Carg15084 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg15084
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	dihydrofolate synthetase isoform X1
Location	Carg_Chr16: 425754 .. 426785 (-)
RNA-Seq Expression	Carg15084
Synteny	Carg15084

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGAGGATTGTCGCAAGTCCAAGTGTTCCAACAGTTCTCGCTCAATCAGCTATTCTTCAAGCTTGCAAGTCGCATTTCCTACCATTTTTTTTCTCAAGAAAGAATGCCCATTTCACAATGAATCTCTTCAAATATCATCATCACTTCCGCCCGCAGATTCACGGAAGGCTTCTCCTAAATTATTTCGTTGGTGAAGGGCCTTCTATCAGTAGCCGAATTGGGTCCAAACAATGCTTCGGCAATCACTCAGAAGATCAAGACATGACGGAATTCATGGAATACTTGGATTCCCTGAAGAACTACGAGAAGTTGGGTGTGCCCACAGGCGCAGGGACGGAGTCTGAGGATGGGTTCGATCTCGGAAGGATGAAAAGACTGATGGAGCGCCTGGGTAATCCACAATCCAAGTTTAAGGTTGGTTATTTATGATGATCAACTCTTTACTTTTCTTAGTTTTCACTAAATTTTGAAGTTTTGGACCGACCCATTTCCTCTGCAATTCACTAAATTTAGTGTTTTATTCATATTAATTCTCTCCTCACTTGTGATGGATATCAATACCAATCGATGGGAAATGACACTACCAATGTTTGAACATTGCATATGAAAAGTTTAAGCTGTTAGTGACTGATGGTAGGACAATTATAGGCAATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCGGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGTTGTTATACTAGGTACAGCCTTGTTGCAACACTTTTCAAATTTTTCGTATACTCCTACTTGGAAGTGATTTGAGAAGGCATAACTTTGTGCTTGTGAATTTCTTCATGTGCAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGTAGATCTGGAGAGATGGTCTCTGGAAAGGCACTAAATTTTCTTTTCAAAAGGAACAAAGAGTTACTTGACCAATCGGTAAAACTTGAAAATGGACGTATAAGCCACTTCGAGGTATTATATCGTGCAGTCTGTTGTTGTGATTGA

mRNA sequence

GAGGAGGATTGTCGCAAGTCCAAGTGTTCCAACAGTTCTCGCTCAATCAGCTATTCTTCAAGCTTGCAAGTCGCATTTCCTACCATTTTTTTTCTCAAGAAAGAATGCCCATTTCACAATGAATCTCTTCAAATATCATCATCACTTCCGCCCGCAGATTCACGGAAGGCTTCTCCTAAATTATTTCGTTGGTGAAGGGCCTTCTATCAGTAGCCGAATTGGGTCCAAACAATGCTTCGGCAATCACTCAGAAGATCAAGACATGACGGAATTCATGGAATACTTGGATTCCCTGAAGAACTACGAGAAGTTGGGTGTGCCCACAGGCGCAGGGACGGAGTCTGAGGATGGGTTCGATCTCGGAAGGATGAAAAGACTGATGGAGCGCCTGGGTAATCCACAATCCAAGTTTAAGGCAATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCGGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGTTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGTAGATCTGGAGAGATGGTCTCTGGAAAGGCACTAAATTTTCTTTTCAAAAGGAACAAAGAGTTACTTGACCAATCGGTAAAACTTGAAAATGGACGTATAAGCCACTTCGAGGTATTATATCGTGCAGTCTGTTGTTGTGATTGA

Coding sequence (CDS)

ATGAATCTCTTCAAATATCATCATCACTTCCGCCCGCAGATTCACGGAAGGCTTCTCCTAAATTATTTCGTTGGTGAAGGGCCTTCTATCAGTAGCCGAATTGGGTCCAAACAATGCTTCGGCAATCACTCAGAAGATCAAGACATGACGGAATTCATGGAATACTTGGATTCCCTGAAGAACTACGAGAAGTTGGGTGTGCCCACAGGCGCAGGGACGGAGTCTGAGGATGGGTTCGATCTCGGAAGGATGAAAAGACTGATGGAGCGCCTGGGTAATCCACAATCCAAGTTTAAGGCAATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCGGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGTTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGTAGATCTGGAGAGATGGTCTCTGGAAAGGCACTAAATTTTCTTTTCAAAAGGAACAAAGAGTTACTTGACCAATCGGTAAAACTTGAAAATGGACGTATAAGCCACTTCGAGGTATTATATCGTGCAGTCTGTTGTTGTGATTGA

Protein sequence

MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISHFEVLYRAVCCCD

Homology

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BLAST of Carg15084 vs. NCBI nr
Match: KAG7014724.1 (Dihydrofolate synthetase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 395.6 bits (1015), Expect = 2.5e-106
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLYRAVCCCD 193
           FEVLYRAVCCCD
Sbjct: 181 FEVLYRAVCCCD 192

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BLAST of Carg15084 vs. NCBI nr
Match: KAG6576673.1 (Dihydrofolate synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 375.2 bits (962), Expect = 3.4e-100
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

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BLAST of Carg15084 vs. NCBI nr
Match: XP_022922898.1 (dihydrofolate synthetase isoform X1 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 6.5e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. NCBI nr
Match: XP_022922899.1 (dihydrofolate synthetase isoform X2 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 6.5e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. NCBI nr
Match: XP_022922900.1 (dihydrofolate synthetase isoform X3 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 6.5e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

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BLAST of Carg15084 vs. ExPASy Swiss-Prot
Match: F4JYE9 (Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 4.6e-47
Identity = 92/141 (65.25%), Postives = 115/141 (81.56%), Query Frame = 0


Query: 44  SEDQDMTEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHI 103
           +ED ++ +F+ +L+SLKNYEK GVP GAGT+S+DGFDLGRMKRLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KELLDQSVKLENGRISHFEVL 185
           K +L+QS++ ENG +SHFE+L
Sbjct: 160 KPILEQSIQEENGSLSHFEIL 178

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BLAST of Carg15084 vs. ExPASy Swiss-Prot
Match: Q05865 (Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=folC PE=3 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 7.6e-18
Identity = 46/104 (44.23%), Postives = 70/104 (67.31%), Query Frame = 0


Query: 81  LGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 140
           LGRMK+LM RLG+P+ K +A H+AGT GKGST AF+ ++L+  GY+VG +TSP+I T  E
Sbjct: 24  LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNE 83

Query: 141 RISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISHFEVL 185
           RIS+  +G  +S +    L  + K  ++   + E G+ + FE++
Sbjct: 84  RISV--NGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIM 125

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BLAST of Carg15084 vs. ExPASy Swiss-Prot
Match: Q12676 (Dihydrofolate synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=FOL3 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.1e-11
Identity = 27/64 (42.19%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 81  LGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 140
           L R+ +L+E LGNPQ+  + +HIAGT GKGS   +LS++L+ + Y +G +T+PH+  + +
Sbjct: 7   LSRITKLLEHLGNPQNSLRVLHIAGTNGKGSVCTYLSSVLQQKSYQIGKFTTPHLVHVTD 66

Query: 141 RISL 145
            I++
Sbjct: 67  SITI 70

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BLAST of Carg15084 vs. ExPASy Swiss-Prot
Match: Q54CY5 (Putative folylpolyglutamate synthase OS=Dictyostelium discoideum OX=44689 GN=folC PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.5e-10
Identity = 39/104 (37.50%), Postives = 58/104 (55.77%), Query Frame = 0


Query: 74  ESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSP 133
           E    F +  M+   + L     +   IH+AGTKGKGST A   +I+R +G+S G +TSP
Sbjct: 110 EESAKFLMEEMRNYCKTLSIDLERESIIHVAGTKGKGSTCAITESIIREQGFSTGLFTSP 169

Query: 134 HIETIRERISLGRSGEMVSGKALNFLFKRNKELL--DQSVKLEN 176
           H+ + RERI +  +GEM+S +  +  F    +LL  D   +L N
Sbjct: 170 HLISPRERIRI--NGEMISKEMFSQYFWNCWDLLIKDYQTQLPN 211

[Close]

BLAST of Carg15084 vs. ExPASy Swiss-Prot
Match: P15925 (Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.7e-09
Identity = 36/95 (37.89%), Postives = 49/95 (51.58%), Query Frame = 0


Query: 50  TEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGK 109
           TE + Y+ S     K G             D  R+  L+  LGNPQ + + IH+ GT GK
Sbjct: 4   TETVAYIHSFPRLAKTG-------------DHRRILTLLHALGNPQQQGRYIHVTGTNGK 63

Query: 110 GSTAAFLSNILRAEGYSVGCYTSPHIETIRERISL 145
           GS A  ++++L A G +VG YTSP I    ERI +
Sbjct: 64  GSAANAIAHVLEASGLTVGLYTSPFIMRFNERIMI 85

[Close]

BLAST of Carg15084 vs. ExPASy TrEMBL
Match: A0A6J1E4S6 (dihydrofolate synthetase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430739 PE=3 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.1e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. ExPASy TrEMBL
Match: A0A6J1EA44 (dihydrofolate synthetase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111430739 PE=3 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.1e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. ExPASy TrEMBL
Match: A0A6J1E819 (dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430739 PE=3 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.1e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. ExPASy TrEMBL
Match: A0A6J1E5E1 (dihydrofolate synthetase isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111430739 PE=3 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.1e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. ExPASy TrEMBL
Match: A0A6J1J6Z7 (dihydrofolate synthetase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483133 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.1e-96
Identity = 179/184 (97.28%), Postives = 181/184 (98.37%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MN+FKYHHHFR QI GRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQ MTEFMEYLDSLK
Sbjct: 1   MNVFKYHHHFRLQILGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. TAIR 10
Match: AT5G41480.1 (Folylpolyglutamate synthetase family protein )

HSP 1 Score: 189.1 bits (479), Expect = 3.3e-48
Identity = 92/141 (65.25%), Postives = 115/141 (81.56%), Query Frame = 0


Query: 44  SEDQDMTEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHI 103
           +ED ++ +F+ +L+SLKNYEK GVP GAGT+S+DGFDLGRMKRLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KELLDQSVKLENGRISHFEVL 185
           K +L+QS++ ENG +SHFE+L
Sbjct: 160 KPILEQSIQEENGSLSHFEIL 178

[Close]

BLAST of Carg15084 vs. TAIR 10
Match: AT3G55630.1 (DHFS-FPGS homolog D )

HSP 1 Score: 62.8 bits (151), Expect = 3.5e-10
Identity = 39/104 (37.50%), Postives = 52/104 (50.00%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L    E+   K 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL-NGIEISQEKF 115

Query: 156 LNFL---FKRNKELLDQSVKLE------NGRISHFEVLYRAVCC 191
           +N+    F + KE     V +        GR     V+ + V C
Sbjct: 116 VNYFWCCFHKLKEKTSNEVDVVILEVGLGGRFDATNVIQKPVVC 158

[Close]

BLAST of Carg15084 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 60.1 bits (144), Expect = 2.3e-09
Identity = 34/81 (41.98%), Postives = 45/81 (55.56%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L    E+   K 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL-NGIEISQEKF 115

Query: 156 LNFL---FKRNKELLDQSVKL 174
           +N+    F + KE     V +
Sbjct: 116 VNYFWCCFHKLKEKTSNEVPM 135

[Close]

BLAST of Carg15084 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 60.1 bits (144), Expect = 2.3e-09
Identity = 34/81 (41.98%), Postives = 45/81 (55.56%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L    E+   K 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL-NGIEISQEKF 115

Query: 156 LNFL---FKRNKELLDQSVKL 174
           +N+    F + KE     V +
Sbjct: 116 VNYFWCCFHKLKEKTSNEVPM 135

[Close]

BLAST of Carg15084 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 59.7 bits (143), Expect = 3.0e-09
Identity = 35/94 (37.23%), Postives = 47/94 (50.00%), Query Frame = 0


Query: 51  EFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKG 110
           E +  L SL         +  G   E  FD  ++  L E +     K   IH+AGTKGKG
Sbjct: 11  EALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDI----LKMNVIHVAGTKGKG 70

Query: 111 STAAFLSNILRAEGYSVGCYTSPHIETIRERISL 145
           ST  F  +I+R  G+  G +TSPH+  +RER  L
Sbjct: 71  STCTFTESIIRNYGFRTGLFTSPHLIDVRERFRL 100

The following BLAST results are available for this feature:

BLAST of Carg15084 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014724.1	2.5e-106	100.00	Dihydrofolate synthetase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6576673.1	3.4e-100	100.00	Dihydrofolate synthetase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022922898.1	6.5e-99	98.91	dihydrofolate synthetase isoform X1 [Cucurbita moschata]	[more]
XP_022922899.1	6.5e-99	98.91	dihydrofolate synthetase isoform X2 [Cucurbita moschata]	[more]
XP_022922900.1	6.5e-99	98.91	dihydrofolate synthetase isoform X3 [Cucurbita moschata]	[more]

BLAST of Carg15084 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JYE9	4.6e-47	65.25	Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1	[more]
Q05865	7.6e-18	44.23	Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain ...	[more]
Q12676	3.1e-11	42.19	Dihydrofolate synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	[more]
Q54CY5	4.5e-10	37.50	Putative folylpolyglutamate synthase OS=Dictyostelium discoideum OX=44689 GN=fol...	[more]
P15925	1.7e-09	37.89	Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1	[more]

BLAST of Carg15084 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E4S6	3.1e-99	98.91	dihydrofolate synthetase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11143073...	[more]
A0A6J1EA44	3.1e-99	98.91	dihydrofolate synthetase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11143073...	[more]
A0A6J1E819	3.1e-99	98.91	dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11143073...	[more]
A0A6J1E5E1	3.1e-99	98.91	dihydrofolate synthetase isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC11143073...	[more]
A0A6J1J6Z7	1.1e-96	97.28	dihydrofolate synthetase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483133 ...	[more]

BLAST of Carg15084 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41480.1	3.3e-48	65.25	Folylpolyglutamate synthetase family protein	[more]
AT3G55630.1	3.5e-10	37.50	DHFS-FPGS homolog D	[more]
AT3G55630.3	2.3e-09	41.98	DHFS-FPGS homolog D	[more]
AT3G55630.2	2.3e-09	41.98	DHFS-FPGS homolog D	[more]
AT5G05980.2	3.0e-09	37.23	DHFS-FPGS homolog B	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	3.40.1190.10		coord: 38..190 e-value: 2.0E-36 score: 127.5
IPR036565	Mur-like, catalytic domain superfamily	SUPERFAMILY	53623	MurD-like peptide ligases, catalytic domain	coord: 77..186
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 46..184
None	No IPR available	PANTHER	PTHR11136:SF0	DIHYDROFOLATE SYNTHETASE-RELATED	coord: 46..184
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01011	FOLYLPOLYGLU_SYNT_1	coord: 101..124

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg15084-RA	Carg15084-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006761	dihydrofolate biosynthetic process
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0046901	tetrahydrofolylpolyglutamate biosynthetic process
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005759	mitochondrial matrix
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0008841	dihydrofolate synthase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004326	tetrahydrofolylpolyglutamate synthase activity