Carg14689 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14689
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionCBS domain-containing protein CBSX5-like
LocationCarg_Chr01: 9751913 .. 9753588 (+)
RNA-Seq ExpressionCarg14689
SyntenyCarg14689
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTATTCTTCATCTTCTCCATCTTCTCCCATTTCTCCGATCTTCCGCCGTTGCCGGATTCTGACGACCTCCTTTCCTCTCCGATAACCGTTCCTCCTCCCTTTTAGCTTTTGTATGGCAGTGAGCTTGTTTTCGCATGACGTTTCTGATCTCTGTCTCGGCAAGCCAGCGCTGAGGCTGTTGTCTTTGTCCGCAACTGTCGCCGACGCCTTGACTGCTCTCAAATTCTCCGACGATTACTTCGTCAGCGTTTGGGACTGCCGTTTGACGAAGAACGGTTGCGGTGGCGGTGATGTTGATGGTAGTGCTGACGGTGGTGAGTTTGAGTGCTGCCGATGCGTGGGGAAGCTGTGTATGGTGGATGTGATTTGCTATCTTTGTAGGGACGAGAATTTGTTGTCGCCTTCGGCTGCATTGTGTGCTCCTATCTCTGAGATTTTGCCACAAATTCCTGGAATCGTGATGCATTTGGAGCCGTCTGCTAGGTATAGAAATGAATCTGATGAAATTTGGATCTATATTTTTCCTTTCTTAGGATTGTTTCAATTTATTCTTTAATTAGTTCGTTTCTGAATGATTGTTAATGGCTGCGTTTTATTGATGTTTCAGTTTGAGTTTGATATTGATATTCTTCAATTAGGTTTCTGAAGTTTTGTCAGTTACTGATCTGAAATGAGCGTTACAGAACTTTTTTTTCAAACTCAGCTTTGAAATGTTTGTTTCTTTCCTTAGCTCTTCTACTTTTACTCTCAGACTTTATATGATTCTGAACTTGCAAAGAGAATATTCCAGCTTGTTAGATGAAGATAACTGAAGTCTCTGTGTTGATATTCCAGCTTGTTAGATGCCATTGATCTGGTTCTCCAAGGTGCTCAAAATCTTGTAGTCCCAATCAAGGCAAAGCTAGGAAGCAATTCAAGAAGAAAGCAGCTTAAAACCCCCACAAATGCCATCTATGGCGGCCGTGAATTCTGCTGGCTGACTCAGGAAGATATAATCAGATATCTCCTCAGCTCAATTGGCCATTTTTCCCCCATTGCTGCCCTCTCCCTTGACAACCTTGGCATCATCTGCACCAATCCCTTATCAGTAAACTACCACTCCCCTGCCTCCTCTGCCATTGGAGCCATATCCCACTGCATTCGCCAATCAAACCTCGGTAGCAGTCGTCGACGCCGATGGAATTCTGATTGGTGAGATCTCACCCTTTGCCCTTGCCAGGTGTGACAAGGCTGTTGCAGCTGCGATTATGACATTGTCTTCTGGAGATCTGATGGCCTACATTGACTGTGGAGGGCCTCCAGAGGATCTTGTTAAGGTAGTGAAGGCTAGGCTGAAAGAGAACAACTTGGAAGGAATGCTGCAAGAATTCACTAATTCACCATCCTCAATTGGATCTCCATCTTTCACTTCTTCATCTTCTGATGAGGAGTTCTCCCCATCTCCGAGCTCAAGAAAATACAGAAGATCATCAAGTTACTCAGCACGAATCACTCGTCGTTCCGAGGCCATCGTTTGTCATCCAAGGAGCTCCCTGATAGCTGTTATCATCCAGGCAATCGCGCACCGTGTGAACTACGTCTGGGTTATCGAAGACGACTGCAGTTTGATCGGAATCGTCACATTCCTTGATATGTTAAAAGTTTTCAGAGAACATTTAGAGACAAGTGAGTAG

mRNA sequence

CTTTTATTCTTCATCTTCTCCATCTTCTCCCATTTCTCCGATCTTCCGCCGTTGCCGGATTCTGACGACCTCCTTTCCTCTCCGATAACCGTTCCTCCTCCCTTTTAGCTTTTGTATGGCAGTGAGCTTGTTTTCGCATGACGTTTCTGATCTCTGTCTCGGCAAGCCAGCGCTGAGGCTGTTGTCTTTGTCCGCAACTGTCGCCGACGCCTTGACTGCTCTCAAATTCTCCGACGATTACTTCGTCAGCGTTTGGGACTGCCGTTTGACGAAGAACGGTTGCGGTGGCGGTGATGTTGATGGTAGTGCTGACGGTGGTGAGTTTGAGTGCTGCCGATGCGTGGGGAAGCTGTGTATGGTGGATGTGATTTGCTATCTTTGTAGGGACGAGAATTTGTTGTCGCCTTCGGCTGCATTGTGTGCTCCTATCTCTGAGATTTTGCCACAAATTCCTGGAATCGTGATGCATTTGGAGCCGTCTGCTAGCTTGTTAGATGCCATTGATCTGGTTCTCCAAGGTGCTCAAAATCTTGTAGTCCCAATCAAGGCAAAGCTAGGAAGCAATTCAAGAAGAAAGCAGCTTAAAACCCCCACAAATGCCATCTATGGCGGCCGTGAATTCTGCTGGCTGACTCAGGAAGATATAATCAGATATCTCCTCAGCTCAATTGGCCATTTTTCCCCCATTGCTGCCCTCTCCCTTGACAACCTTGGCATCATCTGCACCAATCCCTTATCAGTAAACTACCACTCCCCTGCCTCCTCTGCCATTGGAGCCATATCCCACTGCATTCGCCAATCAAACCTCGTCGTCGACGCCGATGGAATTCTGATTGGTGAGATCTCACCCTTTGCCCTTGCCAGGTGTGACAAGGCTGTTGCAGCTGCGATTATGACATTGTCTTCTGGAGATCTGATGGCCTACATTGACTGTGGAGGGCCTCCAGAGGATCTTGTTAAGGTAGTGAAGGCTAGGCTGAAAGAGAACAACTTGGAAGGAATGCTGCAAGAATTCACTAATTCACCATCCTCAATTGGATCTCCATCTTTCACTTCTTCATCTTCTGATGAGGAGTTCTCCCCATCTCCGAGCTCAAGAAAATACAGAAGATCATCAAGTTACTCAGCACGAATCACTCGTCGTTCCGAGGCCATCGTTTGTCATCCAAGGAGCTCCCTGATAGCTGTTATCATCCAGGCAATCGCGCACCGTGTGAACTACGTCTGGGTTATCGAAGACGACTGCAGTTTGATCGGAATCGTCACATTCCTTGATATGTTAAAAGTTTTCAGAGAACATTTAGAGACAAGTGAGTAG

Coding sequence (CDS)

ATGGCAGTGAGCTTGTTTTCGCATGACGTTTCTGATCTCTGTCTCGGCAAGCCAGCGCTGAGGCTGTTGTCTTTGTCCGCAACTGTCGCCGACGCCTTGACTGCTCTCAAATTCTCCGACGATTACTTCGTCAGCGTTTGGGACTGCCGTTTGACGAAGAACGGTTGCGGTGGCGGTGATGTTGATGGTAGTGCTGACGGTGGTGAGTTTGAGTGCTGCCGATGCGTGGGGAAGCTGTGTATGGTGGATGTGATTTGCTATCTTTGTAGGGACGAGAATTTGTTGTCGCCTTCGGCTGCATTGTGTGCTCCTATCTCTGAGATTTTGCCACAAATTCCTGGAATCGTGATGCATTTGGAGCCGTCTGCTAGCTTGTTAGATGCCATTGATCTGGTTCTCCAAGGTGCTCAAAATCTTGTAGTCCCAATCAAGGCAAAGCTAGGAAGCAATTCAAGAAGAAAGCAGCTTAAAACCCCCACAAATGCCATCTATGGCGGCCGTGAATTCTGCTGGCTGACTCAGGAAGATATAATCAGATATCTCCTCAGCTCAATTGGCCATTTTTCCCCCATTGCTGCCCTCTCCCTTGACAACCTTGGCATCATCTGCACCAATCCCTTATCAGTAAACTACCACTCCCCTGCCTCCTCTGCCATTGGAGCCATATCCCACTGCATTCGCCAATCAAACCTCGTCGTCGACGCCGATGGAATTCTGATTGGTGAGATCTCACCCTTTGCCCTTGCCAGGTGTGACAAGGCTGTTGCAGCTGCGATTATGACATTGTCTTCTGGAGATCTGATGGCCTACATTGACTGTGGAGGGCCTCCAGAGGATCTTGTTAAGGTAGTGAAGGCTAGGCTGAAAGAGAACAACTTGGAAGGAATGCTGCAAGAATTCACTAATTCACCATCCTCAATTGGATCTCCATCTTTCACTTCTTCATCTTCTGATGAGGAGTTCTCCCCATCTCCGAGCTCAAGAAAATACAGAAGATCATCAAGTTACTCAGCACGAATCACTCGTCGTTCCGAGGCCATCGTTTGTCATCCAAGGAGCTCCCTGATAGCTGTTATCATCCAGGCAATCGCGCACCGTGTGAACTACGTCTGGGTTATCGAAGACGACTGCAGTTTGATCGGAATCGTCACATTCCTTGATATGTTAAAAGTTTTCAGAGAACATTTAGAGACAAGTGAGTAG

Protein sequence

MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGDVDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLEPSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRYLLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCIRQSNLVVDADGILIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQEFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAVIIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE
Homology
BLAST of Carg14689 vs. NCBI nr
Match: XP_022940315.1 (CBS domain-containing protein CBSX5-like [Cucurbita moschata])

HSP 1 Score: 763.5 bits (1970), Expect = 9.4e-217
Identity = 393/402 (97.76%), Postives = 396/402 (98.51%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60
           MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD
Sbjct: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60

Query: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120
           VDGSADGGEFECCRCVGKLCMVDVICYLCRD+NLLSPSAALCAPISEILPQIPGIVMHLE
Sbjct: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDQNLLSPSAALCAPISEILPQIPGIVMHLE 120

Query: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRY 180
           PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTP NAI+GGREFCWLTQEDIIRY
Sbjct: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPKNAIHGGREFCWLTQEDIIRY 180

Query: 181 LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCI--RQSNLVVDADGI 240
           LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCI  + S  VVDADGI
Sbjct: 181 LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCIANQTSVAVVDADGI 240

Query: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300
           LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ
Sbjct: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300

Query: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360
           EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV
Sbjct: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360

Query: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 401
           IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE
Sbjct: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 402

BLAST of Carg14689 vs. NCBI nr
Match: XP_023525010.1 (CBS domain-containing protein CBSX5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 761.5 bits (1965), Expect = 3.6e-216
Identity = 393/402 (97.76%), Postives = 394/402 (98.01%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60
           MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSD YFVSVWDCRLTKNGCGGGD
Sbjct: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDGYFVSVWDCRLTKNGCGGGD 60

Query: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120
           VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE
Sbjct: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120

Query: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRY 180
           PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRY
Sbjct: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRY 180

Query: 181 LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCI--RQSNLVVDADGI 240
           LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAIS CI  + S  VVDADGI
Sbjct: 181 LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISRCIANQTSVAVVDADGI 240

Query: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300
           LIGEISPF LARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ
Sbjct: 241 LIGEISPFTLARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300

Query: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360
           EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV
Sbjct: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360

Query: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 401
           IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE
Sbjct: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 402

BLAST of Carg14689 vs. NCBI nr
Match: XP_022981296.1 (CBS domain-containing protein CBSX5-like [Cucurbita maxima])

HSP 1 Score: 755.4 bits (1949), Expect = 2.6e-214
Identity = 389/402 (96.77%), Postives = 393/402 (97.76%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60
           MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRL KNGCGGGD
Sbjct: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLAKNGCGGGD 60

Query: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120
           VDGSADGG+FECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE
Sbjct: 61  VDGSADGGDFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120

Query: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRY 180
           PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLK PTNAI+GGREFCWLTQEDIIRY
Sbjct: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKNPTNAIHGGREFCWLTQEDIIRY 180

Query: 181 LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCI--RQSNLVVDADGI 240
           LLSSIGHFSPIAALSLDNLGIICTN LS+NYHSPASSAIGAIS CI  + S  VVDADGI
Sbjct: 181 LLSSIGHFSPIAALSLDNLGIICTNALSINYHSPASSAIGAISRCIANQTSVAVVDADGI 240

Query: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300
           LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ
Sbjct: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300

Query: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360
           EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV
Sbjct: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360

Query: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 401
           IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE
Sbjct: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 402

BLAST of Carg14689 vs. NCBI nr
Match: XP_038898999.1 (CBS domain-containing protein CBSX5 [Benincasa hispida])

HSP 1 Score: 680.2 bits (1754), Expect = 1.0e-191
Identity = 352/402 (87.56%), Postives = 375/402 (93.28%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60
           MAVSLFSHDVSDLCLGKPALR LSLSA VADAL AL+FSDDYFVSVWDCRL K+GC  G+
Sbjct: 1   MAVSLFSHDVSDLCLGKPALRPLSLSAAVADALVALQFSDDYFVSVWDCRLAKSGC-SGE 60

Query: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120
            DG A GG+F+CCRCVGKLCMVDVICYLC+DENLLSPSAAL A +SEILPQIPGIVMHLE
Sbjct: 61  GDGGAGGGDFDCCRCVGKLCMVDVICYLCKDENLLSPSAALRASVSEILPQIPGIVMHLE 120

Query: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRY 180
           P+ASLL+AIDLVLQGAQNLVVPIK KLGSNSRRKQLK PTNAI+GG EFCWLTQEDIIRY
Sbjct: 121 PTASLLEAIDLVLQGAQNLVVPIKTKLGSNSRRKQLKNPTNAIHGGLEFCWLTQEDIIRY 180

Query: 181 LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCI--RQSNLVVDADGI 240
           LLSSIG FS IAALSLD+LGIICTNPLSVNYHSPASSAIGAIS CI  + S  V+D +GI
Sbjct: 181 LLSSIGLFSSIAALSLDSLGIICTNPLSVNYHSPASSAIGAISRCIANQTSVAVIDGEGI 240

Query: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300
           LIGEISPFALA CDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLK++ LEGML+
Sbjct: 241 LIGEISPFALAGCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKDSKLEGMLE 300

Query: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360
           EFTNSPSSIGS SFTSSSSDEEFSPSPSSR+YRRSSSYSARITRR+EAIVCHPRSSL+AV
Sbjct: 301 EFTNSPSSIGSVSFTSSSSDEEFSPSPSSRRYRRSSSYSARITRRAEAIVCHPRSSLVAV 360

Query: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 401
           +IQAIAHRVNYVWVIEDDCSLIG+VTFLDMLKVFREHLE +E
Sbjct: 361 MIQAIAHRVNYVWVIEDDCSLIGMVTFLDMLKVFREHLEITE 401

BLAST of Carg14689 vs. NCBI nr
Match: XP_004141365.1 (CBS domain-containing protein CBSX5 [Cucumis sativus] >KGN55142.1 hypothetical protein Csa_012312 [Cucumis sativus])

HSP 1 Score: 674.9 bits (1740), Expect = 4.4e-190
Identity = 350/402 (87.06%), Postives = 371/402 (92.29%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60
           MAVSLFSHDVSDLCLGKP LR LSLSAT+ADAL AL+FS DYFVSVWDCRL K GC G  
Sbjct: 1   MAVSLFSHDVSDLCLGKPPLRPLSLSATLADALLALQFSHDYFVSVWDCRLPKRGCTGA- 60

Query: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120
           VDG A GG+FECCRCVGKLCMVDVICYLC++ENLLSPS+AL A +SEILPQIPGIVMHLE
Sbjct: 61  VDGGAAGGDFECCRCVGKLCMVDVICYLCKEENLLSPSSALQASVSEILPQIPGIVMHLE 120

Query: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRY 180
           PSASLL+AIDLVLQGAQNLVVPIK +LGSNSRRKQLK  TN I+GG EFCWLTQEDIIRY
Sbjct: 121 PSASLLEAIDLVLQGAQNLVVPIKTRLGSNSRRKQLKNSTNGIHGGHEFCWLTQEDIIRY 180

Query: 181 LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCI--RQSNLVVDADGI 240
           LL SIG FSPIAALSLD+LGIICTN LSVNYHSPASSAIGAISH I  + S  V+D DGI
Sbjct: 181 LLGSIGLFSPIAALSLDSLGIICTNALSVNYHSPASSAIGAISHSITNQTSVAVIDGDGI 240

Query: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300
           LIGEISPFALA CDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLK++ LEGML+
Sbjct: 241 LIGEISPFALAGCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKDSKLEGMLE 300

Query: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360
           EFTNSPSSIGSPSFTSSSSDEEFSPSPSSR+YRRSSSYSARITRR+EAIVCHPRSSL+AV
Sbjct: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRRYRRSSSYSARITRRAEAIVCHPRSSLVAV 360

Query: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 401
           +IQAI HRVNYVWVIEDDCSLIG+VTFLDMLKVFREHLETSE
Sbjct: 361 MIQAITHRVNYVWVIEDDCSLIGMVTFLDMLKVFREHLETSE 401

BLAST of Carg14689 vs. ExPASy Swiss-Prot
Match: Q84WQ5 (CBS domain-containing protein CBSX5 OS=Arabidopsis thaliana OX=3702 GN=CBSX5 PE=2 SV=2)

HSP 1 Score: 330.5 bits (846), Expect = 2.7e-89
Identity = 194/409 (47.43%), Postives = 275/409 (67.24%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLL-SLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGG 60
           MA+SL S++VSDLCLGKP LR L S S++V+DA+ ALK S+D F+SVW+C          
Sbjct: 1   MALSLLSYNVSDLCLGKPPLRCLSSSSSSVSDAIAALKSSEDTFLSVWNC--------NH 60

Query: 61  DVDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHL 120
           D D + +      C C+GK+ M DVIC+L +D +      AL + +S +LP+   IV+H+
Sbjct: 61  DDDNNTE------CECLGKISMADVICHLSKDHD--HSLCALNSSVSVLLPKTRSIVLHV 120

Query: 121 EPSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRR--KQLKTPTNAIYGGREFCWLTQEDI 180
           +PS SL++AIDL+++GAQNL+VPI  K  +  ++    +   T     G+ FCW+TQEDI
Sbjct: 121 QPSCSLIEAIDLIIKGAQNLIVPIHTKPYTKKKQHNDNVSVTTTTHSNGQRFCWITQEDI 180

Query: 181 IRYLLSSIGHFSPIAALSLDNLGIICT--NPLSVNYHSPASSAIGAISH--CIRQSNLVV 240
           I++LL  I  FSP+ A+SL +LG+I +    ++V+YHS AS+ + A+S+   ++ S  VV
Sbjct: 181 IQFLLGFIAAFSPLPAMSLSDLGVINSTHTVVAVDYHSSASAVVSAVSNALAVQTSVAVV 240

Query: 241 DADG-----ILIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARL 300
           D +G      LIGEISP  L  CD+  AAA+ TLS+GDLMAYID   PPE LV++V+ RL
Sbjct: 241 DGEGDDPFTSLIGEISPMTLTCCDETAAAAVATLSAGDLMAYIDGANPPESLVQIVRNRL 300

Query: 301 KENNLEGMLQEFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIV 360
           ++  L G++  F     S+ S S +S  S EE +P  ++  Y RS S SAR+ R+SEAIV
Sbjct: 301 EDKGLIGLMSLF----DSLSSYSTSSGYSSEEEAPVRTT-SYGRSMSSSARMARKSEAIV 360

Query: 361 CHPRSSLIAVIIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLE 398
           C+P+SSL+AV+IQA+AHRVNY WV+E D   +G+VTF+D+LKVFR+ LE
Sbjct: 361 CNPKSSLMAVMIQAVAHRVNYAWVVEKDGCFVGMVTFVDILKVFRKFLE 388

BLAST of Carg14689 vs. ExPASy Swiss-Prot
Match: Q8GZA4 (CBS domain-containing protein CBSX6 OS=Arabidopsis thaliana OX=3702 GN=CBSX6 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 7.4e-23
Identity = 118/428 (27.57%), Postives = 189/428 (44.16%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60
           MA     H V DL +GKP +     + TV  A+ A+  S +  + VW  R T +      
Sbjct: 1   MASVFLYHVVGDLTVGKPEMVEFYETETVESAIRAIGESTECGIPVWRKRTTPS------ 60

Query: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120
           + G  +  E    R VG L  +D++ +L + E  L    A+  P+SE++     ++  ++
Sbjct: 61  LPGFVENSEMRQQRFVGILNSLDIVAFLAKTE-CLQEEKAMKIPVSEVVSPDNTLLKQVD 120

Query: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRR-------KQLKT--------------- 180
           P   L+DA++++ QG + L+VP        S+R       K LK                
Sbjct: 121 PGTRLIDALEMMKQGVRRLLVPKSVVWRGMSKRFSILYNGKWLKNSENSSSSSGLSADST 180

Query: 181 --PTNAIYGGRE-FCWLTQEDIIRYLLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPA 240
             PT ++   R+ FC L++ED+IR+L+  +G  +P+   S+  LGII  N   +    PA
Sbjct: 181 NRPTTSMTSSRDKFCCLSREDVIRFLIGVLGALAPLPLTSISTLGIINQNYNFIEASLPA 240

Query: 241 SSAI-------GAISHCIRQSNLVVDADGILIGEISPFALARCDKAVAA-AIMTLSSGDL 300
             A         AI+   +  N   +    +IGEIS   L +CD   AA A+  L +G  
Sbjct: 241 IEATRRPLCDPSAIAVLEQTEN---EQQFKIIGEISASKLWKCDYLAAAWALANLYAG-- 300

Query: 301 MAYIDCGGPPEDLVKVVKARLKENNLEGMLQEFTNSPSSIGSPSFTS----SSSDEEFSP 360
                        V  V+  +   +    LQ  T+ P    + + T+    SS    F+P
Sbjct: 301 -----------QFVMGVEDNMSSRSFSDFLQ--TSFPGGEQNGTATNAKKFSSRSIGFNP 360

Query: 361 SPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAVIIQAIAHRVNYVWVIEDDCS--LIG 390
           +  +R     S Y      RS  + C   SSL AV+ Q ++HR  +VWV E D    L+G
Sbjct: 361 TSPTRLSIGRSMYRG----RSAPLTCKTSSSLAAVMAQMLSHRATHVWVTEADSDDVLVG 399

BLAST of Carg14689 vs. ExPASy Swiss-Prot
Match: Q8GXI9 (SNF1-related protein kinase regulatory subunit gamma-like PV42b OS=Arabidopsis thaliana OX=3702 GN=PV42B PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 3.3e-07
Identity = 88/394 (22.34%), Postives = 161/394 (40.86%), Query Frame = 0

Query: 5   LFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGDVDGS 64
           L    V DL + K  L  +  +AT+ DAL  +  +    V V        G GG  +   
Sbjct: 7   LMQFKVKDLMIDKRRLVEVPDNATLGDALNTMVANRVRAVPVAAKPGQWLGAGGSMIVEL 66

Query: 65  ADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIP-GI-VMHLEPS 124
                    + +G + M+DV+ ++  D+        + AP+S I+   P G+ +  L P+
Sbjct: 67  DKQSGSARKQYIGMVTMLDVVAHIAGDDGESGLDKKMAAPVSSIIGHCPEGLSLWSLNPN 126

Query: 125 ASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRYLL 184
            S++D ++++ +G   ++VP    L SN+      T    +     +  L+Q D+I +  
Sbjct: 127 TSIMDCMEMLSKGIHRVLVP----LDSNTEN---ITGPELVESASAYAMLSQMDLISFFF 186

Query: 185 SSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCIRQSNLVVDADGILIGE 244
                   I + ++ +L  I    L++   +    AI  +S  +  +  +V+A G   GE
Sbjct: 187 DQSSQLHGILSHTVTDLSAIHNTVLALTSQARVKDAIQCMSIAMLNAVPIVEASG--EGE 246

Query: 245 ISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQEFTN 304
                +   ++ V   + T S+ DL     C       +  +++ L  N LE +      
Sbjct: 247 DHKQLVDGKNRRV---VGTFSASDLKG---CH------LATLRSWLPLNALEFV----EK 306

Query: 305 SPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAVIIQA 364
            P ++    FT+++S                       T   E + CH  S+L  VI   
Sbjct: 307 IPRTL---LFTAATS-----------------------TPGRELVTCHVTSTLAQVIHMV 349

Query: 365 IAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHL 397
              RV+ VWV++ +  L G+V+  D++ V R  L
Sbjct: 367 TTKRVHRVWVVDQNGGLQGLVSLTDIIAVVRSAL 349

BLAST of Carg14689 vs. ExPASy Swiss-Prot
Match: Q9XI37 (SNF1-related protein kinase regulatory subunit gamma-like PV42a OS=Arabidopsis thaliana OX=3702 GN=PV42A PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 7.7e-04
Identity = 37/169 (21.89%), Postives = 83/169 (49.11%), Query Frame = 0

Query: 76  VGKLCMVDVICYLC-RDENLLSPSAALCAPISEILPQ-IPGI-VMHLEPSASLLDAIDLV 135
           +G L M+D++ ++   D NL      + + +S I+   + G+ +  L P+ S+L+ +++ 
Sbjct: 84  IGILTMLDILAHIAGEDSNLSDLDRKMSSQVSSIIGHCLEGLSLWTLNPNTSVLECMEVF 143

Query: 136 LQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRYLLSSIGHFSPIA 195
            +G    +VP+++ + SN+    ++     I     +  LTQ D++R+L     HF  + 
Sbjct: 144 SKGIHRALVPVESSIESNNTIAGVE----LIESASAYKMLTQMDLLRFLKDH--HFDDLK 203

Query: 196 AL---SLDNLGIICTNPLSVNYHSPASSAIGAISHCIRQSNLVVDADGI 239
            +   S+ +LG +  +  ++   +  S+AI  +   +  +  +V A  I
Sbjct: 204 TVLSRSISDLGAVNDSVYAITERTTVSNAINVMKGALLNAVPIVHAPDI 246

BLAST of Carg14689 vs. ExPASy TrEMBL
Match: A0A6J1FQ76 (CBS domain-containing protein CBSX5-like OS=Cucurbita moschata OX=3662 GN=LOC111445967 PE=4 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 4.5e-217
Identity = 393/402 (97.76%), Postives = 396/402 (98.51%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60
           MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD
Sbjct: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60

Query: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120
           VDGSADGGEFECCRCVGKLCMVDVICYLCRD+NLLSPSAALCAPISEILPQIPGIVMHLE
Sbjct: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDQNLLSPSAALCAPISEILPQIPGIVMHLE 120

Query: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRY 180
           PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTP NAI+GGREFCWLTQEDIIRY
Sbjct: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPKNAIHGGREFCWLTQEDIIRY 180

Query: 181 LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCI--RQSNLVVDADGI 240
           LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCI  + S  VVDADGI
Sbjct: 181 LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCIANQTSVAVVDADGI 240

Query: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300
           LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ
Sbjct: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300

Query: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360
           EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV
Sbjct: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360

Query: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 401
           IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE
Sbjct: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 402

BLAST of Carg14689 vs. ExPASy TrEMBL
Match: A0A6J1IW58 (CBS domain-containing protein CBSX5-like OS=Cucurbita maxima OX=3661 GN=LOC111480470 PE=4 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.2e-214
Identity = 389/402 (96.77%), Postives = 393/402 (97.76%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60
           MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRL KNGCGGGD
Sbjct: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLAKNGCGGGD 60

Query: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120
           VDGSADGG+FECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE
Sbjct: 61  VDGSADGGDFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120

Query: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRY 180
           PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLK PTNAI+GGREFCWLTQEDIIRY
Sbjct: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKNPTNAIHGGREFCWLTQEDIIRY 180

Query: 181 LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCI--RQSNLVVDADGI 240
           LLSSIGHFSPIAALSLDNLGIICTN LS+NYHSPASSAIGAIS CI  + S  VVDADGI
Sbjct: 181 LLSSIGHFSPIAALSLDNLGIICTNALSINYHSPASSAIGAISRCIANQTSVAVVDADGI 240

Query: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300
           LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ
Sbjct: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300

Query: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360
           EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV
Sbjct: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360

Query: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 401
           IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE
Sbjct: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 402

BLAST of Carg14689 vs. ExPASy TrEMBL
Match: A0A0A0L3G0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G637820 PE=4 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 2.1e-190
Identity = 350/402 (87.06%), Postives = 371/402 (92.29%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60
           MAVSLFSHDVSDLCLGKP LR LSLSAT+ADAL AL+FS DYFVSVWDCRL K GC G  
Sbjct: 1   MAVSLFSHDVSDLCLGKPPLRPLSLSATLADALLALQFSHDYFVSVWDCRLPKRGCTGA- 60

Query: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120
           VDG A GG+FECCRCVGKLCMVDVICYLC++ENLLSPS+AL A +SEILPQIPGIVMHLE
Sbjct: 61  VDGGAAGGDFECCRCVGKLCMVDVICYLCKEENLLSPSSALQASVSEILPQIPGIVMHLE 120

Query: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRY 180
           PSASLL+AIDLVLQGAQNLVVPIK +LGSNSRRKQLK  TN I+GG EFCWLTQEDIIRY
Sbjct: 121 PSASLLEAIDLVLQGAQNLVVPIKTRLGSNSRRKQLKNSTNGIHGGHEFCWLTQEDIIRY 180

Query: 181 LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCI--RQSNLVVDADGI 240
           LL SIG FSPIAALSLD+LGIICTN LSVNYHSPASSAIGAISH I  + S  V+D DGI
Sbjct: 181 LLGSIGLFSPIAALSLDSLGIICTNALSVNYHSPASSAIGAISHSITNQTSVAVIDGDGI 240

Query: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300
           LIGEISPFALA CDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLK++ LEGML+
Sbjct: 241 LIGEISPFALAGCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKDSKLEGMLE 300

Query: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360
           EFTNSPSSIGSPSFTSSSSDEEFSPSPSSR+YRRSSSYSARITRR+EAIVCHPRSSL+AV
Sbjct: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRRYRRSSSYSARITRRAEAIVCHPRSSLVAV 360

Query: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 401
           +IQAI HRVNYVWVIEDDCSLIG+VTFLDMLKVFREHLETSE
Sbjct: 361 MIQAITHRVNYVWVIEDDCSLIGMVTFLDMLKVFREHLETSE 401

BLAST of Carg14689 vs. ExPASy TrEMBL
Match: A0A5A7VF56 (CBS domain-containing protein CBSX5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003850 PE=4 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 9.9e-188
Identity = 348/402 (86.57%), Postives = 371/402 (92.29%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60
           MAVSLFSHDVSDLCLGKPALR LSLSAT+ADAL AL+FS DYFVSVWDC L K+GC GG 
Sbjct: 1   MAVSLFSHDVSDLCLGKPALRPLSLSATLADALLALQFSHDYFVSVWDCHLPKSGCSGG- 60

Query: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120
           VDG A GG+FECCRCVGKLCMVDV+CYLC++ENLLSPSAAL A +SEILPQIPGIVMHLE
Sbjct: 61  VDGGAAGGDFECCRCVGKLCMVDVVCYLCKEENLLSPSAALQASVSEILPQIPGIVMHLE 120

Query: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRY 180
           PSASLL+AIDLVLQGAQNLVVPIK KLGSNSRRKQLK   NAI+GGREFCWLTQEDIIRY
Sbjct: 121 PSASLLEAIDLVLQGAQNLVVPIKTKLGSNSRRKQLKNSPNAIHGGREFCWLTQEDIIRY 180

Query: 181 LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCI--RQSNLVVDADGI 240
           LLSSIG FSPIAALSLD+LGII TN LSV+YHSPASSAIGAIS  I  + S  V+D DGI
Sbjct: 181 LLSSIGFFSPIAALSLDSLGIIGTNALSVDYHSPASSAIGAISRSITNQTSVAVIDGDGI 240

Query: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300
           LIGEISPFALA CDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVK+VKARLK++ LEGML+
Sbjct: 241 LIGEISPFALAGCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKLVKARLKDSKLEGMLE 300

Query: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360
           EFTNSPSSIGS SFTSSSSDEEFSPSP SR+ RRSSSYSARITRR+EAIVCHPRSSL+AV
Sbjct: 301 EFTNSPSSIGSASFTSSSSDEEFSPSPRSRRCRRSSSYSARITRRAEAIVCHPRSSLVAV 360

Query: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 401
           +IQAIAHRVNYVWVIEDDCSLIG+VTFLDMLKVFREHLETSE
Sbjct: 361 MIQAIAHRVNYVWVIEDDCSLIGMVTFLDMLKVFREHLETSE 401

BLAST of Carg14689 vs. ExPASy TrEMBL
Match: A0A1S3BTV5 (CBS domain-containing protein CBSX5 OS=Cucumis melo OX=3656 GN=LOC103493495 PE=4 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 9.9e-188
Identity = 348/402 (86.57%), Postives = 371/402 (92.29%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60
           MAVSLFSHDVSDLCLGKPALR LSLSAT+ADAL AL+FS DYFVSVWDC L K+GC GG 
Sbjct: 1   MAVSLFSHDVSDLCLGKPALRPLSLSATLADALLALQFSHDYFVSVWDCHLPKSGCSGG- 60

Query: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120
           VDG A GG+FECCRCVGKLCMVDV+CYLC++ENLLSPSAAL A +SEILPQIPGIVMHLE
Sbjct: 61  VDGGAAGGDFECCRCVGKLCMVDVVCYLCKEENLLSPSAALQASVSEILPQIPGIVMHLE 120

Query: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRY 180
           PSASLL+AIDLVLQGAQNLVVPIK KLGSNSRRKQLK   NAI+GGREFCWLTQEDIIRY
Sbjct: 121 PSASLLEAIDLVLQGAQNLVVPIKTKLGSNSRRKQLKNSPNAIHGGREFCWLTQEDIIRY 180

Query: 181 LLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPASSAIGAISHCI--RQSNLVVDADGI 240
           LLSSIG FSPIAALSLD+LGII TN LSV+YHSPASSAIGAIS  I  + S  V+D DGI
Sbjct: 181 LLSSIGFFSPIAALSLDSLGIIGTNALSVDYHSPASSAIGAISRSITNQTSVAVIDGDGI 240

Query: 241 LIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQ 300
           LIGEISPFALA CDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVK+VKARLK++ LEGML+
Sbjct: 241 LIGEISPFALAGCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKLVKARLKDSKLEGMLE 300

Query: 301 EFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAV 360
           EFTNSPSSIGS SFTSSSSDEEFSPSP SR+ RRSSSYSARITRR+EAIVCHPRSSL+AV
Sbjct: 301 EFTNSPSSIGSASFTSSSSDEEFSPSPRSRRCRRSSSYSARITRRAEAIVCHPRSSLVAV 360

Query: 361 IIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLETSE 401
           +IQAIAHRVNYVWVIEDDCSLIG+VTFLDMLKVFREHLETSE
Sbjct: 361 MIQAIAHRVNYVWVIEDDCSLIGMVTFLDMLKVFREHLETSE 401

BLAST of Carg14689 vs. TAIR 10
Match: AT5G53750.1 (CBS domain-containing protein )

HSP 1 Score: 337.0 bits (863), Expect = 2.0e-92
Identity = 202/424 (47.64%), Postives = 282/424 (66.51%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLLSL-SATVADALTALKFSDDYFVSVWDCRLTKNGCGGG 60
           MA++L SH++SDLC+GKP LR LS+ +ATVADA+ ALK SD+ F++VW C          
Sbjct: 1   MALTLLSHELSDLCIGKPPLRCLSVATATVADAIAALKSSDEPFLTVWSC---------- 60

Query: 61  DVDGSADGGEFECCRCVGKLCMVDVICYLCR-DENLLSPSAALCAPISEILPQIPGIVMH 120
           + D   D  + + C C+GK+CM DVICYL + D N+LS S+A  A +S +LP+   +V+H
Sbjct: 61  NHDEKTD--DNDKCECLGKICMADVICYLSKFDNNVLSLSSAFDASVSVLLPKSRALVVH 120

Query: 121 LEPSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRRKQLKTPTNAIY-----------GGR 180
           ++ S SL++AIDL+++GAQNL+VPI  K  +  R++Q     N +              R
Sbjct: 121 VQSSCSLIEAIDLIIKGAQNLIVPIHTKSITKRRQQQKLLKRNVVVSLTNATSTTHKNSR 180

Query: 181 EFCWLTQEDIIRYLLSSIGHFSPIAALSLDNLGIICTNP--LSVNYHSPASSAIGAISHC 240
           EFCW+TQEDIIR+LL SI  FSP+ +LS+ +LG+I +    L+V+Y+S A+SA+ AIS  
Sbjct: 181 EFCWITQEDIIRFLLDSISVFSPLPSLSISDLGVINSTHTILAVDYYSSAASAVSAISRA 240

Query: 241 IRQSNLVV---------DADGILIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGP 300
           I  +  V          D   +LIGEISP  LA CD+   AA+ TLS+GDLM+YID  GP
Sbjct: 241 ILDNVSVAVVGKGCDQEDPCMVLIGEISPMTLACCDETAVAAVATLSAGDLMSYIDGSGP 300

Query: 301 PEDLVKVVKARLKENNLEGMLQEFTNSPSSIGSPSFTSSSSDEEFSPSPSSR---KYRRS 360
           PE LV VV+ RL++  + G++       S I S S +S SS +E SP+  +R    Y RS
Sbjct: 301 PESLVGVVRNRLEDKGMVGLI-------SLIDSLSLSSGSSSDEESPAGKTRMTSSYGRS 360

Query: 361 SSYSARITRRSEAIVCHPRSSLIAVIIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFR 398
            S +AR+ R+S AIVC+ +SSL+AV+IQAIAHRV+YVWVI++D  LIG+VTF+D+LK+FR
Sbjct: 361 VSSAARMARKSVAIVCNRKSSLMAVMIQAIAHRVSYVWVIDEDGCLIGMVTFVDILKLFR 405

BLAST of Carg14689 vs. TAIR 10
Match: AT4G27460.1 (Cystathionine beta-synthase (CBS) family protein )

HSP 1 Score: 330.5 bits (846), Expect = 1.9e-90
Identity = 194/409 (47.43%), Postives = 275/409 (67.24%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLL-SLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGG 60
           MA+SL S++VSDLCLGKP LR L S S++V+DA+ ALK S+D F+SVW+C          
Sbjct: 1   MALSLLSYNVSDLCLGKPPLRCLSSSSSSVSDAIAALKSSEDTFLSVWNC--------NH 60

Query: 61  DVDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHL 120
           D D + +      C C+GK+ M DVIC+L +D +      AL + +S +LP+   IV+H+
Sbjct: 61  DDDNNTE------CECLGKISMADVICHLSKDHD--HSLCALNSSVSVLLPKTRSIVLHV 120

Query: 121 EPSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRR--KQLKTPTNAIYGGREFCWLTQEDI 180
           +PS SL++AIDL+++GAQNL+VPI  K  +  ++    +   T     G+ FCW+TQEDI
Sbjct: 121 QPSCSLIEAIDLIIKGAQNLIVPIHTKPYTKKKQHNDNVSVTTTTHSNGQRFCWITQEDI 180

Query: 181 IRYLLSSIGHFSPIAALSLDNLGIICT--NPLSVNYHSPASSAIGAISH--CIRQSNLVV 240
           I++LL  I  FSP+ A+SL +LG+I +    ++V+YHS AS+ + A+S+   ++ S  VV
Sbjct: 181 IQFLLGFIAAFSPLPAMSLSDLGVINSTHTVVAVDYHSSASAVVSAVSNALAVQTSVAVV 240

Query: 241 DADG-----ILIGEISPFALARCDKAVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARL 300
           D +G      LIGEISP  L  CD+  AAA+ TLS+GDLMAYID   PPE LV++V+ RL
Sbjct: 241 DGEGDDPFTSLIGEISPMTLTCCDETAAAAVATLSAGDLMAYIDGANPPESLVQIVRNRL 300

Query: 301 KENNLEGMLQEFTNSPSSIGSPSFTSSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIV 360
           ++  L G++  F     S+ S S +S  S EE +P  ++  Y RS S SAR+ R+SEAIV
Sbjct: 301 EDKGLIGLMSLF----DSLSSYSTSSGYSSEEEAPVRTT-SYGRSMSSSARMARKSEAIV 360

Query: 361 CHPRSSLIAVIIQAIAHRVNYVWVIEDDCSLIGIVTFLDMLKVFREHLE 398
           C+P+SSL+AV+IQA+AHRVNY WV+E D   +G+VTF+D+LKVFR+ LE
Sbjct: 361 CNPKSSLMAVMIQAVAHRVNYAWVVEKDGCFVGMVTFVDILKVFRKFLE 388

BLAST of Carg14689 vs. TAIR 10
Match: AT1G65320.1 (Cystathionine beta-synthase (CBS) family protein )

HSP 1 Score: 109.8 bits (273), Expect = 5.2e-24
Identity = 118/428 (27.57%), Postives = 189/428 (44.16%), Query Frame = 0

Query: 1   MAVSLFSHDVSDLCLGKPALRLLSLSATVADALTALKFSDDYFVSVWDCRLTKNGCGGGD 60
           MA     H V DL +GKP +     + TV  A+ A+  S +  + VW  R T +      
Sbjct: 1   MASVFLYHVVGDLTVGKPEMVEFYETETVESAIRAIGESTECGIPVWRKRTTPS------ 60

Query: 61  VDGSADGGEFECCRCVGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIPGIVMHLE 120
           + G  +  E    R VG L  +D++ +L + E  L    A+  P+SE++     ++  ++
Sbjct: 61  LPGFVENSEMRQQRFVGILNSLDIVAFLAKTE-CLQEEKAMKIPVSEVVSPDNTLLKQVD 120

Query: 121 PSASLLDAIDLVLQGAQNLVVPIKAKLGSNSRR-------KQLKT--------------- 180
           P   L+DA++++ QG + L+VP        S+R       K LK                
Sbjct: 121 PGTRLIDALEMMKQGVRRLLVPKSVVWRGMSKRFSILYNGKWLKNSENSSSSSGLSADST 180

Query: 181 --PTNAIYGGRE-FCWLTQEDIIRYLLSSIGHFSPIAALSLDNLGIICTNPLSVNYHSPA 240
             PT ++   R+ FC L++ED+IR+L+  +G  +P+   S+  LGII  N   +    PA
Sbjct: 181 NRPTTSMTSSRDKFCCLSREDVIRFLIGVLGALAPLPLTSISTLGIINQNYNFIEASLPA 240

Query: 241 SSAI-------GAISHCIRQSNLVVDADGILIGEISPFALARCDKAVAA-AIMTLSSGDL 300
             A         AI+   +  N   +    +IGEIS   L +CD   AA A+  L +G  
Sbjct: 241 IEATRRPLCDPSAIAVLEQTEN---EQQFKIIGEISASKLWKCDYLAAAWALANLYAG-- 300

Query: 301 MAYIDCGGPPEDLVKVVKARLKENNLEGMLQEFTNSPSSIGSPSFTS----SSSDEEFSP 360
                        V  V+  +   +    LQ  T+ P    + + T+    SS    F+P
Sbjct: 301 -----------QFVMGVEDNMSSRSFSDFLQ--TSFPGGEQNGTATNAKKFSSRSIGFNP 360

Query: 361 SPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAVIIQAIAHRVNYVWVIEDDCS--LIG 390
           +  +R     S Y      RS  + C   SSL AV+ Q ++HR  +VWV E D    L+G
Sbjct: 361 TSPTRLSIGRSMYRG----RSAPLTCKTSSSLAAVMAQMLSHRATHVWVTEADSDDVLVG 399

BLAST of Carg14689 vs. TAIR 10
Match: AT1G80090.1 (Cystathionine beta-synthase (CBS) family protein )

HSP 1 Score: 57.4 bits (137), Expect = 3.1e-08
Identity = 72/323 (22.29%), Postives = 137/323 (42.41%), Query Frame = 0

Query: 76  VGKLCMVDVICYLCRDENLLSPSAALCAPISEILPQIP-GI-VMHLEPSASLLDAIDLVL 135
           +G + M+DV+ ++  D+        + AP+S I+   P G+ +  L P+ S++D ++++ 
Sbjct: 123 IGMVTMLDVVAHIAGDDGESGLDKKMAAPVSSIIGHCPEGLSLWSLNPNTSIMDCMEMLS 182

Query: 136 QGAQNLVVPIKAKLGSNSRRKQLKTPTNAIYGGREFCWLTQEDIIRYLLSSIGHFSPIAA 195
           +G   ++VP    L SN+      T    +     +  L+Q D+I +          I +
Sbjct: 183 KGIHRVLVP----LDSNTEN---ITGPELVESASAYAMLSQMDLISFFFDQSSQLHGILS 242

Query: 196 LSLDNLGIICTNPLSVNYHSPASSAIGAISHCIRQSNLVVDADGILIGEISPFALARCDK 255
            ++ +L  I    L++   +    AI  +S  +  +  +V+A G   GE     +   ++
Sbjct: 243 HTVTDLSAIHNTVLALTSQARVKDAIQCMSIAMLNAVPIVEASG--EGEDHKQLVDGKNR 302

Query: 256 AVAAAIMTLSSGDLMAYIDCGGPPEDLVKVVKARLKENNLEGMLQEFTNSPSSIGSPSFT 315
            V   + T S+ DL     C       +  +++ L  N LE +       P ++    FT
Sbjct: 303 RV---VGTFSASDLKG---CH------LATLRSWLPLNALEFV----EKIPRTL---LFT 362

Query: 316 SSSSDEEFSPSPSSRKYRRSSSYSARITRRSEAIVCHPRSSLIAVIIQAIAHRVNYVWVI 375
           +++S                       T   E + CH  S+L  VI      RV+ VWV+
Sbjct: 363 AATS-----------------------TPGRELVTCHVTSTLAQVIHMVTTKRVHRVWVV 394

Query: 376 EDDCSLIGIVTFLDMLKVFREHL 397
           + +  L G+V+  D++ V R  L
Sbjct: 423 DQNGGLQGLVSLTDIIAVVRSAL 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022940315.19.4e-21797.76CBS domain-containing protein CBSX5-like [Cucurbita moschata][more]
XP_023525010.13.6e-21697.76CBS domain-containing protein CBSX5-like [Cucurbita pepo subsp. pepo][more]
XP_022981296.12.6e-21496.77CBS domain-containing protein CBSX5-like [Cucurbita maxima][more]
XP_038898999.11.0e-19187.56CBS domain-containing protein CBSX5 [Benincasa hispida][more]
XP_004141365.14.4e-19087.06CBS domain-containing protein CBSX5 [Cucumis sativus] >KGN55142.1 hypothetical p... [more]
Match NameE-valueIdentityDescription
Q84WQ52.7e-8947.43CBS domain-containing protein CBSX5 OS=Arabidopsis thaliana OX=3702 GN=CBSX5 PE=... [more]
Q8GZA47.4e-2327.57CBS domain-containing protein CBSX6 OS=Arabidopsis thaliana OX=3702 GN=CBSX6 PE=... [more]
Q8GXI93.3e-0722.34SNF1-related protein kinase regulatory subunit gamma-like PV42b OS=Arabidopsis t... [more]
Q9XI377.7e-0421.89SNF1-related protein kinase regulatory subunit gamma-like PV42a OS=Arabidopsis t... [more]
Match NameE-valueIdentityDescription
A0A6J1FQ764.5e-21797.76CBS domain-containing protein CBSX5-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1IW581.2e-21496.77CBS domain-containing protein CBSX5-like OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A0A0L3G02.1e-19087.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G637820 PE=4 SV=1[more]
A0A5A7VF569.9e-18886.57CBS domain-containing protein CBSX5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3BTV59.9e-18886.57CBS domain-containing protein CBSX5 OS=Cucumis melo OX=3656 GN=LOC103493495 PE=4... [more]
Match NameE-valueIdentityDescription
AT5G53750.12.0e-9247.64CBS domain-containing protein [more]
AT4G27460.11.9e-9047.43Cystathionine beta-synthase (CBS) family protein [more]
AT1G65320.15.2e-2427.57Cystathionine beta-synthase (CBS) family protein [more]
AT1G80090.13.1e-0822.29Cystathionine beta-synthase (CBS) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.580.10coord: 327..394
e-value: 8.3E-6
score: 27.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 300..330
NoneNo IPR availablePANTHERPTHR13780:SF132CYSTATHIONINE BETA-SYNTHASE (CBS) FAMILY PROTEIN-RELATEDcoord: 1..398
NoneNo IPR availablePANTHERPTHR13780AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNITcoord: 1..398
NoneNo IPR availableSUPERFAMILY54631CBS-domain paircoord: 193..396
IPR000644CBS domainPFAMPF00571CBScoord: 344..391
e-value: 3.7E-4
score: 20.9
IPR000644CBS domainPROSITEPS51371CBScoord: 340..398
score: 8.904053

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14689-RACarg14689-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042149 cellular response to glucose starvation
biological_process GO:0006468 protein phosphorylation
biological_process GO:0050790 regulation of catalytic activity
cellular_component GO:0005737 cytoplasm
cellular_component GO:0031588 nucleotide-activated protein kinase complex
cellular_component GO:0005634 nucleus
molecular_function GO:0016208 AMP binding
molecular_function GO:0019901 protein kinase binding
molecular_function GO:0019887 protein kinase regulator activity