Carg15717 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg15717
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like
Location	Carg_Chr19: 8751970 .. 8752964 (-)
RNA-Seq Expression	Carg15717
Synteny	Carg15717

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAATAATTTTGCGCTCTTTCTTCTCATTCTTCTGATTCTCGGCAGCAGCGCGGCGGAGTCCCGGCAGCCGTCCACCAGGGACCAAAGCGGCGCGACGGAGCTCTGGTGCGTGGCCAAGAACAACGCCGACGACGCTTCTCTGCAATCCGCCCTCGACTGGGCCTGCGGCGCCGGCGGTGCCGACTGTTCACCAATCCAGCCCGGTGGCGCATGCTACGACGCATCCAATGTCCAGAATATGGCCTCCTTCGCCTTCAACGACTACTTCCGTAAGCACGGCATGACCGACGACAGTTGCTTCTTCCAAAACGCCGCGGCTATCACCTCCTTAAACCCTAGTCTGTTTCTTCACTCCGATCTCTCAGTTTCTTAGCCGAATCTGTTCTTTAATTCAAAACCTTTCCAGATCTGTAACTGAAATGCTATTTGAATTTGTGTTCTGATTTTGATCTCTCTTGTTTTCAGGCTACGGAAATTGCAGAATCCCAGCTAGGTAAATTTCATTGTTGATCTTTAAAACCTAGTAATCAAGAAAAATAATGTGGATCATGGTTGAAATCATCGGCCACCAATGATTCTGCAATTATTTTTCTTCTTTTTCTAGTTTTAATTGGATTTGATTTCTCATTTTATGGCGCAGTTCGTTGAGAAATGGGAACTTTTCAAGCCAGACACCATCGGTAGGGTTGGGGCCGAGTGAAGACATAAGCGGGTCGGGTCGGAAATCGGGCCGGTCACGGGTTTGGCCTATGGTAATCGGGTATTTGTTTTTTAGAATTGTGTTTCAATCTCAAATAGGAAGCTCTAGTTTTTGTATTTGAATTTTGATTTTTTATTAAGCAAATATTGGTTATTAGATGTCTCAAATTCTTATTTTATATGATTTTTTTAATGACTTGGGGCAAAAGAAAAAGATTTTCTGTATAAAGGGTTTATCAAAAGCTAAAATATAATTTTATAAATAATTTCATAATAAATAAAAATTAAGGT

mRNA sequence

ATGGCCAATAATTTTGCGCTCTTTCTTCTCATTCTTCTGATTCTCGGCAGCAGCGCGGCGGAGTCCCGGCAGCCGTCCACCAGGGACCAAAGCGGCGCGACGGAGCTCTGGTGCGTGGCCAAGAACAACGCCGACGACGCTTCTCTGCAATCCGCCCTCGACTGGGCCTGCGGCGCCGGCGGTGCCGACTGTTCACCAATCCAGCCCGGTGGCGCATGCTACGACGCATCCAATGTCCAGAATATGGCCTCCTTCGCCTTCAACGACTACTTCCGCTACGGAAATTGCAGAATCCCAGCTAGTTCGTTGAGAAATGGGAACTTTTCAAGCCAGACACCATCGGTAGGGTTGGGGCCGAGTGAAGACATAAGCGGGTCGGGTCGGAAATCGGGCCGGTCACGGGTTTGGCCTATGGTAATCGGGTATTTGTTTTTTAGAATTGTGTTTCAATCTCAAATAGGAAGCTCTAGTTTTTGTATTTGAATTTTGATTTTTTATTAAGCAAATATTGGTTATTAGATGTCTCAAATTCTTATTTTATATGATTTTTTTAATGACTTGGGGCAAAAGAAAAAGATTTTCTGTATAAAGGGTTTATCAAAAGCTAAAATATAATTTTATAAATAATTTCATAATAAATAAAAATTAAGGT

Coding sequence (CDS)

ATGGCCAATAATTTTGCGCTCTTTCTTCTCATTCTTCTGATTCTCGGCAGCAGCGCGGCGGAGTCCCGGCAGCCGTCCACCAGGGACCAAAGCGGCGCGACGGAGCTCTGGTGCGTGGCCAAGAACAACGCCGACGACGCTTCTCTGCAATCCGCCCTCGACTGGGCCTGCGGCGCCGGCGGTGCCGACTGTTCACCAATCCAGCCCGGTGGCGCATGCTACGACGCATCCAATGTCCAGAATATGGCCTCCTTCGCCTTCAACGACTACTTCCGCTACGGAAATTGCAGAATCCCAGCTAGTTCGTTGAGAAATGGGAACTTTTCAAGCCAGACACCATCGGTAGGGTTGGGGCCGAGTGAAGACATAAGCGGGTCGGGTCGGAAATCGGGCCGGTCACGGGTTTGGCCTATGGTAATCGGGTATTTGTTTTTTAGAATTGTGTTTCAATCTCAAATAGGAAGCTCTAGTTTTTGTATTTGA

Protein sequence

MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYFRYGNCRIPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSRVWPMVIGYLFFRIVFQSQIGSSSFCI

Homology

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BLAST of Carg15717 vs. NCBI nr
Match: KAG7012010.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 322.0 bits (824), Expect = 2.9e-84
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRYGNCRIPASSLRNGNFSSQTPSVGLGPS 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFRYGNCRIPASSLRNGNFSSQTPSVGLGPS
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRYGNCRIPASSLRNGNFSSQTPSVGLGPS 120

Query: 121 EDISGSGRKSGRSRVWPMVIGYLFFRIVFQSQIGSSSFCI 161
           EDISGSGRKSGRSRVWPMVIGYLFFRIVFQSQIGSSSFCI
Sbjct: 121 EDISGSGRKSGRSRVWPMVIGYLFFRIVFQSQIGSSSFCI 160

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BLAST of Carg15717 vs. NCBI nr
Match: XP_022952645.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucurbita moschata])

HSP 1 Score: 305.8 bits (782), Expect = 2.1e-79
Identity = 159/183 (86.89%), Postives = 159/183 (86.89%), Query Frame = 0


Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFR-----------------------YGNCR 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFR                       YGNCR
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSRVWPMVIGYLFFRIVFQSQIGSSS 161
           IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRS VWPMVIGYLFFRIVFQSQIGSSS
Sbjct: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS 180

[Close]

BLAST of Carg15717 vs. NCBI nr
Match: XP_023554742.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 305.4 bits (781), Expect = 2.8e-79
Identity = 158/183 (86.34%), Postives = 159/183 (86.89%), Query Frame = 0


Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFR-----------------------YGNCR 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFR                       YGNCR
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSRVWPMVIGYLFFRIVFQSQIGSSS 161
           IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRS VWPMVIGYLFFRIVFQSQIGSSS
Sbjct: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS 180

[Close]

BLAST of Carg15717 vs. NCBI nr
Match: XP_022969385.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucurbita maxima])

HSP 1 Score: 292.7 bits (748), Expect = 1.9e-75
Identity = 153/183 (83.61%), Postives = 156/183 (85.25%), Query Frame = 0


Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTR+QSGAT+LWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRNQSGATDLWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFR-----------------------YGNCR 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFR                       YG+CR
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGSCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSRVWPMVIGYLFFRIVFQSQIGSSS 161
           IPASSLRNGNF SQTPSVGLGPSEDISGSGRKSGRS VWPMVIGYLFFRIVFQSQIGS S
Sbjct: 121 IPASSLRNGNFPSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSPS 180

[Close]

BLAST of Carg15717 vs. NCBI nr
Match: XP_023554741.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 264.2 bits (674), Expect = 7.1e-67
Identity = 137/162 (84.57%), Postives = 138/162 (85.19%), Query Frame = 0


Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFR-----------------------YGNCR 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFR                       YGNCR
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSRVWPMV 140
           IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRS VWPM+
Sbjct: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMI 162

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BLAST of Carg15717 vs. ExPASy Swiss-Prot
Match: Q9M2K6 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5 PE=2 SV=2)

HSP 1 Score: 97.4 bits (241), Expect = 1.5e-19
Identity = 60/136 (44.12%), Postives = 77/136 (56.62%), Query Frame = 0


Query: 10  LILLILGSSAAESRQPSTRDQSG---ATELWCVAKNNADDASLQSALDWACGAGGADCSP 69
           L L IL ++AA     +T    G     ELWCVAKNNA+D+SLQ+A++WACG GGADC P
Sbjct: 11  LRLSILAATAAMLMVITTAQIGGQVIQVELWCVAKNNAEDSSLQTAIEWACGQGGADCGP 70

Query: 70  IQPGGACYDASNVQNMASFAFNDYFRYGNCRIPASSLRNG------NFSSQTPSVGLGPS 129
           IQ GG C D ++VQ MASF FN+Y+           L+NG      NF++      L PS
Sbjct: 71  IQQGGPCNDPTDVQKMASFVFNNYY-----------LKNGEEDEACNFNNNAALTSLNPS 130

Query: 130 EDI----SGSGRKSGR 133
           +      S  G  +GR
Sbjct: 131 QGTCKYPSSKGANNGR 135

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BLAST of Carg15717 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 71.2 bits (173), Expect = 1.2e-11
Identity = 30/58 (51.72%), Postives = 43/58 (74.14%), Query Frame = 0


Query: 35  ELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYFR 93
           + +C+A +  D  +LQ+ALDWACG G ++CS IQPG +CY  +NV+  ASFAFN Y++
Sbjct: 379 QTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQ 436

[Close]

BLAST of Carg15717 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.4e-11
Identity = 41/119 (34.45%), Postives = 55/119 (46.22%), Query Frame = 0


Query: 16  GSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYD 75
           G S       S  + SG++  WC+A + A +  L+ ALDWACG G  DC+ IQP   C+ 
Sbjct: 348 GKSNGFHSNSSGTNSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQ 407

Query: 76  ASNVQNMASFAFNDYFRYGNCRIPASSLRNGNFSSQTPSVGLGPSED----ISGSGRKS 131
              + + ASF FN YF+       A S     F      V   PS D    I+  G K+
Sbjct: 408 PDTLVSHASFVFNSYFQQNRATDVACS-----FGGAGVKVNKDPSYDKCIYITAGGNKT 461

[Close]

BLAST of Carg15717 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.4e-11
Identity = 31/59 (52.54%), Postives = 44/59 (74.58%), Query Frame = 0


Query: 34  TELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYFR 93
           + ++CVAK +ADD  L   L+WACG G A+C+ IQPG  CY  ++V++ ASFAFNDY++
Sbjct: 359 SSMFCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQ 417

[Close]

BLAST of Carg15717 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 9.8e-11
Identity = 36/89 (40.45%), Postives = 52/89 (58.43%), Query Frame = 0


Query: 32  GATELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYF 91
           G T+ WC+A + A    LQ+ALDWACG G  DCS +QP   C++   V + AS+AFN Y+
Sbjct: 386 GGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYY 445

Query: 92  RYGNCRIPASSLRNGNFSSQTPSVGLGPS 121
           +       ASS+ + +F+  +  V   PS
Sbjct: 446 QQSG----ASSI-DCSFNGASVEVDKDPS 469

[Close]

BLAST of Carg15717 vs. ExPASy TrEMBL
Match: A0A6J1GMC7 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita moschata OX=3662 GN=LOC111455276 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.0e-79
Identity = 159/183 (86.89%), Postives = 159/183 (86.89%), Query Frame = 0


Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFR-----------------------YGNCR 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFR                       YGNCR
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSRVWPMVIGYLFFRIVFQSQIGSSS 161
           IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRS VWPMVIGYLFFRIVFQSQIGSSS
Sbjct: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS 180

[Close]

BLAST of Carg15717 vs. ExPASy TrEMBL
Match: A0A6J1HZS5 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita maxima OX=3661 GN=LOC111468407 PE=4 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 9.1e-76
Identity = 153/183 (83.61%), Postives = 156/183 (85.25%), Query Frame = 0


Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTR+QSGAT+LWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRNQSGATDLWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFR-----------------------YGNCR 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFR                       YG+CR
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGSCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSRVWPMVIGYLFFRIVFQSQIGSSS 161
           IPASSLRNGNF SQTPSVGLGPSEDISGSGRKSGRS VWPMVIGYLFFRIVFQSQIGS S
Sbjct: 121 IPASSLRNGNFPSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSPS 180

[Close]

BLAST of Carg15717 vs. ExPASy TrEMBL
Match: A0A6J1JNE0 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486269 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.6e-56
Identity = 121/172 (70.35%), Postives = 131/172 (76.16%), Query Frame = 0


Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           M  NFALFLLI+ IL  SAAE + PS + Q GATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MPINFALFLLIVPIL-CSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYF-----------------------RYGNCR 120
           GADCSPIQP GACYDASNVQNMASFAFNDYF                        YGNCR
Sbjct: 61  GADCSPIQPSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAVTSLNPSYGNCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSRVWPMVIGYLFFRIVF 150
            P+SSLRNGNFSSQ+ SVGLGPSED+SGSGR+S R  VWP+ IGYLFF+I+F
Sbjct: 121 FPSSSLRNGNFSSQSTSVGLGPSEDLSGSGRRSSRIWVWPIAIGYLFFKIMF 171

[Close]

BLAST of Carg15717 vs. ExPASy TrEMBL
Match: A0A6J1EY09 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437228 PE=4 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 5.2e-55
Identity = 118/172 (68.60%), Postives = 130/172 (75.58%), Query Frame = 0


Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           M  + ALFLLI LI+  SAAE + PS + Q GATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MEIDLALFLLIALII-CSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYF-----------------------RYGNCR 120
           G+DCSPIQP GACYDAS+VQ MASFAFNDYF                        YGNCR
Sbjct: 61  GSDCSPIQPSGACYDASSVQTMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSRVWPMVIGYLFFRIVF 150
            P+SSLRNGNFSSQ+ SVGLGPSED+SGSGRKSGR  VWP+ IGYLFF+I+F
Sbjct: 121 FPSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMF 171

[Close]

BLAST of Carg15717 vs. ExPASy TrEMBL
Match: A0A1S3BN12 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Cucumis melo OX=3656 GN=LOC103491346 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 4.4e-54
Identity = 118/172 (68.60%), Postives = 130/172 (75.58%), Query Frame = 0


Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSG-ATELWCVAKNNADDASLQSALDWACGA 60
           M  NF+LFLLILLI  ++A   R+PS RDQSG ATELWCVAKNNADDASLQSALDWACGA
Sbjct: 1   MPINFSLFLLILLIPCTAADPWRRPSIRDQSGAATELWCVAKNNADDASLQSALDWACGA 60

Query: 61  GGADCSPIQPGGACYDASNVQNMASFAFNDYFR-----------------------YGNC 120
           GGADCSPIQPGG+CYDA++VQNMASFAFNDYFR                       +GNC
Sbjct: 61  GGADCSPIQPGGSCYDATDVQNMASFAFNDYFRKHGMTDDSCFFQNSAAITSLNPSFGNC 120

Query: 121 RIPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSRVWPMVIGYLFFRIV 149
           R P+S LRNGNFSSQT SVGLGPSED+SGS    GR+ VWPMVIGYL  RI+
Sbjct: 121 RFPSSVLRNGNFSSQTTSVGLGPSEDMSGSAHNLGRAWVWPMVIGYLLLRIM 172

[Close]

BLAST of Carg15717 vs. TAIR 10
Match: AT3G58100.1 (plasmodesmata callose-binding protein 5 )

HSP 1 Score: 97.4 bits (241), Expect = 1.1e-20
Identity = 60/136 (44.12%), Postives = 77/136 (56.62%), Query Frame = 0


Query: 10  LILLILGSSAAESRQPSTRDQSG---ATELWCVAKNNADDASLQSALDWACGAGGADCSP 69
           L L IL ++AA     +T    G     ELWCVAKNNA+D+SLQ+A++WACG GGADC P
Sbjct: 11  LRLSILAATAAMLMVITTAQIGGQVIQVELWCVAKNNAEDSSLQTAIEWACGQGGADCGP 70

Query: 70  IQPGGACYDASNVQNMASFAFNDYFRYGNCRIPASSLRNG------NFSSQTPSVGLGPS 129
           IQ GG C D ++VQ MASF FN+Y+           L+NG      NF++      L PS
Sbjct: 71  IQQGGPCNDPTDVQKMASFVFNNYY-----------LKNGEEDEACNFNNNAALTSLNPS 130

Query: 130 EDI----SGSGRKSGR 133
           +      S  G  +GR
Sbjct: 131 QGTCKYPSSKGANNGR 135

[Close]

BLAST of Carg15717 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 77.4 bits (189), Expect = 1.2e-14
Identity = 44/113 (38.94%), Postives = 62/113 (54.87%), Query Frame = 0


Query: 37  WCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYFRY--- 96
           WC+AK NA   SLQ ALD+ACG GGADC  IQ G ACY+ + +++ ASFAFN Y++    
Sbjct: 148 WCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASFAFNSYYQKHPG 207

Query: 97  -------GNCRIPASSLRNG--NFSSQTPSVGLGPSEDISGSGRKSGRSRVWP 138
                  G  ++ ++    G  +FSS + +V   P   +S     S  S  +P
Sbjct: 208 SDSCNFGGAAQLTSTDPSKGSCHFSSSSGTVSTSPPSQMSPPDFSSPPSSTYP 260

[Close]

BLAST of Carg15717 vs. TAIR 10
Match: AT4G13600.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 74.7 bits (182), Expect = 7.5e-14
Identity = 53/143 (37.06%), Postives = 72/143 (50.35%), Query Frame = 0


Query: 7   LFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAGGADCSP 66
           L  L+LL+  SSAA               +WCVA+ +    +LQ+ALD+AC A GADC+P
Sbjct: 46  LSFLLLLLFRSSAA---------------MWCVARFDVTSQALQAALDYAC-AAGADCAP 105

Query: 67  IQPGGACYDASNVQNMASFAFNDYFR-----------------------YGNCRIPASSL 126
           IQP G C+  + VQ  AS+AFN YF+                       YG+C  P +S+
Sbjct: 106 IQPNGLCFLPNTVQAHASYAFNSYFQRAAMAPGSCNFAGTSTIAKTDPSYGSCVYP-NSV 165

[Close]

BLAST of Carg15717 vs. TAIR 10
Match: AT2G30933.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 2.2e-13
Identity = 40/84 (47.62%), Postives = 52/84 (61.90%), Query Frame = 0


Query: 37  WCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYFRYGNC 96
           WCVA+ N    +LQ+ALD+ACG GGADCS IQ GG CY+ ++++  ASFAFN Y  Y   
Sbjct: 82  WCVARENVAKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHASFAFNSY--YQKN 141

Query: 97  RIPASSLRNGNFSSQTPSVGLGPS 121
            IP+S     NF     ++   PS
Sbjct: 142 PIPSSC----NFDGTAITISADPS 159

[Close]

BLAST of Carg15717 vs. TAIR 10
Match: AT2G30933.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 2.2e-13
Identity = 40/84 (47.62%), Postives = 52/84 (61.90%), Query Frame = 0


Query: 37  WCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYFRYGNC 96
           WCVA+ N    +LQ+ALD+ACG GGADCS IQ GG CY+ ++++  ASFAFN Y  Y   
Sbjct: 82  WCVARENVAKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHASFAFNSY--YQKN 141

Query: 97  RIPASSLRNGNFSSQTPSVGLGPS 121
            IP+S     NF     ++   PS
Sbjct: 142 PIPSSC----NFDGTAITISADPS 159

The following BLAST results are available for this feature:

BLAST of Carg15717 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012010.1	2.9e-84	100.00	PLASMODESMATA CALLOSE-BINDING PROTEIN 5, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022952645.1	2.1e-79	86.89	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucurbita moschata]	[more]
XP_023554742.1	2.8e-79	86.34	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X2 [Cucurbita pepo subsp. p...	[more]
XP_022969385.1	1.9e-75	83.61	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucurbita maxima]	[more]
XP_023554741.1	7.1e-67	84.57	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita pepo subsp. p...	[more]

BLAST of Carg15717 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2K6	1.5e-19	44.12	PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5...	[more]
O65399	1.2e-11	51.72	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q9FJU9	3.4e-11	34.45	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
Q94CD8	4.4e-11	52.54	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]
Q8VYE5	9.8e-11	40.45	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]

BLAST of Carg15717 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GMC7	1.0e-79	86.89	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1HZS5	9.1e-76	83.61	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1JNE0	1.6e-56	70.35	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita maxima OX=3...	[more]
A0A6J1EY09	5.2e-55	68.60	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita moschata OX...	[more]
A0A1S3BN12	4.4e-54	68.60	PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Cucumis melo OX=3656 GN=LOC103491346 ...	[more]

BLAST of Carg15717 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G58100.1	1.1e-20	44.12	plasmodesmata callose-binding protein 5	[more]
AT1G29380.1	1.2e-14	38.94	Carbohydrate-binding X8 domain superfamily protein	[more]
AT4G13600.1	7.5e-14	37.06	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G30933.1	2.2e-13	47.62	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G30933.2	2.2e-13	47.62	Carbohydrate-binding X8 domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 36..98 e-value: 1.2E-17 score: 74.6
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 36..92 e-value: 2.6E-15 score: 56.9
None	No IPR available	GENE3D	1.20.58.1040		coord: 31..115 e-value: 2.4E-6 score: 29.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 111..131
None	No IPR available	PANTHER	PTHR31044:SF79	BETA-1,3 GLUCANASE	coord: 28..93
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 28..93

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg15717-RA	Carg15717-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
cellular_component	GO:0009506	plasmodesma