Carg12976 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12976
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Threonine synthase
Location	Carg_Chr10: 3435324 .. 3436928 (-)
RNA-Seq Expression	Carg12976
Synteny	Carg12976

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCCTCTTCGCTGTTAAACCCCGCCATTTCTTTCCCCTCTTACAAGCCCAAGCTCCAAGCCAACCCAAAGTTCCGAAATCCCATTCTCAGAATCGGACGAGTATCCGCCACTTCCGATTCGTCTTCCTCCACGCCGACTAATGGCTCTCCGTCATTGTCGACCACCAAGAACCGCCGCTCTGCCGACGAGAACATCAAAGACGAAGCACGGCGCCATCGTGCAGCCTCCGGAGAAGACCGTAGCAACTTCTCGGCCAAGTACGTGCCATTTAACGCCGGCGCCGACTCGTCTGAGTGGTACTCGCTTGACGAGGTCGTTTACCGGAGCCGATCTGGTGGATTGCTCGACGTTCAGCACGACATGGAGGCCTTGAAGAAATTCGACGGCGCGTACTGGCGCAACCTCTTCGATTCACGCGTGGGGAAAACCACATGGCCTTATGGGTCCGGTGTGTGGAGCAAGAAGGAGTGGGTGTTGCCGGAGATTGACCCGGACGACATCGTCAGCGCCTTCGAAGGTAACTCGAACCTGTTTTGGGCTGAGAGATTTGGCAAACAGTTTCTGGGTATGAATGATTTATGGGTAAAACACTGTGGGATTAGCCACACTGGGAGTTTCAAGGATTTGGGCATGACTGTTTTGGTTAGCCAAGTGAACCGGCTGAGGAAATTGAAACGCCCCGTCGTCGGAGTCGGTTGTGCATCGACCGGAGACACATCGGCCGCGCTCTCAGCCTACTGCGCATCTGCAGGGATCCCTTCAATCGTCTTCCTCCCTGCAAACAAGATTTCCATGGCACAGTTAGTTCAGCCCATTGCAAATGGAGCCTTTGTGTTGAGTATAGACACTGATTTTGATGGCTGCATGAAGCTAATTAGGGAAGTTACAGCAGAATTGCCCATTTACTTAGCCAATTCCTTGAATAGTTTGAGGCTTGAAGGGCAAAAGACGGCTGCCATTGAAATTCTACAGCAGTTTGATTGGGCAGTACCTGAATGGGTGATCATTCCAGGAGGCAATCTTGGGAACATATATGCATTTTACAAAGGATTCAAGATGTGCCAAGAGCTTGGCCTTGTTGACAGGATTCCTAGGCTTGTTTGTGCACAAGCAGCCAATGCTAACCCTCTGTATTTACACTACAAATCTGGGTGGAAAGATTTCAAGGCTGTGAAAGCAAGTTCGACGTTCGCCTCGGCCATTCAGATTGGGGATCCTGTTTCCATAGATAGAGCTGTTTGTGCTCTACAGAACTCGAATGGCATCGTCGAGGAAGCGACAGAGGAGGAACTAATGGATGCAATGGCACAAGCTGACTCTACTGGGATGTTCATTTGCCCTCACACAGGGGTGGCACTAACGGCTTTGATCAAGCTGAGAAACCAAGGTGTCATCAGCCCAACCGATAGGACGGTGGTGGTGAGCACTGCTCATGGCCTGAAGTTTACACAGTCGAAAATCGACTATCACTCCAAGGAAATCCCAGACATGGCTTGCCAATTTGCTAACCCACCTGTTCAAGTGAAGGCTGATTATGGGAGTGTCATGGATGTACTTAAAGATTATTTACTACAAAAGTCTCCAATGTCTAAAGTGTAG

mRNA sequence

ATGGCTGCCTCTTCGCTGTTAAACCCCGCCATTTCTTTCCCCTCTTACAAGCCCAAGCTCCAAGCCAACCCAAAGTTCCGAAATCCCATTCTCAGAATCGGACGAGTATCCGCCACTTCCGATTCGTCTTCCTCCACGCCGACTAATGGCTCTCCGTCATTGTCGACCACCAAGAACCGCCGCTCTGCCGACGAGAACATCAAAGACGAAGCACGGCGCCATCGTGCAGCCTCCGGAGAAGACCGTAGCAACTTCTCGGCCAAGTACGTGCCATTTAACGCCGGCGCCGACTCGTCTGAGTGGTACTCGCTTGACGAGGTCGTTTACCGGAGCCGATCTGGTGGATTGCTCGACGTTCAGCACGACATGGAGGCCTTGAAGAAATTCGACGGCGCGTACTGGCGCAACCTCTTCGATTCACGCGTGGGGAAAACCACATGGCCTTATGGGTCCGGTGTGTGGAGCAAGAAGGAGTGGGTGTTGCCGGAGATTGACCCGGACGACATCGTCAGCGCCTTCGAAGGTAACTCGAACCTGTTTTGGGCTGAGAGATTTGGCAAACAGTTTCTGGGTATGAATGATTTATGGGTAAAACACTGTGGGATTAGCCACACTGGGAGTTTCAAGGATTTGGGCATGACTGTTTTGGTTAGCCAAGTGAACCGGCTGAGGAAATTGAAACGCCCCGTCGTCGGAGTCGGTTGTGCATCGACCGGAGACACATCGGCCGCGCTCTCAGCCTACTGCGCATCTGCAGGGATCCCTTCAATCGTCTTCCTCCCTGCAAACAAGATTTCCATGGCACAGTTAGTTCAGCCCATTGCAAATGGAGCCTTTGTGTTGAGTATAGACACTGATTTTGATGGCTGCATGAAGCTAATTAGGGAAGTTACAGCAGAATTGCCCATTTACTTAGCCAATTCCTTGAATAGTTTGAGGCTTGAAGGGCAAAAGACGGCTGCCATTGAAATTCTACAGCAGTTTGATTGGGCAGTACCTGAATGGGTGATCATTCCAGGAGGCAATCTTGGGAACATATATGCATTTTACAAAGGATTCAAGATGTGCCAAGAGCTTGGCCTTGTTGACAGGATTCCTAGGCTTGTTTGTGCACAAGCAGCCAATGCTAACCCTCTGTATTTACACTACAAATCTGGGTGGAAAGATTTCAAGGCTGTGAAAGCAAGTTCGACGTTCGCCTCGGCCATTCAGATTGGGGATCCTGTTTCCATAGATAGAGCTGTTTGTGCTCTACAGAACTCGAATGGCATCGTCGAGGAAGCGACAGAGGAGGAACTAATGGATGCAATGGCACAAGCTGACTCTACTGGGATGTTCATTTGCCCTCACACAGGGGTGGCACTAACGGCTTTGATCAAGCTGAGAAACCAAGGTGTCATCAGCCCAACCGATAGGACGGTGGTGGTGAGCACTGCTCATGGCCTGAAGTTTACACAGTCGAAAATCGACTATCACTCCAAGGAAATCCCAGACATGGCTTGCCAATTTGCTAACCCACCTGTTCAAGTGAAGGCTGATTATGGGAGTGTCATGGATGTACTTAAAGATTATTTACTACAAAAGTCTCCAATGTCTAAAGTGTAG

Coding sequence (CDS)

ATGGCTGCCTCTTCGCTGTTAAACCCCGCCATTTCTTTCCCCTCTTACAAGCCCAAGCTCCAAGCCAACCCAAAGTTCCGAAATCCCATTCTCAGAATCGGACGAGTATCCGCCACTTCCGATTCGTCTTCCTCCACGCCGACTAATGGCTCTCCGTCATTGTCGACCACCAAGAACCGCCGCTCTGCCGACGAGAACATCAAAGACGAAGCACGGCGCCATCGTGCAGCCTCCGGAGAAGACCGTAGCAACTTCTCGGCCAAGTACGTGCCATTTAACGCCGGCGCCGACTCGTCTGAGTGGTACTCGCTTGACGAGGTCGTTTACCGGAGCCGATCTGGTGGATTGCTCGACGTTCAGCACGACATGGAGGCCTTGAAGAAATTCGACGGCGCGTACTGGCGCAACCTCTTCGATTCACGCGTGGGGAAAACCACATGGCCTTATGGGTCCGGTGTGTGGAGCAAGAAGGAGTGGGTGTTGCCGGAGATTGACCCGGACGACATCGTCAGCGCCTTCGAAGGTAACTCGAACCTGTTTTGGGCTGAGAGATTTGGCAAACAGTTTCTGGGTATGAATGATTTATGGGTAAAACACTGTGGGATTAGCCACACTGGGAGTTTCAAGGATTTGGGCATGACTGTTTTGGTTAGCCAAGTGAACCGGCTGAGGAAATTGAAACGCCCCGTCGTCGGAGTCGGTTGTGCATCGACCGGAGACACATCGGCCGCGCTCTCAGCCTACTGCGCATCTGCAGGGATCCCTTCAATCGTCTTCCTCCCTGCAAACAAGATTTCCATGGCACAGTTAGTTCAGCCCATTGCAAATGGAGCCTTTGTGTTGAGTATAGACACTGATTTTGATGGCTGCATGAAGCTAATTAGGGAAGTTACAGCAGAATTGCCCATTTACTTAGCCAATTCCTTGAATAGTTTGAGGCTTGAAGGGCAAAAGACGGCTGCCATTGAAATTCTACAGCAGTTTGATTGGGCAGTACCTGAATGGGTGATCATTCCAGGAGGCAATCTTGGGAACATATATGCATTTTACAAAGGATTCAAGATGTGCCAAGAGCTTGGCCTTGTTGACAGGATTCCTAGGCTTGTTTGTGCACAAGCAGCCAATGCTAACCCTCTGTATTTACACTACAAATCTGGGTGGAAAGATTTCAAGGCTGTGAAAGCAAGTTCGACGTTCGCCTCGGCCATTCAGATTGGGGATCCTGTTTCCATAGATAGAGCTGTTTGTGCTCTACAGAACTCGAATGGCATCGTCGAGGAAGCGACAGAGGAGGAACTAATGGATGCAATGGCACAAGCTGACTCTACTGGGATGTTCATTTGCCCTCACACAGGGGTGGCACTAACGGCTTTGATCAAGCTGAGAAACCAAGGTGTCATCAGCCCAACCGATAGGACGGTGGTGGTGAGCACTGCTCATGGCCTGAAGTTTACACAGTCGAAAATCGACTATCACTCCAAGGAAATCCCAGACATGGCTTGCCAATTTGCTAACCCACCTGTTCAAGTGAAGGCTGATTATGGGAGTGTCATGGATGTACTTAAAGATTATTTACTACAAAAGTCTCCAATGTCTAAAGTGTAG

Protein sequence

MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV

Homology

[Close]

BLAST of Carg12976 vs. NCBI nr
Match: XP_022960888.1 (threonine synthase, chloroplastic [Cucurbita moschata] >KAG7023681.1 hypothetical protein SDJN02_14707, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1070.1 bits (2766), Expect = 6.2e-309
Identity = 534/534 (100.00%), Postives = 534/534 (100.00%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60
           MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 535
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 534

[Close]

BLAST of Carg12976 vs. NCBI nr
Match: KAG6590018.1 (hypothetical protein SDJN03_15441, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1067.4 bits (2759), Expect = 4.1e-308
Identity = 533/534 (99.81%), Postives = 533/534 (99.81%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60
           MAASSLLNPAISFPSYKPKLQANPKFRNPIL IGRVSATSDSSSSTPTNGSPSLSTTKNR
Sbjct: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNR 156

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 157 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 216

Query: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 217 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 276

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 277 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 336

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 337 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 396

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 397 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 456

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 457 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 516

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 517 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 576

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 535
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV
Sbjct: 577 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 630

[Close]

BLAST of Carg12976 vs. NCBI nr
Match: XP_022987673.1 (threonine synthase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1061.6 bits (2744), Expect = 2.2e-306
Identity = 530/534 (99.25%), Postives = 531/534 (99.44%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60
           MAASSLLNPAISFPSYKPKLQANP FRNPILRI R SATSDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 535
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQK+PMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 534

[Close]

BLAST of Carg12976 vs. NCBI nr
Match: XP_023515876.1 (threonine synthase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1060.1 bits (2740), Expect = 6.5e-306
Identity = 529/534 (99.06%), Postives = 531/534 (99.44%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60
           MAASSLLNPAISFPSYKPKLQANP FRNPILRI R SA+SDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 535
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQK+PMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 534

[Close]

BLAST of Carg12976 vs. NCBI nr
Match: XP_038878968.1 (threonine synthase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1028.1 bits (2657), Expect = 2.7e-296
Identity = 508/534 (95.13%), Postives = 520/534 (97.38%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60
           MA SSLLNPAISFPS KPKLQ NPKFRNP+LRI + S+TSDSSSS PTNGSP LST KNR
Sbjct: 1   MATSSLLNPAISFPSRKPKLQPNPKFRNPVLRIPKASSTSDSSSSPPTNGSPPLSTLKNR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADS EWYSLDE++YRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSPEWYSLDEIIYRSRSGGLLDVQ 120

Query: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAV ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL+KLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALVKLRNKGIIRPTDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 535
           GLKFTQSKIDYHSK+IPDMACQFANPPVQVKADYGSVMDVLKDYLL K+P S+V
Sbjct: 481 GLKFTQSKIDYHSKDIPDMACQFANPPVQVKADYGSVMDVLKDYLLNKAPKSQV 534

[Close]

BLAST of Carg12976 vs. ExPASy Swiss-Prot
Match: Q9MT28 (Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 1.1e-250
Identity = 438/531 (82.49%), Postives = 471/531 (88.70%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKL--QANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTK 60
           MAAS +L  +   P   P+L  Q+  K  N I     + AT+ +          ++S  K
Sbjct: 1   MAASCMLRSSFISPGL-PQLHHQSTSKPNNGIHFFTPIKATATND---------AISQQK 60

Query: 61  NRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLD 120
           +RR ADENI++EARRH ++      NFSA+YVPFNAG +S EWYSLDE+VYRSRSGGLLD
Sbjct: 61  HRRPADENIREEARRHCSS-----HNFSARYVPFNAGPNSDEWYSLDEIVYRSRSGGLLD 120

Query: 121 VQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSN 180
           VQHDM+ALKKFDG YWR+LFDSRVGKTTWPYGSGVWSKKEWVLPEID DDIVSAFEGNSN
Sbjct: 121 VQHDMDALKKFDGQYWRSLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSN 180

Query: 181 LFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCAST 240
           LFWAERFGKQFLGM DLWVKHCGISHTGSFKDLGMTVLVSQVNRLRK+ +PVVGVGCAST
Sbjct: 181 LFWAERFGKQFLGMTDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCAST 240

Query: 241 GDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVT 300
           GDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCM+LIREVT
Sbjct: 241 GDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMQLIREVT 300

Query: 301 AELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQE 360
           AELPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGF+MC+E
Sbjct: 301 AELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPEWVIVPGGNLGNIYAFYKGFQMCKE 360

Query: 361 LGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCAL 420
           LGLVDRIPRLVCAQAANANPLYLHYKSGWKDFK VKA++TFASAIQIGDPVSIDRAV AL
Sbjct: 361 LGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKPVKANTTFASAIQIGDPVSIDRAVFAL 420

Query: 421 QNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVST 480
           Q  NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL KLRN GVI+PTDRTVVVST
Sbjct: 421 QQCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNSGVIAPTDRTVVVST 480

Query: 481 AHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKS 530
           AHGLKFTQSKIDYHSKEI DM C+FANPPV+VKAD+GSVMDVLK YLL ++
Sbjct: 481 AHGLKFTQSKIDYHSKEIKDMECRFANPPVEVKADFGSVMDVLKSYLLSQN 516

[Close]

BLAST of Carg12976 vs. ExPASy Swiss-Prot
Match: Q9S7B5 (Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 3.5e-246
Identity = 431/528 (81.63%), Postives = 468/528 (88.64%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGR---VSATSDSSSSTPTNGSPSLSTT 60
           MA+S L N ++S  S  PK     + R+  L   R   +S T+D ++      +P  +  
Sbjct: 1   MASSCLFNASVS--SLNPKQDPIRRHRSTSLLRHRPVVISCTADGNNIK----APIETAV 60

Query: 61  KNRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLL 120
           K     ++NI+DEARR+R+ +    + FSAKYVPFNA   S+E YSLDE+VYRSRSGGLL
Sbjct: 61  KPPHRTEDNIRDEARRNRSNA---VNPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLL 120

Query: 121 DVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNS 180
           DV+HDMEALK+FDGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID DDIVSAFEGNS
Sbjct: 121 DVEHDMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNS 180

Query: 181 NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCAS 240
           NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRK+KRPVVGVGCAS
Sbjct: 181 NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCAS 240

Query: 241 TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREV 300
           TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIRE+
Sbjct: 241 TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREI 300

Query: 301 TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQ 360
           TAELPIYLANSLNSLRLEGQKTAAIEILQQFDW VP+WVI+PGGNLGNIYAFYKGFKMCQ
Sbjct: 301 TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQ 360

Query: 361 ELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCA 420
           ELGLVDRIPR+VCAQAANANPLYLHYKSGWKDFK + AS+TFASAIQIGDPVSIDRAV A
Sbjct: 361 ELGLVDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYA 420

Query: 421 LQNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVS 480
           L+  NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL KLRNQGVI+PTDRTVVVS
Sbjct: 421 LKKCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVS 480

Query: 481 TAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYL 526
           TAHGLKFTQSKIDYHS  IPDMAC+F+NPPV VKAD+G+VMDVLK YL
Sbjct: 481 TAHGLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

[Close]

BLAST of Carg12976 vs. ExPASy Swiss-Prot
Match: Q9SSP5 (Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 6.0e-230
Identity = 409/526 (77.76%), Postives = 446/526 (84.79%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60
           MA+ SL + A  FPS+             +      S T   +S++P    P  +  K R
Sbjct: 1   MASFSLPHSATYFPSHS---------ETSLKPHSAASFTVRCTSASP--AVPPQTPQKPR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120
           RS DENI+DEARR          N SA+YVPFNA   S+E YSLDE+VYRS+SG LLDVQ
Sbjct: 61  RSPDENIRDEARRR----PHQLQNLSARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQ 120

Query: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HD  ALK++DG +WRNLFDSRVGKT WPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLF
Sbjct: 121 HDFAALKRYDGEFWRNLFDSRVGKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQ+L MNDLWVKHCGISHTGSFKDLGM+VLVSQVNRLRK+ +PV+GVGCASTGD
Sbjct: 181 WAERFGKQYLQMNDLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPA+KISMAQLVQPIANGAFVLSIDTDFDGCM LIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPADKISMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQF+W VP+WVI+PGGNLGNIYAFYKGF MC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWK-DFKAVKASSTFASAIQIGDPVSIDRAVCALQ 420
           LVDRIPRLVCAQAANANPLYLHYKSG+K DF  +KA++TFASAIQIGDPVSIDRAV AL+
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALK 420

Query: 421 NSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTA 480
            SNGIVEEATEEELMDA A ADSTGMFICPHTGVALTAL+KLR  GVI   DRTVVVSTA
Sbjct: 421 KSNGIVEEATEEELMDATALADSTGMFICPHTGVALTALMKLRKSGVIGANDRTVVVSTA 480

Query: 481 HGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYL 526
           HGLKFTQSKIDYHSK I +MAC+ ANPPV+VKA +GSVMDVLK+YL
Sbjct: 481 HGLKFTQSKIDYHSKNIKEMACRLANPPVKVKAKFGSVMDVLKEYL 511

[Close]

BLAST of Carg12976 vs. ExPASy Swiss-Prot
Match: Q58860 (Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=thrC PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.2e-72
Identity = 157/423 (37.12%), Postives = 242/423 (57.21%), Query Frame = 0


Query: 102 YSLDEVVYRSRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVL 161
           Y +DE++Y    GGLL++ +D E +K         + + ++ K       GVW   E+ L
Sbjct: 12  YDVDEIIYTCECGGLLEIIYDYEEIKD-------KVSEEKLRKRE----IGVWRYLEY-L 71

Query: 162 PEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVN 221
           P  D   IVS  EG + L+      K+ LG+ +L+VK+ G + TGSFKD GMTV V++ N
Sbjct: 72  PVKDESKIVSLCEGGTPLYRCNNLEKE-LGIKELYVKNEGANPTGSFKDRGMTVGVTRAN 131

Query: 222 RLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVL 281
            L      V  VGCASTG+TSA+L+AY A +G   IV LP  K+++ +L Q +  GA V+
Sbjct: 132 EL-----GVEVVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVI 191

Query: 282 SIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGG 341
            +  +FD  + +++++  E  IYL NS+N  RLEGQKT A EI  Q +W VP+ VI+P G
Sbjct: 192 QVKGNFDDALDMVKQLAKEKLIYLLNSINPFRLEGQKTIAFEICDQLNWQVPDRVIVPVG 251

Query: 342 NLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFAS 401
           N GNI A +KGFK  +  G++D +P++   QA  A P+   ++   KD    K   T A+
Sbjct: 252 NAGNISAIWKGFKEFEITGIIDELPKMTGIQADGAKPIVEAFRKRAKDIIPYKNPETIAT 311

Query: 402 AIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAM-AQADSTGMFICPHTGVALTALIK 461
           AI+IG+PV+  +A+ A+ +S G  E  T+EE+++A    A   G+F+ P +  ++  L K
Sbjct: 312 AIRIGNPVNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKK 371

Query: 462 LRNQGVISPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDV 521
           L  +G+I   +R V ++T HGLK             PD A + +  P++++ D   +  +
Sbjct: 372 LLEEGIIDRDERIVCITTGHGLK------------DPDAAIRASEEPIKIECDMNVLKRI 404

Query: 522 LKD 524
           LK+
Sbjct: 432 LKE 404

[Close]

BLAST of Carg12976 vs. ExPASy Swiss-Prot
Match: Q8TQD4 (Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=thrC PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 8.0e-65
Identity = 152/391 (38.87%), Postives = 227/391 (58.06%), Query Frame = 0


Query: 102 YSLDEVVYR-SRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWV 161
           YS DEV+Y  S+  GLLDV +D  ++K           D    KT  P    VW   + +
Sbjct: 13  YSRDEVIYTCSKCDGLLDVIYDYSSIK----------IDMEKLKTECP---SVWKYAKLL 72

Query: 162 LPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQV 221
             E +P   V+  EG + L+  +R  ++ +G+  L+VKH G++ TGSFKD GMTV V+  
Sbjct: 73  PVEREP---VTIQEGGTPLYKCDRLAEK-IGIKKLYVKHEGMNPTGSFKDRGMTVGVT-- 132

Query: 222 NRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFV 281
              + L+  +  V CASTG+TSAAL+ Y A AGIP +V LPA K+++ ++ Q + +GA V
Sbjct: 133 ---KALELGMNTVACASTGNTSAALAIYGAKAGIPVVVLLPAGKVALGKVAQALMHGAKV 192

Query: 282 LSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPG 341
           LSI  +FD  + L+R + ++  IYL NS+N  RLEGQKT   EI  Q D+ VP+ +++P 
Sbjct: 193 LSIRGNFDDALALVRTLCSQEKIYLLNSINPYRLEGQKTIGFEIADQLDFKVPDRIVLPV 252

Query: 342 GNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFA 401
           GN GNI A YKGF+  + LG+ D +P++   QA  + P+    KSG       +   T A
Sbjct: 253 GNAGNITAIYKGFREFKILGITDSLPKMTGIQAEGSCPIVKAIKSGAPAITPEENPETVA 312

Query: 402 SAIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAMAQ-ADSTGMFICPHTGVALTALI 461
           +AI+IG+PV+  +A+ A++ S G  E  T+EE++ A    A   G+ + P +  ++  L 
Sbjct: 313 TAIRIGNPVNATKALSAIRESGGTAESVTDEEILAAQKDLARLEGIGVEPASAASVAGLR 372

Query: 462 KLRNQGVISPTDRTVVVSTAHGLKFTQSKID 491
           KL + GVI   +  V ++T H LK  Q+ ID
Sbjct: 373 KLVDMGVIGRDETVVCITTGHLLKDPQTVID 381

[Close]

BLAST of Carg12976 vs. ExPASy TrEMBL
Match: A0A6J1HAA4 (Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111461551 PE=3 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 3.0e-309
Identity = 534/534 (100.00%), Postives = 534/534 (100.00%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60
           MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 535
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 534

[Close]

BLAST of Carg12976 vs. ExPASy TrEMBL
Match: A0A6J1JK49 (Threonine synthase OS=Cucurbita maxima OX=3661 GN=LOC111485156 PE=3 SV=1)

HSP 1 Score: 1061.6 bits (2744), Expect = 1.1e-306
Identity = 530/534 (99.25%), Postives = 531/534 (99.44%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60
           MAASSLLNPAISFPSYKPKLQANP FRNPILRI R SATSDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 535
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQK+PMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 534

[Close]

BLAST of Carg12976 vs. ExPASy TrEMBL
Match: A0A5D3CDZ3 (Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001840 PE=3 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 3.1e-290
Identity = 501/534 (93.82%), Postives = 513/534 (96.07%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60
           MA SSL NP ISFPS  PKLQ+ PKFRNPILRI +  ATSDSS S PTNGSP LST KNR
Sbjct: 1   MATSSLFNPTISFPSRNPKLQSTPKFRNPILRIPK--ATSDSSPSPPTNGSPPLSTLKNR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAG DS+E YSLDE+VYRSRSGGLLD+Q
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQ 120

Query: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKK+DGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRA+ ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAIYALQN 420

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 535
           GLKF QSKIDYHSKEIPDMACQFANPPVQVKAD+GSVMDVLKDYLL K+P SKV
Sbjct: 481 GLKFAQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNKAPKSKV 532

[Close]

BLAST of Carg12976 vs. ExPASy TrEMBL
Match: A0A5A7UKY2 (Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001890 PE=3 SV=1)

HSP 1 Score: 1005.0 bits (2597), Expect = 1.2e-289
Identity = 501/534 (93.82%), Postives = 512/534 (95.88%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60
           MA  SL NP ISFPS  PKLQ+ PKFRNPILRI +  ATSDSS S PTNGSP LST KNR
Sbjct: 1   MATYSLFNPTISFPSRNPKLQSTPKFRNPILRIPK--ATSDSSPSPPTNGSPPLSTLKNR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAG DS+E YSLDE+VYRSRSGGLLD+Q
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQ 120

Query: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKK+DGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAV ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 535
           GLKF QSKIDYHSKEIPDMACQFANPPVQVKAD+GSVMDVLKDYLL K+P SKV
Sbjct: 481 GLKFAQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNKAPKSKV 532

[Close]

BLAST of Carg12976 vs. ExPASy TrEMBL
Match: A0A0A0M223 (Threonine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G590270 PE=3 SV=1)

HSP 1 Score: 1005.0 bits (2597), Expect = 1.2e-289
Identity = 501/534 (93.82%), Postives = 512/534 (95.88%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60
           MA SSL NP IS PS  PKLQ+ PKFRNPILRI +  ATSDSS S PTNGSP LST KNR
Sbjct: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPK--ATSDSSPSPPTNGSPPLSTLKNR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAG DS+E YSLDE+VYRSRSGGLLD+Q
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQ 120

Query: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKK+DGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAV ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 535
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKAD+GSVMDVLKDYLL  +P SKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 532

[Close]

BLAST of Carg12976 vs. TAIR 10
Match: AT4G29840.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 852.0 bits (2200), Expect = 2.5e-247
Identity = 431/528 (81.63%), Postives = 468/528 (88.64%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGR---VSATSDSSSSTPTNGSPSLSTT 60
           MA+S L N ++S  S  PK     + R+  L   R   +S T+D ++      +P  +  
Sbjct: 1   MASSCLFNASVS--SLNPKQDPIRRHRSTSLLRHRPVVISCTADGNNIK----APIETAV 60

Query: 61  KNRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLL 120
           K     ++NI+DEARR+R+ +    + FSAKYVPFNA   S+E YSLDE+VYRSRSGGLL
Sbjct: 61  KPPHRTEDNIRDEARRNRSNA---VNPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLL 120

Query: 121 DVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNS 180
           DV+HDMEALK+FDGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID DDIVSAFEGNS
Sbjct: 121 DVEHDMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNS 180

Query: 181 NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCAS 240
           NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRK+KRPVVGVGCAS
Sbjct: 181 NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCAS 240

Query: 241 TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREV 300
           TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIRE+
Sbjct: 241 TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREI 300

Query: 301 TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQ 360
           TAELPIYLANSLNSLRLEGQKTAAIEILQQFDW VP+WVI+PGGNLGNIYAFYKGFKMCQ
Sbjct: 301 TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQ 360

Query: 361 ELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCA 420
           ELGLVDRIPR+VCAQAANANPLYLHYKSGWKDFK + AS+TFASAIQIGDPVSIDRAV A
Sbjct: 361 ELGLVDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYA 420

Query: 421 LQNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVS 480
           L+  NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL KLRNQGVI+PTDRTVVVS
Sbjct: 421 LKKCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVS 480

Query: 481 TAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYL 526
           TAHGLKFTQSKIDYHS  IPDMAC+F+NPPV VKAD+G+VMDVLK YL
Sbjct: 481 TAHGLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

[Close]

BLAST of Carg12976 vs. TAIR 10
Match: AT1G72810.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 798.1 bits (2060), Expect = 4.3e-231
Identity = 409/526 (77.76%), Postives = 446/526 (84.79%), Query Frame = 0


Query: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60
           MA+ SL + A  FPS+             +      S T   +S++P    P  +  K R
Sbjct: 1   MASFSLPHSATYFPSHS---------ETSLKPHSAASFTVRCTSASP--AVPPQTPQKPR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120
           RS DENI+DEARR          N SA+YVPFNA   S+E YSLDE+VYRS+SG LLDVQ
Sbjct: 61  RSPDENIRDEARRR----PHQLQNLSARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQ 120

Query: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HD  ALK++DG +WRNLFDSRVGKT WPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLF
Sbjct: 121 HDFAALKRYDGEFWRNLFDSRVGKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQ+L MNDLWVKHCGISHTGSFKDLGM+VLVSQVNRLRK+ +PV+GVGCASTGD
Sbjct: 181 WAERFGKQYLQMNDLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPA+KISMAQLVQPIANGAFVLSIDTDFDGCM LIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPADKISMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQF+W VP+WVI+PGGNLGNIYAFYKGF MC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWK-DFKAVKASSTFASAIQIGDPVSIDRAVCALQ 420
           LVDRIPRLVCAQAANANPLYLHYKSG+K DF  +KA++TFASAIQIGDPVSIDRAV AL+
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALK 420

Query: 421 NSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTA 480
            SNGIVEEATEEELMDA A ADSTGMFICPHTGVALTAL+KLR  GVI   DRTVVVSTA
Sbjct: 421 KSNGIVEEATEEELMDATALADSTGMFICPHTGVALTALMKLRKSGVIGANDRTVVVSTA 480

Query: 481 HGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYL 526
           HGLKFTQSKIDYHSK I +MAC+ ANPPV+VKA +GSVMDVLK+YL
Sbjct: 481 HGLKFTQSKIDYHSKNIKEMACRLANPPVKVKAKFGSVMDVLKEYL 511

The following BLAST results are available for this feature:

BLAST of Carg12976 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022960888.1	6.2e-309	100.00	threonine synthase, chloroplastic [Cucurbita moschata] >KAG7023681.1 hypothetica...	[more]
KAG6590018.1	4.1e-308	99.81	hypothetical protein SDJN03_15441, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022987673.1	2.2e-306	99.25	threonine synthase, chloroplastic [Cucurbita maxima]	[more]
XP_023515876.1	6.5e-306	99.06	threonine synthase, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_038878968.1	2.7e-296	95.13	threonine synthase, chloroplastic-like [Benincasa hispida]	[more]

BLAST of Carg12976 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MT28	1.1e-250	82.49	Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q9S7B5	3.5e-246	81.63	Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 ...	[more]
Q9SSP5	6.0e-230	77.76	Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 ...	[more]
Q58860	5.2e-72	37.12	Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 266...	[more]
Q8TQD4	8.0e-65	38.87	Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 /...	[more]

BLAST of Carg12976 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HAA4	3.0e-309	100.00	Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111461551 PE=3 SV=1	[more]
A0A6J1JK49	1.1e-306	99.25	Threonine synthase OS=Cucurbita maxima OX=3661 GN=LOC111485156 PE=3 SV=1	[more]
A0A5D3CDZ3	3.1e-290	93.82	Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00...	[more]
A0A5A7UKY2	1.2e-289	93.82	Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G0...	[more]
A0A0A0M223	1.2e-289	93.82	Threonine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G590270 PE=3 SV=1	[more]

BLAST of Carg12976 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29840.1	2.5e-247	81.63	Pyridoxal-5'-phosphate-dependent enzyme family protein	[more]
AT1G72810.1	4.3e-231	77.76	Pyridoxal-5'-phosphate-dependent enzyme family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004450	Threonine synthase-like	TIGRFAM	TIGR00260	TIGR00260	coord: 160..483 e-value: 1.3E-60 score: 203.3
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 61..283 e-value: 5.2E-64 score: 218.5
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 284..531 e-value: 1.0E-70 score: 240.5
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 84..524
IPR001926	Pyridoxal-phosphate dependent enzyme	PFAM	PF00291	PALP	coord: 172..478 e-value: 1.1E-47 score: 162.8
None	No IPR available	PIRSR	PIRSR038945-1	PIRSR038945-1	coord: 152..466 e-value: 1.7E-70 score: 235.6
None	No IPR available	PIRSR	PIRSR038945-2	PIRSR038945-2	coord: 152..466 e-value: 1.7E-70 score: 235.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 34..80
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 61..80
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 35..60
None	No IPR available	PANTHER	PTHR10314	CYSTATHIONINE BETA-SYNTHASE	coord: 31..528
None	No IPR available	PANTHER	PTHR10314:SF176	THREONINE SYNTHASE, CHLOROPLASTIC-LIKE ISOFORM X1	coord: 31..528
None	No IPR available	CDD	cd01563	Thr-synth_1	coord: 154..484 e-value: 1.31177E-158 score: 452.818
IPR000634	Serine/threonine dehydratase, pyridoxal-phosphate-binding site	PROSITE	PS00165	DEHYDRATASE_SER_THR	coord: 199..213

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12976-RA	Carg12976-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019344	cysteine biosynthetic process
biological_process	GO:0009088	threonine biosynthetic process
biological_process	GO:0006520	cellular amino acid metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0004795	threonine synthase activity