Carg14568 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg14568
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	WRKY protein
Location	Carg_Chr18: 2515409 .. 2516948 (+)
RNA-Seq Expression	Carg14568
Synteny	Carg14568

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGCCACCTTCAACAACAACCGCCACCAATTCTATTCCGGTGATCCCTTAGATGATAGCGACGAGAAAGGCCCTGATTCTCCGCCTTCTACTTCCGCCACTGCCGCCGCCGCCAAGAAGGGCAGGTCAGCTCCTAATTTGATTAACCCAATTGCTTAATTCCATGGGGTTTCATTTTTTTTCTTTATTTATGTATATGTGATTTGTAGAAGAGGGATGAAGAAGAAAGTGGTGTCGGTGAGAATTAATGGTGATACCCCAAGGAATTCTTCTGGTTCTACTACTCCTCCTCCTGATTCTTGGGCTTGGAGAAAGTATGGTCAAAAACCCATCAAAGGCTCCCCTTATCCAAGGTGAGCTCAATTTCAAATTTTATGATATGGGTTTTGTTTCAATTTGGTCCTTCTTATCTGGGTTTCGATATTTCGTTTCAATTTGATCTTTATATACAACGGCCCAAATCTACGCACGAGCAGATATTGTCAGTTTTGTGCTTTCTGTTTTGAGATTTTTCTCAAAGTTTTTAAAATGTGATTAGTAGGGAGAGGTTTCCACGCCCTTACGAGGAATGTTTCATTCTCTTTCTAACCGATGTTGAATCTCACAATTCATTCTCTTCAATGTCCAGCATCCTTGCTAGTACTCGTCCCTTTCTTCAATCGATGTGGGACCCCCACCATCTCCCCTTTCAGATTCTAGCATCGTGGCACATCACCTTATGTCCACCCCCTCTGAGACTCATCCTCCTCGCTCGCACATCAAATTGTGTCTAGCTCTAATACCATTTGTAACATCTCAAGCCCACCGCTACCGGTATTTGTCTTTTTGGGCTTCCCTTAAAATGCGTCCGCTAGATTTTCACACCCTAATAAACGATGTTTCATTTTCCTACCTAATTGACGTGGAATCTCACATTCTTATCTGGGTTTAGTTCTTTCGTTTCGATTTGGTCTCTATAATTTTAAAAGTTTCGATTTTTTTTAGGGCGTATTATAGGTGTAGTAGTTCAAAGGGATGTCCGGCGAGGAAACAAGTAGAGAGGAATCGGCTTGATCCAACCATGTTGGTGATTTCATATTCGTGTGAACATAATCACTCCGGTCCAGTTTCTAGAAACATCAATAATCACCACCAAGCCGCCGCCGTGGAGAAACCCGGTTCGCCGGAGAGTGTACCGGTTCACCCAGAACCGGAACCGGAAGTGGAAGAGAAGTTCGTGGATATGGGAGAGGAGTCGTTGATAACGGGGGACGAGTTCAGTTGGTTCGGGGAAATGGAAACGACGTCGTCCACTGTTCTCGAGAACTCCATTTTTTCTGGATGCTCGAGTGGATTGGGAGATTCGATTTCAAGTGACGTGGCAATGCTTTTTCCGATGGGAGATAATGACGTGGACGACTCACTGTTTGCGGATCTCGGGGAGTTGCCGGAGTGTTCGTTGGTGTTCCGGCACGGCGGAGCCCGGGGATTGGCGGCGGATGAACAACCGGCTGCCGGGCAGCGGCGGATCACTCCCTGGTGCGGAACCACCACATGA

mRNA sequence

ATGGATGCCACCTTCAACAACAACCGCCACCAATTCTATTCCGGTGATCCCTTAGATGATAGCGACGAGAAAGGCCCTGATTCTCCGCCTTCTACTTCCGCCACTGCCGCCGCCGCCAAGAAGGGCAGAAGAGGGATGAAGAAGAAAGTGGTGTCGGTGAGAATTAATGGTGATACCCCAAGGAATTCTTCTGGTTCTACTACTCCTCCTCCTGATTCTTGGGCTTGGAGAAAGTATGAGAGGAATCGGCTTGATCCAACCATGTTGGTGATTTCATATTCGTGTGAACATAATCACTCCGGTCCAGTTTCTAGAAACATCAATAATCACCACCAAGCCGCCGCCGTGGAGAAACCCGGTTCGCCGGAGAGTGTACCGGTTCACCCAGAACCGGAACCGGAAGTGGAAGAGAAGTTCGTGGATATGGGAGAGGAGTCGTTGATAACGGGGGACGAGTTCAGTTGGTTCGGGGAAATGGAAACGACGTCGTCCACTGTTCTCGAGAACTCCATTTTTTCTGGATGCTCGAGTGGATTGGGAGATTCGATTTCAAGTGACGTGGCAATGCTTTTTCCGATGGGAGATAATGACGTGGACGACTCACTGTTTGCGGATCTCGGGGAGTTGCCGGAGTGTTCGTTGGTGTTCCGGCACGGCGGAGCCCGGGGATTGGCGGCGGATGAACAACCGGCTGCCGGGCAGCGGCGGATCACTCCCTGGTGCGGAACCACCACATGA

Coding sequence (CDS)

ATGGATGCCACCTTCAACAACAACCGCCACCAATTCTATTCCGGTGATCCCTTAGATGATAGCGACGAGAAAGGCCCTGATTCTCCGCCTTCTACTTCCGCCACTGCCGCCGCCGCCAAGAAGGGCAGAAGAGGGATGAAGAAGAAAGTGGTGTCGGTGAGAATTAATGGTGATACCCCAAGGAATTCTTCTGGTTCTACTACTCCTCCTCCTGATTCTTGGGCTTGGAGAAAGTATGAGAGGAATCGGCTTGATCCAACCATGTTGGTGATTTCATATTCGTGTGAACATAATCACTCCGGTCCAGTTTCTAGAAACATCAATAATCACCACCAAGCCGCCGCCGTGGAGAAACCCGGTTCGCCGGAGAGTGTACCGGTTCACCCAGAACCGGAACCGGAAGTGGAAGAGAAGTTCGTGGATATGGGAGAGGAGTCGTTGATAACGGGGGACGAGTTCAGTTGGTTCGGGGAAATGGAAACGACGTCGTCCACTGTTCTCGAGAACTCCATTTTTTCTGGATGCTCGAGTGGATTGGGAGATTCGATTTCAAGTGACGTGGCAATGCTTTTTCCGATGGGAGATAATGACGTGGACGACTCACTGTTTGCGGATCTCGGGGAGTTGCCGGAGTGTTCGTTGGTGTTCCGGCACGGCGGAGCCCGGGGATTGGCGGCGGATGAACAACCGGCTGCCGGGCAGCGGCGGATCACTCCCTGGTGCGGAACCACCACATGA

Protein sequence

MDATFNNNRHQFYSGDPLDDSDEKGPDSPPSTSATAAAAKKGRRGMKKKVVSVRINGDTPRNSSGSTTPPPDSWAWRKYERNRLDPTMLVISYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEESLITGDEFSWFGEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFADLGELPECSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT

Homology

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BLAST of Carg14568 vs. NCBI nr
Match: KAG7012492.1 (putative WRKY transcription factor 65, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 494.6 bits (1272), Expect = 5.0e-136
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   MDATFNNNRHQFYSGDPLDDSDEKGPDSPPSTSATAAAAKKGRRGMKKKVVSVRINGDTP 60
           MDATFNNNRHQFYSGDPLDDSDEKGPDSPPSTSATAAAAKKGRRGMKKKVVSVRINGDTP
Sbjct: 1   MDATFNNNRHQFYSGDPLDDSDEKGPDSPPSTSATAAAAKKGRRGMKKKVVSVRINGDTP 60

Query: 61  RNSSGSTTPPPDSWAWRKYERNRLDPTMLVISYSCEHNHSGPVSRNINNHHQAAAVEKPG 120
           RNSSGSTTPPPDSWAWRKYERNRLDPTMLVISYSCEHNHSGPVSRNINNHHQAAAVEKPG
Sbjct: 61  RNSSGSTTPPPDSWAWRKYERNRLDPTMLVISYSCEHNHSGPVSRNINNHHQAAAVEKPG 120

Query: 121 SPESVPVHPEPEPEVEEKFVDMGEESLITGDEFSWFGEMETTSSTVLENSIFSGCSSGLG 180
           SPESVPVHPEPEPEVEEKFVDMGEESLITGDEFSWFGEMETTSSTVLENSIFSGCSSGLG
Sbjct: 121 SPESVPVHPEPEPEVEEKFVDMGEESLITGDEFSWFGEMETTSSTVLENSIFSGCSSGLG 180

Query: 181 DSISSDVAMLFPMGDNDVDDSLFADLGELPECSLVFRHGGARGLAADEQPAAGQRRITPW 240
           DSISSDVAMLFPMGDNDVDDSLFADLGELPECSLVFRHGGARGLAADEQPAAGQRRITPW
Sbjct: 181 DSISSDVAMLFPMGDNDVDDSLFADLGELPECSLVFRHGGARGLAADEQPAAGQRRITPW 240

Query: 241 CGTTT 246
           CGTTT
Sbjct: 241 CGTTT 245

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BLAST of Carg14568 vs. NCBI nr
Match: XP_022954900.1 (probable WRKY transcription factor 65 [Cucurbita moschata] >KAG6573324.1 putative WRKY transcription factor 65, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 477.2 bits (1227), Expect = 8.2e-131
Identity = 244/274 (89.05%), Postives = 244/274 (89.05%), Query Frame = 0


Query: 1   MDATFNNNRHQFYSGDPLDDSDEKGPDSPPSTSATAAAAKKGRRGMKKKVVSVRINGDTP 60
           MDATFNNNRHQFYSGDPLDDSDEKGPDSPPSTSA AAAAKKGRRGMKKKVVSVRINGDTP
Sbjct: 1   MDATFNNNRHQFYSGDPLDDSDEKGPDSPPSTSAAAAAAKKGRRGMKKKVVSVRINGDTP 60

Query: 61  RNSSGSTTPPPDSWAWRKY-----------------------------ERNRLDPTMLVI 120
           RNSSGSTTPPPDSWAWRKY                             ERNRLDPTMLVI
Sbjct: 61  RNSSGSTTPPPDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLVI 120

Query: 121 SYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEESLITGD 180
           SYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEESLITGD
Sbjct: 121 SYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEESLITGD 180

Query: 181 EFSWFGEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFADLGELPE 240
           EFSWFGEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFADLGELPE
Sbjct: 181 EFSWFGEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFADLGELPE 240

Query: 241 CSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT 246
           CSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT
Sbjct: 241 CSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT 274

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BLAST of Carg14568 vs. NCBI nr
Match: XP_023541693.1 (probable WRKY transcription factor 65 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 458.4 bits (1178), Expect = 4.0e-125
Identity = 236/274 (86.13%), Postives = 238/274 (86.86%), Query Frame = 0


Query: 1   MDATFNNNRHQFYSGDPLDDSDEKGPDSPPSTSATAAAAKKGRRGMKKKVVSVRINGDTP 60
           MDATFNNNRHQFYSGDP DDSD+K  DSPPSTS   AAAKKGRRGMKKKVVSVRINGDTP
Sbjct: 1   MDATFNNNRHQFYSGDPFDDSDDKALDSPPSTS--TAAAKKGRRGMKKKVVSVRINGDTP 60

Query: 61  RNSSGSTTPPPDSWAWRKY-----------------------------ERNRLDPTMLVI 120
           RNSSGSTTPPPDSWAWRKY                             ERNRLDPTMLVI
Sbjct: 61  RNSSGSTTPPPDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLVI 120

Query: 121 SYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEESLITGD 180
           SYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEESLITGD
Sbjct: 121 SYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEESLITGD 180

Query: 181 EFSWFGEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFADLGELPE 240
           EFSWFGEMETTSSTVLENSIFSGCSSGLGDS+SSDVAMLFPMGDNDVDDSLFADLGELPE
Sbjct: 181 EFSWFGEMETTSSTVLENSIFSGCSSGLGDSLSSDVAMLFPMGDNDVDDSLFADLGELPE 240

Query: 241 CSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT 246
           CSLVFRHGG RGLAADEQPAAGQRRITPWCGTTT
Sbjct: 241 CSLVFRHGGGRGLAADEQPAAGQRRITPWCGTTT 272

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BLAST of Carg14568 vs. NCBI nr
Match: XP_022994861.1 (probable WRKY transcription factor 65 [Cucurbita maxima])

HSP 1 Score: 449.9 bits (1156), Expect = 1.4e-122
Identity = 236/278 (84.89%), Postives = 239/278 (85.97%), Query Frame = 0


Query: 1   MDATFNNNRHQFYSGDPLDDSDEKGPDSPPSTSATAAAAKKGRRGMKKKVVSVRINGDTP 60
           MDATFNNNRHQFYSGDPLDDSDEK PDSPPSTS  AAAAKKGRRGMKKKVVSVRINGD+ 
Sbjct: 1   MDATFNNNRHQFYSGDPLDDSDEKAPDSPPSTS--AAAAKKGRRGMKKKVVSVRINGDSS 60

Query: 61  RNSSGSTTPPPDSWAWRKY-----------------------------ERNRLDPTMLVI 120
           RNSSGSTTPPPDSWAWRKY                             ERNRLDPTMLVI
Sbjct: 61  RNSSGSTTPPPDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLVI 120

Query: 121 SYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEP----EPEVEEKFVDMGEESL 180
           SYSCEHNHSGPVSRNINNHH+AAAV KPGSPESVPVHPEP    E EVEEKFVDMGEESL
Sbjct: 121 SYSCEHNHSGPVSRNINNHHKAAAVGKPGSPESVPVHPEPEVEVEVEVEEKFVDMGEESL 180

Query: 181 ITGDEFSWFGEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFADLG 240
           ITGDEFSWFGEMETTSSTVLENSIFSGCSSGLGDS+SSDVAMLFPMGDNDVDDSLFADLG
Sbjct: 181 ITGDEFSWFGEMETTSSTVLENSIFSGCSSGLGDSLSSDVAMLFPMGDNDVDDSLFADLG 240

Query: 241 ELPECSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT 246
           ELPECSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT
Sbjct: 241 ELPECSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT 276

[Close]

BLAST of Carg14568 vs. NCBI nr
Match: XP_023001002.1 (probable WRKY transcription factor 65 isoform X2 [Cucurbita maxima])

HSP 1 Score: 350.9 bits (899), Expect = 8.9e-93
Identity = 199/278 (71.58%), Postives = 210/278 (75.54%), Query Frame = 0


Query: 2   DATFNNNRHQFYSGDPLDDSDE--KGPDSPPSTSA------TAAAAKKGRRGMKKKVVSV 61
           D TFNNN HQFYS D L+DSDE    PDSPP          + AAAKKGRRGMKKKVVSV
Sbjct: 3   DVTFNNN-HQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVSV 62

Query: 62  RINGDTPRNSSGSTTPPPDSWAWRKY-------------------------ERNRLDPTM 121
           RINGD PRNSSGSTTPP DSWAWRKY                         ERNRLDPTM
Sbjct: 63  RINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRCSSSKGCPARKQVERNRLDPTM 122

Query: 122 LVISYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEESLI 181
           L+I+YSCEHNHSGPVSRN NNHHQAAAV KP SPE+VPVHPEPEPE EEKFV+  E    
Sbjct: 123 LLITYSCEHNHSGPVSRN-NNHHQAAAV-KPTSPETVPVHPEPEPEAEEKFVESLE---- 182

Query: 182 TGDEFSWFGEMETTSSTVLENSIFSG-CSSGLGDSISSDVAMLFPMGDNDVDDSLFADLG 241
             D+FSWFGEMETTSSTVLE+SIFSG  SSG+GDSISSDVAM FPMGD+DVDDSLFADLG
Sbjct: 183 --DQFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLG 242

Query: 242 ELPECSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT 246
           ELPECS+VFRHGG  GL  DEQPA  QRRITPWCG TT
Sbjct: 243 ELPECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 271

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BLAST of Carg14568 vs. ExPASy Swiss-Prot
Match: Q9LP56 (Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 3.7e-33
Identity = 102/242 (42.15%), Postives = 132/242 (54.55%), Query Frame = 0


Query: 19  DDSDEKGPDSPPSTSATAAAA-------KKGRRGMKKKVVSVRINGDTPRNSSGSTTPPP 78
           + S E GP+SP S++     A       K+ RR ++K+VV+V +         G TTPP 
Sbjct: 15  ESSQETGPESPNSSTFNGMKALISSHSPKRSRRSVEKRVVNVPMKEMEGSRHKGDTTPPS 74

Query: 79  DSWAWRKY-----------------------------ERNRLDPTMLVISYSCEHNHSGP 138
           DSWAWRKY                             ER+R DPTM++I+Y+ EHNH  P
Sbjct: 75  DSWAWRKYGQKPIKGSPYPRGYYRCSSTKGCPARKQVERSRDDPTMILITYTSEHNHPWP 134

Query: 139 VSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEE---KFVDMG---EESLITGDEFSWF 198
           ++ +  N  +     KP  PE     PE EPE EE   KF+ +G   E +    DEF+WF
Sbjct: 135 LTSSTRNGPKPKPEPKP-EPE-----PEVEPEAEEEDNKFMVLGRGIETTPSCVDEFAWF 194

Query: 199 GEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFADLGELPECSLVF 219
            EMETTSST+LE+ IFS        S + DVA+ FPMG+   D+SLFADLGELPECS+VF
Sbjct: 195 TEMETTSSTILESPIFSS-EKKTAVSGADDVAVFFPMGEE--DESLFADLGELPECSVVF 247

[Close]

BLAST of Carg14568 vs. ExPASy Swiss-Prot
Match: Q93WV5 (Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.3e-17
Identity = 86/280 (30.71%), Postives = 122/280 (43.57%), Query Frame = 0


Query: 19  DDSDEKGPDSPPSTSATAAAAK------KGRRGMKKKVVSVRINGDTPRNSSGSTTPPPD 78
           D+ DE   D  P + ++   +K      K RR ++K+VVSV I       S G   PP D
Sbjct: 12  DEEDETYNDVVPESPSSCEDSKISKPTPKKRRNVEKRVVSVPIADVEGSKSRGEVYPPSD 71

Query: 79  SWAWRKY-----------------------------ERNRLDPTMLVISYSCEHNHSGP- 138
           SWAWRKY                             ER+R+DP+ L+I+Y+C+HNH  P 
Sbjct: 72  SWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACDHNHPFPS 131

Query: 139 VSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEE------------SLITG 198
            S N  +HH+++ V K    E      E E E EE  V   EE             L+ G
Sbjct: 132 SSANTKSHHRSSVVLKTAKKEE-----EYEEEEEELTVTAAEEPPAGLDLSHVDSPLLLG 191

Query: 199 ------DEFSWFGEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFA 245
                  EF WF +   +S            SSG  + +   +   F +G  + D+SLF 
Sbjct: 192 GCYSEIGEFGWFYDASISS------------SSGSSNFLDVTLERGFSVGQEE-DESLFG 251

[Close]

BLAST of Carg14568 vs. ExPASy Swiss-Prot
Match: Q9SA80 (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 6.6e-06
Identity = 34/95 (35.79%), Postives = 40/95 (42.11%), Query Frame = 0


Query: 41  KGRRGMKKKVVSVRINGDTPRNSSGSTTPPPDSWAWRKY--------------------- 100
           K R+   KKVV +         SSG    P D WAWRKY                     
Sbjct: 188 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPFPRGYYRCSSSK 247

Query: 101 --------ERNRLDPTMLVISYSCEHNHSGPVSRN 107
                   ER+R DP MLVI+Y+ EHNH  P+ RN
Sbjct: 248 GCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRN 281

[Close]

BLAST of Carg14568 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.5e-05
Identity = 34/95 (35.79%), Postives = 39/95 (41.05%), Query Frame = 0


Query: 41  KGRRGMKKKVVSVRINGDTPRNSSGSTTPPPDSWAWRKY--------------------- 100
           K R+   KKVV +         SSG    P D WAWRKY                     
Sbjct: 186 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 245

Query: 101 --------ERNRLDPTMLVISYSCEHNHSGPVSRN 107
                   ER+R DP MLVI+Y+ EHNH  P  RN
Sbjct: 246 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRN 279

[Close]

BLAST of Carg14568 vs. ExPASy TrEMBL
Match: A0A6J1GTP4 (probable WRKY transcription factor 65 OS=Cucurbita moschata OX=3662 GN=LOC111457022 PE=4 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 4.0e-131
Identity = 244/274 (89.05%), Postives = 244/274 (89.05%), Query Frame = 0


Query: 1   MDATFNNNRHQFYSGDPLDDSDEKGPDSPPSTSATAAAAKKGRRGMKKKVVSVRINGDTP 60
           MDATFNNNRHQFYSGDPLDDSDEKGPDSPPSTSA AAAAKKGRRGMKKKVVSVRINGDTP
Sbjct: 1   MDATFNNNRHQFYSGDPLDDSDEKGPDSPPSTSAAAAAAKKGRRGMKKKVVSVRINGDTP 60

Query: 61  RNSSGSTTPPPDSWAWRKY-----------------------------ERNRLDPTMLVI 120
           RNSSGSTTPPPDSWAWRKY                             ERNRLDPTMLVI
Sbjct: 61  RNSSGSTTPPPDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLVI 120

Query: 121 SYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEESLITGD 180
           SYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEESLITGD
Sbjct: 121 SYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEESLITGD 180

Query: 181 EFSWFGEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFADLGELPE 240
           EFSWFGEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFADLGELPE
Sbjct: 181 EFSWFGEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFADLGELPE 240

Query: 241 CSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT 246
           CSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT
Sbjct: 241 CSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT 274

[Close]

BLAST of Carg14568 vs. ExPASy TrEMBL
Match: A0A6J1K0G8 (probable WRKY transcription factor 65 OS=Cucurbita maxima OX=3661 GN=LOC111490461 PE=4 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 6.8e-123
Identity = 236/278 (84.89%), Postives = 239/278 (85.97%), Query Frame = 0


Query: 1   MDATFNNNRHQFYSGDPLDDSDEKGPDSPPSTSATAAAAKKGRRGMKKKVVSVRINGDTP 60
           MDATFNNNRHQFYSGDPLDDSDEK PDSPPSTS  AAAAKKGRRGMKKKVVSVRINGD+ 
Sbjct: 1   MDATFNNNRHQFYSGDPLDDSDEKAPDSPPSTS--AAAAKKGRRGMKKKVVSVRINGDSS 60

Query: 61  RNSSGSTTPPPDSWAWRKY-----------------------------ERNRLDPTMLVI 120
           RNSSGSTTPPPDSWAWRKY                             ERNRLDPTMLVI
Sbjct: 61  RNSSGSTTPPPDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLVI 120

Query: 121 SYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEP----EPEVEEKFVDMGEESL 180
           SYSCEHNHSGPVSRNINNHH+AAAV KPGSPESVPVHPEP    E EVEEKFVDMGEESL
Sbjct: 121 SYSCEHNHSGPVSRNINNHHKAAAVGKPGSPESVPVHPEPEVEVEVEVEEKFVDMGEESL 180

Query: 181 ITGDEFSWFGEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFADLG 240
           ITGDEFSWFGEMETTSSTVLENSIFSGCSSGLGDS+SSDVAMLFPMGDNDVDDSLFADLG
Sbjct: 181 ITGDEFSWFGEMETTSSTVLENSIFSGCSSGLGDSLSSDVAMLFPMGDNDVDDSLFADLG 240

Query: 241 ELPECSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT 246
           ELPECSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT
Sbjct: 241 ELPECSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT 276

[Close]

BLAST of Carg14568 vs. ExPASy TrEMBL
Match: A0A6J1KF84 (probable WRKY transcription factor 65 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495268 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 4.3e-93
Identity = 199/278 (71.58%), Postives = 210/278 (75.54%), Query Frame = 0


Query: 2   DATFNNNRHQFYSGDPLDDSDE--KGPDSPPSTSA------TAAAAKKGRRGMKKKVVSV 61
           D TFNNN HQFYS D L+DSDE    PDSPP          + AAAKKGRRGMKKKVVSV
Sbjct: 3   DVTFNNN-HQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVSV 62

Query: 62  RINGDTPRNSSGSTTPPPDSWAWRKY-------------------------ERNRLDPTM 121
           RINGD PRNSSGSTTPP DSWAWRKY                         ERNRLDPTM
Sbjct: 63  RINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRCSSSKGCPARKQVERNRLDPTM 122

Query: 122 LVISYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEESLI 181
           L+I+YSCEHNHSGPVSRN NNHHQAAAV KP SPE+VPVHPEPEPE EEKFV+  E    
Sbjct: 123 LLITYSCEHNHSGPVSRN-NNHHQAAAV-KPTSPETVPVHPEPEPEAEEKFVESLE---- 182

Query: 182 TGDEFSWFGEMETTSSTVLENSIFSG-CSSGLGDSISSDVAMLFPMGDNDVDDSLFADLG 241
             D+FSWFGEMETTSSTVLE+SIFSG  SSG+GDSISSDVAM FPMGD+DVDDSLFADLG
Sbjct: 183 --DQFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLG 242

Query: 242 ELPECSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT 246
           ELPECS+VFRHGG  GL  DEQPA  QRRITPWCG TT
Sbjct: 243 ELPECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 271

[Close]

BLAST of Carg14568 vs. ExPASy TrEMBL
Match: A0A6J1KJW5 (probable WRKY transcription factor 65 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495268 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 1.3e-92
Identity = 199/282 (70.57%), Postives = 210/282 (74.47%), Query Frame = 0


Query: 2   DATFNNNRHQFYSGDPLDDSDE--KGPDSPPSTSA------TAAAAKKGRRGMKKKVVSV 61
           D TFNNN HQFYS D L+DSDE    PDSPP          + AAAKKGRRGMKKKVVSV
Sbjct: 3   DVTFNNN-HQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVSV 62

Query: 62  RINGDTPRNSSGSTTPPPDSWAWRKY-----------------------------ERNRL 121
           RINGD PRNSSGSTTPP DSWAWRKY                             ERNRL
Sbjct: 63  RINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRL 122

Query: 122 DPTMLVISYSCEHNHSGPVSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGE 181
           DPTML+I+YSCEHNHSGPVSRN NNHHQAAAV KP SPE+VPVHPEPEPE EEKFV+  E
Sbjct: 123 DPTMLLITYSCEHNHSGPVSRN-NNHHQAAAV-KPTSPETVPVHPEPEPEAEEKFVESLE 182

Query: 182 ESLITGDEFSWFGEMETTSSTVLENSIFSG-CSSGLGDSISSDVAMLFPMGDNDVDDSLF 241
                 D+FSWFGEMETTSSTVLE+SIFSG  SSG+GDSISSDVAM FPMGD+DVDDSLF
Sbjct: 183 ------DQFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLF 242

Query: 242 ADLGELPECSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT 246
           ADLGELPECS+VFRHGG  GL  DEQPA  QRRITPWCG TT
Sbjct: 243 ADLGELPECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 275

[Close]

BLAST of Carg14568 vs. ExPASy TrEMBL
Match: E7CEW0 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY6 PE=2 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 1.1e-91
Identity = 199/285 (69.82%), Postives = 214/285 (75.09%), Query Frame = 0


Query: 2   DATFN-NNRHQFYSGDPLDDSDE--KGPDSPPSTSATAAAA--KKGRRGMKKKVVSVRIN 61
           D +FN NN HQFYS DP +DSDE    PDSPP TS  AAAA  KKGRRGMKKK+VSV+IN
Sbjct: 3   DVSFNHNNHHQFYSSDPFEDSDELKPTPDSPPPTSTIAAAATTKKGRRGMKKKIVSVKIN 62

Query: 62  GDTPRNSSGSTTPPPDSWAWRKY-----------------------------ERNRLDPT 121
           GD+PRNSSGS TPP DSWAWRKY                             ERNRLDPT
Sbjct: 63  GDSPRNSSGSATPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPT 122

Query: 122 MLVISYSCEHNHSGPVSRNINNH---HQAAAVEKPGSPESVPVHPEPEPEVEEKFVDM-- 181
            LVI+YSCEHNHSGPVSRN NN+   +    V KPGSPE+V VH   EPEVEEKFV++  
Sbjct: 123 TLVITYSCEHNHSGPVSRNNNNNNNQNNQIVVMKPGSPETVAVH--QEPEVEEKFVEIGG 182

Query: 182 GEESLITGDEFSWFGEMETTSSTVLENSIFSG-CSSGLGD-SISSDVAMLFPMGDNDVDD 241
           GEESLIT DEFSWFGEMETTSSTVLE+SIFSG  S+GL D SISSDVAMLFPMGD+DVD+
Sbjct: 183 GEESLITADEFSWFGEMETTSSTVLESSIFSGRASTGLVDHSISSDVAMLFPMGDDDVDE 242

Query: 242 SLFADLGELPECSLVFRHGGARGLAADEQPAAGQRRITPWCGTTT 246
           SLFADLGELPECSLVFR GG RGL  DEQPAA QRRITPWCGTTT
Sbjct: 243 SLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 285

[Close]

BLAST of Carg14568 vs. TAIR 10
Match: AT1G29280.1 (WRKY DNA-binding protein 65 )

HSP 1 Score: 143.3 bits (360), Expect = 2.6e-34
Identity = 102/242 (42.15%), Postives = 132/242 (54.55%), Query Frame = 0


Query: 19  DDSDEKGPDSPPSTSATAAAA-------KKGRRGMKKKVVSVRINGDTPRNSSGSTTPPP 78
           + S E GP+SP S++     A       K+ RR ++K+VV+V +         G TTPP 
Sbjct: 15  ESSQETGPESPNSSTFNGMKALISSHSPKRSRRSVEKRVVNVPMKEMEGSRHKGDTTPPS 74

Query: 79  DSWAWRKY-----------------------------ERNRLDPTMLVISYSCEHNHSGP 138
           DSWAWRKY                             ER+R DPTM++I+Y+ EHNH  P
Sbjct: 75  DSWAWRKYGQKPIKGSPYPRGYYRCSSTKGCPARKQVERSRDDPTMILITYTSEHNHPWP 134

Query: 139 VSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEE---KFVDMG---EESLITGDEFSWF 198
           ++ +  N  +     KP  PE     PE EPE EE   KF+ +G   E +    DEF+WF
Sbjct: 135 LTSSTRNGPKPKPEPKP-EPE-----PEVEPEAEEEDNKFMVLGRGIETTPSCVDEFAWF 194

Query: 199 GEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFADLGELPECSLVF 219
            EMETTSST+LE+ IFS        S + DVA+ FPMG+   D+SLFADLGELPECS+VF
Sbjct: 195 TEMETTSSTILESPIFSS-EKKTAVSGADDVAVFFPMGEE--DESLFADLGELPECSVVF 247

[Close]

BLAST of Carg14568 vs. TAIR 10
Match: AT3G58710.1 (WRKY DNA-binding protein 69 )

HSP 1 Score: 93.2 bits (230), Expect = 3.1e-19
Identity = 84/274 (30.66%), Postives = 119/274 (43.43%), Query Frame = 0


Query: 19  DDSDEKGPDSPPSTSATAAAAKKGRRGMKKKVVSVRINGDTPRNSSGSTTPPPDSWAWRK 78
           +D   + P S   +  +    KK RR ++K+VVSV I       S G   PP DSWAWRK
Sbjct: 19  NDVVPESPSSCEDSKISKPTPKKSRRNVEKRVVSVPIADVEGSKSRGEVYPPSDSWAWRK 78

Query: 79  Y-----------------------------ERNRLDPTMLVISYSCEHNHSGP-VSRNIN 138
           Y                             ER+R+DP+ L+I+Y+C+HNH  P  S N  
Sbjct: 79  YGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACDHNHPFPSSSANTK 138

Query: 139 NHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEE------------SLITG------ 198
           +HH+++ V K    E      E E E EE  V   EE             L+ G      
Sbjct: 139 SHHRSSVVLKTAKKEE-----EYEEEEEELTVTAAEEPPAGLDLSHVDSPLLLGGCYSEI 198

Query: 199 DEFSWFGEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFADLGELP 245
            EF WF +   +S            SSG  + +   +   F +G  + D+SLF DLG+LP
Sbjct: 199 GEFGWFYDASISS------------SSGSSNFLDVTLERGFSVGQEE-DESLFGDLGDLP 258

[Close]

BLAST of Carg14568 vs. TAIR 10
Match: AT3G58710.2 (WRKY DNA-binding protein 69 )

HSP 1 Score: 91.7 bits (226), Expect = 9.0e-19
Identity = 86/280 (30.71%), Postives = 122/280 (43.57%), Query Frame = 0


Query: 19  DDSDEKGPDSPPSTSATAAAAK------KGRRGMKKKVVSVRINGDTPRNSSGSTTPPPD 78
           D+ DE   D  P + ++   +K      K RR ++K+VVSV I       S G   PP D
Sbjct: 12  DEEDETYNDVVPESPSSCEDSKISKPTPKKRRNVEKRVVSVPIADVEGSKSRGEVYPPSD 71

Query: 79  SWAWRKY-----------------------------ERNRLDPTMLVISYSCEHNHSGP- 138
           SWAWRKY                             ER+R+DP+ L+I+Y+C+HNH  P 
Sbjct: 72  SWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACDHNHPFPS 131

Query: 139 VSRNINNHHQAAAVEKPGSPESVPVHPEPEPEVEEKFVDMGEE------------SLITG 198
            S N  +HH+++ V K    E      E E E EE  V   EE             L+ G
Sbjct: 132 SSANTKSHHRSSVVLKTAKKEE-----EYEEEEEELTVTAAEEPPAGLDLSHVDSPLLLG 191

Query: 199 ------DEFSWFGEMETTSSTVLENSIFSGCSSGLGDSISSDVAMLFPMGDNDVDDSLFA 245
                  EF WF +   +S            SSG  + +   +   F +G  + D+SLF 
Sbjct: 192 GCYSEIGEFGWFYDASISS------------SSGSSNFLDVTLERGFSVGQEE-DESLFG 251

[Close]

BLAST of Carg14568 vs. TAIR 10
Match: AT1G30650.1 (WRKY DNA-binding protein 14 )

HSP 1 Score: 52.8 bits (125), Expect = 4.7e-07
Identity = 34/95 (35.79%), Postives = 40/95 (42.11%), Query Frame = 0


Query: 41  KGRRGMKKKVVSVRINGDTPRNSSGSTTPPPDSWAWRKY--------------------- 100
           K R+   KKVV +         SSG    P D WAWRKY                     
Sbjct: 188 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPFPRGYYRCSSSK 247

Query: 101 --------ERNRLDPTMLVISYSCEHNHSGPVSRN 107
                   ER+R DP MLVI+Y+ EHNH  P+ RN
Sbjct: 248 GCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRN 281

[Close]

BLAST of Carg14568 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 51.6 bits (122), Expect = 1.0e-06
Identity = 34/95 (35.79%), Postives = 39/95 (41.05%), Query Frame = 0


Query: 41  KGRRGMKKKVVSVRINGDTPRNSSGSTTPPPDSWAWRKY--------------------- 100
           K R+   KKVV +         SSG    P D WAWRKY                     
Sbjct: 186 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 245

Query: 101 --------ERNRLDPTMLVISYSCEHNHSGPVSRN 107
                   ER+R DP MLVI+Y+ EHNH  P  RN
Sbjct: 246 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRN 279

The following BLAST results are available for this feature:

BLAST of Carg14568 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012492.1	5.0e-136	100.00	putative WRKY transcription factor 65, partial [Cucurbita argyrosperma subsp. ar...	[more]
XP_022954900.1	8.2e-131	89.05	probable WRKY transcription factor 65 [Cucurbita moschata] >KAG6573324.1 putativ...	[more]
XP_023541693.1	4.0e-125	86.13	probable WRKY transcription factor 65 [Cucurbita pepo subsp. pepo]	[more]
XP_022994861.1	1.4e-122	84.89	probable WRKY transcription factor 65 [Cucurbita maxima]	[more]
XP_023001002.1	8.9e-93	71.58	probable WRKY transcription factor 65 isoform X2 [Cucurbita maxima]	[more]

BLAST of Carg14568 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LP56	3.7e-33	42.15	Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 ...	[more]
Q93WV5	1.3e-17	30.71	Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 ...	[more]
Q9SA80	6.6e-06	35.79	Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 ...	[more]
O64747	1.5e-05	35.79	Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ...	[more]

BLAST of Carg14568 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GTP4	4.0e-131	89.05	probable WRKY transcription factor 65 OS=Cucurbita moschata OX=3662 GN=LOC111457...	[more]
A0A6J1K0G8	6.8e-123	84.89	probable WRKY transcription factor 65 OS=Cucurbita maxima OX=3661 GN=LOC11149046...	[more]
A0A6J1KF84	4.3e-93	71.58	probable WRKY transcription factor 65 isoform X2 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1KJW5	1.3e-92	70.57	probable WRKY transcription factor 65 isoform X1 OS=Cucurbita maxima OX=3661 GN=...	[more]
E7CEW0	1.1e-91	69.82	WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY6 PE=2 SV=1	[more]

BLAST of Carg14568 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G29280.1	2.6e-34	42.15	WRKY DNA-binding protein 65	[more]
AT3G58710.1	3.1e-19	30.66	WRKY DNA-binding protein 69	[more]
AT3G58710.2	9.0e-19	30.71	WRKY DNA-binding protein 69	[more]
AT1G30650.1	4.7e-07	35.79	WRKY DNA-binding protein 14	[more]
AT2G34830.1	1.0e-06	35.79	WRKY DNA-binding protein 35	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..75
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 56..71
None	No IPR available	PANTHER	PTHR32096:SF19	OS01G0750100 PROTEIN	coord: 80..243 coord: 19..79
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR32096	WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED	coord: 80..243
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR32096	WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED	coord: 19..79

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg14568-RA	Carg14568-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0000976	transcription cis-regulatory region binding