Carg15263 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15263
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptiondiacylglycerol kinase
LocationCarg_Chr10: 1964558 .. 1968415 (-)
RNA-Seq ExpressionCarg15263
SyntenyCarg15263
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCATTCCAGAGACGGTGGCGACCAGGTTAGTATAGCAGCTCGCTCCTCGTTATTCGACTCACTGAAAGGATGCGGCCTGTCGGGGATCCCAATCGACAAACAGGAGCTCAATACAAAGGTTATGATGCCGCAGTATCTCCGCTTGGCCTTGCGTGATTCTATCCGCTTAAAAGACCCTACTACAGGAGCCTCTGCCTCTGTTCCACCACAAAATATAGTCCCGCCACTGCCAGAGGCTCCTTTGGTGGTCTTCATTAATCCGCGTAGCGGCGGCCGCCATGGCCAGAAACTCAAGCAGCGCCTCCAGCAATTAATCAGCGAAGAACAGGTCCACCCAAAAAATGCTTTCCACTTTCTGTGAAAAACATCGAGATTCCCATACGTTTTCTTCTTTTCATGTGTGTCCTGTACGTGTCATCTTTGGAATTTTCTCTTTGTTTGTGTTGTAGGTTTTTGATCTTTTGGAAGTGAAGCCTCATGAATTTGTACAATATGGACTGCGCTGCCTGGAAATCTTGGCTGATCAAGGTGACATCTGTGCTAAACAAACTCGTCAGAGGATGAGGGTCATGGTATTTTGATATTTCCAATAACCCCAGATAAAAAGATTGTTGCTTTTCTTTTGCTTTACATATTTGGAATCTAATGGAACTGGTAGCGCAGGTTGCAGGAGGCGATGGTACTGTCGGTTGGGTACTGGGAAGTCTAGCAGAACTTTACAAACAAGACCGCAAGCCTGTTCTTCCTGTTGGGATTATCCCTCTTGGTACTGGCAATGATCTTTCCAGGAGTTTTGGTTGGGTAAGCTTTTGTAGTTCATCTCGAAATTTTGCAGGAAGGTATCACTCGTATTAATGGATATGGATCATTGGTTTTTATCAGGGAGGTTCATTTCCTTTTGCCTGGCAATCAGCTGTTAAAAGGTCTCTCCACAGGGCTACCACTGGCCAAGTTGGTTATTTGGATAGGTGGGAATTCGAGTTCTTTCATGATATTACCTTTTGTTTTTGGTTTTTGGAGCTGTTTCAAACTTGATTCTCATGATGTAGTTGGCATGTTTTGCTGTCGGTGCCTGCTGGGGAGAAAGTGGAACCACCTCATTCTTTGAAGCTTACAGAAGAAAGTGATCTGAATGAGGCTGTGCGTGTATATACTCTTTCGCTCTAAAATTCTAGATATACATAGAGTTTGAACATTGCCCATCTTGGATCATATTTTCATTCTCATTTCAATTCATTATACCCCTTCCATGGCTGCAGAAACTGGACGAAAAAGGAGATTTACCAACAAAACTGGCTTCCTATGAAGGAGTATTTTATAATTACTTCAGCATAGGTAAGCTGAACTCACTGGTGAAGTAAAACTTTAAAAAAGTGTGTTTATTGCACTGAATTTCTTGGTTCAACAAAGATGAGTGTGTCTTGAACTGATCGAAAACGGTCCAAGCTTATCGCTAGCCGATATGGACTTTGAGCTTTCCCTTCTGGGCTTCCCCTCAAGGGGGGTTTTAAACTGCACTCTTGCAGGGCTCCTCCACACCCTTATAAAATTTTTGCGTTCCCCTCTCCAATGGGTTTGAGCCACTACAAATGGTATCAGAGTCAGTCACCGGATAGTGAGAGGCACTGGTCGGAGTAGTGCTAGACTCTCTCCATAGTAGACACGTTATAAAACGTCGAGGGGTAGCTCGGAAAGAAAAGCTCAAAGAGGACAATATCTGCTAGCGGTGGACTTGGGCTAATAGAGTTGCTCCATGGAGAATTAGATTGATATTCAATCATTCAGCTCTAATTCTTTAAGAACTAATCTACTTGCTTCTTCAGAAAAATCATTAGCAGGCTCTGTAGAGTATTTCAGAGGCTATTTAACTGATTCAGTGAATGCATAACGCAGGAATGGATGCTCAAGTTGCTTACGGCTTCCATCATTTGCGTAACAAAAGACCATACCTTGCTCAAGGTCCACTTACAAATAAGGTTGAACTTACCTGCTTAGACCTTAAACAAAAATATTATTTCACGATTAGTGGATGTTTACCAACTTACACGTGCATTCTTTTATATGTGAAAGCTGATCTACTCTAGTTATAGTTGCACTCAAGGTTGGTTCTTCACTTCCTGCTCAAACGATCCAAATTTGAGGTGATACTTTATATCCAATAAAATCCCAGTAGAACACATGTTTTTGCAGTTTCTAAGAACAAAAAGCCTCTCTGTTTGTAGGGGCCTTAAGAACATCGTTAGAATACATATCAAAAAGATAAATTGCTCAGAATGGGAAAAGATTCCAATTCCTTCCAGGTAGAACTGTTCTTGGTGAAACTTGTATAGTCTGTCACTCTTACTGATCCTTTGTTGCTTGGGCTGTTGTGTAGTGTTAGGTCTATTGTTACTTTAAACCTTCATAACTATGCAAGCGGAAGAAACCCATGGGGTAAGCCCAAGCCACAGTACTTGGAGAAGGTACCCACTATGAAGCTCTGTGTGGCTGTTTTCTCTTGTCTATGCTATTATGCTTTATTGACTTCTGATCTTTATCTGGTGCTAGAGAGGCTTTGTGGAGGCGCATGCTGATGATGGTCTTCTAGAGATTTTTGGCCTTAAACAAGGATGGCATACATCGTTCGTTATGGTAGACATAATCTCCGCCAAACACATCGCCCAGGTACTTCAAACACCTAAGAATCACGAAGTCCACTTTGAGCATAAGCTCTCGTGGTTTTGCCTCATACCAATATAGATCTATTCCTTACTCATAAATTTACGATCTAACGCCTTAGTTAGCCGACGTGGGGACTCATCTCCCAACGGTAACAACTCGACTTAAATAGCCACCATGATTCTTGCTTCTGAAGTGTTGCATACTATTTTTCAGGCAGCAGCCATTAGAATAGAAATCAGAGGTGGGAAGCGGAAGAAGGCATATCTGCAGATGGATGGGGAGCCATGGAAACAGCCAATCAATAAGGACTTCTCAACGTATGTTGAAATCAAGAGGGTTCCTTTTCAATCACTAATGATCAACGGGGACTAAAACTTTCCTCTTCAGTTTGCTCGCTGAGTGGATTTTTTTGGCTGATGTATTTACGTTCAAAATATTCATTTGCAACAAAAGCTTAGATTATTTTCAACGAATGTGTACTTTACAGAGAGATTATTCAATGATATACAGTAATCAAGTAAGTATAATTACCCTGCATCAAATTATAGAACTAATTGGCAACAGTATTCATGGAATCGACATTATGGTCGGGAACAGTCTGCGGTGTCGTTGTTTCTTCTGCTGTTTCAGGAACAGAGTGTACTCTGGATTGTCTTGTTCGCGGAGAAGAAGAGTGGAAGTGTTCCTGGCAAAAAAGCTTGCCCATTTCAACAAATTTGGAGGTGAGTGGAGACTGGACGCTGGCGGAGTCCGGCCAACGTGTGATAAGGAACTGGGCCAAATATATTAATAACGAAGACCAGGTAGCAACTGCCGTAATGATGAACAACCCACCAAATCTGTAAACATCCAGAGCTGAATCGTTGGGTTCTGGTTTTGGTGGTTCTTGATTTGAGAAGTATTTCTGCTCAATTGGGCGCATTTTGTCTTGATCTTCGGTCACGTTCAGTATTGCCCTTGAAAAGTCAGCCACCAGAGGGGATCCTTTCGGGAAGGCCTGGATTAATTTTGATAGAACCCAGAAGGCAAAATCGTGAAAATTACGCTCTTAACAGAAGTAAGTTCAAAACGAGAGAGAGAGAAGGCGAAAGAACTCACGAATCCAAAACCACCGGTGCTGTAAATAGGTCCGACCATTTGATAGCCGGAAGAATATTTCCGAAGAAAGACCTTAATGTAAGGAATTTCGTCA

mRNA sequence

ATGGATCATTCCAGAGACGGTGGCGACCAGGTTAGTATAGCAGCTCGCTCCTCGTTATTCGACTCACTGAAAGGATGCGGCCTGTCGGGGATCCCAATCGACAAACAGGAGCTCAATACAAAGGTTATGATGCCGCAGTATCTCCGCTTGGCCTTGCGTGATTCTATCCGCTTAAAAGACCCTACTACAGGAGCCTCTGCCTCTGTTCCACCACAAAATATAGTCCCGCCACTGCCAGAGGCTCCTTTGGTGGTCTTCATTAATCCGCGTAGCGGCGGCCGCCATGGCCAGAAACTCAAGCAGCGCCTCCAGCAATTAATCAGCGAAGAACAGGTTTTTGATCTTTTGGAAGTGAAGCCTCATGAATTTGTACAATATGGACTGCGCTGCCTGGAAATCTTGGCTGATCAAGGTGACATCTGTGCTAAACAAACTCGTCAGAGGATGAGGGTCATGGTTGCAGGAGGCGATGGTACTGTCGGTTGGGTACTGGGAAGTCTAGCAGAACTTTACAAACAAGACCGCAAGCCTGTTCTTCCTGTTGGGATTATCCCTCTTGGTACTGGCAATGATCTTTCCAGGAGTTTTGGTTGGGGAGGTTCATTTCCTTTTGCCTGGCAATCAGCTGTTAAAAGGTCTCTCCACAGGGCTACCACTGGCCAAGTTGGTTATTTGGATAGTTGGCATGTTTTGCTGTCGGTGCCTGCTGGGGAGAAAGTGGAACCACCTCATTCTTTGAAGCTTACAGAAGAAAGTGATCTGAATGAGGCTAAACTGGACGAAAAAGGAGATTTACCAACAAAACTGGCTTCCTATGAAGGAGTATTTTATAATTACTTCAGCATAGGAATGGATGCTCAAGTTGCTTACGGCTTCCATCATTTGCGTAACAAAAGACCATACCTTGCTCAAGGTCCACTTACAAATAAGTCTGTCACTCTTACTGATCCTTTGTTGCTTGGGCTGTTGTGTAGTGTTAGGTCTATTGTTACTTTAAACCTTCATAACTATGCAAGCGGAAGAAACCCATGGGGTAAGCCCAAGCCACAGTACTTGGAGAAGAGAGGCTTTGTGGAGGCGCATGCTGATGATGGTCTTCTAGAGATTTTTGGCCTTAAACAAGGATGGCATACATCGTTCGTTATGGTAGACATAATCTCCGCCAAACACATCGCCCAGGCAGCAGCCATTAGAATAGAAATCAGAGGTGGGAAGCGGAAGAAGGCATATCTGCAGATGGATGGGGAGCCATGGAAACAGCCAATCAATAAGGACTTCTCAACGTATGTTGAAATCAAGAGGGTTCCTTTTCAATCACTAATGATCAACGGGGACTAAAACTTTCCTCTTCAGTTTGCTCGCTGAGTGGATTTTTTTGGCTGATGTATTTACGTTCAAAATATTCATTTGCAACAAAAGCTTAGATTATTTTCAACGAATGTGTACTTTACAGAGAGATTATTCAATGATATACAGTAATCAAGTAAGTATAATTACCCTGCATCAAATTATAGAACTAATTGGCAACAGTATTCATGGAATCGACATTATGGTCGGGAACAGTCTGCGGTGTCGTTGTTTCTTCTGCTGTTTCAGGAACAGAGTGTACTCTGGATTGTCTTGTTCGCGGAGAAGAAGAGTGGAAGTGTTCCTGGCAAAAAAGCTTGCCCATTTCAACAAATTTGGAGGTGAGTGGAGACTGGACGCTGGCGGAGTCCGGCCAACGTGTGATAAGGAACTGGGCCAAATATATTAATAACGAAGACCAGGTAGCAACTGCCGTAATGATGAACAACCCACCAAATCTGTAAACATCCAGAGCTGAATCGTTGGGTTCTGGTTTTGGTGGTTCTTGATTTGAGAAGTATTTCTGCTCAATTGGGCGCATTTTGTCTTGATCTTCGGTCACGTTCAGTATTGCCCTTGAAAAAAGTAAGTTCAAAACGAGAGAGAGAGAAGGCGAAAGAACTCACGAATCCAAAACCACCGGTGCTGTAAATAGGTCCGACCATTTGATAGCCGGAAGAATATTTCCGAAGAAAGACCTTAATGTAAGGAATTTCGTCA

Coding sequence (CDS)

ATGGATCATTCCAGAGACGGTGGCGACCAGGTTAGTATAGCAGCTCGCTCCTCGTTATTCGACTCACTGAAAGGATGCGGCCTGTCGGGGATCCCAATCGACAAACAGGAGCTCAATACAAAGGTTATGATGCCGCAGTATCTCCGCTTGGCCTTGCGTGATTCTATCCGCTTAAAAGACCCTACTACAGGAGCCTCTGCCTCTGTTCCACCACAAAATATAGTCCCGCCACTGCCAGAGGCTCCTTTGGTGGTCTTCATTAATCCGCGTAGCGGCGGCCGCCATGGCCAGAAACTCAAGCAGCGCCTCCAGCAATTAATCAGCGAAGAACAGGTTTTTGATCTTTTGGAAGTGAAGCCTCATGAATTTGTACAATATGGACTGCGCTGCCTGGAAATCTTGGCTGATCAAGGTGACATCTGTGCTAAACAAACTCGTCAGAGGATGAGGGTCATGGTTGCAGGAGGCGATGGTACTGTCGGTTGGGTACTGGGAAGTCTAGCAGAACTTTACAAACAAGACCGCAAGCCTGTTCTTCCTGTTGGGATTATCCCTCTTGGTACTGGCAATGATCTTTCCAGGAGTTTTGGTTGGGGAGGTTCATTTCCTTTTGCCTGGCAATCAGCTGTTAAAAGGTCTCTCCACAGGGCTACCACTGGCCAAGTTGGTTATTTGGATAGTTGGCATGTTTTGCTGTCGGTGCCTGCTGGGGAGAAAGTGGAACCACCTCATTCTTTGAAGCTTACAGAAGAAAGTGATCTGAATGAGGCTAAACTGGACGAAAAAGGAGATTTACCAACAAAACTGGCTTCCTATGAAGGAGTATTTTATAATTACTTCAGCATAGGAATGGATGCTCAAGTTGCTTACGGCTTCCATCATTTGCGTAACAAAAGACCATACCTTGCTCAAGGTCCACTTACAAATAAGTCTGTCACTCTTACTGATCCTTTGTTGCTTGGGCTGTTGTGTAGTGTTAGGTCTATTGTTACTTTAAACCTTCATAACTATGCAAGCGGAAGAAACCCATGGGGTAAGCCCAAGCCACAGTACTTGGAGAAGAGAGGCTTTGTGGAGGCGCATGCTGATGATGGTCTTCTAGAGATTTTTGGCCTTAAACAAGGATGGCATACATCGTTCGTTATGGTAGACATAATCTCCGCCAAACACATCGCCCAGGCAGCAGCCATTAGAATAGAAATCAGAGGTGGGAAGCGGAAGAAGGCATATCTGCAGATGGATGGGGAGCCATGGAAACAGCCAATCAATAAGGACTTCTCAACGTATGTTGAAATCAAGAGGGTTCCTTTTCAATCACTAATGATCAACGGGGACTAA

Protein sequence

MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKVEPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPLTNKSVTLTDPLLLGLLCSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD
Homology
BLAST of Carg15263 vs. NCBI nr
Match: KAG7023409.1 (Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 907.9 bits (2345), Expect = 3.4e-260
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60
           MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120
           PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP
Sbjct: 61  PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP 300
           EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP
Sbjct: 241 EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP 300

Query: 301 YLAQGPLTNKSVTLTDPLLLGLLCSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEA 360
           YLAQGPLTNKSVTLTDPLLLGLLCSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEA
Sbjct: 301 YLAQGPLTNKSVTLTDPLLLGLLCSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEA 360

Query: 361 HADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQ 420
           HADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQ
Sbjct: 361 HADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQ 420

Query: 421 PINKDFSTYVEIKRVPFQSLMINGD 446
           PINKDFSTYVEIKRVPFQSLMINGD
Sbjct: 421 PINKDFSTYVEIKRVPFQSLMINGD 445

BLAST of Carg15263 vs. NCBI nr
Match: KAG6589732.1 (Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 862.1 bits (2226), Expect = 2.2e-246
Identity = 435/480 (90.62%), Postives = 436/480 (90.83%), Query Frame = 0

Query: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60
           MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120
           PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP
Sbjct: 61  PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVG LDSWHVLLSVPAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP 300
           EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP
Sbjct: 241 EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP 300

Query: 301 YLAQGPLTNKSV--------------TLTDPLLLGLL---------------------CS 360
           YLAQGPLTNK +                 DP L GL                       S
Sbjct: 301 YLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSS 360

Query: 361 VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI 420
           VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI
Sbjct: 361 VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI 420

Query: 421 ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 446
           ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD
Sbjct: 421 ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

BLAST of Carg15263 vs. NCBI nr
Match: XP_022921758.1 (diacylglycerol kinase 7-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 858.2 bits (2216), Expect = 3.1e-245
Identity = 434/480 (90.42%), Postives = 435/480 (90.62%), Query Frame = 0

Query: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60
           MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120
           PTTGASASVP QNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP
Sbjct: 61  PTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVG LDSWHVLLSVPAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP 300
           EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP
Sbjct: 241 EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP 300

Query: 301 YLAQGPLTNKSV--------------TLTDPLLLGLL---------------------CS 360
           YLAQGPLTNK +                 DP L GL                       S
Sbjct: 301 YLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSS 360

Query: 361 VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI 420
           VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI
Sbjct: 361 VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI 420

Query: 421 ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 446
           ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD
Sbjct: 421 ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

BLAST of Carg15263 vs. NCBI nr
Match: XP_023516209.1 (diacylglycerol kinase 7-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 857.8 bits (2215), Expect = 4.1e-245
Identity = 433/480 (90.21%), Postives = 434/480 (90.42%), Query Frame = 0

Query: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60
           MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120
           PT GASASVPPQNIVPP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP
Sbjct: 61  PTAGASASVPPQNIVPPQPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVG LDSWHVLLSVPAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP 300
           EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP
Sbjct: 241 EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP 300

Query: 301 YLAQGPLTNKSV--------------TLTDPLLLGLL---------------------CS 360
           YLAQGPLTNK +                 DP L GL                       S
Sbjct: 301 YLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSS 360

Query: 361 VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI 420
           VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI
Sbjct: 361 VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI 420

Query: 421 ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 446
           ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD
Sbjct: 421 ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

BLAST of Carg15263 vs. NCBI nr
Match: XP_022921757.1 (diacylglycerol kinase 7-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 853.2 bits (2203), Expect = 1.0e-243
Identity = 434/482 (90.04%), Postives = 435/482 (90.25%), Query Frame = 0

Query: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60
           MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120
           PTTGASASVP QNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP
Sbjct: 61  PTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVG LDSWHVLLSVPAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHSLKLTEESDLNEA  KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPLTNKSV--------------TLTDPLLLGLL--------------------- 360
           RPYLAQGPLTNK +                 DP L GL                      
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 CSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420
            SVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 446
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

BLAST of Carg15263 vs. ExPASy Swiss-Prot
Match: F4JQ95 (Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 4.0e-163
Identity = 284/463 (61.34%), Postives = 349/463 (75.38%), Query Frame = 0

Query: 22  SLKGCG---LSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASASVPPQNIVPPL 81
           +++GCG   L+ + IDK+EL  ++ MP+YLR+A+RD I+ KD T  +   + P      +
Sbjct: 30  TMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLLPGGAAADM 89

Query: 82  -PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGLRCLEILADQ 141
            P AP+VVFINP+SGGRHG  LK+RLQQL++EEQVFDL EVKPHEFV+YGL CL+ LA +
Sbjct: 90  APHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAK 149

Query: 142 GDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFG 201
           GD CA++ R+++R+MVAGGDGTVGWVLG L EL+K  +  + PVG+IPLGTGNDLSRSF 
Sbjct: 150 GDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFS 209

Query: 202 WGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKVEPPHSLKLTEESDLNEA 261
           WGGSFPFAW+SA+KR+LHRAT G +  LDSW +++S+P+GE V+PP+SLK T E    + 
Sbjct: 210 WGGSFPFAWRSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQ 269

Query: 262 KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPLTNKSV----- 321
            LD  GD+P K  SYEGVFYNYFSIGMDAQVAYGFHHLRN++PYLAQGP+TNK +     
Sbjct: 270 ALDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYS 329

Query: 322 ---------TLTDPLLLGLL-----------C----------SVRSIVTLNLHNYASGRN 381
                     + +P L GL            C          SVRSIV LNL+NY SGR+
Sbjct: 330 CTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSGRH 389

Query: 382 PWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIR 441
           PWG  +P+YLEKRGFVEAH DDGL+EIFGLKQGWH SFVM +IISAKHIAQAAAIR E+R
Sbjct: 390 PWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFELR 449

Query: 442 GGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 446
           GG  K A+LQMDGEPWKQP+  D+ST+VEIK+VPFQSLMING+
Sbjct: 450 GGDWKNAFLQMDGEPWKQPMKSDYSTFVEIKKVPFQSLMINGE 492

BLAST of Carg15263 vs. ExPASy Swiss-Prot
Match: Q8VZG1 (Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 2.5e-160
Identity = 288/486 (59.26%), Postives = 354/486 (72.84%), Query Frame = 0

Query: 6   DGGDQVSIAARSSLFDS--LKGCGLSG---IPIDKQELNTKVMMPQYLRLALRDSIRLKD 65
           D   +  +A+R S  DS  ++GCGL+    + +DK EL  ++MMP+YLRLA+RD I+ KD
Sbjct: 9   DASKEKFVASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAMRDCIKRKD 68

Query: 66  PTTGASASVPPQNIVP------PLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFD 125
                S+++P   ++P        P AP+VVFINP SGGRHG  LK+RLQQL+SEEQVFD
Sbjct: 69  -----SSAIPDHLLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQVFD 128

Query: 126 LLEVKPHEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQD 185
           L EVKPHEFV+YGL CLE +A +GD CAK+ R R+R+MVAGGDGTVGWVLG L EL K  
Sbjct: 129 LTEVKPHEFVRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDG 188

Query: 186 RKPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSV 245
           +  + PVG+IPLGTGNDLSRSFGWGGSFPFAW+SAVKR+LHRA+ G V  LDSW +L+S+
Sbjct: 189 KSQIPPVGVIPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKILVSM 248

Query: 246 PAGEKVEPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHH 305
           P+GE V+PP+SLK  EE++L++  LD   D P    +YEGVFYNY SIGMDAQVAYGFHH
Sbjct: 249 PSGEVVDPPYSLKPAEENELDQG-LDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHH 308

Query: 306 LRNKRPYLAQGPLTNKSV--------------TLTDPLLLGLL-----------CS---- 365
           LRN +PYLAQGP++NK +               + DP L GL            CS    
Sbjct: 309 LRNTKPYLAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEE 368

Query: 366 ------VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTS 425
                 VRSIV LNLH+Y SG +PWG  KP YLEKRGFVEAH DDGL+EIFG KQGWH S
Sbjct: 369 IAVPKNVRSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHAS 428

Query: 426 FVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQS 446
           FVM ++ISAKHIAQAAA+R E+RGG  + A+LQMDGEPWKQP++ ++ST+VEIK+VP+QS
Sbjct: 429 FVMAELISAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEIKKVPYQS 488

BLAST of Carg15263 vs. ExPASy Swiss-Prot
Match: Q1PDI2 (Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 2.5e-157
Identity = 274/454 (60.35%), Postives = 340/454 (74.89%), Query Frame = 0

Query: 28  LSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASASV-PPQNIVPPLPEAPLVVF 87
           L+ I + K EL  +VM+PQYLR+A+RD I  KD +  AS+SV PP       PE PL+VF
Sbjct: 35  LASIRVSKAELRQRVMLPQYLRIAIRDCILRKDDSFDASSSVAPPLENNALTPEVPLMVF 94

Query: 88  INPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGLRCLEILADQGDICAKQTR 147
           +NP+SGGR G  +K+RLQ LISEEQV+DL EVKP+EF++YGL CLE  A +GD CAK+ R
Sbjct: 95  VNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIR 154

Query: 148 QRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFGWGGSFPFAW 207
           ++MR++VAGGDGTVGWVLG L EL  Q+R PV PV I+PLGTGNDLSRSFGWGGSFPFAW
Sbjct: 155 EKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAW 214

Query: 208 QSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKVEPPHSLKLTEESDLNEAKLDEKGDLP 267
           +SA+KR+LHRA+   +  LDSW++L+++P+GE V+PP+SLK T+E  +++  L+ +G++P
Sbjct: 215 KSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLKATQECYIDQ-NLEIEGEIP 274

Query: 268 TKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPLTNKSV-------------- 327
                YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLA GP+ NK +              
Sbjct: 275 PSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTH 334

Query: 328 TLTDPLLLGLL---------------------CSVRSIVTLNLHNYASGRNPWGKPKPQY 387
            + DP L GL                       SVR++V LNLH+Y SGRNPWG  K  Y
Sbjct: 335 CINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDY 394

Query: 388 LEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYL 446
           LEKRGFVEA ADDGLLEIFGLKQGWH SFVMV++ISAKHIAQAAAIR+EIRGG  K A++
Sbjct: 395 LEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFM 454

BLAST of Carg15263 vs. ExPASy Swiss-Prot
Match: Q7XQT2 (Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 232.3 bits (591), Expect = 1.1e-59
Identity = 146/402 (36.32%), Postives = 210/402 (52.24%), Query Frame = 0

Query: 72  QNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGLRC- 131
           Q +V   P  P+VVFIN RSGG+ G  L +  ++L+++ QVFDL E  P E V + L C 
Sbjct: 35  QVLVDQAPCCPVVVFINSRSGGQLGSSLIKTYRELLNKAQVFDLSEEAP-EKVLHRLYCN 94

Query: 132 LEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGN 191
            E L   GD  A Q +  +R++VAGGDGT  W+LG +++L K    P  P+  +PLGTGN
Sbjct: 95  FEKLKSNGDPIAFQIQSNLRLIVAGGDGTASWLLGVVSDL-KLSHPP--PIATVPLGTGN 154

Query: 192 DLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEK--------VEP 251
           +L  SFGWG   P   Q AVK  L +    +   +DSWH+++ + A ++        +E 
Sbjct: 155 NLPFSFGWGKKNPTTDQEAVKSFLGQVKKAREMNIDSWHIIMRMRAPQEGPCEPIAPLEL 214

Query: 252 PHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYL 311
           PHSL        +++ L+ +G       +Y G F+NYFS+GMDAQV+Y FH  R + P  
Sbjct: 215 PHSLHAFHRVSGSDS-LNMEG-----YHTYRGGFWNYFSMGMDAQVSYEFHSERKRNPEK 274

Query: 312 AQGPLTNKS-------------VTLTDPLL---------------------LGLLCSVRS 371
            +  LTN+S              +LT P                       L +  S+RS
Sbjct: 275 FKNQLTNQSTYAKLGLKQGWFAASLTHPSSRNIAQLAKVRIMKRPGGQWEELKIPRSIRS 334

Query: 372 IVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISA 431
           IV LNL +++ G NPWG P  + ++ R       DDGL+E+ G +  WH   ++      
Sbjct: 335 IVCLNLPSFSGGLNPWGTPGTRKVQDRDLTAPFVDDGLIEVVGFRDAWHGLVLLAPNGHG 394

BLAST of Carg15263 vs. ExPASy Swiss-Prot
Match: Q9C5E5 (Diacylglycerol kinase 5 OS=Arabidopsis thaliana OX=3702 GN=DGK5 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 3.5e-58
Identity = 137/412 (33.25%), Postives = 210/412 (50.97%), Query Frame = 0

Query: 77  PLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGLRCLEILAD 136
           P P +P++VFIN +SGG+ G +L    + L++  QVFDL +  P + ++     LE L D
Sbjct: 35  PTPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLKD 94

Query: 137 QGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSF 196
             D  A+Q R++++++VAGGDGT GW+LG + +L K    P  P+  +PLGTGN+L  +F
Sbjct: 95  --DDFARQIREKLKIIVAGGDGTAGWLLGVVCDL-KLSHPP--PIATVPLGTGNNLPFAF 154

Query: 197 GWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEK---------VEPPHSLK 256
           GWG   P   ++AV+  L +    +V  +D+WH+L+ +   ++         +E PHSL 
Sbjct: 155 GWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPHSLH 214

Query: 257 LTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPL 316
                   + +L+++G       ++ G F+NYFS+GMDAQ++Y FH  R   P   +  L
Sbjct: 215 AFHRVSPTD-ELNKEG-----CHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKNQL 274

Query: 317 TNKSVTLTDPLLLGLLC---------------------------------SVRSIVTLNL 376
            N+S  +      G  C                                 S+RSIV LNL
Sbjct: 275 VNQSTYVKLGCTQGWFCASLFHPASRNIAQLAKVKIATRNGQWQDLHIPHSIRSIVCLNL 334

Query: 377 HNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQA 436
            +++ G NPWG P P+    RG      DDGL+E+ G +  WH   ++        +AQA
Sbjct: 335 PSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLAQA 394

Query: 437 AAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFST-YVEIKRVPFQSLMINGD 446
             IR E   G     +++MDGEPWKQP+  D  T  VEI  +   +++   D
Sbjct: 395 NRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNMLATHD 435

BLAST of Carg15263 vs. ExPASy TrEMBL
Match: A0A6J1E4R5 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 1.5e-245
Identity = 434/480 (90.42%), Postives = 435/480 (90.62%), Query Frame = 0

Query: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60
           MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120
           PTTGASASVP QNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP
Sbjct: 61  PTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVG LDSWHVLLSVPAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP 300
           EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP
Sbjct: 241 EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP 300

Query: 301 YLAQGPLTNKSV--------------TLTDPLLLGLL---------------------CS 360
           YLAQGPLTNK +                 DP L GL                       S
Sbjct: 301 YLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSS 360

Query: 361 VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI 420
           VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI
Sbjct: 361 VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI 420

Query: 421 ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 446
           ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD
Sbjct: 421 ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

BLAST of Carg15263 vs. ExPASy TrEMBL
Match: A0A6J1E1C6 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 4.8e-244
Identity = 434/482 (90.04%), Postives = 435/482 (90.25%), Query Frame = 0

Query: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60
           MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120
           PTTGASASVP QNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP
Sbjct: 61  PTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVG LDSWHVLLSVPAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHSLKLTEESDLNEA  KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPLTNKSV--------------TLTDPLLLGLL--------------------- 360
           RPYLAQGPLTNK +                 DP L GL                      
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 CSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420
            SVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 446
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

BLAST of Carg15263 vs. ExPASy TrEMBL
Match: A0A6J1JHE1 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 2.9e-241
Identity = 426/480 (88.75%), Postives = 431/480 (89.79%), Query Frame = 0

Query: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60
           MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLA+RDSIRLKD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLAMRDSIRLKD 60

Query: 61  PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120
           PT GASAS+PPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP
Sbjct: 61  PTAGASASLPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAEL  QDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELNNQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVG LDSWHVLLSVPAGE+V
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGERV 240

Query: 241 EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRP 300
           EPPHSLKLTEESDLNEAKLDEKGDLPTKLASY GVFYNYFSIGMDAQVAYGFHHLRNKRP
Sbjct: 241 EPPHSLKLTEESDLNEAKLDEKGDLPTKLASYGGVFYNYFSIGMDAQVAYGFHHLRNKRP 300

Query: 301 YLAQGPLTNKSV--------------TLTDPLLLGLL---------------------CS 360
            LA+GPLTNK +                 DP L GL                       S
Sbjct: 301 KLARGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSS 360

Query: 361 VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI 420
           VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI
Sbjct: 361 VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDI 420

Query: 421 ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 446
           ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD
Sbjct: 421 ISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

BLAST of Carg15263 vs. ExPASy TrEMBL
Match: A0A6J1JJR6 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 9.5e-240
Identity = 426/482 (88.38%), Postives = 431/482 (89.42%), Query Frame = 0

Query: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60
           MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLA+RDSIRLKD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLAMRDSIRLKD 60

Query: 61  PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120
           PT GASAS+PPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP
Sbjct: 61  PTAGASASLPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAEL  QDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELNNQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVG LDSWHVLLSVPAGE+V
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGERV 240

Query: 241 EPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHSLKLTEESDLNEA  KLDEKGDLPTKLASY GVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEAVRKLDEKGDLPTKLASYGGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPLTNKSV--------------TLTDPLLLGLL--------------------- 360
           RP LA+GPLTNK +                 DP L GL                      
Sbjct: 301 RPKLARGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 CSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420
            SVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 446
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

BLAST of Carg15263 vs. ExPASy TrEMBL
Match: A0A0A0LZ78 (Diacylglycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_1G295150 PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.3e-217
Identity = 388/485 (80.00%), Postives = 412/485 (84.95%), Query Frame = 0

Query: 1   MDHSRDG---GDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIR 60
           MDHS  G   GD+V++AARSS  DSLKGCGLSGI IDK ELNTKV+MPQYLR ALRDSIR
Sbjct: 105 MDHSSAGPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIR 164

Query: 61  LKDPTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLE 120
           LKDP  GASA+ PPQN+V PLPE PLVVFINPRSGGRHGQKLKQRLQ+LISEEQVFDLL+
Sbjct: 165 LKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLD 224

Query: 121 VKPHEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKP 180
           VKPHEFVQYGLRCLEILAD GD+CAKQTRQ++R+MVAGGDGTVGWVLGSLAELYKQDR P
Sbjct: 225 VKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNP 284

Query: 181 VLPVGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAG 240
           VLPVGIIPLGTGNDL+RSFGWGGSFPFAW+SAVKRSL RATTGQVG LDSWHV LS+PAG
Sbjct: 285 VLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMPAG 344

Query: 241 EKVEPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHL 300
           EKVEPPHSLKLTEESDLNEA  KLD+  D PTKL  +EGVFYNYFSIGMDAQVAYGFHHL
Sbjct: 345 EKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFHHL 404

Query: 301 RNKRPYLAQGPLTNKSV--------------TLTDPLLLGLL------------------ 360
           RNKRPYLAQGP+TNK +                 +P L GL                   
Sbjct: 405 RNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWEKV 464

Query: 361 ---CSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSF 420
               SVRSIV LNLHNYASGRNPWGK KP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSF
Sbjct: 465 PVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSF 524

Query: 421 VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSL 446
           VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+K+ ST+VEIKRVPFQSL
Sbjct: 525 VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQSL 584

BLAST of Carg15263 vs. TAIR 10
Match: AT4G30340.1 (diacylglycerol kinase 7 )

HSP 1 Score: 575.9 bits (1483), Expect = 2.9e-164
Identity = 284/463 (61.34%), Postives = 349/463 (75.38%), Query Frame = 0

Query: 22  SLKGCG---LSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASASVPPQNIVPPL 81
           +++GCG   L+ + IDK+EL  ++ MP+YLR+A+RD I+ KD T  +   + P      +
Sbjct: 30  TMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLLPGGAAADM 89

Query: 82  -PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGLRCLEILADQ 141
            P AP+VVFINP+SGGRHG  LK+RLQQL++EEQVFDL EVKPHEFV+YGL CL+ LA +
Sbjct: 90  APHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAK 149

Query: 142 GDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFG 201
           GD CA++ R+++R+MVAGGDGTVGWVLG L EL+K  +  + PVG+IPLGTGNDLSRSF 
Sbjct: 150 GDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFS 209

Query: 202 WGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKVEPPHSLKLTEESDLNEA 261
           WGGSFPFAW+SA+KR+LHRAT G +  LDSW +++S+P+GE V+PP+SLK T E    + 
Sbjct: 210 WGGSFPFAWRSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQ 269

Query: 262 KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPLTNKSV----- 321
            LD  GD+P K  SYEGVFYNYFSIGMDAQVAYGFHHLRN++PYLAQGP+TNK +     
Sbjct: 270 ALDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYS 329

Query: 322 ---------TLTDPLLLGLL-----------C----------SVRSIVTLNLHNYASGRN 381
                     + +P L GL            C          SVRSIV LNL+NY SGR+
Sbjct: 330 CTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSGRH 389

Query: 382 PWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIR 441
           PWG  +P+YLEKRGFVEAH DDGL+EIFGLKQGWH SFVM +IISAKHIAQAAAIR E+R
Sbjct: 390 PWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFELR 449

Query: 442 GGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 446
           GG  K A+LQMDGEPWKQP+  D+ST+VEIK+VPFQSLMING+
Sbjct: 450 GGDWKNAFLQMDGEPWKQPMKSDYSTFVEIKKVPFQSLMINGE 492

BLAST of Carg15263 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 566.6 bits (1459), Expect = 1.7e-161
Identity = 288/486 (59.26%), Postives = 354/486 (72.84%), Query Frame = 0

Query: 6   DGGDQVSIAARSSLFDS--LKGCGLSG---IPIDKQELNTKVMMPQYLRLALRDSIRLKD 65
           D   +  +A+R S  DS  ++GCGL+    + +DK EL  ++MMP+YLRLA+RD I+ KD
Sbjct: 9   DASKEKFVASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAMRDCIKRKD 68

Query: 66  PTTGASASVPPQNIVP------PLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFD 125
                S+++P   ++P        P AP+VVFINP SGGRHG  LK+RLQQL+SEEQVFD
Sbjct: 69  -----SSAIPDHLLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQVFD 128

Query: 126 LLEVKPHEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQD 185
           L EVKPHEFV+YGL CLE +A +GD CAK+ R R+R+MVAGGDGTVGWVLG L EL K  
Sbjct: 129 LTEVKPHEFVRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDG 188

Query: 186 RKPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSV 245
           +  + PVG+IPLGTGNDLSRSFGWGGSFPFAW+SAVKR+LHRA+ G V  LDSW +L+S+
Sbjct: 189 KSQIPPVGVIPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKILVSM 248

Query: 246 PAGEKVEPPHSLKLTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHH 305
           P+GE V+PP+SLK  EE++L++  LD   D P    +YEGVFYNY SIGMDAQVAYGFHH
Sbjct: 249 PSGEVVDPPYSLKPAEENELDQG-LDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHH 308

Query: 306 LRNKRPYLAQGPLTNKSV--------------TLTDPLLLGLL-----------CS---- 365
           LRN +PYLAQGP++NK +               + DP L GL            CS    
Sbjct: 309 LRNTKPYLAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEE 368

Query: 366 ------VRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTS 425
                 VRSIV LNLH+Y SG +PWG  KP YLEKRGFVEAH DDGL+EIFG KQGWH S
Sbjct: 369 IAVPKNVRSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHAS 428

Query: 426 FVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQS 446
           FVM ++ISAKHIAQAAA+R E+RGG  + A+LQMDGEPWKQP++ ++ST+VEIK+VP+QS
Sbjct: 429 FVMAELISAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEIKKVPYQS 488

BLAST of Carg15263 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 556.6 bits (1433), Expect = 1.8e-158
Identity = 274/454 (60.35%), Postives = 340/454 (74.89%), Query Frame = 0

Query: 28  LSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASASV-PPQNIVPPLPEAPLVVF 87
           L+ I + K EL  +VM+PQYLR+A+RD I  KD +  AS+SV PP       PE PL+VF
Sbjct: 35  LASIRVSKAELRQRVMLPQYLRIAIRDCILRKDDSFDASSSVAPPLENNALTPEVPLMVF 94

Query: 88  INPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGLRCLEILADQGDICAKQTR 147
           +NP+SGGR G  +K+RLQ LISEEQV+DL EVKP+EF++YGL CLE  A +GD CAK+ R
Sbjct: 95  VNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIR 154

Query: 148 QRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFGWGGSFPFAW 207
           ++MR++VAGGDGTVGWVLG L EL  Q+R PV PV I+PLGTGNDLSRSFGWGGSFPFAW
Sbjct: 155 EKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAW 214

Query: 208 QSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEKVEPPHSLKLTEESDLNEAKLDEKGDLP 267
           +SA+KR+LHRA+   +  LDSW++L+++P+GE V+PP+SLK T+E  +++  L+ +G++P
Sbjct: 215 KSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLKATQECYIDQ-NLEIEGEIP 274

Query: 268 TKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPLTNKSV-------------- 327
                YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLA GP+ NK +              
Sbjct: 275 PSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTH 334

Query: 328 TLTDPLLLGLL---------------------CSVRSIVTLNLHNYASGRNPWGKPKPQY 387
            + DP L GL                       SVR++V LNLH+Y SGRNPWG  K  Y
Sbjct: 335 CINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDY 394

Query: 388 LEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYL 446
           LEKRGFVEA ADDGLLEIFGLKQGWH SFVMV++ISAKHIAQAAAIR+EIRGG  K A++
Sbjct: 395 LEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFM 454

BLAST of Carg15263 vs. TAIR 10
Match: AT2G20900.1 (diacylglycerol kinase 5 )

HSP 1 Score: 227.3 bits (578), Expect = 2.5e-59
Identity = 137/412 (33.25%), Postives = 210/412 (50.97%), Query Frame = 0

Query: 77  PLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGLRCLEILAD 136
           P P +P++VFIN +SGG+ G +L    + L++  QVFDL +  P + ++     LE L D
Sbjct: 35  PTPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLKD 94

Query: 137 QGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSF 196
             D  A+Q R++++++VAGGDGT GW+LG + +L K    P  P+  +PLGTGN+L  +F
Sbjct: 95  --DDFARQIREKLKIIVAGGDGTAGWLLGVVCDL-KLSHPP--PIATVPLGTGNNLPFAF 154

Query: 197 GWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEK---------VEPPHSLK 256
           GWG   P   ++AV+  L +    +V  +D+WH+L+ +   ++         +E PHSL 
Sbjct: 155 GWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPHSLH 214

Query: 257 LTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPL 316
                   + +L+++G       ++ G F+NYFS+GMDAQ++Y FH  R   P   +  L
Sbjct: 215 AFHRVSPTD-ELNKEG-----CHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKNQL 274

Query: 317 TNKSVTLTDPLLLGLLC---------------------------------SVRSIVTLNL 376
            N+S  +      G  C                                 S+RSIV LNL
Sbjct: 275 VNQSTYVKLGCTQGWFCASLFHPASRNIAQLAKVKIATRNGQWQDLHIPHSIRSIVCLNL 334

Query: 377 HNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQA 436
            +++ G NPWG P P+    RG      DDGL+E+ G +  WH   ++        +AQA
Sbjct: 335 PSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLAQA 394

Query: 437 AAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFST-YVEIKRVPFQSLMINGD 446
             IR E   G     +++MDGEPWKQP+  D  T  VEI  +   +++   D
Sbjct: 395 NRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNMLATHD 435

BLAST of Carg15263 vs. TAIR 10
Match: AT2G20900.3 (diacylglycerol kinase 5 )

HSP 1 Score: 227.3 bits (578), Expect = 2.5e-59
Identity = 137/412 (33.25%), Postives = 210/412 (50.97%), Query Frame = 0

Query: 77  PLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGLRCLEILAD 136
           P P +P++VFIN +SGG+ G +L    + L++  QVFDL +  P + ++     LE L D
Sbjct: 35  PTPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLKD 94

Query: 137 QGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSF 196
             D  A+Q R++++++VAGGDGT GW+LG + +L K    P  P+  +PLGTGN+L  +F
Sbjct: 95  --DDFARQIREKLKIIVAGGDGTAGWLLGVVCDL-KLSHPP--PIATVPLGTGNNLPFAF 154

Query: 197 GWGGSFPFAWQSAVKRSLHRATTGQVGYLDSWHVLLSVPAGEK---------VEPPHSLK 256
           GWG   P   ++AV+  L +    +V  +D+WH+L+ +   ++         +E PHSL 
Sbjct: 155 GWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPHSLH 214

Query: 257 LTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPL 316
                   + +L+++G       ++ G F+NYFS+GMDAQ++Y FH  R   P   +  L
Sbjct: 215 AFHRVSPTD-ELNKEG-----CHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKNQL 274

Query: 317 TNKSVTLTDPLLLGLLC---------------------------------SVRSIVTLNL 376
            N+S  +      G  C                                 S+RSIV LNL
Sbjct: 275 VNQSTYVKLGCTQGWFCASLFHPASRNIAQLAKVKIATRNGQWQDLHIPHSIRSIVCLNL 334

Query: 377 HNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQA 436
            +++ G NPWG P P+    RG      DDGL+E+ G +  WH   ++        +AQA
Sbjct: 335 PSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLAQA 394

Query: 437 AAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFST-YVEIKRVPFQSLMINGD 446
             IR E   G     +++MDGEPWKQP+  D  T  VEI  +   +++   D
Sbjct: 395 NRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNMLATHD 435

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7023409.13.4e-260100.00Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6589732.12.2e-24690.63Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022921758.13.1e-24590.42diacylglycerol kinase 7-like isoform X2 [Cucurbita moschata][more]
XP_023516209.14.1e-24590.21diacylglycerol kinase 7-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022921757.11.0e-24390.04diacylglycerol kinase 7-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
F4JQ954.0e-16361.34Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1[more]
Q8VZG12.5e-16059.26Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1[more]
Q1PDI22.5e-15760.35Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1[more]
Q7XQT21.1e-5936.32Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV... [more]
Q9C5E53.5e-5833.25Diacylglycerol kinase 5 OS=Arabidopsis thaliana OX=3702 GN=DGK5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E4R51.5e-24590.42diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1[more]
A0A6J1E1C64.8e-24490.04diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1[more]
A0A6J1JHE12.9e-24188.75diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1[more]
A0A6J1JJR69.5e-24088.38diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1[more]
A0A0A0LZ781.3e-21780.00Diacylglycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_1G295150 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G30340.12.9e-16461.34diacylglycerol kinase 7 [more]
AT2G18730.11.7e-16159.26diacylglycerol kinase 3 [more]
AT5G57690.11.8e-15860.35diacylglycerol kinase 4 [more]
AT2G20900.12.5e-5933.25diacylglycerol kinase 5 [more]
AT2G20900.32.5e-5933.25diacylglycerol kinase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000756Diacylglycerol kinase, accessory domainSMARTSM00045dagk_c4b_2coord: 275..417
e-value: 2.7E-7
score: 9.0
IPR000756Diacylglycerol kinase, accessory domainPFAMPF00609DAGK_acccoord: 275..312
e-value: 4.7E-11
score: 43.3
coord: 325..417
e-value: 3.4E-19
score: 69.7
IPR001206Diacylglycerol kinase, catalytic domainSMARTSM00046dagk_c4a_7coord: 82..228
e-value: 3.5E-28
score: 109.6
IPR001206Diacylglycerol kinase, catalytic domainPFAMPF00781DAGK_catcoord: 82..222
e-value: 1.5E-31
score: 108.7
IPR001206Diacylglycerol kinase, catalytic domainPROSITEPS50146DAGKcoord: 78..237
score: 27.336309
NoneNo IPR availableGENE3D2.60.200.40coord: 273..424
e-value: 1.4E-5
score: 27.0
NoneNo IPR availablePANTHERPTHR11255:SF96DIACYLGLYCEROL KINASEcoord: 324..444
NoneNo IPR availablePANTHERPTHR11255:SF96DIACYLGLYCEROL KINASEcoord: 7..312
IPR017438Inorganic polyphosphate/ATP-NAD kinase, N-terminalGENE3D3.40.50.10330coord: 80..225
e-value: 1.4E-23
score: 84.8
IPR037607Diacylglycerol kinasePANTHERPTHR11255DIACYLGLYCEROL KINASEcoord: 7..312
IPR037607Diacylglycerol kinasePANTHERPTHR11255DIACYLGLYCEROL KINASEcoord: 324..444
IPR016064NAD kinase/diacylglycerol kinase-like domain superfamilySUPERFAMILY111331NAD kinase/diacylglycerol kinase-likecoord: 82..423

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15263-RACarg15263-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0016310 phosphorylation
biological_process GO:0007205 protein kinase C-activating G protein-coupled receptor signaling pathway
biological_process GO:0007165 signal transduction
molecular_function GO:0005524 ATP binding
molecular_function GO:0004143 diacylglycerol kinase activity
molecular_function GO:0003951 NAD+ kinase activity
molecular_function GO:0016301 kinase activity