Carg13005 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg13005 vs. NCBI nr
Match: KAG7023652.1 (K(+) efflux antiporter 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1133.2 bits (2930), Expect = 0.0e+00
Identity = 619/619 (100.00%), Postives = 619/619 (100.00%), Query Frame = 0

Query: 1   SFFPPSTHSESEATATATAPGRPPFFFCAVSAMRLPFSASLVLLFFVHLLLYFATFPSLS 60
           SFFPPSTHSESEATATATAPGRPPFFFCAVSAMRLPFSASLVLLFFVHLLLYFATFPSLS
Sbjct: 1   SFFPPSTHSESEATATATAPGRPPFFFCAVSAMRLPFSASLVLLFFVHLLLYFATFPSLS 60

Query: 61  ISLLTVTTSELVAGEINATADSNFSRSANVDDSFANIIDRALEREFTENEQSDEVTDAGS 120
           ISLLTVTTSELVAGEINATADSNFSRSANVDDSFANIIDRALEREFTENEQSDEVTDAGS
Sbjct: 61  ISLLTVTTSELVAGEINATADSNFSRSANVDDSFANIIDRALEREFTENEQSDEVTDAGS 120

Query: 121 FNNSVAEKQAVLETVARVKSKKNESKEEKSFQFHDVFHLDNENRGEDMPTLIDRKDNVFI 180
           FNNSVAEKQAVLETVARVKSKKNESKEEKSFQFHDVFHLDNENRGEDMPTLIDRKDNVFI
Sbjct: 121 FNNSVAEKQAVLETVARVKSKKNESKEEKSFQFHDVFHLDNENRGEDMPTLIDRKDNVFI 180

Query: 181 ISNPKSKFPMLQLDLRLISDLVVVIVSATCGGIAFACAGQPVFTGYLLAGSLIGPGGLSC 240
           ISNPKSKFPMLQLDLRLISDLVVVIVSATCGGIAFACAGQPVFTGYLLAGSLIGPGGLSC
Sbjct: 181 ISNPKSKFPMLQLDLRLISDLVVVIVSATCGGIAFACAGQPVFTGYLLAGSLIGPGGLSC 240

Query: 241 VSEMVQVETVAQFGVIFLLFALGLEFSTAKLRVVRAVAIVGGLLQIFLFMCLCGITASLC 300
           VSEMVQVETVAQFGVIFLLFALGLEFSTAKLRVVRAVAIVGGLLQIFLFMCLCGITASLC
Sbjct: 241 VSEMVQVETVAQFGVIFLLFALGLEFSTAKLRVVRAVAIVGGLLQIFLFMCLCGITASLC 300

Query: 301 GGKSSEGVFVGAFLSMSSTAVVLKFLMERNSVNAIHGQVTIGTLILQDCAVGLLFALLPI 360
           GGKSSEGVFVGAFLSMSSTAVVLKFLMERNSVNAIHGQVTIGTLILQDCAVGLLFALLPI
Sbjct: 301 GGKSSEGVFVGAFLSMSSTAVVLKFLMERNSVNAIHGQVTIGTLILQDCAVGLLFALLPI 360

Query: 361 LGGTSGVLQGVLSMTKSLVVLITFLITLTILSRTCVPRFLKLMISLSSQTNELYQLAAVA 420
           LGGTSGVLQGVLSMTKSLVVLITFLITLTILSRTCVPRFLKLMISLSSQTNELYQLAAVA
Sbjct: 361 LGGTSGVLQGVLSMTKSLVVLITFLITLTILSRTCVPRFLKLMISLSSQTNELYQLAAVA 420

Query: 421 FCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTLEQVEPIRNFFAALFLASIGMLIH 480
           FCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTLEQVEPIRNFFAALFLASIGMLIH
Sbjct: 421 FCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTLEQVEPIRNFFAALFLASIGMLIH 480

Query: 481 VHFLWNHIDILLAAVILVIVIKTVVVTAVVKGFGYSNKTSLLVGMSLAQIGEFAFVLLSR 540
           VHFLWNHIDILLAAVILVIVIKTVVVTAVVKGFGYSNKTSLLVGMSLAQIGEFAFVLLSR
Sbjct: 481 VHFLWNHIDILLAAVILVIVIKTVVVTAVVKGFGYSNKTSLLVGMSLAQIGEFAFVLLSR 540

Query: 541 ASNLHLVEGKLYILLLGTTALSLVTTPLLFKLIPAVVRIGVLLRWFSPDGLSEIGFKGDG 600
           ASNLHLVEGKLYILLLGTTALSLVTTPLLFKLIPAVVRIGVLLRWFSPDGLSEIGFKGDG
Sbjct: 541 ASNLHLVEGKLYILLLGTTALSLVTTPLLFKLIPAVVRIGVLLRWFSPDGLSEIGFKGDG 600

Query: 601 LRTDGAKRITLVVQGSHFS 620
           LRTDGAKRITLVVQGSHFS
Sbjct: 601 LRTDGAKRITLVVQGSHFS 619

BLAST of Carg13005 vs. NCBI nr
Match: XP_022961032.1 (K(+) efflux antiporter 4-like [Cucurbita moschata] >XP_023516132.1 K(+) efflux antiporter 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1067.8 bits (2760), Expect = 3.6e-308
Identity = 584/587 (99.49%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 33  MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD 92
           MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD
Sbjct: 1   MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD 60

Query: 93  SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ 152
           SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ
Sbjct: 61  SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ 120

Query: 153 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG 212
           FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFP+LQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPVLQLDLRLISDLVVVIVSATCGG 180

Query: 213 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 272
           IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 240

Query: 273 VVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 332
           VVRAVA+VGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV
Sbjct: 241 VVRAVAVVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 300

Query: 333 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 392
           NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS
Sbjct: 301 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 360

Query: 393 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 452
           RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 453 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 512
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 480

Query: 513 FGYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 572
           FGY+NKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYTNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 540

Query: 573 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS 620
           IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS
Sbjct: 541 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS 587

BLAST of Carg13005 vs. NCBI nr
Match: XP_022987663.1 (K(+) efflux antiporter 4-like [Cucurbita maxima])

HSP 1 Score: 1060.8 bits (2742), Expect = 4.4e-306
Identity = 581/587 (98.98%), Postives = 585/587 (99.66%), Query Frame = 0

Query: 33  MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD 92
           MRLPFSASLVLLFFVHLLLYFATFPSLSISL+TVTTSELVAGEINATADSNFSRSANVDD
Sbjct: 1   MRLPFSASLVLLFFVHLLLYFATFPSLSISLVTVTTSELVAGEINATADSNFSRSANVDD 60

Query: 93  SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ 152
           SFANIIDRALEREFTENEQ+DEV DAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ
Sbjct: 61  SFANIIDRALEREFTENEQTDEVADAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ 120

Query: 153 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG 212
           FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG 180

Query: 213 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 272
           IAFACAGQPVFTGYLLAGSLIGPGGLS VSEMVQVETVAQFGVIFLLFALGLEFSTAKLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 240

Query: 273 VVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 332
           VVRAVA+VGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV
Sbjct: 241 VVRAVAVVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 300

Query: 333 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 392
           NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS
Sbjct: 301 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 360

Query: 393 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 452
           RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 453 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 512
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 480

Query: 513 FGYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 572
           FGY+NKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYNNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 540

Query: 573 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS 620
           IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS
Sbjct: 541 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS 587

BLAST of Carg13005 vs. NCBI nr
Match: KAG6589989.1 (K(+) efflux antiporter 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1056.6 bits (2731), Expect = 8.3e-305
Identity = 579/582 (99.48%), Postives = 581/582 (99.83%), Query Frame = 0

Query: 33  MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD 92
           MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD
Sbjct: 1   MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD 60

Query: 93  SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ 152
           SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ
Sbjct: 61  SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ 120

Query: 153 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG 212
           FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG 180

Query: 213 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 272
           IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 240

Query: 273 VVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 332
           VVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV
Sbjct: 241 VVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 300

Query: 333 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 392
           NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS
Sbjct: 301 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 360

Query: 393 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 452
           RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 453 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 512
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 480

Query: 513 FGYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 572
           FGY+NKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYTNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 540

Query: 573 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQ 615
           IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITL +Q
Sbjct: 541 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLCIQ 582

BLAST of Carg13005 vs. NCBI nr
Match: XP_038879505.1 (K(+) efflux antiporter 4 [Benincasa hispida])

HSP 1 Score: 1007.3 bits (2603), Expect = 5.8e-290
Identity = 556/587 (94.72%), Postives = 566/587 (96.42%), Query Frame = 0

Query: 33  MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD 92
           MRLP SA LVL  F HLL  FATFPSLSISLLTVT SELV GEINATADSN SRSAN D 
Sbjct: 1   MRLPSSAILVLFLFFHLLC-FATFPSLSISLLTVTKSELVTGEINATADSNSSRSANDDH 60

Query: 93  SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ 152
           SFANIIDRALEREFTENEQ+DEV DAGSFN+SVAEKQAVLETVARVKSKKNESKEEKSFQ
Sbjct: 61  SFANIIDRALEREFTENEQTDEVADAGSFNSSVAEKQAVLETVARVKSKKNESKEEKSFQ 120

Query: 153 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG 212
           FHDVFHLDNENR EDMPTLIDRKDNVFIISNPKSK+P+LQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFHLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180

Query: 213 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 272
           IAFACAGQPVFTGYLLAGSLIGPGGLS VSEMVQVETVAQFGVIFLLFALGLEFST KLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240

Query: 273 VVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 332
           VVRAVA++GGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV
Sbjct: 241 VVRAVAVLGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 300

Query: 333 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 392
           NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSL+VLITFLI LTILS
Sbjct: 301 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLLVLITFLIILTILS 360

Query: 393 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 452
           RTCVPRFLKLM+SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPRFLKLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 453 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 512
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVI+IKTVVVT VVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIIIKTVVVTTVVKG 480

Query: 513 FGYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 572
           FGY+NKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYNNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 540

Query: 573 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS 620
           IPAVVRIGVLLRWFSPDGLSEIGFKGDG RTDGAKRITLVVQ SH S
Sbjct: 541 IPAVVRIGVLLRWFSPDGLSEIGFKGDGFRTDGAKRITLVVQDSHVS 586

BLAST of Carg13005 vs. ExPASy Swiss-Prot
Match: Q9ZUN3 (K(+) efflux antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=KEA4 PE=2 SV=2)

HSP 1 Score: 808.9 bits (2088), Expect = 3.9e-233
Identity = 461/584 (78.94%), Postives = 511/584 (87.50%), Query Frame = 0

Query: 37  FSASLVLLFFVHLLLYFATFPSLSISLLTVT-TSELVAGEINAT-ADSNFSRSANVDDSF 96
           FS   + L  + L+  F  F S + S  +   T  +V  EIN T  +SN + +   +DSF
Sbjct: 11  FSVITMRLLTLLLICTFFFFFSFAYSAESDNETDSVVTREINGTVVESNATSAKPREDSF 70

Query: 97  ANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNE--SKEEKSFQ 156
           A++IDRALE+EF +N+Q +EV D GSFNNSVA++QAVLETVARVK KKNE  +KEEKSF 
Sbjct: 71  ADMIDRALEKEFPDNDQ-NEVPDPGSFNNSVADQQAVLETVARVKPKKNETKTKEEKSF- 130

Query: 157 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG 216
               F+LDNEN  ED P LIDRKDNVFI+SNPKSK+P+LQLDLRLISDLVVVIVSATCGG
Sbjct: 131 ----FNLDNENGVEDTPRLIDRKDNVFIMSNPKSKYPVLQLDLRLISDLVVVIVSATCGG 190

Query: 217 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 276
           IAFACAGQPV TGYLLAGS+IGPGGLS VSEMVQVETVAQFGVIFLLFALGLEFS AKLR
Sbjct: 191 IAFACAGQPVITGYLLAGSIIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSAAKLR 250

Query: 277 VVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 336
           VVRAVAI GGLLQIFLFMCL GITASLCGGK +EG+FVGAFLSMSSTAVVLKFLMERNS+
Sbjct: 251 VVRAVAIPGGLLQIFLFMCLSGITASLCGGKLTEGIFVGAFLSMSSTAVVLKFLMERNSI 310

Query: 337 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 396
           +A+HGQ+T+GTLILQDCAVGLLFALLP+LGGTSGVLQGVLSM KSL +LI FL  L +LS
Sbjct: 311 SALHGQITVGTLILQDCAVGLLFALLPVLGGTSGVLQGVLSMAKSLAILIAFLGALFVLS 370

Query: 397 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 456
           RT VP FLKLM SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 371 RTWVPWFLKLMTSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 430

Query: 457 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 516
           AQHTLEQVEPIRNFFAALFLASIGMLIH+HFLWNH+DILLAAV+LVIVIKTVVV  VVK 
Sbjct: 431 AQHTLEQVEPIRNFFAALFLASIGMLIHMHFLWNHVDILLAAVLLVIVIKTVVVAIVVKV 490

Query: 517 FGYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 576
           FGY+NKT++LVGMSLAQIGEFAFVLLSRASNLHL+E KLY+LLLGTTALSLVTTPLLFKL
Sbjct: 491 FGYNNKTAVLVGMSLAQIGEFAFVLLSRASNLHLIESKLYLLLLGTTALSLVTTPLLFKL 550

Query: 577 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGS 617
           IPAVV +GVLLRWFSPD  +EIGFKG+   ++ AKRI+L++QGS
Sbjct: 551 IPAVVHLGVLLRWFSPDSSTEIGFKGELYHSESAKRISLMIQGS 588

BLAST of Carg13005 vs. ExPASy Swiss-Prot
Match: B5X0N6 (K(+) efflux antiporter 6 OS=Arabidopsis thaliana OX=3702 GN=KEA6 PE=2 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 9.7e-224
Identity = 437/586 (74.57%), Postives = 508/586 (86.69%), Query Frame = 0

Query: 39  ASLVLLFFVHLLLYFATFPSLSISLL---TVTTSELVAGEINATADSNFSRSANVDDSFA 98
           A L+LL    L    A+  ++S S L   TV  S      +NA++ S+  +    + SFA
Sbjct: 18  ALLLLLLSFFLCFSVASPRAISDSDLLDETVANSSSSVASLNASSSSSLVKPK--EGSFA 77

Query: 99  NIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKS-KKNESKEEKSFQFH 158
           +IIDRALE+EF E++Q +EV D GSFNNSVA +QAVLETVARVKS KKNE+KEEK FQ H
Sbjct: 78  DIIDRALEKEFNESDQ-NEVADPGSFNNSVAGQQAVLETVARVKSTKKNETKEEKRFQLH 137

Query: 159 DVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGGIA 218
           DVF+L+N+NR ED PTLIDRKDNVFIISN KSK+P+LQLDLRLISDLVVVIVSATCGGIA
Sbjct: 138 DVFNLNNDNRAEDTPTLIDRKDNVFIISNSKSKYPVLQLDLRLISDLVVVIVSATCGGIA 197

Query: 219 FACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLRVV 278
           FACAGQPV TGYLLAGS+IGPGGL+ +SEMVQVETVAQFGV+FLLFALGLEFSTAKL+VV
Sbjct: 198 FACAGQPVITGYLLAGSIIGPGGLNFISEMVQVETVAQFGVVFLLFALGLEFSTAKLKVV 257

Query: 279 RAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSVNA 338
           R+VA++GGLLQI LFM LCGIT SLCGGK SEGVFVGAFLSMSSTAVVLKFLME+NS N+
Sbjct: 258 RSVAVLGGLLQILLFMFLCGITVSLCGGKRSEGVFVGAFLSMSSTAVVLKFLMEKNSTNS 317

Query: 339 IHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILSRT 398
           +HGQVTIG LILQDCAVGLLFALLP+L G SG++ G+LS+ K +V+L++FL  L+ILSRT
Sbjct: 318 LHGQVTIGILILQDCAVGLLFALLPVLEGNSGIVHGMLSIGKVVVLLLSFLAVLSILSRT 377

Query: 399 CVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQ 458
           C+P  LKLM+SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLA+
Sbjct: 378 CIPWLLKLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAE 437

Query: 459 HTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKGFG 518
           HTLEQ+EPIRN FAALFLASIGML++VHFLW H+DILLA+VILVI+IKT +VT VVKGFG
Sbjct: 438 HTLEQIEPIRNLFAALFLASIGMLVNVHFLWTHVDILLASVILVIIIKTTIVTTVVKGFG 497

Query: 519 YSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKLIP 578
           Y+NKT+LLVG+SLAQIGEFAFVLLSRASNLHL+EGKLY+LLLGTTALSLVTTPL+FK+IP
Sbjct: 498 YNNKTALLVGISLAQIGEFAFVLLSRASNLHLIEGKLYLLLLGTTALSLVTTPLVFKMIP 557

Query: 579 AVVRIGVLLRWFSPDGLSEIGFKGDGLRTD-GAKRITLVVQGSHFS 620
           AVV +G+LL+WFSPD   E   KG+ +R++ G +R+ L+ + SH S
Sbjct: 558 AVVHLGILLKWFSPDSTIE---KGEIVRSESGKQRMILMSRQSHSS 597

BLAST of Carg13005 vs. ExPASy Swiss-Prot
Match: Q8VYR9 (K(+) efflux antiporter 5 OS=Arabidopsis thaliana OX=3702 GN=KEA5 PE=2 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 4.9e-183
Identity = 363/551 (65.88%), Postives = 441/551 (80.04%), Query Frame = 0

Query: 54  ATFPSLSISLLTVTTSELVAGEINATADS------NFSRSANVDDSFANIIDRALEREFT 113
           A F  + ++ L +  + L A     T +       N +   N + S A + DR LE+EF+
Sbjct: 2   ARFAVIGLTFLLLLGTSLSARSDEETRERFYGNVVNSTAPGNGEGSIAKMFDRVLEKEFS 61

Query: 114 ENEQSDEVTDAGSFNNSVAEKQAVLETVARV---KSKKNESKEE---KSFQFHDVFHLDN 173
           EN+ S E +D  SFN+SVA++QA +ETVA+V   K K+N+++E    + FQ  DVF L+N
Sbjct: 62  END-SPEGSDGASFNSSVADQQAEIETVAKVTHEKGKRNDTQENNGTRPFQLQDVFSLEN 121

Query: 174 ENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGGIAFACAGQP 233
           E+  +DM TLID+K+NVF++SN KSK+P+LQ+DLRLISDLVV+IV A  GGI F+C GQP
Sbjct: 122 ED-SDDM-TLIDKKNNVFVMSNKKSKYPILQVDLRLISDLVVIIVFAAIGGIVFSCLGQP 181

Query: 234 VFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLRVVRAVAIVG 293
           V  GYLLAGS+IGPGGL  +SEMVQVETVAQFGV+FLLFALGLEFS  KL+VV  VA++G
Sbjct: 182 VIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSMTKLKVVGPVAVLG 241

Query: 294 GLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSVNAIHGQVTI 353
           GLLQI L M LCG+TA LCG + SEG+FVGAFLSMSSTAVV+KFL+ERNS +++HGQVTI
Sbjct: 242 GLLQIVLLMFLCGVTALLCGARLSEGIFVGAFLSMSSTAVVVKFLVERNSTSSLHGQVTI 301

Query: 354 GTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILSRTCVPRFLK 413
           G LI QDC VGLLFALLP+LGG SG+LQG++SM K L++L  +L   ++L+ + VPRFLK
Sbjct: 302 GILIFQDCVVGLLFALLPVLGGNSGLLQGIISMGKLLLILSIYLTVASLLTWSFVPRFLK 361

Query: 414 LMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTLEQVE 473
           LMI LSSQTNELYQLAAVAFCLL AWCSDKLGLSLELGSF AGVM+STT+ AQHTLEQVE
Sbjct: 362 LMIQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMLSTTEFAQHTLEQVE 421

Query: 474 PIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKGFGYSNKTSL 533
           PIRN FAALFL+SIGMLI+VHFLWNH+DILLA+VILVIVIKT +   VVK F Y+ + S 
Sbjct: 422 PIRNLFAALFLSSIGMLINVHFLWNHVDILLASVILVIVIKTAIAAVVVKAFRYNMRISF 481

Query: 534 LVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKLIPAVVRIGV 593
            VG+ LAQIGEFAFVLLSRASNLH++EGK+Y+LLLGTTALSLVTTPLLFKLIP+ + +GV
Sbjct: 482 HVGVLLAQIGEFAFVLLSRASNLHVIEGKMYLLLLGTTALSLVTTPLLFKLIPSAMNLGV 541

BLAST of Carg13005 vs. ExPASy Swiss-Prot
Match: Q8BH01 (Transmembrane and coiled-coil domain-containing protein 3 OS=Mus musculus OX=10090 GN=Tmco3 PE=2 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.2e-41
Identity = 146/496 (29.44%), Postives = 260/496 (52.42%), Query Frame = 0

Query: 96  NIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQFHD 155
           +++D  LE      ++ +E  +  +F+++ + K    E V RV+ ++  SK+  + +   
Sbjct: 178 SMLDEILEDVRKAADRLEEEIEEHAFDDNKSVKGVNFEAVLRVEEEEASSKQNMTKR--- 237

Query: 156 VFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVV-IVSATCGGIA 215
                    G  +  LID ++N +I++ P+    + + D   I D+V + ++S  CG + 
Sbjct: 238 -----EVEDGLGLSMLIDSQNNQYILTKPRDS-TIPRADHHFIKDIVTIGMLSLPCGWLC 297

Query: 216 FACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLRVV 275
            A  G P   GY++ G L+GP GL+ +  +VQVET+ +FGV F LF +GLEFS  KLR V
Sbjct: 298 TA-IGLPTMFGYIICGVLLGPSGLNSIKSIVQVETLGEFGVFFTLFLVGLEFSPEKLRKV 357

Query: 276 RAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLM-----ER 335
             +++ G      L +        L   + ++ VF+   LS+SST +V +FL+     ++
Sbjct: 358 WRISLQGPCYMTLLMIAFGLWWGHLLRIRPTQSVFISTCLSLSSTPLVSRFLVGSARGDK 417

Query: 336 NSVNAIHGQVTIGTLILQDCAVGLLFALLPIL-----GGTSGVLQGVLSMTKSLVVLI-- 395
            + +  +  V +G L++QD  +GL  A++P L     G +S V+  VL +   +  ++  
Sbjct: 418 EAGDIDYSTVLLGMLVMQDVQLGLFIAVMPTLIQAGAGASSSVVMEVLRILFLIGQILFS 477

Query: 396 ---TFLITLTILSRTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELG 455
               FL+ L + +    P + KL +  S    E+  L   AF  L+   ++ L +S+ELG
Sbjct: 478 LAAVFLLCLVMKTYLIGPYYRKLHLE-SKGNKEILVLGVSAFTFLMLTVTELLDVSMELG 537

Query: 456 SFAAGVMISTTD--LAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVIL 515
            F AG ++S+    + +  +  +EPIR+F A +F ASIG+ +   F+   + +L+   + 
Sbjct: 538 CFLAGALVSSQGHMVTEEIMTYIEPIRDFLAIIFFASIGLHVFPTFVIYELTVLVFLTLS 597

Query: 516 VIVIKTVVVTAVVKGF--GYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILL 572
           V+++K V+   V+       S     +V   LAQ+ EF+FVL SRA    ++  ++Y+L+
Sbjct: 598 VVIMKFVLAVLVLSLILPRSSQYIKWIVSAGLAQVSEFSFVLGSRARRAGILSREVYLLI 657

BLAST of Carg13005 vs. ExPASy Swiss-Prot
Match: Q6UWJ1 (Transmembrane and coiled-coil domain-containing protein 3 OS=Homo sapiens OX=9606 GN=TMCO3 PE=1 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 7.9e-40
Identity = 148/497 (29.78%), Postives = 255/497 (51.31%), Query Frame = 0

Query: 96  NIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQFHD 155
           +++D  LE      ++ +E  +  +F+++ + K    E V RV+ ++  SK+        
Sbjct: 178 SMLDEILEDVRKAADRLEEEIEEHAFDDNKSVKGVNFEAVLRVEEEEANSKQ-------- 237

Query: 156 VFHLDNENRGED--MPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVV-IVSATCGG 215
             ++      +D  +  LID ++N +I++ P+    + + D   I D+V + ++S  CG 
Sbjct: 238 --NITKREVEDDLGLSMLIDSQNNQYILTKPRDS-TIPRADHHFIKDIVTIGMLSLPCGW 297

Query: 216 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 275
           +  A  G P   GY++ G L+GP GL+ +  +VQVET+ +FGV F LF +GLEFS  KLR
Sbjct: 298 LCTA-IGLPTMFGYIICGVLLGPSGLNSIKSIVQVETLGEFGVFFTLFLVGLEFSPEKLR 357

Query: 276 VVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMER--- 335
            V  +++ G      L +    +   L   K ++ VF+   LS+SST +V +FLM     
Sbjct: 358 KVWKISLQGPCYMTLLMIAFGLLWGHLLRIKPTQSVFISTCLSLSSTPLVSRFLMGSARG 417

Query: 336 -NSVNAIHGQVTIGTLILQDCAVGLLFALLPIL--GGTSGVLQGVLSMTKSLVVL----- 395
               +  +  V +G L+ QD  +GL  A++P L   G S     V+ + + LV++     
Sbjct: 418 DKEGDIDYSTVLLGMLVTQDVQLGLFMAVMPTLIQAGASASSSIVVEVLRILVLIGQILF 477

Query: 396 ---ITFLITLTILSRTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLEL 455
                FL+ L I      P + KL +  S    E+  L   AF  L+   ++ L +S+EL
Sbjct: 478 SLAAVFLLCLVIKKYLIGPYYRKLHME-SKGNKEILILGISAFIFLMLTVTELLDVSMEL 537

Query: 456 GSFAAGVMISTTD--LAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVI 515
           G F AG ++S+    + +     +EPIR+F A +F ASIG+ +   F+   + +L+   +
Sbjct: 538 GCFLAGALVSSQGPVVTEEIATSIEPIRDFLAIVFFASIGLHVFPTFVAYELTVLVFLTL 597

Query: 516 LVIVIKTVVVTAVVKGF--GYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYIL 572
            V+V+K ++   V+       S     +V   LAQ+ EF+FVL SRA    ++  ++Y+L
Sbjct: 598 SVVVMKFLLAALVLSLILPRSSQYIKWIVSAGLAQVSEFSFVLGSRARRAGVISREVYLL 657

BLAST of Carg13005 vs. ExPASy TrEMBL
Match: A0A6J1H913 (K(+) efflux antiporter 4-like OS=Cucurbita moschata OX=3662 GN=LOC111461659 PE=4 SV=1)

HSP 1 Score: 1067.8 bits (2760), Expect = 1.7e-308
Identity = 584/587 (99.49%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 33  MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD 92
           MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD
Sbjct: 1   MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD 60

Query: 93  SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ 152
           SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ
Sbjct: 61  SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ 120

Query: 153 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG 212
           FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFP+LQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPVLQLDLRLISDLVVVIVSATCGG 180

Query: 213 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 272
           IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 240

Query: 273 VVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 332
           VVRAVA+VGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV
Sbjct: 241 VVRAVAVVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 300

Query: 333 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 392
           NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS
Sbjct: 301 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 360

Query: 393 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 452
           RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 453 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 512
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 480

Query: 513 FGYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 572
           FGY+NKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYTNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 540

Query: 573 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS 620
           IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS
Sbjct: 541 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS 587

BLAST of Carg13005 vs. ExPASy TrEMBL
Match: A0A6J1JK39 (K(+) efflux antiporter 4-like OS=Cucurbita maxima OX=3661 GN=LOC111485149 PE=4 SV=1)

HSP 1 Score: 1060.8 bits (2742), Expect = 2.1e-306
Identity = 581/587 (98.98%), Postives = 585/587 (99.66%), Query Frame = 0

Query: 33  MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD 92
           MRLPFSASLVLLFFVHLLLYFATFPSLSISL+TVTTSELVAGEINATADSNFSRSANVDD
Sbjct: 1   MRLPFSASLVLLFFVHLLLYFATFPSLSISLVTVTTSELVAGEINATADSNFSRSANVDD 60

Query: 93  SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ 152
           SFANIIDRALEREFTENEQ+DEV DAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ
Sbjct: 61  SFANIIDRALEREFTENEQTDEVADAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ 120

Query: 153 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG 212
           FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG 180

Query: 213 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 272
           IAFACAGQPVFTGYLLAGSLIGPGGLS VSEMVQVETVAQFGVIFLLFALGLEFSTAKLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 240

Query: 273 VVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 332
           VVRAVA+VGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV
Sbjct: 241 VVRAVAVVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 300

Query: 333 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 392
           NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS
Sbjct: 301 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 360

Query: 393 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 452
           RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 453 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 512
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 480

Query: 513 FGYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 572
           FGY+NKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYNNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 540

Query: 573 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS 620
           IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS
Sbjct: 541 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS 587

BLAST of Carg13005 vs. ExPASy TrEMBL
Match: A0A1S3BQ67 (K(+) efflux antiporter 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492311 PE=4 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.2e-288
Identity = 551/587 (93.87%), Postives = 561/587 (95.57%), Query Frame = 0

Query: 33  MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD 92
           MRL  S  LVL  F HLLL FATFPSLSISL+TVT SELV GEINATADSN SRSAN D 
Sbjct: 1   MRLSLSTILVLFLFFHLLLCFATFPSLSISLITVTKSELVPGEINATADSNSSRSANDDH 60

Query: 93  SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ 152
           SFANIIDRALEREFTENEQ+DEV D GSFNNSVAEKQAVLETVARVKSKKNE+KEEKSFQ
Sbjct: 61  SFANIIDRALEREFTENEQTDEVADPGSFNNSVAEKQAVLETVARVKSKKNETKEEKSFQ 120

Query: 153 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG 212
           FHDVFHLDNENR EDMPTLIDRKDNVFIISNPKSK+P+LQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFHLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180

Query: 213 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 272
           IAFACAGQPVFTGYLLAGSLIGPGGLS VSEMVQVETVAQFGVIFLLFALGLEFST KLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240

Query: 273 VVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 332
           VVRAVA++GGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV
Sbjct: 241 VVRAVAVLGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 300

Query: 333 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 392
           NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLI FLI LTI S
Sbjct: 301 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLIAFLIILTIFS 360

Query: 393 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 452
           RTCVPRFLKLM+SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPRFLKLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 453 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 512
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVI+IKTVVVT VVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIIIKTVVVTTVVKG 480

Query: 513 FGYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 572
           FGY+NKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYNNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 540

Query: 573 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS 620
           IPAVVRIGVLLRWFSPDGLSEIGFKGD  RTDGAKRITLVVQ SH S
Sbjct: 541 IPAVVRIGVLLRWFSPDGLSEIGFKGDAFRTDGAKRITLVVQDSHVS 587

BLAST of Carg13005 vs. ExPASy TrEMBL
Match: A0A0A0M1W9 (Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G586880 PE=4 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 1.1e-286
Identity = 547/587 (93.19%), Postives = 559/587 (95.23%), Query Frame = 0

Query: 33  MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD 92
           MRL  S  LVL  F HLLL FATFP+LSISL+TVT SELV GEINATADSN SRS N D 
Sbjct: 1   MRLSLSRILVLFLFFHLLLCFATFPTLSISLITVTKSELVPGEINATADSNSSRSDNDDH 60

Query: 93  SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEKSFQ 152
           SFANIIDRALEREFTENEQ+DEV D GSFNNSVAEKQAVLETVARVKSKKNE+KEEKSFQ
Sbjct: 61  SFANIIDRALEREFTENEQTDEVADPGSFNNSVAEKQAVLETVARVKSKKNETKEEKSFQ 120

Query: 153 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG 212
           FHDVFHLDNENR EDMPTLIDRKDNVFIISNPKSK+P+LQLDLRLISDLVVVIVSATCGG
Sbjct: 121 FHDVFHLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSATCGG 180

Query: 213 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 272
           IAFACAGQPVFTGYLLAGSLIGPGGLS VSEMVQVETVAQFGVIFLLFALGLEFST KLR
Sbjct: 181 IAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240

Query: 273 VVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 332
           VVRAVA++GGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV
Sbjct: 241 VVRAVAVLGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 300

Query: 333 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 392
           NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLI FLI LTI S
Sbjct: 301 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLIAFLIILTIFS 360

Query: 393 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 452
           RTCVPRFLKLM+SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPRFLKLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 453 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 512
           AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVT VVKG
Sbjct: 421 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTTVVKG 480

Query: 513 FGYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 572
           FGY+NKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL
Sbjct: 481 FGYNNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 540

Query: 573 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS 620
           IPAVVRIGVLLRWFSPDG SEIGFKGD  RTDGAKRITLV+Q +H S
Sbjct: 541 IPAVVRIGVLLRWFSPDGFSEIGFKGDAFRTDGAKRITLVIQDAHVS 587

BLAST of Carg13005 vs. ExPASy TrEMBL
Match: A0A5D3CEZ1 (K(+) efflux antiporter 4 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002470 PE=4 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 9.3e-286
Identity = 549/590 (93.05%), Postives = 559/590 (94.75%), Query Frame = 0

Query: 33  MRLPFSASLVLLFFVHLLLYFATFPSLSISLLTVTTSELVAGEINATADSNFSRSANVDD 92
           MRL  S  LVL  F HLLL FATFPSLSISL+TVT SELV GEINATADSN SRSAN D 
Sbjct: 1   MRLSLSTILVLFLFFHLLLCFATFPSLSISLITVTKSELVPGEINATADSNSSRSANDDH 60

Query: 93  SFANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNESKEEK--- 152
           SFANIIDRALEREFTENEQ+DEV D GSFNNSVAEKQAVLETVARVKSKKNE+KEEK   
Sbjct: 61  SFANIIDRALEREFTENEQTDEVADPGSFNNSVAEKQAVLETVARVKSKKNETKEEKYVL 120

Query: 153 SFQFHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSAT 212
            F FHDVFHLDNENR EDMPTLIDRKDNVFIISNPKSK+P+LQLDLRLISDLVVVIVSAT
Sbjct: 121 PFPFHDVFHLDNENRAEDMPTLIDRKDNVFIISNPKSKYPVLQLDLRLISDLVVVIVSAT 180

Query: 213 CGGIAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTA 272
           CGGIAFACAGQPVFTGYLLAGSLIGPGGLS VSEMVQVETVAQFGVIFLLFALGLEFST 
Sbjct: 181 CGGIAFACAGQPVFTGYLLAGSLIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSTT 240

Query: 273 KLRVVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMER 332
           KLRVVRAVA++GGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMER
Sbjct: 241 KLRVVRAVAVLGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMER 300

Query: 333 NSVNAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLT 392
           NSVNAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLI FLI LT
Sbjct: 301 NSVNAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLIAFLIILT 360

Query: 393 ILSRTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMIST 452
           I SRTCVPRFLKLM+SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMIST
Sbjct: 361 IFSRTCVPRFLKLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMIST 420

Query: 453 TDLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAV 512
           TDLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVI+IKTVVVT V
Sbjct: 421 TDLAQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIIIKTVVVTTV 480

Query: 513 VKGFGYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLL 572
           VKGFGY+NKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLL
Sbjct: 481 VKGFGYNNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLL 540

Query: 573 FKLIPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGSHFS 620
           FKLIPAVVRIGVLLRWFSPDGLSEIGFKGD  RTDGAKRITLVVQ SH S
Sbjct: 541 FKLIPAVVRIGVLLRWFSPDGLSEIGFKGDAFRTDGAKRITLVVQDSHVS 590

BLAST of Carg13005 vs. TAIR 10
Match: AT2G19600.1 (K+ efflux antiporter 4 )

HSP 1 Score: 808.9 bits (2088), Expect = 2.8e-234
Identity = 461/584 (78.94%), Postives = 511/584 (87.50%), Query Frame = 0

Query: 37  FSASLVLLFFVHLLLYFATFPSLSISLLTVT-TSELVAGEINAT-ADSNFSRSANVDDSF 96
           FS   + L  + L+  F  F S + S  +   T  +V  EIN T  +SN + +   +DSF
Sbjct: 11  FSVITMRLLTLLLICTFFFFFSFAYSAESDNETDSVVTREINGTVVESNATSAKPREDSF 70

Query: 97  ANIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKSKKNE--SKEEKSFQ 156
           A++IDRALE+EF +N+Q +EV D GSFNNSVA++QAVLETVARVK KKNE  +KEEKSF 
Sbjct: 71  ADMIDRALEKEFPDNDQ-NEVPDPGSFNNSVADQQAVLETVARVKPKKNETKTKEEKSF- 130

Query: 157 FHDVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGG 216
               F+LDNEN  ED P LIDRKDNVFI+SNPKSK+P+LQLDLRLISDLVVVIVSATCGG
Sbjct: 131 ----FNLDNENGVEDTPRLIDRKDNVFIMSNPKSKYPVLQLDLRLISDLVVVIVSATCGG 190

Query: 217 IAFACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLR 276
           IAFACAGQPV TGYLLAGS+IGPGGLS VSEMVQVETVAQFGVIFLLFALGLEFS AKLR
Sbjct: 191 IAFACAGQPVITGYLLAGSIIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSAAKLR 250

Query: 277 VVRAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSV 336
           VVRAVAI GGLLQIFLFMCL GITASLCGGK +EG+FVGAFLSMSSTAVVLKFLMERNS+
Sbjct: 251 VVRAVAIPGGLLQIFLFMCLSGITASLCGGKLTEGIFVGAFLSMSSTAVVLKFLMERNSI 310

Query: 337 NAIHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILS 396
           +A+HGQ+T+GTLILQDCAVGLLFALLP+LGGTSGVLQGVLSM KSL +LI FL  L +LS
Sbjct: 311 SALHGQITVGTLILQDCAVGLLFALLPVLGGTSGVLQGVLSMAKSLAILIAFLGALFVLS 370

Query: 397 RTCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 456
           RT VP FLKLM SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 371 RTWVPWFLKLMTSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 430

Query: 457 AQHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKG 516
           AQHTLEQVEPIRNFFAALFLASIGMLIH+HFLWNH+DILLAAV+LVIVIKTVVV  VVK 
Sbjct: 431 AQHTLEQVEPIRNFFAALFLASIGMLIHMHFLWNHVDILLAAVLLVIVIKTVVVAIVVKV 490

Query: 517 FGYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKL 576
           FGY+NKT++LVGMSLAQIGEFAFVLLSRASNLHL+E KLY+LLLGTTALSLVTTPLLFKL
Sbjct: 491 FGYNNKTAVLVGMSLAQIGEFAFVLLSRASNLHLIESKLYLLLLGTTALSLVTTPLLFKL 550

Query: 577 IPAVVRIGVLLRWFSPDGLSEIGFKGDGLRTDGAKRITLVVQGS 617
           IPAVV +GVLLRWFSPD  +EIGFKG+   ++ AKRI+L++QGS
Sbjct: 551 IPAVVHLGVLLRWFSPDSSTEIGFKGELYHSESAKRISLMIQGS 588

BLAST of Carg13005 vs. TAIR 10
Match: AT5G11800.1 (K+ efflux antiporter 6 )

HSP 1 Score: 777.7 bits (2007), Expect = 6.9e-225
Identity = 437/586 (74.57%), Postives = 508/586 (86.69%), Query Frame = 0

Query: 39  ASLVLLFFVHLLLYFATFPSLSISLL---TVTTSELVAGEINATADSNFSRSANVDDSFA 98
           A L+LL    L    A+  ++S S L   TV  S      +NA++ S+  +    + SFA
Sbjct: 18  ALLLLLLSFFLCFSVASPRAISDSDLLDETVANSSSSVASLNASSSSSLVKPK--EGSFA 77

Query: 99  NIIDRALEREFTENEQSDEVTDAGSFNNSVAEKQAVLETVARVKS-KKNESKEEKSFQFH 158
           +IIDRALE+EF E++Q +EV D GSFNNSVA +QAVLETVARVKS KKNE+KEEK FQ H
Sbjct: 78  DIIDRALEKEFNESDQ-NEVADPGSFNNSVAGQQAVLETVARVKSTKKNETKEEKRFQLH 137

Query: 159 DVFHLDNENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGGIA 218
           DVF+L+N+NR ED PTLIDRKDNVFIISN KSK+P+LQLDLRLISDLVVVIVSATCGGIA
Sbjct: 138 DVFNLNNDNRAEDTPTLIDRKDNVFIISNSKSKYPVLQLDLRLISDLVVVIVSATCGGIA 197

Query: 219 FACAGQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLRVV 278
           FACAGQPV TGYLLAGS+IGPGGL+ +SEMVQVETVAQFGV+FLLFALGLEFSTAKL+VV
Sbjct: 198 FACAGQPVITGYLLAGSIIGPGGLNFISEMVQVETVAQFGVVFLLFALGLEFSTAKLKVV 257

Query: 279 RAVAIVGGLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSVNA 338
           R+VA++GGLLQI LFM LCGIT SLCGGK SEGVFVGAFLSMSSTAVVLKFLME+NS N+
Sbjct: 258 RSVAVLGGLLQILLFMFLCGITVSLCGGKRSEGVFVGAFLSMSSTAVVLKFLMEKNSTNS 317

Query: 339 IHGQVTIGTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILSRT 398
           +HGQVTIG LILQDCAVGLLFALLP+L G SG++ G+LS+ K +V+L++FL  L+ILSRT
Sbjct: 318 LHGQVTIGILILQDCAVGLLFALLPVLEGNSGIVHGMLSIGKVVVLLLSFLAVLSILSRT 377

Query: 399 CVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQ 458
           C+P  LKLM+SLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLA+
Sbjct: 378 CIPWLLKLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAE 437

Query: 459 HTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKGFG 518
           HTLEQ+EPIRN FAALFLASIGML++VHFLW H+DILLA+VILVI+IKT +VT VVKGFG
Sbjct: 438 HTLEQIEPIRNLFAALFLASIGMLVNVHFLWTHVDILLASVILVIIIKTTIVTTVVKGFG 497

Query: 519 YSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKLIP 578
           Y+NKT+LLVG+SLAQIGEFAFVLLSRASNLHL+EGKLY+LLLGTTALSLVTTPL+FK+IP
Sbjct: 498 YNNKTALLVGISLAQIGEFAFVLLSRASNLHLIEGKLYLLLLGTTALSLVTTPLVFKMIP 557

Query: 579 AVVRIGVLLRWFSPDGLSEIGFKGDGLRTD-GAKRITLVVQGSHFS 620
           AVV +G+LL+WFSPD   E   KG+ +R++ G +R+ L+ + SH S
Sbjct: 558 AVVHLGILLKWFSPDSTIE---KGEIVRSESGKQRMILMSRQSHSS 597

BLAST of Carg13005 vs. TAIR 10
Match: AT5G51710.1 (K+ efflux antiporter 5 )

HSP 1 Score: 642.5 bits (1656), Expect = 3.5e-184
Identity = 363/551 (65.88%), Postives = 441/551 (80.04%), Query Frame = 0

Query: 54  ATFPSLSISLLTVTTSELVAGEINATADS------NFSRSANVDDSFANIIDRALEREFT 113
           A F  + ++ L +  + L A     T +       N +   N + S A + DR LE+EF+
Sbjct: 2   ARFAVIGLTFLLLLGTSLSARSDEETRERFYGNVVNSTAPGNGEGSIAKMFDRVLEKEFS 61

Query: 114 ENEQSDEVTDAGSFNNSVAEKQAVLETVARV---KSKKNESKEE---KSFQFHDVFHLDN 173
           EN+ S E +D  SFN+SVA++QA +ETVA+V   K K+N+++E    + FQ  DVF L+N
Sbjct: 62  END-SPEGSDGASFNSSVADQQAEIETVAKVTHEKGKRNDTQENNGTRPFQLQDVFSLEN 121

Query: 174 ENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGGIAFACAGQP 233
           E+  +DM TLID+K+NVF++SN KSK+P+LQ+DLRLISDLVV+IV A  GGI F+C GQP
Sbjct: 122 ED-SDDM-TLIDKKNNVFVMSNKKSKYPILQVDLRLISDLVVIIVFAAIGGIVFSCLGQP 181

Query: 234 VFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLRVVRAVAIVG 293
           V  GYLLAGS+IGPGGL  +SEMVQVETVAQFGV+FLLFALGLEFS  KL+VV  VA++G
Sbjct: 182 VIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSMTKLKVVGPVAVLG 241

Query: 294 GLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSVNAIHGQVTI 353
           GLLQI L M LCG+TA LCG + SEG+FVGAFLSMSSTAVV+KFL+ERNS +++HGQVTI
Sbjct: 242 GLLQIVLLMFLCGVTALLCGARLSEGIFVGAFLSMSSTAVVVKFLVERNSTSSLHGQVTI 301

Query: 354 GTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILSRTCVPRFLK 413
           G LI QDC VGLLFALLP+LGG SG+LQG++SM K L++L  +L   ++L+ + VPRFLK
Sbjct: 302 GILIFQDCVVGLLFALLPVLGGNSGLLQGIISMGKLLLILSIYLTVASLLTWSFVPRFLK 361

Query: 414 LMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTLEQVE 473
           LMI LSSQTNELYQLAAVAFCLL AWCSDKLGLSLELGSF AGVM+STT+ AQHTLEQVE
Sbjct: 362 LMIQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMLSTTEFAQHTLEQVE 421

Query: 474 PIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKGFGYSNKTSL 533
           PIRN FAALFL+SIGMLI+VHFLWNH+DILLA+VILVIVIKT +   VVK F Y+ + S 
Sbjct: 422 PIRNLFAALFLSSIGMLINVHFLWNHVDILLASVILVIVIKTAIAAVVVKAFRYNMRISF 481

Query: 534 LVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKLIPAVVRIGV 593
            VG+ LAQIGEFAFVLLSRASNLH++EGK+Y+LLLGTTALSLVTTPLLFKLIP+ + +GV
Sbjct: 482 HVGVLLAQIGEFAFVLLSRASNLHVIEGKMYLLLLGTTALSLVTTPLLFKLIPSAMNLGV 541

BLAST of Carg13005 vs. TAIR 10
Match: AT5G51710.2 (K+ efflux antiporter 5 )

HSP 1 Score: 642.5 bits (1656), Expect = 3.5e-184
Identity = 363/551 (65.88%), Postives = 441/551 (80.04%), Query Frame = 0

Query: 54  ATFPSLSISLLTVTTSELVAGEINATADS------NFSRSANVDDSFANIIDRALEREFT 113
           A F  + ++ L +  + L A     T +       N +   N + S A + DR LE+EF+
Sbjct: 2   ARFAVIGLTFLLLLGTSLSARSDEETRERFYGNVVNSTAPGNGEGSIAKMFDRVLEKEFS 61

Query: 114 ENEQSDEVTDAGSFNNSVAEKQAVLETVARV---KSKKNESKEE---KSFQFHDVFHLDN 173
           EN+ S E +D  SFN+SVA++QA +ETVA+V   K K+N+++E    + FQ  DVF L+N
Sbjct: 62  END-SPEGSDGASFNSSVADQQAEIETVAKVTHEKGKRNDTQENNGTRPFQLQDVFSLEN 121

Query: 174 ENRGEDMPTLIDRKDNVFIISNPKSKFPMLQLDLRLISDLVVVIVSATCGGIAFACAGQP 233
           E+  +DM TLID+K+NVF++SN KSK+P+LQ+DLRLISDLVV+IV A  GGI F+C GQP
Sbjct: 122 ED-SDDM-TLIDKKNNVFVMSNKKSKYPILQVDLRLISDLVVIIVFAAIGGIVFSCLGQP 181

Query: 234 VFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLRVVRAVAIVG 293
           V  GYLLAGS+IGPGGL  +SEMVQVETVAQFGV+FLLFALGLEFS  KL+VV  VA++G
Sbjct: 182 VIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSMTKLKVVGPVAVLG 241

Query: 294 GLLQIFLFMCLCGITASLCGGKSSEGVFVGAFLSMSSTAVVLKFLMERNSVNAIHGQVTI 353
           GLLQI L M LCG+TA LCG + SEG+FVGAFLSMSSTAVV+KFL+ERNS +++HGQVTI
Sbjct: 242 GLLQIVLLMFLCGVTALLCGARLSEGIFVGAFLSMSSTAVVVKFLVERNSTSSLHGQVTI 301

Query: 354 GTLILQDCAVGLLFALLPILGGTSGVLQGVLSMTKSLVVLITFLITLTILSRTCVPRFLK 413
           G LI QDC VGLLFALLP+LGG SG+LQG++SM K L++L  +L   ++L+ + VPRFLK
Sbjct: 302 GILIFQDCVVGLLFALLPVLGGNSGLLQGIISMGKLLLILSIYLTVASLLTWSFVPRFLK 361

Query: 414 LMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTLEQVE 473
           LMI LSSQTNELYQLAAVAFCLL AWCSDKLGLSLELGSF AGVM+STT+ AQHTLEQVE
Sbjct: 362 LMIQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMLSTTEFAQHTLEQVE 421

Query: 474 PIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKGFGYSNKTSL 533
           PIRN FAALFL+SIGMLI+VHFLWNH+DILLA+VILVIVIKT +   VVK F Y+ + S 
Sbjct: 422 PIRNLFAALFLSSIGMLINVHFLWNHVDILLASVILVIVIKTAIAAVVVKAFRYNMRISF 481

Query: 534 LVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLLFKLIPAVVRIGV 593
            VG+ LAQIGEFAFVLLSRASNLH++EGK+Y+LLLGTTALSLVTTPLLFKLIP+ + +GV
Sbjct: 482 HVGVLLAQIGEFAFVLLSRASNLHVIEGKMYLLLLGTTALSLVTTPLLFKLIPSAMNLGV 541

BLAST of Carg13005 vs. TAIR 10
Match: AT1G01790.1 (K+ efflux antiporter 1 )

HSP 1 Score: 137.1 bits (344), Expect = 4.7e-32
Identity = 112/356 (31.46%), Postives = 183/356 (51.40%), Query Frame = 0

Query: 219 GQPVFTGYLLAGSLIGPGGLSCVSEMVQVETVAQFGVIFLLFALGLEFSTAKLRVVRAVA 278
           G PV  GYL AG LIGP GLS +  +     +A+FGV+FLLF +GLE S  +L  ++   
Sbjct: 612 GSPVL-GYLAAGILIGPYGLSIIRNVHGTRAIAEFGVVFLLFNIGLELSVERLSSMKKYV 671

Query: 279 IVGGLLQIFLFMCLCGITASLCGGKSS-EGVFVGAFLSMSSTAVVLKFLMERNSVNAIHG 338
              G  Q+ +   + G+ A    G++    + +G  L++SSTAVVL+ L ER    + HG
Sbjct: 672 FGLGSAQVLVTAAVVGLLAHYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHG 731

Query: 339 QVTIGTLILQDCAVGLLFALLPILGGTS---GV-LQGVLSMTKSLVVLITFLITLTILSR 398
           + +   L+ QD AV +L  L+P++   S   G+  Q +        V     IT  I   
Sbjct: 732 RASFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKAAVAITAIIAGG 791

Query: 399 TCVPRFLKLMISLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLA 458
             + R +   I+  ++  E++    +   L  +  + + GLS+ LG+F AG++++ T+ +
Sbjct: 792 RLLLRPIYKQIA-ENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFS 851

Query: 459 QHTLEQVEPIRNFFAALFLASIGMLIHVHFLWNHIDILLAAVILVIVIKTVVVTAVVKGF 518
                 + P R     LF  ++GM I    L ++  +++  + L+IV KT++V  + K F
Sbjct: 852 LQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVIVGTLGLLIVGKTMLVVIMGKLF 911

Query: 519 GYSNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYILLLGTTALSLVTTPLL 570
           G S  +++ VG+ LA  GEFAFV    A N  ++  +L  LL     +S+  TP L
Sbjct: 912 GISIISAIRVGLLLAPGGEFAFVAFGEAVNQGIMSPQLSSLLFLVVGISMAITPWL 965