Carg14462 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14462
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionXyloglucan endotransglucosylase/hydrolase
LocationCarg_Chr18: 8468244 .. 8469457 (+)
RNA-Seq ExpressionCarg14462
SyntenyCarg14462
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATTTCCAGAGGAGTAATGGAATTGCGCCTTTTGAGCTTCATCTTTTTGTGTATGTTGTTTGTTGTCCCTTCACGTGGTAGATTTACTAGATATTACACGCCTCCAAACGTCCCACGTTTGACAGATCTCGTTCCTCGTGTCTCGATTGATCAATGCTTTGCTAAATTGTTTGGGGCCTCAAATATGCGGCTGATGAACAATGGGTCCTCTGTCAATCTCACTCTCGACAAAGTTTCTGGTAAATCACCTTTGGATCTTAACTTTTCCTTTAGTCTGACTTTCACTTTCATTTCTAACTTTTTCTCTCTTCATCTTTGGAGGTGCTGGTCTGGTCTCCAGAAACAAATATCATTATGGATTCTTCAGTGCTTCAATTAAGCTTCCATCAGGTCTCACTTCAGGGGTTGTGGTGGCTTTTTATGTAAGTTCTTTTTTTTCTCTCTACTGTTCTCAGTTTGGCTTACCAGTTTGATGCATAGCTAACGAGATGAACACCGCGTTCAGTTGTCGAATGCAGATGTTTATCCCCATTCTCACGACGAGATAGACATCGAGCTGCTTGGACATGACAAGAGAAAAGATTGGGTTATTCAAACAAATATCTATGCCAATGGAAGTGTCAAGACTGGAAGAGAAGAGAAATTTTACCTGTGGTTTGATCCCAGCTTGAAGTACCATGATTACACCATCATTTGGAACAATTATCATACAGTGTAAGTGCATGAAATTCAGTCTCAAAAATAAACCCAAAAATGGGTCTTTGCTTTTCTTATTGGATCAATCACAATGTTTCTTAGAAAGTGCCTCGAAATGCAAGCAGGTTTCTGGTGGATAATATTCCAGTAAGAGAGCTAAAAAATTCTGAAGCTTTCTACCCATCAAAGCCAATGTCAGTTTTTGTCACAATATGGGATGGTTCAGAATGGGCAACTCATGGAGGCAAATACCCAGTTGACTACAAGTATGCACCCTATGTAGCTTCTTTTGAAGAAATGGAAATCAATGGCAGTATGACAACTTCGACACCCACTTCTCCTTCAGGTTCTAAAACCAATGTCTCCAGCCCAGATACTCTGGAGCGGCCAGAATTTATCAAGCTATCACAGCAGCAAATAGAGGCAATGGATTGGGCAAGGAGGAAGCTCATGTTCTACTCCTATTGTAAAGATACATCTAGATACAAAGTTCTACCACCAGAGTGCAAATAA

mRNA sequence

ATGGAGATTTCCAGAGGAGTAATGGAATTGCGCCTTTTGAGCTTCATCTTTTTGTGTATGTTGTTTGTTGTCCCTTCACGTGGTAGATTTACTAGATATTACACGCCTCCAAACGTCCCACGTTTGACAGATCTCGTTCCTCGTGTCTCGATTGATCAATGCTTTGCTAAATTGTTTGGGGCCTCAAATATGCGGCTGATGAACAATGGGTCCTCTGTCAATCTCACTCTCGACAAAGTTTCTGGTGCTGGTCTGGTCTCCAGAAACAAATATCATTATGGATTCTTCAGTGCTTCAATTAAGCTTCCATCAGGTCTCACTTCAGGGGTTGTGGTGGCTTTTTATTTGTCGAATGCAGATGTTTATCCCCATTCTCACGACGAGATAGACATCGAGCTGCTTGGACATGACAAGAGAAAAGATTGGGTTATTCAAACAAATATCTATGCCAATGGAAGTGTCAAGACTGGAAGAGAAGAGAAATTTTACCTGTGGTTTGATCCCAGCTTGAAGTACCATGATTACACCATCATTTGGAACAATTATCATACAGTGTTTCTGGTGGATAATATTCCAGTAAGAGAGCTAAAAAATTCTGAAGCTTTCTACCCATCAAAGCCAATGTCAGTTTTTGTCACAATATGGGATGGTTCAGAATGGGCAACTCATGGAGGCAAATACCCAGTTGACTACAAGTATGCACCCTATGTAGCTTCTTTTGAAGAAATGGAAATCAATGGCAGTATGACAACTTCGACACCCACTTCTCCTTCAGGTTCTAAAACCAATGTCTCCAGCCCAGATACTCTGGAGCGGCCAGAATTTATCAAGCTATCACAGCAGCAAATAGAGGCAATGGATTGGGCAAGGAGGAAGCTCATGTTCTACTCCTATTGTAAAGATACATCTAGATACAAAGTTCTACCACCAGAGTGCAAATAA

Coding sequence (CDS)

ATGGAGATTTCCAGAGGAGTAATGGAATTGCGCCTTTTGAGCTTCATCTTTTTGTGTATGTTGTTTGTTGTCCCTTCACGTGGTAGATTTACTAGATATTACACGCCTCCAAACGTCCCACGTTTGACAGATCTCGTTCCTCGTGTCTCGATTGATCAATGCTTTGCTAAATTGTTTGGGGCCTCAAATATGCGGCTGATGAACAATGGGTCCTCTGTCAATCTCACTCTCGACAAAGTTTCTGGTGCTGGTCTGGTCTCCAGAAACAAATATCATTATGGATTCTTCAGTGCTTCAATTAAGCTTCCATCAGGTCTCACTTCAGGGGTTGTGGTGGCTTTTTATTTGTCGAATGCAGATGTTTATCCCCATTCTCACGACGAGATAGACATCGAGCTGCTTGGACATGACAAGAGAAAAGATTGGGTTATTCAAACAAATATCTATGCCAATGGAAGTGTCAAGACTGGAAGAGAAGAGAAATTTTACCTGTGGTTTGATCCCAGCTTGAAGTACCATGATTACACCATCATTTGGAACAATTATCATACAGTGTTTCTGGTGGATAATATTCCAGTAAGAGAGCTAAAAAATTCTGAAGCTTTCTACCCATCAAAGCCAATGTCAGTTTTTGTCACAATATGGGATGGTTCAGAATGGGCAACTCATGGAGGCAAATACCCAGTTGACTACAAGTATGCACCCTATGTAGCTTCTTTTGAAGAAATGGAAATCAATGGCAGTATGACAACTTCGACACCCACTTCTCCTTCAGGTTCTAAAACCAATGTCTCCAGCCCAGATACTCTGGAGCGGCCAGAATTTATCAAGCTATCACAGCAGCAAATAGAGGCAATGGATTGGGCAAGGAGGAAGCTCATGTTCTACTCCTATTGTAAAGATACATCTAGATACAAAGTTCTACCACCAGAGTGCAAATAA

Protein sequence

MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFGASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWNNYHTVFLVDNIPVRELKNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASFEEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCKDTSRYKVLPPECK
Homology
BLAST of Carg14462 vs. NCBI nr
Match: KAG7012822.1 (putative xyloglucan endotransglucosylase/hydrolase protein 33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 647.5 bits (1669), Expect = 5.9e-182
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG 60
           MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG
Sbjct: 1   MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG 60

Query: 61  ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120
           ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD
Sbjct: 61  ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120

Query: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180
           VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN
Sbjct: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180

Query: 181 NYHTVFLVDNIPVRELKNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240
           NYHTVFLVDNIPVRELKNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF
Sbjct: 181 NYHTVFLVDNIPVRELKNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240

Query: 241 EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300
           EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK
Sbjct: 241 EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300

Query: 301 DTSRYKVLPPECK 314
           DTSRYKVLPPECK
Sbjct: 301 DTSRYKVLPPECK 313

BLAST of Carg14462 vs. NCBI nr
Match: XP_022945421.1 (probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucurbita moschata] >XP_023541843.1 probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 646.4 bits (1666), Expect = 1.3e-181
Identity = 312/313 (99.68%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG 60
           MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG
Sbjct: 1   MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG 60

Query: 61  ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120
           ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD
Sbjct: 61  ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120

Query: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180
           VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN
Sbjct: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180

Query: 181 NYHTVFLVDNIPVRELKNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240
           NYHTVFLVDNIPVREL+NSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF
Sbjct: 181 NYHTVFLVDNIPVRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240

Query: 241 EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300
           EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK
Sbjct: 241 EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300

Query: 301 DTSRYKVLPPECK 314
           DTSRYKVLPPECK
Sbjct: 301 DTSRYKVLPPECK 313

BLAST of Carg14462 vs. NCBI nr
Match: XP_022966887.1 (probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucurbita maxima])

HSP 1 Score: 630.6 bits (1625), Expect = 7.4e-177
Identity = 304/313 (97.12%), Postives = 308/313 (98.40%), Query Frame = 0

Query: 1   MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG 60
           MEISR VMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRV IDQCFAKLFG
Sbjct: 1   MEISRDVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVLIDQCFAKLFG 60

Query: 61  ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120
           ASNM+LMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD
Sbjct: 61  ASNMQLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120

Query: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180
           VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN
Sbjct: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180

Query: 181 NYHTVFLVDNIPVRELKNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240
           NYHTVFLVDNIPVREL+NS AFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF
Sbjct: 181 NYHTVFLVDNIPVRELRNSAAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240

Query: 241 EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300
           EEMEINGSMTT T T+PSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK
Sbjct: 241 EEMEINGSMTTPTATAPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300

Query: 301 DTSRYKVLPPECK 314
           DTSRYKV+PPECK
Sbjct: 301 DTSRYKVIPPECK 313

BLAST of Carg14462 vs. NCBI nr
Match: XP_038892286.1 (probable xyloglucan endotransglucosylase/hydrolase protein 33 isoform X2 [Benincasa hispida])

HSP 1 Score: 585.9 bits (1509), Expect = 2.1e-163
Identity = 282/313 (90.10%), Postives = 298/313 (95.21%), Query Frame = 0

Query: 1   MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG 60
           M  SRG+MEL LLSFIFLC+LF VPS     RYYTPP+VPRLTDLVP VSIDQCFAK+FG
Sbjct: 1   MGFSRGIMELPLLSFIFLCILFAVPS---CARYYTPPSVPRLTDLVPHVSIDQCFAKIFG 60

Query: 61  ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120
           ASN++L NNGSSV+LTLDKVSGAGLVSRNKYHYGFFSASIKLP+GLTSGVVVAFYLSNAD
Sbjct: 61  ASNIQLRNNGSSVDLTLDKVSGAGLVSRNKYHYGFFSASIKLPAGLTSGVVVAFYLSNAD 120

Query: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180
           VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPS+KYHDYTIIWN
Sbjct: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSVKYHDYTIIWN 180

Query: 181 NYHTVFLVDNIPVRELKNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240
           NYHTVFLVDNIPVREL+NSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYK+APYVASF
Sbjct: 181 NYHTVFLVDNIPVRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYVASF 240

Query: 241 EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300
           EEMEINGS++T T T PSGSKTNVSSPDT+E PEFIKLSQQQI+AMDWARRKLMFYSYCK
Sbjct: 241 EEMEINGSISTPTATVPSGSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCK 300

Query: 301 DTSRYKVLPPECK 314
           DTSRY+VLPPECK
Sbjct: 301 DTSRYRVLPPECK 310

BLAST of Carg14462 vs. NCBI nr
Match: XP_004135238.1 (probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucumis sativus] >KGN51813.1 hypothetical protein Csa_008560 [Cucumis sativus])

HSP 1 Score: 576.2 bits (1484), Expect = 1.7e-160
Identity = 277/313 (88.50%), Postives = 290/313 (92.65%), Query Frame = 0

Query: 1   MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG 60
           M  SRG+MEL LLSFIFLCMLFV PS    +RYYTPP+VPRLTDLVPRVS DQCFAK+FG
Sbjct: 1   MGFSRGIMELPLLSFIFLCMLFVAPS---CSRYYTPPSVPRLTDLVPRVSTDQCFAKIFG 60

Query: 61  ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120
           ASN++L NNGSSV+LTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD
Sbjct: 61  ASNIQLRNNGSSVDLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120

Query: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180
           VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN
Sbjct: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180

Query: 181 NYHTVFLVDNIPVRELKNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240
           NYHTVFLVDN+PVREL+NSE FYPSKPMSVFVTIWDGSEWATHGGKYPVDYK+APY ASF
Sbjct: 181 NYHTVFLVDNVPVRELRNSEVFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYTASF 240

Query: 241 EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300
           EEMEING + T   T PS SK NVS PDT E PEFIKLSQQQ++AMDWARRKLMFYSYCK
Sbjct: 241 EEMEINGGILTPKATVPSSSKANVSGPDTAEGPEFIKLSQQQVDAMDWARRKLMFYSYCK 300

Query: 301 DTSRYKVLPPECK 314
           DTSRYKVLPPECK
Sbjct: 301 DTSRYKVLPPECK 310

BLAST of Carg14462 vs. ExPASy Swiss-Prot
Match: Q8LC45 (Probable xyloglucan endotransglucosylase/hydrolase protein 33 OS=Arabidopsis thaliana OX=3702 GN=XTH33 PE=1 SV=2)

HSP 1 Score: 387.5 bits (994), Expect = 1.4e-106
Identity = 190/304 (62.50%), Postives = 231/304 (75.99%), Query Frame = 0

Query: 18  LCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFGASNMRLMNNGSSVNLTL 77
           LC L +V S    +R +T PNV RLTD   +++I+  F++ FGA N+++  NGS   LTL
Sbjct: 13  LCSLSLVSSH---SRKFTTPNVTRLTDQFSKIAIENGFSRRFGAHNIQV--NGSLAKLTL 72

Query: 78  DKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHD 137
           DK SGAGLVS+NKYHYGFFSA +KLP+G  SGVVVAFYLSNA+ YP SHDEIDIELLG  
Sbjct: 73  DKSSGAGLVSKNKYHYGFFSARLKLPAGFASGVVVAFYLSNAETYPKSHDEIDIELLGRS 132

Query: 138 KRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWNNYHTVFLVDNIPVRELK 197
           +R DW IQTN+YANGS +TGREEKFY WFDP+  +HDYT+IWN++HTVFLVDNIPVR+  
Sbjct: 133 RRDDWTIQTNVYANGSTRTGREEKFYFWFDPTQAFHDYTLIWNSHHTVFLVDNIPVRQFP 192

Query: 198 NSEAF---YPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASFEEMEI------NGS 257
           N  AF   YPSKPMS++VT+WDGSEWAT GGKYPV+YKYAP+V S  ++E+      NGS
Sbjct: 193 NRGAFTSAYPSKPMSLYVTVWDGSEWATKGGKYPVNYKYAPFVVSVADVELSGCSVNNGS 252

Query: 258 MTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCKDTSRYKVL 313
            T S P + SG   ++SS D ++  +F  LS+ QI AMDWARRKLMFYSYC D  RYKV+
Sbjct: 253 STGSGPCTKSGG--SISSLDPVDGQDFATLSKNQINAMDWARRKLMFYSYCSDKPRYKVM 309

BLAST of Carg14462 vs. ExPASy Swiss-Prot
Match: Q8LDS2 (Probable xyloglucan endotransglucosylase/hydrolase protein 27 OS=Arabidopsis thaliana OX=3702 GN=XTH27 PE=2 SV=2)

HSP 1 Score: 273.5 bits (698), Expect = 3.0e-72
Identity = 130/270 (48.15%), Postives = 185/270 (68.52%), Query Frame = 0

Query: 46  VPRVSIDQCFAKLFGASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSG 105
           +P  S ++ + +LFG  N+ +  +G SV LTLD+ +G+G VS + Y +GFFSASIKLPS 
Sbjct: 26  LPITSFEESYTQLFGDKNLFVHQDGKSVRLTLDERTGSGFVSNDYYLHGFFSASIKLPSD 85

Query: 106 LTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLW 165
            T+GVVVAFY+SN D+Y  +HDEID E LG+ + K+W +QTNIY NGS  +GREE++ LW
Sbjct: 86  YTAGVVVAFYMSNGDMYEKNHDEIDFEFLGNIREKEWRVQTNIYGNGSTHSGREERYNLW 145

Query: 166 FDPSLKYHDYTIIWNNYHTVFLVDNIPVRELKNSE---AFYPSKPMSVFVTIWDGSEWAT 225
           FDP+  +H Y+I+W++ H +F VDN+P+RE+K +      +PSKPMS++ TIWDGS+WAT
Sbjct: 146 FDPTEDFHQYSILWSDSHIIFFVDNVPIREVKRTAEMGGHFPSKPMSLYTTIWDGSKWAT 205

Query: 226 HGGKYPVDYKYAPYVASFEEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQ 285
           +GGKY V+YKYAPY+A F ++ ++G         P   + +  + + +   + I  SQ+ 
Sbjct: 206 NGGKYGVNYKYAPYIARFSDLVLHGCPVDPIEQFP---RCDEGAAEDMRAAQEITPSQR- 265

Query: 286 IEAMDWARRKLMFYSYCKDTSRYKVLPPEC 313
              MD  RR+LM YSYC D +RY V   EC
Sbjct: 266 -SKMDVFRRRLMTYSYCYDRARYNVALSEC 290

BLAST of Carg14462 vs. ExPASy Swiss-Prot
Match: Q38909 (Probable xyloglucan endotransglucosylase/hydrolase protein 28 OS=Arabidopsis thaliana OX=3702 GN=XTH28 PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 2.4e-69
Identity = 126/270 (46.67%), Postives = 182/270 (67.41%), Query Frame = 0

Query: 46  VPRVSIDQCFAKLFGASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSG 105
           +P +  D+ + +LFG  N+ +  +G SV LTLD+ +G+G VS + Y +GFFS+SIKLP+ 
Sbjct: 26  LPLIQFDEGYTQLFGDQNLIVHRDGKSVRLTLDERTGSGFVSNDIYLHGFFSSSIKLPAD 85

Query: 106 LTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLW 165
            ++GVV+AFYLSN D+Y  +HDEID E LG+ + ++W IQTNIY NGS   GREE++ LW
Sbjct: 86  YSAGVVIAFYLSNGDLYEKNHDEIDFEFLGNIRGREWRIQTNIYGNGSTHLGREERYNLW 145

Query: 166 FDPSLKYHDYTIIWNNYHTVFLVDNIPVRELKNSEAF---YPSKPMSVFVTIWDGSEWAT 225
           FDP+  +H Y+I+W+  H +F VDN+P+RE+K + +    +P+KPMS++ TIWDGS+WAT
Sbjct: 146 FDPTEDFHQYSILWSLSHIIFYVDNVPIREVKRTASMGGDFPAKPMSLYSTIWDGSKWAT 205

Query: 226 HGGKYPVDYKYAPYVASFEEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQ 285
            GGKY V+YKYAPYV+ F ++ ++G     T   PS     V +          ++++ Q
Sbjct: 206 DGGKYGVNYKYAPYVSQFTDLILHGCAVDPTEKFPSCKDEAVQNLRLAS-----EITESQ 265

Query: 286 IEAMDWARRKLMFYSYCKDTSRYKVLPPEC 313
              M+  R+K M YSYC D  RYKV+  EC
Sbjct: 266 RNKMEIFRQKHMTYSYCYDHMRYKVVLSEC 290

BLAST of Carg14462 vs. ExPASy Swiss-Prot
Match: Q38908 (Probable xyloglucan endotransglucosylase/hydrolase protein 30 OS=Arabidopsis thaliana OX=3702 GN=XTH30 PE=2 SV=2)

HSP 1 Score: 253.1 bits (645), Expect = 4.2e-66
Identity = 119/267 (44.57%), Postives = 173/267 (64.79%), Query Frame = 0

Query: 49  VSIDQCFAKLFGASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTS 108
           +S ++  + LFG +N+    +  SV L LD+ +G+G +S N Y +GF+S+ IKLP+  T+
Sbjct: 30  LSFEESLSPLFGDANLVRSPDDLSVRLLLDRYTGSGFISSNMYQHGFYSSMIKLPADYTA 89

Query: 109 GVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDP 168
           GVVVAFY SN DV+  +HDE+DIE LG+ K K W  QTN+Y NGS   GREE++ LWFDP
Sbjct: 90  GVVVAFYTSNGDVFEKTHDELDIEFLGNIKGKPWRFQTNLYGNGSTHRGREERYRLWFDP 149

Query: 169 SLKYHDYTIIWNNYHTVFLVDNIPVRELKNSEAF---YPSKPMSVFVTIWDGSEWATHGG 228
           S ++H Y+I+W  +  +F VD++P+RE+  ++A    YP+KPM+++ TIWD S+WAT GG
Sbjct: 150 SKEFHRYSILWTPHKIIFWVDDVPIREVIRNDAMGADYPAKPMALYATIWDASDWATSGG 209

Query: 229 KYPVDYKYAPYVASFEEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEA 288
           KY  +YK+AP+VA F+   ++G         P     +V   D LE  ++  ++  Q  A
Sbjct: 210 KYKANYKFAPFVAEFKSFSLDGCSVDPIQEVPMDCSDSV---DFLESQDYSSINSHQRAA 269

Query: 289 MDWARRKLMFYSYCKDTSRYKVLPPEC 313
           M   R++ M+YSYC DT RY    PEC
Sbjct: 270 MRRFRQRFMYYSYCYDTLRYPEPLPEC 293

BLAST of Carg14462 vs. ExPASy Swiss-Prot
Match: Q8L7H3 (Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis thaliana OX=3702 GN=XTH29 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.0e-64
Identity = 121/275 (44.00%), Postives = 175/275 (63.64%), Query Frame = 0

Query: 49  VSIDQCFAKLFGASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTS 108
           +  D+  + LFG  N+    +  SV L LDK +G+G +S + Y +GFFS+ IKLP   T+
Sbjct: 38  IFFDEGLSHLFGEGNLIRSPDDRSVRLLLDKYTGSGFISSSMYQHGFFSSLIKLPGAYTA 97

Query: 109 GVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDP 168
           G+VVAFY SN DV+   HDE+DIE LG+ + K W  QTN+Y NGS   GREE++ LWFDP
Sbjct: 98  GIVVAFYTSNGDVFVKDHDELDIEFLGNLEGKPWRFQTNMYGNGSTNRGREERYRLWFDP 157

Query: 169 SLKYHDYTIIWNNYHTVFLVDNIPVRELKNSEAF---YPSKPMSVFVTIWDGSEWATHGG 228
           S ++H Y+I+W     +F VD++P+RE+   E     YP KPMS++ TIWD S WAT GG
Sbjct: 158 SKEFHRYSILWTPTKIIFWVDDVPIREILRKEEMNGDYPQKPMSLYATIWDASSWATSGG 217

Query: 229 KYPVDYKYAPYVASFEEMEING-SMTTSTP----TSPSGSKTNVS---SPDTLERPEFIK 288
           K+ VDY ++P+V+ F+++ ++G +++ S P     +  G+  N++   S   L   ++  
Sbjct: 218 KFGVDYTFSPFVSEFKDIALDGCNVSDSFPGENNNNNIGNYNNINCSVSDQFLMSNDYST 277

Query: 289 LSQQQIEAMDWARRKLMFYSYCKDTSRYKVLPPEC 313
           +S +Q  AM   R + M+YSYC DT RY V PPEC
Sbjct: 278 ISPKQATAMRRFRERYMYYSYCYDTIRYSVPPPEC 312

BLAST of Carg14462 vs. ExPASy TrEMBL
Match: A0A6J1G0T5 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111449658 PE=3 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 6.3e-182
Identity = 312/313 (99.68%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG 60
           MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG
Sbjct: 1   MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG 60

Query: 61  ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120
           ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD
Sbjct: 61  ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120

Query: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180
           VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN
Sbjct: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180

Query: 181 NYHTVFLVDNIPVRELKNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240
           NYHTVFLVDNIPVREL+NSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF
Sbjct: 181 NYHTVFLVDNIPVRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240

Query: 241 EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300
           EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK
Sbjct: 241 EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300

Query: 301 DTSRYKVLPPECK 314
           DTSRYKVLPPECK
Sbjct: 301 DTSRYKVLPPECK 313

BLAST of Carg14462 vs. ExPASy TrEMBL
Match: A0A6J1HP76 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111466454 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 3.6e-177
Identity = 304/313 (97.12%), Postives = 308/313 (98.40%), Query Frame = 0

Query: 1   MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG 60
           MEISR VMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRV IDQCFAKLFG
Sbjct: 1   MEISRDVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVLIDQCFAKLFG 60

Query: 61  ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120
           ASNM+LMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD
Sbjct: 61  ASNMQLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120

Query: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180
           VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN
Sbjct: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180

Query: 181 NYHTVFLVDNIPVRELKNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240
           NYHTVFLVDNIPVREL+NS AFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF
Sbjct: 181 NYHTVFLVDNIPVRELRNSAAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240

Query: 241 EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300
           EEMEINGSMTT T T+PSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK
Sbjct: 241 EEMEINGSMTTPTATAPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300

Query: 301 DTSRYKVLPPECK 314
           DTSRYKV+PPECK
Sbjct: 301 DTSRYKVIPPECK 313

BLAST of Carg14462 vs. ExPASy TrEMBL
Match: A0A0A0KQA3 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G602120 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 8.0e-161
Identity = 277/313 (88.50%), Postives = 290/313 (92.65%), Query Frame = 0

Query: 1   MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG 60
           M  SRG+MEL LLSFIFLCMLFV PS    +RYYTPP+VPRLTDLVPRVS DQCFAK+FG
Sbjct: 1   MGFSRGIMELPLLSFIFLCMLFVAPS---CSRYYTPPSVPRLTDLVPRVSTDQCFAKIFG 60

Query: 61  ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120
           ASN++L NNGSSV+LTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD
Sbjct: 61  ASNIQLRNNGSSVDLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120

Query: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180
           VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN
Sbjct: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180

Query: 181 NYHTVFLVDNIPVRELKNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240
           NYHTVFLVDN+PVREL+NSE FYPSKPMSVFVTIWDGSEWATHGGKYPVDYK+APY ASF
Sbjct: 181 NYHTVFLVDNVPVRELRNSEVFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYTASF 240

Query: 241 EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300
           EEMEING + T   T PS SK NVS PDT E PEFIKLSQQQ++AMDWARRKLMFYSYCK
Sbjct: 241 EEMEINGGILTPKATVPSSSKANVSGPDTAEGPEFIKLSQQQVDAMDWARRKLMFYSYCK 300

Query: 301 DTSRYKVLPPECK 314
           DTSRYKVLPPECK
Sbjct: 301 DTSRYKVLPPECK 310

BLAST of Carg14462 vs. ExPASy TrEMBL
Match: N0DX87 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth29 PE=3 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 5.8e-159
Identity = 274/306 (89.54%), Postives = 286/306 (93.46%), Query Frame = 0

Query: 8   MELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFGASNMRLM 67
           MEL LLSFIFLCMLFV PS    +RYYTPP+VPRLTDLVPRVS DQCFAK+FGASN++L 
Sbjct: 1   MELPLLSFIFLCMLFVAPS---CSRYYTPPSVPRLTDLVPRVSTDQCFAKIFGASNIQLR 60

Query: 68  NNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHD 127
           NNGSSV+LTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHD
Sbjct: 61  NNGSSVDLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHD 120

Query: 128 EIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWNNYHTVFL 187
           EIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWNNYHTVFL
Sbjct: 121 EIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWNNYHTVFL 180

Query: 188 VDNIPVRELKNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASFEEMEING 247
           VDN+PVREL+NSE FYPSKPMSVFVTIWDGSEWATHGGKYPVDYK+APY ASFEEMEING
Sbjct: 181 VDNVPVRELRNSEVFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYTASFEEMEING 240

Query: 248 SMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCKDTSRYKV 307
            + T T T PS SK NVS PDT E PEFIKLSQQQ++AMDWARRKLMFYSYCKDTSRYKV
Sbjct: 241 GILTPTATVPSSSKANVSGPDTAEGPEFIKLSQQQVDAMDWARRKLMFYSYCKDTSRYKV 300

Query: 308 LPPECK 314
           LPPECK
Sbjct: 301 LPPECK 303

BLAST of Carg14462 vs. ExPASy TrEMBL
Match: A0A5D3CYT9 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00930 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 2.2e-158
Identity = 274/313 (87.54%), Postives = 289/313 (92.33%), Query Frame = 0

Query: 1   MEISRGVMELRLLSFIFLCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFG 60
           M  SR +MEL LLSFIFLCMLF VPS    +RYYT P+VPRLTDLVPRVS DQCFAK+FG
Sbjct: 1   MGFSRVIMELPLLSFIFLCMLFAVPS---CSRYYTAPSVPRLTDLVPRVSTDQCFAKIFG 60

Query: 61  ASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120
           ASN++L NNGSSV+LTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD
Sbjct: 61  ASNIQLRNNGSSVDLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNAD 120

Query: 121 VYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWN 180
           VYP SHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPS+KYHDYTIIWN
Sbjct: 121 VYPDSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSVKYHDYTIIWN 180

Query: 181 NYHTVFLVDNIPVRELKNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASF 240
           NYHTVFLVDN+PVREL+NSE FYP KPMSVFVTIWDGSEWATHGGKYPVDYK+APY ASF
Sbjct: 181 NYHTVFLVDNVPVRELRNSEVFYPLKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYTASF 240

Query: 241 EEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCK 300
           EEMEING + T T T PSGSK NVS PDT+E PEFIKLSQQQ++AMDWARRKLMFYSYCK
Sbjct: 241 EEMEINGGILTPTATVPSGSKANVSGPDTVEGPEFIKLSQQQVDAMDWARRKLMFYSYCK 300

Query: 301 DTSRYKVLPPECK 314
           DTSRYKVLPPECK
Sbjct: 301 DTSRYKVLPPECK 310

BLAST of Carg14462 vs. TAIR 10
Match: AT1G10550.1 (xyloglucan:xyloglucosyl transferase 33 )

HSP 1 Score: 387.5 bits (994), Expect = 1.0e-107
Identity = 190/304 (62.50%), Postives = 231/304 (75.99%), Query Frame = 0

Query: 18  LCMLFVVPSRGRFTRYYTPPNVPRLTDLVPRVSIDQCFAKLFGASNMRLMNNGSSVNLTL 77
           LC L +V S    +R +T PNV RLTD   +++I+  F++ FGA N+++  NGS   LTL
Sbjct: 13  LCSLSLVSSH---SRKFTTPNVTRLTDQFSKIAIENGFSRRFGAHNIQV--NGSLAKLTL 72

Query: 78  DKVSGAGLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSNADVYPHSHDEIDIELLGHD 137
           DK SGAGLVS+NKYHYGFFSA +KLP+G  SGVVVAFYLSNA+ YP SHDEIDIELLG  
Sbjct: 73  DKSSGAGLVSKNKYHYGFFSARLKLPAGFASGVVVAFYLSNAETYPKSHDEIDIELLGRS 132

Query: 138 KRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTIIWNNYHTVFLVDNIPVRELK 197
           +R DW IQTN+YANGS +TGREEKFY WFDP+  +HDYT+IWN++HTVFLVDNIPVR+  
Sbjct: 133 RRDDWTIQTNVYANGSTRTGREEKFYFWFDPTQAFHDYTLIWNSHHTVFLVDNIPVRQFP 192

Query: 198 NSEAF---YPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASFEEMEI------NGS 257
           N  AF   YPSKPMS++VT+WDGSEWAT GGKYPV+YKYAP+V S  ++E+      NGS
Sbjct: 193 NRGAFTSAYPSKPMSLYVTVWDGSEWATKGGKYPVNYKYAPFVVSVADVELSGCSVNNGS 252

Query: 258 MTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEAMDWARRKLMFYSYCKDTSRYKVL 313
            T S P + SG   ++SS D ++  +F  LS+ QI AMDWARRKLMFYSYC D  RYKV+
Sbjct: 253 STGSGPCTKSGG--SISSLDPVDGQDFATLSKNQINAMDWARRKLMFYSYCSDKPRYKVM 309

BLAST of Carg14462 vs. TAIR 10
Match: AT2G01850.1 (endoxyloglucan transferase A3 )

HSP 1 Score: 273.5 bits (698), Expect = 2.1e-73
Identity = 130/270 (48.15%), Postives = 185/270 (68.52%), Query Frame = 0

Query: 46  VPRVSIDQCFAKLFGASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSG 105
           +P  S ++ + +LFG  N+ +  +G SV LTLD+ +G+G VS + Y +GFFSASIKLPS 
Sbjct: 26  LPITSFEESYTQLFGDKNLFVHQDGKSVRLTLDERTGSGFVSNDYYLHGFFSASIKLPSD 85

Query: 106 LTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLW 165
            T+GVVVAFY+SN D+Y  +HDEID E LG+ + K+W +QTNIY NGS  +GREE++ LW
Sbjct: 86  YTAGVVVAFYMSNGDMYEKNHDEIDFEFLGNIREKEWRVQTNIYGNGSTHSGREERYNLW 145

Query: 166 FDPSLKYHDYTIIWNNYHTVFLVDNIPVRELKNSE---AFYPSKPMSVFVTIWDGSEWAT 225
           FDP+  +H Y+I+W++ H +F VDN+P+RE+K +      +PSKPMS++ TIWDGS+WAT
Sbjct: 146 FDPTEDFHQYSILWSDSHIIFFVDNVPIREVKRTAEMGGHFPSKPMSLYTTIWDGSKWAT 205

Query: 226 HGGKYPVDYKYAPYVASFEEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQ 285
           +GGKY V+YKYAPY+A F ++ ++G         P   + +  + + +   + I  SQ+ 
Sbjct: 206 NGGKYGVNYKYAPYIARFSDLVLHGCPVDPIEQFP---RCDEGAAEDMRAAQEITPSQR- 265

Query: 286 IEAMDWARRKLMFYSYCKDTSRYKVLPPEC 313
              MD  RR+LM YSYC D +RY V   EC
Sbjct: 266 -SKMDVFRRRLMTYSYCYDRARYNVALSEC 290

BLAST of Carg14462 vs. TAIR 10
Match: AT1G14720.1 (xyloglucan endotransglucosylase/hydrolase 28 )

HSP 1 Score: 263.8 bits (673), Expect = 1.7e-70
Identity = 126/270 (46.67%), Postives = 182/270 (67.41%), Query Frame = 0

Query: 46  VPRVSIDQCFAKLFGASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSG 105
           +P +  D+ + +LFG  N+ +  +G SV LTLD+ +G+G VS + Y +GFFS+SIKLP+ 
Sbjct: 26  LPLIQFDEGYTQLFGDQNLIVHRDGKSVRLTLDERTGSGFVSNDIYLHGFFSSSIKLPAD 85

Query: 106 LTSGVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLW 165
            ++GVV+AFYLSN D+Y  +HDEID E LG+ + ++W IQTNIY NGS   GREE++ LW
Sbjct: 86  YSAGVVIAFYLSNGDLYEKNHDEIDFEFLGNIRGREWRIQTNIYGNGSTHLGREERYNLW 145

Query: 166 FDPSLKYHDYTIIWNNYHTVFLVDNIPVRELKNSEAF---YPSKPMSVFVTIWDGSEWAT 225
           FDP+  +H Y+I+W+  H +F VDN+P+RE+K + +    +P+KPMS++ TIWDGS+WAT
Sbjct: 146 FDPTEDFHQYSILWSLSHIIFYVDNVPIREVKRTASMGGDFPAKPMSLYSTIWDGSKWAT 205

Query: 226 HGGKYPVDYKYAPYVASFEEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQ 285
            GGKY V+YKYAPYV+ F ++ ++G     T   PS     V +          ++++ Q
Sbjct: 206 DGGKYGVNYKYAPYVSQFTDLILHGCAVDPTEKFPSCKDEAVQNLRLAS-----EITESQ 265

Query: 286 IEAMDWARRKLMFYSYCKDTSRYKVLPPEC 313
              M+  R+K M YSYC D  RYKV+  EC
Sbjct: 266 RNKMEIFRQKHMTYSYCYDHMRYKVVLSEC 290

BLAST of Carg14462 vs. TAIR 10
Match: AT1G32170.1 (xyloglucan endotransglucosylase/hydrolase 30 )

HSP 1 Score: 253.1 bits (645), Expect = 3.0e-67
Identity = 119/267 (44.57%), Postives = 173/267 (64.79%), Query Frame = 0

Query: 49  VSIDQCFAKLFGASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTS 108
           +S ++  + LFG +N+    +  SV L LD+ +G+G +S N Y +GF+S+ IKLP+  T+
Sbjct: 30  LSFEESLSPLFGDANLVRSPDDLSVRLLLDRYTGSGFISSNMYQHGFYSSMIKLPADYTA 89

Query: 109 GVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDP 168
           GVVVAFY SN DV+  +HDE+DIE LG+ K K W  QTN+Y NGS   GREE++ LWFDP
Sbjct: 90  GVVVAFYTSNGDVFEKTHDELDIEFLGNIKGKPWRFQTNLYGNGSTHRGREERYRLWFDP 149

Query: 169 SLKYHDYTIIWNNYHTVFLVDNIPVRELKNSEAF---YPSKPMSVFVTIWDGSEWATHGG 228
           S ++H Y+I+W  +  +F VD++P+RE+  ++A    YP+KPM+++ TIWD S+WAT GG
Sbjct: 150 SKEFHRYSILWTPHKIIFWVDDVPIREVIRNDAMGADYPAKPMALYATIWDASDWATSGG 209

Query: 229 KYPVDYKYAPYVASFEEMEINGSMTTSTPTSPSGSKTNVSSPDTLERPEFIKLSQQQIEA 288
           KY  +YK+AP+VA F+   ++G         P     +V   D LE  ++  ++  Q  A
Sbjct: 210 KYKANYKFAPFVAEFKSFSLDGCSVDPIQEVPMDCSDSV---DFLESQDYSSINSHQRAA 269

Query: 289 MDWARRKLMFYSYCKDTSRYKVLPPEC 313
           M   R++ M+YSYC DT RY    PEC
Sbjct: 270 MRRFRQRFMYYSYCYDTLRYPEPLPEC 293

BLAST of Carg14462 vs. TAIR 10
Match: AT4G18990.1 (xyloglucan endotransglucosylase/hydrolase 29 )

HSP 1 Score: 246.9 bits (629), Expect = 2.2e-65
Identity = 121/275 (44.00%), Postives = 175/275 (63.64%), Query Frame = 0

Query: 49  VSIDQCFAKLFGASNMRLMNNGSSVNLTLDKVSGAGLVSRNKYHYGFFSASIKLPSGLTS 108
           +  D+  + LFG  N+    +  SV L LDK +G+G +S + Y +GFFS+ IKLP   T+
Sbjct: 38  IFFDEGLSHLFGEGNLIRSPDDRSVRLLLDKYTGSGFISSSMYQHGFFSSLIKLPGAYTA 97

Query: 109 GVVVAFYLSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDP 168
           G+VVAFY SN DV+   HDE+DIE LG+ + K W  QTN+Y NGS   GREE++ LWFDP
Sbjct: 98  GIVVAFYTSNGDVFVKDHDELDIEFLGNLEGKPWRFQTNMYGNGSTNRGREERYRLWFDP 157

Query: 169 SLKYHDYTIIWNNYHTVFLVDNIPVRELKNSEAF---YPSKPMSVFVTIWDGSEWATHGG 228
           S ++H Y+I+W     +F VD++P+RE+   E     YP KPMS++ TIWD S WAT GG
Sbjct: 158 SKEFHRYSILWTPTKIIFWVDDVPIREILRKEEMNGDYPQKPMSLYATIWDASSWATSGG 217

Query: 229 KYPVDYKYAPYVASFEEMEING-SMTTSTP----TSPSGSKTNVS---SPDTLERPEFIK 288
           K+ VDY ++P+V+ F+++ ++G +++ S P     +  G+  N++   S   L   ++  
Sbjct: 218 KFGVDYTFSPFVSEFKDIALDGCNVSDSFPGENNNNNIGNYNNINCSVSDQFLMSNDYST 277

Query: 289 LSQQQIEAMDWARRKLMFYSYCKDTSRYKVLPPEC 313
           +S +Q  AM   R + M+YSYC DT RY V PPEC
Sbjct: 278 ISPKQATAMRRFRERYMYYSYCYDTIRYSVPPPEC 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7012822.15.9e-182100.00putative xyloglucan endotransglucosylase/hydrolase protein 33, partial [Cucurbit... [more]
XP_022945421.11.3e-18199.68probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucurbita moschat... [more]
XP_022966887.17.4e-17797.12probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucurbita maxima][more]
XP_038892286.12.1e-16390.10probable xyloglucan endotransglucosylase/hydrolase protein 33 isoform X2 [Beninc... [more]
XP_004135238.11.7e-16088.50probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucumis sativus] ... [more]
Match NameE-valueIdentityDescription
Q8LC451.4e-10662.50Probable xyloglucan endotransglucosylase/hydrolase protein 33 OS=Arabidopsis tha... [more]
Q8LDS23.0e-7248.15Probable xyloglucan endotransglucosylase/hydrolase protein 27 OS=Arabidopsis tha... [more]
Q389092.4e-6946.67Probable xyloglucan endotransglucosylase/hydrolase protein 28 OS=Arabidopsis tha... [more]
Q389084.2e-6644.57Probable xyloglucan endotransglucosylase/hydrolase protein 30 OS=Arabidopsis tha... [more]
Q8L7H33.0e-6444.00Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis tha... [more]
Match NameE-valueIdentityDescription
A0A6J1G0T56.3e-18299.68Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1HP763.6e-17797.12Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A0A0KQA38.0e-16188.50Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G60... [more]
N0DX875.8e-15989.54Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth29 PE... [more]
A0A5D3CYT92.2e-15887.54Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo var. makuwa OX=1194695... [more]
Match NameE-valueIdentityDescription
AT1G10550.11.0e-10762.50xyloglucan:xyloglucosyl transferase 33 [more]
AT2G01850.12.1e-7348.15endoxyloglucan transferase A3 [more]
AT1G14720.11.7e-7046.67xyloglucan endotransglucosylase/hydrolase 28 [more]
AT1G32170.13.0e-6744.57xyloglucan endotransglucosylase/hydrolase 30 [more]
AT4G18990.12.2e-6544.00xyloglucan endotransglucosylase/hydrolase 29 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 60..232
e-value: 2.2E-49
score: 167.4
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 31..240
score: 26.205481
IPR010713Xyloglucan endo-transglycosylase, C-terminalPFAMPF06955XET_Ccoord: 277..312
e-value: 2.3E-14
score: 53.0
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 23..313
e-value: 3.6E-103
score: 342.6
NoneNo IPR availableGENE3D2.60.120.200coord: 37..312
e-value: 2.1E-86
score: 291.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 248..271
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 248..269
NoneNo IPR availablePANTHERPTHR31062:SF144XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 33-RELATEDcoord: 9..313
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 9..313
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 55..312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14462-RACarg14462-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0009831 plant-type cell wall modification involved in multidimensional cell growth
biological_process GO:0010411 xyloglucan metabolic process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006073 cellular glucan metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
cellular_component GO:0005887 integral component of plasma membrane
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity