Carg15150 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg15150
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	respiratory burst oxidase homolog protein F-like
Location	Carg_Chr10: 1359625 .. 1361355 (+)
RNA-Seq Expression	Carg15150
Synteny	Carg15150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGCATTGTAGCAGGAGTTATCCTTTCATGGAGGAACCCATCTTGTTTGTGATTTTCCAAGAATTAGTAGATGTCTATCCAATGTCTATCCAACAAAGCAACTGAAAAAACGGTAAAGCCAGTTTTCTGAGCTCAACTTTTGTATGTGTGTCAAAGATTCTATTTGAATTTCTGCATCATCCATTGATCATGAGGTGATAATATTTCAGATAATAGGGGACTTGGATGTCTATAGCAATTCCAGTTTTGTTATATACTGGAGAGAGGGCTTTTAGAGCAATTCGATCTTGTTTGTACCAGGTGAAAGTCTTGAAGGTACTCCTACTTTACTTTCAAATTTAGAGGAGATGAGAGCAACGACCTCAAATATGAGTATTTGACAACAGTTGTATGTTTATCATGTATTATATGCAGGCAAGGACTTAATTCAGGAAAAGTATTATTTCTCCAATTGAGCAAACCAGAGAGATTCAAGTATGTATTAATTCAATGCCCTCAAATCTCCCCATTTGAATGGCAATACGTCCTAATGAGTTTTAGTCTTCTAAGTGTTAGACGAAGACGACTCTCCACAATGGTATGATATTGTCCACTTTGAGCATAAGCTCTCATGGCTTTGCTTTGGGCTTTCCCAAAAGACCTCATACTAATGGAGAGTGTATTCTTAGATTCTAAATCCATGACCATTCCCTAAATTAACCAATGTGGGACACTTTCATCCAACACTAAGTTCTAACCGCTATTCTCATTAATTCTTCCCCACAAGAAACGCACCTCAGCGTGCATATCAGAAATCTGGGAGATTGGAGTAATGAGCTGTACAGCCTGTTTCACAAGGTAATTGTTGAAGGTGATTGGTGGGAGGGTTAAAATTTTACATTTATCTTAAAGCACCATCTTTCCTGCGCTATGGTTTAGTTCGTTAGATAATAAGTAAATAAAGTGTGTTTTTTCATGAGAAAGATTATGAAGGAAAGAAGAGAGAGCTGATGTAAGTACTAGAAACATTCTCTTGGACTAGGTTGAGCATTATAAATTACTGCATAGAGGTTAAAAGGAGTCATGATCAGGCCCAATCTCAATTATACAATATGATTTATAGGGAATATCTCTTGGTTCAAAGGAGTACCCAAAAATATTCATTGATGGACCCTACGGTGCTGCTTCTCAAGACCATGTCAAGTACAACCATGTGGTGCTAATTGGCCTGGGTATTGGAGCCACACCCTTCATGAGCGTCCTAAAAGATGCTCTAAACAGGTTAAGTGAGACATGTGATGGTCATGTAAGACTTCTTCCCCCTATGATTTGATTTCTTCAATATCCTTCCACAACTTACCACCAACAAAGTTTGACTGTGGTTTAAAACTGAGTAGACTGACAAAGGAAGAGGCAGCCTAAGTACATCCAAAATTTATCTTTACTGGGCAAGAGAGCAAAGCTCCTTTGATTGGTTTAAAGATTTCATGAACGATTTAACACTTTCAGACCAAACTCAGGTAAAAGCCATATTACTAAATACTCTAGTCCTGCAAAATATGTTTTGAGATCTGCTGATTTGCTTTTTGTATATTTAAAGCATTCTAATGTCATGATGCTCTTAGTCAGCTGTAGAGATGCACAATTTCCTCACTAGTATATATCATGAAGGGGATGTAAGGTCTGTTCTAATAAGAGCAATTCAAGCCCTGCATTATACTCGAAGGGGCATGGACATTGTCTCCAAAACT

mRNA sequence

GAGGCATTGTAGCAGGAGTTATCCTTTCATGGAGGAACCCATCTTGCAAGGACTTAATTCAGGAAAAGGAATATCTCTTGGTTCAAAGGAGTACCCAAAAATATTCATTGATGGACCCTACGGTGCTGCTTCTCAAGACCATGTCAAGTACAACCATGTGGTGCTAATTGGCCTGGGTATTGGAGCCACACCCTTCATGAGCGTCCTAAAAGATGCTCTAAACAGGTTAAGTGAGACATGTGATGGTCATACTGACAAAGGAAGAGGCAGCCTAAGTACATCCAAAATTTATCTTTACTGGGCAAGAGAGCAAAGCTCCTTTGATTGGTTTAAAGATTTCATGAACGATTTAACACTTTCAGACCAAACTCAGTCAGCTGTAGAGATGCACAATTTCCTCACTAGTATATATCATGAAGGGGATGTAAGGTCTGTTCTAATAAGAGCAATTCAAGCCCTGCATTATACTCGAAGGGGCATGGACATTGTCTCCAAAACT

Coding sequence (CDS)

ATGGAGGAACCCATCTTGCAAGGACTTAATTCAGGAAAAGGAATATCTCTTGGTTCAAAGGAGTACCCAAAAATATTCATTGATGGACCCTACGGTGCTGCTTCTCAAGACCATGTCAAGTACAACCATGTGGTGCTAATTGGCCTGGGTATTGGAGCCACACCCTTCATGAGCGTCCTAAAAGATGCTCTAAACAGGTTAAGTGAGACATGTGATGGTCATACTGACAAAGGAAGAGGCAGCCTAAGTACATCCAAAATTTATCTTTACTGGGCAAGAGAGCAAAGCTCCTTTGATTGGTTTAAAGATTTCATGAACGATTTAACACTTTCAGACCAAACTCAGTCAGCTGTAGAGATGCACAATTTCCTCACTAGTATATATCATGAAGGGGATGTAAGGTCTGTTCTAATAAGAGCAATTCAAGCCCTGCATTATACTCGAAGGGGCATGGACATTGTCTCCAAAACT

Protein sequence

MEEPILQGLNSGKGISLGSKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETCDGHTDKGRGSLSTSKIYLYWAREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEGDVRSVLIRAIQALHYTRRGMDIVSKT

Homology

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BLAST of Carg15150 vs. NCBI nr
Match: KAG7023296.1 (Respiratory burst oxidase-like protein F, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 321.2 bits (822), Expect = 4.8e-84
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 1   MEEPILQGLNSGKGISLGSKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVL 60
           MEEPILQGLNSGKGISLGSKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVL
Sbjct: 1   MEEPILQGLNSGKGISLGSKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVL 60

Query: 61  KDALNRLSETCDGHTDKGRGSLSTSKIYLYWAREQSSFDWFKDFMNDLTLSDQTQSAVEM 120
           KDALNRLSETCDGHTDKGRGSLSTSKIYLYWAREQSSFDWFKDFMNDLTLSDQTQSAVEM
Sbjct: 61  KDALNRLSETCDGHTDKGRGSLSTSKIYLYWAREQSSFDWFKDFMNDLTLSDQTQSAVEM 120

Query: 121 HNFLTSIYHEGDVRSVLIRAIQALHYTRRGMDIVSKT 158
           HNFLTSIYHEGDVRSVLIRAIQALHYTRRGMDIVSKT
Sbjct: 121 HNFLTSIYHEGDVRSVLIRAIQALHYTRRGMDIVSKT 157

[Close]

BLAST of Carg15150 vs. NCBI nr
Match: KAG6589605.1 (Mitochondrial substrate carrier family protein E, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 297.4 bits (760), Expect = 7.5e-77
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 13  KGISLGSKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETCD 72
           KGISLGSKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETCD
Sbjct: 30  KGISLGSKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETCD 89

Query: 73  GHTDKGRGSLSTSKIYLYWAREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEGD 132
           GHTDKGRGSLSTSKIYLYWAREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEGD
Sbjct: 90  GHTDKGRGSLSTSKIYLYWAREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEGD 149

Query: 133 VRSVLIRAIQALHYTRRGMDIVSKT 158
           VRSVLIRAIQALHYTRRGMDIVSKT
Sbjct: 150 VRSVLIRAIQALHYTRRGMDIVSKT 174

[Close]

BLAST of Carg15150 vs. NCBI nr
Match: XP_022921387.1 (respiratory burst oxidase homolog protein F-like [Cucurbita moschata])

HSP 1 Score: 289.7 bits (740), Expect = 1.6e-74
Identity = 142/145 (97.93%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 13  KGISLGSKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETCD 72
           KGISLGSKEYPKIFIDGPYGAASQD VKYNHVVLIGLGIGATPFMSVLKDALNRL+ETCD
Sbjct: 30  KGISLGSKEYPKIFIDGPYGAASQDLVKYNHVVLIGLGIGATPFMSVLKDALNRLTETCD 89

Query: 73  GHTDKGRGSLSTSKIYLYWAREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEGD 132
           GHTDKGRGSLSTSKIYLYWAREQSSFDWFKDFMNDL LSDQTQSAVEMHNFLTSIYHEGD
Sbjct: 90  GHTDKGRGSLSTSKIYLYWAREQSSFDWFKDFMNDLILSDQTQSAVEMHNFLTSIYHEGD 149

Query: 133 VRSVLIRAIQALHYTRRGMDIVSKT 158
           VRSVLIRAIQALHYTRRGMDIVSKT
Sbjct: 150 VRSVLIRAIQALHYTRRGMDIVSKT 174

[Close]

BLAST of Carg15150 vs. NCBI nr
Match: XP_022971705.1 (respiratory burst oxidase homolog protein A-like [Cucurbita maxima] >XP_022971713.1 respiratory burst oxidase homolog protein A-like [Cucurbita maxima])

HSP 1 Score: 251.1 bits (640), Expect = 6.1e-63
Identity = 122/140 (87.14%), Postives = 128/140 (91.43%), Query Frame = 0


Query: 19  SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETCDGHTDKG 78
           SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMS+LKD LNRL+  CDGHTD G
Sbjct: 586 SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSILKDVLNRLTGKCDGHTDNG 645

Query: 79  RGSLSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEGDVRSVL 138
           +GS S SK YLYW  REQSSFDWFKDFMNDLT SDQTQ AVEMHNFLTS++HE DVRSVL
Sbjct: 646 KGSPSKSKTYLYWVTREQSSFDWFKDFMNDLTQSDQTQLAVEMHNFLTSVHHENDVRSVL 705

Query: 139 IRAIQALHYTRRGMDIVSKT 158
           IRAIQALHYTRRG+DIVSKT
Sbjct: 706 IRAIQALHYTRRGIDIVSKT 725

[Close]

BLAST of Carg15150 vs. NCBI nr
Match: KAG6587580.1 (Respiratory burst oxidase-like protein A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 250.4 bits (638), Expect = 1.0e-62
Identity = 122/140 (87.14%), Postives = 128/140 (91.43%), Query Frame = 0


Query: 19  SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETCDGHTDKG 78
           SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMS+LKD LNRL+  CDGHTD G
Sbjct: 472 SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSILKDVLNRLTGKCDGHTDNG 531

Query: 79  RGSLSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEGDVRSVL 138
           +GS S SK YLYW  REQSSFDWFKDFMNDLT SDQTQ AVEMHNFLTS++HE DVRSVL
Sbjct: 532 KGSPSKSKTYLYWVTREQSSFDWFKDFMNDLTHSDQTQLAVEMHNFLTSVHHEDDVRSVL 591

Query: 139 IRAIQALHYTRRGMDIVSKT 158
           IRAIQALHYTRRG+DIVSKT
Sbjct: 592 IRAIQALHYTRRGIDIVSKT 611

[Close]

BLAST of Carg15150 vs. ExPASy Swiss-Prot
Match: O48538 (Respiratory burst oxidase homolog protein F OS=Arabidopsis thaliana OX=3702 GN=RBOHF PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 3.6e-34
Identity = 77/158 (48.73%), Postives = 101/158 (63.92%), Query Frame = 0


Query: 20  KEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALN---RLSETCDGHTD 79
           K  PK+ IDGPYGA +QD+ KY+ ++L+GLGIGATPF+S+LKD LN   ++ E  D  +D
Sbjct: 725 KSLPKLLIDGPYGAPAQDYRKYDVLLLVGLGIGATPFISILKDLLNNIVKMEEHADSISD 784

Query: 80  KGRGS----------------LSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVE 139
             R S                L T+  Y YW  REQ SFDWFK  MN++   DQ +  +E
Sbjct: 785 FSRSSEYSTGSNGDTPRRKRILKTTNAYFYWVTREQGSFDWFKGVMNEVAELDQ-RGVIE 844

Query: 140 MHNFLTSIYHEGDVRSVLIRAIQALHYTRRGMDIVSKT 158
           MHN+LTS+Y EGD RS LI  +QAL++ + G+DIVS T
Sbjct: 845 MHNYLTSVYEEGDARSALITMVQALNHAKNGVDIVSGT 881

[Close]

BLAST of Carg15150 vs. ExPASy Swiss-Prot
Match: Q2HXK9 (Respiratory burst oxidase homolog protein D OS=Solanum tuberosum OX=4113 GN=RBOHD PE=1 SV=2)

HSP 1 Score: 142.9 bits (359), Expect = 3.1e-33
Identity = 68/142 (47.89%), Postives = 95/142 (66.90%), Query Frame = 0


Query: 22  YPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETCDGH-----TD 81
           YPK+ IDGPYGA +QD+ +Y  ++L+GLGIGATP +S++KD +N + E    H     T 
Sbjct: 655 YPKVLIDGPYGAPAQDYKEYEVLLLVGLGIGATPMISIVKDIVNNMKEEKYDHDLEKKTV 714

Query: 82  KGRGSLSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEGDVRS 141
            G G  +  ++Y YW  REQ SFDWFK  MN+L + D     +EMHN+ TS+Y EGD RS
Sbjct: 715 SGSGRSNFKRVYFYWVTREQGSFDWFKGLMNELAVMD-CDGIIEMHNYCTSVYEEGDARS 774

Query: 142 VLIRAIQALHYTRRGMDIVSKT 158
            LI  +Q++++ + G+DIVS T
Sbjct: 775 ALIAMLQSINHAKNGVDIVSGT 795

[Close]

BLAST of Carg15150 vs. ExPASy Swiss-Prot
Match: Q9SW17 (Putative respiratory burst oxidase homolog protein G OS=Arabidopsis thaliana OX=3702 GN=RBOHG PE=3 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 5.3e-33
Identity = 71/149 (47.65%), Postives = 102/149 (68.46%), Query Frame = 0


Query: 19  SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALN------RLSETCD 78
           S  +PKI IDGPYGA +QD+ KY  V+LIGLGIGATP +S++KD +N      +LS+   
Sbjct: 639 SPRFPKIMIDGPYGAPAQDYKKYEVVLLIGLGIGATPMISIIKDIINNTETKEQLSQMEK 698

Query: 79  GHTDKGRG---SLSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIY 138
           G   + +G   +  T + Y YW  +EQ +FDWFK+ MN++   D+++  +E+HN  TS+Y
Sbjct: 699 GSPQEQQGNKETFKTRRAYFYWVTKEQGTFDWFKNIMNEIAERDKSK-VIELHNHCTSVY 758

Query: 139 HEGDVRSVLIRAIQALHYTRRGMDIVSKT 158
            EGDVRS LIR +Q+L+Y + G+DIV+ T
Sbjct: 759 EEGDVRSALIRMLQSLNYAKNGLDIVAGT 786

[Close]

BLAST of Carg15150 vs. ExPASy Swiss-Prot
Match: Q948U0 (Respiratory burst oxidase homolog protein A OS=Solanum tuberosum OX=4113 GN=RBOHA PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 5.8e-32
Identity = 75/162 (46.30%), Postives = 101/162 (62.35%), Query Frame = 0


Query: 23  PKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNR---LSETCD------G 82
           PK+ IDGPYGA +QD+ KY+ ++L+GLGIGATPF+S+LKD L     + E  D      G
Sbjct: 740 PKLLIDGPYGAPAQDYRKYDVLLLVGLGIGATPFISILKDLLKNIVTMEEQADLVSDFSG 799

Query: 83  HTD-----------------KGRGSLSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQ 142
           ++D                 K + +L T+  Y YW  REQ SFDWFK  MN++   DQ +
Sbjct: 800 NSDMSAATSEQPALNKISPKKRKSTLKTTNAYFYWVTREQGSFDWFKGVMNEVAELDQ-R 859

Query: 143 SAVEMHNFLTSIYHEGDVRSVLIRAIQALHYTRRGMDIVSKT 158
             +EMHN+LTS+Y EGD RS LI  +QAL++ + G+DIVS T
Sbjct: 860 GVIEMHNYLTSVYEEGDARSALITMVQALNHAKNGVDIVSGT 900

[Close]

BLAST of Carg15150 vs. ExPASy Swiss-Prot
Match: Q948T9 (Respiratory burst oxidase homolog protein B OS=Solanum tuberosum OX=4113 GN=RBOHB PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 7.6e-32
Identity = 66/139 (47.48%), Postives = 95/139 (68.35%), Query Frame = 0


Query: 23  PKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETC---DGHTDKGR 82
           PK+ IDGPYGA +QD+ KY+ V+L+GLGIGATP +S++KD LN + +     DG     R
Sbjct: 667 PKLLIDGPYGAPAQDYKKYDVVLLVGLGIGATPLISIVKDVLNNIKQQKNIEDGTKGSKR 726

Query: 83  GSLSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEGDVRSVLI 142
              +T + Y YW  REQ SF+WFK  M++++ +DQ +  +E+HN+ TS+Y EGD RS LI
Sbjct: 727 SPFATKRAYFYWVTREQGSFEWFKGVMDEVSENDQ-EGLIELHNYCTSVYEEGDARSALI 786

Query: 143 RAIQALHYTRRGMDIVSKT 158
             +Q++   + G+DIVS T
Sbjct: 787 TMLQSIQQAKSGVDIVSGT 804

[Close]

BLAST of Carg15150 vs. ExPASy TrEMBL
Match: A0A6J1E0B7 (respiratory burst oxidase homolog protein F-like OS=Cucurbita moschata OX=3662 GN=LOC111429677 PE=4 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 7.5e-75
Identity = 142/145 (97.93%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 13  KGISLGSKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETCD 72
           KGISLGSKEYPKIFIDGPYGAASQD VKYNHVVLIGLGIGATPFMSVLKDALNRL+ETCD
Sbjct: 30  KGISLGSKEYPKIFIDGPYGAASQDLVKYNHVVLIGLGIGATPFMSVLKDALNRLTETCD 89

Query: 73  GHTDKGRGSLSTSKIYLYWAREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEGD 132
           GHTDKGRGSLSTSKIYLYWAREQSSFDWFKDFMNDL LSDQTQSAVEMHNFLTSIYHEGD
Sbjct: 90  GHTDKGRGSLSTSKIYLYWAREQSSFDWFKDFMNDLILSDQTQSAVEMHNFLTSIYHEGD 149

Query: 133 VRSVLIRAIQALHYTRRGMDIVSKT 158
           VRSVLIRAIQALHYTRRGMDIVSKT
Sbjct: 150 VRSVLIRAIQALHYTRRGMDIVSKT 174

[Close]

BLAST of Carg15150 vs. ExPASy TrEMBL
Match: A0A6J1I7P3 (respiratory burst oxidase homolog protein A-like OS=Cucurbita maxima OX=3661 GN=LOC111470370 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 3.0e-63
Identity = 122/140 (87.14%), Postives = 128/140 (91.43%), Query Frame = 0


Query: 19  SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETCDGHTDKG 78
           SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMS+LKD LNRL+  CDGHTD G
Sbjct: 586 SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSILKDVLNRLTGKCDGHTDNG 645

Query: 79  RGSLSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEGDVRSVL 138
           +GS S SK YLYW  REQSSFDWFKDFMNDLT SDQTQ AVEMHNFLTS++HE DVRSVL
Sbjct: 646 KGSPSKSKTYLYWVTREQSSFDWFKDFMNDLTQSDQTQLAVEMHNFLTSVHHENDVRSVL 705

Query: 139 IRAIQALHYTRRGMDIVSKT 158
           IRAIQALHYTRRG+DIVSKT
Sbjct: 706 IRAIQALHYTRRGIDIVSKT 725

[Close]

BLAST of Carg15150 vs. ExPASy TrEMBL
Match: A0A6J1EZJ2 (respiratory burst oxidase homolog protein A-like OS=Cucurbita moschata OX=3662 GN=LOC111440983 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 3.3e-62
Identity = 121/140 (86.43%), Postives = 127/140 (90.71%), Query Frame = 0


Query: 19  SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETCDGHTDKG 78
           SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMS+LKD LNRL+  CDGHTD G
Sbjct: 585 SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSILKDVLNRLTGKCDGHTDNG 644

Query: 79  RGSLSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEGDVRSVL 138
           +GS S SK YLYW  RE SSFDWFKDFMNDLT SDQTQ AVEMHNFLTS++HE DVRSVL
Sbjct: 645 KGSPSKSKTYLYWVTRELSSFDWFKDFMNDLTHSDQTQLAVEMHNFLTSVHHEDDVRSVL 704

Query: 139 IRAIQALHYTRRGMDIVSKT 158
           IRAIQALHYTRRG+DIVSKT
Sbjct: 705 IRAIQALHYTRRGIDIVSKT 724

[Close]

BLAST of Carg15150 vs. ExPASy TrEMBL
Match: A0A6J1BZ94 (respiratory burst oxidase homolog protein A-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111006961 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 7.3e-62
Identity = 120/146 (82.19%), Postives = 132/146 (90.41%), Query Frame = 0


Query: 13  KGISLGSKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETCD 72
           +G+S  SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLK  LNR +  CD
Sbjct: 579 EGMSPASKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKYVLNRFTGKCD 638

Query: 73  GHTDKGRGSLSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEG 132
           G+TD G GSLSTSK YLYW  REQSSF+WFKDFMNDLTLS++TQSAVEMHNFLT +Y EG
Sbjct: 639 GNTDNGEGSLSTSKTYLYWVTREQSSFNWFKDFMNDLTLSEKTQSAVEMHNFLTCVYQEG 698

Query: 133 DVRSVLIRAIQALHYTRRGMDIVSKT 158
           D+RSVLIRAIQALHYT+RG+DIVS+T
Sbjct: 699 DLRSVLIRAIQALHYTQRGIDIVSRT 724

[Close]

BLAST of Carg15150 vs. ExPASy TrEMBL
Match: A0A0A0LUN1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G013250 PE=3 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 2.4e-57
Identity = 112/140 (80.00%), Postives = 124/140 (88.57%), Query Frame = 0


Query: 19  SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRLSETCDGHTDKG 78
           SK YPKIFIDGPYGAASQDHVKYN+VVLIGLGIGATPFMS++KD + + +E  +   D G
Sbjct: 571 SKNYPKIFIDGPYGAASQDHVKYNNVVLIGLGIGATPFMSIMKDIVIKFTEKSNRRGDSG 630

Query: 79  RGSLSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIYHEGDVRSVL 138
           +G+ STSK YLYW  REQSSFDWFKDFMNDLT +DQTQ AVEMHNFLTS+YHEGDVRSVL
Sbjct: 631 KGNQSTSKTYLYWVTREQSSFDWFKDFMNDLTFTDQTQLAVEMHNFLTSVYHEGDVRSVL 690

Query: 139 IRAIQALHYTRRGMDIVSKT 158
           IRAIQALHYTRRG+DIVSKT
Sbjct: 691 IRAIQALHYTRRGIDIVSKT 710

[Close]

BLAST of Carg15150 vs. TAIR 10
Match: AT1G64060.1 (respiratory burst oxidase protein F )

HSP 1 Score: 146.0 bits (367), Expect = 2.6e-35
Identity = 77/158 (48.73%), Postives = 101/158 (63.92%), Query Frame = 0


Query: 20  KEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALN---RLSETCDGHTD 79
           K  PK+ IDGPYGA +QD+ KY+ ++L+GLGIGATPF+S+LKD LN   ++ E  D  +D
Sbjct: 725 KSLPKLLIDGPYGAPAQDYRKYDVLLLVGLGIGATPFISILKDLLNNIVKMEEHADSISD 784

Query: 80  KGRGS----------------LSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVE 139
             R S                L T+  Y YW  REQ SFDWFK  MN++   DQ +  +E
Sbjct: 785 FSRSSEYSTGSNGDTPRRKRILKTTNAYFYWVTREQGSFDWFKGVMNEVAELDQ-RGVIE 844

Query: 140 MHNFLTSIYHEGDVRSVLIRAIQALHYTRRGMDIVSKT 158
           MHN+LTS+Y EGD RS LI  +QAL++ + G+DIVS T
Sbjct: 845 MHNYLTSVYEEGDARSALITMVQALNHAKNGVDIVSGT 881

[Close]

BLAST of Carg15150 vs. TAIR 10
Match: AT4G25090.1 (Riboflavin synthase-like superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 3.7e-34
Identity = 71/149 (47.65%), Postives = 102/149 (68.46%), Query Frame = 0


Query: 19  SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALN------RLSETCD 78
           S  +PKI IDGPYGA +QD+ KY  V+LIGLGIGATP +S++KD +N      +LS+   
Sbjct: 639 SPRFPKIMIDGPYGAPAQDYKKYEVVLLIGLGIGATPMISIIKDIINNTETKEQLSQMEK 698

Query: 79  GHTDKGRG---SLSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIY 138
           G   + +G   +  T + Y YW  +EQ +FDWFK+ MN++   D+++  +E+HN  TS+Y
Sbjct: 699 GSPQEQQGNKETFKTRRAYFYWVTKEQGTFDWFKNIMNEIAERDKSK-VIELHNHCTSVY 758

Query: 139 HEGDVRSVLIRAIQALHYTRRGMDIVSKT 158
            EGDVRS LIR +Q+L+Y + G+DIV+ T
Sbjct: 759 EEGDVRSALIRMLQSLNYAKNGLDIVAGT 786

[Close]

BLAST of Carg15150 vs. TAIR 10
Match: AT4G25090.2 (Riboflavin synthase-like superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 3.7e-34
Identity = 71/149 (47.65%), Postives = 102/149 (68.46%), Query Frame = 0


Query: 19  SKEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALN------RLSETCD 78
           S  +PKI IDGPYGA +QD+ KY  V+LIGLGIGATP +S++KD +N      +LS+   
Sbjct: 627 SPRFPKIMIDGPYGAPAQDYKKYEVVLLIGLGIGATPMISIIKDIINNTETKEQLSQMEK 686

Query: 79  GHTDKGRG---SLSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIY 138
           G   + +G   +  T + Y YW  +EQ +FDWFK+ MN++   D+++  +E+HN  TS+Y
Sbjct: 687 GSPQEQQGNKETFKTRRAYFYWVTKEQGTFDWFKNIMNEIAERDKSK-VIELHNHCTSVY 746

Query: 139 HEGDVRSVLIRAIQALHYTRRGMDIVSKT 158
            EGDVRS LIR +Q+L+Y + G+DIV+ T
Sbjct: 747 EEGDVRSALIRMLQSLNYAKNGLDIVAGT 774

[Close]

BLAST of Carg15150 vs. TAIR 10
Match: AT4G11230.1 (Riboflavin synthase-like superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 5.4e-33
Identity = 72/168 (42.86%), Postives = 99/168 (58.93%), Query Frame = 0


Query: 20  KEYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALNRL------------ 79
           + +P++ IDGPYGA +QDH KY+ V+L+GLGIGATPF+S+L+D LN +            
Sbjct: 712 RSFPELLIDGPYGAPAQDHWKYDVVLLVGLGIGATPFVSILRDLLNNIIKQQEQAECISG 771

Query: 80  -----------------SETCDGHTDKGRGSLSTSKIYLYW-AREQSSFDWFKDFMNDLT 139
                            SE         R +L+T   Y YW  REQ SFDWFK+ MN++ 
Sbjct: 772 SCSNSNISSDHSFSCLNSEAASRIPQTQRKTLNTKNAYFYWVTREQGSFDWFKEIMNEIA 831

Query: 140 LSDQTQSAVEMHNFLTSIYHEGDVRSVLIRAIQALHYTRRGMDIVSKT 158
            SD+ +  +EMHN+LTS+Y EGD RS L+  IQ L++ + G+DI S T
Sbjct: 832 DSDR-KGVIEMHNYLTSVYEEGDTRSNLLTMIQTLNHAKNGVDIFSGT 878

[Close]

BLAST of Carg15150 vs. TAIR 10
Match: AT5G51060.1 (NADPH/respiratory burst oxidase protein D )

HSP 1 Score: 137.5 bits (345), Expect = 9.2e-33
Identity = 69/149 (46.31%), Postives = 99/149 (66.44%), Query Frame = 0


Query: 21  EYPKIFIDGPYGAASQDHVKYNHVVLIGLGIGATPFMSVLKDALN--------RLSETCD 80
           ++PK+ IDGPYGA +QD+ KY  V+L+GLGIGATP +S++KD +N        +L+   +
Sbjct: 695 DFPKVLIDGPYGAPAQDYKKYEVVLLVGLGIGATPMISIVKDIVNNIKAKEQAQLNRMEN 754

Query: 81  GHTDKGRG---SLSTSKIYLYW-AREQSSFDWFKDFMNDLTLSDQTQSAVEMHNFLTSIY 140
           G ++  R    S  T + Y YW  REQ SFDWFK+ MN++   D  +  +EMHN+ TS+Y
Sbjct: 755 GTSEPQRSKKESFRTRRAYFYWVTREQGSFDWFKNIMNEVAERDANR-VIEMHNYCTSVY 814

Query: 141 HEGDVRSVLIRAIQALHYTRRGMDIVSKT 158
            EGD RS LI  +Q+L++ + G+DIVS T
Sbjct: 815 EEGDARSALIHMLQSLNHAKNGVDIVSGT 842

The following BLAST results are available for this feature:

BLAST of Carg15150 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023296.1	4.8e-84	100.00	Respiratory burst oxidase-like protein F, partial [Cucurbita argyrosperma subsp....	[more]
KAG6589605.1	7.5e-77	100.00	Mitochondrial substrate carrier family protein E, partial [Cucurbita argyrosperm...	[more]
XP_022921387.1	1.6e-74	97.93	respiratory burst oxidase homolog protein F-like [Cucurbita moschata]	[more]
XP_022971705.1	6.1e-63	87.14	respiratory burst oxidase homolog protein A-like [Cucurbita maxima] >XP_02297171...	[more]
KAG6587580.1	1.0e-62	87.14	Respiratory burst oxidase-like protein A, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of Carg15150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48538	3.6e-34	48.73	Respiratory burst oxidase homolog protein F OS=Arabidopsis thaliana OX=3702 GN=R...	[more]
Q2HXK9	3.1e-33	47.89	Respiratory burst oxidase homolog protein D OS=Solanum tuberosum OX=4113 GN=RBOH...	[more]
Q9SW17	5.3e-33	47.65	Putative respiratory burst oxidase homolog protein G OS=Arabidopsis thaliana OX=...	[more]
Q948U0	5.8e-32	46.30	Respiratory burst oxidase homolog protein A OS=Solanum tuberosum OX=4113 GN=RBOH...	[more]
Q948T9	7.6e-32	47.48	Respiratory burst oxidase homolog protein B OS=Solanum tuberosum OX=4113 GN=RBOH...	[more]

BLAST of Carg15150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E0B7	7.5e-75	97.93	respiratory burst oxidase homolog protein F-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1I7P3	3.0e-63	87.14	respiratory burst oxidase homolog protein A-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1EZJ2	3.3e-62	86.43	respiratory burst oxidase homolog protein A-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1BZ94	7.3e-62	82.19	respiratory burst oxidase homolog protein A-like isoform X1 OS=Momordica charant...	[more]
A0A0A0LUN1	2.4e-57	80.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G013250 PE=3 SV=1	[more]

BLAST of Carg15150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64060.1	2.6e-35	48.73	respiratory burst oxidase protein F	[more]
AT4G25090.1	3.7e-34	47.65	Riboflavin synthase-like superfamily protein	[more]
AT4G25090.2	3.7e-34	47.65	Riboflavin synthase-like superfamily protein	[more]
AT4G11230.1	5.4e-33	42.86	Riboflavin synthase-like superfamily protein	[more]
AT5G51060.1	9.2e-33	46.31	NADPH/respiratory burst oxidase protein D	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000778	Cytochrome b245, heavy chain	PRINTS	PR00466	GP91PHOX	coord: 23..36 score: 64.29 coord: 46..63 score: 61.11
IPR013121	Ferric reductase, NAD binding domain	PFAM	PF08030	NAD_binding_6	coord: 41..157 e-value: 1.7E-22 score: 80.3
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	GENE3D	3.40.50.80		coord: 32..157 e-value: 1.3E-29 score: 105.2
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	SUPERFAMILY	52343	Ferredoxin reductase-like, C-terminal NADP-linked domain	coord: 24..111
None	No IPR available	PANTHER	PTHR11972:SF127	RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN A-LIKE	coord: 17..157
None	No IPR available	PANTHER	PTHR11972	NADPH OXIDASE	coord: 17..157
None	No IPR available	CDD	cd06186	NOX_Duox_like_FAD_NADP	coord: 16..128 e-value: 5.50082E-23 score: 88.1305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg15150-RA	Carg15150-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098869	cellular oxidant detoxification
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0050664	oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
molecular_function	GO:0004601	peroxidase activity
molecular_function	GO:0016491	oxidoreductase activity