Carg14476 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14476
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPectin lyase-like superfamily protein
LocationCarg_Chr18: 8395486 .. 8398709 (+)
RNA-Seq ExpressionCarg14476
SyntenyCarg14476
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAAGTTCTTCCTGCTTCCCCTGCTTTCCCTGCTGATCATAGCCTATGGAGTTTCCGGTAGCATCGACCCTGAAAGCGACATCGACATGGTTAAAGAGCTTGAAACTTTTGACATTGAAGAACAGGACGACAATGTGGTGGACACATACGCAATTCCAGTGTGGAAAAGCGAGCGTGGGAGTCGGTCTCTAATGAATGTGGATAGTTTTGACGCCGTTGGAGACGGAATTACAGATGATACCATGGTAGTACATAAACATTTCCATTTTGGAAACATGATTTTGGCAGAGATGATACTAAACCAAAGTGTTTATTTTGCTATCAGGCTTTCAGGAAGGCATGGGAAACTGCCTGCAGCGCTGCAAAATCAGTTCTTTTGGTGCCAAAAGAAAAGACATATCTGGTGAATGCTACCACTTTTAAAGGGCCATGCAATGATGGTATGATAATCCAGGTAAAACACAAGATTATGCAAGGATTTCTTTGTTTTTTTTTTTTTTCTCCAAATAAATGGGAAGATCATGGTTAAAGAACAGAGCTAACCTTGCAGATTGAAGGAACAATAATTGCACCAGATGAACCAGAGAAATGGGATCCAAAACTTCCACGACAATGGCTGGAATTCACAAAACTTAATGCAGCTACATTTCAAGGTGATGGTGTTATCGATGGCTCCGGTGAGAAATGGTGGGCAGAATCCTGCAAAAAAAACAAGTCCAGGGTAAATTTTGAGCTGTTCCAGTGCTTGTTTCTTAGACTATGTAATTCAAATGCTGATCATTTCTGGTTTTTTTTTTTACAGCCCTGCAAAGGAGCACCAACAGTAGGGACAAAACCACCTCAAAAACCTTATCTTTCCAAGTGCAACACATTCTGATTTGATGTTTTTTTCTTGCAGGCATTAACCATTGATTCAAGTTCAAATATAAGAGTAAAAGGCCTTACTATTCAAAACAGCCAACAGATGCATTTCACCATTGCACGATCTGAAACTGTTCGGATTACGGAGGTACGAGTCTCGTCTCCAGGAGACAGCCCGAACACGGACGGGATCCACATCACTCAATCAACGAATGTCGTAGTCCAAAACTGCAAGATTTCTACAGGTTTAACCCCAATGTTTTCCCGCTCTAAACTGGCTGGAAAGTGTATGGATTAAGAATGGTTGTGTAATGAATGTGTAGGTGATGATTGCATATCCATTGTCAATGCTAGCTCTGGCATCAAAATGAAAGGGATATCATGTGGACCAGGACATGGGATCAGGTATTAACAAATGGTTTCATTTCATTCAGCTCCAAAACAAGATCAATGTCTTAGAATTCGATTCTCTTTGTTTGTTTGCTGTGAAACTGTGTTCCTCAGCATTGGAAGTTTAGGGAAAGACAACTCTGCTGGGATTGTGTCAAAGGTGGTGCTAGACACTGCCTTTCTCCGAGAGACAACAAATGGTGTTAGAATCAAGACATGGCAGGTTAGAATCACAGCTTTAACCACAATTTTGATACTACTTTTACACGGTCATATGGTGTGAGAACCCCACATTGGTTGGAGAGGAGAACGAAACATCCTTTATAAGGGTGTGGAAACCTTTCCCTAGGAAACACGTTTTAAAGCCTTGAGAGGAAGTCTGAAAGGGAAAGTCCAAAAAGGGCAGTATCTCCTAGTGGTGGGCTTGGGCCGTTACAAATGGTATCAGAGCCAGACACCGGACTATGTGCCAGCGAAGAGGTTGTTCCCCGAAGGGGGGTAAACATGAGGCGGTGTGCTAGTAAGGACACTGGGCTCCGAAGGGAGGTGGATTGTGAGAATCTCACATTAGTTGGAGAGGAGAACAAAACACCCTTTATAAGGGTGTGGAAACCTTTCCCTAGGAGACACGTTTTAAAGCCTTGAGGGGAAGCCCGAAAAGGAAAACCCATAGAGGACAATATCTGCTAGCGGTGGGTCTTGACCGTTACATATGGGCAATTATAACCAATAATAATGGGGAAACTACTATGTTCATGTGGCTATAGGGAGGTTCGGGGTATGTTCGTGCCGTACGATTTGAAAACGTGAGAATGCAAGATGTTGAGAACCCAATTATCATAGATCAATTCTACTGTGACTCTCCTAATGCTTGTGAAACTCAGGTATTATCCACAAAATCCATATTCAGCTACTAATCAATAAGACAAAGTTTGGCTAATGCCTTTTGTTTTTGTTAGACATCTGCCATTAAAGTAAGCCAAATCATGTACCGGAACATAAGTGGAACCACAACCAGCAAGAACGCAATGAAATTCGCCTGTAGCGACAGTGTTCCATGCAGCAACATAATTCTAAGCAATGTCAATTTGGAGAAGACGGATGGCACAGTGGAGACCTATTGCCATGCTGCTCAAGGCTTCGGATATGGCGTCGTGCACCCATCGGCCGACTGCCTAACCTCCAGCGACAATGACTTTGCTGCTAAAGATCAAACCCAACTCCCTACTGAGCAGCACATTGTTCACACTGAACTCTGACCTTGTTATCCTGTCTATCTGTACTAAACTACTAAAAGGATCATAATTTTACATAGCATAGACAATTATACCTACCCTTTTGGAATCTAAATAGAAATATAGATTACTAAGTAAAGGATTGTACTACTGCTAGAAGAAAATATACATATTTTGAGTGTATAGTAAAGTTTTTTTTTTAATACTTAAGTTCATATCATATGGTTGATTGTTCTCTGAACTATAGGTAGTATATACATTACTCCAAGAAAGAACTATATAATATACAAAAATGTGAAGATGGGTAGTAGTGGACAATGATGAGTACAAATTAAGGGGAAGATTGAGGAGGAAGGAACATTGTTAATGGAGTACATTGGACGCCCAGCAAACAAATCAAAGTTGAGTCTTCCTTTCTGAACATTCTTAATGTGGATACCAAAAAATGAAGTGAAAACAGAGCGGTTTCTGGTGGAATTTCAACTTCATGAAAGGGAAAGATGGCGTACGGTAGAAGTCATGAAACAAGACAAAGTGGTCCAGTGGAGTAATTTTATTGTATCAAAATGGCACTTCCACTAAGTGATGGTGGATGAAATTGTGAGACTGTACATCAGAGACTGTACATCAGTCGGAGATCGAAATGAAACATTGAAACATTTTTTATAAGGGTGTGGAAACCTCTCCCTAATAGACGCGTTTTAAAACCGTGAGGCTGACGGTGATACGTAACG

mRNA sequence

ATGGACAAGTTCTTCCTGCTTCCCCTGCTTTCCCTGCTGATCATAGCCTATGGAGTTTCCGGTAGCATCGACCCTGAAAGCGACATCGACATGGTTAAAGAGCTTGAAACTTTTGACATTGAAGAACAGGACGACAATGTGGTGGACACATACGCAATTCCAGTGTGGAAAAGCGAGCGTGGGAGTCGGTCTCTAATGAATGTGGATAGTTTTGACGCCGTTGGAGACGGAATTACAGATGATACCATGGCTTTCAGGAAGGCATGGGAAACTGCCTGCAGCGCTGCAAAATCAGTTCTTTTGGTGCCAAAAGAAAAGACATATCTGGTGAATGCTACCACTTTTAAAGGGCCATGCAATGATGGTATGATAATCCAGATTGAAGGAACAATAATTGCACCAGATGAACCAGAGAAATGGGATCCAAAACTTCCACGACAATGGCTGGAATTCACAAAACTTAATGCAGCTACATTTCAAGGTGATGGTGTTATCGATGGCTCCGGTGAGAAATGGTGGGCAGAATCCTGCAAAAAAAACAAGTCCAGGCCCTGCAAAGGAGCACCAACAGCATTAACCATTGATTCAAGTTCAAATATAAGAGTAAAAGGCCTTACTATTCAAAACAGCCAACAGATGCATTTCACCATTGCACGATCTGAAACTGTTCGGATTACGGAGGTACGAGTCTCGTCTCCAGGAGACAGCCCGAACACGGACGGGATCCACATCACTCAATCAACGAATGTCGTAGTCCAAAACTGCAAGATTTCTACAGGTGATGATTGCATATCCATTGTCAATGCTAGCTCTGGCATCAAAATGAAAGGGATATCATGTGGACCAGGACATGGGATCAGCATTGGAAGTTTAGGGAAAGACAACTCTGCTGGGATTGTGTCAAAGGTGGTGCTAGACACTGCCTTTCTCCGAGAGACAACAAATGGTGTTAGAATCAAGACATGGCAGGGAGGTTCGGGGTATGTTCGTGCCGTACGATTTGAAAACGTGAGAATGCAAGATGTTGAGAACCCAATTATCATAGATCAATTCTACTGTGACTCTCCTAATGCTTGTGAAACTCAGACATCTGCCATTAAAGTAAGCCAAATCATGTACCGGAACATAAGTGGAACCACAACCAGCAAGAACGCAATGAAATTCGCCTGTAGCGACAGTGTTCCATGCAGCAACATAATTCTAAGCAATGTCAATTTGGAGAAGACGGATGGCACAGTGGAGACCTATTGCCATGCTGCTCAAGGCTTCGGATATGGCGTCGTGCACCCATCGGCCGACTGCCTAACCTCCAGCGACAATGACTTTGCTGCTAAAGATCAAACCCAACTCCCTACTGAGCAGCACATTGTTCACACTGAACTCTGACCTTGTTATCCTGTCTATCTGTACTAAACTACTAAAAGGATCATAATTTTACATAGCATAGACAATTATACCTACCCTTTTGGAATCTAAATAGAAATATAGATTACTAAGTAAAGGATTGTACTACTGCTAGAAGAAAATATACATATTTTGAGTGTATAGTAAAGTTTTTTTTTTAATACTTAAGTTCATATCATATGGTTGATTGTTCTCTGAACTATAGGTAGTATATACATTACTCCAAGAAAGAACTATATAATATACAAAAATGTGAAGATGGGTAGTAGTGGACAATGATGAGTACAAATTAAGGGGAAGATTGAGGAGGAAGGAACATTGTTAATGGAGTACATTGGACGCCCAGCAAACAAATCAAAGTTGAGTCTTCCTTTCTGAACATTCTTAATGTGGATACCAAAAAATGAAGTGAAAACAGAGCGGTTTCTGGTGGAATTTCAACTTCATGAAAGGGAAAGATGGCGTACGGTAGAAGTCATGAAACAAGACAAAGTGGTCCAGTGGAGTAATTTTATTGTATCAAAATGGCACTTCCACTAAGTGATGGTGGATGAAATTGTGAGACTGTACATCAGAGACTGTACATCAGTCGGAGATCGAAATGAAACATTGAAACATTTTTTATAAGGGTGTGGAAACCTCTCCCTAATAGACGCGTTTTAAAACCGTGAGGCTGACGGTGATACGTAACG

Coding sequence (CDS)

ATGGACAAGTTCTTCCTGCTTCCCCTGCTTTCCCTGCTGATCATAGCCTATGGAGTTTCCGGTAGCATCGACCCTGAAAGCGACATCGACATGGTTAAAGAGCTTGAAACTTTTGACATTGAAGAACAGGACGACAATGTGGTGGACACATACGCAATTCCAGTGTGGAAAAGCGAGCGTGGGAGTCGGTCTCTAATGAATGTGGATAGTTTTGACGCCGTTGGAGACGGAATTACAGATGATACCATGGCTTTCAGGAAGGCATGGGAAACTGCCTGCAGCGCTGCAAAATCAGTTCTTTTGGTGCCAAAAGAAAAGACATATCTGGTGAATGCTACCACTTTTAAAGGGCCATGCAATGATGGTATGATAATCCAGATTGAAGGAACAATAATTGCACCAGATGAACCAGAGAAATGGGATCCAAAACTTCCACGACAATGGCTGGAATTCACAAAACTTAATGCAGCTACATTTCAAGGTGATGGTGTTATCGATGGCTCCGGTGAGAAATGGTGGGCAGAATCCTGCAAAAAAAACAAGTCCAGGCCCTGCAAAGGAGCACCAACAGCATTAACCATTGATTCAAGTTCAAATATAAGAGTAAAAGGCCTTACTATTCAAAACAGCCAACAGATGCATTTCACCATTGCACGATCTGAAACTGTTCGGATTACGGAGGTACGAGTCTCGTCTCCAGGAGACAGCCCGAACACGGACGGGATCCACATCACTCAATCAACGAATGTCGTAGTCCAAAACTGCAAGATTTCTACAGGTGATGATTGCATATCCATTGTCAATGCTAGCTCTGGCATCAAAATGAAAGGGATATCATGTGGACCAGGACATGGGATCAGCATTGGAAGTTTAGGGAAAGACAACTCTGCTGGGATTGTGTCAAAGGTGGTGCTAGACACTGCCTTTCTCCGAGAGACAACAAATGGTGTTAGAATCAAGACATGGCAGGGAGGTTCGGGGTATGTTCGTGCCGTACGATTTGAAAACGTGAGAATGCAAGATGTTGAGAACCCAATTATCATAGATCAATTCTACTGTGACTCTCCTAATGCTTGTGAAACTCAGACATCTGCCATTAAAGTAAGCCAAATCATGTACCGGAACATAAGTGGAACCACAACCAGCAAGAACGCAATGAAATTCGCCTGTAGCGACAGTGTTCCATGCAGCAACATAATTCTAAGCAATGTCAATTTGGAGAAGACGGATGGCACAGTGGAGACCTATTGCCATGCTGCTCAAGGCTTCGGATATGGCGTCGTGCACCCATCGGCCGACTGCCTAACCTCCAGCGACAATGACTTTGCTGCTAAAGATCAAACCCAACTCCCTACTGAGCAGCACATTGTTCACACTGAACTCTGA

Protein sequence

MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSERGSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPCNDGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIVSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL
Homology
BLAST of Carg14476 vs. NCBI nr
Match: KAG7012807.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 928.7 bits (2399), Expect = 1.9e-266
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0

Query: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60
           MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER
Sbjct: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60

Query: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPCN 120
           GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPCN
Sbjct: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPCN 120

Query: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
           DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240
           KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300
           GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV
Sbjct: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300

Query: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 360
           SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE
Sbjct: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 360

Query: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
           TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420

Query: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 462
           QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 461

BLAST of Carg14476 vs. NCBI nr
Match: KAG6573734.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 926.4 bits (2393), Expect = 9.6e-266
Identity = 460/461 (99.78%), Postives = 460/461 (99.78%), Query Frame = 0

Query: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60
           MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER
Sbjct: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60

Query: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPCN 120
           GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEK YLVNATTFKGPCN
Sbjct: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKRYLVNATTFKGPCN 120

Query: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
           DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240
           KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300
           GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV
Sbjct: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300

Query: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 360
           SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE
Sbjct: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 360

Query: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
           TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420

Query: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 462
           QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 461

BLAST of Carg14476 vs. NCBI nr
Match: XP_022944797.1 (probable polygalacturonase At1g80170 [Cucurbita moschata])

HSP 1 Score: 923.7 bits (2386), Expect = 6.2e-265
Identity = 458/461 (99.35%), Postives = 459/461 (99.57%), Query Frame = 0

Query: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60
           MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER
Sbjct: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60

Query: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPCN 120
           GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEK YLVNATTFKGPCN
Sbjct: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKRYLVNATTFKGPCN 120

Query: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
           DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240
           KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300
           GIHITQS NVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV
Sbjct: 241 GIHITQSMNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300

Query: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 360
           SKVVLDTAFLRETTNGVRIKTWQGGSGYVRA+RFENVRMQDVENPIIIDQFYCDSPNACE
Sbjct: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAIRFENVRMQDVENPIIIDQFYCDSPNACE 360

Query: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
           TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420

Query: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 462
           QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 461

BLAST of Carg14476 vs. NCBI nr
Match: XP_023542310.1 (probable polygalacturonase At1g80170 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 922.5 bits (2383), Expect = 1.4e-264
Identity = 458/461 (99.35%), Postives = 459/461 (99.57%), Query Frame = 0

Query: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60
           MDKFFLLPLLSLLIIAYGVS SIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER
Sbjct: 1   MDKFFLLPLLSLLIIAYGVSCSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60

Query: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPCN 120
           GSRSLMNVDSFDAVGDG+TDDTMAFRKAWETACSAAKSVLLVPKEK YLVNATTFKGPCN
Sbjct: 61  GSRSLMNVDSFDAVGDGVTDDTMAFRKAWETACSAAKSVLLVPKEKRYLVNATTFKGPCN 120

Query: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
           DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240
           KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300
           GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV
Sbjct: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300

Query: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 360
           SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE
Sbjct: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 360

Query: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
           TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420

Query: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 462
           QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 461

BLAST of Carg14476 vs. NCBI nr
Match: XP_022966897.1 (probable polygalacturonase At1g80170 [Cucurbita maxima])

HSP 1 Score: 913.7 bits (2360), Expect = 6.5e-262
Identity = 456/464 (98.28%), Postives = 459/464 (98.92%), Query Frame = 0

Query: 1   MDKFF---LLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWK 60
           MDKFF   LLPLLSLLIIAYGVS SIDPESDIDMVKEL+TF+IEEQDDNVVDTYAIPVWK
Sbjct: 19  MDKFFLLPLLPLLSLLIIAYGVSCSIDPESDIDMVKELKTFNIEEQDDNVVDTYAIPVWK 78

Query: 61  SERGSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKG 120
           SERGSRSLMNVDSFDAVGDG+TDDTMAFRKAWETACSAAKSVLLVPKEK YLVNATTFKG
Sbjct: 79  SERGSRSLMNVDSFDAVGDGVTDDTMAFRKAWETACSAAKSVLLVPKEKRYLVNATTFKG 138

Query: 121 PCNDGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESC 180
           PCNDGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESC
Sbjct: 139 PCNDGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESC 198

Query: 181 KKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSP 240
           KKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSP
Sbjct: 199 KKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSP 258

Query: 241 NTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSA 300
           NTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSA
Sbjct: 259 NTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSA 318

Query: 301 GIVSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPN 360
           GIVSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPN
Sbjct: 319 GIVSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPN 378

Query: 361 ACETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYC 420
           ACETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYC
Sbjct: 379 ACETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYC 438

Query: 421 HAAQGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 462
           HAAQGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL
Sbjct: 439 HAAQGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 482

BLAST of Carg14476 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.2e-104
Identity = 189/384 (49.22%), Postives = 253/384 (65.89%), Query Frame = 0

Query: 62  SRSLMNVDSFDAVGDGITDDTMAFRKAWETACSA-AKSVLLVPKEKTYLVNATTFKGPCN 121
           S  L++V +F A G+G+TDDT AF  AW+TACS+  K+ +LVP+  T L+      GPC 
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCK 108

Query: 122 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 181
             + +QI GTIIAP++P+ W+    R+WL F  L+  T +G G ++G G++WW  SCK N
Sbjct: 109 ARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHN 168

Query: 182 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 241
            S PC+GAPTALT     N+RV+ L + +SQQMH  +     V I+ ++V +P  SPNTD
Sbjct: 169 HSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTD 228

Query: 242 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 301
           GIHI+ S  +V+ N  +STGDDCISIV  S+ I +  I CGPGHGISIGSLGK  S   V
Sbjct: 229 GIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEV 288

Query: 302 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 361
             + +DTA + +T NGVRIKTWQGGSG V  + F N++M +V NPIIIDQ+YCDS   C 
Sbjct: 289 RDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCA 348

Query: 362 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKT--DGTVETYCH 421
            QTSAI +  I + ++ GT+ SK A+K +CSDS PC NI+L +++LE +  DG  E++C 
Sbjct: 349 NQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCW 408

Query: 422 AAQGFGYGVVHPSADCLTSSDNDF 443
            A G   G V+P   CL S D  F
Sbjct: 409 EAYGSSSGQVYP-PPCL-SDDTSF 430

BLAST of Carg14476 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 1.3e-95
Identity = 179/366 (48.91%), Postives = 237/366 (64.75%), Query Frame = 0

Query: 66  MNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPCNDGMII 125
           +NVD F A GDG  DDT AF KAW+ ACS+  S +L+  +K YLV   +F GPC  G+ +
Sbjct: 90  VNVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSGLTM 149

Query: 126 QIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPC 185
           QI GTI A D+   +  K  R WL F  +     +G G I+G+G+ WW  SCK NK+ PC
Sbjct: 150 QIYGTIEASDDRSDY-RKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKALPC 209

Query: 186 KGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTDGIHIT 245
           K APTALT   S ++ VK L I+N+QQ+H +      V+ + + V++P +SPNTDGIH+T
Sbjct: 210 KDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTDGIHVT 269

Query: 246 QSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIVSKVVL 305
            + N+ + +C I TGDDCISIVN S  +++  I+CGPGHGISIGSLG  NS   VS VV+
Sbjct: 270 GTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHVSDVVV 329

Query: 306 DTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACETQTSA 365
           + A L  TTNGVRIKTWQGGSG    ++F+NV M +VENPIIIDQ YCD    C+ Q+SA
Sbjct: 330 NGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQEQSSA 389

Query: 366 IKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDG-TVETYCHAAQGFG 425
           ++V  ++Y+NI GT  S  A+ F CS   PC  I+L +V+LE   G   +  C+  +   
Sbjct: 390 VQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEIEGGAAAKALCNNVELSE 449

Query: 426 YGVVHP 431
            GVV P
Sbjct: 450 TGVVSP 453

BLAST of Carg14476 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 6.4e-95
Identity = 177/362 (48.90%), Postives = 234/362 (64.64%), Query Frame = 0

Query: 54  PVWKSERGSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNAT 113
           PV   E    + ++VD F A GDG TDDT AF KAW+ ACS+  SVL+VP+ K YL+   
Sbjct: 73  PVGGPETSPDTDISVDDFGARGDG-TDDTKAFEKAWKDACSSG-SVLIVPENKNYLLKQI 132

Query: 114 TFKGPCNDGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWW 173
           TF GPC   + ++I GTI A  +   W     ++W+EF  ++  T +G G  +G+GE WW
Sbjct: 133 TFSGPCKSDLRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWW 192

Query: 174 AESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSP 233
             SCK+ KS PCK APTALT  S  N+ V  L+I++SQ+MH +  + + V  + + V++P
Sbjct: 193 DSSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAP 252

Query: 234 GDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGK 293
             SPNTDGIHIT +  + V N  I TGDDCISI + S  +    I+CGPGHGISIGSLG 
Sbjct: 253 EHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGD 312

Query: 294 DNSAGIVSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYC 353
            NS   VS V++D   L +TTNG+RIKTWQGGSG  + ++F+N+ M +V NPIIIDQ+YC
Sbjct: 313 RNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYC 372

Query: 354 DSPNACETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTV 413
           DS + C  Q SA+KVS + Y NI GT+ S+ A+KF CS S PC   I+ N+NL    G  
Sbjct: 373 DSKDPCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGNGGKE 432

Query: 414 ET 416
            T
Sbjct: 433 TT 432

BLAST of Carg14476 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 8.6e-92
Identity = 171/375 (45.60%), Postives = 230/375 (61.33%), Query Frame = 0

Query: 65  LMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPCNDGMI 124
           + NV+ + AVGDG  D T AF K W  AC+   +V LVP  K ++VN   F GPC     
Sbjct: 59  VFNVEHYGAVGDGKHDSTDAFEKTWNAACNKLSAVFLVPANKKFVVNNLVFYGPCQPHFS 118

Query: 125 IQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRP 184
            +++GTI A  +P KW  K  + W+ F +L      G GVIDG G +WW++ CK    R 
Sbjct: 119 FKVDGTIAAYPDPAKW--KNSKIWMHFARLTDFNLMGTGVIDGQGNRWWSDQCKTINGRT 178

Query: 185 C---KGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTDG 244
               KG PTA+ ID S ++ VK LT+ NS + H      + V+I  +++ +P DSPNTDG
Sbjct: 179 VCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAPRDSPNTDG 238

Query: 245 IHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIVS 304
           I I  S    ++ C I TGDDC+++   SS I +K ++CGPGHG+SIGSLGK NS   VS
Sbjct: 239 IDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGKGNSRSEVS 298

Query: 305 KVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACET 364
            V LD A   +T NG+RIKTWQGGSG    + +ENV M + ENPI+I+QFYC S  AC+ 
Sbjct: 299 FVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFYCTSAAACKN 358

Query: 365 QTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHA-A 424
           Q SA+K+  + ++NI GT+ +  A++  CSDSVPCSNI LSNV L+ T G V T  +  A
Sbjct: 359 QRSAVKIQDVTFKNIHGTSATTAAIQLMCSDSVPCSNIKLSNVFLKLTSGKVATCVNKNA 418

Query: 425 QGFGYGVVHPSADCL 436
            G+    ++PS   L
Sbjct: 419 NGYYTNPLNPSCKSL 431

BLAST of Carg14476 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 337.4 bits (864), Expect = 2.5e-91
Identity = 169/349 (48.42%), Postives = 231/349 (66.19%), Query Frame = 0

Query: 60  RGSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLV-PKEKTYLVNATTFKGP 119
           R S    NV++F A  +G  DD+ AF KAWE ACS+   V +V PK + Y++ A TF GP
Sbjct: 65  RSSPRSFNVNTFGAKANG-NDDSKAFMKAWEAACSSTGIVYIVAPKNRDYMLKAVTFSGP 124

Query: 120 CNDGMII-QIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESC 179
           C   +II +I G I A + P   D K  R W+ F  +N    +G G IDG+G  WW +SC
Sbjct: 125 CKSSLIIFKIYGRIEAWENPS--DYKERRHWIVFENVNNLRVEGGGRIDGNGHIWWPKSC 184

Query: 180 KKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSP 239
           K N   PC GAPTA+T    +N+RV  + ++N+QQMH T    + V+   + V+SP DSP
Sbjct: 185 KINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSP 244

Query: 240 NTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSA 299
           NTDGIH++ + N+++Q+  + TGDDCISIV+ S  ++  GI+CGPGHGISIGSLG+DNS 
Sbjct: 245 NTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNSE 304

Query: 300 GIVSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPN 359
             VS VV++ A L  TTNGVRIKTWQGG G  + + F+++ M++V NPIII+Q YCD   
Sbjct: 305 AYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRVE 364

Query: 360 ACETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNL 407
           AC  Q SA++VS ++Y+NI GT++   A+KF CS ++PC  I + NV L
Sbjct: 365 ACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKL 410

BLAST of Carg14476 vs. ExPASy TrEMBL
Match: A0A6J1FZ30 (probable polygalacturonase At1g80170 OS=Cucurbita moschata OX=3662 GN=LOC111449225 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.0e-265
Identity = 458/461 (99.35%), Postives = 459/461 (99.57%), Query Frame = 0

Query: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60
           MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER
Sbjct: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60

Query: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPCN 120
           GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEK YLVNATTFKGPCN
Sbjct: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKRYLVNATTFKGPCN 120

Query: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
           DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240
           KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300
           GIHITQS NVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV
Sbjct: 241 GIHITQSMNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300

Query: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 360
           SKVVLDTAFLRETTNGVRIKTWQGGSGYVRA+RFENVRMQDVENPIIIDQFYCDSPNACE
Sbjct: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAIRFENVRMQDVENPIIIDQFYCDSPNACE 360

Query: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
           TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420

Query: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 462
           QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 461

BLAST of Carg14476 vs. ExPASy TrEMBL
Match: A0A6J1HP82 (probable polygalacturonase At1g80170 OS=Cucurbita maxima OX=3661 GN=LOC111466464 PE=3 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 3.1e-262
Identity = 456/464 (98.28%), Postives = 459/464 (98.92%), Query Frame = 0

Query: 1   MDKFF---LLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWK 60
           MDKFF   LLPLLSLLIIAYGVS SIDPESDIDMVKEL+TF+IEEQDDNVVDTYAIPVWK
Sbjct: 19  MDKFFLLPLLPLLSLLIIAYGVSCSIDPESDIDMVKELKTFNIEEQDDNVVDTYAIPVWK 78

Query: 61  SERGSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKG 120
           SERGSRSLMNVDSFDAVGDG+TDDTMAFRKAWETACSAAKSVLLVPKEK YLVNATTFKG
Sbjct: 79  SERGSRSLMNVDSFDAVGDGVTDDTMAFRKAWETACSAAKSVLLVPKEKRYLVNATTFKG 138

Query: 121 PCNDGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESC 180
           PCNDGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESC
Sbjct: 139 PCNDGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESC 198

Query: 181 KKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSP 240
           KKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSP
Sbjct: 199 KKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSP 258

Query: 241 NTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSA 300
           NTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSA
Sbjct: 259 NTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSA 318

Query: 301 GIVSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPN 360
           GIVSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPN
Sbjct: 319 GIVSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPN 378

Query: 361 ACETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYC 420
           ACETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYC
Sbjct: 379 ACETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYC 438

Query: 421 HAAQGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 462
           HAAQGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL
Sbjct: 439 HAAQGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 482

BLAST of Carg14476 vs. ExPASy TrEMBL
Match: A0A1S3BF58 (probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103489000 PE=3 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 3.3e-243
Identity = 417/469 (88.91%), Postives = 440/469 (93.82%), Query Frame = 0

Query: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60
           MDK FLL LL LLIIAYGVSG+   +SDIDM++ELE+FDIEE+DD+VV +Y +P W+SER
Sbjct: 38  MDKLFLLSLLGLLIIAYGVSGTFVSDSDIDMIEELESFDIEEEDDDVVYSYTVPAWRSER 97

Query: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPCN 120
           GS+ LMNVDSFDAVGDG+TDDTMAFRKAWETACS +KSVLLVPKEK YLVNATTFKGPC 
Sbjct: 98  GSKILMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKRYLVNATTFKGPCK 157

Query: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
           DGM+IQIEGTI+APDEPE WDPK PRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 158 DGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 217

Query: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240
           KSRPCKGAPTA TIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVS+PGDSPNTD
Sbjct: 218 KSRPCKGAPTAFTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSAPGDSPNTD 277

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300
           GIHITQSTNVVVQN KISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNS GIV
Sbjct: 278 GIHITQSTNVVVQNSKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 337

Query: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 360
           +KVVLDTAFLRETTNGVRIKTWQGGSGYVR+VRFENVRM+DVENPIIIDQFYCDSP  CE
Sbjct: 338 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE 397

Query: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
           TQTSA+K+SQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 398 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 457

Query: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQT--------QLPTEQHIVHTEL 462
           QGFGYG+VHPSADCLTSSDN+FAA DQT        QLPTEQHIVHTEL
Sbjct: 458 QGFGYGIVHPSADCLTSSDNNFAALDQTQVLELETVQLPTEQHIVHTEL 506

BLAST of Carg14476 vs. ExPASy TrEMBL
Match: A0A0A0KQ65 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600960 PE=3 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 7.2e-243
Identity = 416/469 (88.70%), Postives = 440/469 (93.82%), Query Frame = 0

Query: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60
           MDK FLL LL L IIAYGVSG+   +SDIDM+KELE+FDIEE+DD+ V +Y +PVW+SER
Sbjct: 1   MDKLFLLSLLGLFIIAYGVSGAFVSDSDIDMIKELESFDIEEEDDDDVYSYTVPVWRSER 60

Query: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPCN 120
           GS+ LMNVDSFDA+GDG+TDDTMAFRKAWETACS +KSVLLVPKEKTYLVNATTFKGPC 
Sbjct: 61  GSKILMNVDSFDAIGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKTYLVNATTFKGPCK 120

Query: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
           DGM+IQIEGTI+APDEPE WDPK PRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 121 DGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240
           KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRIT VRVS+PGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITGVRVSAPGDSPNTD 240

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300
           GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNS GIV
Sbjct: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 300

Query: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 360
           +KVVLDTA+LRETTNGVRIKTWQGGSGYVRAVRFENVRM+DVENPIIIDQFYCDSP  CE
Sbjct: 301 TKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCE 360

Query: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
           +QTSA+K+SQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 361 SQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420

Query: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQT--------QLPTEQHIVHTEL 462
           QGFGYGVVHPSADCLTSSDN+FA+ DQT        QL TEQHIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIVHTEL 469

BLAST of Carg14476 vs. ExPASy TrEMBL
Match: A0A6J1DBU9 (probable polygalacturonase At1g80170 OS=Momordica charantia OX=3673 GN=LOC111019073 PE=3 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 1.1e-235
Identity = 400/468 (85.47%), Postives = 435/468 (92.95%), Query Frame = 0

Query: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60
           MDKFF+L LL +LI+A GVSGSI+P+ DIDM++ELE FDI  +DD+VVD  A+PVW+SER
Sbjct: 1   MDKFFVLSLLGMLIVACGVSGSIEPDGDIDMIRELENFDIGGEDDDVVDASAVPVWRSER 60

Query: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPCN 120
           GS+ LMNVDSF+AVGDG++DDTMAFRKAWETACS +KSVLLVPK  TYLVNATTFKGPC 
Sbjct: 61  GSKVLMNVDSFNAVGDGVSDDTMAFRKAWETACSTSKSVLLVPKGGTYLVNATTFKGPCK 120

Query: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
           D ++IQIEGTI+APDEPEKWDPK  RQWLEFTKLNA TFQGDGVIDGSG+KWWAESCKKN
Sbjct: 121 DDLVIQIEGTIVAPDEPEKWDPKFQRQWLEFTKLNAVTFQGDGVIDGSGDKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240
           KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSE+VR+TEVRVS+PGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSESVRVTEVRVSAPGDSPNTD 240

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300
           GIHITQSTNVV+QNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNS GIV
Sbjct: 241 GIHITQSTNVVIQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 300

Query: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 360
           +KVVLDTAFLRETTNGVRIKTWQGGSGYVR+VRFENVRM+DVENPIIIDQFYCDSP +CE
Sbjct: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTSCE 360

Query: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
           TQTSA+K+SQIMYRNISGTTTSKNAMKFACSDSVPCSNI+LSNVNLEKTDGTVETYCH+A
Sbjct: 361 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIVLSNVNLEKTDGTVETYCHSA 420

Query: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQTQLP-------TEQHIVHTEL 462
           QGFGYGVVHPSADCLTSSD   AA DQT++P         +HIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLTSSDKYSAASDQTEVPELVELHLPTEHIVHTEL 468

BLAST of Carg14476 vs. TAIR 10
Match: AT1G23460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 621.3 bits (1601), Expect = 6.2e-178
Identity = 303/462 (65.58%), Postives = 363/462 (78.57%), Query Frame = 0

Query: 1   MDKFFLLPLLSLLII-AYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSE 60
           MDK F+L LL LL++ AYG +G +   +D+D++ ELE FD+   DD+       P + S 
Sbjct: 2   MDKLFILSLLGLLLVTAYGAAGKM-VYTDLDILDELENFDVLVDDDDDTKLLDWPSFTSR 61

Query: 61  RGSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGPC 120
              ++L+NVD+F A GDG++DDT AF  AW  ACS +KSV LVP+ + YLVNAT F GPC
Sbjct: 62  HSGKNLVNVDTFGAAGDGVSDDTQAFVSAWSKACSTSKSVFLVPEGRRYLVNATKFNGPC 121

Query: 121 NDGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKK 180
              +IIQI+GTI+APDEP  WD K  R WLEF+KL    FQG GVIDGSG KWWA SCKK
Sbjct: 122 EQKLIIQIDGTIVAPDEPSNWDSKFQRIWLEFSKLKGVVFQGKGVIDGSGSKWWAASCKK 181

Query: 181 NKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNT 240
           NKS PCK APTALTI+SSS ++V GLTIQNSQQM+F IARS++VR+++V VSSPGDSPNT
Sbjct: 182 NKSNPCKSAPTALTIESSSGVKVSGLTIQNSQQMNFIIARSDSVRVSKVMVSSPGDSPNT 241

Query: 241 DGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGI 300
           DGIHIT STNV++Q+CKI TGDDC+SIVNASS IKMK I CGPGHGISIGSLGKDN+ GI
Sbjct: 242 DGIHITGSTNVILQDCKIGTGDDCVSIVNASSNIKMKNIYCGPGHGISIGSLGKDNTTGI 301

Query: 301 VSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNAC 360
           V++VVLDTA LRETTNG+RIKT+QGGSGYV+ +RF NV MQDV NPI+IDQFYCDSP  C
Sbjct: 302 VTQVVLDTALLRETTNGLRIKTYQGGSGYVQGIRFTNVEMQDVANPILIDQFYCDSPTTC 361

Query: 361 ETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHA 420
           + QTSA+K+SQIMYRNI+GTT S  A+KFACSD+VPCS+I+L+NVNLE  DG VE YC++
Sbjct: 362 QNQTSAVKISQIMYRNITGTTKSAKAIKFACSDTVPCSHIVLNNVNLEGNDGQVEAYCNS 421

Query: 421 AQGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHTEL 462
           A+GFGYGV+HPSADCL S D+     DQT    E    H EL
Sbjct: 422 AEGFGYGVIHPSADCLYSHDD--KGLDQTHKSEEAETGHDEL 460

BLAST of Carg14476 vs. TAIR 10
Match: AT1G70500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 589.3 bits (1518), Expect = 2.6e-168
Identity = 291/461 (63.12%), Postives = 363/461 (78.74%), Query Frame = 0

Query: 1   MDKFFLLPLLSLLIIA-YGVSGSIDPESDIDMVKELETFDIEEQD-DNVVDTYAIPVWKS 60
           M K F++ L +LL++A YG +G +    ++D++ +LE  D+ E D ++ V  +    + S
Sbjct: 1   MIKPFIISLFALLMVASYGATGKM-LFKELDILGKLENLDVPEDDIEDDVTFFDFSSFTS 60

Query: 61  ERGSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKTYLVNATTFKGP 120
           +   ++L+NVDSF+A GDG++DDT AF +AW  ACSA  SVLLVP+ ++YLVNAT F GP
Sbjct: 61  QYSGKNLVNVDSFNASGDGVSDDTQAFIRAWTMACSAPNSVLLVPQGRSYLVNATKFDGP 120

Query: 121 CNDGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCK 180
           C + +IIQI+GTIIAPDEP +WDPK PR WL+F+KL    FQG+GVIDGSG KWWA SCK
Sbjct: 121 CQEKLIIQIDGTIIAPDEPSQWDPKFPRNWLQFSKLQGVVFQGNGVIDGSGTKWWAASCK 180

Query: 181 KNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPN 240
           KNKS PC GAPTALTI SSSN+ V+GLTI+NSQQMH  I RS TVRI+ V V+SPGDSPN
Sbjct: 181 KNKSNPCVGAPTALTIYSSSNVYVRGLTIRNSQQMHLIIQRSTTVRISRVMVTSPGDSPN 240

Query: 241 TDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAG 300
           TDGIHIT ST+VVVQ+ KISTGDDC+SIVN S+ IKMK I CGPGHGISIGSLG+ +S G
Sbjct: 241 TDGIHITASTDVVVQDSKISTGDDCVSIVNGSAKIKMKRIYCGPGHGISIGSLGQGHSKG 300

Query: 301 IVSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNA 360
            V+ VVL+TAFL+ TTNG+RIKTWQGG+GYV+ VRFENV MQDV NPIIIDQFYCDSP+ 
Sbjct: 301 TVTAVVLETAFLKNTTNGLRIKTWQGGNGYVKGVRFENVVMQDVANPIIIDQFYCDSPST 360

Query: 361 CETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCH 420
           C+ QTSA+ +S+IMYRNI+GTT S  A+ F CSD+VPCS+I+L+N+NLE  DG VE YC+
Sbjct: 361 CQNQTSAVHISEIMYRNITGTTKSSKAINFKCSDAVPCSHIVLNNINLEGNDGKVEAYCN 420

Query: 421 AAQGFGYGVVHPSADCLTSSDNDFAAKDQTQLPTEQHIVHT 460
           +A+GFGYGVVHPSADCL S D      D++   T+Q++  T
Sbjct: 421 SAEGFGYGVVHPSADCLYSHD------DKSLNQTDQYLAET 454

BLAST of Carg14476 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 381.7 bits (979), Expect = 8.2e-106
Identity = 189/384 (49.22%), Postives = 253/384 (65.89%), Query Frame = 0

Query: 62  SRSLMNVDSFDAVGDGITDDTMAFRKAWETACSA-AKSVLLVPKEKTYLVNATTFKGPCN 121
           S  L++V +F A G+G+TDDT AF  AW+TACS+  K+ +LVP+  T L+      GPC 
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCK 108

Query: 122 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 181
             + +QI GTIIAP++P+ W+    R+WL F  L+  T +G G ++G G++WW  SCK N
Sbjct: 109 ARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHN 168

Query: 182 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 241
            S PC+GAPTALT     N+RV+ L + +SQQMH  +     V I+ ++V +P  SPNTD
Sbjct: 169 HSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTD 228

Query: 242 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 301
           GIHI+ S  +V+ N  +STGDDCISIV  S+ I +  I CGPGHGISIGSLGK  S   V
Sbjct: 229 GIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEV 288

Query: 302 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 361
             + +DTA + +T NGVRIKTWQGGSG V  + F N++M +V NPIIIDQ+YCDS   C 
Sbjct: 289 RDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCA 348

Query: 362 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKT--DGTVETYCH 421
            QTSAI +  I + ++ GT+ SK A+K +CSDS PC NI+L +++LE +  DG  E++C 
Sbjct: 349 NQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCW 408

Query: 422 AAQGFGYGVVHPSADCLTSSDNDF 443
            A G   G V+P   CL S D  F
Sbjct: 409 EAYGSSSGQVYP-PPCL-SDDTSF 430

BLAST of Carg14476 vs. TAIR 10
Match: AT3G07970.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 337.4 bits (864), Expect = 1.8e-92
Identity = 169/349 (48.42%), Postives = 231/349 (66.19%), Query Frame = 0

Query: 60  RGSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLV-PKEKTYLVNATTFKGP 119
           R S    NV++F A  +G  DD+ AF KAWE ACS+   V +V PK + Y++ A TF GP
Sbjct: 65  RSSPRSFNVNTFGAKANG-NDDSKAFMKAWEAACSSTGIVYIVAPKNRDYMLKAVTFSGP 124

Query: 120 CNDGMII-QIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESC 179
           C   +II +I G I A + P   D K  R W+ F  +N    +G G IDG+G  WW +SC
Sbjct: 125 CKSSLIIFKIYGRIEAWENPS--DYKERRHWIVFENVNNLRVEGGGRIDGNGHIWWPKSC 184

Query: 180 KKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSP 239
           K N   PC GAPTA+T    +N+RV  + ++N+QQMH T    + V+   + V+SP DSP
Sbjct: 185 KINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSP 244

Query: 240 NTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSA 299
           NTDGIH++ + N+++Q+  + TGDDCISIV+ S  ++  GI+CGPGHGISIGSLG+DNS 
Sbjct: 245 NTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNSE 304

Query: 300 GIVSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPN 359
             VS VV++ A L  TTNGVRIKTWQGG G  + + F+++ M++V NPIII+Q YCD   
Sbjct: 305 AYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRVE 364

Query: 360 ACETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNL 407
           AC  Q SA++VS ++Y+NI GT++   A+KF CS ++PC  I + NV L
Sbjct: 365 ACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKL 410

BLAST of Carg14476 vs. TAIR 10
Match: AT3G57510.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 336.7 bits (862), Expect = 3.0e-92
Identity = 171/368 (46.47%), Postives = 239/368 (64.95%), Query Frame = 0

Query: 64  SLMNVDSFDAVGDGITDDTMAFRKAWETACSA-AKSVLLVPKEKTYLVNATTFKGPCNDG 123
           S ++V +F A GDG TDDT AF+KAW+ ACS    +  LVPK KTYL+ +T F+GPC   
Sbjct: 66  STVSVSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKTYLLKSTRFRGPCKSL 125

Query: 124 MIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQG--DGVIDGSGEKWWAESCKKN 183
              QI GT+ A    ++ D K    WL    +N  +  G   G+I+G+G+ WW  SCK +
Sbjct: 126 RNFQILGTLSA--STKRSDYKDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCKID 185

Query: 184 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 243
           KS+PC  APTALT+ +  N+ VK L ++N+QQ+  +I +   V ++ V +++PGDSPNTD
Sbjct: 186 KSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTD 245

Query: 244 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 303
           GIHIT + N+ V N  I TGDDCISI + +  +++  ++CGPGHGISIGSLG DNS   V
Sbjct: 246 GIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKAYV 305

Query: 304 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 363
           S + +D A   E+ NGVRIKT+QGGSG  + ++F+N+RM++V+NPIIIDQ YCD  + CE
Sbjct: 306 SGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPIIIDQDYCDK-DKCE 365

Query: 364 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLE-----------KT 418
            Q SA++V  ++Y+NISGT+ +  A+   CS+  PC  I+L NV ++           K 
Sbjct: 366 DQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENVKIKGGTASCKNANVKN 425

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7012807.11.9e-266100.00putative polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6573734.19.6e-26699.78putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022944797.16.2e-26599.35probable polygalacturonase At1g80170 [Cucurbita moschata][more]
XP_023542310.11.4e-26499.35probable polygalacturonase At1g80170 [Cucurbita pepo subsp. pepo][more]
XP_022966897.16.5e-26298.28probable polygalacturonase At1g80170 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q94AJ51.2e-10449.22Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P353361.3e-9548.91Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Q020966.4e-9548.90Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1[more]
Q9FY198.6e-9245.60Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
Q9SFB72.5e-9148.42Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1FZ303.0e-26599.35probable polygalacturonase At1g80170 OS=Cucurbita moschata OX=3662 GN=LOC1114492... [more]
A0A6J1HP823.1e-26298.28probable polygalacturonase At1g80170 OS=Cucurbita maxima OX=3661 GN=LOC111466464... [more]
A0A1S3BF583.3e-24388.91probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103489000 PE=... [more]
A0A0A0KQ657.2e-24388.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600960 PE=3 SV=1[more]
A0A6J1DBU91.1e-23585.47probable polygalacturonase At1g80170 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
Match NameE-valueIdentityDescription
AT1G23460.16.2e-17865.58Pectin lyase-like superfamily protein [more]
AT1G70500.12.6e-16863.12Pectin lyase-like superfamily protein [more]
AT1G80170.18.2e-10649.22Pectin lyase-like superfamily protein [more]
AT3G07970.11.8e-9248.42Pectin lyase-like superfamily protein [more]
AT3G57510.13.0e-9246.47Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 270..290
e-value: 8900.0
score: 0.5
coord: 220..246
e-value: 71.0
score: 12.3
coord: 396..417
e-value: 7500.0
score: 1.0
coord: 247..268
e-value: 18.0
score: 14.2
coord: 329..350
e-value: 5500.0
score: 2.0
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 96..417
e-value: 6.4E-93
score: 311.4
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 277..290
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 55..460
e-value: 6.2E-118
score: 396.3
NoneNo IPR availablePANTHERPTHR31375:SF205BNAA08G20470D PROTEINcoord: 2..445
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 2..445
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 63..436

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14476-RACarg14476-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008234 cysteine-type peptidase activity
molecular_function GO:0004650 polygalacturonase activity