Carg14030 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg14030
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Bidirectional sugar transporter SWEET
Location	Carg_Chr02: 6470021 .. 6471734 (+)
RNA-Seq Expression	Carg14030
Synteny	Carg14030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGATCTCATCATAGCATTCGAATGATTGTTGGAATCTTTGGTATGCTTTGTTTTTTAATGAATTTCTTTGAACATTTCTGTTCAAAATGACCATTTTTTTTTTCTTTGTGTTTCTCTATGCAGGAAATGGTGCTTCTTTGCTGCTTTATTGTGTTCCCATGTAATGAAATCTGCCAACTTTGCTTTGTTCTTATTGTTTGGAGACAAATAATGGTTTGAATCTTATCTAATTCGAACGTTTTCGTGCCTAAAACTCGATAAAAAAAGACACCATTTTAGACGTTTTTGCTATTGAATTCGATATCAACTTGAAGTGGACAAGCATTTGAAGGCATGCCCACATAGCTAAGTAATAAGTTGGGTGTGTTTATGCAGATTGACATTTTGGAGGGTTGTAAAGAAGAAGAGCACAGAGGAGTTTTCATGTGTTCCATACATTGTGGCGCTGTTGAATTGTCTTCTTTATACTTGGTATGCTTTACCAGTTGTGAGCAATGGATGGGAGAACTTCCCTGTTGTCACCATTAATGGCTTTGGTGTTCTTCTTGAGTTCTCTTTCATTTTCATCTACGTCTGGTTTTCCTCTCCAAAACGAAAGGCAAGTTTTTGTCTACGATCCCATTTGATAAACATTTTGTGTTTTAATTTCTTGTTTTCTTTTCGAACATGAATTTGTTCAGTTACTCGAAGTTCGTTTGTTTTGAAAACAGTAGAGGAAAGTCGCCTCTACTACTGCAAATTTCTATGAAATTTGGTAGGAGGGTCTCAAGTTTTACTATTTTAGACGATAATCTCACTTGAAATTTTCTCAAATCCGCTAAGTAAAATCATATGAATTTAATATTCGAAGTAAAAACGAATGATCTTAGTGAAACAATGAATATGTTTAAGCTCAAAATTTATAAGAATTATTAATATGGTTTCTTTTAGGCTTAAAAAAGTTCTAAAATTATCTGCAGAAGAAGGTGGCTTTGAGATTGGTGGGAGTTGTTATAGTGTTCAGCTGTGTGGGGATGATCTCTACTTTTGCTTTGAAAAGTCACCATCTTCGGAAGCTTTTTGTGGGATGCATTGGTCTTGTTGCTTCTGTTGCCATGTATGGCTCTCCATTGGTAGCTATGGTAAGCTCGCGCCAAAACCCTGTAGTCAAAACTTGCTTCGACATTCTTGTTTCGTGTTTCTTGTTTTCACTTTTGCTCGGACGTTACCAAACGACCACCTAATTTCATCACAACAGTTGATACCTACAAAAGAGACAATGTTTGTGTTTGTCACTAAAAGTTTATTTGTCATGCAGAAACAAGTAATAAAAACAAAGAGTGTTGAGTTCATGCCATTTTATTTGTCATTCTTCTCCTTCTCTGCAAGCTCTTTGTGGTTAGCTTATGGACTCCTCAGCCATGACCTCTTCCTTGCAGTCAGTATTACCAAATCTCTCCTTTTTTTTGGTTTTTTTTTCTTTTTCCATATGAACTTGAAAGTAAATTAACTCATGAAACTTTGAATGGATTTGCAGTCTCCAAATCTAGTGGGAAGCCCATTAGGGTTGCTACAGCTTGTGTTGTATTGCATCTATAGGAATAAGGGAAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGATGTGTTAAAGAGGCTGTGGCCAATTGGGACATGGAAAAGAATAATGCAAAACAAATTCCACACAAAAATGGCTCTCAAGTTTAG

mRNA sequence

ATGGGATCTCATCATAGCATTCGAATGATTGTTGGAATCTTTGGAAATGGTGCTTCTTTGCTGCTTTATTGTGTTCCCATATTGACATTTTGGAGGGTTGTAAAGAAGAAGAGCACAGAGGAGTTTTCATGTGTTCCATACATTGTGGCGCTGTTGAATTGTCTTCTTTATACTTGGTATGCTTTACCAGTTGTGAGCAATGGATGGGAGAACTTCCCTGTTGTCACCATTAATGGCTTTGGTGTTCTTCTTGAGTTCTCTTTCATTTTCATCTACGTCTGGTTTTCCTCTCCAAAACGAAAGGCAATTACTCGAAGTTCGTTTGTTTTGAAAACAAAGAAGGTGGCTTTGAGATTGGTGGGAGTTGTTATAGTGTTCAGCTGTGTGGGGATGATCTCTACTTTTGCTTTGAAAAGTCACCATCTTCGGAAGCTTTTTGTGGGATGCATTGGTCTTGTTGCTTCTGTTGCCATGTATGGCTCTCCATTGGTAGCTATGCTTGTGTTGTATTGCATCTATAGGAATAAGGGAAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGATGTGTTAAAGAGGCTGTGGCCAATTGGGACATGGAAAAGAATAATGCAAAACAAATTCCACACAAAAATGGCTCTCAAGTTTAG

Coding sequence (CDS)

ATGGGATCTCATCATAGCATTCGAATGATTGTTGGAATCTTTGGAAATGGTGCTTCTTTGCTGCTTTATTGTGTTCCCATATTGACATTTTGGAGGGTTGTAAAGAAGAAGAGCACAGAGGAGTTTTCATGTGTTCCATACATTGTGGCGCTGTTGAATTGTCTTCTTTATACTTGGTATGCTTTACCAGTTGTGAGCAATGGATGGGAGAACTTCCCTGTTGTCACCATTAATGGCTTTGGTGTTCTTCTTGAGTTCTCTTTCATTTTCATCTACGTCTGGTTTTCCTCTCCAAAACGAAAGGCAATTACTCGAAGTTCGTTTGTTTTGAAAACAAAGAAGGTGGCTTTGAGATTGGTGGGAGTTGTTATAGTGTTCAGCTGTGTGGGGATGATCTCTACTTTTGCTTTGAAAAGTCACCATCTTCGGAAGCTTTTTGTGGGATGCATTGGTCTTGTTGCTTCTGTTGCCATGTATGGCTCTCCATTGGTAGCTATGCTTGTGTTGTATTGCATCTATAGGAATAAGGGAAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGATGTGTTAAAGAGGCTGTGGCCAATTGGGACATGGAAAAGAATAATGCAAAACAAATTCCACACAAAAATGGCTCTCAAGTTTAG

Protein sequence

MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWYALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLVGVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAMLVLYCIYRNKGKEEEEEEEEEEEGCVKEAVANWDMEKNNAKQIPHKNGSQV

Homology

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BLAST of Carg14030 vs. NCBI nr
Match: KAG7035735.1 (Bidirectional sugar transporter SWEET3b, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 430.6 bits (1106), Expect = 7.8e-117
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60
           MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY
Sbjct: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60

Query: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLV 120
           ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLV
Sbjct: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLV 120

Query: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAMLVLYCIYRNKGKEE 180
           GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAMLVLYCIYRNKGKEE
Sbjct: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAMLVLYCIYRNKGKEE 180

Query: 181 EEEEEEEEEGCVKEAVANWDMEKNNAKQIPHKNGSQV 218
           EEEEEEEEEGCVKEAVANWDMEKNNAKQIPHKNGSQV
Sbjct: 181 EEEEEEEEEGCVKEAVANWDMEKNNAKQIPHKNGSQV 217

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BLAST of Carg14030 vs. NCBI nr
Match: XP_022958511.1 (bidirectional sugar transporter SWEET3b-like [Cucurbita moschata])

HSP 1 Score: 363.2 bits (931), Expect = 1.5e-96
Identity = 200/270 (74.07%), Postives = 201/270 (74.44%), Query Frame = 0


Query: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60
           MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY
Sbjct: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60

Query: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLV 120
           ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRK           KKVALRLV
Sbjct: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRK-----------KKVALRLV 120

Query: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM-------------- 180
           GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM              
Sbjct: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAMKQVIKTKSVEFMPF 180

Query: 181 ---------------------------------------LVLYCIYRNKGKEEEEEEEEE 218
                                                  LVLYCIYRNKGK     EEEE
Sbjct: 181 YLSFFSFSASSLWLAYGLLSHDLFLASPNLVGSPLGLLQLVLYCIYRNKGK-----EEEE 240

[Close]

BLAST of Carg14030 vs. NCBI nr
Match: XP_023534737.1 (bidirectional sugar transporter SWEET3b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 361.3 bits (926), Expect = 5.8e-96
Identity = 199/270 (73.70%), Postives = 200/270 (74.07%), Query Frame = 0


Query: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60
           MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY
Sbjct: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60

Query: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLV 120
           ALPVVSNGWENFPVVTINGFGVLLEFSFIFIY WFSSPKRK           KKVALRLV
Sbjct: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYFWFSSPKRK-----------KKVALRLV 120

Query: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM-------------- 180
           GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM              
Sbjct: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAMKQVIKTKSVEFMPF 180

Query: 181 ---------------------------------------LVLYCIYRNKGKEEEEEEEEE 218
                                                  LVLYCIYRNKGK     EEEE
Sbjct: 181 YLSFFSFSASSLWLAYGLLSHDLFLASPNLVGSPLGLLQLVLYCIYRNKGK-----EEEE 240

[Close]

BLAST of Carg14030 vs. NCBI nr
Match: XP_022995390.1 (bidirectional sugar transporter SWEET3b-like [Cucurbita maxima])

HSP 1 Score: 352.8 bits (904), Expect = 2.1e-93
Identity = 194/270 (71.85%), Postives = 196/270 (72.59%), Query Frame = 0


Query: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60
           MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY
Sbjct: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60

Query: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLV 120
           ALPVVSNGWENFPVVTINGFGVLLEFSFIFIY WFSSPKRK           K+V LRLV
Sbjct: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYFWFSSPKRK-----------KQVTLRLV 120

Query: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM-------------- 180
           GVV VFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM              
Sbjct: 121 GVVTVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAMKQVIKTKSVEYMPF 180

Query: 181 ---------------------------------------LVLYCIYRNKGKEEEEEEEEE 218
                                                  LVLYCIYRNKGK       EE
Sbjct: 181 YLSFFSFSASSLWLAYGLLSHDLFLASPNLVGSPLGLLQLVLYCIYRNKGK-------EE 240

[Close]

BLAST of Carg14030 vs. NCBI nr
Match: XP_023550598.1 (bidirectional sugar transporter SWEET3b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 268.5 bits (685), Expect = 5.1e-68
Identity = 154/271 (56.83%), Postives = 175/271 (64.58%), Query Frame = 0


Query: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60
           MGS   IRM VG+ GN ASLLLY VPILTFWRV+KKKSTEEFSCVPY+VAL+NCLLYTWY
Sbjct: 1   MGSLCPIRMAVGVIGNVASLLLYTVPILTFWRVIKKKSTEEFSCVPYMVALMNCLLYTWY 60

Query: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLV 120
            LPVVS GWENFPVVTING G+LLEFSFIF+Y  F+S + K           KKV L LV
Sbjct: 61  GLPVVSKGWENFPVVTINGLGILLEFSFIFVYFSFASSRAK-----------KKVVLGLV 120

Query: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM-------------- 180
           GV+ VF CVGMIS F L +HHLRKLFVGCIGLVASVAMY SPLVAM              
Sbjct: 121 GVLTVFFCVGMISAFVLHTHHLRKLFVGCIGLVASVAMYASPLVAMNQVLKTKSVEFMPF 180

Query: 181 ---------------------------------------LVLYCIYRNKGKEEEEEEEEE 218
                                                  LVLYCIYRNK  +++E  ++E
Sbjct: 181 YLSLFSFFASSLWLAYGLLSHDIFLASPNLVGSPLGLLQLVLYCIYRNKEHDQQEAVKKE 240

[Close]

BLAST of Carg14030 vs. ExPASy Swiss-Prot
Match: Q6NQN5 (Bidirectional sugar transporter SWEET3 OS=Arabidopsis thaliana OX=3702 GN=SWEET3 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 9.2e-44
Identity = 90/160 (56.25%), Postives = 110/160 (68.75%), Query Frame = 0


Query: 7   IRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWYALPVVS 66
           +R+ +GI GNGASLLLY  PI+TF RV KKKSTEEFSC PY++ L NCL+YTWY LP+VS
Sbjct: 5   LRLSIGILGNGASLLLYTAPIVTFSRVFKKKSTEEFSCFPYVMTLFNCLIYTWYGLPIVS 64

Query: 67  NGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLVGVVIVF 126
           + WEN P+VTING G+LLE  FIFIY +++SPK K            KV +  V V++ F
Sbjct: 65  HLWENLPLVTINGVGILLESIFIFIYFYYASPKEKI-----------KVGVTFVPVIVGF 124

Query: 127 SCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM 167
                IS      H  RK FVG +GLVAS++MYGSPLV M
Sbjct: 125 GLTTAISALVFDDHRHRKSFVGSVGLVASISMYGSPLVVM 153

[Close]

BLAST of Carg14030 vs. ExPASy Swiss-Prot
Match: Q5NAZ9 (Bidirectional sugar transporter SWEET3b OS=Oryza sativa subsp. japonica OX=39947 GN=SWEET3B PE=3 SV=2)

HSP 1 Score: 172.2 bits (435), Expect = 6.6e-42
Identity = 105/242 (43.39%), Postives = 134/242 (55.37%), Query Frame = 0


Query: 5   HSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWYALPV 64
           ++IR+ VGI GN AS+LLY  PILTF RV+KK S EEFSCVPYI+AL NCLLYTWY LPV
Sbjct: 4   NTIRVAVGILGNAASMLLYAAPILTFRRVIKKGSVEEFSCVPYILALFNCLLYTWYGLPV 63

Query: 65  VSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLVGVVI 124
           VS+GWEN  V +ING G+LLE +FI IY WF+  +RK      FVL+       ++ V+ 
Sbjct: 64  VSSGWENSTVSSINGLGILLEIAFISIYTWFAPRERK-----KFVLR------MVLPVLA 123

Query: 125 VFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVA------------------- 184
            F+   + S+F   +H LRK+FVG IGLVAS++MY SP+VA                   
Sbjct: 124 FFALTAIFSSFLFHTHGLRKVFVGSIGLVASISMYSSPMVAAKQVITTKSVEFMPFYLSL 183

Query: 185 ----------------------------------MLVLYCIYRNKGKEEEEEEEEEEEGC 194
                                              LVLYCIYR   KE E+  + ++E  
Sbjct: 184 FSFLSSALWMIYGLLGKDLFIASPNFIGCPMGILQLVLYCIYRKSHKEAEKLHDIDQENG 234

[Close]

BLAST of Carg14030 vs. ExPASy Swiss-Prot
Match: Q0DJY3 (Bidirectional sugar transporter SWEET3a OS=Oryza sativa subsp. japonica OX=39947 GN=SWEET3A PE=3 SV=2)

HSP 1 Score: 165.2 bits (417), Expect = 8.0e-40
Identity = 85/160 (53.12%), Postives = 110/160 (68.75%), Query Frame = 0


Query: 7   IRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWYALPVVS 66
           IR IVGI G+ A +LLY  PILTF RV+KK S EEFSC+PYI+AL +CL Y+WY  PVVS
Sbjct: 5   IRFIVGIIGSVACMLLYSAPILTFKRVIKKASVEEFSCIPYILALFSCLTYSWYGFPVVS 64

Query: 67  NGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLVGVVIVF 126
            GWEN  V +I+  GVL E +FI IYVWF+   +K           K+V L    ++ VF
Sbjct: 65  YGWENMTVCSISSLGVLFEGTFISIYVWFAPRGKK-----------KQVMLMASLILAVF 124

Query: 127 SCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM 167
                 S+F++ +HH+RK+FVG +GLV+S++MYGSPLVAM
Sbjct: 125 CMTVFFSSFSIHNHHIRKVFVGSVGLVSSISMYGSPLVAM 153

[Close]

BLAST of Carg14030 vs. ExPASy Swiss-Prot
Match: Q8L9J7 (Bidirectional sugar transporter SWEET1 OS=Arabidopsis thaliana OX=3702 GN=SWEET1 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.1e-23
Identity = 67/157 (42.68%), Postives = 94/157 (59.87%), Query Frame = 0


Query: 10  IVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWYALPVVSNGW 69
           I G+FGN  +L L+  P +TF R++K KSTE+FS +PY + LLNCLL  WY LP VS   
Sbjct: 7   IFGVFGNATALFLFLAPSITFKRIIKNKSTEQFSGIPYPMTLLNCLLSAWYGLPFVSK-- 66

Query: 70  ENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLVGVVIVFSCV 129
           +N  V TING G ++E  ++ I++ F +PK++ I          K+      V+ VF+ V
Sbjct: 67  DNTLVSTINGTGAVIETVYVLIFL-FYAPKKEKI----------KIFGIFSCVLAVFATV 126

Query: 130 GMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM 167
            ++S FAL+ +  RKLF G    V S+ MY SPL  M
Sbjct: 127 ALVSLFALQGNG-RKLFCGLAATVFSIIMYASPLSIM 149

[Close]

BLAST of Carg14030 vs. ExPASy Swiss-Prot
Match: B8AYH1 (Bidirectional sugar transporter SWEET1b OS=Oryza sativa subsp. indica OX=39946 GN=SWEET1B PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.1e-23
Identity = 65/163 (39.88%), Postives = 92/163 (56.44%), Query Frame = 0


Query: 8   RMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWYALPVVSN 67
           + + G+ GN  +L L+  P+ TFWR++++KSTE+FS VPY + L+NCLL  WY LP VS 
Sbjct: 6   KFLFGVSGNVIALFLFLSPVPTFWRIIRRKSTEDFSGVPYNMTLINCLLSAWYGLPFVSP 65

Query: 68  GWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLVG----VV 127
              N  V TING G ++E +++ +++ F+S              T K  LR +G    V 
Sbjct: 66  --NNILVSTINGAGAVIETAYVVVFLVFAS--------------THKTRLRTLGLAAAVA 125

Query: 128 IVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM 167
            VF+ V ++S  AL   H RKL  G    V S+ MY SPL  M
Sbjct: 126 SVFAAVALVSLLALHGQH-RKLLCGVAATVCSICMYASPLSIM 151

[Close]

BLAST of Carg14030 vs. ExPASy TrEMBL
Match: A0A6J1H294 (Bidirectional sugar transporter SWEET OS=Cucurbita moschata OX=3662 GN=LOC111459719 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 7.4e-97
Identity = 200/270 (74.07%), Postives = 201/270 (74.44%), Query Frame = 0


Query: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60
           MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY
Sbjct: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60

Query: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLV 120
           ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRK           KKVALRLV
Sbjct: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRK-----------KKVALRLV 120

Query: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM-------------- 180
           GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM              
Sbjct: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAMKQVIKTKSVEFMPF 180

Query: 181 ---------------------------------------LVLYCIYRNKGKEEEEEEEEE 218
                                                  LVLYCIYRNKGK     EEEE
Sbjct: 181 YLSFFSFSASSLWLAYGLLSHDLFLASPNLVGSPLGLLQLVLYCIYRNKGK-----EEEE 240

[Close]

BLAST of Carg14030 vs. ExPASy TrEMBL
Match: A0A6J1K3W7 (Bidirectional sugar transporter SWEET OS=Cucurbita maxima OX=3661 GN=LOC111490950 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.0e-93
Identity = 194/270 (71.85%), Postives = 196/270 (72.59%), Query Frame = 0


Query: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60
           MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY
Sbjct: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60

Query: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLV 120
           ALPVVSNGWENFPVVTINGFGVLLEFSFIFIY WFSSPKRK           K+V LRLV
Sbjct: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYFWFSSPKRK-----------KQVTLRLV 120

Query: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM-------------- 180
           GVV VFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM              
Sbjct: 121 GVVTVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAMKQVIKTKSVEYMPF 180

Query: 181 ---------------------------------------LVLYCIYRNKGKEEEEEEEEE 218
                                                  LVLYCIYRNKGK       EE
Sbjct: 181 YLSFFSFSASSLWLAYGLLSHDLFLASPNLVGSPLGLLQLVLYCIYRNKGK-------EE 240

[Close]

BLAST of Carg14030 vs. ExPASy TrEMBL
Match: A0A6J1I683 (Bidirectional sugar transporter SWEET OS=Cucurbita maxima OX=3661 GN=LOC111471487 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 8.0e-67
Identity = 155/271 (57.20%), Postives = 172/271 (63.47%), Query Frame = 0


Query: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60
           MGS   IRM VG+ GN ASLLLY VPILTFWRV+KKKSTEEFSCVPY+VAL+NCLLYTWY
Sbjct: 1   MGSLCPIRMAVGVIGNAASLLLYTVPILTFWRVIKKKSTEEFSCVPYMVALMNCLLYTWY 60

Query: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLV 120
            LPVVS GWENFPVVTING G+LLEFSFIFIY  F+S + K           KK  L LV
Sbjct: 61  GLPVVSKGWENFPVVTINGLGILLEFSFIFIYFSFASSRPK-----------KKAVLGLV 120

Query: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM-------------- 180
           GV+ VF CVGMIS F L +HHLRKLFVGCIGLVASVAMY +PLVAM              
Sbjct: 121 GVLTVFFCVGMISAFVLHTHHLRKLFVGCIGLVASVAMYAAPLVAMNQVIKTKSVKFMPF 180

Query: 181 ---------------------------------------LVLYCIYRNKGKEEEEEEEEE 218
                                                  LVLYCIYRN  KE ++E  +E
Sbjct: 181 YLSLFSFFASSMWLAYGLLSHDIFLASPNLVGSPLGLLQLVLYCIYRN--KEHQQEAVKE 240

[Close]

BLAST of Carg14030 vs. ExPASy TrEMBL
Match: A0A6J1E3F0 (Bidirectional sugar transporter SWEET OS=Cucurbita moschata OX=3662 GN=LOC111430243 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 8.0e-67
Identity = 153/271 (56.46%), Postives = 173/271 (63.84%), Query Frame = 0


Query: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60
           MGS   IRM VG+ GN ASLLLY  PILTFWRV+KKKSTEEFSCVPY+VAL+NCLLYTWY
Sbjct: 1   MGSVCPIRMAVGVIGNAASLLLYTAPILTFWRVIKKKSTEEFSCVPYMVALMNCLLYTWY 60

Query: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLV 120
            LPVVS GWENFPVVTING G+LLEFSFIF+Y  F+S + K           KKV L LV
Sbjct: 61  GLPVVSKGWENFPVVTINGLGILLEFSFIFVYFSFASSRAK-----------KKVVLGLV 120

Query: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM-------------- 180
           GV+ VF CVGMIS F L +HHLRKLFVGCIGLVASVAMY SPLVAM              
Sbjct: 121 GVLTVFFCVGMISAFVLHTHHLRKLFVGCIGLVASVAMYASPLVAMNQVIKTKSVEFMPF 180

Query: 181 ---------------------------------------LVLYCIYRNKGKEEEEEEEEE 218
                                                  LVLYCIYRNK + + E  ++E
Sbjct: 181 YLSLFSFFASSLWLAYGLLSHDIFLASPNLVGSPLGLLQLVLYCIYRNK-EHQREAVKKE 240

[Close]

BLAST of Carg14030 vs. ExPASy TrEMBL
Match: A0A0A0KNA4 (Bidirectional sugar transporter SWEET OS=Cucumis sativus OX=3659 GN=Csa_5G139600 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 4.4e-65
Identity = 148/271 (54.61%), Postives = 171/271 (63.10%), Query Frame = 0


Query: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60
           M S ++IRM VGI GNGASLLLY VPILTFWRV+KKKSTEEFSCVPYIVAL+NCLLYTWY
Sbjct: 1   MRSLYTIRMAVGIIGNGASLLLYTVPILTFWRVIKKKSTEEFSCVPYIVALMNCLLYTWY 60

Query: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLV 120
            LP+VS GWENFPVVTING G+LLE SFI IY  F+S + K           KKV L++V
Sbjct: 61  GLPIVSKGWENFPVVTINGLGILLELSFISIYFCFASSQAK-----------KKVVLKMV 120

Query: 121 GVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM-------------- 180
           GVV VF CVGMIS+F LK+HHLRK FVGCIGLVAS+AMY SPLVAM              
Sbjct: 121 GVVTVFLCVGMISSFVLKTHHLRKFFVGCIGLVASIAMYASPLVAMKQVIKTKSVEFMPF 180

Query: 181 ---------------------------------------LVLYCIYRNKGKEEEEEEEEE 218
                                                  LVLYCIYRNK + E+   ++E
Sbjct: 181 YLSFFSFSASSLWLAYGLLSHDLFLASPNLVGSPLGLLQLVLYCIYRNK-EHEQGVLKKE 240

[Close]

BLAST of Carg14030 vs. TAIR 10
Match: AT5G53190.1 (Nodulin MtN3 family protein )

HSP 1 Score: 178.3 bits (451), Expect = 6.5e-45
Identity = 90/160 (56.25%), Postives = 110/160 (68.75%), Query Frame = 0


Query: 7   IRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWYALPVVS 66
           +R+ +GI GNGASLLLY  PI+TF RV KKKSTEEFSC PY++ L NCL+YTWY LP+VS
Sbjct: 5   LRLSIGILGNGASLLLYTAPIVTFSRVFKKKSTEEFSCFPYVMTLFNCLIYTWYGLPIVS 64

Query: 67  NGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLVGVVIVF 126
           + WEN P+VTING G+LLE  FIFIY +++SPK K            KV +  V V++ F
Sbjct: 65  HLWENLPLVTINGVGILLESIFIFIYFYYASPKEKI-----------KVGVTFVPVIVGF 124

Query: 127 SCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM 167
                IS      H  RK FVG +GLVAS++MYGSPLV M
Sbjct: 125 GLTTAISALVFDDHRHRKSFVGSVGLVASISMYGSPLVVM 153

[Close]

BLAST of Carg14030 vs. TAIR 10
Match: AT1G21460.1 (Nodulin MtN3 family protein )

HSP 1 Score: 110.2 bits (274), Expect = 2.2e-24
Identity = 67/157 (42.68%), Postives = 94/157 (59.87%), Query Frame = 0


Query: 10  IVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWYALPVVSNGW 69
           I G+FGN  +L L+  P +TF R++K KSTE+FS +PY + LLNCLL  WY LP VS   
Sbjct: 7   IFGVFGNATALFLFLAPSITFKRIIKNKSTEQFSGIPYPMTLLNCLLSAWYGLPFVSK-- 66

Query: 70  ENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLVGVVIVFSCV 129
           +N  V TING G ++E  ++ I++ F +PK++ I          K+      V+ VF+ V
Sbjct: 67  DNTLVSTINGTGAVIETVYVLIFL-FYAPKKEKI----------KIFGIFSCVLAVFATV 126

Query: 130 GMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM 167
            ++S FAL+ +  RKLF G    V S+ MY SPL  M
Sbjct: 127 ALVSLFALQGNG-RKLFCGLAATVFSIIMYASPLSIM 149

[Close]

BLAST of Carg14030 vs. TAIR 10
Match: AT5G40260.1 (Nodulin MtN3 family protein )

HSP 1 Score: 108.2 bits (269), Expect = 8.3e-24
Identity = 59/161 (36.65%), Postives = 96/161 (59.63%), Query Frame = 0


Query: 7   IRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWYALPVVS 66
           +R I+G+ GN  S  L+  P  TFWR+ KKKS EEFS VPY+  ++NC+L+ +Y LPVV 
Sbjct: 7   VRFIIGVIGNVISFGLFAAPAKTFWRIFKKKSVEEFSYVPYVATVMNCMLWVFYGLPVVH 66

Query: 67  NGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLVGVVIVF 126
              ++  V TING G+++E  ++ +Y+ +   K+            + +   L   VI+ 
Sbjct: 67  K--DSILVSTINGVGLVIELFYVGVYLMYCGHKKN---------HRRNILGFLALEVILV 126

Query: 127 SCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAML 168
             + +I+ FALK   +++ FVG I  V ++AMYG+P +A++
Sbjct: 127 VAIILITLFALKGDFVKQTFVGVICDVFNIAMYGAPSLAII 156

[Close]

BLAST of Carg14030 vs. TAIR 10
Match: AT5G40260.2 (Nodulin MtN3 family protein )

HSP 1 Score: 108.2 bits (269), Expect = 8.3e-24
Identity = 59/161 (36.65%), Postives = 96/161 (59.63%), Query Frame = 0


Query: 7   IRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWYALPVVS 66
           +R I+G+ GN  S  L+  P  TFWR+ KKKS EEFS VPY+  ++NC+L+ +Y LPVV 
Sbjct: 7   VRFIIGVIGNVISFGLFAAPAKTFWRIFKKKSVEEFSYVPYVATVMNCMLWVFYGLPVVH 66

Query: 67  NGWENFPVVTINGFGVLLEFSFIFIYVWFSSPKRKAITRSSFVLKTKKVALRLVGVVIVF 126
              ++  V TING G+++E  ++ +Y+ +   K+            + +   L   VI+ 
Sbjct: 67  K--DSILVSTINGVGLVIELFYVGVYLMYCGHKKN---------HRRNILGFLALEVILV 126

Query: 127 SCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAML 168
             + +I+ FALK   +++ FVG I  V ++AMYG+P +A++
Sbjct: 127 VAIILITLFALKGDFVKQTFVGVICDVFNIAMYGAPSLAII 156

[Close]

BLAST of Carg14030 vs. TAIR 10
Match: AT5G62850.1 (Nodulin MtN3 family protein )

HSP 1 Score: 92.0 bits (227), Expect = 6.1e-19
Identity = 57/167 (34.13%), Postives = 91/167 (54.49%), Query Frame = 0


Query: 1   MGSHHSIRMIVGIFGNGASLLLYCVPILTFWRVVKKKSTEEFSCVPYIVALLNCLLYTWY 60
           M   H+ R IVGI GN  S  L+C PI T  ++ K KS  EF   PY+  +LNC+++T+Y
Sbjct: 1   MTDPHTARTIVGIVGNVISFGLFCAPIPTMVKIWKMKSVSEFKPDPYVATVLNCMMWTFY 60

Query: 61  ALPVVSNGWENFPVVTINGFGVLLEFSFIFI-YVWFSSPKRKAITRSSFVLKTKKVALRL 120
            LP V    ++  V+TING G+ +E  ++ I +V+ +SP R            +K+ + +
Sbjct: 61  GLPFVQP--DSLLVITINGTGLFMELVYVTIFFVFATSPVR------------RKITIAM 120

Query: 121 VGVVIVFSCVGMISTFALKSHHLRKLFVGCIGLVASVAMYGSPLVAM 167
           V  VI  + V   + + L +   R + +G + +V +V MY +PL  M
Sbjct: 121 VIEVIFMAVVIFCTMYFLHTTKQRSMLIGILCIVFNVIMYAAPLTVM 153

The following BLAST results are available for this feature:

BLAST of Carg14030 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035735.1	7.8e-117	100.00	Bidirectional sugar transporter SWEET3b, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022958511.1	1.5e-96	74.07	bidirectional sugar transporter SWEET3b-like [Cucurbita moschata]	[more]
XP_023534737.1	5.8e-96	73.70	bidirectional sugar transporter SWEET3b-like [Cucurbita pepo subsp. pepo]	[more]
XP_022995390.1	2.1e-93	71.85	bidirectional sugar transporter SWEET3b-like [Cucurbita maxima]	[more]
XP_023550598.1	5.1e-68	56.83	bidirectional sugar transporter SWEET3b-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg14030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NQN5	9.2e-44	56.25	Bidirectional sugar transporter SWEET3 OS=Arabidopsis thaliana OX=3702 GN=SWEET3...	[more]
Q5NAZ9	6.6e-42	43.39	Bidirectional sugar transporter SWEET3b OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q0DJY3	8.0e-40	53.13	Bidirectional sugar transporter SWEET3a OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q8L9J7	3.1e-23	42.68	Bidirectional sugar transporter SWEET1 OS=Arabidopsis thaliana OX=3702 GN=SWEET1...	[more]
B8AYH1	3.1e-23	39.88	Bidirectional sugar transporter SWEET1b OS=Oryza sativa subsp. indica OX=39946 G...	[more]

BLAST of Carg14030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H294	7.4e-97	74.07	Bidirectional sugar transporter SWEET OS=Cucurbita moschata OX=3662 GN=LOC111459...	[more]
A0A6J1K3W7	1.0e-93	71.85	Bidirectional sugar transporter SWEET OS=Cucurbita maxima OX=3661 GN=LOC11149095...	[more]
A0A6J1I683	8.0e-67	57.20	Bidirectional sugar transporter SWEET OS=Cucurbita maxima OX=3661 GN=LOC11147148...	[more]
A0A6J1E3F0	8.0e-67	56.46	Bidirectional sugar transporter SWEET OS=Cucurbita moschata OX=3662 GN=LOC111430...	[more]
A0A0A0KNA4	4.4e-65	54.61	Bidirectional sugar transporter SWEET OS=Cucumis sativus OX=3659 GN=Csa_5G139600...	[more]

BLAST of Carg14030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53190.1	6.5e-45	56.25	Nodulin MtN3 family protein	[more]
AT1G21460.1	2.2e-24	42.68	Nodulin MtN3 family protein	[more]
AT5G40260.1	8.3e-24	36.65	Nodulin MtN3 family protein	[more]
AT5G40260.2	8.3e-24	36.65	Nodulin MtN3 family protein	[more]
AT5G62850.1	6.1e-19	34.13	Nodulin MtN3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004316	SWEET sugar transporter	PFAM	PF03083	MtN3_slv	coord: 10..99 e-value: 3.7E-15 score: 55.7
None	No IPR available	GENE3D	1.20.1280.290		coord: 12..108 e-value: 5.7E-17 score: 63.7
None	No IPR available	PANTHER	PTHR10791:SF28	BIDIRECTIONAL SUGAR TRANSPORTER SWEET3	coord: 7..166
None	No IPR available	PANTHER	PTHR10791	RAG1-ACTIVATING PROTEIN 1	coord: 7..166
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 45..187

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg14030-RA	Carg14030-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008643	carbohydrate transport
cellular_component	GO:0016021	integral component of membrane