Carg13244 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg13244 vs. NCBI nr
Match: KAG7027364.1 (Rop guanine nucleotide exchange factor 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 588/588 (100.00%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60
           MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS
Sbjct: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60

Query: 61  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120
           PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE
Sbjct: 61  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120

Query: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180
           DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE
Sbjct: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180

Query: 181 FTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240
           FTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG
Sbjct: 181 FTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240

Query: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA 300
           PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA
Sbjct: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA 300

Query: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360
           TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV
Sbjct: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360

Query: 361 EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420
           EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP
Sbjct: 361 EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420

Query: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS 480
           GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS
Sbjct: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS 480

Query: 481 ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAPAASPAKTTQRGFGIRKVLIDYL 540
           ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAPAASPAKTTQRGFGIRKVLIDYL
Sbjct: 481 ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAPAASPAKTTQRGFGIRKVLIDYL 540

Query: 541 GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA 589
           GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA
Sbjct: 541 GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA 588

BLAST of Carg13244 vs. NCBI nr
Match: KAG6595357.1 (Peptidyl-prolyl cis-trans isomerase PASTICCINO1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 586/588 (99.66%), Postives = 587/588 (99.83%), Query Frame = 0

Query: 1    MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60
            MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS
Sbjct: 658  MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 717

Query: 61   PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120
            PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE
Sbjct: 718  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 777

Query: 121  DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180
            DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE
Sbjct: 778  DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 837

Query: 181  FTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240
            FTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG
Sbjct: 838  FTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 897

Query: 241  PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA 300
            PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA
Sbjct: 898  PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA 957

Query: 301  TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360
            TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV
Sbjct: 958  TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 1017

Query: 361  EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420
            EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP
Sbjct: 1018 EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 1077

Query: 421  GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS 480
            GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD+QLSS
Sbjct: 1078 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDEQLSS 1137

Query: 481  ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAPAASPAKTTQRGFGIRKVLIDYL 540
            ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPA AASPAKTTQRGFGIRKVLIDYL
Sbjct: 1138 ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAQAASPAKTTQRGFGIRKVLIDYL 1197

Query: 541  GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA 589
            GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA
Sbjct: 1198 GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA 1245

BLAST of Carg13244 vs. NCBI nr
Match: XP_022931916.1 (rop guanine nucleotide exchange factor 7-like [Cucurbita moschata])

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 583/588 (99.15%), Postives = 585/588 (99.49%), Query Frame = 0

Query: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60
           MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS
Sbjct: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60

Query: 61  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120
           PPSSLSWSTRKAYGPDCIRPNV DKEEK+YVDNRKVEKNVSSSSEVEMLKERFSKLLLGE
Sbjct: 61  PPSSLSWSTRKAYGPDCIRPNVGDKEEKNYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120

Query: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180
           DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE
Sbjct: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180

Query: 181 FTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240
           FTPTWQTFPDGS HEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG
Sbjct: 181 FTPTWQTFPDGSTHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240

Query: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA 300
           PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA
Sbjct: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA 300

Query: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360
           TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV
Sbjct: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360

Query: 361 EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420
           EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP
Sbjct: 361 EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420

Query: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS 480
           GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD+QLSS
Sbjct: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDEQLSS 480

Query: 481 ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAPAASPAKTTQRGFGIRKVLIDYL 540
           ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPA AASPAKTTQRGFGIRKVLIDYL
Sbjct: 481 ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAQAASPAKTTQRGFGIRKVLIDYL 540

Query: 541 GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA 589
           GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA
Sbjct: 541 GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA 588

BLAST of Carg13244 vs. NCBI nr
Match: XP_023517044.1 (rop guanine nucleotide exchange factor 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 575/588 (97.79%), Postives = 581/588 (98.81%), Query Frame = 0

Query: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60
           MDESVVEKSQGIGHIVPENEMFSHL+EEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS
Sbjct: 1   MDESVVEKSQGIGHIVPENEMFSHLIEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60

Query: 61  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120
           PP  LSWSTRKAY PDCIRPNVADKEEK++VDNRKVEKNVSSSSEVEMLKERFSKLLLGE
Sbjct: 61  PPYLLSWSTRKAYRPDCIRPNVADKEEKNFVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120

Query: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180
           DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE
Sbjct: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180

Query: 181 FTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240
           FTPTWQTF DGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG
Sbjct: 181 FTPTWQTFLDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240

Query: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA 300
           PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSR+QLQQKRDSTNQILKAAIAINSA
Sbjct: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRKQLQQKRDSTNQILKAAIAINSA 300

Query: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360
           TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV
Sbjct: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360

Query: 361 EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420
           EASIYVWRKRTS KPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP
Sbjct: 361 EASIYVWRKRTSSKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420

Query: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS 480
           GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD+QLSS
Sbjct: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDEQLSS 480

Query: 481 ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAPAASPAKTTQRGFGIRKVLIDYL 540
           ISKLGV+SH NSPLPFSVPISGTPYKTPFATPSFSPA AASPAKTTQRGFGIRKVLIDYL
Sbjct: 481 ISKLGVMSHKNSPLPFSVPISGTPYKTPFATPSFSPAQAASPAKTTQRGFGIRKVLIDYL 540

Query: 541 GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA 589
           GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA
Sbjct: 541 GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA 588

BLAST of Carg13244 vs. NCBI nr
Match: XP_022966586.1 (rop guanine nucleotide exchange factor 7-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 573/588 (97.45%), Postives = 573/588 (97.45%), Query Frame = 0

Query: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60
           MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS
Sbjct: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60

Query: 61  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120
           PPSSLSWSTRKAYGPDCIRPNVADKEEKSY DNRKVEK VS SSEVEMLKERFSKLLLGE
Sbjct: 61  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYDDNRKVEKKVSPSSEVEMLKERFSKLLLGE 120

Query: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180
           DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE
Sbjct: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180

Query: 181 FTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240
           F PTWQTFPDGSK EVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG
Sbjct: 181 FMPTWQTFPDGSKLEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240

Query: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA 300
           PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINS 
Sbjct: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINST 300

Query: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360
           TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV
Sbjct: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360

Query: 361 EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420
           EASIYVWRKRTS KPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP
Sbjct: 361 EASIYVWRKRTSSKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420

Query: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS 480
           GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS
Sbjct: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS 480

Query: 481 ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAPAASPAKTTQRGFGIRKVLIDYL 540
           ISKLGVISH NSPL FSVPISGTPYKTPFATPSFSP  AASPAKT QRGFGIRKVLIDYL
Sbjct: 481 ISKLGVISHKNSPLHFSVPISGTPYKTPFATPSFSPVQAASPAKTAQRGFGIRKVLIDYL 540

Query: 541 GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA 589
           GIEARGGKEASNDIEK E VAYAELDKQTAQLREGV SFPSPEQGSFA
Sbjct: 541 GIEARGGKEASNDIEKLESVAYAELDKQTAQLREGVMSFPSPEQGSFA 588

BLAST of Carg13244 vs. ExPASy Swiss-Prot
Match: Q9LZN0 (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 2.3e-182
Identity = 341/495 (68.89%), Postives = 394/495 (79.60%), Query Frame = 0

Query: 26  VEEHGRESSSSSDFLTSETTGGLEEHSHSGSED-SSPPSSLSWSTRKAYGPDCIRPNVAD 85
           VEE GRESS  S   T +     EE S S +ED ++ P S  WS +          N+  
Sbjct: 11  VEEKGRESSCCSSETTRQEE---EEQSPSCTEDFTASPVSSRWSVK----------NIDG 70

Query: 86  KEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCA 145
           +++K   D+R         SEVEM+KERFSKLLLGEDMSG GNGVCTALAISNAITNLCA
Sbjct: 71  EKKKIRSDSR--------VSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCA 130

Query: 146 TLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVEFTPTWQTFPDGSKHEVMTCKQRSD 205
           TLFGQLWRLEP+  EKK  WRREM+WLLCVSDHIVE TPTWQTFPDG+K E+MTC+ RSD
Sbjct: 131 TLFGQLWRLEPLPTEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSD 190

Query: 206 LYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGI--HGPETDGSSSFHRVLQRQEDKWWL 265
           LYVNLPALRKLDNMLLEILDSFE  EFWYVDQGI  H    DGSSSF +  QRQEDKWWL
Sbjct: 191 LYVNLPALRKLDNMLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWL 250

Query: 266 PVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSATLADLEIPEPYLEALPKNGRAC 325
           PVPRV PGGLQE+SR+QLQ KRD TNQILKAA+AINS TLAD+EIPE YLE+LP+ GR+C
Sbjct: 251 PVPRVSPGGLQENSRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSC 310

Query: 326 LGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRVEASIYVWRKRTSLKPAMNSIKH 385
           LGDLIYRYISSDQFSP+CLLDCL++SSEHQA EIANRVE+SIY+W KRT+ KPA N    
Sbjct: 311 LGDLIYRYISSDQFSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATN---- 370

Query: 386 SSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFPGLPQTTLDMNKIQYNKDVGKSI 445
            +K+SWEMVKELMVD++K+EL+A+RAE+LLL LKQRFPGLPQT LDM+KIQYNKD+GKSI
Sbjct: 371 -TKTSWEMVKELMVDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSI 430

Query: 446 LESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSSISKLGVISHNNSPLPFSVPISG 505
           LESYSRVLESLAFN+VARIDDLL+VDDLT+HS  Q+   + LG  + N++P   +VP+S 
Sbjct: 431 LESYSRVLESLAFNIVARIDDLLFVDDLTRHSSDQIP--TTLG-NNGNDAPKSIAVPVSN 470

Query: 506 TPYKTPFATPSFSPA 518
                 + TPS+SP+
Sbjct: 491 ------YTTPSYSPS 470

BLAST of Carg13244 vs. ExPASy Swiss-Prot
Match: F4K295 (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 2.6e-165
Identity = 320/574 (55.75%), Postives = 407/574 (70.91%), Query Frame = 0

Query: 3   ESVVEKSQGI----GHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSED 62
           E++V+   GI     ++ P  E    L E   R S+S + + +S TT      + + S  
Sbjct: 2   ENLVKSCAGIEKKRSNLTPSME--DILTESKERSSTSGASYESSSTT------TVASSSP 61

Query: 63  SSPPSS-LSWSTRKAYGPDCIRPNV-ADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKL 122
             PPS  L W  RKA      + +V  D ++ +  D+   +    +S++VEM+KERF+KL
Sbjct: 62  PPPPSQILGWPIRKASFRKNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKL 121

Query: 123 LLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSD 182
           LLGEDMSG G GVCTALAISNAITNLCAT+FGQLWRLEP+S EKK  WRREM+W+L VSD
Sbjct: 122 LLGEDMSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSD 181

Query: 183 HIVEFTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQ 242
           HIVE TP+ QT+PDG+K EVMTC+ R DL++NLPALRKLDNMLL+IL SF+  EFWYVDQ
Sbjct: 182 HIVELTPSTQTYPDGNKFEVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQ 241

Query: 243 GIHGPETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIA 302
           GI   E DGS+SF R +QRQE+KWWLPVPR+ P GL E++R +L  KR+   QILKAA+A
Sbjct: 242 GIVASENDGSASFRRKIQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMA 301

Query: 303 INSATLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEI 362
           INS  L ++++P+ YLE LPKNGR+CLGD+IYRY++SD+FS + LLDCL++SSEH A +I
Sbjct: 302 INSLALTEMDVPKSYLETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDI 361

Query: 363 ANRVEASIYVWRKRTSLKPAMNSIKHSS--KSSWEMVKELMVDSEKMELLAERAETLLLC 422
           ANRVEASIYVWR+R   K  +N+   S+  K +WEMVKELM   +K  LL ER+ETLL C
Sbjct: 362 ANRVEASIYVWRRRVQTKLGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRC 421

Query: 423 LKQRFPGLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHS 482
           LKQRFP L QT+LD++KIQ+NKD+GKSILESYSR LESLA N++ARIDDLLYVDDLTK S
Sbjct: 422 LKQRFPSLTQTSLDISKIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQS 481

Query: 483 DQQ--LSSISKLGVISHNN-SPLPFSVPISGTPYKTPFA-TPSFSPAPAASPAK------ 542
           D    LSS +   +I+H    PLP+ +  SGTPY+T F+ TP FSP+   SP K      
Sbjct: 482 DDNNLLSSPAVSSIIAHKKVVPLPYLISASGTPYRTSFSTTPGFSPS-MISPKKGERRTP 541

Query: 543 -------------TTQRGFGIRKVLIDYLGIEAR 546
                           RG+G+R+VL +YLG+E++
Sbjct: 542 YSSKDTNKIIEKGLPSRGYGVRRVLNNYLGMESK 566

BLAST of Carg13244 vs. ExPASy Swiss-Prot
Match: Q93ZY2 (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 491.5 bits (1264), Expect = 1.3e-137
Identity = 271/512 (52.93%), Postives = 358/512 (69.92%), Query Frame = 0

Query: 34  SSSSDFLTSETTGGLEEHSHSGSEDSS--PPSSLSWSTRKAYGPDCIRPNVADKEEKSYV 93
           S S+D   SE++     H   G   SS  P S    + R  Y P  +   V   ++  + 
Sbjct: 21  SPSADISESESSSSFSCHRFDGEGASSSIPSSPRVVAGRGFYFPAPVMLPVIGGKDVVW- 80

Query: 94  DNRKVEKNVSSSSEVEMLKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLW 153
           D+++ + ++   SE+EM+KERF+KLLLGEDMSG G GVCTALAISNAITNL AT+FG+LW
Sbjct: 81  DDKQPDNDL---SEIEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELW 140

Query: 154 RLEPISEEKKVRWRREMDWLLCVSDHIVEFTPTWQTFPDGSKHEVMTCKQRSDLYVNLPA 213
           RLEP++ +KK  WRRE++WLLCVSD IVE  P+ Q FP G  +E+M  + RSDLY NLPA
Sbjct: 141 RLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGGTYEIMETRPRSDLYANLPA 200

Query: 214 LRKLDNMLLEILDSFEVCEFWYVDQGIHGPETDGSSSFHRVLQRQEDKWWLPVPRVPPGG 273
           L+KLD ML+++LD+F   EFWY D+GI   + D  S       RQEDKWWLP P+VPP G
Sbjct: 201 LKKLDAMLIDMLDAFSDTEFWYTDRGIVLGDCDKDSYNSPASVRQEDKWWLPCPKVPPNG 260

Query: 274 LQEDSRRQLQQKRDSTNQILKAAIAINSATLADLEIPEPYLEALPKNGRACLGDLIYRYI 333
           L E++R++LQQ RD  NQILKAA+AINS  LA++EIP+PYLE LPK+G+ CLG++IY+Y+
Sbjct: 261 LSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPDPYLETLPKSGKECLGEIIYQYL 320

Query: 334 SSDQFSPQCLLDCLEMSSEHQATEIANRVEASIYVWRKRTSLKPAMNSIKHSSK-----S 393
           ++++FSP+CLLDCL++SSEHQ  EIANR+EA+++VWR++       N  +H  +     S
Sbjct: 321 TANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQK-------NGRRHKKQAKLKLS 380

Query: 394 SW-EMVKELMVDSEKMELLAERAETLLLCLKQRFPGLPQTTLDMNKIQYNKDVGKSILES 453
           SW   VK L+ D+E+ + L +RAETLL  L+ RFPGLPQTTLDMNKIQYNKDVG+SILES
Sbjct: 381 SWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILES 440

Query: 454 YSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSSISKLGVISHNNSPLPFSVPISGTPY 513
           YSRV+ES+AFN+ ARIDD+LYVDD  + S     S+S L  I+  N    FSV    +P+
Sbjct: 441 YSRVMESMAFNITARIDDVLYVDDAMRRSISVTESLS-LFSINGLNPQKAFSV--QSSPH 500

Query: 514 KTPFATPSFSPA---PAASPAKTTQRGFGIRK 535
            +PFATP+ S A   P  +P   + +  G R+
Sbjct: 501 GSPFATPALSVASRSPRRAPPLYSVKRNGTRE 518

BLAST of Carg13244 vs. ExPASy Swiss-Prot
Match: Q9M056 (Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF6 PE=2 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 2.3e-129
Identity = 279/556 (50.18%), Postives = 361/556 (64.93%), Query Frame = 0

Query: 33  SSSSSDFLTSETTGGLEEHSHSGSEDSSPPSS---LSW-----STRKAYGPDCIRPNVAD 92
           SSSSS   +S     + E S      SSPP+    L W     S RK+ G          
Sbjct: 39  SSSSSGVGSSSGRSSVAERS-----VSSPPTKSQILGWPLGQGSWRKSSGK-------MK 98

Query: 93  KEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCA 152
           K+  + +D+   ++  + +SE+E+LKER +KLLLGEDMSG G GVC ALAISNAITNL A
Sbjct: 99  KKTPTKIDDFGFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYA 158

Query: 153 TLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVEFTPTWQTFPDGSKHEVMTCKQRSD 212
            + GQ WRLEPI  EKK+ WRRE++ LL VSDHIVE  P++Q FP+G+K EVM C+ RSD
Sbjct: 159 AILGQQWRLEPIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSD 218

Query: 213 LYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHGPETDGSSSFHRVLQRQEDKWWLPV 272
           L+  LPALRKLDNML+EILDSF   EFWYVDQGI   E+  S+SF    +   DKWWLP+
Sbjct: 219 LFTCLPALRKLDNMLIEILDSFGETEFWYVDQGIVAAESARSNSF----REDGDKWWLPL 278

Query: 273 PRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSATLADLEIPEPYLEALPKNGRACLG 332
           PRVP  GL E +R++L   R+ TNQILKA ++INS  LA++E+P+ YLEALPKNGR+CLG
Sbjct: 279 PRVPSDGLTEQTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKNGRSCLG 338

Query: 333 DLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRVEASIYVWRKRTSLKPAMNSIKHSS 392
           D +YR I+SD FS   LL+ +++SSE    E+ANRVEAS+YVWR+R   +  + S+  S+
Sbjct: 339 DFLYRNITSDNFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLI-SLYRST 398

Query: 393 KSSWEM-VKELMV---DSEKMELLAERAETLLLCLKQRFPGLPQTTLDMNKIQYNKDVGK 452
            + W M VKE+M+   D +K E+ AERAE+LL+ LKQRFPGL QT LD +KIQYNKDVGK
Sbjct: 399 STRWGMIVKEMMMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGK 458

Query: 453 SILESYSRVLESLAFNVVARIDDLLYVDDLTKH--SDQQLSSISKLGVISHN-------- 512
           SILESYSRVLESLA+++  RI+++L++DD++K        S   KL ++S          
Sbjct: 459 SILESYSRVLESLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGS 518

Query: 513 -----NSPLPFSVPISG--TPYKT---PFATPSFSPAPAASPAK---------------- 541
                ++P  FSV  SG  TPY+T     +TPS+SP P  SP                  
Sbjct: 519 LREKLSAPSLFSVSFSGTSTPYRTLSFSASTPSYSPMPLISPINGGRGGERAPFLSGRNI 577

BLAST of Carg13244 vs. ExPASy Swiss-Prot
Match: A4IJ27 (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 1.2e-117
Identity = 231/447 (51.68%), Postives = 312/447 (69.80%), Query Frame = 0

Query: 42  SETTGGLEEHSHSGSEDSSPPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVS 101
           SETT G  +     +   S  S  +W            P + + +    +   +++ N  
Sbjct: 47  SETTSGFSDQIDETNSFCSEASPCNW------------PVLTESKSSKCLSGLEMQSNEC 106

Query: 102 ------SSSEVEMLKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEP 161
                 S  E+E +KERF+KLLLGEDMSG G GVCTA+ ISNAITNL AT+FGQ  RLEP
Sbjct: 107 LVVQEISEPELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEP 166

Query: 162 ISEEKKVRWRREMDWLLCVSDHIVEFTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKL 221
           +  EK+  W+REM+ LL V D+IVEF P  Q   +G+  EVM  + R+D+Y+NLPALRKL
Sbjct: 167 LETEKRALWKREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKL 226

Query: 222 DNMLLEILDSFEVCEFWYVDQGIHGPETDGSS--SFHRVL-QRQEDKWWLPVPRVPPGGL 281
           D+ML+E LDSF+  EFWY ++G    ++  SS  SF +V+ QR+E+KWWLPVP VP  GL
Sbjct: 227 DSMLMEALDSFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGL 286

Query: 282 QEDSRRQLQQKRDSTNQILKAAIAINSATLADLEIPEPYLEALPKNGRACLGDLIYRYIS 341
            + +R+QL+ KR+STNQI KAA+AINS+ L+++EIP+ Y+  LPK G++ +GD IYRY+S
Sbjct: 287 SDKARKQLKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMS 346

Query: 342 -SDQFSPQCLLDCLEMSSEHQATEIANRVEASIYVWRKRTSLKPAMNSIKHSSKSSWEMV 401
            S +F P+ LLDCL +SSEH+A ++A+RVEAS+Y WR+++ L         +SK+SW MV
Sbjct: 347 GSGRFFPEQLLDCLNISSEHEAVQLADRVEASMYTWRRKSCLS--------NSKNSWNMV 406

Query: 402 KELMVDSEKME---LLAERAETLLLCLKQRFPGLPQTTLDMNKIQYNKDVGKSILESYSR 461
           K+LM  +E+ +   ++AERAETLL CLKQR+P L QT+LD+ KIQYNKDVGK++LESYSR
Sbjct: 407 KDLMSTTERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSR 466

Query: 462 VLESLAFNVVARIDDLLYVDDLTKHSD 476
           VLE LAFN+VA IDD+LYVD   + S+
Sbjct: 467 VLEGLAFNIVAWIDDVLYVDKTMRGSE 473

BLAST of Carg13244 vs. ExPASy TrEMBL
Match: A0A6J1EUY2 (rop guanine nucleotide exchange factor 7-like OS=Cucurbita moschata OX=3662 GN=LOC111438191 PE=4 SV=1)

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 583/588 (99.15%), Postives = 585/588 (99.49%), Query Frame = 0

Query: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60
           MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS
Sbjct: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60

Query: 61  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120
           PPSSLSWSTRKAYGPDCIRPNV DKEEK+YVDNRKVEKNVSSSSEVEMLKERFSKLLLGE
Sbjct: 61  PPSSLSWSTRKAYGPDCIRPNVGDKEEKNYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120

Query: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180
           DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE
Sbjct: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180

Query: 181 FTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240
           FTPTWQTFPDGS HEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG
Sbjct: 181 FTPTWQTFPDGSTHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240

Query: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA 300
           PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA
Sbjct: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA 300

Query: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360
           TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV
Sbjct: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360

Query: 361 EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420
           EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP
Sbjct: 361 EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420

Query: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS 480
           GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD+QLSS
Sbjct: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDEQLSS 480

Query: 481 ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAPAASPAKTTQRGFGIRKVLIDYL 540
           ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPA AASPAKTTQRGFGIRKVLIDYL
Sbjct: 481 ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAQAASPAKTTQRGFGIRKVLIDYL 540

Query: 541 GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA 589
           GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA
Sbjct: 541 GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA 588

BLAST of Carg13244 vs. ExPASy TrEMBL
Match: A0A6J1HPR1 (rop guanine nucleotide exchange factor 7-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466225 PE=4 SV=1)

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 573/588 (97.45%), Postives = 573/588 (97.45%), Query Frame = 0

Query: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60
           MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS
Sbjct: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60

Query: 61  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120
           PPSSLSWSTRKAYGPDCIRPNVADKEEKSY DNRKVEK VS SSEVEMLKERFSKLLLGE
Sbjct: 61  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYDDNRKVEKKVSPSSEVEMLKERFSKLLLGE 120

Query: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180
           DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE
Sbjct: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180

Query: 181 FTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240
           F PTWQTFPDGSK EVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG
Sbjct: 181 FMPTWQTFPDGSKLEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240

Query: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA 300
           PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINS 
Sbjct: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINST 300

Query: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360
           TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV
Sbjct: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360

Query: 361 EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420
           EASIYVWRKRTS KPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP
Sbjct: 361 EASIYVWRKRTSSKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420

Query: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS 480
           GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS
Sbjct: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS 480

Query: 481 ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAPAASPAKTTQRGFGIRKVLIDYL 540
           ISKLGVISH NSPL FSVPISGTPYKTPFATPSFSP  AASPAKT QRGFGIRKVLIDYL
Sbjct: 481 ISKLGVISHKNSPLHFSVPISGTPYKTPFATPSFSPVQAASPAKTAQRGFGIRKVLIDYL 540

Query: 541 GIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA 589
           GIEARGGKEASNDIEK E VAYAELDKQTAQLREGV SFPSPEQGSFA
Sbjct: 541 GIEARGGKEASNDIEKLESVAYAELDKQTAQLREGVMSFPSPEQGSFA 588

BLAST of Carg13244 vs. ExPASy TrEMBL
Match: A0A6J1HSJ5 (rop guanine nucleotide exchange factor 7-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466225 PE=4 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 554/589 (94.06%), Postives = 557/589 (94.57%), Query Frame = 0

Query: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60
           MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS
Sbjct: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60

Query: 61  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120
           PPSSLSWSTRKAYGPDCIRPNVADKEEKSY DNRKVEK VS SSEVEMLKERFSKLLLGE
Sbjct: 61  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYDDNRKVEKKVSPSSEVEMLKERFSKLLLGE 120

Query: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180
           DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE
Sbjct: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180

Query: 181 FTPTWQTFPDGSKHEVM-TCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIH 240
           F PTWQTFPDGSK EV+   KQ    Y+    +    N L EILDSFEVCEFWYVDQGIH
Sbjct: 181 FMPTWQTFPDGSKLEVLNNFKQACYGYMGFGKI----NFLQEILDSFEVCEFWYVDQGIH 240

Query: 241 GPETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINS 300
           GPETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINS
Sbjct: 241 GPETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINS 300

Query: 301 ATLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANR 360
            TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANR
Sbjct: 301 TTLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANR 360

Query: 361 VEASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRF 420
           VEASIYVWRKRTS KPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRF
Sbjct: 361 VEASIYVWRKRTSSKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRF 420

Query: 421 PGLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLS 480
           PGLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLS
Sbjct: 421 PGLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLS 480

Query: 481 SISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAPAASPAKTTQRGFGIRKVLIDY 540
           SISKLGVISH NSPL FSVPISGTPYKTPFATPSFSP  AASPAKT QRGFGIRKVLIDY
Sbjct: 481 SISKLGVISHKNSPLHFSVPISGTPYKTPFATPSFSPVQAASPAKTAQRGFGIRKVLIDY 540

Query: 541 LGIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGSFA 589
           LGIEARGGKEASNDIEK E VAYAELDKQTAQLREGV SFPSPEQGSFA
Sbjct: 541 LGIEARGGKEASNDIEKLESVAYAELDKQTAQLREGVMSFPSPEQGSFA 585

BLAST of Carg13244 vs. ExPASy TrEMBL
Match: A0A0A0KXQ7 (PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G303120 PE=4 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 4.7e-279
Identity = 493/591 (83.42%), Postives = 525/591 (88.83%), Query Frame = 0

Query: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60
           M+ES++EKSQ IGHIV ENE   HL+EEHGRESSSSSDFLTSETT GLEEHSHSGSEDSS
Sbjct: 1   MEESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETT-GLEEHSHSGSEDSS 60

Query: 61  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120
           PPSS SWSTRKA  PDCI+ N     +K++VDNRK+EK VSSSSE+EM+KERFSKLLLGE
Sbjct: 61  PPSSSSWSTRKAEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGE 120

Query: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180
           DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEP+  EKK+ WRREMDWLLCVSDHIVE
Sbjct: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVE 180

Query: 181 FTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240
            TPTWQTFPDGSK EVMTC+ RSDLYVNLPALRKLDNMLLEILDSFE CEFWYVDQGI  
Sbjct: 181 LTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILA 240

Query: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA 300
           PETDGSSS  R+LQRQE+KWWLPVPRVPPGGLQE+SRRQLQ KR+STNQILKAA+AINS 
Sbjct: 241 PETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINST 300

Query: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360
           TLADLEIPEPYLEALPKNGRACLGDLIYRYISSD FSP+CLLDCLEMSSEHQATEIANRV
Sbjct: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRV 360

Query: 361 EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420
           EASIYVWRKRT+ KPA N+ K SSKSSWEMVKELMVDSEK ELLAERAETLLLCLKQRFP
Sbjct: 361 EASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFP 420

Query: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS 480
           GLPQTTLDM+KIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD QLSS
Sbjct: 421 GLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSS 480

Query: 481 ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAPAASPA-----------KTTQRG 540
           ISKLGVISH NSPL F VPISGTPYKTPFATPSFSPA  ASPA           K  QRG
Sbjct: 481 ISKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRG 540

Query: 541 FGIRKVLIDYLGIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFP 581
           FG++KVL DYL +EARGGKE SN IEK EP+  ++L++Q +Q R GV S P
Sbjct: 541 FGVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQASQYRGGVASSP 590

BLAST of Carg13244 vs. ExPASy TrEMBL
Match: A0A5A7TPL2 (Rop guanine nucleotide exchange factor 7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G001310 PE=4 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 3.0e-278
Identity = 497/597 (83.25%), Postives = 527/597 (88.27%), Query Frame = 0

Query: 1   MDESVVEKSQGIGHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSEDSS 60
           M ES++EKSQ IGHIV ENE   HL+EEHGRESSSSSDFLTSETT GLEEHSHSGSEDSS
Sbjct: 60  MAESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETT-GLEEHSHSGSEDSS 119

Query: 61  PPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGE 120
            PSS SWSTRKA  PDCI+ N      K++VDNRK+EK VSSSSE+EM+KERFSKLLLGE
Sbjct: 120 SPSSSSWSTRKAEAPDCIKANGVVNGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGE 179

Query: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVE 180
           DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEP+  EKK+ WRREMDWLLCVSDHIVE
Sbjct: 180 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVE 239

Query: 181 FTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHG 240
            TPTWQTFPDGSK EVMTC+ RSDLYVNLPALRKLDNMLLEILDSFE CEFWYVDQGI  
Sbjct: 240 LTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILA 299

Query: 241 PETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSA 300
           PETDGSSS  R+LQRQE+KWWLPVPRVPPGGLQEDSRRQLQ KR+STNQILKAA+AINS 
Sbjct: 300 PETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQEDSRRQLQHKRESTNQILKAAMAINST 359

Query: 301 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRV 360
           TLADLEIPEPYLEALPKNGRACLGDLIYRYISSD FSP+CLLDCLEMSSEHQATEIANRV
Sbjct: 360 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRV 419

Query: 361 EASIYVWRKRTSLKPAMNSIKHSSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFP 420
           EASIYVWRKRT+ KPA N+ K SSKSSWEMVKELMVDSEK ELLAERAETLLLCLKQRFP
Sbjct: 420 EASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFP 479

Query: 421 GLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSS 480
           GLPQTTLDM+KIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD QLSS
Sbjct: 480 GLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSS 539

Query: 481 ISKLGVISHNNSPLPFSVPISGTPYKTPFATPSFSPAPAASPA-----------KTTQRG 540
           ISKLGVISH NSPL F VPISGTPYKTPFATPSFSPA  ASPA           K  QRG
Sbjct: 540 ISKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRG 599

Query: 541 FGIRKVLIDYLGIEARGGKEASNDIEKPEPVAYAELDKQTAQLREGVTSFPSPEQGS 587
           FG++KVL DYL +EARGGKE SN IEK EP+  ++L++QT+Q R GV S  SP+Q S
Sbjct: 600 FGVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQTSQYRGGVAS--SPKQRS 653

BLAST of Carg13244 vs. TAIR 10
Match: AT5G02010.1 (RHO guanyl-nucleotide exchange factor 7 )

HSP 1 Score: 640.2 bits (1650), Expect = 1.6e-183
Identity = 341/495 (68.89%), Postives = 394/495 (79.60%), Query Frame = 0

Query: 26  VEEHGRESSSSSDFLTSETTGGLEEHSHSGSED-SSPPSSLSWSTRKAYGPDCIRPNVAD 85
           VEE GRESS  S   T +     EE S S +ED ++ P S  WS +          N+  
Sbjct: 11  VEEKGRESSCCSSETTRQEE---EEQSPSCTEDFTASPVSSRWSVK----------NIDG 70

Query: 86  KEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCA 145
           +++K   D+R         SEVEM+KERFSKLLLGEDMSG GNGVCTALAISNAITNLCA
Sbjct: 71  EKKKIRSDSR--------VSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCA 130

Query: 146 TLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVEFTPTWQTFPDGSKHEVMTCKQRSD 205
           TLFGQLWRLEP+  EKK  WRREM+WLLCVSDHIVE TPTWQTFPDG+K E+MTC+ RSD
Sbjct: 131 TLFGQLWRLEPLPTEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSD 190

Query: 206 LYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGI--HGPETDGSSSFHRVLQRQEDKWWL 265
           LYVNLPALRKLDNMLLEILDSFE  EFWYVDQGI  H    DGSSSF +  QRQEDKWWL
Sbjct: 191 LYVNLPALRKLDNMLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWL 250

Query: 266 PVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSATLADLEIPEPYLEALPKNGRAC 325
           PVPRV PGGLQE+SR+QLQ KRD TNQILKAA+AINS TLAD+EIPE YLE+LP+ GR+C
Sbjct: 251 PVPRVSPGGLQENSRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSC 310

Query: 326 LGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRVEASIYVWRKRTSLKPAMNSIKH 385
           LGDLIYRYISSDQFSP+CLLDCL++SSEHQA EIANRVE+SIY+W KRT+ KPA N    
Sbjct: 311 LGDLIYRYISSDQFSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATN---- 370

Query: 386 SSKSSWEMVKELMVDSEKMELLAERAETLLLCLKQRFPGLPQTTLDMNKIQYNKDVGKSI 445
            +K+SWEMVKELMVD++K+EL+A+RAE+LLL LKQRFPGLPQT LDM+KIQYNKD+GKSI
Sbjct: 371 -TKTSWEMVKELMVDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSI 430

Query: 446 LESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSSISKLGVISHNNSPLPFSVPISG 505
           LESYSRVLESLAFN+VARIDDLL+VDDLT+HS  Q+   + LG  + N++P   +VP+S 
Sbjct: 431 LESYSRVLESLAFNIVARIDDLLFVDDLTRHSSDQIP--TTLG-NNGNDAPKSIAVPVSN 470

Query: 506 TPYKTPFATPSFSPA 518
                 + TPS+SP+
Sbjct: 491 ------YTTPSYSPS 470

BLAST of Carg13244 vs. TAIR 10
Match: AT5G05940.1 (ROP guanine nucleotide exchange factor 5 )

HSP 1 Score: 583.6 bits (1503), Expect = 1.8e-166
Identity = 320/574 (55.75%), Postives = 407/574 (70.91%), Query Frame = 0

Query: 3   ESVVEKSQGI----GHIVPENEMFSHLVEEHGRESSSSSDFLTSETTGGLEEHSHSGSED 62
           E++V+   GI     ++ P  E    L E   R S+S + + +S TT      + + S  
Sbjct: 2   ENLVKSCAGIEKKRSNLTPSME--DILTESKERSSTSGASYESSSTT------TVASSSP 61

Query: 63  SSPPSS-LSWSTRKAYGPDCIRPNV-ADKEEKSYVDNRKVEKNVSSSSEVEMLKERFSKL 122
             PPS  L W  RKA      + +V  D ++ +  D+   +    +S++VEM+KERF+KL
Sbjct: 62  PPPPSQILGWPIRKASFRKNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKL 121

Query: 123 LLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPISEEKKVRWRREMDWLLCVSD 182
           LLGEDMSG G GVCTALAISNAITNLCAT+FGQLWRLEP+S EKK  WRREM+W+L VSD
Sbjct: 122 LLGEDMSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSD 181

Query: 183 HIVEFTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKLDNMLLEILDSFEVCEFWYVDQ 242
           HIVE TP+ QT+PDG+K EVMTC+ R DL++NLPALRKLDNMLL+IL SF+  EFWYVDQ
Sbjct: 182 HIVELTPSTQTYPDGNKFEVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQ 241

Query: 243 GIHGPETDGSSSFHRVLQRQEDKWWLPVPRVPPGGLQEDSRRQLQQKRDSTNQILKAAIA 302
           GI   E DGS+SF R +QRQE+KWWLPVPR+ P GL E++R +L  KR+   QILKAA+A
Sbjct: 242 GIVASENDGSASFRRKIQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMA 301

Query: 303 INSATLADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPQCLLDCLEMSSEHQATEI 362
           INS  L ++++P+ YLE LPKNGR+CLGD+IYRY++SD+FS + LLDCL++SSEH A +I
Sbjct: 302 INSLALTEMDVPKSYLETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDI 361

Query: 363 ANRVEASIYVWRKRTSLKPAMNSIKHSS--KSSWEMVKELMVDSEKMELLAERAETLLLC 422
           ANRVEASIYVWR+R   K  +N+   S+  K +WEMVKELM   +K  LL ER+ETLL C
Sbjct: 362 ANRVEASIYVWRRRVQTKLGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRC 421

Query: 423 LKQRFPGLPQTTLDMNKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHS 482
           LKQRFP L QT+LD++KIQ+NKD+GKSILESYSR LESLA N++ARIDDLLYVDDLTK S
Sbjct: 422 LKQRFPSLTQTSLDISKIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQS 481

Query: 483 DQQ--LSSISKLGVISHNN-SPLPFSVPISGTPYKTPFA-TPSFSPAPAASPAK------ 542
           D    LSS +   +I+H    PLP+ +  SGTPY+T F+ TP FSP+   SP K      
Sbjct: 482 DDNNLLSSPAVSSIIAHKKVVPLPYLISASGTPYRTSFSTTPGFSPS-MISPKKGERRTP 541

Query: 543 -------------TTQRGFGIRKVLIDYLGIEAR 546
                           RG+G+R+VL +YLG+E++
Sbjct: 542 YSSKDTNKIIEKGLPSRGYGVRRVLNNYLGMESK 566

BLAST of Carg13244 vs. TAIR 10
Match: AT4G38430.1 (rho guanyl-nucleotide exchange factor 1 )

HSP 1 Score: 491.5 bits (1264), Expect = 9.4e-139
Identity = 271/512 (52.93%), Postives = 358/512 (69.92%), Query Frame = 0

Query: 34  SSSSDFLTSETTGGLEEHSHSGSEDSS--PPSSLSWSTRKAYGPDCIRPNVADKEEKSYV 93
           S S+D   SE++     H   G   SS  P S    + R  Y P  +   V   ++  + 
Sbjct: 21  SPSADISESESSSSFSCHRFDGEGASSSIPSSPRVVAGRGFYFPAPVMLPVIGGKDVVW- 80

Query: 94  DNRKVEKNVSSSSEVEMLKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLW 153
           D+++ + ++   SE+EM+KERF+KLLLGEDMSG G GVCTALAISNAITNL AT+FG+LW
Sbjct: 81  DDKQPDNDL---SEIEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELW 140

Query: 154 RLEPISEEKKVRWRREMDWLLCVSDHIVEFTPTWQTFPDGSKHEVMTCKQRSDLYVNLPA 213
           RLEP++ +KK  WRRE++WLLCVSD IVE  P+ Q FP G  +E+M  + RSDLY NLPA
Sbjct: 141 RLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGGTYEIMETRPRSDLYANLPA 200

Query: 214 LRKLDNMLLEILDSFEVCEFWYVDQGIHGPETDGSSSFHRVLQRQEDKWWLPVPRVPPGG 273
           L+KLD ML+++LD+F   EFWY D+GI   + D  S       RQEDKWWLP P+VPP G
Sbjct: 201 LKKLDAMLIDMLDAFSDTEFWYTDRGIVLGDCDKDSYNSPASVRQEDKWWLPCPKVPPNG 260

Query: 274 LQEDSRRQLQQKRDSTNQILKAAIAINSATLADLEIPEPYLEALPKNGRACLGDLIYRYI 333
           L E++R++LQQ RD  NQILKAA+AINS  LA++EIP+PYLE LPK+G+ CLG++IY+Y+
Sbjct: 261 LSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPDPYLETLPKSGKECLGEIIYQYL 320

Query: 334 SSDQFSPQCLLDCLEMSSEHQATEIANRVEASIYVWRKRTSLKPAMNSIKHSSK-----S 393
           ++++FSP+CLLDCL++SSEHQ  EIANR+EA+++VWR++       N  +H  +     S
Sbjct: 321 TANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQK-------NGRRHKKQAKLKLS 380

Query: 394 SW-EMVKELMVDSEKMELLAERAETLLLCLKQRFPGLPQTTLDMNKIQYNKDVGKSILES 453
           SW   VK L+ D+E+ + L +RAETLL  L+ RFPGLPQTTLDMNKIQYNKDVG+SILES
Sbjct: 381 SWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILES 440

Query: 454 YSRVLESLAFNVVARIDDLLYVDDLTKHSDQQLSSISKLGVISHNNSPLPFSVPISGTPY 513
           YSRV+ES+AFN+ ARIDD+LYVDD  + S     S+S L  I+  N    FSV    +P+
Sbjct: 441 YSRVMESMAFNITARIDDVLYVDDAMRRSISVTESLS-LFSINGLNPQKAFSV--QSSPH 500

Query: 514 KTPFATPSFSPA---PAASPAKTTQRGFGIRK 535
            +PFATP+ S A   P  +P   + +  G R+
Sbjct: 501 GSPFATPALSVASRSPRRAPPLYSVKRNGTRE 518

BLAST of Carg13244 vs. TAIR 10
Match: AT3G55660.1 (ROP (rho of plants) guanine nucleotide exchange factor 6 )

HSP 1 Score: 464.2 bits (1193), Expect = 1.6e-130
Identity = 279/556 (50.18%), Postives = 361/556 (64.93%), Query Frame = 0

Query: 33  SSSSSDFLTSETTGGLEEHSHSGSEDSSPPSS---LSW-----STRKAYGPDCIRPNVAD 92
           SSSSS   +S     + E S      SSPP+    L W     S RK+ G          
Sbjct: 39  SSSSSGVGSSSGRSSVAERS-----VSSPPTKSQILGWPLGQGSWRKSSGK-------MK 98

Query: 93  KEEKSYVDNRKVEKNVSSSSEVEMLKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCA 152
           K+  + +D+   ++  + +SE+E+LKER +KLLLGEDMSG G GVC ALAISNAITNL A
Sbjct: 99  KKTPTKIDDFGFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYA 158

Query: 153 TLFGQLWRLEPISEEKKVRWRREMDWLLCVSDHIVEFTPTWQTFPDGSKHEVMTCKQRSD 212
            + GQ WRLEPI  EKK+ WRRE++ LL VSDHIVE  P++Q FP+G+K EVM C+ RSD
Sbjct: 159 AILGQQWRLEPIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSD 218

Query: 213 LYVNLPALRKLDNMLLEILDSFEVCEFWYVDQGIHGPETDGSSSFHRVLQRQEDKWWLPV 272
           L+  LPALRKLDNML+EILDSF   EFWYVDQGI   E+  S+SF    +   DKWWLP+
Sbjct: 219 LFTCLPALRKLDNMLIEILDSFGETEFWYVDQGIVAAESARSNSF----REDGDKWWLPL 278

Query: 273 PRVPPGGLQEDSRRQLQQKRDSTNQILKAAIAINSATLADLEIPEPYLEALPKNGRACLG 332
           PRVP  GL E +R++L   R+ TNQILKA ++INS  LA++E+P+ YLEALPKNGR+CLG
Sbjct: 279 PRVPSDGLTEQTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKNGRSCLG 338

Query: 333 DLIYRYISSDQFSPQCLLDCLEMSSEHQATEIANRVEASIYVWRKRTSLKPAMNSIKHSS 392
           D +YR I+SD FS   LL+ +++SSE    E+ANRVEAS+YVWR+R   +  + S+  S+
Sbjct: 339 DFLYRNITSDNFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLI-SLYRST 398

Query: 393 KSSWEM-VKELMV---DSEKMELLAERAETLLLCLKQRFPGLPQTTLDMNKIQYNKDVGK 452
            + W M VKE+M+   D +K E+ AERAE+LL+ LKQRFPGL QT LD +KIQYNKDVGK
Sbjct: 399 STRWGMIVKEMMMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGK 458

Query: 453 SILESYSRVLESLAFNVVARIDDLLYVDDLTKH--SDQQLSSISKLGVISHN-------- 512
           SILESYSRVLESLA+++  RI+++L++DD++K        S   KL ++S          
Sbjct: 459 SILESYSRVLESLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGS 518

Query: 513 -----NSPLPFSVPISG--TPYKT---PFATPSFSPAPAASPAK---------------- 541
                ++P  FSV  SG  TPY+T     +TPS+SP P  SP                  
Sbjct: 519 LREKLSAPSLFSVSFSGTSTPYRTLSFSASTPSYSPMPLISPINGGRGGERAPFLSGRNI 577

BLAST of Carg13244 vs. TAIR 10
Match: AT4G00460.2 (RHO guanyl-nucleotide exchange factor 3 )

HSP 1 Score: 425.2 bits (1092), Expect = 8.3e-119
Identity = 231/447 (51.68%), Postives = 312/447 (69.80%), Query Frame = 0

Query: 42  SETTGGLEEHSHSGSEDSSPPSSLSWSTRKAYGPDCIRPNVADKEEKSYVDNRKVEKNVS 101
           SETT G  +     +   S  S  +W            P + + +    +   +++ N  
Sbjct: 47  SETTSGFSDQIDETNSFCSEASPCNW------------PVLTESKSSKCLSGLEMQSNEC 106

Query: 102 ------SSSEVEMLKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEP 161
                 S  E+E +KERF+KLLLGEDMSG G GVCTA+ ISNAITNL AT+FGQ  RLEP
Sbjct: 107 LVVQEISEPELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEP 166

Query: 162 ISEEKKVRWRREMDWLLCVSDHIVEFTPTWQTFPDGSKHEVMTCKQRSDLYVNLPALRKL 221
           +  EK+  W+REM+ LL V D+IVEF P  Q   +G+  EVM  + R+D+Y+NLPALRKL
Sbjct: 167 LETEKRALWKREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKL 226

Query: 222 DNMLLEILDSFEVCEFWYVDQGIHGPETDGSS--SFHRVL-QRQEDKWWLPVPRVPPGGL 281
           D+ML+E LDSF+  EFWY ++G    ++  SS  SF +V+ QR+E+KWWLPVP VP  GL
Sbjct: 227 DSMLMEALDSFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGL 286

Query: 282 QEDSRRQLQQKRDSTNQILKAAIAINSATLADLEIPEPYLEALPKNGRACLGDLIYRYIS 341
            + +R+QL+ KR+STNQI KAA+AINS+ L+++EIP+ Y+  LPK G++ +GD IYRY+S
Sbjct: 287 SDKARKQLKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMS 346

Query: 342 -SDQFSPQCLLDCLEMSSEHQATEIANRVEASIYVWRKRTSLKPAMNSIKHSSKSSWEMV 401
            S +F P+ LLDCL +SSEH+A ++A+RVEAS+Y WR+++ L         +SK+SW MV
Sbjct: 347 GSGRFFPEQLLDCLNISSEHEAVQLADRVEASMYTWRRKSCLS--------NSKNSWNMV 406

Query: 402 KELMVDSEKME---LLAERAETLLLCLKQRFPGLPQTTLDMNKIQYNKDVGKSILESYSR 461
           K+LM  +E+ +   ++AERAETLL CLKQR+P L QT+LD+ KIQYNKDVGK++LESYSR
Sbjct: 407 KDLMSTTERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSR 466

Query: 462 VLESLAFNVVARIDDLLYVDDLTKHSD 476
           VLE LAFN+VA IDD+LYVD   + S+
Sbjct: 467 VLEGLAFNIVAWIDDVLYVDKTMRGSE 473