Carg14609 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg14609 vs. NCBI nr
Match: KAG7012452.1 (MACPF domain-containing protein NSL1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1313.1 bits (3397), Expect = 0.0e+00
Identity = 655/655 (100.00%), Postives = 655/655 (100.00%), Query Frame = 0

Query: 1   MSCNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60
           MSCNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA
Sbjct: 1   MSCNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120
           GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180
           QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240

Query: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300
           MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEF 360
           VPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEF
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEF 360

Query: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN 420
           QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN
Sbjct: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN 420

Query: 421 MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480
           MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK
Sbjct: 421 MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480

Query: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540
           GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA
Sbjct: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540

Query: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600
           SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA
Sbjct: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600

Query: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
           MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI
Sbjct: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 655

BLAST of Carg14609 vs. NCBI nr
Match: XP_022955162.1 (MACPF domain-containing protein NSL1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 628/655 (95.88%), Postives = 630/655 (96.18%), Query Frame = 0

Query: 1   MSCNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60
           MS NVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120
           GVVVPNVSNSIKCDKGERTRFRSDV+SFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVVSFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180
           QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAAL+KFIENYGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSED NGGFMSNSTAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDVNGGFMSNSTAAKIKDEYS 240

Query: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300
           MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEF 360
           VPITSLLNGVRGNGFLSHAVNLYLR                      KPPIEELQQFLEF
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRY---------------------KPPIEELQQFLEF 360

Query: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN 420
           QLPRQWAPVYADLPLATR RKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN
Sbjct: 361 QLPRQWAPVYADLPLATRLRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN 420

Query: 421 MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480
           MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK
Sbjct: 421 MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480

Query: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540
           GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA
Sbjct: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540

Query: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600
           SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA
Sbjct: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600

Query: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
           MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI
Sbjct: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 634

BLAST of Carg14609 vs. NCBI nr
Match: KAG6573287.1 (MACPF domain-containing protein NSL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 608/655 (92.82%), Postives = 608/655 (92.82%), Query Frame = 0

Query: 1   MSCNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60
           MS NVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120
           GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180
           QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240

Query: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300
           MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEF 360
           VPITSLLNGVRGNGFLSHAVNLYLR                      KPPIEELQQFLEF
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRY---------------------KPPIEELQQFLEF 360

Query: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN 420
           QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTK                  
Sbjct: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTK------------------ 420

Query: 421 MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480
                 VDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK
Sbjct: 421 ------VDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480

Query: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540
           GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA
Sbjct: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540

Query: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600
           SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA
Sbjct: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600

Query: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
           MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI
Sbjct: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 610

BLAST of Carg14609 vs. NCBI nr
Match: XP_022955164.1 (MACPF domain-containing protein NSL1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1184.1 bits (3062), Expect = 0.0e+00
Identity = 604/655 (92.21%), Postives = 606/655 (92.52%), Query Frame = 0

Query: 1   MSCNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60
           MS NVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120
           GVVVPNVSNSIKCDKGERTRFRSDV+SFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVVSFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180
           QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAAL+KFIENYGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSED NGGFMSNSTAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDVNGGFMSNSTAAKIKDEYS 240

Query: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300
           MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEF 360
           VPITSLLNGVRGNGFLSHAVNLYLR                      KPPIEELQQFLEF
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRY---------------------KPPIEELQQFLEF 360

Query: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN 420
           QLPRQWAPVYADLPLATR RKQASPSLKFTLMGPKLYVNTTK                  
Sbjct: 361 QLPRQWAPVYADLPLATRLRKQASPSLKFTLMGPKLYVNTTK------------------ 420

Query: 421 MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480
                 VDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK
Sbjct: 421 ------VDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480

Query: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540
           GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA
Sbjct: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540

Query: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600
           SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA
Sbjct: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600

Query: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
           MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI
Sbjct: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 610

BLAST of Carg14609 vs. NCBI nr
Match: XP_023542863.1 (MACPF domain-containing protein NSL1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1180.6 bits (3053), Expect = 0.0e+00
Identity = 602/655 (91.91%), Postives = 605/655 (92.37%), Query Frame = 0

Query: 1   MSCNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60
           MS NVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120
           GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180
           QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAAL+KFIENYGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRES+IEQAEVQKKLKQLADERFSEDANGGFMSNS AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESHIEQAEVQKKLKQLADERFSEDANGGFMSNSAAAKIKDEYS 240

Query: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300
           MPWQLRHAFAASIKPPIITHSKGDEL TIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELMTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEF 360
           VPITSLLNGVRGNGFLSHAVNLYLR                      KPPIEELQQFLEF
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRY---------------------KPPIEELQQFLEF 360

Query: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN 420
           QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTK                  
Sbjct: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTK------------------ 420

Query: 421 MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480
                 VDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK
Sbjct: 421 ------VDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480

Query: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540
           GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA
Sbjct: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540

Query: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600
           SA IRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA
Sbjct: 541 SAMIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600

Query: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
           MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTIL+EDSMI
Sbjct: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILTEDSMI 610

BLAST of Carg14609 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 5.9e-219
Identity = 394/657 (59.97%), Postives = 474/657 (72.15%), Query Frame = 0

Query: 4   NVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPAGVV 63
           N  R D  SAAEKAVSVIG G+DLC+D+R S+CK  P GS+L++ID TR +DL+ P G+V
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCWQND 123
           V NVS+SIKCDKGERTR RSD++SFNQMSE FNQ + LSGKIPS  FN+MF    CW  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVVVGV 183
           A S K+L +DG FI+LY++E+ R  +TL ++VKREVPSSWD AAL+ FIE YGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSED-ANGGFMSNSTAAKIKDEYSMP 243
            MGGKDVI ++Q+R+SN E  E+QK LK   DERF  D       ++  + K K+E  + 
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQ 244

Query: 244 WQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSFVP 303
           W L+  F  S+   ++ H+K +E+  + +RRGGVD+GQSH +WLSTVSQ+PNVISM FVP
Sbjct: 245 WGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVP 304

Query: 304 ITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEFQL 363
           ITSLL+G+ G GFLSHAVNLYLR                      KPPIEEL QFLEFQL
Sbjct: 305 ITSLLSGLPGTGFLSHAVNLYLRY---------------------KPPIEELHQFLEFQL 364

Query: 364 PRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLNMI 423
           PRQWAPVY DLPL  R  KQ+SPSL+F+LMGPKLYVNT+K                    
Sbjct: 365 PRQWAPVYGDLPLGLRRSKQSSPSLQFSLMGPKLYVNTSK-------------------- 424

Query: 424 LQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEKGY 483
               VDS  RPVTG+R FLEGKK +HLAIHLQHLS  P ++ LS D +Y PI+EP EKGY
Sbjct: 425 ----VDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEPVEKGY 484

Query: 484 FQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVASA 543
           + PVKW IFSHVCT PVQYNG+   DDTASIVTKAW EVK +GMRKVLFLRLGFS  ASA
Sbjct: 485 YVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKGMGMRKVLFLRLGFSLDASA 544

Query: 544 KIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEA----QPKIELNSAVYPDGPPLTV 603
             R+S W+  ST+SRKSG+ S  ISTR STGL    A    Q KI++NSAVYP GP   V
Sbjct: 545 VTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKIDINSAVYPRGPSPPV 604

Query: 604 RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
           +  K+L+++DTKE++RGPE  PGYWVVTGAKLCVE  +ISIK KYSLLT++SEDS++
Sbjct: 605 KP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLLTVISEDSLV 612

BLAST of Carg14609 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.8e-167
Identity = 314/655 (47.94%), Postives = 420/655 (64.12%), Query Frame = 0

Query: 7   RFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDI-DLTRTKDLVLPAGVVVP 66
           R     AAE A+  IG G+DL  DLRL  CK G   S+L+DI +     ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCWQNDAG 126
           NVS SIKCDKGER RFRSD++ F QM+E FNQ+LSL+GKIPS  FN+MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 SSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVVVGVKM 186
            +K+L FDG FI+LY++ L++S + L E VK+ VPS+WDPAAL++FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDA---NGGFMSNSTAAKIKDEYSMP 246
           GGKDVI+ +Q   S ++  ++QK+LK++AD+RF E +   N G      ++K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVD-VGQSHNQWLSTVSQSPNVISMSFV 306
            +LR A  +S+     +++  ++   +  RRGG D     HN+WL TV   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEFQ 366
           PITSLLNGV G+GFLSHA+NLYLR                      KPPIEEL QFLEFQ
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRY---------------------KPPIEELHQFLEFQ 363

Query: 367 LPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLNM 426
           LPRQWAPV+++LPL  + ++Q+  SL+F+  GPKLYVNTT                    
Sbjct: 364 LPRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKLYVNTT-------------------- 423

Query: 427 ILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEKG 486
                VD   RP+TG+RL+LEG++S+ LAIHLQHLS+LPK  QL DDL+     E  ++ 
Sbjct: 424 ----PVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSIRQESHDRR 483

Query: 487 YFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVAS 546
           Y++ V W  +SHVCT PV+       DD  S+VT A   V+  G + VLFLRL FS V  
Sbjct: 484 YYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHGFKNVLFLRLCFSRVVG 543

Query: 547 AK-IRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 606
           A  ++ SEW+     + KSGLIST IS  F+   + P     + +NSA+YP GPP+  +A
Sbjct: 544 ATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPPVPTQA 600

Query: 607 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
            K+L  +DT EM RGP+ SPGYWVV+GA+L VE  +IS+KVKYSL T +  D +I
Sbjct: 604 PKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSLFTPILGDEVI 600

BLAST of Carg14609 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 2.9e-117
Identity = 251/666 (37.69%), Postives = 370/666 (55.56%), Query Frame = 0

Query: 9   DPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGS---KLIDIDLTRTKDLVLPAGVVVP 68
           D     E AV  +G GFDL  D RL  CK G   +   +L+ +D T+ ++L +P   V  
Sbjct: 4   DGGDVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQ 63

Query: 69  NVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLK-GCWQNDA 128
           NVS  I CDKGERTRFRSD++ FN+MSE FNQ+ S++GKIPS  FN+ FG + G W  DA
Sbjct: 64  NVSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDA 123

Query: 129 GSSKSLGFDGCFITLYNIELER-SHITLSEQVKREVPSSWDPAALSKFIENYGTHVVVGV 188
            + KSLG D   +TL+N+ +   + + L+++V+  VPSSWDP  L++FIE YGTHV+ GV
Sbjct: 124 ANVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGV 183

Query: 189 KMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDA-------NGGFMSNSTAAKIK 248
            +GG+DV+ +RQ + S+++   ++  L  L D+ F+          N  +  + +  K  
Sbjct: 184 SVGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFP 243

Query: 249 DEYSMPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVI 308
           + +++          +     I    G  +T I  +RGG    +SH++WL TV   P+ I
Sbjct: 244 EAFNV---FDDKQTVAFNNFSINSQNG--ITVICAKRGGDGRAKSHSEWLITVPDKPDAI 303

Query: 309 SMSFVPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQ 368
           + +F+PITSLL  V G+G LSHA++LYLR                      KPP+ +LQ 
Sbjct: 304 NFNFIPITSLLKDVPGSGLLSHAMSLYLRY---------------------KPPLMDLQY 363

Query: 369 FLEFQLPRQWAPVYADLPL-ATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMT 428
           FL+F  PR WAPV+ DLP  A  +   A P+L    MGPKLYVNTT              
Sbjct: 364 FLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHINFMGPKLYVNTT-------------- 423

Query: 429 MLRLNMILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTI--QLSDDLSYGP 488
                      V S   PVTG+R FLEGKK + LAIHLQHL     T+  +++D+  +  
Sbjct: 424 ----------PVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHIWRG 483

Query: 489 IDE-PEEKGYFQPVKWSIFSHVCTAPVQYNGSLI--------DDDTASIVTKAWFEVKVI 548
            D+  +   YF+P+    FSHVCT PV+Y+ + I         +D A IVT A  EVK  
Sbjct: 484 SDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGAQLEVKKH 543

Query: 549 GMRKVLFLRLGFSTVASAKIRRSEW-EGPSTSSRKSGLISTFISTRFSTGLQQPEAQP-- 608
           G + VL LRL ++ V+   + ++ W  GP  +S+KSG+ S+      S  +     Q   
Sbjct: 544 GSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHHNMIQKDK 603

Query: 609 -KIELNSAVYPDGPPLTVRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIK 647
            ++ L+S V+P GPP+     K++  +D  ++ RGP++SPG+W+VTG +L ++  ++ + 
Sbjct: 604 NEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLDKGKLCLH 618

BLAST of Carg14609 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.1e-105
Identity = 236/641 (36.82%), Postives = 345/641 (53.82%), Query Frame = 0

Query: 17  AVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPAGVVVPNVSNSIKCDKG 76
           A+  +G GFD+ +D+RL  CK  P GS+L+ I+  + +DL L  G ++PNV   I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCWQNDAGSSKSLGFDGCF 136
                R  V SF++M+E FN +  + G IP   FN+MF   G WQ DA S+KSL   G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVVVGVKMGGKDVIFMRQL 196
           I LY+++L +  + L  +++R VPSSWDPA+L+ FIENYGTH+V  V +GG+DV+++RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYSMPWQLRHAFAASIKPP 256
           + S +  +E++  +  +   RF E                           A + SI  P
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHE---------------------------AESQSITGP 263

Query: 257 IITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSFVPITSLLNGVRGNGFL 316
           +    K  ++T I+ RRGG D+ QSH +W  TV  +P++I+M+F PI SLL GV G   L
Sbjct: 264 L--KYKDKDITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 323

Query: 317 SHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEFQLPRQWAPVYADLPLA 376
           + A+ LYL                       KPPIE+LQ FL++Q+ R WAP  ++L   
Sbjct: 324 TRAIELYLEY---------------------KPPIEDLQYFLDYQIARAWAPEQSNL--- 383

Query: 377 TRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLNMILQLQVDSNSRPVTG 436
            + ++    SL+F+LMGPKL+++                          QV    +PVTG
Sbjct: 384 -QRKEPVCSSLQFSLMGPKLFISAD------------------------QVTVGRKPVTG 443

Query: 437 VRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEKG-------YFQPVKWS 496
           +RL LEG K + L+IHLQHL +LPK +Q   D S+ PI  P+ +G       +F+P+KW 
Sbjct: 444 LRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKWQGPEEQDSRWFEPIKWK 503

Query: 497 IFSHVCTAPVQYNGSLIDD-DTASIVTKAWFEVKVIGMRKVLFLRLGFSTVASAKIRRSE 556
            FSHV T+P+++  + I D     IVT A   V   G + VL L+L FS V    IRRS 
Sbjct: 504 NFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCTIRRSV 559

Query: 557 WEGPSTSSR---KSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRAMKMLN 616
           W+    +S    + G  ST  ST   +G                         ++ K+  
Sbjct: 564 WDHTPVASSGRLEPGGPSTSSSTEEVSG-------------------------QSGKLAK 559

Query: 617 VIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLL 647
           ++D+ EM++GP++ PG+W+VTGAKL VE  +I ++VKYSLL
Sbjct: 624 IVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of Carg14609 vs. ExPASy TrEMBL
Match: A0A6J1GSU0 (MACPF domain-containing protein NSL1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457211 PE=4 SV=1)

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 628/655 (95.88%), Postives = 630/655 (96.18%), Query Frame = 0

Query: 1   MSCNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60
           MS NVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120
           GVVVPNVSNSIKCDKGERTRFRSDV+SFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVVSFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180
           QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAAL+KFIENYGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSED NGGFMSNSTAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDVNGGFMSNSTAAKIKDEYS 240

Query: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300
           MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEF 360
           VPITSLLNGVRGNGFLSHAVNLYLR                      KPPIEELQQFLEF
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRY---------------------KPPIEELQQFLEF 360

Query: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN 420
           QLPRQWAPVYADLPLATR RKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN
Sbjct: 361 QLPRQWAPVYADLPLATRLRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN 420

Query: 421 MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480
           MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK
Sbjct: 421 MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480

Query: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540
           GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA
Sbjct: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540

Query: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600
           SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA
Sbjct: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600

Query: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
           MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI
Sbjct: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 634

BLAST of Carg14609 vs. ExPASy TrEMBL
Match: A0A6J1GVB1 (MACPF domain-containing protein NSL1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457211 PE=4 SV=1)

HSP 1 Score: 1184.1 bits (3062), Expect = 0.0e+00
Identity = 604/655 (92.21%), Postives = 606/655 (92.52%), Query Frame = 0

Query: 1   MSCNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60
           MS NVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120
           GVVVPNVSNSIKCDKGERTRFRSDV+SFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVVSFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180
           QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAAL+KFIENYGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSED NGGFMSNSTAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDVNGGFMSNSTAAKIKDEYS 240

Query: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300
           MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEF 360
           VPITSLLNGVRGNGFLSHAVNLYLR                      KPPIEELQQFLEF
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRY---------------------KPPIEELQQFLEF 360

Query: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN 420
           QLPRQWAPVYADLPLATR RKQASPSLKFTLMGPKLYVNTTK                  
Sbjct: 361 QLPRQWAPVYADLPLATRLRKQASPSLKFTLMGPKLYVNTTK------------------ 420

Query: 421 MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480
                 VDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK
Sbjct: 421 ------VDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480

Query: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540
           GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA
Sbjct: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540

Query: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600
           SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA
Sbjct: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600

Query: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
           MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI
Sbjct: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 610

BLAST of Carg14609 vs. ExPASy TrEMBL
Match: A0A6J1JYX1 (MACPF domain-containing protein NSL1 OS=Cucurbita maxima OX=3661 GN=LOC111490102 PE=4 SV=1)

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 599/655 (91.45%), Postives = 603/655 (92.06%), Query Frame = 0

Query: 1   MSCNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60
           MS NVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120
           GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180
           QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAAL+KFIENYGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNIEQ EVQKKLKQLADERFSEDANGGFMSNS AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQTEVQKKLKQLADERFSEDANGGFMSNSAAAKIKDEYS 240

Query: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300
           MPWQLRHAFAASIKPPIITHSKGDEL TIYVRRGGVDVGQSHNQWLSTVSQSPNVI MSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELMTIYVRRGGVDVGQSHNQWLSTVSQSPNVILMSF 300

Query: 301 VPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEF 360
           VPITSL+NGVRGNGFLSHAVNLYLR                      KPPIEELQQFLEF
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRY---------------------KPPIEELQQFLEF 360

Query: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN 420
           QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTK                  
Sbjct: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTK------------------ 420

Query: 421 MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480
                 VDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKT+QLSDDLSYGPIDEPEEK
Sbjct: 421 ------VDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSYGPIDEPEEK 480

Query: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540
           GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA
Sbjct: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540

Query: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600
           SA IRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA
Sbjct: 541 SAMIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600

Query: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
           MKMLN+IDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI
Sbjct: 601 MKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 610

BLAST of Carg14609 vs. ExPASy TrEMBL
Match: A0A5D3DSF4 (MACPF domain-containing protein NSL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G001020 PE=4 SV=1)

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 554/655 (84.58%), Postives = 586/655 (89.47%), Query Frame = 0

Query: 1   MSCNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60
           MS N+NRF+PQSAAEKAVSV+GFG+DLCNDLRLSSCKPGPSGSKLIDID TR++DLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120
           GVVVPNV  SI CDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SHITLSEQVKREVPSSWDPAAL++FIE YGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQ +VQKKLK+LAD++FSEDANGGF SNS AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240

Query: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDELT IY+RRGGVDVGQSHNQWLST+SQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEF 360
           VPITSL+NGVRGNGFLSHAVNLYLR                      KPPIEELQQFLEF
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRY---------------------KPPIEELQQFLEF 360

Query: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN 420
           QLPRQWAPVYADLPLA RHRKQASPSLKFTLMGPKLYVNTTK                  
Sbjct: 361 QLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLYVNTTK------------------ 420

Query: 421 MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480
                 VDSN+RPVTGVRLFLEGKKSDHLAIHLQHLSTLPKT+QLSDDLSY PIDEPEEK
Sbjct: 421 ------VDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSYRPIDEPEEK 480

Query: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540
           GYF+PVKWSIFSHVCTAPV+Y+ SLIDDD+ASIVTKAWFEVKVIGMRKVLFLRLGFS VA
Sbjct: 481 GYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIGMRKVLFLRLGFSMVA 540

Query: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600
           SA IRRS+WEGPST+SRKSGLISTFISTRFS GL QPE QPKIELNSAVYPDGPPLT RA
Sbjct: 541 SAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYPDGPPLTSRA 600

Query: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
           MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSLLTILSEDSMI
Sbjct: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLLTILSEDSMI 610

BLAST of Carg14609 vs. ExPASy TrEMBL
Match: A0A0A0LV66 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G075560 PE=4 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 552/655 (84.27%), Postives = 582/655 (88.85%), Query Frame = 0

Query: 1   MSCNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60
           MS N+NRF+PQSAAEKAVSVIGFG+DLCNDLRLSSCKPGPSGSKLIDID TR++DLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120
           GVVVPNV  SI CDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SHITLSEQVKREVPSSWDPAAL++FIE YGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQ +VQKKLK+LAD++FSEDANGG  SN  AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240

Query: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDELT IY+RRGGVDVGQSHNQWLST+SQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEF 360
           VPITSL+NGVRGNGFLSHAVNLYLR                      KPPIEELQQFLEF
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRY---------------------KPPIEELQQFLEF 360

Query: 361 QLPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLN 420
           QLPRQWAPVYADLPLA RHRKQASPSLKFTLMGPKLYVNTTK                  
Sbjct: 361 QLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLYVNTTK------------------ 420

Query: 421 MILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEK 480
                 VDSN+ PVTGVRLFLEGKKSDHLAIHLQHLSTLPKT+QLSDDLSY PIDEPEEK
Sbjct: 421 ------VDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSYRPIDEPEEK 480

Query: 481 GYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVA 540
           GYF+PVKWSIFSHVCTAPV+YNGSLIDDD+ASIVTKAWFEVK IGMRKVLFLRLGFS VA
Sbjct: 481 GYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMRKVLFLRLGFSMVA 540

Query: 541 SAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 600
           SA IRRS+WEGPST+SRKSGLISTFISTRFS GL QPE QPKIELNSAVYPDGPPLT RA
Sbjct: 541 SAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYPDGPPLTSRA 600

Query: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
           MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSLLTILSEDSMI
Sbjct: 601 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLLTILSEDSMI 610

BLAST of Carg14609 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 761.9 bits (1966), Expect = 4.2e-220
Identity = 394/657 (59.97%), Postives = 474/657 (72.15%), Query Frame = 0

Query: 4   NVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPAGVV 63
           N  R D  SAAEKAVSVIG G+DLC+D+R S+CK  P GS+L++ID TR +DL+ P G+V
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCWQND 123
           V NVS+SIKCDKGERTR RSD++SFNQMSE FNQ + LSGKIPS  FN+MF    CW  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVVVGV 183
           A S K+L +DG FI+LY++E+ R  +TL ++VKREVPSSWD AAL+ FIE YGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSED-ANGGFMSNSTAAKIKDEYSMP 243
            MGGKDVI ++Q+R+SN E  E+QK LK   DERF  D       ++  + K K+E  + 
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQ 244

Query: 244 WQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSFVP 303
           W L+  F  S+   ++ H+K +E+  + +RRGGVD+GQSH +WLSTVSQ+PNVISM FVP
Sbjct: 245 WGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVP 304

Query: 304 ITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEFQL 363
           ITSLL+G+ G GFLSHAVNLYLR                      KPPIEEL QFLEFQL
Sbjct: 305 ITSLLSGLPGTGFLSHAVNLYLRY---------------------KPPIEELHQFLEFQL 364

Query: 364 PRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLNMI 423
           PRQWAPVY DLPL  R  KQ+SPSL+F+LMGPKLYVNT+K                    
Sbjct: 365 PRQWAPVYGDLPLGLRRSKQSSPSLQFSLMGPKLYVNTSK-------------------- 424

Query: 424 LQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEKGY 483
               VDS  RPVTG+R FLEGKK +HLAIHLQHLS  P ++ LS D +Y PI+EP EKGY
Sbjct: 425 ----VDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEPVEKGY 484

Query: 484 FQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVASA 543
           + PVKW IFSHVCT PVQYNG+   DDTASIVTKAW EVK +GMRKVLFLRLGFS  ASA
Sbjct: 485 YVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKGMGMRKVLFLRLGFSLDASA 544

Query: 544 KIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEA----QPKIELNSAVYPDGPPLTV 603
             R+S W+  ST+SRKSG+ S  ISTR STGL    A    Q KI++NSAVYP GP   V
Sbjct: 545 VTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKIDINSAVYPRGPSPPV 604

Query: 604 RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
           +  K+L+++DTKE++RGPE  PGYWVVTGAKLCVE  +ISIK KYSLLT++SEDS++
Sbjct: 605 KP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLLTVISEDSLV 612

BLAST of Carg14609 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 590.9 bits (1522), Expect = 1.3e-168
Identity = 314/655 (47.94%), Postives = 420/655 (64.12%), Query Frame = 0

Query: 7   RFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDI-DLTRTKDLVLPAGVVVP 66
           R     AAE A+  IG G+DL  DLRL  CK G   S+L+DI +     ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCWQNDAG 126
           NVS SIKCDKGER RFRSD++ F QM+E FNQ+LSL+GKIPS  FN+MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 SSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVVVGVKM 186
            +K+L FDG FI+LY++ L++S + L E VK+ VPS+WDPAAL++FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDA---NGGFMSNSTAAKIKDEYSMP 246
           GGKDVI+ +Q   S ++  ++QK+LK++AD+RF E +   N G      ++K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVD-VGQSHNQWLSTVSQSPNVISMSFV 306
            +LR A  +S+     +++  ++   +  RRGG D     HN+WL TV   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEFQ 366
           PITSLLNGV G+GFLSHA+NLYLR                      KPPIEEL QFLEFQ
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRY---------------------KPPIEELHQFLEFQ 363

Query: 367 LPRQWAPVYADLPLATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLNM 426
           LPRQWAPV+++LPL  + ++Q+  SL+F+  GPKLYVNTT                    
Sbjct: 364 LPRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKLYVNTT-------------------- 423

Query: 427 ILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEKG 486
                VD   RP+TG+RL+LEG++S+ LAIHLQHLS+LPK  QL DDL+     E  ++ 
Sbjct: 424 ----PVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSIRQESHDRR 483

Query: 487 YFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIGMRKVLFLRLGFSTVAS 546
           Y++ V W  +SHVCT PV+       DD  S+VT A   V+  G + VLFLRL FS V  
Sbjct: 484 YYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHGFKNVLFLRLCFSRVVG 543

Query: 547 AK-IRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRA 606
           A  ++ SEW+     + KSGLIST IS  F+   + P     + +NSA+YP GPP+  +A
Sbjct: 544 ATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPPVPTQA 600

Query: 607 MKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLLTILSEDSMI 656
            K+L  +DT EM RGP+ SPGYWVV+GA+L VE  +IS+KVKYSL T +  D +I
Sbjct: 604 PKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSLFTPILGDEVI 600

BLAST of Carg14609 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 424.1 bits (1089), Expect = 2.1e-118
Identity = 251/666 (37.69%), Postives = 370/666 (55.56%), Query Frame = 0

Query: 9   DPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGS---KLIDIDLTRTKDLVLPAGVVVP 68
           D     E AV  +G GFDL  D RL  CK G   +   +L+ +D T+ ++L +P   V  
Sbjct: 4   DGGDVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQ 63

Query: 69  NVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLK-GCWQNDA 128
           NVS  I CDKGERTRFRSD++ FN+MSE FNQ+ S++GKIPS  FN+ FG + G W  DA
Sbjct: 64  NVSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDA 123

Query: 129 GSSKSLGFDGCFITLYNIELER-SHITLSEQVKREVPSSWDPAALSKFIENYGTHVVVGV 188
            + KSLG D   +TL+N+ +   + + L+++V+  VPSSWDP  L++FIE YGTHV+ GV
Sbjct: 124 ANVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGV 183

Query: 189 KMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDA-------NGGFMSNSTAAKIK 248
            +GG+DV+ +RQ + S+++   ++  L  L D+ F+          N  +  + +  K  
Sbjct: 184 SVGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFP 243

Query: 249 DEYSMPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVI 308
           + +++          +     I    G  +T I  +RGG    +SH++WL TV   P+ I
Sbjct: 244 EAFNV---FDDKQTVAFNNFSINSQNG--ITVICAKRGGDGRAKSHSEWLITVPDKPDAI 303

Query: 309 SMSFVPITSLLNGVRGNGFLSHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQ 368
           + +F+PITSLL  V G+G LSHA++LYLR                      KPP+ +LQ 
Sbjct: 304 NFNFIPITSLLKDVPGSGLLSHAMSLYLRY---------------------KPPLMDLQY 363

Query: 369 FLEFQLPRQWAPVYADLPL-ATRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMT 428
           FL+F  PR WAPV+ DLP  A  +   A P+L    MGPKLYVNTT              
Sbjct: 364 FLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHINFMGPKLYVNTT-------------- 423

Query: 429 MLRLNMILQLQVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTI--QLSDDLSYGP 488
                      V S   PVTG+R FLEGKK + LAIHLQHL     T+  +++D+  +  
Sbjct: 424 ----------PVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHIWRG 483

Query: 489 IDE-PEEKGYFQPVKWSIFSHVCTAPVQYNGSLI--------DDDTASIVTKAWFEVKVI 548
            D+  +   YF+P+    FSHVCT PV+Y+ + I         +D A IVT A  EVK  
Sbjct: 484 SDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGAQLEVKKH 543

Query: 549 GMRKVLFLRLGFSTVASAKIRRSEW-EGPSTSSRKSGLISTFISTRFSTGLQQPEAQP-- 608
           G + VL LRL ++ V+   + ++ W  GP  +S+KSG+ S+      S  +     Q   
Sbjct: 544 GSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHHNMIQKDK 603

Query: 609 -KIELNSAVYPDGPPLTVRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIK 647
            ++ L+S V+P GPP+     K++  +D  ++ RGP++SPG+W+VTG +L ++  ++ + 
Sbjct: 604 NEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLDKGKLCLH 618

BLAST of Carg14609 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 385.6 bits (989), Expect = 8.1e-107
Identity = 236/641 (36.82%), Postives = 345/641 (53.82%), Query Frame = 0

Query: 17  AVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPAGVVVPNVSNSIKCDKG 76
           A+  +G GFD+ +D+RL  CK  P GS+L+ I+  + +DL L  G ++PNV   I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCWQNDAGSSKSLGFDGCF 136
                R  V SF++M+E FN +  + G IP   FN+MF   G WQ DA S+KSL   G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVVVGVKMGGKDVIFMRQL 196
           I LY+++L +  + L  +++R VPSSWDPA+L+ FIENYGTH+V  V +GG+DV+++RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYSMPWQLRHAFAASIKPP 256
           + S +  +E++  +  +   RF E                           A + SI  P
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHE---------------------------AESQSITGP 263

Query: 257 IITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSFVPITSLLNGVRGNGFL 316
           +    K  ++T I+ RRGG D+ QSH +W  TV  +P++I+M+F PI SLL GV G   L
Sbjct: 264 L--KYKDKDITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 323

Query: 317 SHAVNLYLRCKYPSLAVNFCLSMANGLFFNDKPPIEELQQFLEFQLPRQWAPVYADLPLA 376
           + A+ LYL                       KPPIE+LQ FL++Q+ R WAP  ++L   
Sbjct: 324 TRAIELYLEY---------------------KPPIEDLQYFLDYQIARAWAPEQSNL--- 383

Query: 377 TRHRKQASPSLKFTLMGPKLYVNTTKVIFRLEDGFVKMTMLRLNMILQLQVDSNSRPVTG 436
            + ++    SL+F+LMGPKL+++                          QV    +PVTG
Sbjct: 384 -QRKEPVCSSLQFSLMGPKLFISAD------------------------QVTVGRKPVTG 443

Query: 437 VRLFLEGKKSDHLAIHLQHLSTLPKTIQLSDDLSYGPIDEPEEKG-------YFQPVKWS 496
           +RL LEG K + L+IHLQHL +LPK +Q   D S+ PI  P+ +G       +F+P+KW 
Sbjct: 444 LRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKWQGPEEQDSRWFEPIKWK 503

Query: 497 IFSHVCTAPVQYNGSLIDD-DTASIVTKAWFEVKVIGMRKVLFLRLGFSTVASAKIRRSE 556
            FSHV T+P+++  + I D     IVT A   V   G + VL L+L FS V    IRRS 
Sbjct: 504 NFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCTIRRSV 559

Query: 557 WEGPSTSSR---KSGLISTFISTRFSTGLQQPEAQPKIELNSAVYPDGPPLTVRAMKMLN 616
           W+    +S    + G  ST  ST   +G                         ++ K+  
Sbjct: 564 WDHTPVASSGRLEPGGPSTSSSTEEVSG-------------------------QSGKLAK 559

Query: 617 VIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLL 647
           ++D+ EM++GP++ PG+W+VTGAKL VE  +I ++VKYSLL
Sbjct: 624 IVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of Carg14609 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 325.5 bits (833), Expect = 1.0e-88
Identity = 166/326 (50.92%), Postives = 226/326 (69.33%), Query Frame = 0

Query: 7   RFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDI-DLTRTKDLVLPAGVVVP 66
           R     AAE A+  IG G+DL  DLRL  CK G   S+L+DI +     ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCWQNDAG 126
           NVS SIKCDKGER RFRSD++ F QM+E FNQ+LSL+GKIPS  FN+MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 SSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVVVGVKM 186
            +K+L FDG FI+LY++ L++S + L E VK+ VPS+WDPAAL++FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDA---NGGFMSNSTAAKIKDEYSMP 246
           GGKDVI+ +Q   S ++  ++QK+LK++AD+RF E +   N G      ++K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVD-VGQSHNQWLSTVSQSPNVISMSFV 306
            +LR A  +S+     +++  ++   +  RRGG D     HN+WL TV   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLLNGVRGNGFLSHAVNLYLRCK 328
           PITSLLNGV G+GFLSHA+NLYLRCK
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRCK 322