Carg13407 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg13407
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Autophagy-related protein
Location	Carg_Chr08: 2605431 .. 2607814 (-)
RNA-Seq Expression	Carg13407
Synteny	Carg13407

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGAGCACTGATGGGTAAAGCCAAATCCTTCAAGAACGAGTTCTCCTATGGTATGAACCTTTTTATGGCTTAAAATTCAAGATGATTTTCGTTGTTTTTCATCAATTTGTCTCTTAGTTTGATACGTTTGGAGATCCTTATTGAATCATGGCGTTGAATTTTTCGTAGAACAACGATCGGAGGAGTCGTTCGAAATCTTGATCAAGTATCCGGATCGAGTCCCCGTAAGTTGCCAAATTCTCACATTATGCGTTTCTTATTTGAGAAGATTCGTATTGTTGCATTTTGTTTAGAAATTATAGATGTTGGTGAATTTATGAATTTGGTGGAAATAGGTAACTCAGAAAGTCTGCAGGTGTCCAATCCTTAGGCGTTTATCTTTGTTTCCTCTTGGTTGAAGCGTTATCATTATGATTTTTTGTTTTTTTTCTTAGAGATTGGTCGCACAATTTGCTATGAGGGATGCTTTTCAGATAAATTCTTCAGGAAATGAAATGCTGAAGTTATCAGATGATTTTAGTCAATGTTTTCTTTGTTATCTAAGAACAAAATTGTATCCGGATTTTTGTTCAAATTGTGGATAGAAGCTGCTTGCTGTTTGTATCAAGCGATGCCAAGGCGATTTCAAGTTTGGTAGGCTTGGACAGAGCTATATAGAAGGAAGCAAATCACTATTTTTGTTTCCTAAAAAACAATATCACTGAAGTTAATGTGCAGACTCATTTATGTGATGTTCTTATATTTTTGAGGTGTAAATGCTAGATTGCTGTTCTTCATTTCCTCCAAATCCATGAAAGTGATTTAATGGTTTTCCTTTTGATGTTCTTTCTGAGTATAATTGCAGGTAATTGTCGAGAGATATGAGAAATGTGAACTTCCTGAGATGGAAAAGAAGAAGTAAGTTCTTCCTGCATATCTAAGCTTCCTCAATGTTTCAGTTCTCCTAATTTCATGTACCTCTCTCTGAAAATCAATTGAATGGAAACAGACACTAGCACCTTTGCATTACACTCTTAACATGTTTCTGTTTGTTCTTCTTAATGTTTGAGAAGCCATTTTTGGGTACTGGCTAACGATCGTAAGCGTCGAAATCATCGAATTTCAATGTTCCTCACAAAATTGTCGAAAAATTATCGATTGTGACTCAGTTTTTTTTCAAATGGCCCAATCAAAGTTTCCAAATTAGGGTCTCGGTTTTAATCTTGTACGTATGGTGCTAGACTCTCTTTCTTCGAGATTGTTAATGTTTGTTCTTGTCTGTCTTATTTTTCTGGCTAGCTATCACATGATCATCCTCCCCAAATAAAAAACATCATGGTATTTATTTATTCACATTTGGAAATTAAAACATAGTCTCACATAATGTCTTACACGTTGTATTCCAACATTTTCTCGTCTCGTAAGACACGATTTTCATTGAATTTCTAAATTAGGGTCTCGGTTTTAACCCTGTACGTATGGTGCTATAGGCTCTCTTTTCTTCGAGATTGTTAATGTTTGTTCTTGTCTGTCTTATTTTTCTGGCTATCTATCAATTGATCATCCTCTCCCGAAAAAAAAACATCATGGTATTTATTTATTCACATTTGGGAATTAAAACATAGTTTCACATAATGTCTTACACGTTGTATTCCAACATTTTCTCGTCTTGTTAGATACGATTTTCATTGAATTTCTGCAGAACTATGGGATCTGAACAGGGGTTTTGTACATGTTGCCTAGGTTTACATTTGAAAGCCGGTTGTGATCCTAAAATAACATGACATATTGTGTCAATTAGTTTTGGTTTGTCTGTTGAATGTCTTTTGTTTTGTTATTGACTAAAGGTACCTGATTCCTCGAGACATGTCTGTTGGCCAATTCATCCATGTCCTGAGCACCAGGATTCGTCTAGCACCTGGAAAAGCTCTCTTTGTATTTGTAAAGAATACTTTGCCTCAAACAGGTAGGGTTAGGACATAGGATTACATTTGACATGATGCTGCTGCCCATTTTTACGTGCATTCTCGTGCAACAAGTCGTTTTAATCCTAAACAGTTGCCCTCAAATTTTGCAGCTAGCTTAATGAGTACCGTGTACGAATCATACAAGGACGACGATGGCTTTCTGTACATGTGTTACAGCAGTGAAAAGACCTTTGGCTAAGGTAATAATCTGATTGAGATTTGTGAATGCTGAAGTTGTTGTATACTTTCTGCTGCTCCTCCTGTTGTGAGTATTATATTCCATGAACAAATTTGTTCTTACCATGTAGACATTATTATGTATAATGTGTAAATCATTTAATATTGTAACGGCCTAAGCCTACTGCTAGTAGATATTGTCCTCTTTGGCCTTTCTCTTTCGGACTTCCCCTCAAGGTTTTTAAAACGCGTCTGCTAGGA

mRNA sequence

GAGAGCACTGATGGGTAAAGCCAAATCCTTCAAGAACGAGTTCTCCTATGAACAACGATCGGAGGAGTCGTTCGAAATCTTGATCAAGTATCCGGATCGAGTCCCCGTAATTGTCGAGAGATATGAGAAATGTGAACTTCCTGAGATGGAAAAGAAGAAGTACCTGATTCCTCGAGACATGTCTGTTGGCCAATTCATCCATGTCCTGAGCACCAGGATTCGTCTAGCACCTGGAAAAGCTCTCTTTGTATTTGTAAAGAATACTTTGCCTCAAACAGCTAGCTTAATGAGTACCGTGTACGAATCATACAAGGACGACGATGGCTTTCTGTACATGTGTTACAGCAGTGAAAAGACCTTTGGCTAAGGTAATAATCTGATTGAGATTTGTGAATGCTGAAGTTGTTGTATACTTTCTGCTGCTCCTCCTGTTGTGAGTATTATATTCCATGAACAAATTTGTTCTTACCATGTAGACATTATTATGTATAATGTGTAAATCATTTAATATTGTAACGGCCTAAGCCTACTGCTAGTAGATATTGTCCTCTTTGGCCTTTCTCTTTCGGACTTCCCCTCAAGGTTTTTAAAACGCGTCTGCTAGGA

Coding sequence (CDS)

ATGGGTAAAGCCAAATCCTTCAAGAACGAGTTCTCCTATGAACAACGATCGGAGGAGTCGTTCGAAATCTTGATCAAGTATCCGGATCGAGTCCCCGTAATTGTCGAGAGATATGAGAAATGTGAACTTCCTGAGATGGAAAAGAAGAAGTACCTGATTCCTCGAGACATGTCTGTTGGCCAATTCATCCATGTCCTGAGCACCAGGATTCGTCTAGCACCTGGAAAAGCTCTCTTTGTATTTGTAAAGAATACTTTGCCTCAAACAGCTAGCTTAATGAGTACCGTGTACGAATCATACAAGGACGACGATGGCTTTCTGTACATGTGTTACAGCAGTGAAAAGACCTTTGGCTAA

Protein sequence

MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVGQFIHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG

Homology

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BLAST of Carg13407 vs. NCBI nr
Match: KAG7025617.1 (Autophagy-related protein 8i [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 237.3 bits (604), Expect = 6.9e-59
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60
           MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG
Sbjct: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60

Query: 61  QFIHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           QFIHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG
Sbjct: 61  QFIHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 118

[Close]

BLAST of Carg13407 vs. NCBI nr
Match: KAG6593267.1 (Autophagy-related protein 8i, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 236.5 bits (602), Expect = 1.2e-58
Identity = 117/118 (99.15%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60
           MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG
Sbjct: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60

Query: 61  QFIHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           QFIHVLSTR+RLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG
Sbjct: 61  QFIHVLSTRLRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 118

[Close]

BLAST of Carg13407 vs. NCBI nr
Match: XP_022960552.1 (autophagy-related protein 8i-like [Cucurbita moschata])

HSP 1 Score: 235.0 bits (598), Expect = 3.4e-58
Identity = 116/118 (98.31%), Postives = 117/118 (99.15%), Query Frame = 0


Query: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60
           MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG
Sbjct: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60

Query: 61  QFIHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           QFIHVLSTR+RLAPGKALFVFVKNTLPQTASLMST YESYKDDDGFLYMCYSSEKTFG
Sbjct: 61  QFIHVLSTRLRLAPGKALFVFVKNTLPQTASLMSTAYESYKDDDGFLYMCYSSEKTFG 118

[Close]

BLAST of Carg13407 vs. NCBI nr
Match: XP_023004698.1 (autophagy-related protein 8i-like [Cucurbita maxima])

HSP 1 Score: 232.6 bits (592), Expect = 1.7e-57
Identity = 115/118 (97.46%), Postives = 116/118 (98.31%), Query Frame = 0


Query: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60
           MGKAKSFKNEFSYEQR EESFEILIKYPDRVPVIVERYEKCE PEMEKKKYLIPRDMSVG
Sbjct: 1   MGKAKSFKNEFSYEQRLEESFEILIKYPDRVPVIVERYEKCEFPEMEKKKYLIPRDMSVG 60

Query: 61  QFIHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           QFIHVLSTR+RLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG
Sbjct: 61  QFIHVLSTRLRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 118

[Close]

BLAST of Carg13407 vs. NCBI nr
Match: XP_023513717.1 (autophagy-related protein 8i-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 232.3 bits (591), Expect = 2.2e-57
Identity = 115/118 (97.46%), Postives = 116/118 (98.31%), Query Frame = 0


Query: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60
           MGKAKSFKNEFSY QRSEESFEILIKYPDRVPVIVERYEKCE PEMEKKKYLIPRDMSVG
Sbjct: 1   MGKAKSFKNEFSYVQRSEESFEILIKYPDRVPVIVERYEKCEFPEMEKKKYLIPRDMSVG 60

Query: 61  QFIHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           QFIHVLSTR+RLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG
Sbjct: 61  QFIHVLSTRLRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 118

[Close]

BLAST of Carg13407 vs. ExPASy Swiss-Prot
Match: Q9LRP7 (Autophagy-related protein 8i OS=Arabidopsis thaliana OX=3702 GN=ATG8I PE=1 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 4.7e-42
Identity = 80/114 (70.18%), Postives = 99/114 (86.84%), Query Frame = 0


Query: 5   KSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVGQFIH 64
           KSFK +++ ++R  ES EI+ KYP R+PVI E+Y K +LP +EKKK+L+PRDMSVGQFI+
Sbjct: 2   KSFKEQYTLDERLAESREIIAKYPTRIPVIAEKYCKTDLPAIEKKKFLVPRDMSVGQFIY 61

Query: 65  VLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           +LS R+ L+PGKALFVFV NTLPQTA+LM +VYESYKDDDGF+YMCYSSEKTFG
Sbjct: 62  ILSARLHLSPGKALFVFVNNTLPQTAALMDSVYESYKDDDGFVYMCYSSEKTFG 115

[Close]

BLAST of Carg13407 vs. ExPASy Swiss-Prot
Match: Q8S925 (Autophagy-related protein 8h OS=Arabidopsis thaliana OX=3702 GN=ATG8H PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 1.7e-39
Identity = 74/114 (64.91%), Postives = 97/114 (85.09%), Query Frame = 0


Query: 5   KSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVGQFIH 64
           KSFK++FS ++R +ES  I+ KYPDR+PVI+E+Y   +LP+MEK KYL+PRDM+VG FIH
Sbjct: 6   KSFKDQFSSDERLKESNNIIAKYPDRIPVIIEKYSNADLPDMEKNKYLVPRDMTVGHFIH 65

Query: 65  VLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           +LS R++L P KALFVFV NTLPQTAS M ++Y ++K++DGFLYMCYS+EKTFG
Sbjct: 66  MLSKRMQLDPSKALFVFVHNTLPQTASRMDSLYNTFKEEDGFLYMCYSTEKTFG 119

[Close]

BLAST of Carg13407 vs. ExPASy Swiss-Prot
Match: Q2RBS4 (Autophagy-related protein 8D OS=Oryza sativa subsp. japonica OX=39947 GN=ATG8D PE=3 SV=2)

HSP 1 Score: 158.3 bits (399), Expect = 5.3e-38
Identity = 75/117 (64.10%), Postives = 96/117 (82.05%), Query Frame = 0


Query: 3   KAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVGQF 62
           K + FK EF+ E+R++ES  ++  YPDR+PVIVE++ +  LPEMEK+KYL+P +M VGQF
Sbjct: 2   KPRPFKEEFTLEERAKESAAMIASYPDRIPVIVEKFSRSNLPEMEKRKYLVPCNMPVGQF 61

Query: 63  IHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDD-DGFLYMCYSSEKTFG 119
           I +L +R+ L+PG ALFVFV NTLPQTA LM +VYESYKD+ DGFLY+CYSSEKTFG
Sbjct: 62  IFILRSRLHLSPGTALFVFVNNTLPQTAQLMGSVYESYKDEGDGFLYLCYSSEKTFG 118

[Close]

BLAST of Carg13407 vs. ExPASy Swiss-Prot
Match: Q9SL04 (Autophagy-related protein 8d OS=Arabidopsis thaliana OX=3702 GN=ATG8D PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 3.6e-34
Identity = 66/113 (58.41%), Postives = 91/113 (80.53%), Query Frame = 0


Query: 6   SFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVGQFIHV 65
           SFK+E   E+R  E+  I  KYPDR+PVIVER EK ++P++++KKYL+P D++VGQF++V
Sbjct: 5   SFKHEHPLEKRQAEAARIREKYPDRIPVIVERAEKSDVPDIDRKKYLVPADLTVGQFVYV 64

Query: 66  LSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           +  RI+L+P KA+F+FVKN LP TA++MS +YE +KD+DGFLYM YS E TFG
Sbjct: 65  VRKRIKLSPEKAIFIFVKNILPPTAAIMSAIYEEHKDEDGFLYMSYSGENTFG 117

[Close]

BLAST of Carg13407 vs. ExPASy Swiss-Prot
Match: Q2XPP5 (Autophagy-related protein 8A OS=Oryza sativa subsp. indica OX=39946 GN=ATG8A PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 4.7e-34
Identity = 70/113 (61.95%), Postives = 89/113 (78.76%), Query Frame = 0


Query: 6   SFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVGQFIHV 65
           SFK E   E+R  ES  I  KY DR+PVIVE+ +K ++PE++KKKYL+P D++VGQF++V
Sbjct: 5   SFKLEHPLERRQAESARIREKYSDRIPVIVEKADKTDVPEIDKKKYLVPADLTVGQFVYV 64

Query: 66  LSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           +  RI+L+P KA+FVFVKNTLP TASLMS +YE  KD+DGFLYM YS E TFG
Sbjct: 65  VRKRIKLSPEKAIFVFVKNTLPPTASLMSAIYEENKDEDGFLYMTYSGENTFG 117

[Close]

BLAST of Carg13407 vs. ExPASy TrEMBL
Match: A0A6J1H7R2 (Autophagy-related protein OS=Cucurbita moschata OX=3662 GN=LOC111461254 PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.7e-58
Identity = 116/118 (98.31%), Postives = 117/118 (99.15%), Query Frame = 0


Query: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60
           MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG
Sbjct: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60

Query: 61  QFIHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           QFIHVLSTR+RLAPGKALFVFVKNTLPQTASLMST YESYKDDDGFLYMCYSSEKTFG
Sbjct: 61  QFIHVLSTRLRLAPGKALFVFVKNTLPQTASLMSTAYESYKDDDGFLYMCYSSEKTFG 118

[Close]

BLAST of Carg13407 vs. ExPASy TrEMBL
Match: A0A6J1L0B7 (Autophagy-related protein OS=Cucurbita maxima OX=3661 GN=LOC111497916 PE=3 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 8.2e-58
Identity = 115/118 (97.46%), Postives = 116/118 (98.31%), Query Frame = 0


Query: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60
           MGKAKSFKNEFSYEQR EESFEILIKYPDRVPVIVERYEKCE PEMEKKKYLIPRDMSVG
Sbjct: 1   MGKAKSFKNEFSYEQRLEESFEILIKYPDRVPVIVERYEKCEFPEMEKKKYLIPRDMSVG 60

Query: 61  QFIHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           QFIHVLSTR+RLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG
Sbjct: 61  QFIHVLSTRLRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 118

[Close]

BLAST of Carg13407 vs. ExPASy TrEMBL
Match: A0A6J1GQC4 (Autophagy-related protein OS=Cucurbita moschata OX=3662 GN=LOC111456510 PE=3 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.5e-54
Identity = 109/118 (92.37%), Postives = 112/118 (94.92%), Query Frame = 0


Query: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60
           MGK KSFK+EF+YEQR  ESFEIL KYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG
Sbjct: 1   MGKTKSFKDEFTYEQRFAESFEILAKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60

Query: 61  QFIHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           QFIHVLSTR+ LAPGKALFVFVKNTLPQTASLM TVYESYKDDDGFLYMCYSSEKTFG
Sbjct: 61  QFIHVLSTRLHLAPGKALFVFVKNTLPQTASLMDTVYESYKDDDGFLYMCYSSEKTFG 118

[Close]

BLAST of Carg13407 vs. ExPASy TrEMBL
Match: A0A6J1CW54 (Autophagy-related protein OS=Momordica charantia OX=3673 GN=LOC111014838 PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.5e-54
Identity = 107/118 (90.68%), Postives = 114/118 (96.61%), Query Frame = 0


Query: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60
           MGK+KSFKNEF+YEQR EE+FEIL KYPDRVPVIVERY+KCELPEMEKKKYLIPRDMSVG
Sbjct: 1   MGKSKSFKNEFTYEQRFEEAFEILAKYPDRVPVIVERYDKCELPEMEKKKYLIPRDMSVG 60

Query: 61  QFIHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           QFIHVLS R+ LAPGKALFVFVKNTLPQTASLMS+VYESYKDDDGFLYMCYS+EKTFG
Sbjct: 61  QFIHVLSNRLHLAPGKALFVFVKNTLPQTASLMSSVYESYKDDDGFLYMCYSTEKTFG 118

[Close]

BLAST of Carg13407 vs. ExPASy TrEMBL
Match: A0A5D3CGI6 (Autophagy-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00040 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 4.2e-54
Identity = 107/118 (90.68%), Postives = 113/118 (95.76%), Query Frame = 0


Query: 1   MGKAKSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVG 60
           MG+ KSFK+EF+YEQR EESFE+L KYPDRVPVIVERY KCELPEMEKKKYLIPRDMSVG
Sbjct: 1   MGRTKSFKDEFTYEQRFEESFEVLAKYPDRVPVIVERYGKCELPEMEKKKYLIPRDMSVG 60

Query: 61  QFIHVLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           QFIHVLSTR+RLAPGKALFVFV NTLPQTASLMSTVY+SYKDDDGFLYMCYSSEKTFG
Sbjct: 61  QFIHVLSTRLRLAPGKALFVFVNNTLPQTASLMSTVYDSYKDDDGFLYMCYSSEKTFG 118

[Close]

BLAST of Carg13407 vs. TAIR 10
Match: AT3G15580.1 (Ubiquitin-like superfamily protein )

HSP 1 Score: 171.8 bits (434), Expect = 3.3e-43
Identity = 80/114 (70.18%), Postives = 99/114 (86.84%), Query Frame = 0


Query: 5   KSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVGQFIH 64
           KSFK +++ ++R  ES EI+ KYP R+PVI E+Y K +LP +EKKK+L+PRDMSVGQFI+
Sbjct: 2   KSFKEQYTLDERLAESREIIAKYPTRIPVIAEKYCKTDLPAIEKKKFLVPRDMSVGQFIY 61

Query: 65  VLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           +LS R+ L+PGKALFVFV NTLPQTA+LM +VYESYKDDDGF+YMCYSSEKTFG
Sbjct: 62  ILSARLHLSPGKALFVFVNNTLPQTAALMDSVYESYKDDDGFVYMCYSSEKTFG 115

[Close]

BLAST of Carg13407 vs. TAIR 10
Match: AT3G06420.1 (Ubiquitin-like superfamily protein )

HSP 1 Score: 163.3 bits (412), Expect = 1.2e-40
Identity = 74/114 (64.91%), Postives = 97/114 (85.09%), Query Frame = 0


Query: 5   KSFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVGQFIH 64
           KSFK++FS ++R +ES  I+ KYPDR+PVI+E+Y   +LP+MEK KYL+PRDM+VG FIH
Sbjct: 6   KSFKDQFSSDERLKESNNIIAKYPDRIPVIIEKYSNADLPDMEKNKYLVPRDMTVGHFIH 65

Query: 65  VLSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           +LS R++L P KALFVFV NTLPQTAS M ++Y ++K++DGFLYMCYS+EKTFG
Sbjct: 66  MLSKRMQLDPSKALFVFVHNTLPQTASRMDSLYNTFKEEDGFLYMCYSTEKTFG 119

[Close]

BLAST of Carg13407 vs. TAIR 10
Match: AT2G05630.1 (Ubiquitin-like superfamily protein )

HSP 1 Score: 145.6 bits (366), Expect = 2.5e-35
Identity = 66/113 (58.41%), Postives = 91/113 (80.53%), Query Frame = 0


Query: 6   SFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVGQFIHV 65
           SFK+E   E+R  E+  I  KYPDR+PVIVER EK ++P++++KKYL+P D++VGQF++V
Sbjct: 5   SFKHEHPLEKRQAEAARIREKYPDRIPVIVERAEKSDVPDIDRKKYLVPADLTVGQFVYV 64

Query: 66  LSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           +  RI+L+P KA+F+FVKN LP TA++MS +YE +KD+DGFLYM YS E TFG
Sbjct: 65  VRKRIKLSPEKAIFIFVKNILPPTAAIMSAIYEEHKDEDGFLYMSYSGENTFG 117

[Close]

BLAST of Carg13407 vs. TAIR 10
Match: AT1G62040.1 (Ubiquitin-like superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 8.2e-34
Identity = 67/113 (59.29%), Postives = 88/113 (77.88%), Query Frame = 0


Query: 6   SFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVGQFIHV 65
           SFK E   E+R  ES  I  KYPDR+PVIVER E+ ++P ++KKKYL+P D++VGQF++V
Sbjct: 5   SFKLEHPLERRQIESSRIREKYPDRIPVIVERAERSDVPNIDKKKYLVPADLTVGQFVYV 64

Query: 66  LSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           +  RI+L+  KA+FVFVKNTLP TA++MS +Y+  KD+DGFLYM YS E TFG
Sbjct: 65  VRKRIKLSAEKAIFVFVKNTLPPTAAMMSAIYDENKDEDGFLYMTYSGENTFG 117

[Close]

BLAST of Carg13407 vs. TAIR 10
Match: AT1G62040.2 (Ubiquitin-like superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 8.2e-34
Identity = 67/113 (59.29%), Postives = 88/113 (77.88%), Query Frame = 0


Query: 6   SFKNEFSYEQRSEESFEILIKYPDRVPVIVERYEKCELPEMEKKKYLIPRDMSVGQFIHV 65
           SFK E   E+R  ES  I  KYPDR+PVIVER E+ ++P ++KKKYL+P D++VGQF++V
Sbjct: 19  SFKLEHPLERRQIESSRIREKYPDRIPVIVERAERSDVPNIDKKKYLVPADLTVGQFVYV 78

Query: 66  LSTRIRLAPGKALFVFVKNTLPQTASLMSTVYESYKDDDGFLYMCYSSEKTFG 119
           +  RI+L+  KA+FVFVKNTLP TA++MS +Y+  KD+DGFLYM YS E TFG
Sbjct: 79  VRKRIKLSAEKAIFVFVKNTLPPTAAMMSAIYDENKDEDGFLYMTYSGENTFG 131

The following BLAST results are available for this feature:

BLAST of Carg13407 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025617.1	6.9e-59	100.00	Autophagy-related protein 8i [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6593267.1	1.2e-58	99.15	Autophagy-related protein 8i, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022960552.1	3.4e-58	98.31	autophagy-related protein 8i-like [Cucurbita moschata]	[more]
XP_023004698.1	1.7e-57	97.46	autophagy-related protein 8i-like [Cucurbita maxima]	[more]
XP_023513717.1	2.2e-57	97.46	autophagy-related protein 8i-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg13407 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LRP7	4.7e-42	70.18	Autophagy-related protein 8i OS=Arabidopsis thaliana OX=3702 GN=ATG8I PE=1 SV=1	[more]
Q8S925	1.7e-39	64.91	Autophagy-related protein 8h OS=Arabidopsis thaliana OX=3702 GN=ATG8H PE=1 SV=1	[more]
Q2RBS4	5.3e-38	64.10	Autophagy-related protein 8D OS=Oryza sativa subsp. japonica OX=39947 GN=ATG8D P...	[more]
Q9SL04	3.6e-34	58.41	Autophagy-related protein 8d OS=Arabidopsis thaliana OX=3702 GN=ATG8D PE=1 SV=1	[more]
Q2XPP5	4.7e-34	61.95	Autophagy-related protein 8A OS=Oryza sativa subsp. indica OX=39946 GN=ATG8A PE=...	[more]

BLAST of Carg13407 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H7R2	1.7e-58	98.31	Autophagy-related protein OS=Cucurbita moschata OX=3662 GN=LOC111461254 PE=3 SV=...	[more]
A0A6J1L0B7	8.2e-58	97.46	Autophagy-related protein OS=Cucurbita maxima OX=3661 GN=LOC111497916 PE=3 SV=1	[more]
A0A6J1GQC4	1.5e-54	92.37	Autophagy-related protein OS=Cucurbita moschata OX=3662 GN=LOC111456510 PE=3 SV=...	[more]
A0A6J1CW54	2.5e-54	90.68	Autophagy-related protein OS=Momordica charantia OX=3673 GN=LOC111014838 PE=3 SV...	[more]
A0A5D3CGI6	4.2e-54	90.68	Autophagy-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]

BLAST of Carg13407 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15580.1	3.3e-43	70.18	Ubiquitin-like superfamily protein	[more]
AT3G06420.1	1.2e-40	64.91	Ubiquitin-like superfamily protein	[more]
AT2G05630.1	2.5e-35	58.41	Ubiquitin-like superfamily protein	[more]
AT1G62040.1	8.2e-34	59.29	Ubiquitin-like superfamily protein	[more]
AT1G62040.2	8.2e-34	59.29	Ubiquitin-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004241	Autophagy protein Atg8 ubiquitin-like	PFAM	PF02991	Atg8	coord: 15..118 e-value: 5.9E-44 score: 148.5
IPR004241	Autophagy protein Atg8 ubiquitin-like	PANTHER	PTHR10969	MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED	coord: 1..118
None	No IPR available	GENE3D	3.10.20.90		coord: 1..118 e-value: 4.7E-50 score: 170.7
None	No IPR available	PANTHER	PTHR10969:SF81	AUTOPHAGY-RELATED PROTEIN	coord: 1..118
None	No IPR available	CDD	cd16108	Ubl_ATG8_like	coord: 29..113 e-value: 1.0807E-47 score: 145.799
IPR029071	Ubiquitin-like domain superfamily	SUPERFAMILY	54236	Ubiquitin-like	coord: 5..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13407-RA	Carg13407-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006914	autophagy
biological_process	GO:0006995	cellular response to nitrogen starvation
cellular_component	GO:0016020	membrane
cellular_component	GO:0005874	microtubule