Carg14506 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14506
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionGDSL esterase/lipase
LocationCarg_Chr18: 8170666 .. 8171618 (-)
RNA-Seq ExpressionCarg14506
SyntenyCarg14506
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGTGGAGCAAGTTAGAGGCTACATCCCTCAAGTGCTGGACCAGTTCACCACCATTGTCAAGGTTAGCCCTTTGCCCCTTTTTGATCCCCATTTTTAATGGCCTTTTACACAAATGGCCGACCATTTTCGCAGTCGGTGCACGGTCAAGGGGGAAGGTTCTTCTGGATCCATAACACCGGCCCGGTGGGGTGCCTTCCATATGTATTGGTCGGAATGCACGTTTCACTCTCCGACCACGACCAATATGGATGCGCCATTCCTTTCAATGACTTGGCTCAAGAGTTCAACCGCGGCTTGAAGGAAGCTGTGGTTGAGCTTCGCACTGCCTTGCCTGATGCTGTCATCACTTACGTTGATATCTACTCCCTCAAATACGCCATCGTTAGCCGACATAAAAAACATGGTAATTTGTTTGTTTGAATTTTTAGGTTTAGTATGAAGTTTTACGAGTTTGAACTGAAATGTAGGGTTCGAGTTTCCATTGAGAACGTGTTGTGGACATGGTGGAAAGTATAACTTCAATTTGAAGCTTGGATGTGGAGGAACGAAACGGATCCATGGGAAGGATGTTTTGATTGGAAAGGCGTGTAAAAGACCAGGAGTGTATGTGAATTGGGATGGCGTTCATTATACTCAAGCAGCTAACAAATGGATCTTCAATCAGATTAAAACGGGTGCTTTTTCAGACCCACCGACCCCTTTGAAGATGGCTTGTCACCGAACTTAATCAAAGTTTCTTTTTAGCAAAAGCTTCGTGGTTGCTTTAAAATAACTCCCTAATTAGGCGAGACATGCATGTACACATACAACGTGCACCGTGGGACCTTAACCTAATTCATTTTGTTTTTCAATAACCAATCGGACAATTAACCTCGGTTTGCTTTGCCTAATGGCACCCAGTGGAAGTAGTCGAGTATCACTTGTCGTTGTGTTTCATGAAATTATATG

mRNA sequence

ATGAGCGTGGAGCAAGTTAGAGGCTACATCCCTCAAGTGCTGGACCAGTTCACCACCATTGTCAAGTCGGTGCACGGTCAAGGGGGAAGGTTCTTCTGGATCCATAACACCGGCCCGGTGGGGTGCCTTCCATATGTATTGGTCGGAATGCACGTTTCACTCTCCGACCACGACCAATATGGATGCGCCATTCCTTTCAATGACTTGGCTCAAGAGTTCAACCGCGGCTTGAAGGAAGCTGTGGTTGAGCTTCGCACTGCCTTGCCTGATGCTGTCATCACTTACGTTGATATCTACTCCCTCAAATACGCCATCGTTAGCCGACATAAAAAACATGGGTTCGAGTTTCCATTGAGAACGTGTTGTGGACATGGTGGAAAGTATAACTTCAATTTGAAGCTTGGATGTGGAGGAACGAAACGGATCCATGGGAAGGATGTTTTGATTGGAAAGGCGTGTAAAAGACCAGGAGTGTATGTGAATTGGGATGGCGTTCATTATACTCAAGCAGCTAACAAATGGATCTTCAATCAGATTAAAACGGGTGCTTTTTCAGACCCACCGACCCCTTTGAAGATGGCTTGTCACCGAACTTAATCAAAGTTTCTTTTTAGCAAAAGCTTCGTGGTTGCTTTAAAATAACTCCCTAATTAGGCGAGACATGCATGTACACATACAACGTGCACCGTGGGACCTTAACCTAATTCATTTTGTTTTTCAATAACCAATCGGACAATTAACCTCGGTTTGCTTTGCCTAATGGCACCCAGTGGAAGTAGTCGAGTATCACTTGTCGTTGTGTTTCATGAAATTATATG

Coding sequence (CDS)

ATGAGCGTGGAGCAAGTTAGAGGCTACATCCCTCAAGTGCTGGACCAGTTCACCACCATTGTCAAGTCGGTGCACGGTCAAGGGGGAAGGTTCTTCTGGATCCATAACACCGGCCCGGTGGGGTGCCTTCCATATGTATTGGTCGGAATGCACGTTTCACTCTCCGACCACGACCAATATGGATGCGCCATTCCTTTCAATGACTTGGCTCAAGAGTTCAACCGCGGCTTGAAGGAAGCTGTGGTTGAGCTTCGCACTGCCTTGCCTGATGCTGTCATCACTTACGTTGATATCTACTCCCTCAAATACGCCATCGTTAGCCGACATAAAAAACATGGGTTCGAGTTTCCATTGAGAACGTGTTGTGGACATGGTGGAAAGTATAACTTCAATTTGAAGCTTGGATGTGGAGGAACGAAACGGATCCATGGGAAGGATGTTTTGATTGGAAAGGCGTGTAAAAGACCAGGAGTGTATGTGAATTGGGATGGCGTTCATTATACTCAAGCAGCTAACAAATGGATCTTCAATCAGATTAAAACGGGTGCTTTTTCAGACCCACCGACCCCTTTGAAGATGGCTTGTCACCGAACTTAA

Protein sequence

MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQYGCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRTCCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIKTGAFSDPPTPLKMACHRT
Homology
BLAST of Carg14506 vs. NCBI nr
Match: KAG7012777.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 431.8 bits (1109), Expect = 3.2e-117
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY
Sbjct: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT
Sbjct: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120

Query: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180
           CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK
Sbjct: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180

Query: 181 TGAFSDPPTPLKMACHRT 199
           TGAFSDPPTPLKMACHRT
Sbjct: 181 TGAFSDPPTPLKMACHRT 198

BLAST of Carg14506 vs. NCBI nr
Match: KAG6573701.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 421.8 bits (1083), Expect = 3.3e-114
Identity = 193/197 (97.97%), Postives = 196/197 (99.49%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           MSVEQVRGYIPQVL+QFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVS SD+DQY
Sbjct: 127 MSVEQVRGYIPQVLEQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSPSDYDQY 186

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVS+HKKHGFEFPLRT
Sbjct: 187 GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSQHKKHGFEFPLRT 246

Query: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180
           CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK
Sbjct: 247 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 306

Query: 181 TGAFSDPPTPLKMACHR 198
           TGAFSDPPTPLKMACHR
Sbjct: 307 TGAFSDPPTPLKMACHR 323

BLAST of Carg14506 vs. NCBI nr
Match: XP_023541594.1 (GDSL esterase/lipase At3g26430-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 417.9 bits (1073), Expect = 4.8e-113
Identity = 191/198 (96.46%), Postives = 194/198 (97.98%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           MSV QVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVS SD+DQY
Sbjct: 184 MSVAQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSPSDYDQY 243

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GCAIPFNDLAQEFNRGLKEAVVELR ALPDAVITYVDIYSLKYA+VS+HKKHGFEFPLRT
Sbjct: 244 GCAIPFNDLAQEFNRGLKEAVVELRAALPDAVITYVDIYSLKYALVSQHKKHGFEFPLRT 303

Query: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180
           CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK
Sbjct: 304 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 363

Query: 181 TGAFSDPPTPLKMACHRT 199
           TGAFSDPP PLKMACHRT
Sbjct: 364 TGAFSDPPIPLKMACHRT 381

BLAST of Carg14506 vs. NCBI nr
Match: XP_022945912.1 (GDSL esterase/lipase At3g26430-like [Cucurbita moschata])

HSP 1 Score: 415.2 bits (1066), Expect = 3.1e-112
Identity = 190/197 (96.45%), Postives = 192/197 (97.46%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           MSVEQVRGYIPQVLDQ TTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVS SDHDQY
Sbjct: 184 MSVEQVRGYIPQVLDQLTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSPSDHDQY 243

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GCAIPFNDLAQEFNRGLKEAVVELR ALPDAVITYVDIYSLKYA+VS+HKKHGFEFPLRT
Sbjct: 244 GCAIPFNDLAQEFNRGLKEAVVELRAALPDAVITYVDIYSLKYALVSQHKKHGFEFPLRT 303

Query: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180
           CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK
Sbjct: 304 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 363

Query: 181 TGAFSDPPTPLKMACHR 198
           TGAFSDPP PLKMAC R
Sbjct: 364 TGAFSDPPIPLKMACRR 380

BLAST of Carg14506 vs. NCBI nr
Match: KAG7012776.1 (GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 376.7 bits (966), Expect = 1.2e-100
Identity = 168/197 (85.28%), Postives = 184/197 (93.40%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           M+VEQVR YIPQVL QF+TIVK VH QGGRFFWIHNTGPVGCLPYVL  M VS SD+DQY
Sbjct: 185 MTVEQVRDYIPQVLHQFSTIVKWVHNQGGRFFWIHNTGPVGCLPYVLSRMPVSPSDYDQY 244

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GCAIPFNDLAQ FNRGLK+AVVELR ALPDA +TYVD+YSLKYA+VS+HKKHGFEFPLRT
Sbjct: 245 GCAIPFNDLAQTFNRGLKKAVVELRAALPDAALTYVDVYSLKYALVSQHKKHGFEFPLRT 304

Query: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180
           CCGHGGKYNFN+KLGCGGTKR++GKDVLIGKACK+P VYVNWDG+HYT+AANKWIFNQIK
Sbjct: 305 CCGHGGKYNFNVKLGCGGTKRVNGKDVLIGKACKKPEVYVNWDGIHYTEAANKWIFNQIK 364

Query: 181 TGAFSDPPTPLKMACHR 198
           TGA+SDPP PL+MACHR
Sbjct: 365 TGAYSDPPIPLEMACHR 381

BLAST of Carg14506 vs. ExPASy Swiss-Prot
Match: Q9LIN2 (GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 4.0e-62
Identity = 101/197 (51.27%), Postives = 136/197 (69.04%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           M+ +Q++ YIP V DQ + +++ V+ +GGR FWIHNT P+GCLPYVL    V  S  D +
Sbjct: 184 MTSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPASQIDNH 243

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GCAIP N++A+ +N  LK  V+ELR  L +A  TYVDIYS+K  ++++ KK GF +PL  
Sbjct: 244 GCAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGFRYPLVA 303

Query: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180
           CCGHGGKYNFN  + CG    I GK++++ K+C      V+WDG+H+T+  N WIF QI 
Sbjct: 304 CCGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIFQQIN 363

Query: 181 TGAFSDPPTPLKMACHR 198
            GAFSDPP P+K AC R
Sbjct: 364 DGAFSDPPLPVKSACTR 380

BLAST of Carg14506 vs. ExPASy Swiss-Prot
Match: B4FZ87 (GDSL esterase/lipase ACHE OS=Zea mays OX=4577 GN=ACHE PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.3e-60
Identity = 99/196 (50.51%), Postives = 141/196 (71.94%), Query Frame = 0

Query: 2   SVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQYG 61
           + E+V   IP ++++ T+I++SV+ +GGR+FWIHNTGP+GCLPY L+         D  G
Sbjct: 197 TTEEVEAIIPDLMERLTSIIQSVYSRGGRYFWIHNTGPLGCLPYALLHRPDLAIPADGTG 256

Query: 62  CAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRTC 121
           C++ +N +AQ FN  LKE V  LR   PDA  TYVD+Y+ KY ++S+  K GF+ PL TC
Sbjct: 257 CSVTYNKVAQLFNLRLKETVASLRKTHPDAAFTYVDVYTAKYKLISQANKLGFDDPLLTC 316

Query: 122 CGH-GGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 181
           CG+ GG+YN +L +GCGG K+++G  V++GK+C+ P   V+WDGVH+T+AANK++F+QI 
Sbjct: 317 CGYGGGRYNLDLSVGCGGKKQVNGTSVVVGKSCENPSKRVSWDGVHFTEAANKFVFDQIV 376

Query: 182 TGAFSDPPTPLKMACH 197
            GA SDPP  L+ ACH
Sbjct: 377 AGALSDPPVALRQACH 392

BLAST of Carg14506 vs. ExPASy Swiss-Prot
Match: Q7Y1X1 (Esterase OS=Hevea brasiliensis OX=3981 PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 5.4e-59
Identity = 100/198 (50.51%), Postives = 136/198 (68.69%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           ++VE+V   +P +++ F+  VK ++  G R FWIHNTGP+GCL ++L   +   ++ D  
Sbjct: 187 LTVEEVNATVPDLVNSFSANVKKIYDLGARTFWIHNTGPIGCLSFILT--YFPWAEKDSA 246

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GCA  +N++AQ FN  LKE V +LR  LP A   +VDIYS+KY++ S  +KHGFEFPL T
Sbjct: 247 GCAKAYNEVAQHFNHKLKEIVAQLRKDLPLATFVHVDIYSVKYSLFSEPEKHGFEFPLIT 306

Query: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180
           CCG+GGKYNF++   CG T        ++  +C  P V VNWDG HYT+AAN++ F+QI 
Sbjct: 307 CCGYGGKYNFSVTAPCGDTVTADDGTKIVVGSCACPSVRVNWDGAHYTEAANEYFFDQIS 366

Query: 181 TGAFSDPPTPLKMACHRT 199
           TGAFSDPP PL MACH+T
Sbjct: 367 TGAFSDPPVPLNMACHKT 382

BLAST of Carg14506 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 5.4e-59
Identity = 102/196 (52.04%), Postives = 135/196 (68.88%), Query Frame = 0

Query: 2   SVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQYG 61
           +VEQV   +P+++ QF   +K+++GQGGR+FWIHNTGP+GCL YV+       SD D +G
Sbjct: 184 TVEQVETEVPEIISQFMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHG 243

Query: 62  CAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRTC 121
           C  P N LAQ+FN  LK+AV+ELR++L +A ITYVD+YSLK+ +    + HGF+  L +C
Sbjct: 244 CVSPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSC 303

Query: 122 CGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIKT 181
           CGHGGKYN+N  +GCG  K + GK+V IGK C  P   V WDGVH+TQAANK+IF++I  
Sbjct: 304 CGHGGKYNYNKGIGCGMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAP 363

Query: 182 GAFSDPPTPLKMACHR 198
           G        L  AC R
Sbjct: 364 G--------LSKACKR 371

BLAST of Carg14506 vs. ExPASy Swiss-Prot
Match: Q9LY84 (GDSL esterase/lipase At5g14450 OS=Arabidopsis thaliana OX=3702 GN=At5g14450 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 1.8e-46
Identity = 85/198 (42.93%), Postives = 126/198 (63.64%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDH-DQ 60
           MSV+Q++  IP +++   + V++++ QGGR FW+HNTGP GCLP  +  M      + D+
Sbjct: 195 MSVDQLKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDK 254

Query: 61  YGCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLR 120
            GC    N++A EFNR LKE V+ LR  L  A ITYVD+Y+ KY ++S  KK GF  PL+
Sbjct: 255 SGCVKAQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFANPLK 314

Query: 121 TCCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQI 180
            CCG+  KY+    + CG   +++  ++  G +C  P + V+WDGVHYT+AANK + ++ 
Sbjct: 315 VCCGYHEKYD---HIWCGNKGKVNNTEI-YGGSCPNPVMAVSWDGVHYTEAANKHVADRT 374

Query: 181 KTGAFSDPPTPLKMACHR 198
             G  +DPP P+  AC+R
Sbjct: 375 LNGLLTDPPVPITRACYR 388

BLAST of Carg14506 vs. ExPASy TrEMBL
Match: A0A6J1G281 (GDSL esterase/lipase At3g26430-like OS=Cucurbita moschata OX=3662 GN=LOC111450015 PE=3 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 1.5e-112
Identity = 190/197 (96.45%), Postives = 192/197 (97.46%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           MSVEQVRGYIPQVLDQ TTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVS SDHDQY
Sbjct: 184 MSVEQVRGYIPQVLDQLTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSPSDHDQY 243

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GCAIPFNDLAQEFNRGLKEAVVELR ALPDAVITYVDIYSLKYA+VS+HKKHGFEFPLRT
Sbjct: 244 GCAIPFNDLAQEFNRGLKEAVVELRAALPDAVITYVDIYSLKYALVSQHKKHGFEFPLRT 303

Query: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180
           CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK
Sbjct: 304 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 363

Query: 181 TGAFSDPPTPLKMACHR 198
           TGAFSDPP PLKMAC R
Sbjct: 364 TGAFSDPPIPLKMACRR 380

BLAST of Carg14506 vs. ExPASy TrEMBL
Match: A0A6J1G274 (GDSL esterase/lipase At3g26430-like OS=Cucurbita moschata OX=3662 GN=LOC111450014 PE=3 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.1e-99
Identity = 166/197 (84.26%), Postives = 183/197 (92.89%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           M+VEQVR YIPQVL QF+TIVK VH QGGRFFWIHNTGPVGCLPYVL  M VS SD+DQY
Sbjct: 185 MTVEQVRDYIPQVLHQFSTIVKWVHSQGGRFFWIHNTGPVGCLPYVLSRMPVSPSDYDQY 244

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GCAIPFNDLAQ FNRGLK+AVVELR ALPDA +TYVD+YSLKYA+VS+HKKHGFEFPLRT
Sbjct: 245 GCAIPFNDLAQTFNRGLKKAVVELRAALPDAALTYVDVYSLKYALVSQHKKHGFEFPLRT 304

Query: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180
           CCGHGGKYNFN+KLGCGGTKR++G DVLIGKACK+P VYVNWDG+HYT+AANKWIFNQIK
Sbjct: 305 CCGHGGKYNFNVKLGCGGTKRVNGNDVLIGKACKKPEVYVNWDGIHYTEAANKWIFNQIK 364

Query: 181 TGAFSDPPTPLKMACHR 198
           TGA+SDPP PL+MAC+R
Sbjct: 365 TGAYSDPPIPLEMACYR 381

BLAST of Carg14506 vs. ExPASy TrEMBL
Match: A0A6J1HP45 (GDSL esterase/lipase At3g26430 OS=Cucurbita maxima OX=3661 GN=LOC111466427 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.7e-98
Identity = 166/197 (84.26%), Postives = 181/197 (91.88%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           M+VEQVR YIPQVLDQF+TIVK VH QGGRFFWIHNTGPVGCLPYVL  M VS SD+DQY
Sbjct: 184 MTVEQVRDYIPQVLDQFSTIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRMPVSPSDYDQY 243

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GCAIPFNDLAQ FNRGLK+AVVELR ALPDA +TYVDIYSLKY +VS+HKK+GFEFPLRT
Sbjct: 244 GCAIPFNDLAQTFNRGLKDAVVELRAALPDAALTYVDIYSLKYDLVSQHKKYGFEFPLRT 303

Query: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180
           CCGHGGKYNFN+KLGC G KR++GKDVLIGKAC++P VYVNWDGVHYTQAANKWIFN IK
Sbjct: 304 CCGHGGKYNFNVKLGCAGKKRMNGKDVLIGKACEKPEVYVNWDGVHYTQAANKWIFNHIK 363

Query: 181 TGAFSDPPTPLKMACHR 198
           TGA+SDPP PL+MACHR
Sbjct: 364 TGAYSDPPIPLEMACHR 380

BLAST of Carg14506 vs. ExPASy TrEMBL
Match: A0A6J1HQE7 (GDSL esterase/lipase At3g26430-like OS=Cucurbita maxima OX=3661 GN=LOC111466426 PE=3 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 1.0e-97
Identity = 162/197 (82.23%), Postives = 180/197 (91.37%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           M+VEQVR YIPQVL QF+TIVK VH QGGRFFWIHNTGPVGCLPYVL  M VS SD+D+Y
Sbjct: 185 MTVEQVRDYIPQVLHQFSTIVKWVHSQGGRFFWIHNTGPVGCLPYVLSRMPVSPSDYDRY 244

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GCAIPFNDLAQ FN GLK+ V ELR ALPDA +TYVD+YSLKY +VS+HKKHGFEFPLRT
Sbjct: 245 GCAIPFNDLAQTFNCGLKKVVAELRAALPDATLTYVDVYSLKYTLVSQHKKHGFEFPLRT 304

Query: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180
           CCGHGGKYNFN+KLGCGGTKR++GKDVLIGKACK+P VYVNWDG+HYT+AANKWIFN+IK
Sbjct: 305 CCGHGGKYNFNVKLGCGGTKRVNGKDVLIGKACKKPEVYVNWDGIHYTEAANKWIFNKIK 364

Query: 181 TGAFSDPPTPLKMACHR 198
           TGA+SDPP PL+MACHR
Sbjct: 365 TGAYSDPPIPLEMACHR 381

BLAST of Carg14506 vs. ExPASy TrEMBL
Match: A0A6J1G253 (alpha-L-fucosidase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111449990 PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.5e-96
Identity = 163/197 (82.74%), Postives = 180/197 (91.37%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           M+VEQVR YIPQVL+QF+TIVK VH QGGRFFWIHNTGPVGCLPYVL  M VS SD+DQY
Sbjct: 184 MTVEQVRDYIPQVLNQFSTIVKWVHSQGGRFFWIHNTGPVGCLPYVLDRMPVSPSDYDQY 243

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GCAIPFNDLAQ FN GLK+AVV+LR ALP+A +TYVDIYSLKY +VS+HKKHGFEFPLRT
Sbjct: 244 GCAIPFNDLAQTFNHGLKDAVVKLRAALPNATLTYVDIYSLKYDLVSQHKKHGFEFPLRT 303

Query: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180
           CCGHGGKYNFN+KLGC G KR++GKDVLIGKACK+P VYVNWDGVHYTQAAN WIFNQIK
Sbjct: 304 CCGHGGKYNFNVKLGCAGKKRMNGKDVLIGKACKKPEVYVNWDGVHYTQAANNWIFNQIK 363

Query: 181 TGAFSDPPTPLKMACHR 198
           TGA+SDPP PL+MAC+R
Sbjct: 364 TGAYSDPPIPLEMACYR 380

BLAST of Carg14506 vs. TAIR 10
Match: AT3G26430.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 239.2 bits (609), Expect = 2.8e-63
Identity = 101/197 (51.27%), Postives = 136/197 (69.04%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           M+ +Q++ YIP V DQ + +++ V+ +GGR FWIHNT P+GCLPYVL    V  S  D +
Sbjct: 184 MTSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPASQIDNH 243

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GCAIP N++A+ +N  LK  V+ELR  L +A  TYVDIYS+K  ++++ KK GF +PL  
Sbjct: 244 GCAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGFRYPLVA 303

Query: 121 CCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 180
           CCGHGGKYNFN  + CG    I GK++++ K+C      V+WDG+H+T+  N WIF QI 
Sbjct: 304 CCGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIFQQIN 363

Query: 181 TGAFSDPPTPLKMACHR 198
            GAFSDPP P+K AC R
Sbjct: 364 DGAFSDPPLPVKSACTR 380

BLAST of Carg14506 vs. TAIR 10
Match: AT1G67830.1 (alpha-fucosidase 1 )

HSP 1 Score: 228.8 bits (582), Expect = 3.8e-60
Identity = 102/196 (52.04%), Postives = 135/196 (68.88%), Query Frame = 0

Query: 2   SVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQYG 61
           +VEQV   +P+++ QF   +K+++GQGGR+FWIHNTGP+GCL YV+       SD D +G
Sbjct: 184 TVEQVETEVPEIISQFMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHG 243

Query: 62  CAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRTC 121
           C  P N LAQ+FN  LK+AV+ELR++L +A ITYVD+YSLK+ +    + HGF+  L +C
Sbjct: 244 CVSPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSC 303

Query: 122 CGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIKT 181
           CGHGGKYN+N  +GCG  K + GK+V IGK C  P   V WDGVH+TQAANK+IF++I  
Sbjct: 304 CGHGGKYNYNKGIGCGMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAP 363

Query: 182 GAFSDPPTPLKMACHR 198
           G        L  AC R
Sbjct: 364 G--------LSKACKR 371

BLAST of Carg14506 vs. TAIR 10
Match: AT5G14450.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 187.2 bits (474), Expect = 1.3e-47
Identity = 85/198 (42.93%), Postives = 126/198 (63.64%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDH-DQ 60
           MSV+Q++  IP +++   + V++++ QGGR FW+HNTGP GCLP  +  M      + D+
Sbjct: 195 MSVDQLKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDK 254

Query: 61  YGCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLR 120
            GC    N++A EFNR LKE V+ LR  L  A ITYVD+Y+ KY ++S  KK GF  PL+
Sbjct: 255 SGCVKAQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFANPLK 314

Query: 121 TCCGHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQI 180
            CCG+  KY+    + CG   +++  ++  G +C  P + V+WDGVHYT+AANK + ++ 
Sbjct: 315 VCCGYHEKYD---HIWCGNKGKVNNTEI-YGGSCPNPVMAVSWDGVHYTEAANKHVADRT 374

Query: 181 KTGAFSDPPTPLKMACHR 198
             G  +DPP P+  AC+R
Sbjct: 375 LNGLLTDPPVPITRACYR 388

BLAST of Carg14506 vs. TAIR 10
Match: AT1G56670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 175.6 bits (444), Expect = 3.9e-44
Identity = 81/197 (41.12%), Postives = 120/197 (60.91%), Query Frame = 0

Query: 2   SVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQYG 61
           S  Q    IPQ++ +  + +K ++ +GGR FWIHNTGP+GCLP  L    V   D DQ+G
Sbjct: 188 SYSQTVKLIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKL--SMVKSKDLDQHG 247

Query: 62  CAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRTC 121
           C + +N  A  FN+GL     ELRT L DA I Y+DIY++KY++++   ++GF+ PL  C
Sbjct: 248 CLVSYNSAATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQYGFKSPLMAC 307

Query: 122 CGHGG-KYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQIK 181
           CG+GG  YN+N+K+ CG      G +V     C+    +++WDG+HYT+ AN  +  ++ 
Sbjct: 308 CGYGGTPYNYNVKITCGH----KGSNV-----CEEGSRFISWDGIHYTETANAIVAMKVL 367

Query: 182 TGAFSDPPTPLKMACHR 198
           +  +S PPTP    C R
Sbjct: 368 SMHYSKPPTPFHFFCRR 373

BLAST of Carg14506 vs. TAIR 10
Match: AT3G27950.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 168.7 bits (426), Expect = 4.7e-42
Identity = 81/199 (40.70%), Postives = 120/199 (60.30%), Query Frame = 0

Query: 1   MSVEQVRGYIPQVLDQFTTIVKSVHGQGGRFFWIHNTGPVGCLPYVLVGMHVSLSDHDQY 60
           M+ EQ++  IP +++ FT  +K ++ +G RFF IHNTGP GCLPY+L          D Y
Sbjct: 169 MTEEQLKATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLPYLLKAF--PAIPRDPY 228

Query: 61  GCAIPFNDLAQEFNRGLKEAVVELRTALPDAVITYVDIYSLKYAIVSRHKKHGFEFPLRT 120
           GC  P N++A EFN+ LK  + +L+  LP +  TYVD+YS KY ++++ K  GF  P   
Sbjct: 229 GCLKPLNNVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDPFDY 288

Query: 121 CC-GHGGKYNFNLKLGCGGTKRIHGKDVLIGKACKRPGVYVNWDGVHYTQAANKWIFNQI 180
           CC G  G+      +GCG T  ++G + L   +C+    +++WDG+HYT+ AN  + N+I
Sbjct: 289 CCVGAIGR-----GMGCGKTIFLNGTE-LYSSSCQNRKNFISWDGIHYTETANMLVANRI 348

Query: 181 KTGAFSDPPTPLKMACHRT 199
             G+ SDPP P + AC  T
Sbjct: 349 LDGSISDPPLPTQKACKLT 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7012777.13.2e-117100.00GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6573701.13.3e-11497.97GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023541594.14.8e-11396.46GDSL esterase/lipase At3g26430-like [Cucurbita pepo subsp. pepo][more]
XP_022945912.13.1e-11296.45GDSL esterase/lipase At3g26430-like [Cucurbita moschata][more]
KAG7012776.11.2e-10085.28GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9LIN24.0e-6251.27GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1... [more]
B4FZ871.3e-6050.51GDSL esterase/lipase ACHE OS=Zea mays OX=4577 GN=ACHE PE=1 SV=1[more]
Q7Y1X15.4e-5950.51Esterase OS=Hevea brasiliensis OX=3981 PE=1 SV=1[more]
Q9FXE55.4e-5952.04Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1[more]
Q9LY841.8e-4642.93GDSL esterase/lipase At5g14450 OS=Arabidopsis thaliana OX=3702 GN=At5g14450 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1G2811.5e-11296.45GDSL esterase/lipase At3g26430-like OS=Cucurbita moschata OX=3662 GN=LOC11145001... [more]
A0A6J1G2741.1e-9984.26GDSL esterase/lipase At3g26430-like OS=Cucurbita moschata OX=3662 GN=LOC11145001... [more]
A0A6J1HP452.7e-9884.26GDSL esterase/lipase At3g26430 OS=Cucurbita maxima OX=3661 GN=LOC111466427 PE=3 ... [more]
A0A6J1HQE71.0e-9782.23GDSL esterase/lipase At3g26430-like OS=Cucurbita maxima OX=3661 GN=LOC111466426 ... [more]
A0A6J1G2531.5e-9682.74alpha-L-fucosidase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111449990 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G26430.12.8e-6351.27GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G67830.13.8e-6052.04alpha-fucosidase 1 [more]
AT5G14450.11.3e-4742.93GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G56670.13.9e-4441.12GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G27950.14.7e-4240.70GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 10..179
e-value: 1.7E-23
score: 84.3
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 2..182
e-value: 3.6E-37
score: 130.5
NoneNo IPR availablePANTHERPTHR22835:SF117BNAA06G32900D PROTEINcoord: 3..197
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 3..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14506-RACarg14506-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds