Carg14286 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg14286
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type domain-containing protein
Location	Carg_Chr04: 10553767 .. 10555025 (+)
RNA-Seq Expression	Carg14286
Synteny	Carg14286

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTATTTTTTTTAAAAAAATAATTTTGATTTGAAAACTTGCTAATGGGCCTTGGTCCAAAGCAACCTTGAGAGCCTCTAAATGAAACGGACTCGGGCTGAAGAAGGGAGGTGACCTAATCGTCCGGATCTTACATTTTTCTGCCAATCTCTGTCTAAGCTCCGATGAACGAACATGGAGGAACCCATCGGAGAGAGACAGAAATGCCGATATCAGAATTGGTGAGGGACAGACGATGGGCGGAATGAAGCGGCGCGATCAAAGTTGGGTAAGAAGCACGCAGCATTAAATTTGCAGCAATCAATAGGGTTTAATGCTTGTCTTGATTGTTAACATGCTTAGTTATTGGGTTTTGGGTATTCTAAGGAAGGAAGGAAGGTCTGACTTGGAAACGATGCAATCCCCGACTAAGAAACAATGGATAAACACTAAACACAAGGTATCCTTTTCTTGTCTTAAAGTGTCGAAGCTTTGAAGTTTGAAATCAAAGCAAGGTTTTACCCACATCTCCCTCTTTCCCTCATTTTTTAGATGTCTGGACACGGCCTTTCCACAAGTATATCTCTTCTTTTCTCAACATCATCTGGCTGCTGCTCTGTTCTGTCGAGTTGGAAGGAGAAACAATGTGGCAACAACAGCCCAAAAGGAACTCGTTTAAAGAATCTGTCAAGGCTGTTGAAGCTGATATACGACGTGCAAATGCCTTGTAAGATCTTCTATTCGGGTTCTATTCTCTGTCAGATATGTTCAAATCCCTTGTTAATAGATATTGTCTTCTTTAGACTTTCTCTTTAAAGCTTTCTCCTAATAGATATGATCTTCCTTATATTTTCACTTTAGAGCTTTCTCCTAGTAGATATTGTCTTCTTTAGTTGGGAGAAGTTTTCATATCCTTATAAGGAATGAATTTCATAAATTCCACGTCAGAATAATACCCTACTTTATGTTCCAGAACCCAAAAGATCTTCATGGTTCTTAACTTCTGATACCCGAGCCCTGTTGTTCCACTACTTGAACTACGACTTCTCATGGGTTTGGTTTTGCATTTGTAGGGCAGCTATGTTCTCGAGAGATTACGGTGGATTTTGCATTCAAATGAGATTGCAATACAGCCCATTTGCTCTCTTTTTTCTCCATTGGATTGAATGGATGGATTTCAGTTGCACAGATCCTCTTCCAAGTTTCTTGGGCCTTTTCCACATACTCTTGTACAAGGTGCTTCCCTCAACTCTTGCAGCATATTCACAACCTGAAATCTAA

mRNA sequence

ATTATTTTTTTTAAAAAAATAATTTTGATTTGAAAACTTGCTAATGGGCCTTGGTCCAAAGCAACCTTGAGAGCCTCTAAATGAAACGGACTCGGGCTGAAGAAGGGAGGTGACCTAATCGTCCGGATCTTACATTTTTCTGCCAATCTCTGTCTAAGCTCCGATGAACGAACATGGAGGAACCCATCGGAGAGAGACAGAAATGCCGATATCAGAATTGGTGAGGGACAGACGATGGGCGGAATGAAGCGGCGCGATCAAAGTTGGGAAGGAAGGTCTGACTTGGAAACGATGCAATCCCCGACTAAGAAACAATGGATAAACACTAAACACAAGATGTCTGGACACGGCCTTTCCACAAGTATATCTCTTCTTTTCTCAACATCATCTGGCTGCTGCTCTGTTCTGTCGAGTTGGAAGGAGAAACAATGTGGCAACAACAGCCCAAAAGGAACTCGTTTAAAGAATCTGGCAGCTATGTTCTCGAGAGATTACGGTGGATTTTGCATTCAAATGAGATTGCAATACAGCCCATTTGCTCTCTTTTTTCTCCATTGGATTGAATGGATGGATTTCAGTTGCACAGATCCTCTTCCAAGTTTCTTGGGCCTTTTCCACATACTCTTGTACAAGGTGCTTCCCTCAACTCTTGCAGCATATTCACAACCTGAAATCTAA

Coding sequence (CDS)

ATGGGCGGAATGAAGCGGCGCGATCAAAGTTGGGAAGGAAGGTCTGACTTGGAAACGATGCAATCCCCGACTAAGAAACAATGGATAAACACTAAACACAAGATGTCTGGACACGGCCTTTCCACAAGTATATCTCTTCTTTTCTCAACATCATCTGGCTGCTGCTCTGTTCTGTCGAGTTGGAAGGAGAAACAATGTGGCAACAACAGCCCAAAAGGAACTCGTTTAAAGAATCTGGCAGCTATGTTCTCGAGAGATTACGGTGGATTTTGCATTCAAATGAGATTGCAATACAGCCCATTTGCTCTCTTTTTTCTCCATTGGATTGAATGGATGGATTTCAGTTGCACAGATCCTCTTCCAAGTTTCTTGGGCCTTTTCCACATACTCTTGTACAAGGTGCTTCCCTCAACTCTTGCAGCATATTCACAACCTGAAATCTAA

Protein sequence

MGGMKRRDQSWEGRSDLETMQSPTKKQWINTKHKMSGHGLSTSISLLFSTSSGCCSVLSSWKEKQCGNNSPKGTRLKNLAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLYKVLPSTLAAYSQPEI

Homology

[Close]

BLAST of Carg14286 vs. NCBI nr
Match: KAG7032335.1 (hypothetical protein SDJN02_06380 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 307.0 bits (785), Expect = 8.8e-80
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGGMKRRDQSWEGRSDLETMQSPTKKQWINTKHKMSGHGLSTSISLLFSTSSGCCSVLSS 60
           MGGMKRRDQSWEGRSDLETMQSPTKKQWINTKHKMSGHGLSTSISLLFSTSSGCCSVLSS
Sbjct: 1   MGGMKRRDQSWEGRSDLETMQSPTKKQWINTKHKMSGHGLSTSISLLFSTSSGCCSVLSS 60

Query: 61  WKEKQCGNNSPKGTRLKNLAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPL 120
           WKEKQCGNNSPKGTRLKNLAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPL
Sbjct: 61  WKEKQCGNNSPKGTRLKNLAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPL 120

Query: 121 PSFLGLFHILLYKVLPSTLAAYSQPEI 148
           PSFLGLFHILLYKVLPSTLAAYSQPEI
Sbjct: 121 PSFLGLFHILLYKVLPSTLAAYSQPEI 147

[Close]

BLAST of Carg14286 vs. NCBI nr
Match: XP_022998299.1 (uncharacterized protein LOC111492969 isoform X2 [Cucurbita maxima])

HSP 1 Score: 171.4 bits (433), Expect = 5.8e-39
Identity = 93/138 (67.39%), Postives = 100/138 (72.46%), Query Frame = 0


Query: 12  EGRSDLETMQSPTKKQWINTKHKMSGHGLSTSISLLFSTS-SGCCSVLSSWKEKQCGNNS 71
           EG+SDLETMQSPTKKQWINTK KMSGHGLST+ISLL        CSV    + ++     
Sbjct: 2   EGKSDLETMQSPTKKQWINTKQKMSGHGLSTNISLLSQHHLLLLCSV--KLEGERMRQQQ 61

Query: 72  PKGTRLKN--------------LAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSC 131
           PK    K               LAAMFSR+YGGFCIQMRLQYSPFALFFLHWIEWMDFSC
Sbjct: 62  PKRISFKESVKAVEADIRRANALAAMFSREYGGFCIQMRLQYSPFALFFLHWIEWMDFSC 121

Query: 132 TDPLPSFLGLFHILLYKV 135
           TDPLPSFLGLFH+LLYKV
Sbjct: 122 TDPLPSFLGLFHVLLYKV 137

[Close]

BLAST of Carg14286 vs. NCBI nr
Match: XP_022998292.1 (uncharacterized protein LOC111492969 isoform X1 [Cucurbita maxima])

HSP 1 Score: 171.4 bits (433), Expect = 5.8e-39
Identity = 93/138 (67.39%), Postives = 100/138 (72.46%), Query Frame = 0


Query: 12  EGRSDLETMQSPTKKQWINTKHKMSGHGLSTSISLLFSTS-SGCCSVLSSWKEKQCGNNS 71
           EG+SDLETMQSPTKKQWINTK KMSGHGLST+ISLL        CSV    + ++     
Sbjct: 20  EGKSDLETMQSPTKKQWINTKQKMSGHGLSTNISLLSQHHLLLLCSV--KLEGERMRQQQ 79

Query: 72  PKGTRLKN--------------LAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSC 131
           PK    K               LAAMFSR+YGGFCIQMRLQYSPFALFFLHWIEWMDFSC
Sbjct: 80  PKRISFKESVKAVEADIRRANALAAMFSREYGGFCIQMRLQYSPFALFFLHWIEWMDFSC 139

Query: 132 TDPLPSFLGLFHILLYKV 135
           TDPLPSFLGLFH+LLYKV
Sbjct: 140 TDPLPSFLGLFHVLLYKV 155

[Close]

BLAST of Carg14286 vs. NCBI nr
Match: KAG6601571.1 (E3 ubiquitin-protein ligase AIRP2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 144.8 bits (364), Expect = 5.8e-31
Identity = 75/108 (69.44%), Postives = 83/108 (76.85%), Query Frame = 0


Query: 12  EGRSDLETMQSPTKKQWINTKHKMSGHGLSTSISLLFSTSSGCCSVLSSWKEKQCGNNSP 71
           EGRSDLETMQSPTKKQWINTKHKMSGHGLST+ISLLFSTSSGCCSVLSSWKEK+CGNNSP
Sbjct: 18  EGRSDLETMQSPTKKQWINTKHKMSGHGLSTNISLLFSTSSGCCSVLSSWKEKECGNNSP 77

Query: 72  KGTRLKNLAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEW--MDFSCT 118
           KGTRLKNL+ +    Y    +QM  Q     +  L  + W    +SCT
Sbjct: 78  KGTRLKNLSRLLKLIYD---VQMPWQLCSREITILFQVSWAFSTYSCT 122

[Close]

BLAST of Carg14286 vs. NCBI nr
Match: XP_022956579.1 (uncharacterized protein LOC111458275 [Cucurbita moschata] >XP_022956580.1 uncharacterized protein LOC111458275 [Cucurbita moschata] >XP_022956581.1 uncharacterized protein LOC111458275 [Cucurbita moschata])

HSP 1 Score: 125.6 bits (314), Expect = 3.6e-25
Identity = 57/60 (95.00%), Postives = 57/60 (95.00%), Query Frame = 0


Query: 75  RLKNLAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLYKV 134
           R   LAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLYKV
Sbjct: 24  RANALAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLYKV 83

[Close]

BLAST of Carg14286 vs. ExPASy TrEMBL
Match: A0A6J1K7J3 (uncharacterized protein LOC111492969 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492969 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.8e-39
Identity = 93/138 (67.39%), Postives = 100/138 (72.46%), Query Frame = 0


Query: 12  EGRSDLETMQSPTKKQWINTKHKMSGHGLSTSISLLFSTS-SGCCSVLSSWKEKQCGNNS 71
           EG+SDLETMQSPTKKQWINTK KMSGHGLST+ISLL        CSV    + ++     
Sbjct: 20  EGKSDLETMQSPTKKQWINTKQKMSGHGLSTNISLLSQHHLLLLCSV--KLEGERMRQQQ 79

Query: 72  PKGTRLKN--------------LAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSC 131
           PK    K               LAAMFSR+YGGFCIQMRLQYSPFALFFLHWIEWMDFSC
Sbjct: 80  PKRISFKESVKAVEADIRRANALAAMFSREYGGFCIQMRLQYSPFALFFLHWIEWMDFSC 139

Query: 132 TDPLPSFLGLFHILLYKV 135
           TDPLPSFLGLFH+LLYKV
Sbjct: 140 TDPLPSFLGLFHVLLYKV 155

[Close]

BLAST of Carg14286 vs. ExPASy TrEMBL
Match: A0A6J1KDZ0 (uncharacterized protein LOC111492969 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492969 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.8e-39
Identity = 93/138 (67.39%), Postives = 100/138 (72.46%), Query Frame = 0


Query: 12  EGRSDLETMQSPTKKQWINTKHKMSGHGLSTSISLLFSTS-SGCCSVLSSWKEKQCGNNS 71
           EG+SDLETMQSPTKKQWINTK KMSGHGLST+ISLL        CSV    + ++     
Sbjct: 2   EGKSDLETMQSPTKKQWINTKQKMSGHGLSTNISLLSQHHLLLLCSV--KLEGERMRQQQ 61

Query: 72  PKGTRLKN--------------LAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSC 131
           PK    K               LAAMFSR+YGGFCIQMRLQYSPFALFFLHWIEWMDFSC
Sbjct: 62  PKRISFKESVKAVEADIRRANALAAMFSREYGGFCIQMRLQYSPFALFFLHWIEWMDFSC 121

Query: 132 TDPLPSFLGLFHILLYKV 135
           TDPLPSFLGLFH+LLYKV
Sbjct: 122 TDPLPSFLGLFHVLLYKV 137

[Close]

BLAST of Carg14286 vs. ExPASy TrEMBL
Match: A0A6J1GWY5 (uncharacterized protein LOC111458275 OS=Cucurbita moschata OX=3662 GN=LOC111458275 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.8e-25
Identity = 57/60 (95.00%), Postives = 57/60 (95.00%), Query Frame = 0


Query: 75  RLKNLAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLYKV 134
           R   LAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLYKV
Sbjct: 24  RANALAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLYKV 83

[Close]

BLAST of Carg14286 vs. ExPASy TrEMBL
Match: A0A5A7SXP9 (Peroxisome biogenesis factor 10-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001870 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 1.0e-20
Identity = 48/56 (85.71%), Postives = 51/56 (91.07%), Query Frame = 0


Query: 79  LAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLYKV 135
           LAAM  R+YGGFCIQMRL YSPFA F LHWIEWMDF+CTDP+PSFLGLFHILLYKV
Sbjct: 28  LAAMLPREYGGFCIQMRLHYSPFAPFLLHWIEWMDFNCTDPVPSFLGLFHILLYKV 83

[Close]

BLAST of Carg14286 vs. ExPASy TrEMBL
Match: A0A1S3BDT3 (peroxisome biogenesis factor 10-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488931 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 1.0e-20
Identity = 48/56 (85.71%), Postives = 51/56 (91.07%), Query Frame = 0


Query: 79  LAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLYKV 135
           LAAM  R+YGGFCIQMRL YSPFA F LHWIEWMDF+CTDP+PSFLGLFHILLYKV
Sbjct: 28  LAAMLPREYGGFCIQMRLHYSPFAPFLLHWIEWMDFNCTDPVPSFLGLFHILLYKV 83

[Close]

BLAST of Carg14286 vs. TAIR 10
Match: AT1G24440.1 (RING/U-box superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 2.4e-06
Identity = 26/56 (46.43%), Postives = 33/56 (58.93%), Query Frame = 0


Query: 79  LAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLYKV 135
           LAA       G  +QM+L  S  A FF+  ++WMDFSC   LP +   FHIL+YKV
Sbjct: 27  LAAEIPMGKSGVRLQMKLVCSNLAPFFIFLLQWMDFSCL--LPRYFDFFHILIYKV 80

[Close]

BLAST of Carg14286 vs. TAIR 10
Match: AT5G58787.1 (RING/U-box superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 7.6e-05
Identity = 24/54 (44.44%), Postives = 31/54 (57.41%), Query Frame = 0


Query: 79  LAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLY 133
           LA  + R+  G  +QMRL YSP A FFL  ++W D      L  FLGL  +L+Y
Sbjct: 23  LALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCK----LAGFLGLLRVLIY 72

[Close]

BLAST of Carg14286 vs. TAIR 10
Match: AT5G58787.2 (RING/U-box superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 7.6e-05
Identity = 24/54 (44.44%), Postives = 31/54 (57.41%), Query Frame = 0


Query: 79  LAAMFSRDYGGFCIQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLY 133
           LA  + R+  G  +QMRL YSP A FFL  ++W D      L  FLGL  +L+Y
Sbjct: 23  LALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCK----LAGFLGLLRVLIY 72

[Close]

BLAST of Carg14286 vs. TAIR 10
Match: AT1G13195.1 (RING/U-box superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 1.3e-04
Identity = 19/43 (44.19%), Postives = 29/43 (67.44%), Query Frame = 0


Query: 92  IQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLYKV 135
           +QM+L +S FA   L  + W+D S +  +P +L LFH+L+YKV
Sbjct: 46  LQMKLVHSNFASLLLFLLRWIDLSSSCLIPRYLNLFHVLVYKV 88

[Close]

BLAST of Carg14286 vs. TAIR 10
Match: AT1G13195.2 (RING/U-box superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 1.3e-04
Identity = 19/43 (44.19%), Postives = 29/43 (67.44%), Query Frame = 0


Query: 92  IQMRLQYSPFALFFLHWIEWMDFSCTDPLPSFLGLFHILLYKV 135
           +QM+L +S FA   L  + W+D S +  +P +L LFH+L+YKV
Sbjct: 8   LQMKLVHSNFASLLLFLLRWIDLSSSCLIPRYLNLFHVLVYKV 50

The following BLAST results are available for this feature:

BLAST of Carg14286 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7032335.1	8.8e-80	100.00	hypothetical protein SDJN02_06380 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022998299.1	5.8e-39	67.39	uncharacterized protein LOC111492969 isoform X2 [Cucurbita maxima]	[more]
XP_022998292.1	5.8e-39	67.39	uncharacterized protein LOC111492969 isoform X1 [Cucurbita maxima]	[more]
KAG6601571.1	5.8e-31	69.44	E3 ubiquitin-protein ligase AIRP2, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022956579.1	3.6e-25	95.00	uncharacterized protein LOC111458275 [Cucurbita moschata] >XP_022956580.1 unchar...	[more]

BLAST of Carg14286 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg14286 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K7J3	2.8e-39	67.39	uncharacterized protein LOC111492969 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1KDZ0	2.8e-39	67.39	uncharacterized protein LOC111492969 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1GWY5	1.8e-25	95.00	uncharacterized protein LOC111458275 OS=Cucurbita moschata OX=3662 GN=LOC1114582...	[more]
A0A5A7SXP9	1.0e-20	85.71	Peroxisome biogenesis factor 10-like isoform X1 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3BDT3	1.0e-20	85.71	peroxisome biogenesis factor 10-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Carg14286 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24440.1	2.4e-06	46.43	RING/U-box superfamily protein	[more]
AT5G58787.1	7.6e-05	44.44	RING/U-box superfamily protein	[more]
AT5G58787.2	7.6e-05	44.44	RING/U-box superfamily protein	[more]
AT1G13195.1	1.3e-04	44.19	RING/U-box superfamily protein	[more]
AT1G13195.2	1.3e-04	44.19	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	PANTHER	PTHR15315:SF22	RING-H2 FINGER PROTEIN ATL47-LIKE	coord: 77..135
None	No IPR available	PANTHER	PTHR15315	RING FINGER PROTEIN 41, 151	coord: 77..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg14286-RA	Carg14286-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0061630	ubiquitin protein ligase activity