Carg12971 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12971
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type E3 ubiquitin transferase
Location	Carg_Chr10: 3479810 .. 3480361 (-)
RNA-Seq Expression	Carg12971
Synteny	Carg12971

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTGATTCTGCTTCAAATTATTTTGCTCAACCAACGTCTCATCCAGCAAAATCAAGCCTACCCATGTTGTATTACGGCCTTGTGGTAATCGGAACGGCGGCGATTGTGTTAGTTCTTTACAATTTCATCGTTATCATGTGGTGTTCCGACAACCGTCGTGGCCGAGCTTCGCCGGCGCTGGCTGGAAGTTATGCGGAGATGGTGAATTCAAGCCGTAGCTTGCAGAGCTCAAATAGGAATTTGTTGTGCAGCTTTAAGTTCAAGAAGGGGTCTGCAGCGGCGGCGACGGCGGCGGCCGAAGAAGAAGGGAATGGGAATGAATGTGTGGTTTGTTTGTCGGCGTTTGAGGAGGGGGAAGAGGTTAAGAAACTGCCGAGATGTATGCATATGTTTCATGCTTGTTGTATAGATATGTGGCTTTTTTCTCACTGTGATTGCCCCCTCTGCCGTGCTCCGGTGGCGGTGCCGGGGTTGAGCAAACGCCAAACCGCGGCGGAGCAACAGGGGAATTCAGGGCATGGGTTGCCGGAATCCGGTGGCTTGGTTTGA

mRNA sequence

ATGAGTGATTCTGCTTCAAATTATTTTGCTCAACCAACGTCTCATCCAGCAAAATCAAGCCTACCCATGTTGTATTACGGCCTTGTGGTAATCGGAACGGCGGCGATTGTGTTAGTTCTTTACAATTTCATCGTTATCATGTGGTGTTCCGACAACCGTCGTGGCCGAGCTTCGCCGGCGCTGGCTGGAAGTTATGCGGAGATGGTGAATTCAAGCCGTAGCTTGCAGAGCTCAAATAGGAATTTGTTGTGCAGCTTTAAGTTCAAGAAGGGGTCTGCAGCGGCGGCGACGGCGGCGGCCGAAGAAGAAGGGAATGGGAATGAATGTGTGGTTTGTTTGTCGGCGTTTGAGGAGGGGGAAGAGGTTAAGAAACTGCCGAGATGTATGCATATGTTTCATGCTTGTTGTATAGATATGTGGCTTTTTTCTCACTGTGATTGCCCCCTCTGCCGTGCTCCGGTGGCGGTGCCGGGGTTGAGCAAACGCCAAACCGCGGCGGAGCAACAGGGGAATTCAGGGCATGGGTTGCCGGAATCCGGTGGCTTGGTTTGA

Coding sequence (CDS)

ATGAGTGATTCTGCTTCAAATTATTTTGCTCAACCAACGTCTCATCCAGCAAAATCAAGCCTACCCATGTTGTATTACGGCCTTGTGGTAATCGGAACGGCGGCGATTGTGTTAGTTCTTTACAATTTCATCGTTATCATGTGGTGTTCCGACAACCGTCGTGGCCGAGCTTCGCCGGCGCTGGCTGGAAGTTATGCGGAGATGGTGAATTCAAGCCGTAGCTTGCAGAGCTCAAATAGGAATTTGTTGTGCAGCTTTAAGTTCAAGAAGGGGTCTGCAGCGGCGGCGACGGCGGCGGCCGAAGAAGAAGGGAATGGGAATGAATGTGTGGTTTGTTTGTCGGCGTTTGAGGAGGGGGAAGAGGTTAAGAAACTGCCGAGATGTATGCATATGTTTCATGCTTGTTGTATAGATATGTGGCTTTTTTCTCACTGTGATTGCCCCCTCTGCCGTGCTCCGGTGGCGGTGCCGGGGTTGAGCAAACGCCAAACCGCGGCGGAGCAACAGGGGAATTCAGGGCATGGGTTGCCGGAATCCGGTGGCTTGGTTTGA

Protein sequence

MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPALAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGEEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESGGLV

Homology

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BLAST of Carg12971 vs. NCBI nr
Match: KAG6590024.1 (RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023686.1 RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 368.6 bits (945), Expect = 3.1e-98
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60
           MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA
Sbjct: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGE 120
           LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGE
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGE 120

Query: 121 EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180
           EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG
Sbjct: 121 EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180

Query: 181 GLV 184
           GLV
Sbjct: 181 GLV 183

[Close]

BLAST of Carg12971 vs. NCBI nr
Match: XP_022960602.1 (E3 ubiquitin-protein ligase EL5-like [Cucurbita moschata])

HSP 1 Score: 360.1 bits (923), Expect = 1.1e-95
Identity = 181/183 (98.91%), Postives = 181/183 (98.91%), Query Frame = 0


Query: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60
           MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA
Sbjct: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGE 120
           LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAA  AAAEEEGNGNECVVCLSAFEEGE
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAA--AAAEEEGNGNECVVCLSAFEEGE 120

Query: 121 EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180
           EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG
Sbjct: 121 EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180

Query: 181 GLV 184
           GLV
Sbjct: 181 GLV 181

[Close]

BLAST of Carg12971 vs. NCBI nr
Match: XP_023516728.1 (E3 ubiquitin-protein ligase EL5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 352.1 bits (902), Expect = 3.0e-93
Identity = 177/184 (96.20%), Postives = 179/184 (97.28%), Query Frame = 0


Query: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60
           MSDSASNYFAQPT+HPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA
Sbjct: 1   MSDSASNYFAQPTNHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAA-AEEEGNGNECVVCLSAFEEG 120
           LAG+YAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAA AA  EEEGNGNECVVCLSAFEEG
Sbjct: 61  LAGNYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAAAAAVVEEEGNGNECVVCLSAFEEG 120

Query: 121 EEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPES 180
           EEVKKLPRCMHMFHA CIDMWLFSHCDCPLCRAPVAVPGLSKRQT AEQQGNSGHGLPES
Sbjct: 121 EEVKKLPRCMHMFHARCIDMWLFSHCDCPLCRAPVAVPGLSKRQTVAEQQGNSGHGLPES 180

Query: 181 GGLV 184
           GGLV
Sbjct: 181 GGLV 184

[Close]

BLAST of Carg12971 vs. NCBI nr
Match: XP_022987479.1 (E3 ubiquitin-protein ligase EL5-like [Cucurbita maxima])

HSP 1 Score: 347.1 bits (889), Expect = 9.6e-92
Identity = 174/183 (95.08%), Postives = 178/183 (97.27%), Query Frame = 0


Query: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60
           MSDSASN+F+QPT+HPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA
Sbjct: 1   MSDSASNHFSQPTNHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGE 120
           LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAA   AA EEEGNGNECVVCLSAFEEGE
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAA---AAVEEEGNGNECVVCLSAFEEGE 120

Query: 121 EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180
           EVKKLPRC+HMFHA CIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG
Sbjct: 121 EVKKLPRCLHMFHARCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180

Query: 181 GLV 184
           GLV
Sbjct: 181 GLV 180

[Close]

BLAST of Carg12971 vs. NCBI nr
Match: XP_022968369.1 (RING-H2 finger protein ATL52-like [Cucurbita maxima])

HSP 1 Score: 288.1 bits (736), Expect = 5.3e-74
Identity = 148/186 (79.57%), Postives = 154/186 (82.80%), Query Frame = 0


Query: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60
           M DSAS + AQPTSHPAKSSLPMLYYGLVVIGTA IVLVLYN I+I WCSDN  G+ASPA
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDNSHGQASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGE 120
           LAGSYAEMVNSSRS QSSNRNLL SFKFKKGS A ATA  EEEG GNEC VCLSAFE+GE
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTAKATATIEEEGIGNECAVCLSAFEDGE 120

Query: 121 EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQT---AAEQQGNSGHGLP 180
           EVKKLPRC HMFHA CIDMWL+SH DCPLCRAP   P L +R T   AAEQQ NSGHGL 
Sbjct: 121 EVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPAMAPALCQRHTAEDAAEQQVNSGHGLL 180

Query: 181 ESGGLV 184
           ES  LV
Sbjct: 181 ESSSLV 186

[Close]

BLAST of Carg12971 vs. ExPASy Swiss-Prot
Match: Q9LRB7 (E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5.1 PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.0e-16
Identity = 60/172 (34.88%), Postives = 83/172 (48.26%), Query Frame = 0


Query: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIV---LVLYNFIVIMWCSDNRRGRA 60
           M +S+S+    P S PA     M   G+  +    IV   L L   ++  +C + RR   
Sbjct: 12  MDESSSSSSPSPVSAPA-GQAAMTAGGIATVAAVLIVFAALTLAFVLLQCYCDERRRAVT 71

Query: 61  SPALAGSYAEMVNSSRSLQSSN------------RNLLCSFKFKKGSAAAATAAAEEEGN 120
           + + +G         RS    +            R+L  +   +  +AAAA    EE+ +
Sbjct: 72  TTSTSGRGRRPRPRRRSGSGGDGGTGGGVDPEVLRSLPVTVYSRSTAAAAAKEEEEEDDD 131

Query: 121 GNECVVCLSAFEEGEEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVP 158
           G EC VCL+  E+GEE + LPRC H FHA C+DMWL SH  CPLCR  V VP
Sbjct: 132 GVECAVCLAELEDGEEARFLPRCGHGFHAECVDMWLGSHSTCPLCRLTVVVP 182

[Close]

BLAST of Carg12971 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.0e-15
Identity = 59/184 (32.07%), Postives = 88/184 (47.83%), Query Frame = 0


Query: 7   NYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPAL----- 66
           ++F    S  + SS   L   L+ I T+A++LV Y  ++  +C  + +  +S  L     
Sbjct: 40  SFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHN 99

Query: 67  -AGSYAEMVNSSRSLQSSNRNLLCS---FKFKKGSAAAATAAAEEEGNGNECVVCLSAFE 126
             G ++     S +    N +++ S   +K+K G             +G++C VCLS FE
Sbjct: 100 GEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFV---------DGSDCSVCLSEFE 159

Query: 127 EGEEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRA-------PVAVPGLSKRQTAAEQQG 175
           E E ++ LP+C H FH  CID WL SH +CPLCRA       P A  G +     A  Q 
Sbjct: 160 ENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFVTGVNNPTASVGQNVSVVVA-NQS 213

[Close]

BLAST of Carg12971 vs. ExPASy Swiss-Prot
Match: P93823 (RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.4e-13
Identity = 52/154 (33.77%), Postives = 79/154 (51.30%), Query Frame = 0


Query: 12  PTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSD-------NRRGRAS---PAL 71
           P +  + ++ P+L   ++ I   A +LV Y   VI  C +        RR R+S   P +
Sbjct: 32  PRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQIDIFRRRRRSSDQNPLM 91

Query: 72  AGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGEE 131
             S  E+   +R L  S    +  FKFKK    A     ++  N  EC VCL+ F+E E+
Sbjct: 92  IYSPHEV---NRGLDESAIRAIPVFKFKKRDVVA--GEEDQSKNSQECSVCLNEFQEDEK 151

Query: 132 VKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVA 156
           ++ +P C H+FH  CID+WL  + +CPLCR  V+
Sbjct: 152 LRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVS 180

[Close]

BLAST of Carg12971 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-12
Identity = 57/166 (34.34%), Postives = 76/166 (45.78%), Query Frame = 0


Query: 12  PTSHPAKS---SLPMLYYGLVVIGTAAIVLVLYNFIVIMWC--------------SDNRR 71
           P  H A S   S P+L   ++ I   A +LV Y   VI  C              S  RR
Sbjct: 22  PIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSLSRRRR 81

Query: 72  GRASPALAGSYAEMVNSSRSLQSSNRNLLCSFKFKK-----GSAAAATAAAEEEGNGNEC 131
               P +  S  E+   SR L  S    +  FKFKK               EEE    EC
Sbjct: 82  NDQDPLMVYS-PEL--RSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQEC 141

Query: 132 VVCLSAFEEGEEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVA 156
            VCLS F++ E+++ +P C H+FH  CID+WL ++ +CPLCR  V+
Sbjct: 142 SVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVS 184

[Close]

BLAST of Carg12971 vs. ExPASy Swiss-Prot
Match: P0C041 (Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 PE=3 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 1.0e-11
Identity = 51/153 (33.33%), Postives = 73/153 (47.71%), Query Frame = 0


Query: 14  SHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPALAGSYAEM-VNSS 73
           S+P  S L +  +G   I   A +L  Y  +V  +C+++    A+     S   + VNS 
Sbjct: 54  SNPNLSPLVIAIFG---IFATAFLLAAYYTLVSKYCANDTTNEAASESGRSDIILDVNSP 113

Query: 74  R--------SLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGEEVKK 133
                    +L+SS   L  +   K G           + NG +C +CL  F E E ++ 
Sbjct: 114 ERGDQDDPFALESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRL 173

Query: 134 LPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVP 158
           LP+C H FH  CID WL SH +CPLCRA + VP
Sbjct: 174 LPKCNHTFHVVCIDRWLKSHSNCPLCRAKIIVP 203

[Close]

BLAST of Carg12971 vs. ExPASy TrEMBL
Match: A0A6J1H7W2 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461337 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 5.3e-96
Identity = 181/183 (98.91%), Postives = 181/183 (98.91%), Query Frame = 0


Query: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60
           MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA
Sbjct: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGE 120
           LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAA  AAAEEEGNGNECVVCLSAFEEGE
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAA--AAAEEEGNGNECVVCLSAFEEGE 120

Query: 121 EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180
           EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG
Sbjct: 121 EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180

Query: 181 GLV 184
           GLV
Sbjct: 181 GLV 181

[Close]

BLAST of Carg12971 vs. ExPASy TrEMBL
Match: A0A6J1JJ05 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485020 PE=3 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 4.6e-92
Identity = 174/183 (95.08%), Postives = 178/183 (97.27%), Query Frame = 0


Query: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60
           MSDSASN+F+QPT+HPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA
Sbjct: 1   MSDSASNHFSQPTNHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGE 120
           LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAA   AA EEEGNGNECVVCLSAFEEGE
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAA---AAVEEEGNGNECVVCLSAFEEGE 120

Query: 121 EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180
           EVKKLPRC+HMFHA CIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG
Sbjct: 121 EVKKLPRCLHMFHARCIDMWLFSHCDCPLCRAPVAVPGLSKRQTAAEQQGNSGHGLPESG 180

Query: 181 GLV 184
           GLV
Sbjct: 181 GLV 180

[Close]

BLAST of Carg12971 vs. ExPASy TrEMBL
Match: A0A6J1HZG2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467629 PE=3 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.6e-74
Identity = 148/186 (79.57%), Postives = 154/186 (82.80%), Query Frame = 0


Query: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60
           M DSAS + AQPTSHPAKSSLPMLYYGLVVIGTA IVLVLYN I+I WCSDN  G+ASPA
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDNSHGQASPA 60

Query: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGE 120
           LAGSYAEMVNSSRS QSSNRNLL SFKFKKGS A ATA  EEEG GNEC VCLSAFE+GE
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTAKATATIEEEGIGNECAVCLSAFEDGE 120

Query: 121 EVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQT---AAEQQGNSGHGLP 180
           EVKKLPRC HMFHA CIDMWL+SH DCPLCRAP   P L +R T   AAEQQ NSGHGL 
Sbjct: 121 EVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPAMAPALCQRHTAEDAAEQQVNSGHGLL 180

Query: 181 ESGGLV 184
           ES  LV
Sbjct: 181 ESSSLV 186

[Close]

BLAST of Carg12971 vs. ExPASy TrEMBL
Match: A0A6J1D0Z7 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016600 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.1e-68
Identity = 142/183 (77.60%), Postives = 155/183 (84.70%), Query Frame = 0


Query: 2   SDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPAL 61
           S S+S  F QPT HPAKSSLPMLYYGLVV+GTAAIVLV+YNFIVI WCSD RRGR SP +
Sbjct: 4   SYSSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNFIVIRWCSD-RRGRGSPGM 63

Query: 62  AGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGEE 121
           A SYAEM++SS S QSS+RNLL SF+FKKG+ AAA A AEEEGNG EC VCLSAFE+GEE
Sbjct: 64  ARSYAEMMSSSGSFQSSSRNLLSSFRFKKGTKAAA-ATAEEEGNGLECAVCLSAFEDGEE 123

Query: 122 VKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAV-PGLSKRQTAAEQQGNSGHGLPESG 181
           VKKLPRC HMFHA CIDMWL+SH DCPLCRAPVA+ PGL +  TAAEQ  NSGHGL ESG
Sbjct: 124 VKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAPGLCQHHTAAEQPENSGHGLLESG 183

Query: 182 GLV 184
           GLV
Sbjct: 184 GLV 184

[Close]

BLAST of Carg12971 vs. ExPASy TrEMBL
Match: A0A6J1EGS5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434176 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 6.7e-67
Identity = 134/167 (80.24%), Postives = 141/167 (84.43%), Query Frame = 0


Query: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60
           M DSAS + AQPTSHPAKSSLPMLYYGLVVIGTA IVLVLYN I+I WCSD+  G+AS A
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASTA 60

Query: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGS--AAAATAAAEEEGNGNECVVCLSAFEE 120
           LAGSYAEMVNSSRS QSSNRNLL SFKFKKGS   AAATA  EEEG GNEC VCLSAFE+
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120

Query: 121 GEEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTA 166
           GEEVKKLPRC HMFHA CIDMWL+SH DCPLCRAPV  P L +R TA
Sbjct: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTA 167

[Close]

BLAST of Carg12971 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 84.0 bits (206), Expect = 1.4e-16
Identity = 59/184 (32.07%), Postives = 88/184 (47.83%), Query Frame = 0


Query: 7   NYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPAL----- 66
           ++F    S  + SS   L   L+ I T+A++LV Y  ++  +C  + +  +S  L     
Sbjct: 40  SFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHN 99

Query: 67  -AGSYAEMVNSSRSLQSSNRNLLCS---FKFKKGSAAAATAAAEEEGNGNECVVCLSAFE 126
             G ++     S +    N +++ S   +K+K G             +G++C VCLS FE
Sbjct: 100 GEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFV---------DGSDCSVCLSEFE 159

Query: 127 EGEEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRA-------PVAVPGLSKRQTAAEQQG 175
           E E ++ LP+C H FH  CID WL SH +CPLCRA       P A  G +     A  Q 
Sbjct: 160 ENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFVTGVNNPTASVGQNVSVVVA-NQS 213

[Close]

BLAST of Carg12971 vs. TAIR 10
Match: AT1G04360.1 (RING/U-box superfamily protein )

HSP 1 Score: 77.8 bits (190), Expect = 1.0e-14
Identity = 52/154 (33.77%), Postives = 79/154 (51.30%), Query Frame = 0


Query: 12  PTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSD-------NRRGRAS---PAL 71
           P +  + ++ P+L   ++ I   A +LV Y   VI  C +        RR R+S   P +
Sbjct: 32  PRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQIDIFRRRRRSSDQNPLM 91

Query: 72  AGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGEE 131
             S  E+   +R L  S    +  FKFKK    A     ++  N  EC VCL+ F+E E+
Sbjct: 92  IYSPHEV---NRGLDESAIRAIPVFKFKKRDVVA--GEEDQSKNSQECSVCLNEFQEDEK 151

Query: 132 VKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVA 156
           ++ +P C H+FH  CID+WL  + +CPLCR  V+
Sbjct: 152 LRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVS 180

[Close]

BLAST of Carg12971 vs. TAIR 10
Match: AT4G33565.1 (RING/U-box superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.1e-13
Identity = 30/49 (61.22%), Postives = 36/49 (73.47%), Query Frame = 0


Query: 106 GNECVVCLSAFEEGEEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPV 155
           G +C VCLS FEE E ++ LP+C H FH  CID WL SH +CPLCRAP+
Sbjct: 214 GTDCSVCLSEFEEEETLRLLPKCKHAFHLYCIDTWLRSHTNCPLCRAPI 262

[Close]

BLAST of Carg12971 vs. TAIR 10
Match: AT5G43420.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.9 bits (180), Expect = 1.5e-13
Identity = 57/166 (34.34%), Postives = 76/166 (45.78%), Query Frame = 0


Query: 12  PTSHPAKS---SLPMLYYGLVVIGTAAIVLVLYNFIVIMWC--------------SDNRR 71
           P  H A S   S P+L   ++ I   A +LV Y   VI  C              S  RR
Sbjct: 22  PIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSLSRRRR 81

Query: 72  GRASPALAGSYAEMVNSSRSLQSSNRNLLCSFKFKK-----GSAAAATAAAEEEGNGNEC 131
               P +  S  E+   SR L  S    +  FKFKK               EEE    EC
Sbjct: 82  NDQDPLMVYS-PEL--RSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQEC 141

Query: 132 VVCLSAFEEGEEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVA 156
            VCLS F++ E+++ +P C H+FH  CID+WL ++ +CPLCR  V+
Sbjct: 142 SVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVS 184

[Close]

BLAST of Carg12971 vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 71.6 bits (174), Expect = 7.2e-13
Identity = 51/153 (33.33%), Postives = 73/153 (47.71%), Query Frame = 0


Query: 14  SHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPALAGSYAEM-VNSS 73
           S+P  S L +  +G   I   A +L  Y  +V  +C+++    A+     S   + VNS 
Sbjct: 54  SNPNLSPLVIAIFG---IFATAFLLAAYYTLVSKYCANDTTNEAASESGRSDIILDVNSP 113

Query: 74  R--------SLQSSNRNLLCSFKFKKGSAAAATAAAEEEGNGNECVVCLSAFEEGEEVKK 133
                    +L+SS   L  +   K G           + NG +C +CL  F E E ++ 
Sbjct: 114 ERGDQDDPFALESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRL 173

Query: 134 LPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVP 158
           LP+C H FH  CID WL SH +CPLCRA + VP
Sbjct: 174 LPKCNHTFHVVCIDRWLKSHSNCPLCRAKIIVP 203

The following BLAST results are available for this feature:

BLAST of Carg12971 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6590024.1	3.1e-98	100.00	RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. sororia] >KA...	[more]
XP_022960602.1	1.1e-95	98.91	E3 ubiquitin-protein ligase EL5-like [Cucurbita moschata]	[more]
XP_023516728.1	3.0e-93	96.20	E3 ubiquitin-protein ligase EL5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022987479.1	9.6e-92	95.08	E3 ubiquitin-protein ligase EL5-like [Cucurbita maxima]	[more]
XP_022968369.1	5.3e-74	79.57	RING-H2 finger protein ATL52-like [Cucurbita maxima]	[more]

BLAST of Carg12971 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LRB7	4.0e-16	34.88	E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5....	[more]
Q9LF64	2.0e-15	32.07	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]
P93823	1.4e-13	33.77	RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1	[more]
Q9LSW9	2.1e-12	34.34	RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1	[more]
P0C041	1.0e-11	33.33	Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 P...	[more]

BLAST of Carg12971 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H7W2	5.3e-96	98.91	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461337...	[more]
A0A6J1JJ05	4.6e-92	95.08	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485020 P...	[more]
A0A6J1HZG2	2.6e-74	79.57	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467629 P...	[more]
A0A6J1D0Z7	2.1e-68	77.60	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101660...	[more]
A0A6J1EGS5	6.7e-67	80.24	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434176...	[more]

BLAST of Carg12971 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17600.1	1.4e-16	32.07	RING/U-box superfamily protein	[more]
AT1G04360.1	1.0e-14	33.77	RING/U-box superfamily protein	[more]
AT4G33565.1	1.1e-13	61.22	RING/U-box superfamily protein	[more]
AT5G43420.1	1.5e-13	34.34	RING/U-box superfamily protein	[more]
AT4G17905.1	7.2e-13	33.33	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 109..150 e-value: 0.0027 score: 27.0
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 107..151 e-value: 1.9E-10 score: 40.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 109..151 score: 11.323791
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 65..156 e-value: 1.6E-18 score: 68.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 163..183
None	No IPR available	PANTHER	PTHR45676:SF84	RING-H2 FINGER PROTEIN ATL52-LIKE	coord: 7..177
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 102..157
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 7..177

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12971-RA	Carg12971-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane