Carg12953 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg12953 vs. NCBI nr
Match: XP_022960792.1 (subtilisin-like protease SBT1.8 [Cucurbita moschata] >KAG7023704.1 Subtilisin-like protease SBT1.8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1520.0 bits (3934), Expect = 0.0e+00
Identity = 766/766 (100.00%), Postives = 766/766 (100.00%), Query Frame = 0

Query: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
           MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660
           NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 767
           ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Carg12953 vs. NCBI nr
Match: KAG6590040.1 (Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1514.2 bits (3919), Expect = 0.0e+00
Identity = 765/766 (99.87%), Postives = 765/766 (99.87%), Query Frame = 0

Query: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
           MGSMARLLVASF LLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS
Sbjct: 1   MGSMARLLVASF-LLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660
           NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 767
           ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 765

BLAST of Carg12953 vs. NCBI nr
Match: XP_022987083.1 (subtilisin-like protease SBT1.8 [Cucurbita maxima])

HSP 1 Score: 1510.7 bits (3910), Expect = 0.0e+00
Identity = 761/766 (99.35%), Postives = 762/766 (99.48%), Query Frame = 0

Query: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
           MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVY LHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660
           NPWAHGAGHVDPHKALSPGL+YDISTNDYITFLCSLDYGIDHVQAI KRSNITC KKFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 767
           ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Carg12953 vs. NCBI nr
Match: XP_023516159.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1505.3 bits (3896), Expect = 0.0e+00
Identity = 761/766 (99.35%), Postives = 761/766 (99.35%), Query Frame = 0

Query: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
           MGSMA LLVA F LLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS
Sbjct: 1   MGSMASLLVAYF-LLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660
           NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITC KKFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCSKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 767
           ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 765

BLAST of Carg12953 vs. NCBI nr
Match: XP_038879224.1 (subtilisin-like protease SBT1.8 [Benincasa hispida])

HSP 1 Score: 1441.8 bits (3731), Expect = 0.0e+00
Identity = 724/766 (94.52%), Postives = 745/766 (97.26%), Query Frame = 0

Query: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
           MGSMARLL+A    LLLLLPCVFVNAK+TYIVRMKH+ALPS+YLTHHDWYSAHLQSLSSS
Sbjct: 1   MGSMARLLIA---FLLLLLPCVFVNAKKTYIVRMKHNALPSEYLTHHDWYSAHLQSLSSS 60

Query: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           +TSDSLLYTYTSAYHGFAASLD +EAELLRQSDSVLGVYED+VY LHTTRTPGFLGLDSD
Sbjct: 61  STSDSLLYTYTSAYHGFAASLDSNEAELLRQSDSVLGVYEDSVYTLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESG DFSPS
Sbjct: 121 FGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGADFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK RENESPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQ+GNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQIGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMG+K V LVYNKGSN+SSNMCLPGSLEPA VRGKVV+CDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGSKAVELVYNKGSNTSSNMCLPGSLEPAVVRGKVVVCDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRS + PTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
           GT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ
Sbjct: 481 GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660
           NPWAHGAGHVDPHKALSPGL+YDISTNDYI FLCSLDYGIDHVQAI KRSNITC +KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFTKVG 720

Query: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 767
           ERKRYTVTFVASRDAA+T R+GFGSIAW+N QHQVRSPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAAQTMRFGFGSIAWNNYQHQVRSPVAFAWTRL 763

BLAST of Carg12953 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 548/759 (72.20%), Postives = 632/759 (83.27%), Query Frame = 0

Query: 8   LVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSATSDSLL 67
           ++ +FL LLL        AK+TYI+R+ H   P  +LTHHDWY++ L S S      SLL
Sbjct: 13  IITTFLFLLL-----HTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLL 72

Query: 68  YTYTSAYHGFAASLDPDEAE-LLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEG 127
           YTYT+++HGF+A LD  EA+ LL  S+S+L ++ED +Y LHTTRTP FLGL+S+FG+   
Sbjct: 73  YTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV--- 132

Query: 128 HNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKL 187
               DL  +S+ VIIGVLDTG+WPES+SFDDT MPEIPS+W+GECESG DF   LCNKKL
Sbjct: 133 ---HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKL 192

Query: 188 IGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIA 247
           IGARSFSKG+QMASGGG F   RE+ SPRD DGHGTHT++TAAGS V NAS LGYA G A
Sbjct: 193 IGARSFSKGFQMASGGG-FSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTA 252

Query: 248 RGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGA 307
           RGMA +ARVA YK CW TGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYYRDTIAIGA
Sbjct: 253 RGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGA 312

Query: 308 FAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSL 367
           F+AME+GVFVSCSAGNSGP +AS+ANVAPW+MTVGAGTLDRDFPA+  LGNGKR TGVSL
Sbjct: 313 FSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSL 372

Query: 368 YSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGGVVRDA 427
           YSG GMG KP+ LVYNKG++SSSN+CLPGSL+ + VRGK+V+CDRG+NARVEKG VVRDA
Sbjct: 373 YSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDA 432

Query: 428 GGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGGTVLNV 487
           GG+GMI+ANTAASGEELVADSHLLPA+AVG KTGDL+R+YV+S +KPTA+L F GTVL+V
Sbjct: 433 GGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDV 492

Query: 488 RPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSG 547
           +PSPVVAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGLD D R+TQFNIMSG
Sbjct: 493 KPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSG 552

Query: 548 TSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHG 607
           TSMSCPHISGLA LLKAAHP WSPSAIKSALMTTAY  DNTN+ L DAA  + SNP+AHG
Sbjct: 553 TSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHG 612

Query: 608 AGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFADPGQLNY 667
           +GHVDP KALSPGLVYDIST +YI FLCSLDY +DH+ AI KR ++ C KKF+DPGQLNY
Sbjct: 613 SGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNY 672

Query: 668 PSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGERKRYT 727
           PSFSV+FG KRVVRYTR VTNVGAA SVY+V       V ++VKPSKL F  VGE+KRYT
Sbjct: 673 PSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYT 732

Query: 728 VTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTK 766
           VTFV+ +  + T +  FGSI WSN QH+VRSPVAF+W +
Sbjct: 733 VTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNR 753

BLAST of Carg12953 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 3.5e-231
Identity = 429/771 (55.64%), Postives = 546/771 (70.82%), Query Frame = 0

Query: 3   SMARLLVASFLLLLLLLPCVFVNA---KRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSS 62
           S + L   +F LLL L  C   ++   + TYIV M    +PS +  H +WY + L+S+S 
Sbjct: 2   SSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISD 61

Query: 63  SATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS 122
           SA    LLYTY +A HGF+  L  +EA+ L     V+ V  +  Y LHTTRTP FLGLD 
Sbjct: 62  SA---ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD- 121

Query: 123 DFGLWEGHNTQDL--DQASH-DVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPD 182
                   +T DL  +  S+ DV++GVLDTG+WPESKS+ D G   IPS W+G CE+G +
Sbjct: 122 -------EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTN 181

Query: 183 FSPSLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANA 242
           F+ SLCN+KLIGAR F++GY+  S  G   +S+E+ SPRD DGHGTHT+STAAGS V  A
Sbjct: 182 FTASLCNRKLIGARFFARGYE--STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGA 241

Query: 243 SLLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAP 302
           SLLGYA G ARGMAP+ARVA YK CW  GCF SDILA +D+AI+D V+VLS+SLGGG + 
Sbjct: 242 SLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSD 301

Query: 303 YYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLG 362
           YYRD +AIGAFAAME+G+ VSCSAGN+GP+ +SL+NVAPWI TVGAGTLDRDFPA   LG
Sbjct: 302 YYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILG 361

Query: 363 NGKRFTGVSLYSGQGMGNKPVALVY--NKGSNSSSNMCLPGSLEPAFVRGKVVICDRGIN 422
           NGK FTGVSL+ G+ + +K +  +Y  N  + ++ N+C+ G+L P  V+GK+V+CDRGIN
Sbjct: 362 NGKNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN 421

Query: 423 ARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPT 482
           ARV+KG VV+ AGG+GMILANTAA+GEELVAD+HLLPA  VG K GD+IR YV +   PT
Sbjct: 422 ARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT 481

Query: 483 AVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDN 542
           A +S  GTV+ V+PSPVVAAFSSRGPN +TP ILKPD+I PGVNILAAW+ + GPTGL +
Sbjct: 482 ASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLAS 541

Query: 543 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDA 602
           D R+ +FNI+SGTSMSCPH+SGLAALLK+ HP WSP+AI+SALMTTAY        L D 
Sbjct: 542 DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI 601

Query: 603 AGGAFSNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITC 662
           A G  S P+ HGAGHV P  A +PGL+YD++T DY+ FLC+L+Y    ++++++R N TC
Sbjct: 602 ATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRR-NYTC 661

Query: 663 -LKKFADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSK 722
              K      LNYPSF+V        +YTR VT+VG AG+     T+  + VK++V+P+ 
Sbjct: 662 DPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAV 721

Query: 723 LVFSKVGERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWT 765
           L F +  E+K YTVTF         +   FGSI WS+ +H V SPVA +WT
Sbjct: 722 LNFKEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Carg12953 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 1.2e-220
Identity = 416/777 (53.54%), Postives = 523/777 (67.31%), Query Frame = 0

Query: 4   MARLLVASFLLLLLLLPCVF------VNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSL 63
           MA+L ++S   +  LL C F       +   +YIV ++    PS + +H++W+ + L+SL
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL 60

Query: 64  SSSATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 123
            SS    +LLY+Y+ A HGF+A L P +   LR+  SV+ V  D    +HTT TP FLG 
Sbjct: 61  PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 120

Query: 124 DSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDF 183
             + GLW   N         DVI+GVLDTGIWPE  SF D+G+  IPS W+GECE GPDF
Sbjct: 121 SQNSGLWSNSN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDF 180

Query: 184 SPSLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANAS 243
             S CN+KLIGAR+F +GY     G     ++E+ SPRD +GHGTHTASTAAGS VANAS
Sbjct: 181 PASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANAS 240

Query: 244 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLG-GGSAP 303
           L  YARG A GMA +AR+AAYK CW  GC+ SDILA MD+A++DGV V+SLS+G  GSAP
Sbjct: 241 LYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAP 300

Query: 304 -YYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQL 363
            Y+ D+IAIGAF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    
Sbjct: 301 EYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAIT 360

Query: 364 GNGKRFTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINA 423
           G+GK FTG SLY+G+ + +  ++LVY+   +  S +C PG L  + V GK+V+CDRG NA
Sbjct: 361 GDGKVFTGTSLYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNA 420

Query: 424 RVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTA 483
           RVEKG  V+ AGG GMILANTA SGEEL ADSHL+PA  VGAK GD IR Y+++   PTA
Sbjct: 421 RVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTA 480

Query: 484 VLSFGGTVLNVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDN 543
            +SF GT++    PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  +GPT LD 
Sbjct: 481 KISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDI 540

Query: 544 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDA 603
           D R+ QFNI+SGTSMSCPH+SGLAALL+ AHP WSP+AIKSAL+TTAY  +N+   + D 
Sbjct: 541 DPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDL 600

Query: 604 AGGAFSNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNI-- 663
           A G  SN + HGAGHVDP+KAL+PGLVYDI   +Y+ FLC++ Y    +    +   +  
Sbjct: 601 ATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYD 660

Query: 664 TC-LKKFADPGQLNYPSFSVVFGSK-RVVRYTRIVTNVGA-AGSVYEVATTAPSVVKVTV 723
            C   K    G LNYPSFSVVF S   VV+Y R+V NVG+   +VYEV   +P+ V++ V
Sbjct: 661 ACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDV 720

Query: 724 KPSKLVFSKVGERKRYTVTF---VASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAW 764
            PSKL FSK      Y VTF   V          + FGSI W++ +H V+SPVA  W
Sbjct: 721 SPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Carg12953 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 3.5e-215
Identity = 396/775 (51.10%), Postives = 515/775 (66.45%), Query Frame = 0

Query: 12  FLLLLLLLPCVFVNA--------KRTYIVRMKHHALPSDYLTHHDWYSAHLQSLS----- 71
           FL ++L +  +F+ A        K+TY++ M   A+P  Y  H  WYS+ + S++     
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 72  -SSATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 131
                ++ +LYTY +A+HG AA L  +EAE L + D V+ V  +T Y LHTTR+P FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 132 DSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDF 191
           +      E           HDV++GVLDTGIWPES+SF+DTGM  +P+ WRG CE+G  F
Sbjct: 131 ERQ----ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRF 190

Query: 192 SPSLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANAS 251
               CN+K++GAR F +GY+ A+  G   +  E +SPRD+DGHGTHTA+T AGS V  A+
Sbjct: 191 LKRNCNRKIVGARVFYRGYEAAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGAN 250

Query: 252 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 311
           L G+A G ARGMA +ARVAAYK CW  GCF SDIL+ +D+A++DGV VLS+SLGGG + Y
Sbjct: 251 LFGFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTY 310

Query: 312 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 371
            RD+++I  F AME GVFVSCSAGN GP+  SL NV+PWI TVGA T+DRDFPA V++G 
Sbjct: 311 SRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGT 370

Query: 372 GKRFTGVSLYSGQGM--GNKPVALVY---NKGSNSSSNMCLPGSLEPAFVRGKVVICDRG 431
            + F GVSLY G+ +   NK   LVY   N  S   ++ CL G+L+   V GK+VICDRG
Sbjct: 371 MRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRG 430

Query: 432 INARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAK 491
           +  RV+KG VV+ AGGIGM+L NTA +GEELVADSH+LPAVAVG K G LI+QY  +  K
Sbjct: 431 VTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKK 490

Query: 492 PTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL 551
            TA L   GT + ++PSPVVAAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L
Sbjct: 491 ATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSL 550

Query: 552 DNDKRKTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLR 611
            +D R+ +FNI+SGTSMSCPH+SG+AAL+K+ HP WSP+AIKSALMTTAY  DN    L 
Sbjct: 551 SSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLT 610

Query: 612 DAAGGAFSNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNI 671
           DA+G A S+P+ HGAGH+DP +A  PGLVYDI   +Y  FLC+ D     ++   K SN 
Sbjct: 611 DASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNR 670

Query: 672 TCLKKFA-DPGQLNYPSFSVVFGSK---RVVRYTRIVTNVGAAGSVYEVATTAPSVVKVT 731
           TC    A +PG LNYP+ S +F      + +   R VTNVG   S Y+V+ +      VT
Sbjct: 671 TCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVT 730

Query: 732 VKPSKLVFSKVGERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAW 764
           V+P  L F+   ++  YTVTF   R   R  R  FG + W +  H+VRSPV   W
Sbjct: 731 VQPKTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Carg12953 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 4.9e-209
Identity = 392/770 (50.91%), Postives = 523/770 (67.92%), Query Frame = 0

Query: 8   LVASFLLLLLLLPCVFVN-----AKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSAT 67
           + +S ++LLL L   F++     A +T+I R+   ++PS + TH+ WYS         A 
Sbjct: 1   MASSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEF------AE 60

Query: 68  SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFG 127
              +++ Y + +HGF+A + PDEA+ LR   +VL V+ED    LHTTR+P FLGL +  G
Sbjct: 61  ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKG 120

Query: 128 LWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLC 187
           LW      + D  S DVIIGV DTGIWPE +SF D  +  IP RWRG CESG  FSP  C
Sbjct: 121 LW-----SESDYGS-DVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNC 180

Query: 188 NKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLGYA 247
           N+K+IGAR F+KG Q A  GG   K+ E  SPRD DGHGTHT+STAAG H   AS+ GYA
Sbjct: 181 NRKIIGARFFAKGQQAAVIGG-INKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYA 240

Query: 248 RGIARGMAPQARVAAYKTCW-PTGCFGSDILAGMDRAISDGVDVLSLSLGGG---SAPYY 307
            G+A+G+AP+AR+AAYK CW  +GC  SDILA  D A+ DGVDV+S+S+GGG   ++PYY
Sbjct: 241 SGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYY 300

Query: 308 RDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNG 367
            D IAIG++ A  KG+FVS SAGN GPN  S+ N+APW+ TVGA T+DR+FPA   LG+G
Sbjct: 301 LDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDG 360

Query: 368 KRFTGVSLYSGQGMGNKPVALVY-NKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARV 427
            R  GVSLY+G  +  +   +VY  K   SS+++C+  +L+P  VRGK+VICDRG + RV
Sbjct: 361 HRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRV 420

Query: 428 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVL 487
            KG VV+ AGG+GMILAN A++GE LV D+HL+PA AVG+  GD I+ Y  S   P A +
Sbjct: 421 AKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASI 480

Query: 488 SFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 547
            F GT++ ++P+PV+A+FS RGPN ++P+ILKPD+I PGVNILAAW++++GPTGL +D R
Sbjct: 481 DFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPR 540

Query: 548 KTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 607
           KT+FNI+SGTSM+CPH+SG AALLK+AHP WSP+ I+SA+MTT    DN+N SL D + G
Sbjct: 541 KTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTG 600

Query: 608 AFSNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITC-LK 667
             + P+ +G+GH++  +A++PGLVYDI+ +DYITFLCS+ YG   +Q I  R+ + C   
Sbjct: 601 KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVIT-RTPVRCPTT 660

Query: 668 KFADPGQLNYPSFSVVFGSKR--VVRYT--RIVTNVGAAGSVYEVATTAPSVVKVTVKPS 727
           +   PG LNYPS + VF + R  +V  T  R  TNVG A +VY     +P  V VTVKP 
Sbjct: 661 RKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPP 720

Query: 728 KLVFSKVGERKRYTVTFVASRDAARTTRYG--FGSIAW-SNDQHQVRSPV 760
           +LVF+   +R+ Y VT   +         G  FGS+ W    +H VRSP+
Sbjct: 721 RLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPI 756

BLAST of Carg12953 vs. ExPASy TrEMBL
Match: A0A6J1H8F1 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111461488 PE=3 SV=1)

HSP 1 Score: 1520.0 bits (3934), Expect = 0.0e+00
Identity = 766/766 (100.00%), Postives = 766/766 (100.00%), Query Frame = 0

Query: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
           MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660
           NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 767
           ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Carg12953 vs. ExPASy TrEMBL
Match: A0A6J1JIF3 (subtilisin-like protease SBT1.8 OS=Cucurbita maxima OX=3661 GN=LOC111484737 PE=3 SV=1)

HSP 1 Score: 1510.7 bits (3910), Expect = 0.0e+00
Identity = 761/766 (99.35%), Postives = 762/766 (99.48%), Query Frame = 0

Query: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
           MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVY LHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660
           NPWAHGAGHVDPHKALSPGL+YDISTNDYITFLCSLDYGIDHVQAI KRSNITC KKFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 767
           ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Carg12953 vs. ExPASy TrEMBL
Match: A0A5A7UKV3 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001560 PE=3 SV=1)

HSP 1 Score: 1425.2 bits (3688), Expect = 0.0e+00
Identity = 713/766 (93.08%), Postives = 739/766 (96.48%), Query Frame = 0

Query: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
           M SM R L+  FLLLLLLL CVF+NAK+TYIV MKHHALPS+YLTHHDWYSA LQSLSSS
Sbjct: 1   MDSMPRFLI-PFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSS 60

Query: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ++SDSLLYTYTS++HGFAA LD +E ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  SSSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CE+GPDFSPS
Sbjct: 121 FGLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+S RDQDGHGTHTASTAAGSHV NASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNK VALVYNKGSN+SSNMCLPGSL+PA VRGKVV+CDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRS + PTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
           GT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL+NDKRKTQ
Sbjct: 481 GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660
           NPWAHGAGHVDPHKALSPGL+YDISTNDYI FLCSLDYGIDHVQAI KRSNITC +KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVY+VATTAPSVVKVTVKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVG 720

Query: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 767
           ERKRYTVTFVASRDAA+TTR+GFGSI WSNDQHQVRSPV+FAWT+L
Sbjct: 721 ERKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765

BLAST of Carg12953 vs. ExPASy TrEMBL
Match: A0A1S3BQE8 (subtilisin-like protease SBT1.8 OS=Cucumis melo OX=3656 GN=LOC103492374 PE=3 SV=1)

HSP 1 Score: 1425.2 bits (3688), Expect = 0.0e+00
Identity = 713/766 (93.08%), Postives = 739/766 (96.48%), Query Frame = 0

Query: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
           M SM R L+  FLLLLLLL CVF+NAK+TYIV MKHHALPS+YLTHHDWYSA LQSLSSS
Sbjct: 1   MDSMPRFLI-PFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSS 60

Query: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ++SDSLLYTYTS++HGFAA LD +E ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  SSSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CE+GPDFSPS
Sbjct: 121 FGLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+S RDQDGHGTHTASTAAGSHV NASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNK VALVYNKGSN+SSNMCLPGSL+PA VRGKVV+CDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRS + PTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
           GT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL+NDKRKTQ
Sbjct: 481 GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660
           NPWAHGAGHVDPHKALSPGL+YDISTNDYI FLCSLDYGIDHVQAI KRSNITC +KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVY+VATTAPSVVKVTVKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVG 720

Query: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 767
           ERKRYTVTFVASRDAA+TTR+GFGSI WSNDQHQVRSPV+FAWT+L
Sbjct: 721 ERKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765

BLAST of Carg12953 vs. ExPASy TrEMBL
Match: A0A5D3CDV9 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001530 PE=3 SV=1)

HSP 1 Score: 1421.0 bits (3677), Expect = 0.0e+00
Identity = 711/764 (93.06%), Postives = 736/764 (96.34%), Query Frame = 0

Query: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60
           M SM R L+    LLLLLL CVF+NAK+TYIV MKHHALPS+YLTHHDWYSA LQSLSSS
Sbjct: 1   MDSMPRFLIP--FLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSS 60

Query: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ++SDSLLYTYTS++HGFAA LD +E ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  SSSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECE+GPDFSPS
Sbjct: 121 FGLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECEAGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+S RDQDGHGTHTASTAAGSHV NASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNK VALVYNKGSN+SSNMCLPGSL+PA VRGKVV+CDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRS + PTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540
           GT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL+NDKRKTQ
Sbjct: 481 GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660
           NPWAHGAGHVDPHKALSPGL+YDISTNDYI FLCSLDYGIDHVQAI KRSNITC +KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVY+VATTAPSVVKVTVKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVG 720

Query: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWT 765
           ERKRYTVTFVASRDAA+TTR+GFGSI WSNDQHQVRSPV+FAWT
Sbjct: 721 ERKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT 762

BLAST of Carg12953 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 548/759 (72.20%), Postives = 632/759 (83.27%), Query Frame = 0

Query: 8   LVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSATSDSLL 67
           ++ +FL LLL        AK+TYI+R+ H   P  +LTHHDWY++ L S S      SLL
Sbjct: 13  IITTFLFLLL-----HTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLL 72

Query: 68  YTYTSAYHGFAASLDPDEAE-LLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEG 127
           YTYT+++HGF+A LD  EA+ LL  S+S+L ++ED +Y LHTTRTP FLGL+S+FG+   
Sbjct: 73  YTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV--- 132

Query: 128 HNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKL 187
               DL  +S+ VIIGVLDTG+WPES+SFDDT MPEIPS+W+GECESG DF   LCNKKL
Sbjct: 133 ---HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKL 192

Query: 188 IGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIA 247
           IGARSFSKG+QMASGGG F   RE+ SPRD DGHGTHT++TAAGS V NAS LGYA G A
Sbjct: 193 IGARSFSKGFQMASGGG-FSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTA 252

Query: 248 RGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGA 307
           RGMA +ARVA YK CW TGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYYRDTIAIGA
Sbjct: 253 RGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGA 312

Query: 308 FAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSL 367
           F+AME+GVFVSCSAGNSGP +AS+ANVAPW+MTVGAGTLDRDFPA+  LGNGKR TGVSL
Sbjct: 313 FSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSL 372

Query: 368 YSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGGVVRDA 427
           YSG GMG KP+ LVYNKG++SSSN+CLPGSL+ + VRGK+V+CDRG+NARVEKG VVRDA
Sbjct: 373 YSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDA 432

Query: 428 GGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGGTVLNV 487
           GG+GMI+ANTAASGEELVADSHLLPA+AVG KTGDL+R+YV+S +KPTA+L F GTVL+V
Sbjct: 433 GGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDV 492

Query: 488 RPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSG 547
           +PSPVVAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGLD D R+TQFNIMSG
Sbjct: 493 KPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSG 552

Query: 548 TSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHG 607
           TSMSCPHISGLA LLKAAHP WSPSAIKSALMTTAY  DNTN+ L DAA  + SNP+AHG
Sbjct: 553 TSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHG 612

Query: 608 AGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFADPGQLNY 667
           +GHVDP KALSPGLVYDIST +YI FLCSLDY +DH+ AI KR ++ C KKF+DPGQLNY
Sbjct: 613 SGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNY 672

Query: 668 PSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGERKRYT 727
           PSFSV+FG KRVVRYTR VTNVGAA SVY+V       V ++VKPSKL F  VGE+KRYT
Sbjct: 673 PSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYT 732

Query: 728 VTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTK 766
           VTFV+ +  + T +  FGSI WSN QH+VRSPVAF+W +
Sbjct: 733 VTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNR 753

BLAST of Carg12953 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 802.7 bits (2072), Expect = 2.5e-232
Identity = 429/771 (55.64%), Postives = 546/771 (70.82%), Query Frame = 0

Query: 3   SMARLLVASFLLLLLLLPCVFVNA---KRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSS 62
           S + L   +F LLL L  C   ++   + TYIV M    +PS +  H +WY + L+S+S 
Sbjct: 2   SSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISD 61

Query: 63  SATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS 122
           SA    LLYTY +A HGF+  L  +EA+ L     V+ V  +  Y LHTTRTP FLGLD 
Sbjct: 62  SA---ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD- 121

Query: 123 DFGLWEGHNTQDL--DQASH-DVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPD 182
                   +T DL  +  S+ DV++GVLDTG+WPESKS+ D G   IPS W+G CE+G +
Sbjct: 122 -------EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTN 181

Query: 183 FSPSLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANA 242
           F+ SLCN+KLIGAR F++GY+  S  G   +S+E+ SPRD DGHGTHT+STAAGS V  A
Sbjct: 182 FTASLCNRKLIGARFFARGYE--STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGA 241

Query: 243 SLLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAP 302
           SLLGYA G ARGMAP+ARVA YK CW  GCF SDILA +D+AI+D V+VLS+SLGGG + 
Sbjct: 242 SLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSD 301

Query: 303 YYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLG 362
           YYRD +AIGAFAAME+G+ VSCSAGN+GP+ +SL+NVAPWI TVGAGTLDRDFPA   LG
Sbjct: 302 YYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILG 361

Query: 363 NGKRFTGVSLYSGQGMGNKPVALVY--NKGSNSSSNMCLPGSLEPAFVRGKVVICDRGIN 422
           NGK FTGVSL+ G+ + +K +  +Y  N  + ++ N+C+ G+L P  V+GK+V+CDRGIN
Sbjct: 362 NGKNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN 421

Query: 423 ARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPT 482
           ARV+KG VV+ AGG+GMILANTAA+GEELVAD+HLLPA  VG K GD+IR YV +   PT
Sbjct: 422 ARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT 481

Query: 483 AVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDN 542
           A +S  GTV+ V+PSPVVAAFSSRGPN +TP ILKPD+I PGVNILAAW+ + GPTGL +
Sbjct: 482 ASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLAS 541

Query: 543 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDA 602
           D R+ +FNI+SGTSMSCPH+SGLAALLK+ HP WSP+AI+SALMTTAY        L D 
Sbjct: 542 DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI 601

Query: 603 AGGAFSNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITC 662
           A G  S P+ HGAGHV P  A +PGL+YD++T DY+ FLC+L+Y    ++++++R N TC
Sbjct: 602 ATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRR-NYTC 661

Query: 663 -LKKFADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSK 722
              K      LNYPSF+V        +YTR VT+VG AG+     T+  + VK++V+P+ 
Sbjct: 662 DPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAV 721

Query: 723 LVFSKVGERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWT 765
           L F +  E+K YTVTF         +   FGSI WS+ +H V SPVA +WT
Sbjct: 722 LNFKEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Carg12953 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 767.7 bits (1981), Expect = 8.9e-222
Identity = 416/777 (53.54%), Postives = 523/777 (67.31%), Query Frame = 0

Query: 4   MARLLVASFLLLLLLLPCVF------VNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSL 63
           MA+L ++S   +  LL C F       +   +YIV ++    PS + +H++W+ + L+SL
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL 60

Query: 64  SSSATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 123
            SS    +LLY+Y+ A HGF+A L P +   LR+  SV+ V  D    +HTT TP FLG 
Sbjct: 61  PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 120

Query: 124 DSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDF 183
             + GLW   N         DVI+GVLDTGIWPE  SF D+G+  IPS W+GECE GPDF
Sbjct: 121 SQNSGLWSNSN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDF 180

Query: 184 SPSLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANAS 243
             S CN+KLIGAR+F +GY     G     ++E+ SPRD +GHGTHTASTAAGS VANAS
Sbjct: 181 PASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANAS 240

Query: 244 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLG-GGSAP 303
           L  YARG A GMA +AR+AAYK CW  GC+ SDILA MD+A++DGV V+SLS+G  GSAP
Sbjct: 241 LYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAP 300

Query: 304 -YYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQL 363
            Y+ D+IAIGAF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    
Sbjct: 301 EYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAIT 360

Query: 364 GNGKRFTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINA 423
           G+GK FTG SLY+G+ + +  ++LVY+   +  S +C PG L  + V GK+V+CDRG NA
Sbjct: 361 GDGKVFTGTSLYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNA 420

Query: 424 RVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTA 483
           RVEKG  V+ AGG GMILANTA SGEEL ADSHL+PA  VGAK GD IR Y+++   PTA
Sbjct: 421 RVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTA 480

Query: 484 VLSFGGTVLNVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDN 543
            +SF GT++    PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  +GPT LD 
Sbjct: 481 KISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDI 540

Query: 544 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDA 603
           D R+ QFNI+SGTSMSCPH+SGLAALL+ AHP WSP+AIKSAL+TTAY  +N+   + D 
Sbjct: 541 DPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDL 600

Query: 604 AGGAFSNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNI-- 663
           A G  SN + HGAGHVDP+KAL+PGLVYDI   +Y+ FLC++ Y    +    +   +  
Sbjct: 601 ATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYD 660

Query: 664 TC-LKKFADPGQLNYPSFSVVFGSK-RVVRYTRIVTNVGA-AGSVYEVATTAPSVVKVTV 723
            C   K    G LNYPSFSVVF S   VV+Y R+V NVG+   +VYEV   +P+ V++ V
Sbjct: 661 ACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDV 720

Query: 724 KPSKLVFSKVGERKRYTVTF---VASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAW 764
            PSKL FSK      Y VTF   V          + FGSI W++ +H V+SPVA  W
Sbjct: 721 SPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Carg12953 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 749.6 bits (1934), Expect = 2.5e-216
Identity = 396/775 (51.10%), Postives = 515/775 (66.45%), Query Frame = 0

Query: 12  FLLLLLLLPCVFVNA--------KRTYIVRMKHHALPSDYLTHHDWYSAHLQSLS----- 71
           FL ++L +  +F+ A        K+TY++ M   A+P  Y  H  WYS+ + S++     
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 72  -SSATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 131
                ++ +LYTY +A+HG AA L  +EAE L + D V+ V  +T Y LHTTR+P FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 132 DSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDF 191
           +      E           HDV++GVLDTGIWPES+SF+DTGM  +P+ WRG CE+G  F
Sbjct: 131 ERQ----ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRF 190

Query: 192 SPSLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANAS 251
               CN+K++GAR F +GY+ A+  G   +  E +SPRD+DGHGTHTA+T AGS V  A+
Sbjct: 191 LKRNCNRKIVGARVFYRGYEAAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGAN 250

Query: 252 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 311
           L G+A G ARGMA +ARVAAYK CW  GCF SDIL+ +D+A++DGV VLS+SLGGG + Y
Sbjct: 251 LFGFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTY 310

Query: 312 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 371
            RD+++I  F AME GVFVSCSAGN GP+  SL NV+PWI TVGA T+DRDFPA V++G 
Sbjct: 311 SRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGT 370

Query: 372 GKRFTGVSLYSGQGM--GNKPVALVY---NKGSNSSSNMCLPGSLEPAFVRGKVVICDRG 431
            + F GVSLY G+ +   NK   LVY   N  S   ++ CL G+L+   V GK+VICDRG
Sbjct: 371 MRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRG 430

Query: 432 INARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAK 491
           +  RV+KG VV+ AGGIGM+L NTA +GEELVADSH+LPAVAVG K G LI+QY  +  K
Sbjct: 431 VTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKK 490

Query: 492 PTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL 551
            TA L   GT + ++PSPVVAAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L
Sbjct: 491 ATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSL 550

Query: 552 DNDKRKTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLR 611
            +D R+ +FNI+SGTSMSCPH+SG+AAL+K+ HP WSP+AIKSALMTTAY  DN    L 
Sbjct: 551 SSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLT 610

Query: 612 DAAGGAFSNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNI 671
           DA+G A S+P+ HGAGH+DP +A  PGLVYDI   +Y  FLC+ D     ++   K SN 
Sbjct: 611 DASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNR 670

Query: 672 TCLKKFA-DPGQLNYPSFSVVFGSK---RVVRYTRIVTNVGAAGSVYEVATTAPSVVKVT 731
           TC    A +PG LNYP+ S +F      + +   R VTNVG   S Y+V+ +      VT
Sbjct: 671 TCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVT 730

Query: 732 VKPSKLVFSKVGERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAW 764
           V+P  L F+   ++  YTVTF   R   R  R  FG + W +  H+VRSPV   W
Sbjct: 731 VQPKTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Carg12953 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 729.2 bits (1881), Expect = 3.5e-210
Identity = 392/770 (50.91%), Postives = 523/770 (67.92%), Query Frame = 0

Query: 8   LVASFLLLLLLLPCVFVN-----AKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSAT 67
           + +S ++LLL L   F++     A +T+I R+   ++PS + TH+ WYS         A 
Sbjct: 1   MASSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEF------AE 60

Query: 68  SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFG 127
              +++ Y + +HGF+A + PDEA+ LR   +VL V+ED    LHTTR+P FLGL +  G
Sbjct: 61  ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKG 120

Query: 128 LWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLC 187
           LW      + D  S DVIIGV DTGIWPE +SF D  +  IP RWRG CESG  FSP  C
Sbjct: 121 LW-----SESDYGS-DVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNC 180

Query: 188 NKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLGYA 247
           N+K+IGAR F+KG Q A  GG   K+ E  SPRD DGHGTHT+STAAG H   AS+ GYA
Sbjct: 181 NRKIIGARFFAKGQQAAVIGG-INKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYA 240

Query: 248 RGIARGMAPQARVAAYKTCW-PTGCFGSDILAGMDRAISDGVDVLSLSLGGG---SAPYY 307
            G+A+G+AP+AR+AAYK CW  +GC  SDILA  D A+ DGVDV+S+S+GGG   ++PYY
Sbjct: 241 SGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYY 300

Query: 308 RDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNG 367
            D IAIG++ A  KG+FVS SAGN GPN  S+ N+APW+ TVGA T+DR+FPA   LG+G
Sbjct: 301 LDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDG 360

Query: 368 KRFTGVSLYSGQGMGNKPVALVY-NKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARV 427
            R  GVSLY+G  +  +   +VY  K   SS+++C+  +L+P  VRGK+VICDRG + RV
Sbjct: 361 HRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRV 420

Query: 428 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVL 487
            KG VV+ AGG+GMILAN A++GE LV D+HL+PA AVG+  GD I+ Y  S   P A +
Sbjct: 421 AKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASI 480

Query: 488 SFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 547
            F GT++ ++P+PV+A+FS RGPN ++P+ILKPD+I PGVNILAAW++++GPTGL +D R
Sbjct: 481 DFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPR 540

Query: 548 KTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 607
           KT+FNI+SGTSM+CPH+SG AALLK+AHP WSP+ I+SA+MTT    DN+N SL D + G
Sbjct: 541 KTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTG 600

Query: 608 AFSNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITC-LK 667
             + P+ +G+GH++  +A++PGLVYDI+ +DYITFLCS+ YG   +Q I  R+ + C   
Sbjct: 601 KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVIT-RTPVRCPTT 660

Query: 668 KFADPGQLNYPSFSVVFGSKR--VVRYT--RIVTNVGAAGSVYEVATTAPSVVKVTVKPS 727
           +   PG LNYPS + VF + R  +V  T  R  TNVG A +VY     +P  V VTVKP 
Sbjct: 661 RKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPP 720

Query: 728 KLVFSKVGERKRYTVTFVASRDAARTTRYG--FGSIAW-SNDQHQVRSPV 760
           +LVF+   +R+ Y VT   +         G  FGS+ W    +H VRSP+
Sbjct: 721 RLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPI 756