Carg15389 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg15389
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DUF1296 domain-containing protein
Location	Carg_Chr03: 9546198 .. 9548342 (+)
RNA-Seq Expression	Carg15389
Synteny	Carg15389

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGGAGACTAGAGAGAGAGCGAGAGAGAGAGAGGGAAAGGGTTTTCGATGCGTAACAAAAAGCAGAACGAGAGACGAGAGAGAGAATCGTATACAGTGCATCGACAAACATATCTTCTTCCTGTCACGGCGGAACCCTAGCGGGAGACGGACTAAAGCCTGTCCTTTTGAAAGGTTTCTCAGATCTGAACCCCCGGCCGGCGAGGGAGAGAGGTATCCAAACATAGTGAAACAGTATGGGTGCCAAAGGAGGAGGAGGAGGAGGAAGCAAAAGCACCAATGGAGCATCTGGAATTCCGGCCGAGTGGCGGAAGATGGTCCAGAGCTTGAAAGAGATTGTAAATAACTGTACCGACCAGGAGATCTATGCTACCCTAAGAGAATGTAATATGGATCCTGATGAAGCCGTTAATCGCCTCATTACGCAAGGTCTTTTCTTTTTTCTCCTATTTCCACGTTCACGGTTCTCTATTTTTCTTGCGTCGGAAAGTTTAATCATCCTTGTGTTTTACCCGCCGGGTTTTGCCGCGAGAACTCTTGGTCGTGAAATCCAAGTTCGTACTTGATTATGCAAATGTTTTGTTTAAATGTTGAAGTTGAAGGTTGTATTTTCACTGTCATCGTTGGTGAAGTGAGACCAGAAAGAATAATCTTGCCAACTTCTGTTCGTGCTTCTGTCAACCCATACGAGACATTTATGCTTTTGGTGCTAACAAGTACATGTTTTTAGAATGAGTATCTTCTAGGACATTTGTGGTTGTGCGTTCTGTTAGTTTCTCTCTGTGCGACTCTAAAGGTTTAAAGATTATAATCATTTTGTTCTATCGTTGTGTAGACCCTTTTCATGAGGTGAAGAGCAAACGAGAAAAGAAAAAAGAGGTGCGTATGTGAGAGGAATGCAGATGTTTGTTCTAAAAGCTTTTACCATGTCATTCAGATACTGCATTCATGTTGGCTTTAGTAGATATCTTATCTCATACACATATAATTGATCTTGACTTATTTTCTCAATGTTCTTCACTCCTCTCTTTTTTCTTGCTTCTTCAGAATAAGGATCCGGTTGATTCCAGATCTCGTGGGCCTAATATCCCTTCCAGTCGTACGAGTAAGGGTGGTCCAGATCGTTATGCTGGACGCAGTAGCTTGAACCAGTTTGGTTCTAGTGGTATGTGAACGATTCTTTGTAGTTGTAAATTTCATAAGTTTTTCACACATTGTTGATGTACTTATTTATTTATTTTTTGTTAGAAACTGGTGTGTTGCACAGTAAACCTGTGTACAAAAAGGAAAATGGAGCTAATGATCACGCTGGTTCGTCTTCTGCATCTGGTCAATCCGGAAATCACTCATACCACCAATTTCCATCTCACAGGTTAAATGTTGTAAGATTACGTTGTACTTGTCAATTGTGAAGTACTGACTACTATGCAATGCAATTCCATGGTTGAACATTATTTTTGGATACTGGATAATGAACTCTTTGCCAGTTATGTTTCAAATAAGTATATTATTTTTCTCCAAGTACTGGAGCAATGTGAAGTTTCCCTTTGTCGAATTTTTTTCATATGTGATTTTGAATGTAGACAAGTGGGAAAATTTTTTAAATTTGCGTCTGAATTTTAGTTTACAAAATTTCTCCAATTATTTTTGAATTTTAGTTTACAAAATTTTTTTCATACTTCATTTGCGTCTGAATTTTTTATTTTTTTATTTTTTTATTTTTTTACATTGTGGTATGCTTCATCTAGGTTATTGGTAGCAAATGAAGTATGAAATTTGTCTTGAAATAGTTGGAAAGCATTTCCTGTCCTTTATATGACTAAATATAGACATCAAAGCTGTATCATTCATTAAAGGGTAAGAATTGTGGTGTTTTAGGCTGAATACTCCTTTACTTAAGTATTTTAAGTTTCTGGTTTTTGGATTGTTCATATGATCTTGAAAAATATTTGTTACATTGCTAACTTGTCTCCATCAATTGTCTTAAGGTTTAGATAATTTTGTTGATCTGGTTTCTTTAATACGGAGTTTTTTTTTTCTAATTGCCCCTGCTAAAAAGGTAAGAAGCATAAACATTGACACACGACATGGGCATGACAACATGTCAATTTCCAAGAAAAGGACTCAACACATTGGGACACGTTAA

mRNA sequence

AGGGAGACTAGAGAGAGAGCGAGAGAGAGAGAGGGAAAGGGTTTTCGATGCGTAACAAAAAGCAGAACGAGAGACGAGAGAGAGAATCGTATACAGTGCATCGACAAACATATCTTCTTCCTGTCACGGCGGAACCCTAGCGGGAGACGGACTAAAGCCTGTCCTTTTGAAAGGTTTCTCAGATCTGAACCCCCGGCCGGCGAGGGAGAGAGGTATCCAAACATAGTGAAACAGTATGGGTGCCAAAGGAGGAGGAGGAGGAGGAAGCAAAAGCACCAATGGAGCATCTGGAATTCCGGCCGAGTGGCGGAAGATGGTCCAGAGCTTGAAAGAGATTGTAAATAACTGTACCGACCAGGAGATCTATGCTACCCTAAGAGAATGTAATATGGATCCTGATGAAGCCGTTAATCGCCTCATTACGCAAGGTTGTATTTTCACTGTCATCGTTGGTGAAGTGAGACCAGAAAGAATAATCTTGCCAACTTCTGTTCGTGCTTCTGTCAACCCATACGAGACATTTATGCTTTTGGTGCTAACAAACCCTTTTCATGAGGTGAAGAGCAAACGAGAAAAGAAAAAAGAGAATAAGGATCCGGTTGATTCCAGATCTCGTGGGCCTAATATCCCTTCCAGTCGTACGAGTAAGGGTGGTCCAGATCGTTATGCTGGACGCAGTAGCTTGAACCAGTTTGGTTCTAGTGAAACTGGTGTGTTGCACAGTAAACCTGTGTACAAAAAGGAAAATGGAGCTAATGATCACGCTGGTTCGTCTTCTGCATCTGGTCAATCCGGAAATCACTCATACCACCAATTTCCATCTCACAGGTTATTGGTAGCAAATGAAGTAAGAAGCATAAACATTGACACACGACATGGGCATGACAACATGTCAATTTCCAAGAAAAGGACTCAACACATTGGGACACGTTAA

Coding sequence (CDS)

ATGGGTGCCAAAGGAGGAGGAGGAGGAGGAAGCAAAAGCACCAATGGAGCATCTGGAATTCCGGCCGAGTGGCGGAAGATGGTCCAGAGCTTGAAAGAGATTGTAAATAACTGTACCGACCAGGAGATCTATGCTACCCTAAGAGAATGTAATATGGATCCTGATGAAGCCGTTAATCGCCTCATTACGCAAGGTTGTATTTTCACTGTCATCGTTGGTGAAGTGAGACCAGAAAGAATAATCTTGCCAACTTCTGTTCGTGCTTCTGTCAACCCATACGAGACATTTATGCTTTTGGTGCTAACAAACCCTTTTCATGAGGTGAAGAGCAAACGAGAAAAGAAAAAAGAGAATAAGGATCCGGTTGATTCCAGATCTCGTGGGCCTAATATCCCTTCCAGTCGTACGAGTAAGGGTGGTCCAGATCGTTATGCTGGACGCAGTAGCTTGAACCAGTTTGGTTCTAGTGAAACTGGTGTGTTGCACAGTAAACCTGTGTACAAAAAGGAAAATGGAGCTAATGATCACGCTGGTTCGTCTTCTGCATCTGGTCAATCCGGAAATCACTCATACCACCAATTTCCATCTCACAGGTTATTGGTAGCAAATGAAGTAAGAAGCATAAACATTGACACACGACATGGGCATGACAACATGTCAATTTCCAAGAAAAGGACTCAACACATTGGGACACGTTAA

Protein sequence

MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNRLITQGCIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKDPVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSSSASGQSGNHSYHQFPSHRLLVANEVRSINIDTRHGHDNMSISKKRTQHIGTR

Homology

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BLAST of Carg15389 vs. NCBI nr
Match: KAG7034689.1 (hypothetical protein SDJN02_04419 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 453.8 bits (1166), Expect = 9.2e-124
Identity = 232/232 (100.00%), Postives = 232/232 (100.00%), Query Frame = 0


Query: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60
           MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR
Sbjct: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60

Query: 61  LITQGCIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKD 120
           LITQGCIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKD
Sbjct: 61  LITQGCIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKD 120

Query: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS 180
           PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS
Sbjct: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS 180

Query: 181 SASGQSGNHSYHQFPSHRLLVANEVRSINIDTRHGHDNMSISKKRTQHIGTR 233
           SASGQSGNHSYHQFPSHRLLVANEVRSINIDTRHGHDNMSISKKRTQHIGTR
Sbjct: 181 SASGQSGNHSYHQFPSHRLLVANEVRSINIDTRHGHDNMSISKKRTQHIGTR 232

[Close]

BLAST of Carg15389 vs. NCBI nr
Match: KAG6581405.1 (GBF-interacting protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 293.5 bits (750), Expect = 1.6e-75
Identity = 161/215 (74.88%), Postives = 165/215 (76.74%), Query Frame = 0


Query: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60
           MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR
Sbjct: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60

Query: 61  LITQGCIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKD 120
           LITQ                                      +PFHEVKSKREKKKENKD
Sbjct: 61  LITQ--------------------------------------DPFHEVKSKREKKKENKD 120

Query: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS 180
           PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS
Sbjct: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS 177

Query: 181 SASGQSGNHSYHQFPSHRLLVANEVRSINIDTRHG 216
           SASGQSGNHSYHQFPSH   V  E +   +   +G
Sbjct: 181 SASGQSGNHSYHQFPSHSNNVVTENKLSGLGAGNG 177

[Close]

BLAST of Carg15389 vs. NCBI nr
Match: XP_022925602.1 (uncharacterized protein LOC111432988 [Cucurbita moschata])

HSP 1 Score: 293.1 bits (749), Expect = 2.1e-75
Identity = 160/204 (78.43%), Postives = 161/204 (78.92%), Query Frame = 0


Query: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60
           MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR
Sbjct: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60

Query: 61  LITQGCIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKD 120
           LITQ                                      +PFHEVKSKREKKKENKD
Sbjct: 61  LITQ--------------------------------------DPFHEVKSKREKKKENKD 120

Query: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS 180
           PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS
Sbjct: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS 166

Query: 181 SASGQSGNHSYHQFPSHRLLVANE 205
           SASGQSGNHSYHQFPSH   V  E
Sbjct: 181 SASGQSGNHSYHQFPSHSNNVVTE 166

[Close]

BLAST of Carg15389 vs. NCBI nr
Match: XP_023543650.1 (putative uncharacterized protein DDB_G0277255 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023543651.1 putative uncharacterized protein DDB_G0277255 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 285.8 bits (730), Expect = 3.3e-73
Identity = 159/207 (76.81%), Postives = 160/207 (77.29%), Query Frame = 0


Query: 1   MGAK---GGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEA 60
           MGAK   GGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEA
Sbjct: 1   MGAKGGGGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEA 60

Query: 61  VNRLITQGCIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKE 120
           VNRLITQ                                      +PFHEVKSKREKKKE
Sbjct: 61  VNRLITQ--------------------------------------DPFHEVKSKREKKKE 120

Query: 121 NKDPVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHA 180
           NKDPVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHA
Sbjct: 121 NKDPVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHA 169

Query: 181 GSSSASGQSGNHSYHQFPSHRLLVANE 205
           GSSSASGQ GNHSYHQFPSH   V  E
Sbjct: 181 GSSSASGQPGNHSYHQFPSHSNNVVTE 169

[Close]

BLAST of Carg15389 vs. NCBI nr
Match: XP_022978644.1 (uncharacterized protein LOC111478557 [Cucurbita maxima])

HSP 1 Score: 277.7 bits (709), Expect = 9.1e-71
Identity = 157/215 (73.02%), Postives = 160/215 (74.42%), Query Frame = 0


Query: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60
           MGAKGGGG   KSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR
Sbjct: 1   MGAKGGGG---KSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60

Query: 61  LITQGCIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKD 120
           LITQ                                      +PFHEVKSKREKKKENKD
Sbjct: 61  LITQ--------------------------------------DPFHEVKSKREKKKENKD 120

Query: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS 180
           PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS
Sbjct: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS 174

Query: 181 SASGQSGNHSYHQFPSHRLLVANEVRSINIDTRHG 216
           SASGQSGNHS +QFPSH   V  E +   I    G
Sbjct: 181 SASGQSGNHSNYQFPSHSNNVVTENKLSGIGAGDG 174

[Close]

BLAST of Carg15389 vs. ExPASy TrEMBL
Match: A0A6J1EC50 (uncharacterized protein LOC111432988 OS=Cucurbita moschata OX=3662 GN=LOC111432988 PE=4 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 1.0e-75
Identity = 160/204 (78.43%), Postives = 161/204 (78.92%), Query Frame = 0


Query: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60
           MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR
Sbjct: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60

Query: 61  LITQGCIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKD 120
           LITQ                                      +PFHEVKSKREKKKENKD
Sbjct: 61  LITQ--------------------------------------DPFHEVKSKREKKKENKD 120

Query: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS 180
           PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS
Sbjct: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS 166

Query: 181 SASGQSGNHSYHQFPSHRLLVANE 205
           SASGQSGNHSYHQFPSH   V  E
Sbjct: 181 SASGQSGNHSYHQFPSHSNNVVTE 166

[Close]

BLAST of Carg15389 vs. ExPASy TrEMBL
Match: A0A6J1IUL3 (uncharacterized protein LOC111478557 OS=Cucurbita maxima OX=3661 GN=LOC111478557 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 4.4e-71
Identity = 157/215 (73.02%), Postives = 160/215 (74.42%), Query Frame = 0


Query: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60
           MGAKGGGG   KSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR
Sbjct: 1   MGAKGGGG---KSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60

Query: 61  LITQGCIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKD 120
           LITQ                                      +PFHEVKSKREKKKENKD
Sbjct: 61  LITQ--------------------------------------DPFHEVKSKREKKKENKD 120

Query: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS 180
           PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS
Sbjct: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS 174

Query: 181 SASGQSGNHSYHQFPSHRLLVANEVRSINIDTRHG 216
           SASGQSGNHS +QFPSH   V  E +   I    G
Sbjct: 181 SASGQSGNHSNYQFPSHSNNVVTENKLSGIGAGDG 174

[Close]

BLAST of Carg15389 vs. ExPASy TrEMBL
Match: A0A6J1CL91 (uncharacterized protein LOC111012321 OS=Momordica charantia OX=3673 GN=LOC111012321 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 7.2e-66
Identity = 144/204 (70.59%), Postives = 153/204 (75.00%), Query Frame = 0


Query: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60
           MGA+GGGGG  K+ NG SGIPAEWRKMVQSLKEIVNNCTDQEIYATLR+CNMDPDEAVNR
Sbjct: 1   MGARGGGGG--KTNNGTSGIPAEWRKMVQSLKEIVNNCTDQEIYATLRDCNMDPDEAVNR 60

Query: 61  LITQGCIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKD 120
           L+TQ                                      +PFHEVKSKREKKKENKD
Sbjct: 61  LLTQ--------------------------------------DPFHEVKSKREKKKENKD 120

Query: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSS 180
           P+DSRSRGPNIPSSR SKGG DRYAGR SLNQFGSSE+GVLHSKPVYKKENGAND AGSS
Sbjct: 121 PIDSRSRGPNIPSSRPSKGGTDRYAGRGSLNQFGSSESGVLHSKPVYKKENGANDQAGSS 164

Query: 181 SASGQSGNHSYHQFPSHRLLVANE 205
           SASGQSGN +Y+QFPSH   VA E
Sbjct: 181 SASGQSGNQTYYQFPSHSNNVATE 164

[Close]

BLAST of Carg15389 vs. ExPASy TrEMBL
Match: A0A5D3CS90 (DUF1296 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008740 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 3.0e-64
Identity = 148/227 (65.20%), Postives = 168/227 (74.01%), Query Frame = 0


Query: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60
           MGAKGGGGG  K+TNG SGIP EWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR
Sbjct: 1   MGAKGGGGG--KTTNGTSGIPPEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60

Query: 61  LITQGCIFTVI-VGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENK 120
           L+TQG  F              +P    +         LL L +PFHEVKSKREKKKENK
Sbjct: 61  LLTQGLSFLFFSFFFFXXXXXFIPCRWFSLCLSVSLSFLLRLEDPFHEVKSKREKKKENK 120

Query: 121 DPVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGS 180
           DP+DSRSRG +IPSSRTSK G +RYAGRSS  QFGSS+TG+  SKPVYKKENGA+DHAGS
Sbjct: 121 DPIDSRSRGSSIPSSRTSKSGTERYAGRSSSIQFGSSDTGLSPSKPVYKKENGASDHAGS 180

Query: 181 SSASGQSGNHSYHQFPSHRLLVANEVRSINIDTRHGHDNMSISKKRT 227
           SS SGQSGNHS++Q+PSH    +N V + N  +  G  + +IS  +T
Sbjct: 181 SSVSGQSGNHSFYQYPSHS---SNNVATENKLSGLGAGDGAISSSQT 222

[Close]

BLAST of Carg15389 vs. ExPASy TrEMBL
Match: A0A0A0KNR5 (DUF1296 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G522110 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.4e-61
Identity = 140/219 (63.93%), Postives = 156/219 (71.23%), Query Frame = 0


Query: 8   GGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNRLITQGCI 67
           GGG K+TNG SGIP EWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNRL+TQ   
Sbjct: 64  GGGGKTTNGTSGIPPEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNRLLTQ--- 123

Query: 68  FTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKDPVDSRSR 127
                                              +PFHEVKSKREKKKENKDP+DSRSR
Sbjct: 124 -----------------------------------DPFHEVKSKREKKKENKDPIDSRSR 183

Query: 128 GPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSSSASGQSG 187
           G +IPSSRTSKGG DRYAGRSS  QFGSS+TG+  SKPVYKKENGA+DHAGSSSASGQSG
Sbjct: 184 GSSIPSSRTSKGGTDRYAGRSSSIQFGSSDTGLSSSKPVYKKENGASDHAGSSSASGQSG 241

Query: 188 NHSYHQFPSHRLLVANEVRSINIDTRHGHDNMSISKKRT 227
           NHS++QFPSH    +N V + N  +  G  + +IS  +T
Sbjct: 244 NHSFYQFPSHS---SNNVATENKLSGLGAGDGAISSSQT 241

[Close]

BLAST of Carg15389 vs. TAIR 10
Match: AT4G18150.1 (Kinase-related protein of unknown function (DUF1296) )

HSP 1 Score: 109.4 bits (272), Expect = 4.0e-24
Identity = 81/184 (44.02%), Postives = 105/184 (57.07%), Query Frame = 0


Query: 6   GGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNRLITQG 65
           GGGGGS S N   G+PA  RK++Q LKEIV  C++ EIYA L EC+M+PDEAVNRL++Q 
Sbjct: 2   GGGGGSVS-NANGGVPASSRKVIQDLKEIV-ECSELEIYAMLVECDMNPDEAVNRLLSQ- 61

Query: 66  CIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKDPVDSR 125
                                                + FHEVKSKREKKKE KD  DS 
Sbjct: 62  -------------------------------------DTFHEVKSKREKKKETKDHADSW 121

Query: 126 SRGPNIPSSRTSKGGPDRY-AGRSSLNQFGSSETGVLHSKPVYKKENGANDH-AGSSSAS 185
           +R  N+P +R ++ G D Y   R   N++ S+ETG +   PV ++ENGA +H AGSS++S
Sbjct: 122 TR--NVP-NRGARSGNDSYNTSRGGRNKYKSNETGKIQGVPVNRRENGARNHLAGSSASS 142

Query: 186 GQSG 188
           G  G
Sbjct: 182 GLLG 142

[Close]

BLAST of Carg15389 vs. TAIR 10
Match: AT1G29350.1 (Kinase-related protein of unknown function (DUF1296) )

HSP 1 Score: 104.8 bits (260), Expect = 9.8e-23
Identity = 85/229 (37.12%), Postives = 117/229 (51.09%), Query Frame = 0


Query: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60
           M +   GGG  K   G   IP+  RK+VQSL EIVN+  + EIYA L+ECNMDP+E V+R
Sbjct: 1   MSSSKVGGGARK---GIQDIPSGSRKIVQSLTEIVNS-PEAEIYAMLKECNMDPNETVSR 60

Query: 61  LITQGCIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKD 120
           L++Q                                      +PFHEVKSK+EKKKE +D
Sbjct: 61  LLSQ--------------------------------------DPFHEVKSKKEKKKETRD 120

Query: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGS- 180
             DSR RG N   +R ++GG  RYAGRS    F S+++G    K   KKE+G   +  S 
Sbjct: 121 ISDSRPRGANNTYNRGARGGSARYAGRSGSTHFSSTDSGNFQGKSTNKKESGTQGYTSSW 180

Query: 181 SSASGQSGNHSYHQFPSHRLLVANEVRSINIDTRHGHDNMSISKKRTQH 229
           SSASG +  ++Y    S  + + N++  + +      D +S SK  + H
Sbjct: 181 SSASGVA--NTYQTPHSEPIAMENKLPPVTLG-----DGISSSKSASGH 180

[Close]

BLAST of Carg15389 vs. TAIR 10
Match: AT1G29370.1 (Kinase-related protein of unknown function (DUF1296) )

HSP 1 Score: 102.4 bits (254), Expect = 4.9e-22
Identity = 84/229 (36.68%), Postives = 116/229 (50.66%), Query Frame = 0


Query: 1   MGAKGGGGGGSKSTNGASGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNR 60
           M +   GGG  K   G   IP+  RK+VQSL EIVN+  + EIYA L+ECNMDP+E V+R
Sbjct: 1   MSSSKVGGGARK---GIQDIPSGSRKIVQSLTEIVNS-PEAEIYAMLKECNMDPNETVSR 60

Query: 61  LITQGCIFTVIVGEVRPERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKD 120
           L++Q                                      +PFHEVKSK+EKKKE +D
Sbjct: 61  LLSQ--------------------------------------DPFHEVKSKKEKKKETRD 120

Query: 121 PVDSRSRGPNIPSSRTSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGS- 180
             DSR RG N   +R ++GG  RYAGRS    F S+++G    K   KKE+G   +  S 
Sbjct: 121 ISDSRPRGANNTYNRGARGGSARYAGRSGSTHFSSTDSGNFQGKSTNKKESGTQGYTSSW 180

Query: 181 SSASGQSGNHSYHQFPSHRLLVANEVRSINIDTRHGHDNMSISKKRTQH 229
           SSASG +  ++Y    S  +   N++  + +      D +S S+  + H
Sbjct: 181 SSASGVA--NTYQTPHSEPVATENKLPPVTLG-----DGISSSQSASGH 180

[Close]

BLAST of Carg15389 vs. TAIR 10
Match: AT5G46380.1 (Kinase-related protein of unknown function (DUF1296) )

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-10
Identity = 52/140 (37.14%), Postives = 70/140 (50.00%), Query Frame = 0


Query: 18  SGIPAEWRKMVQSLKEIVNNCTDQEIYATLRECNMDPDEAVNRLITQGCIFTVIVGEVRP 77
           + IP   R +V+S+KEIV NC++QEIYA L ECNM+ DEA+ RL++Q             
Sbjct: 9   NAIPEYTRAIVRSMKEIV-NCSEQEIYAMLVECNMNADEAITRLLSQ------------- 68

Query: 78  ERIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKENKDPVD-SRSRGPNIPSSRT 137
                                    + F EVKSKR+KKKE KD ++  R  G N      
Sbjct: 69  -------------------------DSFQEVKSKRDKKKEAKDALEIQRLSGRN--QYHK 106

Query: 138 SKGGPDRYAGRSSLNQFGSS 157
           SK G D+  G + LN +G+S
Sbjct: 129 SKNGSDQNGG-NKLNSYGTS 106

[Close]

BLAST of Carg15389 vs. TAIR 10
Match: AT3G07660.1 (Kinase-related protein of unknown function (DUF1296) )

HSP 1 Score: 63.9 bits (154), Expect = 1.9e-10
Identity = 55/170 (32.35%), Postives = 75/170 (44.12%), Query Frame = 0


Query: 20  IPAEWRKMVQSLKE-IVNNCTDQEIYATLRECNMDPDEAVNRLITQGCIFTVIVGEVRPE 79
           I A  RKM+Q++KE    N ++ EI A L ECNMDPDE   RL+ Q              
Sbjct: 10  ISATTRKMIQNIKETTAGNYSEDEIIAMLHECNMDPDETAQRLLLQ-------------- 69

Query: 80  RIILPTSVRASVNPYETFMLLVLTNPFHEVKSKREKKKE---NKDPVDSRSRGPNIPSSR 139
                                   +PFHEVK KR+K+KE   NKD  +S+ R       R
Sbjct: 70  ------------------------DPFHEVKKKRDKRKENINNKDSAESQWRSGG--GGR 129

Query: 140 TSKGGPDRYAGRSSLNQFGSSETGVLHSKPVYKKENGANDHAGSSSASGQ 186
            S+GG   ++ R + N        V  +K  +KKENG       S+++ Q
Sbjct: 130 GSRGGRMNFSSRHASND-------VAGAKNSFKKENGPKQVIDPSTSTSQ 132

The following BLAST results are available for this feature:

BLAST of Carg15389 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7034689.1	9.2e-124	100.00	hypothetical protein SDJN02_04419 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6581405.1	1.6e-75	74.88	GBF-interacting protein 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022925602.1	2.1e-75	78.43	uncharacterized protein LOC111432988 [Cucurbita moschata]	[more]
XP_023543650.1	3.3e-73	76.81	putative uncharacterized protein DDB_G0277255 isoform X1 [Cucurbita pepo subsp. ...	[more]
XP_022978644.1	9.1e-71	73.02	uncharacterized protein LOC111478557 [Cucurbita maxima]	[more]

BLAST of Carg15389 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg15389 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EC50	1.0e-75	78.43	uncharacterized protein LOC111432988 OS=Cucurbita moschata OX=3662 GN=LOC1114329...	[more]
A0A6J1IUL3	4.4e-71	73.02	uncharacterized protein LOC111478557 OS=Cucurbita maxima OX=3661 GN=LOC111478557...	[more]
A0A6J1CL91	7.2e-66	70.59	uncharacterized protein LOC111012321 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A5D3CS90	3.0e-64	65.20	DUF1296 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A0A0KNR5	1.4e-61	63.93	DUF1296 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G522110 PE=...	[more]

BLAST of Carg15389 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18150.1	4.0e-24	44.02	Kinase-related protein of unknown function (DUF1296)	[more]
AT1G29350.1	9.8e-23	37.12	Kinase-related protein of unknown function (DUF1296)	[more]
AT1G29370.1	4.9e-22	36.68	Kinase-related protein of unknown function (DUF1296)	[more]
AT5G46380.1	1.1e-10	37.14	Kinase-related protein of unknown function (DUF1296)	[more]
AT3G07660.1	1.9e-10	32.35	Kinase-related protein of unknown function (DUF1296)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009719	GBF-interacting protein 1, N-terminal	PFAM	PF06972	DUF1296	coord: 81..117 e-value: 5.8E-7 score: 29.5 coord: 20..67 e-value: 1.0E-21 score: 76.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 211..232
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 174..192
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 108..192
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 108..124
None	No IPR available	PANTHER	PTHR46445:SF3	RNA POLYMERASE II DEGRADATION FACTOR-LIKE PROTEIN (DUF1296)	coord: 1..65
None	No IPR available	PANTHER	PTHR46445:SF3	RNA POLYMERASE II DEGRADATION FACTOR-LIKE PROTEIN (DUF1296)	coord: 91..220
None	No IPR available	PANTHER	PTHR46445	RNA POLYMERASE II DEGRADATION FACTOR-LIKE PROTEIN (DUF1296)	coord: 91..220 coord: 1..65
IPR009060	UBA-like superfamily	SUPERFAMILY	46934	UBA-like	coord: 18..64

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg15389-RA	Carg15389-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0005515	protein binding