Carg15480 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15480
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description15-cis-zeta-carotene isomerase, chloroplastic
LocationCarg_Chr06: 10036496 .. 10039524 (+)
RNA-Seq ExpressionCarg15480
SyntenyCarg15480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTTGAAGCGCACCAATTTTCACAGATTCTTCTCCACTTTCCATTTCCCCAACCCCCTCAATCCTCATTTTGCCTTCTCCTCCCACTCTCCATTGACAACCACCTCCGATTACAGTGCTCTAATGGCGGTAGCCTCCGCCACGACCCTCTTTTTCCCCCCACTTCCCACCTCTTCTAACCAACCCCACCATCGCCATCGCCGTCTCGATGCTTTTACCCTCCGACCCTCTTCAATTTCCACCCCCAATTTCCTTCCCCAACTTCCCTTTTCCCTCAATTCCTCACCGATTCCCCGCCGTCGTCGATTTTCGGCTGAGGCTTCTATTGGAGACAGCGAGAGTGGCGGTTCGACCTCTACCTCGGATGACGACGGCCTTGTCGGCGAGGACGCTGCTGTTTTTAATCTTTCCGAGCAGAAATTGGCGTCGTGGGCTTATTTCACTGTGATTTTGGGGGTTGTTCTGTTCGTGTTGAATGTTGTTTGGATCGATAATTCTGCTGGGGGTGTTGGGAAGGCCTTCGTGGATGCTATTTCTGGACTTTCGGATAGTCATGAGGTACCCTTCTATATGATTTCTGTAGCTTGATTTCCTTTTTGGAGTATTAGAACATGCTGAAGACTGTATAGGAATACTTCCATTGCTAGATTTGCTGGATTGCAGGACAATATCATTTCGTTGTTCTTTGTCTTTAACATGCTGAAGATTGGTTTTGGTTGAAGCTAATGGGTGTTTATAGCTGGATGAACTGGCTTCCAATTTACTAAATTCCATTGCTAGCTGATGTTTAAATTTGGTGATTTTGGTGTATAAAAGATGAAACTAGAACTTTGGAACAATTGCATAAACCATCCCTAAACTATGGAGTTTGTAACAATCATACCCTTGAACTTTGAAGTGTTGCAATCGATCCGTATACTTTGTTAAGTGTAGCAATCAGCCCTTCCGATTTAACAAGAGACCTTAAGTCAAGAGGCATTCGGGAAGCGACTAGTCACTTCTAGCAACGCTTCTATGAGGCCGACCACTACATAAACAAAGAGATCCTTCAATGATTGCAGCACTAACTACTGGAGTGATTGATCGAACTATAAGTTCAAGAGTGTGATTGGAACGCTTAGCAAAGTTGAAGGGGCGGATTGATCTTAAAAGTTCAAAGGTGTAATGGTAACAAGTCCTATAGTCGAGGGGCGGCTTGTGCGATTTCCCCAAAACTTTTAAGATGAAAATCTTACCATCGACCGGGGAGGACGAACTGAGCATATGTGGTGCATGGCCTTGCTAAATGTCTGAATCTCAGTCTTGGAACTTTGTTATGCAGGTTGTGATGTTGGCCCTCATTTTCATTTTTGCTGTTGTCCACAGTGGTTTGGCTAGTCTCCGAGATCAGGGCGAGAAGCTCGTTGGAGAACGAGCTTTTCGGGTTCTGTTTGCAGGAGTTTCTCTTCCATTGGCTGTCAGCACTGTGGTAGGTTCCTTCCCTCTACTTTTCCCTTTATCTTCTCTGACTGCACACCCCCATATTGACTTGTTTACACTAAGGAGTTGTATTCAGGTGTACTTCATTAACCATCGATACGACGGGGTACAGTTATGGCAGCTCCACAGCATTCCCGGATTGCATCAACTCGTGTGGCTCAGTTCGTTTATCTCCTTCTTCTTCCTATACCCTTCTACTTTTAATCTGCTAGAAGTCGCTGCAGTCGATAAACCGAAAATGCACCTTTGGGAAACAGGCATCATGAGAATAACTAGGCATCCACAGGTATGTTTGTGTTTCATTATCTTGAATGTTCATTGCATTTCATGACAGTATCAGGGTTTCTTTGCGGTTTATTTGATTGCTTCGGCTTGAACCGTTGATGAACGTCGTCATTGGCATGTTAGATAAGTTTTCTTATGTCAAGTATATTCGGTTAGCTGCTGTAAAGAGCTCGAGTATGAAAGCTTAGCGACCAAAATAGAAGTCAAACTAAACTATTTTCCCGTAAAAGTCATGTTTAATCTTACGGAACAAATTCGTTTGAAGATTTGAAACAAGAAGATAGGGAGACGAAATGGATTTAATGTGTAACAGCTCAAGCCTAGTACTAGTAGATATTGTCCTCTTTGGGGTTTCCCTCAAAGTTTTTAAAACACGTCTACTAGGGAGAGGTTTCCACACCCTTTTAAAGAATACTTCGTTCTCCTCCCCAACCGATATGGGATCTCACAATCTACTCCCCTTTGGGGCTCAGCATCCTCACTGACACTCATTCTCTTCTCCAATCAATGTGGGACCCCTTAATCTAGCGTCCTTGCTGGCACAACCACCCCGTGTCTGGCTTTAATACCATTTGTAGCTGCCTAAGTCCACCGCTAGAAGATATTGTCCTCTTTGGGTTTTCCCTCAAGGTTTTAAAACTCGTCTGTTAGGAAGAGGTTTCCACACCCTTATAAAGAATGCTTCGTTCTCCTCCTCAACTGACGTGTGATCTCATATGGTGATTATCTGTTTCAAGATCGGATGTTGATATTCACGAGGGGAGAGGACAAACACGACCTTTTTATAAACACTGATCATACCAAATCTTGGTTTCTGTTTGAAGATGGTTGGACAGGTGATCTGGTGCCTTGCTCACACAGTCTGGATTGGGAACTCAGTTGCAGTAGCAGCTTCCATTGGGTTGATAGGACATCATCTGTTCGGGGTATGGAACGGGGACAGGAGGCTAGCAAAGCGATACGGGGCAGATTTTGAAGCGGTGAAAAGGCGAACGAGCGTCGTTCCATTTGCTGCCATTCTGGATGGTCGTCAAAAGTTGCCCGACGACTACTACAAGGAGTTCATTCGGTTGCCATATCTATCAATCACTGCACTGACCATAGGTGCCTACCTTGCCCACCCTCTCATGCAAGCTGCAAGCTTCAGGCTTCATTGGTAGACTTTATTTCTATGCAGCCGACTCACTTGTATTAGAACTATACATCACTGTATAAACTAAGGCACCAATGGTAGAGAAATTTGATCCCTTAGTTATAGCCTTATT

mRNA sequence

TCTTTGAAGCGCACCAATTTTCACAGATTCTTCTCCACTTTCCATTTCCCCAACCCCCTCAATCCTCATTTTGCCTTCTCCTCCCACTCTCCATTGACAACCACCTCCGATTACAGTGCTCTAATGGCGGTAGCCTCCGCCACGACCCTCTTTTTCCCCCCACTTCCCACCTCTTCTAACCAACCCCACCATCGCCATCGCCGTCTCGATGCTTTTACCCTCCGACCCTCTTCAATTTCCACCCCCAATTTCCTTCCCCAACTTCCCTTTTCCCTCAATTCCTCACCGATTCCCCGCCGTCGTCGATTTTCGGCTGAGGCTTCTATTGGAGACAGCGAGAGTGGCGGTTCGACCTCTACCTCGGATGACGACGGCCTTGTCGGCGAGGACGCTGCTGTTTTTAATCTTTCCGAGCAGAAATTGGCGTCGTGGGCTTATTTCACTGTGATTTTGGGGGTTGTTCTGTTCGTGTTGAATGTTGTTTGGATCGATAATTCTGCTGGGGGTGTTGGGAAGGCCTTCGTGGATGCTATTTCTGGACTTTCGGATAGTCATGAGGTTGTGATGTTGGCCCTCATTTTCATTTTTGCTGTTGTCCACAGTGGTTTGGCTAGTCTCCGAGATCAGGGCGAGAAGCTCGTTGGAGAACGAGCTTTTCGGGTTCTGTTTGCAGGAGTTTCTCTTCCATTGGCTGTCAGCACTGTGGTAGGTTCCTTCCCTCTACTTTTCCCTTTATCTTCTCTGACTGCACACCCCCATATTGACTTGTTTACACTAAGGAGTTGTATTCAGGTGTACTTCATTAACCATCGATACGACGGGGTACAGTTATGGCAGCTCCACAGCATTCCCGGATTGCATCAACTCGTGTGGCTCAGTTCGTTTATCTCCTTCTTCTTCCTATACCCTTCTACTTTTAATCTGCTAGAAGTCGCTGCAGTCGATAAACCGAAAATGCACCTTTGGGAAACAGGCATCATGAGAATAACTAGGCATCCACAGATGGTTGGACAGGTGATCTGGTGCCTTGCTCACACAGTCTGGATTGGGAACTCAGTTGCAGTAGCAGCTTCCATTGGGTTGATAGGACATCATCTGTTCGGGGTATGGAACGGGGACAGGAGGCTAGCAAAGCGATACGGGGCAGATTTTGAAGCGGTGAAAAGGCGAACGAGCGTCGTTCCATTTGCTGCCATTCTGGATGGTCGTCAAAAGTTGCCCGACGACTACTACAAGGAGTTCATTCGGTTGCCATATCTATCAATCACTGCACTGACCATAGGTGCCTACCTTGCCCACCCTCTCATGCAAGCTGCAAGCTTCAGGCTTCATTGGTAGACTTTATTTCTATGCAGCCGACTCACTTGTATTAGAACTATACATCACTGTATAAACTAAGGCACCAATGGTAGAGAAATTTGATCCCTTAGTTATAGCCTTATT

Coding sequence (CDS)

ATGGCGGTAGCCTCCGCCACGACCCTCTTTTTCCCCCCACTTCCCACCTCTTCTAACCAACCCCACCATCGCCATCGCCGTCTCGATGCTTTTACCCTCCGACCCTCTTCAATTTCCACCCCCAATTTCCTTCCCCAACTTCCCTTTTCCCTCAATTCCTCACCGATTCCCCGCCGTCGTCGATTTTCGGCTGAGGCTTCTATTGGAGACAGCGAGAGTGGCGGTTCGACCTCTACCTCGGATGACGACGGCCTTGTCGGCGAGGACGCTGCTGTTTTTAATCTTTCCGAGCAGAAATTGGCGTCGTGGGCTTATTTCACTGTGATTTTGGGGGTTGTTCTGTTCGTGTTGAATGTTGTTTGGATCGATAATTCTGCTGGGGGTGTTGGGAAGGCCTTCGTGGATGCTATTTCTGGACTTTCGGATAGTCATGAGGTTGTGATGTTGGCCCTCATTTTCATTTTTGCTGTTGTCCACAGTGGTTTGGCTAGTCTCCGAGATCAGGGCGAGAAGCTCGTTGGAGAACGAGCTTTTCGGGTTCTGTTTGCAGGAGTTTCTCTTCCATTGGCTGTCAGCACTGTGGTAGGTTCCTTCCCTCTACTTTTCCCTTTATCTTCTCTGACTGCACACCCCCATATTGACTTGTTTACACTAAGGAGTTGTATTCAGGTGTACTTCATTAACCATCGATACGACGGGGTACAGTTATGGCAGCTCCACAGCATTCCCGGATTGCATCAACTCGTGTGGCTCAGTTCGTTTATCTCCTTCTTCTTCCTATACCCTTCTACTTTTAATCTGCTAGAAGTCGCTGCAGTCGATAAACCGAAAATGCACCTTTGGGAAACAGGCATCATGAGAATAACTAGGCATCCACAGATGGTTGGACAGGTGATCTGGTGCCTTGCTCACACAGTCTGGATTGGGAACTCAGTTGCAGTAGCAGCTTCCATTGGGTTGATAGGACATCATCTGTTCGGGGTATGGAACGGGGACAGGAGGCTAGCAAAGCGATACGGGGCAGATTTTGAAGCGGTGAAAAGGCGAACGAGCGTCGTTCCATTTGCTGCCATTCTGGATGGTCGTCAAAAGTTGCCCGACGACTACTACAAGGAGTTCATTCGGTTGCCATATCTATCAATCACTGCACTGACCATAGGTGCCTACCTTGCCCACCCTCTCATGCAAGCTGCAAGCTTCAGGCTTCATTGGTAG

Protein sequence

MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRRRFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVVWIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRVLFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIWCLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILDGRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW
Homology
BLAST of Carg15480 vs. NCBI nr
Match: KAG7029085.1 (15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 802.0 bits (2070), Expect = 2.4e-228
Identity = 404/404 (100.00%), Postives = 404/404 (100.00%), Query Frame = 0

Query: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60
           MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR
Sbjct: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60

Query: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120
           RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV
Sbjct: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLH 240
           LFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLH
Sbjct: 181 LFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLH 240

Query: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300
           SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW
Sbjct: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300

Query: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360
           CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD
Sbjct: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360

Query: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 405
           GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW
Sbjct: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 404

BLAST of Carg15480 vs. NCBI nr
Match: XP_022932715.1 (15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita moschata] >KAG6597644.1 15-cis-zeta-carotene isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 728.8 bits (1880), Expect = 2.6e-206
Identity = 375/404 (92.82%), Postives = 375/404 (92.82%), Query Frame = 0

Query: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60
           MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR
Sbjct: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60

Query: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120
           RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV
Sbjct: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLH 240
           LFAGVSLPLAVSTV                             VYFINHRYDGVQLWQLH
Sbjct: 181 LFAGVSLPLAVSTV-----------------------------VYFINHRYDGVQLWQLH 240

Query: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300
           SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW
Sbjct: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300

Query: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360
           CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD
Sbjct: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360

Query: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 405
           GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW
Sbjct: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 375

BLAST of Carg15480 vs. NCBI nr
Match: XP_022972107.1 (15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 716.5 bits (1848), Expect = 1.3e-202
Identity = 369/404 (91.34%), Postives = 370/404 (91.58%), Query Frame = 0

Query: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60
           MAVASATTLFF P PTSSNQPHHRHRR DAFTLRPSSISTPNFLPQL FSLNSSPIPRRR
Sbjct: 1   MAVASATTLFFSPFPTSSNQPHHRHRRPDAFTLRPSSISTPNFLPQLSFSLNSSPIPRRR 60

Query: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120
           RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASW YFTVILGVVLFVLNVV
Sbjct: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWVYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAFVDA+SGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLH 240
           LFAGVSLPLAVSTV                             VYFINHRYDGVQLWQLH
Sbjct: 181 LFAGVSLPLAVSTV-----------------------------VYFINHRYDGVQLWQLH 240

Query: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300
           SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW
Sbjct: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300

Query: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360
           CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD
Sbjct: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360

Query: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 405
           GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW
Sbjct: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 375

BLAST of Carg15480 vs. NCBI nr
Match: XP_023540701.1 (15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 709.5 bits (1830), Expect = 1.6e-200
Identity = 367/406 (90.39%), Postives = 370/406 (91.13%), Query Frame = 0

Query: 1   MAVASATTLFFPPLPTSSNQPH--HRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPR 60
           MAVASATTLFFPP P+ SNQPH  HRHRR DA TLRPSSISTPN LPQLPFSLNSSPIPR
Sbjct: 1   MAVASATTLFFPPFPSPSNQPHHRHRHRRPDALTLRPSSISTPNVLPQLPFSLNSSPIPR 60

Query: 61  RRRFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLN 120
           RRRFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLS+QKLASWAYFTVILGVVLFVLN
Sbjct: 61  RRRFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSDQKLASWAYFTVILGVVLFVLN 120

Query: 121 VVWIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAF 180
           VVWIDNSAGGVGKAFVDA+SGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAF
Sbjct: 121 VVWIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAF 180

Query: 181 RVLFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQ 240
           RVLFAGVSLPLAVSTV                             VYFINHRYDGVQLWQ
Sbjct: 181 RVLFAGVSLPLAVSTV-----------------------------VYFINHRYDGVQLWQ 240

Query: 241 LHSIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQV 300
           LHSIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQV
Sbjct: 241 LHSIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQV 300

Query: 301 IWCLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAI 360
           IWCLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAI
Sbjct: 301 IWCLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAI 360

Query: 361 LDGRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 405
           LDGRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW
Sbjct: 361 LDGRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 377

BLAST of Carg15480 vs. NCBI nr
Match: XP_038882546.1 (15-cis-zeta-carotene isomerase, chloroplastic [Benincasa hispida])

HSP 1 Score: 637.9 bits (1644), Expect = 6.0e-179
Identity = 330/404 (81.68%), Postives = 346/404 (85.64%), Query Frame = 0

Query: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60
           MAVASAT  F PP  +S      RHRR DA TLRP SISTPN   QLPFS NS+PI RR 
Sbjct: 1   MAVASATPPFSPPFLSS-----RRHRRPDATTLRPFSISTPNCPLQLPFSFNSTPISRRP 60

Query: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120
           RF+AEASIGDSESGGSTS SDD G VGEDAA F+LS+QKL SW YFTVILGVVLFVLNVV
Sbjct: 61  RFAAEASIGDSESGGSTSVSDDGGFVGEDAAAFDLSDQKLTSWVYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAFVDA+SG+SDSHEVVML L FIFA+VHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFVDAVSGISDSHEVVMLLLTFIFAIVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLH 240
           LFAGVSLPLAVSTV                             VYFINHRYDGVQLWQL 
Sbjct: 181 LFAGVSLPLAVSTV-----------------------------VYFINHRYDGVQLWQLQ 240

Query: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300
           S+PGLHQLVWLSSF+SFFFLYPSTFNLLEVAAVDKPKMHLWETGI+RITRHPQMVGQV+W
Sbjct: 241 SVPGLHQLVWLSSFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMW 300

Query: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360
           CLAHT+WIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTS+VPFAAI+D
Sbjct: 301 CLAHTIWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSIVPFAAIVD 360

Query: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 405
           GRQKLPDDYYKEF+RLPYLSITALTIGAYLAHPLMQAASFRLHW
Sbjct: 361 GRQKLPDDYYKEFVRLPYLSITALTIGAYLAHPLMQAASFRLHW 370

BLAST of Carg15480 vs. ExPASy Swiss-Prot
Match: Q9SAC0 (15-cis-zeta-carotene isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=Z-ISO PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 4.7e-134
Identity = 235/355 (66.20%), Postives = 278/355 (78.31%), Query Frame = 0

Query: 50  SLNSSPIPRRRRFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVI 109
           SL SS  P  R+    +++ + +   S S S    L+GED+A F L +QKL SW YF V+
Sbjct: 43  SLLSSSSPVIRKILVRSTLREDQPIASDSESSPTLLIGEDSAAFELGKQKLVSWVYFGVV 102

Query: 110 LGVVLFVLNVVWIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQG 169
           LGVVLF+LNVVWIDNS  G GK+F+DA+S +S S EV ML LI IFA+VHSGLASLRD G
Sbjct: 103 LGVVLFILNVVWIDNST-GFGKSFIDAVSNISGSPEVAMLMLILIFAIVHSGLASLRDIG 162

Query: 170 EKLVGERAFRVLFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINH 229
           EKL+GERAFRVLFAG+SLPLA+ST+                             VYFINH
Sbjct: 163 EKLIGERAFRVLFAGISLPLAMSTI-----------------------------VYFINH 222

Query: 230 RYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRIT 289
           RYDG QLWQL  +PG+H+ +W+++F+SFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRIT
Sbjct: 223 RYDGSQLWQLQGVPGVHEAIWVANFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRIT 282

Query: 290 RHPQMVGQVIWCLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRR 349
           RHPQMVGQ++WCLAHT+WIGN+VA +AS+GLI HHLFG WNGDRRLAKRYG DFE++K+R
Sbjct: 283 RHPQMVGQIVWCLAHTLWIGNTVAASASLGLIAHHLFGAWNGDRRLAKRYGEDFESIKKR 342

Query: 350 TSVVPFAAILDGRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 405
           TSV+PFAAI +GRQ LP+DYYKEF+RLPYL+ITALT+GAY AHPLMQ ASFRLHW
Sbjct: 343 TSVIPFAAIFEGRQVLPEDYYKEFVRLPYLAITALTVGAYFAHPLMQGASFRLHW 367

BLAST of Carg15480 vs. ExPASy Swiss-Prot
Match: B4FHU1 (15-cis-zeta-carotene isomerase, chloroplastic OS=Zea mays OX=4577 PE=1 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 1.8e-125
Identity = 224/336 (66.67%), Postives = 258/336 (76.79%), Query Frame = 0

Query: 69  GDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVVWIDNSAGG 128
           GD   GG         LVGED+A F L +Q +ASWAYF  ILG VL  LNV+WID S  G
Sbjct: 68  GDESGGGPV-------LVGEDSAAFELKDQSVASWAYFAGILGAVLVALNVLWIDPST-G 127

Query: 129 VGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRVLFAGVSLP 188
           VG  F+DA++ +SDSHEVVML L  IFAVVHSG+ASLR+ GEK+VGER +RVLFAG+SLP
Sbjct: 128 VGTKFLDAVASVSDSHEVVMLLLTIIFAVVHSGMASLRESGEKIVGERVYRVLFAGISLP 187

Query: 189 LAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLHSIPGLHQL 248
           LAV+T+                             VYFINHRYDG QLWQ+  I G+H+L
Sbjct: 188 LAVTTI-----------------------------VYFINHRYDGTQLWQVQGITGIHEL 247

Query: 249 VWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIWCLAHTVWI 308
           +W SSFISFFFLYPSTFNLLEVAAVDKPK+H+WETGIMRITRHPQMVGQVIWCLAHT+WI
Sbjct: 248 LWFSSFISFFFLYPSTFNLLEVAAVDKPKLHMWETGIMRITRHPQMVGQVIWCLAHTLWI 307

Query: 309 GNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILDGRQKLPDD 368
           GNSVAVAAS+GLI HHLFG WNGDRRL  RYG  FE +K+RTSV+PFAAI+DGRQKLP D
Sbjct: 308 GNSVAVAASVGLISHHLFGAWNGDRRLLSRYGEAFEVLKKRTSVMPFAAIIDGRQKLPKD 366

Query: 369 YYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 405
           Y+KEF RLPY++IT LT+GAY AHPLMQA+S++L W
Sbjct: 368 YHKEFFRLPYVAITMLTLGAYFAHPLMQASSYQLPW 366

BLAST of Carg15480 vs. ExPASy TrEMBL
Match: A0A6J1F2X1 (15-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111439180 PE=4 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.3e-206
Identity = 375/404 (92.82%), Postives = 375/404 (92.82%), Query Frame = 0

Query: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60
           MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR
Sbjct: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60

Query: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120
           RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV
Sbjct: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLH 240
           LFAGVSLPLAVSTV                             VYFINHRYDGVQLWQLH
Sbjct: 181 LFAGVSLPLAVSTV-----------------------------VYFINHRYDGVQLWQLH 240

Query: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300
           SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW
Sbjct: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300

Query: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360
           CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD
Sbjct: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360

Query: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 405
           GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW
Sbjct: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 375

BLAST of Carg15480 vs. ExPASy TrEMBL
Match: A0A6J1I3V9 (15-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470740 PE=4 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 6.4e-203
Identity = 369/404 (91.34%), Postives = 370/404 (91.58%), Query Frame = 0

Query: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60
           MAVASATTLFF P PTSSNQPHHRHRR DAFTLRPSSISTPNFLPQL FSLNSSPIPRRR
Sbjct: 1   MAVASATTLFFSPFPTSSNQPHHRHRRPDAFTLRPSSISTPNFLPQLSFSLNSSPIPRRR 60

Query: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120
           RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASW YFTVILGVVLFVLNVV
Sbjct: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWVYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAFVDA+SGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLH 240
           LFAGVSLPLAVSTV                             VYFINHRYDGVQLWQLH
Sbjct: 181 LFAGVSLPLAVSTV-----------------------------VYFINHRYDGVQLWQLH 240

Query: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300
           SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW
Sbjct: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300

Query: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360
           CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD
Sbjct: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360

Query: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 405
           GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW
Sbjct: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 375

BLAST of Carg15480 vs. ExPASy TrEMBL
Match: A0A0A0KXF1 (NnrU domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G015720 PE=4 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 2.3e-176
Identity = 326/404 (80.69%), Postives = 342/404 (84.65%), Query Frame = 0

Query: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60
           MAVASAT  F PP  +S   P HRHRR DA + RP SISTPN L QLP SLNS  I RR 
Sbjct: 1   MAVASATPPFSPPFLSS---PRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRP 60

Query: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120
           R  AEASIGD ESGGSTS SDD+G VGEDAA F+LSEQKL SW YFTVILGVVLFVLNVV
Sbjct: 61  RLVAEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAF+DA+SG+SDSHEVVML LIFIFA+VHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLH 240
           LFAGVSLPLAVSTV                             VYFINHRYDGVQLWQL 
Sbjct: 181 LFAGVSLPLAVSTV-----------------------------VYFINHRYDGVQLWQLQ 240

Query: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300
           S+P LHQLVWLSSF+SF FLYPSTFNLLEVAAVDKPKMHLWETGI+RITRHPQMVGQVIW
Sbjct: 241 SVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVIW 300

Query: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360
           CLAHT+WIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVK RTS++PFAAI+D
Sbjct: 301 CLAHTIWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKSRTSIIPFAAIVD 360

Query: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 405
           GRQKLPDDYYKEF+RLPYLSITALTIGAYLAHPLMQAASFRLHW
Sbjct: 361 GRQKLPDDYYKEFLRLPYLSITALTIGAYLAHPLMQAASFRLHW 372

BLAST of Carg15480 vs. ExPASy TrEMBL
Match: A0A1S3BYR9 (15-cis-zeta-carotene isomerase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494534 PE=4 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 3.0e-176
Identity = 326/404 (80.69%), Postives = 344/404 (85.15%), Query Frame = 0

Query: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60
           MAVASAT  F PP  +S      RHRR DA +LRP SIS PN L QLP SLNS  I RR 
Sbjct: 1   MAVASATPPFSPPYLSSP-----RHRRPDAISLRPFSISAPNCLLQLPLSLNSLHIFRRP 60

Query: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120
           RF+AEASIGD ESGGSTS SDD+GLVGEDAAVF+LSEQKL SW YFTVILGVVLFVLNVV
Sbjct: 61  RFAAEASIGDRESGGSTSVSDDEGLVGEDAAVFDLSEQKLTSWVYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAF+DA+SG+SDSHEVVML L  IFA+VHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFLDAVSGISDSHEVVMLLLTLIFAIVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLH 240
           LFAGVSLPLAVSTV                             VYFINHRYDGVQLWQL 
Sbjct: 181 LFAGVSLPLAVSTV-----------------------------VYFINHRYDGVQLWQLQ 240

Query: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300
           S+PGLHQLVWLSSF+SFFFLYPSTFNLLEVAAVDKPKMHLWETGI+RITRHPQMVGQVIW
Sbjct: 241 SVPGLHQLVWLSSFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVIW 300

Query: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360
           CLAHT+WIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYG DFEAV+RRTS++PFAAI+D
Sbjct: 301 CLAHTIWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGVDFEAVQRRTSIIPFAAIVD 360

Query: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 405
           GRQKLPDDYYKEF+RLPYLSITALTIGAYLAHPLMQAASFRLHW
Sbjct: 361 GRQKLPDDYYKEFLRLPYLSITALTIGAYLAHPLMQAASFRLHW 370

BLAST of Carg15480 vs. ExPASy TrEMBL
Match: A0A5A7TMA2 (15-cis-zeta-carotene isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003540 PE=4 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 3.4e-172
Identity = 322/404 (79.70%), Postives = 339/404 (83.91%), Query Frame = 0

Query: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60
           MAVASAT  F PP  +S      RHRR DA +LRP SIS PN L QLP SLNS  I RR 
Sbjct: 1   MAVASATPPFSPPYLSSP-----RHRRPDAISLRPFSISAPNCLLQLPLSLNSLHIFRRP 60

Query: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120
           RF+AEASIGD ESGGSTS SDD+GLVGEDAAVF+LSEQKL SW YFTVILGVVLFVLNVV
Sbjct: 61  RFAAEASIGDRESGGSTSVSDDEGLVGEDAAVFDLSEQKLTSWVYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAF+DA+SG+SDSHEVVML L  IFA+VHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFLDAVSGISDSHEVVMLLLTLIFAIVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLH 240
           LFAGVSLPLAVSTV                             VYFINHRYDGVQLWQL 
Sbjct: 181 LFAGVSLPLAVSTV-----------------------------VYFINHRYDGVQLWQLQ 240

Query: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300
           S+PGLHQLVWLSSF+SFFFLYPSTFNLLEVAAVDKPKMHLWETGI+RITRHP    QVIW
Sbjct: 241 SVPGLHQLVWLSSFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIIRITRHP----QVIW 300

Query: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360
           CLAHT+WIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYG DFEAVKRRTS++PFAAI+D
Sbjct: 301 CLAHTIWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGVDFEAVKRRTSIIPFAAIVD 360

Query: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 405
           GRQKLPDDYYKEF+RLPYLSITALTIGAYLAHPLMQ ASFRLHW
Sbjct: 361 GRQKLPDDYYKEFLRLPYLSITALTIGAYLAHPLMQVASFRLHW 366

BLAST of Carg15480 vs. TAIR 10
Match: AT1G10830.1 (15-cis-zeta-carotene isomerase )

HSP 1 Score: 479.2 bits (1232), Expect = 3.3e-135
Identity = 235/355 (66.20%), Postives = 278/355 (78.31%), Query Frame = 0

Query: 50  SLNSSPIPRRRRFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVI 109
           SL SS  P  R+    +++ + +   S S S    L+GED+A F L +QKL SW YF V+
Sbjct: 43  SLLSSSSPVIRKILVRSTLREDQPIASDSESSPTLLIGEDSAAFELGKQKLVSWVYFGVV 102

Query: 110 LGVVLFVLNVVWIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQG 169
           LGVVLF+LNVVWIDNS  G GK+F+DA+S +S S EV ML LI IFA+VHSGLASLRD G
Sbjct: 103 LGVVLFILNVVWIDNST-GFGKSFIDAVSNISGSPEVAMLMLILIFAIVHSGLASLRDIG 162

Query: 170 EKLVGERAFRVLFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINH 229
           EKL+GERAFRVLFAG+SLPLA+ST+                             VYFINH
Sbjct: 163 EKLIGERAFRVLFAGISLPLAMSTI-----------------------------VYFINH 222

Query: 230 RYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRIT 289
           RYDG QLWQL  +PG+H+ +W+++F+SFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRIT
Sbjct: 223 RYDGSQLWQLQGVPGVHEAIWVANFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRIT 282

Query: 290 RHPQMVGQVIWCLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRR 349
           RHPQMVGQ++WCLAHT+WIGN+VA +AS+GLI HHLFG WNGDRRLAKRYG DFE++K+R
Sbjct: 283 RHPQMVGQIVWCLAHTLWIGNTVAASASLGLIAHHLFGAWNGDRRLAKRYGEDFESIKKR 342

Query: 350 TSVVPFAAILDGRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 405
           TSV+PFAAI +GRQ LP+DYYKEF+RLPYL+ITALT+GAY AHPLMQ ASFRLHW
Sbjct: 343 TSVIPFAAIFEGRQVLPEDYYKEFVRLPYLAITALTVGAYFAHPLMQGASFRLHW 367

BLAST of Carg15480 vs. TAIR 10
Match: AT1G10830.2 (15-cis-zeta-carotene isomerase )

HSP 1 Score: 283.1 bits (723), Expect = 3.5e-76
Identity = 149/253 (58.89%), Postives = 180/253 (71.15%), Query Frame = 0

Query: 50  SLNSSPIPRRRRFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVI 109
           SL SS  P  R+    +++ + +   S S S    L+GED+A F L +QKL SW YF V+
Sbjct: 43  SLLSSSSPVIRKILVRSTLREDQPIASDSESSPTLLIGEDSAAFELGKQKLVSWVYFGVV 102

Query: 110 LGVVLFVLNVVWIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQG 169
           LGVVLF+LNVVWIDNS  G GK+F+DA+S +S S EV ML LI IFA+VHSGLASLRD G
Sbjct: 103 LGVVLFILNVVWIDNST-GFGKSFIDAVSNISGSPEVAMLMLILIFAIVHSGLASLRDIG 162

Query: 170 EKLVGERAFRVLFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINH 229
           EKL+GERAFRVLFAG+SLPLA+ST+                             VYFINH
Sbjct: 163 EKLIGERAFRVLFAGISLPLAMSTI-----------------------------VYFINH 222

Query: 230 RYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRIT 289
           RYDG QLWQL  +PG+H+ +W+++F+SFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRIT
Sbjct: 223 RYDGSQLWQLQGVPGVHEAIWVANFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRIT 265

Query: 290 RHPQMVGQVIWCL 303
           RHPQ++   + CL
Sbjct: 283 RHPQVLNISLMCL 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029085.12.4e-228100.0015-cis-zeta-carotene isomerase, chloroplastic [Cucurbita argyrosperma subsp. arg... [more]
XP_022932715.12.6e-20692.8215-cis-zeta-carotene isomerase, chloroplastic [Cucurbita moschata] >KAG6597644.1... [more]
XP_022972107.11.3e-20291.3415-cis-zeta-carotene isomerase, chloroplastic [Cucurbita maxima][more]
XP_023540701.11.6e-20090.3915-cis-zeta-carotene isomerase, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_038882546.16.0e-17981.6815-cis-zeta-carotene isomerase, chloroplastic [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9SAC04.7e-13466.2015-cis-zeta-carotene isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
B4FHU11.8e-12566.6715-cis-zeta-carotene isomerase, chloroplastic OS=Zea mays OX=4577 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F2X11.3e-20692.8215-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1I3V96.4e-20391.3415-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A0A0KXF12.3e-17680.69NnrU domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G015720 PE=4 S... [more]
A0A1S3BYR93.0e-17680.6915-cis-zeta-carotene isomerase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5A7TMA23.4e-17279.7015-cis-zeta-carotene isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
Match NameE-valueIdentityDescription
AT1G10830.13.3e-13566.2015-cis-zeta-carotene isomerase [more]
AT1G10830.23.5e-7658.8915-cis-zeta-carotene isomerase [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009915NnrU domainPFAMPF07298NnrUcoord: 221..395
e-value: 5.8E-39
score: 133.8
coord: 147..196
e-value: 1.1E-6
score: 28.3
NoneNo IPR availableGENE3D1.20.120.1630coord: 141..355
e-value: 4.9E-9
score: 38.2
NoneNo IPR availablePANTHERPTHR3598815-CIS-ZETA-CAROTENE ISOMERASE, CHLOROPLASTICcoord: 58..195
coord: 222..404

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15480-RACarg15480-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016853 isomerase activity