHIALFTIKVMQCGRVDLKSAIYDTIQLKRSPSWLDKPCHEFGSPVFSSGV 180

Query: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240
           PLSSILP VQIEAILWG+GT+LGELPPYFISRAA LSGG+   M+EL+  S + +G I+ 
Sbjct: 181 PLSSILPQVQIEAILWGLGTALGELPPYFISRAASLSGGK---MKELETCSGDDNGFIAK 240

Query: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
            +N+IK W LSH+Q+LNF TIL+LAS+PNPLFDLAGIMCGQF  PFWEFF ATL+GKAII
Sbjct: 241 RVNQIKSWLLSHSQYLNFFTILILASVPNPLFDLAGIMCGQFEKPFWEFFLATLIGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360
           KTHIQT+FII VCNNQLLDW+ENEL++ILSFVPGF+S LP L AKL  +K KYL A   V
Sbjct: 301 KTHIQTVFIICVCNNQLLDWVENELIYILSFVPGFASALPELTAKLRLMKEKYLIASPPV 360

Query: 361 AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420
           +++    KWD S +S+WN +VWLM++NF  +I+TSTAQRYLKKQQ+EEL A+T+K S ++
Sbjct: 361 SSDINVKKWDLSFASVWNGVVWLMLLNFFGQIVTSTAQRYLKKQQEEELDALTNKSSLTS 412

BLAST of Carg15909 vs. ExPASy Swiss-Prot
Match: Q91ZQ0 (Vacuole membrane protein 1 OS=Rattus norvegicus OX=10116 GN=Vmp1 PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 7.0e-56
Identity = 141/396 (35.61%), Postives = 208/396 (52.53%), Query Frame = 0

Query: 18  SISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAYILAKGGWLLLLNLIIGA 77
           S++E  R+ +++  N+ L  QP  TL++F+L  L   K   + +  +   ++   L++ A
Sbjct: 29  SVNEKKRRDREERQNIVLWRQPLITLQYFSLETLVVLKEWTSKLWHRQSMVVSFFLLL-A 88

Query: 78  AGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
           A +    +EG H+++V+ I K F    +W+ LG+ SS+GLG+GLHTF+LYLGPHI   T+
Sbjct: 89  ALVATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTL 148

Query: 138 KAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQVPLSSILPMVQIEAILWG 197
            A +C  V+     Y    +       E              + L SI+  V+IEA +WG
Sbjct: 149 AAYECNSVNFPEPPYPDQIICPDEEGTE------------GAISLWSIISKVRIEACMWG 208

Query: 198 IGTSLGELPPYFISRAARLSGGR--SEAMEELDASSRETDGLISTYLNRIKRWFLSHAQH 257
           IGT++GELPPYF++RAARLSG     E  +E +      +     + +R K       Q 
Sbjct: 209 IGTAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAE-TAQDFASRAKLAVQKLVQK 268

Query: 258 LNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNN 317
           + F  IL  ASIPNPLFDLAGI CG F +PFW FF ATL+GKAIIK HIQ IF+I   + 
Sbjct: 269 VGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIVTFSK 328

Query: 318 QLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQ------VAANAKANKW 377
            +++            +  F   +PG+   L     +YL+A  Q       A  A+   W
Sbjct: 329 HIVEQ-----------MVAFIGAVPGIGPSLQKPFQEYLEAQRQKLHHRSEAGTAQGENW 388

Query: 378 DFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQ 406
              +S  +  +V  M+  F + I+ S AQ Y K+ Q
Sbjct: 389 ---LSWTFEKLVVAMVCYFILSIINSMAQSYAKRIQ 396

BLAST of Carg15909 vs. ExPASy Swiss-Prot
Match: Q99KU0 (Vacuole membrane protein 1 OS=Mus musculus OX=10090 GN=Vmp1 PE=1 SV=2)

HSP 1 Score: 218.8 bits (556), Expect = 1.2e-55
Identity = 143/397 (36.02%), Postives = 213/397 (53.65%), Query Frame = 0

Query: 18  SISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAYILAKGGWLLLLNLIIGA 77
           S+ E  R+ +++  N+ L  QP  TL++F+L  L   K   + +  +   +++  L++ A
Sbjct: 29  SVHEKKRRDREERQNIVLWRQPLITLQYFSLETLVVLKEWTSKLWHRQS-IVVSFLLLLA 88

Query: 78  AGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
           A +    +EG H+++V+ I K F    +W+ LG+ SS+GLG+GLHTF+LYLGPHI   T+
Sbjct: 89  ALVATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTL 148

Query: 138 KAVQCGRVDLKSAVY-DTIQLKRGPSWLEKDCSEFGPPVFTSQVPLSSILPMVQIEAILW 197
            A +C  V+     Y D I         E++ +E         + L SI+  V+IEA +W
Sbjct: 149 AAYECNSVNFPEPPYPDQIICP------EEEGAE-------GAISLWSIISKVRIEACMW 208

Query: 198 GIGTSLGELPPYFISRAARLSGGR--SEAMEELDASSRETDGLISTYLNRIKRWFLSHAQ 257
           GIGT++GELPPYF++RAARLSG     E  +E +      +     + +R K       Q
Sbjct: 209 GIGTAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAEA-AQDFASRAKLAVQKLVQ 268

Query: 258 HLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCN 317
            + F  IL  ASIPNPLFDLAGI CG F +PFW FF ATL+GKAIIK HIQ IF+I   +
Sbjct: 269 KVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIVTFS 328

Query: 318 NQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQ------VAANAKANK 377
             +++ +             F   +PG+   L     +YL+A  Q       A   +   
Sbjct: 329 KHIVEQMVT-----------FIGAVPGIGPSLQKPFQEYLEAQRQKLHHRSEAGTPQGEN 388

Query: 378 WDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQ 406
           W   +S ++  +V  M+  F + I+ S AQ Y K+ Q
Sbjct: 389 W---LSWMFEKLVVAMVCYFVLSIINSMAQNYAKRIQ 396

BLAST of Carg15909 vs. ExPASy Swiss-Prot
Match: Q5R9K4 (Vacuole membrane protein 1 OS=Pongo abelii OX=9601 GN=VMP1 PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.6e-55
Identity = 141/396 (35.61%), Postives = 212/396 (53.54%), Query Frame = 0

Query: 18  SISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAYILAKGGWLLLLNLIIGA 77
           S++E  R+ +++  N+ L  QP  TL++F+L +L   K   + +  +   +++  L++ A
Sbjct: 29  SVNEKKRREREERQNIVLWRQPLITLQYFSLEILVILKEWTSKLWHRQS-IVVSFLLLLA 88

Query: 78  AGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
             I    +EG H+++V+ I K F    +W+ LG+ SS+GLG+GLHTF+LYLGPHI   T+
Sbjct: 89  VLIATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTL 148

Query: 138 KAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQVPLSSILPMVQIEAILWG 197
            A +C  V+     Y            +  C + G    T  + L SI+  V+IEA +WG
Sbjct: 149 AAYECNSVNFPEPPYPD----------QIICPDEGGTEGT--ISLWSIISKVRIEACMWG 208

Query: 198 IGTSLGELPPYFISRAARLSGGR--SEAMEELDASSRETDGLISTYLNRIKRWFLSHAQH 257
           IGT++GELPPYF++RAARLSG     E  +E +      +     + +R K       Q 
Sbjct: 209 IGTAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAES-AQDFASRAKLAVQKLVQK 268

Query: 258 LNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNN 317
           + F  IL  ASIPNPLFDLAGI CG F +PFW FF ATL+GKAIIK HIQ IF+I   + 
Sbjct: 269 VGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIITFSK 328

Query: 318 QLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQ------VAANAKANKW 377
            +++            +  F   +PG+   L     +YL+A  Q           +   W
Sbjct: 329 HIVEQ-----------MVAFIGAVPGIGPSLQKPFQEYLEAQRQKLHHKSEMGTPQGENW 388

Query: 378 DFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQ 406
              +S ++  +V +M+  F + I+ S AQ Y K+ Q
Sbjct: 389 ---LSWMFEKLVVVMVCYFILSIINSMAQSYAKRIQ 396

BLAST of Carg15909 vs. ExPASy TrEMBL
Match: A0A6J1ERI3 (vacuole membrane protein KMS1-like OS=Cucurbita moschata OX=3662 GN=LOC111436919 PE=4 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 5.8e-239
Identity = 421/423 (99.53%), Postives = 421/423 (99.53%), Query Frame = 0

Query: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60
           MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDC EFGPPVFTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCLEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240
           PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240

Query: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360
           KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420
           AANAKA KWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA
Sbjct: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420

Query: 421 CSN 424
           CSN
Sbjct: 421 CSN 423

BLAST of Carg15909 vs. ExPASy TrEMBL
Match: A0A6J1KIC8 (vacuole membrane protein KMS1-like OS=Cucurbita maxima OX=3661 GN=LOC111494711 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 4.2e-237
Identity = 418/423 (98.82%), Postives = 420/423 (99.29%), Query Frame = 0

Query: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60
           MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLT+VNQPFKTLKFFTLGVLQYGKHSIAY
Sbjct: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTMVNQPFKTLKFFTLGVLQYGKHSIAY 60

Query: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240
           PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSG RSEAMEELDASSRET+GLIST
Sbjct: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGSRSEAMEELDASSRETNGLIST 240

Query: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360
           KTHIQTIFIIAVCNNQLL WIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV
Sbjct: 301 KTHIQTIFIIAVCNNQLLYWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360

Query: 361 AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420
           AANAKA KWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA
Sbjct: 361 AANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420

Query: 421 CSN 424
           CSN
Sbjct: 421 CSN 423

BLAST of Carg15909 vs. ExPASy TrEMBL
Match: A0A0A0KED1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G424010 PE=4 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 3.6e-220
Identity = 383/424 (90.33%), Postives = 406/424 (95.75%), Query Frame = 0

Query: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60
           MGT EEAAS+A+HDSEVSISEL +K KQ+L+NLTLVNQPFKTLKFF LGVLQY K SIAY
Sbjct: 1   MGTVEEAASIASHDSEVSISELRKKQKQELENLTLVNQPFKTLKFFILGVLQYSKRSIAY 60

Query: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWLLLLNLIIGAAGIL+LTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGPSWLEK+CSEFGPP+FTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKECSEFGPPLFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240
           PLSSILPMVQ+EAILWGIGT+LGELPPYFISRAARLSG RSEAMEELDASSRE++G I T
Sbjct: 181 PLSSILPMVQVEAILWGIGTALGELPPYFISRAARLSGARSEAMEELDASSRESNGFIPT 240

Query: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNR+KRWFLSHAQHLNFLTILLLAS+PNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQ- 360
           KTHIQTIFIIAVCNNQLLDWIENEL+WILSFVPGFSS+LPGL+AKLNA+K+KYLKAPSQ 
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPGLIAKLNAIKAKYLKAPSQA 360

Query: 361 VAANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSAS 420
           +  N K  KWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQD+ELAA+T+KV AS
Sbjct: 361 MTTNPKVKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKVPAS 420

Query: 421 ACSN 424
            CSN
Sbjct: 421 TCSN 424

BLAST of Carg15909 vs. ExPASy TrEMBL
Match: A0A6J1C9E2 (vacuole membrane protein KMS1-like OS=Momordica charantia OX=3673 GN=LOC111009594 PE=4 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.5e-218
Identity = 380/423 (89.83%), Postives = 403/423 (95.27%), Query Frame = 0

Query: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60
           MGT EEAAS+A+ DSEVS+SEL  +HKQ+LDNLTLV QPFKTLKFF LGVLQY K SIAY
Sbjct: 1   MGTCEEAASIASRDSEVSLSELRTRHKQELDNLTLVTQPFKTLKFFLLGVLQYSKSSIAY 60

Query: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           IL+KGGWLLLLN+IIGAAGIL+L IEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILSKGGWLLLLNVIIGAAGILILNIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGPSWLEKDCSEFGPPVFTS+V
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAPYDTIQLKRGPSWLEKDCSEFGPPVFTSRV 180

Query: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240
           PLSSILPMVQ+EAILWGIGT+LGELPPYFISRAARLSGGRSEAMEELDASSRET+G I  
Sbjct: 181 PLSSILPMVQLEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPK 240

Query: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           +LNRIKRWFLSHAQHLNF+TILLLAS+PNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 HLNRIKRWFLSHAQHLNFVTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360
           KTHIQTIFIIAVCNNQLLDWIENEL+W+LSFVPGFSSILPGL+AKLN+VK+KYLKAPS  
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELIWVLSFVPGFSSILPGLIAKLNSVKAKYLKAPSHA 360

Query: 361 AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420
           AAN K  KWDFSVSS WNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAA+T+KV ASA
Sbjct: 361 AANTKDKKWDFSVSSXWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAALTNKVPASA 420

Query: 421 CSN 424
           C+N
Sbjct: 421 CTN 423

BLAST of Carg15909 vs. ExPASy TrEMBL
Match: A0A6J1H384 (vacuole membrane protein KMS1-like OS=Cucurbita moschata OX=3662 GN=LOC111460087 PE=4 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 3.3e-218
Identity = 381/423 (90.07%), Postives = 403/423 (95.27%), Query Frame = 0

Query: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60
           MGT EEAAS+A+HDSEVSISEL RK K++L+NLTLV QPFKTLKFF LGVLQY K SIAY
Sbjct: 1   MGTVEEAASIASHDSEVSISELSRKQKEELENLTLVTQPFKTLKFFILGVLQYSKRSIAY 60

Query: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGW LLL+LIIG AGIL+LTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGPSWLEKDCSEFGP VFTSQV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240
           PLSSILPMVQIEAILWGIGT+LGELPPYFISRAARLSGGRSEAMEEL+ASSRET+G I T
Sbjct: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELNASSRETNGFIPT 240

Query: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNR+KRWFLSHAQHLNFLTILLLAS+PNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360
           KTHIQTIFIIAVCNNQLLDWIENEL+WILSFVPGFSS+LP L++KLNA+K+KYLKAPS  
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360

Query: 361 AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420
           A N KA KWDFSVSSIWNTIVWLMIMNFS+KIMTSTAQRYLK+QQDEELAA+T+KV ASA
Sbjct: 361 ATNVKA-KWDFSVSSIWNTIVWLMIMNFSVKIMTSTAQRYLKQQQDEELAALTNKVPASA 420

Query: 421 CSN 424
           CSN
Sbjct: 421 CSN 422

BLAST of Carg15909 vs. TAIR 10
Match: AT4G14950.1 (SNARE associated Golgi protein family )

HSP 1 Score: 583.2 bits (1502), Expect = 1.7e-166
Identity = 275/411 (66.91%), Postives = 341/411 (82.97%), Query Frame = 0

Query: 7   AASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAYILAKGG 66
           +A +A+  S+V+IS L  KH+++++NLTL  QP  TLK F    +QY K SI+Y+LA GG
Sbjct: 3   SAGVASSSSDVAISALREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGG 62

Query: 67  WLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVL 126
           W +L+  ++  +G L++T++GPH KHVEE+++Y  YGLWW+ LGVASSIGLGSGLHTFVL
Sbjct: 63  WFILITTLLVVSGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTFVL 122

Query: 127 YLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFT----SQVPL 186
           YLGPHI  FT+KA  CGRVDLKSA YDTIQLKR PSWL+K CSEFGPP+      S+VPL
Sbjct: 123 YLGPHIALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPL 182

Query: 187 SSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLISTYL 246
           +SILP VQ+EAILWGIGT+LGELPPYFISRAA +SG   + MEELD SS E  G ++T+L
Sbjct: 183 TSILPQVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHL 242

Query: 247 NRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 306
           NR+KRW L+H+QHLNF T+L+LAS+PNPLFDLAGIMCGQFGIPFWEFF ATL+GKAIIKT
Sbjct: 243 NRVKRWLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKT 302

Query: 307 HIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQVAA 366
           HIQTIFII VCNNQLLDW+ENEL+WILS VPG +S+LPGL AKL+A+K KY+ APS V +
Sbjct: 303 HIQTIFIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPVPS 362

Query: 367 NAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMT 414
           + K  KWDFS +SIWN IVWLM++NF +KI+T+TAQR+LKK+Q++E+A +T
Sbjct: 363 HIKVKKWDFSFASIWNGIVWLMLLNFFVKIVTATAQRHLKKKQEKEMATLT 413

BLAST of Carg15909 vs. TAIR 10
Match: AT4G14950.3 (SNARE associated Golgi protein family )

HSP 1 Score: 573.5 bits (1477), Expect = 1.4e-163
Identity = 270/400 (67.50%), Postives = 332/400 (83.00%), Query Frame = 0

Query: 18  SISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAYILAKGGWLLLLNLIIGA 77
           S + L  KH+++++NLTL  QP  TLK F    +QY K SI+Y+LA GGW +L+  ++  
Sbjct: 4   SQATLREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGGWFILITTLLVV 63

Query: 78  AGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
           +G L++T++GPH KHVEE+++Y  YGLWW+ LGVASSIGLGSGLHTFVLYLGPHI  FT+
Sbjct: 64  SGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTFVLYLGPHIALFTL 123

Query: 138 KAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFT----SQVPLSSILPMVQIEA 197
           KA  CGRVDLKSA YDTIQLKR PSWL+K CSEFGPP+      S+VPL+SILP VQ+EA
Sbjct: 124 KATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPLTSILPQVQLEA 183

Query: 198 ILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLISTYLNRIKRWFLSHA 257
           ILWGIGT+LGELPPYFISRAA +SG   + MEELD SS E  G ++T+LNR+KRW L+H+
Sbjct: 184 ILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHLNRVKRWLLTHS 243

Query: 258 QHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVC 317
           QHLNF T+L+LAS+PNPLFDLAGIMCGQFGIPFWEFF ATL+GKAIIKTHIQTIFII VC
Sbjct: 244 QHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKTHIQTIFIICVC 303

Query: 318 NNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQVAANAKANKWDFSV 377
           NNQLLDW+ENEL+WILS VPG +S+LPGL AKL+A+K KY+ APS V ++ K  KWDFS 
Sbjct: 304 NNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPVPSHIKVKKWDFSF 363

Query: 378 SSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMT 414
           +SIWN IVWLM++NF +KI+T+TAQR+LKK+Q++E+A +T
Sbjct: 364 ASIWNGIVWLMLLNFFVKIVTATAQRHLKKKQEKEMATLT 403

BLAST of Carg15909 vs. TAIR 10
Match: AT1G05360.1 (BEST Arabidopsis thaliana protein match is: SNARE associated Golgi protein family (TAIR:AT4G14950.1); Has 332 Blast hits to 326 proteins in 115 species: Archae - 0; Bacteria - 11; Metazoa - 174; Fungi - 0; Plants - 74; Viruses - 0; Other Eukaryotes - 73 (source: NCBI BLink). )

HSP 1 Score: 548.5 bits (1412), Expect = 4.7e-156
Identity = 272/420 (64.76%), Postives = 331/420 (78.81%), Query Frame = 0

Query: 1   MGTGEEAASLAAHDSEVSISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAY 60
           MG G  A+S        +IS L  KH+QDL+ LTL +QPFKTL+ F + V  Y +   +Y
Sbjct: 1   MGYGNRASS-----KTPAISGLREKHQQDLEKLTLTSQPFKTLRLFVVAVFLYVRRWSSY 60

Query: 61  ILAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           +LA  GWL+L   I  A   L++T++GPH KHVEE+ +Y  +GLWW+ LGVASSIGLGSG
Sbjct: 61  LLANVGWLILFCSIFVAFAALLVTLDGPHVKHVEELSEYTRFGLWWIFLGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQV 180
           LHTFVLYLGPHI  FTIK +QCGRVDLKSA+YDTIQLKR PSWL+K C EFG PVF+S V
Sbjct: 121 LHTFVLYLGPHIALFTIKVMQCGRVDLKSAIYDTIQLKRSPSWLDKPCHEFGSPVFSSGV 180

Query: 181 PLSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLIST 240
           PLSSILP VQIEAILWG+GT+LGELPPYFISRAA LSGG+   M+EL+  S + +G I+ 
Sbjct: 181 PLSSILPQVQIEAILWGLGTALGELPPYFISRAASLSGGK---MKELETCSGDDNGFIAK 240

Query: 241 YLNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
            +N+IK W LSH+Q+LNF TIL+LAS+PNPLFDLAGIMCGQF  PFWEFF ATL+GKAII
Sbjct: 241 RVNQIKSWLLSHSQYLNFFTILILASVPNPLFDLAGIMCGQFEKPFWEFFLATLIGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQV 360
           KTHIQT+FII VCNNQLLDW+ENEL++ILSFVPGF+S LP L AKL  +K KYL A   V
Sbjct: 301 KTHIQTVFIICVCNNQLLDWVENELIYILSFVPGFASALPELTAKLRLMKEKYLIASPPV 360

Query: 361 AANAKANKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASA 420
           +++    KWD S +S+WN +VWLM++NF  +I+TSTAQRYLKKQQ+EEL A+T+K S ++
Sbjct: 361 SSDINVKKWDLSFASVWNGVVWLMLLNFFGQIVTSTAQRYLKKQQEEELDALTNKSSLTS 412

BLAST of Carg15909 vs. TAIR 10
Match: AT4G14950.2 (SNARE associated Golgi protein family )

HSP 1 Score: 508.1 bits (1307), Expect = 7.0e-144
Identity = 240/352 (68.18%), Postives = 290/352 (82.39%), Query Frame = 0

Query: 18  SISELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAYILAKGGWLLLLNLIIGA 77
           S + L  KH+++++NLTL  QP  TLK F    +QY K SI+Y+LA GGW +L+  ++  
Sbjct: 4   SQATLREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGGWFILITTLLVV 63

Query: 78  AGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
           +G L++T++GPH KHVEE+++Y  YGLWW+ LGVASSIGLGSGLHTFVLYLGPHI  FT+
Sbjct: 64  SGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTFVLYLGPHIALFTL 123

Query: 138 KAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFT----SQVPLSSILPMVQIEA 197
           KA  CGRVDLKSA YDTIQLKR PSWL+K CSEFGPP+      S+VPL+SILP VQ+EA
Sbjct: 124 KATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPLTSILPQVQLEA 183

Query: 198 ILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLISTYLNRIKRWFLSHA 257
           ILWGIGT+LGELPPYFISRAA +SG   + MEELD SS E  G ++T+LNR+KRW L+H+
Sbjct: 184 ILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHLNRVKRWLLTHS 243

Query: 258 QHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVC 317
           QHLNF T+L+LAS+PNPLFDLAGIMCGQFGIPFWEFF ATL+GKAIIKTHIQTIFII VC
Sbjct: 244 QHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKTHIQTIFIICVC 303

Query: 318 NNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQVAANAK 366
           NNQLLDW+ENEL+WILS VPG +S+LPGL AKL+A+K KY+ APS V ++ K
Sbjct: 304 NNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPVPSHIK 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7026241.14.9e-240100.00Vacuole membrane protein KMS1 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6593896.12.4e-23999.76Vacuole membrane protein KMS1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023514621.13.2e-23999.53vacuole membrane protein KMS1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022930479.11.2e-23899.53vacuole membrane protein KMS1-like [Cucurbita moschata][more]
XP_023000445.18.6e-23798.82vacuole membrane protein KMS1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q5XF362.4e-16566.91Vacuole membrane protein KMS1 OS=Arabidopsis thaliana OX=3702 GN=KMS1 PE=1 SV=1[more]
F4I8Q76.6e-15564.76Vacuole membrane protein KMS2 OS=Arabidopsis thaliana OX=3702 GN=KMS2 PE=1 SV=1[more]
Q91ZQ07.0e-5635.61Vacuole membrane protein 1 OS=Rattus norvegicus OX=10116 GN=Vmp1 PE=1 SV=1[more]
Q99KU01.2e-5536.02Vacuole membrane protein 1 OS=Mus musculus OX=10090 GN=Vmp1 PE=1 SV=2[more]
Q5R9K41.6e-5535.61Vacuole membrane protein 1 OS=Pongo abelii OX=9601 GN=VMP1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1ERI35.8e-23999.53vacuole membrane protein KMS1-like OS=Cucurbita moschata OX=3662 GN=LOC111436919... [more]
A0A6J1KIC84.2e-23798.82vacuole membrane protein KMS1-like OS=Cucurbita maxima OX=3661 GN=LOC111494711 P... [more]
A0A0A0KED13.6e-22090.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G424010 PE=4 SV=1[more]
A0A6J1C9E21.5e-21889.83vacuole membrane protein KMS1-like OS=Momordica charantia OX=3673 GN=LOC11100959... [more]
A0A6J1H3843.3e-21890.07vacuole membrane protein KMS1-like OS=Cucurbita moschata OX=3662 GN=LOC111460087... [more]
Match NameE-valueIdentityDescription
AT4G14950.11.7e-16666.91SNARE associated Golgi protein family [more]
AT4G14950.31.4e-16367.50SNARE associated Golgi protein family [more]
AT1G05360.14.7e-15664.76BEST Arabidopsis thaliana protein match is: SNARE associated Golgi protein famil... [more]
AT4G14950.27.0e-14468.18SNARE associated Golgi protein family [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032816SNARE associated Golgi proteinPFAMPF09335SNARE_assoccoord: 198..299
e-value: 6.2E-9
score: 36.3
NoneNo IPR availablePANTHERPTHR10281MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATEDcoord: 1..422
NoneNo IPR availablePANTHERPTHR10281:SF82VACUOLE MEMBRANE PROTEIN KMS1-LIKEcoord: 1..422

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15909-RACarg15909-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane