Carg15462 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg15462
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	WRKY1
Location	Carg_Chr06: 10118646 .. 10119760 (-)
RNA-Seq Expression	Carg15462
Synteny	Carg15462

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTGATTTCTCGAGCTTCCGAGCCCACCGGAATCCATGGAAGTCATTTCCGGGAGGCGGGCATCTCCGGTGGGTTTCCGTTGTTTCCCGACGTGTCGGGGAGCTACATGTCTGTGGGTACGAGGAGTAGTATGAAGGAAGATATGGTTTCGGTTAGTAAAGGGTCAGCGGTGGAGGTGGTGGAGGGAGGAGCGGAGACGACGGAGATGGCGGCGGCGAGACAGGGAGATAATGATAAGAAAAAGAAGACGAGAAGCCGGAGATTTGCTTTTCAAACAAGGAGTCAAGTTGATATTCTTGATGATGGATATCGATGGAGAAAGTATGGACAGAAGGGTGTGAAGAACAACAAATTCCCAAGGTAACTTAATTAGTCAATAATTATTTCTTAGCATTAAAATCAACGCCTTAAAAGTCAAATTTTATGGTTAAGTATTGAATAATACCTCTAATTATGAGTAGAAATGTCTTTTAGAACCGTCACAAATCATATAAACATTAAAAGAAAGAGTCGAGAGAGATTTCTCTCCATTAACTAAATAAGATTGGGTACAGGGATTATATTTCCCGTCCCTGACCCTTACTACACTGCACTCTAATAGAGAAAAACAACCCGATTCTTGAAAAATTCTCGTCTCGATCCCTGTAAGAATAAATGAAAAATTTTGTGTGAATAGAGAATGAAATAGGAGGCGGAGACGGGGAACCCTAAGCACACTGCACCCTAATAGAGAAAAACAACCCAATTCTCGGAAAATTCTTGTTGGATCCCTGTAAGAATAAATGAAAATTTTTGTGAGAATAGATAATGAAATAGGAGGCGAGAACGAGAATGAGAACAGAGAAAACTTTTGTACTTCGATCGTGTGTACTGAATTATGAATTATTTGTTTGGTATTATATGTTGTAAGAAAAGGCTTGGTTAATGAATTGCAGAAGCTACTATAAATGCACACACCAAGGGTGCAAGGTGAAGAAGCAAGTGCAAAGGCTAACAAGAGATGAAGGAGTGGTGGTGACAACTTACGAAGGCATTCACTCTCATCCCATTCAGAAATCCACAGATAACTTCGAGCATATTTTGACCCAGATGCAAATTTACACTTCTTATTGA

mRNA sequence

ATGCTGATTTCTCGAGCTTCCGAGCCCACCGGAATCCATGGAAGTCATTTCCGGGAGGCGGGCATCTCCGGTGGGTTTCCGTTGTTTCCCGACGTGTCGGGGAGCTACATGTCTGTGGGTACGAGGAGTAGTATGAAGGAAGATATGGTTTCGGTTAGTAAAGGGTCAGCGGTGGAGGTGGTGGAGGGAGGAGCGGAGACGACGGAGATGGCGGCGGCGAGACAGGGAGATAATGATAAGAAAAAGAAGACGAGAAGCCGGAGATTTGCTTTTCAAACAAGGAGTCAAGTTGATATTCTTGATGATGGATATCGATGGAGAAAGTATGGACAGAAGGGTGTGAAGAACAACAAATTCCCAAGAAGCTACTATAAATGCACACACCAAGGGTGCAAGGTGAAGAAGCAAGTGCAAAGGCTAACAAGAGATGAAGGAGTGGTGGTGACAACTTACGAAGGCATTCACTCTCATCCCATTCAGAAATCCACAGATAACTTCGAGCATATTTTGACCCAGATGCAAATTTACACTTCTTATTGA

Coding sequence (CDS)

ATGCTGATTTCTCGAGCTTCCGAGCCCACCGGAATCCATGGAAGTCATTTCCGGGAGGCGGGCATCTCCGGTGGGTTTCCGTTGTTTCCCGACGTGTCGGGGAGCTACATGTCTGTGGGTACGAGGAGTAGTATGAAGGAAGATATGGTTTCGGTTAGTAAAGGGTCAGCGGTGGAGGTGGTGGAGGGAGGAGCGGAGACGACGGAGATGGCGGCGGCGAGACAGGGAGATAATGATAAGAAAAAGAAGACGAGAAGCCGGAGATTTGCTTTTCAAACAAGGAGTCAAGTTGATATTCTTGATGATGGATATCGATGGAGAAAGTATGGACAGAAGGGTGTGAAGAACAACAAATTCCCAAGAAGCTACTATAAATGCACACACCAAGGGTGCAAGGTGAAGAAGCAAGTGCAAAGGCTAACAAGAGATGAAGGAGTGGTGGTGACAACTTACGAAGGCATTCACTCTCATCCCATTCAGAAATCCACAGATAACTTCGAGCATATTTTGACCCAGATGCAAATTTACACTTCTTATTGA

Protein sequence

MLISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSKGSAVEVVEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY

Homology

[Close]

BLAST of Carg15462 vs. NCBI nr
Match: KAG7029102.1 (putative WRKY transcription factor 75, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 358.2 bits (918), Expect = 4.1e-95
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 0


Query: 1   MLISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSKGSAVEV 60
           MLISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSKGSAVEV
Sbjct: 1   MLISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSKGSAVEV 60

Query: 61  VEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP 120
           VEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP
Sbjct: 61  VEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP 120

Query: 121 RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 180
           RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY
Sbjct: 121 RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 179

[Close]

BLAST of Carg15462 vs. NCBI nr
Match: XP_022932710.1 (probable WRKY transcription factor 75 [Cucurbita moschata])

HSP 1 Score: 356.7 bits (914), Expect = 1.2e-94
Identity = 178/179 (99.44%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MLISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSKGSAVEV 60
           MLISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSKGSAVEV
Sbjct: 23  MLISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSKGSAVEV 82

Query: 61  VEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP 120
           VEGGAETTEM AARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP
Sbjct: 83  VEGGAETTEMTAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP 142

Query: 121 RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 180
           RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY
Sbjct: 143 RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 201

[Close]

BLAST of Carg15462 vs. NCBI nr
Match: XP_023539084.1 (probable WRKY transcription factor 75 isoform X1 [Cucurbita pepo subsp. pepo] >AXF54192.1 WRKY1 [Cucurbita pepo])

HSP 1 Score: 349.0 bits (894), Expect = 2.5e-92
Identity = 175/179 (97.77%), Postives = 175/179 (97.77%), Query Frame = 0


Query: 1   MLISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSKGSAVEV 60
           MLISRASEPTGIHGS FREA ISGGFPLFPDVSGSYMSVGTRSS KEDMVSVSKGSAVEV
Sbjct: 23  MLISRASEPTGIHGSQFREAAISGGFPLFPDVSGSYMSVGTRSSTKEDMVSVSKGSAVEV 82

Query: 61  VEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP 120
           VEGGAETTEM AARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP
Sbjct: 83  VEGGAETTEMTAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP 142

Query: 121 RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 180
           RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY
Sbjct: 143 RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 201

[Close]

BLAST of Carg15462 vs. NCBI nr
Match: XP_022972157.1 (probable WRKY transcription factor 75 [Cucurbita maxima])

HSP 1 Score: 341.7 bits (875), Expect = 3.9e-90
Identity = 175/183 (95.63%), Postives = 175/183 (95.63%), Query Frame = 0


Query: 2   LISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSK-----GS 61
           LISRASEPTGIHGSHFREAGISGGFPLFPDVS SYMSV TRSSMKEDMVSVSK     GS
Sbjct: 24  LISRASEPTGIHGSHFREAGISGGFPLFPDVSRSYMSVDTRSSMKEDMVSVSKGFHADGS 83

Query: 62  AVEVVEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKN 121
           A EVVEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKN
Sbjct: 84  AGEVVEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKN 143

Query: 122 NKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIY 180
           NKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIY
Sbjct: 144 NKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIY 203

[Close]

BLAST of Carg15462 vs. NCBI nr
Match: XP_038902977.1 (probable WRKY transcription factor 50 [Benincasa hispida])

HSP 1 Score: 245.7 bits (626), Expect = 2.9e-61
Identity = 136/195 (69.74%), Postives = 147/195 (75.38%), Query Frame = 0


Query: 1   MLISRASEPTGIH-GSHFRE-AGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSKGSAV 60
           ML SRASE  G H G H+R+   +SGGFPL  DV G  MS    SSMK+D V VS   + 
Sbjct: 23  MLFSRASELVGSHGGDHYRQPPPVSGGFPLSRDVLGDDMSFDASSSMKDDDVIVSLSKSF 82

Query: 61  EVVEG------GAETTEMAAA--------RQGDNDKKKKTRSRRFAFQTRSQVDILDDGY 120
            V E       G E  E+A A         +GD+DKKKK RSRRFAFQTRSQVDILDDGY
Sbjct: 83  RVDESARIGVDGLEKKEVATAGATTTTGTGRGDHDKKKKMRSRRFAFQTRSQVDILDDGY 142

Query: 121 RWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTD 180
           RWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPI+KSTD
Sbjct: 143 RWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIEKSTD 202

[Close]

BLAST of Carg15462 vs. ExPASy Swiss-Prot
Match: Q9FYA2 (Probable WRKY transcription factor 75 OS=Arabidopsis thaliana OX=3702 GN=WRKY75 PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 8.4e-43
Identity = 87/138 (63.04%), Postives = 109/138 (78.99%), Query Frame = 0


Query: 44  SMKEDMVSVSKGSAVEVVEGGAETTEMAA--ARQGDNDKKKKTRSRRFAFQTRSQVDILD 103
           S+    +S+   S  E+ +G  E++++ +    +     KKK + +R+AFQTRSQVDILD
Sbjct: 8   SLYAPFLSLKSHSKPELHQGEEESSKVRSEGCSKSVESSKKKGKKQRYAFQTRSQVDILD 67

Query: 104 DGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQK 163
           DGYRWRKYGQK VKNNKFPRSYY+CT+ GC VKKQVQRLT D+ VVVTTYEG+HSHPI+K
Sbjct: 68  DGYRWRKYGQKAVKNNKFPRSYYRCTYGGCNVKKQVQRLTVDQEVVVTTYEGVHSHPIEK 127

Query: 164 STDNFEHILTQMQIYTSY 180
           ST+NFEHILTQMQIY+S+
Sbjct: 128 STENFEHILTQMQIYSSF 145

[Close]

BLAST of Carg15462 vs. ExPASy Swiss-Prot
Match: Q9S763 (Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 2.8e-38
Identity = 77/119 (64.71%), Postives = 93/119 (78.15%), Query Frame = 0


Query: 61  VEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP 120
           V+  A+ T  ++  +  + KKKK R  R+AFQTRSQVDILDDGYRWRKYGQK VKNN FP
Sbjct: 26  VDNSAQPT-TSSEEKPRSKKKKKEREARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFP 85

Query: 121 RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 180
           RSYYKCT +GC+VKKQVQR   DEGVVVTTY+G+H+H + K +DNF HILTQM I+  +
Sbjct: 86  RSYYKCTEEGCRVKKQVQRQWGDEGVVVTTYQGVHTHAVDKPSDNFHHILTQMHIFPPF 143

[Close]

BLAST of Carg15462 vs. ExPASy Swiss-Prot
Match: Q9FFS3 (Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.6e-28
Identity = 60/106 (56.60%), Postives = 77/106 (72.64%), Query Frame = 0


Query: 75  QGDNDKKKKTRS-RRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKV 134
           +G   K+K++R   R AF TRS  D+LDDGYRWRKYGQK VK+N  PRSYY+CT+  C V
Sbjct: 71  KGKELKEKRSRKVPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTCNV 130

Query: 135 KKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 180
           KKQVQRL +D  VVVTTYEG+H+HP +K  +    +L Q+Q  +S+
Sbjct: 131 KKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLNPLLRQLQFLSSF 176

[Close]

BLAST of Carg15462 vs. ExPASy Swiss-Prot
Match: Q8GY11 (Probable WRKY transcription factor 43 OS=Arabidopsis thaliana OX=3702 GN=WRKY43 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 7.6e-28
Identity = 55/103 (53.40%), Postives = 76/103 (73.79%), Query Frame = 0


Query: 77  DNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQ 136
           D+ + KK ++ RF+F+T+S  DILDDGYRWRKYGQK VKN+ +PRSYY+CT   C VKKQ
Sbjct: 6   DSSRDKKMKNPRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQ 65

Query: 137 VQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 180
           VQRL+++  +V TTYEGIH+HP ++       +L Q+Q  + +
Sbjct: 66  VQRLSKETSIVETTYEGIHNHPCEELMQTLTPLLHQLQFLSKF 108

[Close]

BLAST of Carg15462 vs. ExPASy Swiss-Prot
Match: Q8VWQ4 (Probable WRKY transcription factor 56 OS=Arabidopsis thaliana OX=3702 GN=WRKY56 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.7e-27
Identity = 63/121 (52.07%), Postives = 80/121 (66.12%), Query Frame = 0


Query: 63  GGAETTEMAAARQGDND--------KKKKTRS-RRFAFQTRSQVDILDDGYRWRKYGQKG 122
           GG E   + +    ++D        K K+T + +R AF TRS  D+LDDGYRWRKYGQK 
Sbjct: 67  GGGEMGGLVSNNSNNSDHNKNCNKGKGKRTLAMQRIAFHTRSDDDVLDDGYRWRKYGQKS 126

Query: 123 VKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQM 175
           VKNN  PRSYY+CT+  C VKKQVQRL +D  VVVTTYEG+H+HP +K  +    +L Q+
Sbjct: 127 VKNNAHPRSYYRCTYHTCNVKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLSPLLRQL 186

[Close]

BLAST of Carg15462 vs. ExPASy TrEMBL
Match: A0A6J1F2W9 (probable WRKY transcription factor 75 OS=Cucurbita moschata OX=3662 GN=LOC111439177 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 5.7e-95
Identity = 178/179 (99.44%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MLISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSKGSAVEV 60
           MLISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSKGSAVEV
Sbjct: 23  MLISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSKGSAVEV 82

Query: 61  VEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP 120
           VEGGAETTEM AARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP
Sbjct: 83  VEGGAETTEMTAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP 142

Query: 121 RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 180
           RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY
Sbjct: 143 RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 201

[Close]

BLAST of Carg15462 vs. ExPASy TrEMBL
Match: A0A345BTD5 (WRKY1 OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.2e-92
Identity = 175/179 (97.77%), Postives = 175/179 (97.77%), Query Frame = 0


Query: 1   MLISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSKGSAVEV 60
           MLISRASEPTGIHGS FREA ISGGFPLFPDVSGSYMSVGTRSS KEDMVSVSKGSAVEV
Sbjct: 23  MLISRASEPTGIHGSQFREAAISGGFPLFPDVSGSYMSVGTRSSTKEDMVSVSKGSAVEV 82

Query: 61  VEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP 120
           VEGGAETTEM AARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP
Sbjct: 83  VEGGAETTEMTAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP 142

Query: 121 RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 180
           RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY
Sbjct: 143 RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 201

[Close]

BLAST of Carg15462 vs. ExPASy TrEMBL
Match: A0A6J1I419 (probable WRKY transcription factor 75 OS=Cucurbita maxima OX=3661 GN=LOC111470780 PE=4 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.9e-90
Identity = 175/183 (95.63%), Postives = 175/183 (95.63%), Query Frame = 0


Query: 2   LISRASEPTGIHGSHFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSK-----GS 61
           LISRASEPTGIHGSHFREAGISGGFPLFPDVS SYMSV TRSSMKEDMVSVSK     GS
Sbjct: 24  LISRASEPTGIHGSHFREAGISGGFPLFPDVSRSYMSVDTRSSMKEDMVSVSKGFHADGS 83

Query: 62  AVEVVEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKN 121
           A EVVEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKN
Sbjct: 84  AGEVVEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKN 143

Query: 122 NKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIY 180
           NKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIY
Sbjct: 144 NKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIY 203

[Close]

BLAST of Carg15462 vs. ExPASy TrEMBL
Match: A0A6J1CVL3 (probable WRKY transcription factor 75 OS=Momordica charantia OX=3673 GN=LOC111014691 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 8.1e-57
Identity = 127/193 (65.80%), Postives = 142/193 (73.58%), Query Frame = 0


Query: 1   MLISRASEPTGIHGS-HFREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVSVSK----- 60
           MLISRA E  GIHG+   +    SGGFP+F DVS   MSV    SMK+D+VS+S+     
Sbjct: 16  MLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRSMKDDVVSLSRSVRFV 75

Query: 61  -GSAVEVVE-------GGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRW 120
            GSA E VE       G   +T       G  D +KK R +R+AFQTRSQVDILDDGYRW
Sbjct: 76  DGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAFQTRSQVDILDDGYRW 135

Query: 121 RKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNF 180
           RKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLT+DE VV+TTYEGIHSHPI KSTDNF
Sbjct: 136 RKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTYEGIHSHPIDKSTDNF 195

[Close]

BLAST of Carg15462 vs. ExPASy TrEMBL
Match: A0A5D3D1A5 (Putative WRKY transcription factor 48 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001590 PE=4 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.7e-54
Identity = 129/200 (64.50%), Postives = 142/200 (71.00%), Query Frame = 0


Query: 1   MLISRASEPTGIHGSHFREAGISGGFP-LFPDVSGSYMSVGTRSSMKED----------- 60
           +L SRAS+  G H  H     +S GFP LF DVS   MS    SS+K++           
Sbjct: 23  ILFSRASDHRGHHFRH--PPSVSDGFPMLFRDVSTDDMSFDATSSVKDEDNNNNNNNNNV 82

Query: 61  MVSVSK------GSAVEVVEGGAET---TEMAAARQGDNDKKKKTRSRRFAFQTRSQVDI 120
           +VSVS+       SA++  E   E      M     GD DKKKK RSRRFAFQTRSQVDI
Sbjct: 83  VVSVSERFRVDGSSALKKAEEEGEVRAGAMMGERGGGDCDKKKKMRSRRFAFQTRSQVDI 142

Query: 121 LDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPI 180
           LDDGYRWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPI
Sbjct: 143 LDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPI 202

[Close]

BLAST of Carg15462 vs. TAIR 10
Match: AT5G13080.1 (WRKY DNA-binding protein 75 )

HSP 1 Score: 174.9 bits (442), Expect = 5.9e-44
Identity = 87/138 (63.04%), Postives = 109/138 (78.99%), Query Frame = 0


Query: 44  SMKEDMVSVSKGSAVEVVEGGAETTEMAA--ARQGDNDKKKKTRSRRFAFQTRSQVDILD 103
           S+    +S+   S  E+ +G  E++++ +    +     KKK + +R+AFQTRSQVDILD
Sbjct: 8   SLYAPFLSLKSHSKPELHQGEEESSKVRSEGCSKSVESSKKKGKKQRYAFQTRSQVDILD 67

Query: 104 DGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQK 163
           DGYRWRKYGQK VKNNKFPRSYY+CT+ GC VKKQVQRLT D+ VVVTTYEG+HSHPI+K
Sbjct: 68  DGYRWRKYGQKAVKNNKFPRSYYRCTYGGCNVKKQVQRLTVDQEVVVTTYEGVHSHPIEK 127

Query: 164 STDNFEHILTQMQIYTSY 180
           ST+NFEHILTQMQIY+S+
Sbjct: 128 STENFEHILTQMQIYSSF 145

[Close]

BLAST of Carg15462 vs. TAIR 10
Match: AT3G01970.1 (WRKY DNA-binding protein 45 )

HSP 1 Score: 159.8 bits (403), Expect = 2.0e-39
Identity = 77/119 (64.71%), Postives = 93/119 (78.15%), Query Frame = 0


Query: 61  VEGGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFP 120
           V+  A+ T  ++  +  + KKKK R  R+AFQTRSQVDILDDGYRWRKYGQK VKNN FP
Sbjct: 26  VDNSAQPT-TSSEEKPRSKKKKKEREARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFP 85

Query: 121 RSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 180
           RSYYKCT +GC+VKKQVQR   DEGVVVTTY+G+H+H + K +DNF HILTQM I+  +
Sbjct: 86  RSYYKCTEEGCRVKKQVQRQWGDEGVVVTTYQGVHTHAVDKPSDNFHHILTQMHIFPPF 143

[Close]

BLAST of Carg15462 vs. TAIR 10
Match: AT5G41570.1 (WRKY DNA-binding protein 24 )

HSP 1 Score: 126.7 bits (317), Expect = 1.9e-29
Identity = 60/106 (56.60%), Postives = 77/106 (72.64%), Query Frame = 0


Query: 75  QGDNDKKKKTRS-RRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKV 134
           +G   K+K++R   R AF TRS  D+LDDGYRWRKYGQK VK+N  PRSYY+CT+  C V
Sbjct: 71  KGKELKEKRSRKVPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTCNV 130

Query: 135 KKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 180
           KKQVQRL +D  VVVTTYEG+H+HP +K  +    +L Q+Q  +S+
Sbjct: 131 KKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLNPLLRQLQFLSSF 176

[Close]

BLAST of Carg15462 vs. TAIR 10
Match: AT2G46130.1 (WRKY DNA-binding protein 43 )

HSP 1 Score: 125.2 bits (313), Expect = 5.4e-29
Identity = 55/103 (53.40%), Postives = 76/103 (73.79%), Query Frame = 0


Query: 77  DNDKKKKTRSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQ 136
           D+ + KK ++ RF+F+T+S  DILDDGYRWRKYGQK VKN+ +PRSYY+CT   C VKKQ
Sbjct: 6   DSSRDKKMKNPRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQ 65

Query: 137 VQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 180
           VQRL+++  +V TTYEGIH+HP ++       +L Q+Q  + +
Sbjct: 66  VQRLSKETSIVETTYEGIHNHPCEELMQTLTPLLHQLQFLSKF 108

[Close]

BLAST of Carg15462 vs. TAIR 10
Match: AT1G64000.1 (WRKY DNA-binding protein 56 )

HSP 1 Score: 124.0 bits (310), Expect = 1.2e-28
Identity = 63/121 (52.07%), Postives = 80/121 (66.12%), Query Frame = 0


Query: 63  GGAETTEMAAARQGDND--------KKKKTRS-RRFAFQTRSQVDILDDGYRWRKYGQKG 122
           GG E   + +    ++D        K K+T + +R AF TRS  D+LDDGYRWRKYGQK 
Sbjct: 67  GGGEMGGLVSNNSNNSDHNKNCNKGKGKRTLAMQRIAFHTRSDDDVLDDGYRWRKYGQKS 126

Query: 123 VKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIQKSTDNFEHILTQM 175
           VKNN  PRSYY+CT+  C VKKQVQRL +D  VVVTTYEG+H+HP +K  +    +L Q+
Sbjct: 127 VKNNAHPRSYYRCTYHTCNVKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLSPLLRQL 186

The following BLAST results are available for this feature:

BLAST of Carg15462 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029102.1	4.1e-95	100.00	putative WRKY transcription factor 75, partial [Cucurbita argyrosperma subsp. ar...	[more]
XP_022932710.1	1.2e-94	99.44	probable WRKY transcription factor 75 [Cucurbita moschata]	[more]
XP_023539084.1	2.5e-92	97.77	probable WRKY transcription factor 75 isoform X1 [Cucurbita pepo subsp. pepo] >A...	[more]
XP_022972157.1	3.9e-90	95.63	probable WRKY transcription factor 75 [Cucurbita maxima]	[more]
XP_038902977.1	2.9e-61	69.74	probable WRKY transcription factor 50 [Benincasa hispida]	[more]

BLAST of Carg15462 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYA2	8.4e-43	63.04	Probable WRKY transcription factor 75 OS=Arabidopsis thaliana OX=3702 GN=WRKY75 ...	[more]
Q9S763	2.8e-38	64.71	Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 ...	[more]
Q9FFS3	2.6e-28	56.60	Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 ...	[more]
Q8GY11	7.6e-28	53.40	Probable WRKY transcription factor 43 OS=Arabidopsis thaliana OX=3702 GN=WRKY43 ...	[more]
Q8VWQ4	1.7e-27	52.07	Probable WRKY transcription factor 56 OS=Arabidopsis thaliana OX=3702 GN=WRKY56 ...	[more]

BLAST of Carg15462 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F2W9	5.7e-95	99.44	probable WRKY transcription factor 75 OS=Cucurbita moschata OX=3662 GN=LOC111439...	[more]
A0A345BTD5	1.2e-92	97.77	WRKY1 OS=Cucurbita pepo OX=3663 PE=2 SV=1	[more]
A0A6J1I419	1.9e-90	95.63	probable WRKY transcription factor 75 OS=Cucurbita maxima OX=3661 GN=LOC11147078...	[more]
A0A6J1CVL3	8.1e-57	65.80	probable WRKY transcription factor 75 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A5D3D1A5	1.7e-54	64.50	Putative WRKY transcription factor 48 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]

BLAST of Carg15462 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G13080.1	5.9e-44	63.04	WRKY DNA-binding protein 75	[more]
AT3G01970.1	2.0e-39	64.71	WRKY DNA-binding protein 45	[more]
AT5G41570.1	1.9e-29	56.60	WRKY DNA-binding protein 24	[more]
AT2G46130.1	5.4e-29	53.40	WRKY DNA-binding protein 43	[more]
AT1G64000.1	1.2e-28	52.07	WRKY DNA-binding protein 56	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 100..159 e-value: 1.4E-36 score: 137.6
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 101..158 e-value: 3.7E-25 score: 88.0
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 95..160 score: 29.401716
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 84..159 e-value: 2.9E-33 score: 115.5
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 92..159
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR31221	WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED	coord: 57..176
None	No IPR available	PANTHER	PTHR31221:SF83	WRKY TRANSCRIPTION FACTOR 75-RELATED	coord: 57..176

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg15462-RA	Carg15462-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding