Carg12919 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12919
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	2-oxoisovalerate dehydrogenase subunit beta
Location	Carg_Chr10: 3784224 .. 3790108 (-)
RNA-Seq Expression	Carg12919
Synteny	Carg12919

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTTTTTGTTGAGTGTTCGATTCGGTATTATCTGTTTGAAGCTTTTCCAGTTCTTTGTTAGGCATAAAAACGTGTGAAATTAAGCCACAATTCGTTCATCTCTTCCGAAATGGTATCGATTTTGAGGAGATCGCCGAAACTGTTGAACTCAATTTGTAGGAGGCGATTTTCTGCTTCCTCTGATGGAAATCTTACGAGCCATGGAGGAAAATCTATGAATCTGTATTCTGCTATCAATCAAGCGCTTCATATTGCCCTTGAAACTGATCCTCGGTGTGTTTCTTTATTACGCTTTTTTTGTTTTTTGGTTTGTGATCGCCTGTAGTTTTGATAAAATCTTCTGTGATTCTGTGTTGCTTTTTTCGCAAATGGTTGTGGTTTTTCTTTGATGAGTTTGCTGTGTTTTTCGACTTTCTGCTGTTTGGTTGCTGTTTTGCTTGAGATTTTTGAGTTGTTAGCTAGTGAGAGCTTTCATGGAAATGAAAGTGTGAGTGTAACAGTTCTTTGTTAGACATAAAAACGTGTGAAATTAAGTCACAATTCGCTCATCTCTTCCGAAATGGTCATCCGTTCAATCTGGAATCTATTATTCCCGTCGTTGACTACTTCTTCCTTTGTATCGATTTTGAGGAGATCGCCGAAACTGTCGAACTCCATTTGTAGGAGGCGATTTTCTGCTTCCTCTGATGGAAATCTTACGAGCCATGGAGGAAAATCTATGAATCTGTATTCTGCTATCAATCAAGCGCTTCATATTGCCCTTGAAACTGATCCTCGGTGTGTTTCTTTATTACGCTTCTTTTTGTTTTTTGGTTTGTGATCGTCTGTAGTTTTGATAAAATCTTCTGTGATTCTGTGTTGCTTTTTTCGCAAATGGTTGTGGTTTTTCTTTGATGAGTTTGCTGTGTTTTTCGACTTTCTGCTGTTTGGTTGCTGTTTTGCTTGAGATTTTTGAGTTGTTAGCTAGTGAGAGCTTGCATGGAAATGAAAGTGAAGGAAATTTGATGAAATCTTAAGAGCTTTATTTGGATTTGATCGGACGATTCTTCCGCAGATTGATGAATCTTGTGGAACTGTTAATTTCTGAGCGTGTTCTTTCTGTTCTTCATGTGATTAAATCTTGGGGCAACAGTGACCTATCAGTTTCTTTGGGCTAATTGGACTTGTTAGAGTCATCCTTTCAAGCACAAAGCTAAAGAGTTCTAGGAATAAGCTACCGAAGGCTTTGTTTTGGTAAGTTTTTGGTAGGAAGAAACCAAAGATATTTCAGAGGAGATCGAGAGATTTCAGATATTGTTCTTCGTTCTAGGGCGTCCTGTTCGAGTTCTTCTATATAAACATATATCTTTTTGATGAATGGGAATTGAGCTGTTTTCTTAACACCCTTTGTTAACTGTTTAATGTTCTGGTAGCAATTTTGTTGTTTAAGAACAATATACTTCCACATTCAGTTCTTATGTGTTTGGAGAAGACGTGGGCTTCGGTGGGGTCTTCCGTTGCACAACTGGATTAGTGGATCGATATGGAAAAGATCGAGTTTTCAATACCCCTCTTTGTGAGCAGGTAGCCTACTTGTTGACTTTGAAATTTTGGAGGTCGATATGATAGTACAGCCATTGTGAAAAGCTTATATCATGTGATCAATATTCTATTATCGAGTATATGTGAAAAGCTTCATTTTAACTCATTTTTAATACAGGGAATTGTTGGCTTTGGCATTGGTCTTGCAGCAATGGTAATTTTGAGGAATTTGCATACTTAGCCATGTGATGGAATTTCTTTGTTTCTCACATCTTTTCCCTTTTCTAACGCGGAAGTCGTTTCACGTGGTAGGGAAATAGGGCCATTGCAGAAATTCAGTTTGCAGATTACATTTATCCAGCTTTTGATCAGGTAAGCTTTCGGCTTCGTTGAGATTCACCCTCCTGCCATCTTGTTTGGCATTATCTTGAGAAAATTTTTGGCCTTCGTAGATAGTTAATGAAGCTGCAAAGTTCAGATATCGTAGCGGAAATCAGTTCAATTGTGGTGGTAGATTTCCCCCCCTCTTTGCATACAGCCAACTTCTTTCATGTTTACTTTCTTGTTATCGGCCCTTCATGTCACGCATATTTACGAATCCTCTCAGGTTTAACAATTAGAGCCCCATATGGAGCTGTTGGTCATGGTGGTCATTATCACTCACAATCACCTGAAGCTTTCTTCTGTCATGTTCCTGGTATCAAAGTATGTTCCCGTCTTCTGCTAATTTGTCTTGATAAGTTTTAGGTATTTTCAGTACTTCGTGACAAATATGTAACAATCCAAGCTCACCGCTAGCAGATATTGTTCTCTTTGGCCCGTTACGTATCACCGTCAGCCTCATGGTTTCAAAACACGTCTACTAGGAAGAGGTTTCCACACCCTTATAAGAGATGTTTCGTTCCCCTCTCCAACCGATGTGAGATCTCATGATCCACCCCCTTGGAGGCCCAGTGTCCTCGCTGGACACTACCCGGTGTCTGGCTATGATACCATTTGTAACAGTTCAAGCTCATAGCTTGCCGATATCATCTTCTTTGGCCCGTTATATATCGGCGTTAGCGTTATTTATAGATTAAGGAGCCTTCATTTATGACCCATATGTTGGCATCCAATGTATTGGCAGAGGGTCTTCTGACGAGCAAAGGATAGAGTTTTTATGATCCAAAAGTTAGCATCCCTATCTGACCGTAAAACCAGCAGTCATCTTGAAGAAGTTCAGAACTGGAGGAAAGGGGCCAGAATTTCACCTCTCTTACCTCTAGTGTGTAGACCATGCCATCTTTTCTTGCTGCCGTGTAGTCTCAATCATTTCTTGTAGAATAAACTTAACTTAGGACGTGTCTCAGTTATTTTGCTATTATATTAACTTCATTGCATGAATTATTACTTTTTTGGAAAACCATAATTTTATCGTTTTCATGCTAAACAGGTTGTCATCCCTCGAAGTCCATACCAAGCCAAGGGATTGCTACTTTCATGCATCCGTGATCAAAACCCTGTTGTATTTTTTGAGCCCAAGGTATGAAAACTTTTCTAAGTTGTTTGGATCGATGTTTGTCCAGAAGTTGGGATGAAAATATTTTGAGCGTTCGTCTTCAGTGGCTTTACCGTTTGGCTGTTGAAGAAGTTCCTGAGCATGACTTCATGTTGCCTCTATCAGAAGCAGAGGTTTGATCTCATCTCTTTTCATGTTCTAATTATTTATGAATAATTCCATAGTGCATTAATAACGCAATGTTTGATAAACCTGAAAACGAGATATTCAGGTGATCCGAGAAGGCAGTGATATTACATTGGTTGGTTGGGGAGCGCAGCTTTCTGTTATGGAACAGGCCTGTATTGATGCTGAAAAAGTAAATCTCTTTACTTATTTTCTGGTATCTGTATTGTATGACAATGGAATTTTATATGCATCTGAATGGCAGGCTGAATTTCTTTCACTATTTTACTTACTGGTGTAAAGGGAAACCTTTCCATCTCGCTCTCCTTGGCTTTCAACTTATGTTCTCCTATCTGTTCATGTGTCGTTATTTTCTGCTTTTATTTAGTAATCGGGTTGGGTCTCTATCCCTTGCATTATGTACCCTTGGCCCACTCTACTAATTTCTCGGGAGGCTTCATGTAACAGCCACAGCGCACCGCTAGCAGATATTGTCCTCTTTGGGCTTTTCCTTTTAGGCTTCCCTTCAAGGTTTTTATAACGTGTCTCCTAGGGAGAGGTTTCCATACCCCTACAAATCGTTCACCTCCCAACCGATGTAGGATCTCACAATTCACTCTCCTTCGGGACCCAGCGTCCTCGCTGGCACTCGTTCAGCTCCCTAATCGAAGTGGCCAAGTTCATAAATAACTAAAATGATATTACAATGGTGAAGCTTAATTACTGTAGGATGAAAGCTTTTCAACATTCAATCTGAAACTTTGAAGAATATATGTAAACTGTTTAATTTTTGACATTTCAGTCTGTGAAGTCGCTAGGATCCAAGAAAATAATCAACTAATGATCTCGATTTGCTCGATATATATTGCAGGATGGAATCTCCTGTGAACTGATTGACCTGAGAACTCTAATACCATGGGATAAGGAAACGGTGGAGGCCTCAGTTAAGAAGACTGGGAGACTTCTTGTAAGTAATATTTTCGCTTCTTTATTACAAGCTTCCTGAGAAATGAGAACTTTAAGTGAAACAGATATAGATTTATAACTAATCTCCATTTCGCTCTGTAGATTAGCCATGAAGCTCCGGTTACCGGAGGCTTTGGTGCTGAAATATCCGCTTCCATTGTTGAACGCTGCTTCTTACGGGTGAGTGCGGATTTTCAGCATATTTCTATTGGTAAAAGTACATTTTAAAAAAGATTAGGAATCACAAACAGAGAGATGAATTTACTCCCTCGCTGATTAGACAGGGGCAGGTAAACTCTCCCTTCCCCTCCAACTTTCCTGAACAGACAAAGCATGGAATATACTCTCTCCTTCCCCTGTGTAAATACTCCAACATTCCCTTGGTGGCCCTCCTGTTGCTTATTCCCCCTCGGCAAGTGTAACAGTCCAAGCCCATTGCTAGCAGATATTATCCTCTTTGGACTTTCCTTTTTGGGCTCCTCCTTAACGTTTTTAAAATGCATTTACTAGAGAGAGGTTTCCACACCCTTATAAAGAATGCTTCGCTCCCCTCTCCAACCAACGTGAGATCTGACAATTCACCCCCCTTCAAGGATCAACGTCCTCGCTAGCATTCGTCCCTCTCTCCAATCGATGTGTGATCTCATTATCCACCCCGTCAAGGCCTAGTGTCCTCGTTGGTGCACCGTCCGGTGTCCACCCCCTTTGGGGCTAGCGTCCTCACTGGCACACTACTTGATGTCTGCGCTGATACCTCTGATACCATTTGTAACAGCTCAAGCCCACCACTAGCATATATTGTCTTCTTTGGGTTTTTCGTTTCAGACTTCTCCTCAAAGATTTAAAACTCGTATGCTAGGGAGAGGTTTTCACACCTTATAAAGAAGTTTTGTTCCTCTCTTCAACCGATGTGAAATCTCACAATCCAATCCCCTTTGAGACCCAACGTCCTTGCTGGCACTCGTTCCTCTCTCTAATCGATGTGAAATCTCACAGTAACTAACTTGTCGAGTGTGAATTTTCCTTCGTACTCGTCCTAGCGTACTGGATAGTAATGGGTGGCCTTACATCACATTTCTCTTTTTAGTAGGCTAAGACCTTTTATCTCACACCCCCAATCCAAGGAACCTTAAACTCTAGTTTGAAATTTGGAAGGTGAAGGCTTGTCCAAAGAACATGAACAATGGCTTGTAGGAAGCAACTAGTTTTATGGATATACTTCGATGTTCTTACTTTTGTTTGAGTACTCAATACAAGTAGAGTAGATAAAATGAGTATCTCTTTCTCTTGTAGTTGGAGGCCCCTGTTTCCAGAGTTTGTGGATTGGACACTCCATTCCCTCTTGTGTTTGAACCATTCTACATGCCTACTAAGAACAAAGTAAGAGACTACCTTCCATCTTCTTGCAAAAGATTTGGCATAATGTTCATTCCTAACCAGTATATTACCTCTCTCTCTGGCTGGTGTAGATATTGGACGCCATCAAAGCAACTGTAAAGTATTAGATCAAAAAATGGCTGCTTTCAAGCAGACAACAAGAAATGTTCTTCATTCCATCAGTCTCTATCGATGAACTAGATGATGTAGCCTTGATGATGTCCCATTGTTGGTGGCTGTAAGGGCCTTCTCTTGTTGAATAAGATAGTTTGAGTCAATGATGGCTGGATATCAAGATCCTTGGCAATAAAAAGTTACTATATTTATGAGAGTATATATTTACCTTTAGAAATAAAT

mRNA sequence

AATTTTTTGTTGAGTGTTCGATTCGGTATTATCTGTTTGAAGCTTTTCCAGTTCTTTGTTAGGCATAAAAACGTGTGAAATTAAGCCACAATTCGTTCATCTCTTCCGAAATGGTATCGATTTTGAGGAGATCGCCGAAACTGTTGAACTCAATTTGTAGGAGGCGATTTTCTGCTTCCTCTGATGGAAATCTTACGAGCCATGGAGGAAAATCTATGAATCTGTATTCTGCTATCAATCAAGCGCTTCATATTGCCCTTGAAACTGATCCTCGGAGATCGCCGAAACTGTCGAACTCCATTTGTAGGAGGCGATTTTCTGCTTCCTCTGATGGAAATCTTACGAGCCATGGAGGAAAATCTATGAATCTGTATTCTGCTATCAATCAAGCGCTTCATATTGCCCTTGAAACTGATCCTCGTTCTTATGTGTTTGGAGAAGACGTGGGCTTCGGTGGGGTCTTCCGTTGCACAACTGGATTAGTGGATCGATATGGAAAAGATCGAGTTTTCAATACCCCTCTTTGTGAGCAGGGAATTGTTGGCTTTGGCATTGGTCTTGCAGCAATGGGAAATAGGGCCATTGCAGAAATTCAGTTTGCAGATTACATTTATCCAGCTTTTGATCAGATAGTTAATGAAGCTGCAAAGTTCAGATATCGTAGCGGAAATCAGTTCAATTGTGGTGGTTTAACAATTAGAGCCCCATATGGAGCTGTTGGTCATGGTGGTCATTATCACTCACAATCACCTGAAGCTTTCTTCTGTCATGTTGTCATCCCTCGAAGTCCATACCAAGCCAAGGGATTGCTACTTTCATGCATCCGTGATCAAAACCCTGTTGTATTTTTTGAGCCCAAGTGGCTTTACCGTTTGGCTGTTGAAGAAGTTCCTGAGCATGACTTCATGTTGCCTCTATCAGAAGCAGAGGTGATCCGAGAAGGCAGTGATATTACATTGGTTGGTTGGGGAGCGCAGCTTTCTGTTATGGAACAGGCCTGTATTGATGCTGAAAAAGATGGAATCTCCTGTGAACTGATTGACCTGAGAACTCTAATACCATGGGATAAGGAAACGGTGGAGGCCTCAGTTAAGAAGACTGGGAGACTTCTTATTAGCCATGAAGCTCCGGTTACCGGAGGCTTTGGTGCTGAAATATCCGCTTCCATTGTTGAACGCTGCTTCTTACGGTTGGAGGCCCCTGTTTCCAGAGTTTGTGGATTGGACACTCCATTCCCTCTTGTGTTTGAACCATTCTACATGCCTACTAAGAACAAAATATTGGACGCCATCAAAGCAACTGTAAAGTATTAGATCAAAAAATGGCTGCTTTCAAGCAGACAACAAGAAATGTTCTTCATTCCATCAGTCTCTATCGATGAACTAGATGATGTAGCCTTGATGATGTCCCATTGTTGGTGGCTGTAAGGGCCTTCTCTTGTTGAATAAGATAGTTTGAGTCAATGATGGCTGGATATCAAGATCCTTGGCAATAAAAAGTTACTATATTTATGAGAGTATATATTTACCTTTAGAAATAAAT

Coding sequence (CDS)

ATGGTATCGATTTTGAGGAGATCGCCGAAACTGTTGAACTCAATTTGTAGGAGGCGATTTTCTGCTTCCTCTGATGGAAATCTTACGAGCCATGGAGGAAAATCTATGAATCTGTATTCTGCTATCAATCAAGCGCTTCATATTGCCCTTGAAACTGATCCTCGGAGATCGCCGAAACTGTCGAACTCCATTTGTAGGAGGCGATTTTCTGCTTCCTCTGATGGAAATCTTACGAGCCATGGAGGAAAATCTATGAATCTGTATTCTGCTATCAATCAAGCGCTTCATATTGCCCTTGAAACTGATCCTCGTTCTTATGTGTTTGGAGAAGACGTGGGCTTCGGTGGGGTCTTCCGTTGCACAACTGGATTAGTGGATCGATATGGAAAAGATCGAGTTTTCAATACCCCTCTTTGTGAGCAGGGAATTGTTGGCTTTGGCATTGGTCTTGCAGCAATGGGAAATAGGGCCATTGCAGAAATTCAGTTTGCAGATTACATTTATCCAGCTTTTGATCAGATAGTTAATGAAGCTGCAAAGTTCAGATATCGTAGCGGAAATCAGTTCAATTGTGGTGGTTTAACAATTAGAGCCCCATATGGAGCTGTTGGTCATGGTGGTCATTATCACTCACAATCACCTGAAGCTTTCTTCTGTCATGTTGTCATCCCTCGAAGTCCATACCAAGCCAAGGGATTGCTACTTTCATGCATCCGTGATCAAAACCCTGTTGTATTTTTTGAGCCCAAGTGGCTTTACCGTTTGGCTGTTGAAGAAGTTCCTGAGCATGACTTCATGTTGCCTCTATCAGAAGCAGAGGTGATCCGAGAAGGCAGTGATATTACATTGGTTGGTTGGGGAGCGCAGCTTTCTGTTATGGAACAGGCCTGTATTGATGCTGAAAAAGATGGAATCTCCTGTGAACTGATTGACCTGAGAACTCTAATACCATGGGATAAGGAAACGGTGGAGGCCTCAGTTAAGAAGACTGGGAGACTTCTTATTAGCCATGAAGCTCCGGTTACCGGAGGCTTTGGTGCTGAAATATCCGCTTCCATTGTTGAACGCTGCTTCTTACGGTTGGAGGCCCCTGTTTCCAGAGTTTGTGGATTGGACACTCCATTCCCTCTTGTGTTTGAACCATTCTACATGCCTACTAAGAACAAAATATTGGACGCCATCAAAGCAACTGTAAAGTATTAG

Protein sequence

MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRRSPKLSNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY

Homology

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BLAST of Carg12919 vs. NCBI nr
Match: KAG7023738.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 815.5 bits (2105), Expect = 2.1e-232
Identity = 400/400 (100.00%), Postives = 400/400 (100.00%), Query Frame = 0


Query: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRRSPKL 60
           MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRRSPKL
Sbjct: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRRSPKL 60

Query: 61  SNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRC 120
           SNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRC
Sbjct: 61  SNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRC 120

Query: 121 TTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAK 180
           TTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAK
Sbjct: 121 TTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAK 180

Query: 181 FRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVVIPRSPYQAKGLLLSCIRD 240
           FRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVVIPRSPYQAKGLLLSCIRD
Sbjct: 181 FRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVVIPRSPYQAKGLLLSCIRD 240

Query: 241 QNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLSVMEQACIDA 300
           QNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLSVMEQACIDA
Sbjct: 241 QNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLSVMEQACIDA 300

Query: 301 EKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLR 360
           EKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLR
Sbjct: 301 EKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLR 360

Query: 361 LEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           LEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 361 LEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 400

[Close]

BLAST of Carg12919 vs. NCBI nr
Match: XP_022960762.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita moschata] >KAG6590071.1 2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 703.7 bits (1815), Expect = 8.8e-199
Identity = 345/351 (98.29%), Postives = 345/351 (98.29%), Query Frame = 0


Query: 55  RRSPKLSNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 114
           RRSPKL NSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF
Sbjct: 6   RRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 65

Query: 115 GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 174
           GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI
Sbjct: 66  GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 125

Query: 175 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPYQ 234
           VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH     VVIPRSPYQ
Sbjct: 126 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQ 185

Query: 235 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ 294
           AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ
Sbjct: 186 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ 245

Query: 295 LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 354
           LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI
Sbjct: 246 LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 305

Query: 355 SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 306 SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

[Close]

BLAST of Carg12919 vs. NCBI nr
Match: XP_023515905.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 702.2 bits (1811), Expect = 2.6e-198
Identity = 344/351 (98.01%), Postives = 344/351 (98.01%), Query Frame = 0


Query: 55  RRSPKLSNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 114
           RRSPKL NSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF
Sbjct: 6   RRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 65

Query: 115 GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 174
           GGVFRCTTGL DRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI
Sbjct: 66  GGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 125

Query: 175 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPYQ 234
           VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH     VVIPRSPYQ
Sbjct: 126 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQ 185

Query: 235 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ 294
           AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ
Sbjct: 186 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ 245

Query: 295 LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 354
           LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI
Sbjct: 246 LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 305

Query: 355 SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 306 SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

[Close]

BLAST of Carg12919 vs. NCBI nr
Match: XP_022987320.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 690.6 bits (1781), Expect = 7.7e-195
Identity = 340/351 (96.87%), Postives = 343/351 (97.72%), Query Frame = 0


Query: 55  RRSPKLSNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 114
           RRSPKL +SICRR+FSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF
Sbjct: 6   RRSPKLLSSICRRQFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 65

Query: 115 GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 174
           GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI
Sbjct: 66  GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 125

Query: 175 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPYQ 234
           VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH     VVIPRSPYQ
Sbjct: 126 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQ 185

Query: 235 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ 294
           AKGLLLSCIRDQNPVVFFEPK LYRLAVEEVPE DFMLPLSEAEVIREGSDITLVGWGAQ
Sbjct: 186 AKGLLLSCIRDQNPVVFFEPKLLYRLAVEEVPEDDFMLPLSEAEVIREGSDITLVGWGAQ 245

Query: 295 LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 354
           LSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVTGGFGAEI
Sbjct: 246 LSVMEQACIDAEKDGISCELIDLRTLMPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 305

Query: 355 SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 306 SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

[Close]

BLAST of Carg12919 vs. NCBI nr
Match: XP_008450996.1 (PREDICTED: 2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucumis melo])

HSP 1 Score: 676.4 bits (1744), Expect = 1.5e-190
Identity = 326/351 (92.88%), Postives = 339/351 (96.58%), Query Frame = 0


Query: 55  RRSPKLSNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 114
           RRS  + NS+CRR+FSAS DGNLT+H GKSMNLYSAINQALHIALETDPR+YVFGEDVGF
Sbjct: 6   RRSQTVLNSLCRRQFSASPDGNLTTHAGKSMNLYSAINQALHIALETDPRAYVFGEDVGF 65

Query: 115 GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 174
           GGVFRCTTGL DRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI
Sbjct: 66  GGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 125

Query: 175 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPYQ 234
           VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH     VVIPRSPYQ
Sbjct: 126 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQ 185

Query: 235 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ 294
           AKGLLLSCIRD+NPV+FFEPKWLYRLAVEEVPE DFMLPLS+AEVIREGSDITLVGWGAQ
Sbjct: 186 AKGLLLSCIRDENPVIFFEPKWLYRLAVEEVPEDDFMLPLSQAEVIREGSDITLVGWGAQ 245

Query: 295 LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 354
           LSVMEQAC+DAEK+GISCELIDLRTL+PWDKETVEASV+KTGRLLISHEAPVTGGFGAEI
Sbjct: 246 LSVMEQACVDAEKEGISCELIDLRTLLPWDKETVEASVRKTGRLLISHEAPVTGGFGAEI 305

Query: 355 SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           SASIVERCFLRLEAP+SRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 306 SASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

[Close]

BLAST of Carg12919 vs. ExPASy Swiss-Prot
Match: Q9SAV3 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BCDH BETA1 PE=2 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 8.3e-176
Identity = 299/348 (85.92%), Postives = 322/348 (92.53%), Query Frame = 0


Query: 60  LSNSICRRRFSASSDG--NLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGV 119
           L  S  +  F + S G   +++  GK +NLYSAINQALHIAL+TDPRSYVFGEDVGFGGV
Sbjct: 5   LGRSCRKLSFPSLSHGARRVSTETGKPLNLYSAINQALHIALDTDPRSYVFGEDVGFGGV 64

Query: 120 FRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNE 179
           FRCTTGL +R+GK+RVFNTPLCEQGIVGFGIGLAAMGNRAI EIQFADYIYPAFDQIVNE
Sbjct: 65  FRCTTGLAERFGKNRVFNTPLCEQGIVGFGIGLAAMGNRAIVEIQFADYIYPAFDQIVNE 124

Query: 180 AAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPYQAKG 239
           AAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH     VVIPRSP +AKG
Sbjct: 125 AAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAKG 184

Query: 240 LLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLSV 299
           LLLSCIRD NPVVFFEPKWLYR AVEEVPEHD+M+PLSEAEVIREG+DITLVGWGAQL+V
Sbjct: 185 LLLSCIRDPNPVVFFEPKWLYRQAVEEVPEHDYMIPLSEAEVIREGNDITLVGWGAQLTV 244

Query: 300 MEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISAS 359
           MEQAC+DAEK+GISCELIDL+TL+PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA+
Sbjct: 245 MEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISAT 304

Query: 360 IVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           I+ERCFL+LEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIK+TV Y
Sbjct: 305 ILERCFLKLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY 352

[Close]

BLAST of Carg12919 vs. ExPASy Swiss-Prot
Match: Q9LDY2 (2-oxoisovalerate dehydrogenase subunit beta 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DIN4 PE=1 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 3.3e-172
Identity = 295/352 (83.81%), Postives = 315/352 (89.49%), Query Frame = 0


Query: 67  RRFSASSDGNLTSHG-------------GKSMNLYSAINQALHIALETDPRSYVFGEDVG 126
           RRF   S   ++ HG             GKSMNLYSAINQALHIALETDPRSYVFGEDVG
Sbjct: 7   RRFCRGSSFPVSGHGYRMLSTVENVSESGKSMNLYSAINQALHIALETDPRSYVFGEDVG 66

Query: 127 FGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQ 186
           FGGVFRCTTGL +R+GK RVFNTPLCEQGIVGFGIGLAAMGNR IAEIQFADYI+PAFDQ
Sbjct: 67  FGGVFRCTTGLAERFGKSRVFNTPLCEQGIVGFGIGLAAMGNRVIAEIQFADYIFPAFDQ 126

Query: 187 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPY 246
           IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH     VVIPRSP 
Sbjct: 127 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPR 186

Query: 247 QAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGA 306
           +AKGLLLS IRD NPVVFFEPKWLYR AVE+VPE D+M+PLSEAEV+REGSDITLVGWGA
Sbjct: 187 EAKGLLLSSIRDPNPVVFFEPKWLYRQAVEDVPEDDYMIPLSEAEVMREGSDITLVGWGA 246

Query: 307 QLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAE 366
           QL++MEQAC+DAE +GISCELIDL+TLIPWDKE VE SV+KTGRLLISHEAPVTGGFGAE
Sbjct: 247 QLTIMEQACLDAENEGISCELIDLKTLIPWDKEIVETSVRKTGRLLISHEAPVTGGFGAE 306

Query: 367 ISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           I+A+IVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAI++TV Y
Sbjct: 307 IAATIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIRSTVNY 358

[Close]

BLAST of Carg12919 vs. ExPASy Swiss-Prot
Match: Q6P3A8 (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Mus musculus OX=10090 GN=Bckdhb PE=1 SV=2)

HSP 1 Score: 465.7 bits (1197), Expect = 5.3e-130
Identity = 222/324 (68.52%), Postives = 262/324 (80.86%), Query Frame = 0


Query: 83  KSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLVDRYGKDRVFNTPLCEQG 142
           + MNL+ +I  AL  +L  DP + +FGEDV FGGVFRCT GL D+YGKDRVFNTPLCEQG
Sbjct: 67  QKMNLFQSITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQG 126

Query: 143 IVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGA 202
           IVGFGIG+A  G  AIAEIQFADYI+PAFDQIVNEAAK+RYRSG+ FNCG LTIRAP+G 
Sbjct: 127 IVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGC 186

Query: 203 VGHGGHYHSQSPEAFFCH-----VVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAV 262
           VGHG  YHSQSPEAFF H     VVIPRSP+QAKGLLLSCI D+NP +FFEPK LYR AV
Sbjct: 187 VGHGALYHSQSPEAFFAHCPGIKVVIPRSPFQAKGLLLSCIEDKNPCIFFEPKILYRAAV 246

Query: 263 EEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLSVM-EQACIDAEKDGISCELIDLRTLI 322
           E+VP   + +PLS+AEVI+EGSD+TLV WG Q+ V+ E A +  EK G+SCE+IDLRT++
Sbjct: 247 EQVPVEPYKIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRTIV 306

Query: 323 PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPVSRVCGLDTPFP 382
           PWD +TV  SV KTGRLLISHEAP+TGGF +EIS+++ E CFL LEAP+SRVCG DTPFP
Sbjct: 307 PWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFP 366

Query: 383 LVFEPFYMPTKNKILDAIKATVKY 401
            +FEPFY+P K K  DA++  + Y
Sbjct: 367 HIFEPFYIPDKWKCYDALRKMINY 390

[Close]

BLAST of Carg12919 vs. ExPASy Swiss-Prot
Match: P21839 (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Bos taurus OX=9913 GN=BCKDHB PE=1 SV=2)

HSP 1 Score: 464.2 bits (1193), Expect = 1.5e-129
Identity = 219/324 (67.59%), Postives = 262/324 (80.86%), Query Frame = 0


Query: 83  KSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLVDRYGKDRVFNTPLCEQG 142
           + MNL+ A+  AL  +L  DP + +FGEDV FGGVFRCT GL D+YGKDRVFNTPLCEQG
Sbjct: 69  QKMNLFQAVTSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQG 128

Query: 143 IVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGA 202
           IVGFGIG+A  G  AIAEIQFADYI+PAFDQIVNEAAK+RYRSG+ FNCG LTIR+P+G 
Sbjct: 129 IVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRSPWGC 188

Query: 203 VGHGGHYHSQSPEAFFCH-----VVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAV 262
           VGHG  YHSQSPEAFF H     VV+PRSP+QAKGLLLSCI D+NP +FFEPK LYR AV
Sbjct: 189 VGHGALYHSQSPEAFFAHCPGIKVVVPRSPFQAKGLLLSCIEDKNPCIFFEPKILYRAAV 248

Query: 263 EEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLSVM-EQACIDAEKDGISCELIDLRTLI 322
           E+VP   + +PLS+AEVI+EGSD+TLV WG Q+ V+ E A +  EK G+SCE+IDLRT++
Sbjct: 249 EQVPVEPYNIPLSQAEVIQEGSDVTLVAWGTQVHVIREVAAMAQEKLGVSCEVIDLRTIL 308

Query: 323 PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPVSRVCGLDTPFP 382
           PWD +TV  SV KTGRLL+SHEAP+TGGF +EIS+++ E CFL LEAP+SRVCG DTPFP
Sbjct: 309 PWDVDTVCKSVIKTGRLLVSHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFP 368

Query: 383 LVFEPFYMPTKNKILDAIKATVKY 401
            +FEPFY+P K K  DA++  + Y
Sbjct: 369 HIFEPFYIPDKWKCYDALRKMINY 392

[Close]

BLAST of Carg12919 vs. ExPASy Swiss-Prot
Match: P35738 (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Rattus norvegicus OX=10116 GN=Bckdhb PE=1 SV=3)

HSP 1 Score: 464.2 bits (1193), Expect = 1.5e-129
Identity = 228/353 (64.59%), Postives = 274/353 (77.62%), Query Frame = 0


Query: 58  PKLSNSICRRR---FSASSDGNLTSHG-GKSMNLYSAINQALHIALETDPRSYVFGEDVG 117
           P + ++  +RR   F+   D     +G  + MNL+ +I  AL  +L  DP + +FGEDV 
Sbjct: 38  PAVDDASQKRRVAHFTFQPDPESLQYGQTQKMNLFQSITSALDNSLAKDPTAVIFGEDVA 97

Query: 118 FGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQ 177
           FGGVFRCT GL D+YGKDRVFNTPLCEQGIVGFGIG+A  G  AIAEIQFADYI+PAFDQ
Sbjct: 98  FGGVFRCTVGLRDKYGKDRVFNTPLCEQGIVGFGIGIAVTGATAIAEIQFADYIFPAFDQ 157

Query: 178 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPY 237
           IVNEAAK+RYRSG+ FNCG LTIRAP+G VGHG  YHSQSPEAFF H     VVIPRSP+
Sbjct: 158 IVNEAAKYRYRSGDLFNCGSLTIRAPWGCVGHGALYHSQSPEAFFAHCPGIKVVIPRSPF 217

Query: 238 QAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGA 297
           QAKGLLLSCI D+NP +FFEPK LYR AVE+VP   + +PLS+AEVI+EGSD+TLV WG 
Sbjct: 218 QAKGLLLSCIEDKNPCIFFEPKILYRAAVEQVPVEPYKIPLSQAEVIQEGSDVTLVAWGT 277

Query: 298 QLSVM-EQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGA 357
           Q+ V+ E A +  EK G+SCE+IDLRT++PWD +TV  SV KTGRLLISHEAP+TGGF +
Sbjct: 278 QVHVIREVASMAQEKLGVSCEVIDLRTIVPWDVDTVCKSVIKTGRLLISHEAPLTGGFAS 337

Query: 358 EISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           EIS+++ E CFL LEAP+SRVCG DTPFP +FEPFY+P K K  DA++  + Y
Sbjct: 338 EISSTVQEECFLNLEAPISRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY 390

[Close]

BLAST of Carg12919 vs. ExPASy TrEMBL
Match: A0A6J1H8B9 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461465 PE=4 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 4.3e-199
Identity = 345/351 (98.29%), Postives = 345/351 (98.29%), Query Frame = 0


Query: 55  RRSPKLSNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 114
           RRSPKL NSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF
Sbjct: 6   RRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 65

Query: 115 GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 174
           GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI
Sbjct: 66  GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 125

Query: 175 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPYQ 234
           VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH     VVIPRSPYQ
Sbjct: 126 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQ 185

Query: 235 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ 294
           AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ
Sbjct: 186 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ 245

Query: 295 LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 354
           LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI
Sbjct: 246 LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 305

Query: 355 SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 306 SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

[Close]

BLAST of Carg12919 vs. ExPASy TrEMBL
Match: A0A6J1JGI1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111484908 PE=4 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 3.7e-195
Identity = 340/351 (96.87%), Postives = 343/351 (97.72%), Query Frame = 0


Query: 55  RRSPKLSNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 114
           RRSPKL +SICRR+FSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF
Sbjct: 6   RRSPKLLSSICRRQFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 65

Query: 115 GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 174
           GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI
Sbjct: 66  GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 125

Query: 175 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPYQ 234
           VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH     VVIPRSPYQ
Sbjct: 126 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQ 185

Query: 235 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ 294
           AKGLLLSCIRDQNPVVFFEPK LYRLAVEEVPE DFMLPLSEAEVIREGSDITLVGWGAQ
Sbjct: 186 AKGLLLSCIRDQNPVVFFEPKLLYRLAVEEVPEDDFMLPLSEAEVIREGSDITLVGWGAQ 245

Query: 295 LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 354
           LSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVTGGFGAEI
Sbjct: 246 LSVMEQACIDAEKDGISCELIDLRTLMPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 305

Query: 355 SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 306 SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

[Close]

BLAST of Carg12919 vs. ExPASy TrEMBL
Match: A0A1S3BQK1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103492412 PE=4 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 7.3e-191
Identity = 326/351 (92.88%), Postives = 339/351 (96.58%), Query Frame = 0


Query: 55  RRSPKLSNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 114
           RRS  + NS+CRR+FSAS DGNLT+H GKSMNLYSAINQALHIALETDPR+YVFGEDVGF
Sbjct: 6   RRSQTVLNSLCRRQFSASPDGNLTTHAGKSMNLYSAINQALHIALETDPRAYVFGEDVGF 65

Query: 115 GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 174
           GGVFRCTTGL DRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI
Sbjct: 66  GGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 125

Query: 175 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPYQ 234
           VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH     VVIPRSPYQ
Sbjct: 126 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQ 185

Query: 235 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ 294
           AKGLLLSCIRD+NPV+FFEPKWLYRLAVEEVPE DFMLPLS+AEVIREGSDITLVGWGAQ
Sbjct: 186 AKGLLLSCIRDENPVIFFEPKWLYRLAVEEVPEDDFMLPLSQAEVIREGSDITLVGWGAQ 245

Query: 295 LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 354
           LSVMEQAC+DAEK+GISCELIDLRTL+PWDKETVEASV+KTGRLLISHEAPVTGGFGAEI
Sbjct: 246 LSVMEQACVDAEKEGISCELIDLRTLLPWDKETVEASVRKTGRLLISHEAPVTGGFGAEI 305

Query: 355 SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           SASIVERCFLRLEAP+SRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 306 SASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

[Close]

BLAST of Carg12919 vs. ExPASy TrEMBL
Match: A0A6J1D329 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016561 PE=4 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 9.5e-191
Identity = 329/351 (93.73%), Postives = 338/351 (96.30%), Query Frame = 0


Query: 55  RRSPKLSNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 114
           RRS  + NSICRR+FS+SSDG+LT   GKSMNLYSAINQALHIALETDPRSYVFGEDVGF
Sbjct: 6   RRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 65

Query: 115 GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 174
           GGVFRCTTGL +RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI
Sbjct: 66  GGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 125

Query: 175 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPYQ 234
           VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH     VVIPRSPYQ
Sbjct: 126 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQ 185

Query: 235 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQ 294
           AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREG+DITLVGWGAQ
Sbjct: 186 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQ 245

Query: 295 LSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 354
           LSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVTGGFGAEI
Sbjct: 246 LSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 305

Query: 355 SASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           SASIVERCFLRLEAP+SRVCGLDTPFPLVFEP YMPTKNKILDAIK+TVKY
Sbjct: 306 SASIVERCFLRLEAPISRVCGLDTPFPLVFEPXYMPTKNKILDAIKSTVKY 356

[Close]

BLAST of Carg12919 vs. ExPASy TrEMBL
Match: A0A660KMJ8 (Transket_pyr domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_009543 PE=4 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 4.2e-178
Identity = 307/349 (87.97%), Postives = 323/349 (92.55%), Query Frame = 0


Query: 60  LSNSIC---RRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGG 119
           +  SIC   RR FS S D  L     KS NLYSAINQALHIALETDPR+YVFGEDVGFGG
Sbjct: 9   IGRSICRVSRRGFSTSCDDKLVQPSSKSTNLYSAINQALHIALETDPRAYVFGEDVGFGG 68

Query: 120 VFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVN 179
           VFRCTTGL DR+GK RVFNTPLCEQGI+GFGIGLAAMGNRA+AEIQFADYIYPAFDQIVN
Sbjct: 69  VFRCTTGLADRFGKSRVFNTPLCEQGIIGFGIGLAAMGNRAVAEIQFADYIYPAFDQIVN 128

Query: 180 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPYQAK 239
           EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH     VVIPRSP QAK
Sbjct: 129 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPQQAK 188

Query: 240 GLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLS 299
           GLLLSCIRD NPVVFFEPKWLYRLAVEEVPE+D+MLPLSEAEVIREGSDITLVGWGAQLS
Sbjct: 189 GLLLSCIRDPNPVVFFEPKWLYRLAVEEVPEYDYMLPLSEAEVIREGSDITLVGWGAQLS 248

Query: 300 VMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 359
           VMEQAC+DAEK+GISCELIDL+TLIPWDKET+E+SV+KTGRLLISHEAPVTGGFGAEISA
Sbjct: 249 VMEQACLDAEKEGISCELIDLKTLIPWDKETIESSVRKTGRLLISHEAPVTGGFGAEISA 308

Query: 360 SIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           SIVERCFLRLEAPV+RVCGLDTPFPLVFEPFYMPTKNKILDAIK+T+ Y
Sbjct: 309 SIVERCFLRLEAPVARVCGLDTPFPLVFEPFYMPTKNKILDAIKSTINY 357

[Close]

BLAST of Carg12919 vs. TAIR 10
Match: AT1G55510.1 (branched-chain alpha-keto acid decarboxylase E1 beta subunit )

HSP 1 Score: 617.8 bits (1592), Expect = 5.9e-177
Identity = 299/348 (85.92%), Postives = 322/348 (92.53%), Query Frame = 0


Query: 60  LSNSICRRRFSASSDG--NLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGV 119
           L  S  +  F + S G   +++  GK +NLYSAINQALHIAL+TDPRSYVFGEDVGFGGV
Sbjct: 5   LGRSCRKLSFPSLSHGARRVSTETGKPLNLYSAINQALHIALDTDPRSYVFGEDVGFGGV 64

Query: 120 FRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNE 179
           FRCTTGL +R+GK+RVFNTPLCEQGIVGFGIGLAAMGNRAI EIQFADYIYPAFDQIVNE
Sbjct: 65  FRCTTGLAERFGKNRVFNTPLCEQGIVGFGIGLAAMGNRAIVEIQFADYIYPAFDQIVNE 124

Query: 180 AAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPYQAKG 239
           AAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH     VVIPRSP +AKG
Sbjct: 125 AAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAKG 184

Query: 240 LLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLSV 299
           LLLSCIRD NPVVFFEPKWLYR AVEEVPEHD+M+PLSEAEVIREG+DITLVGWGAQL+V
Sbjct: 185 LLLSCIRDPNPVVFFEPKWLYRQAVEEVPEHDYMIPLSEAEVIREGNDITLVGWGAQLTV 244

Query: 300 MEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISAS 359
           MEQAC+DAEK+GISCELIDL+TL+PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA+
Sbjct: 245 MEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISAT 304

Query: 360 IVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           I+ERCFL+LEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIK+TV Y
Sbjct: 305 ILERCFLKLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY 352

[Close]

BLAST of Carg12919 vs. TAIR 10
Match: AT3G13450.1 (Transketolase family protein )

HSP 1 Score: 605.9 bits (1561), Expect = 2.3e-173
Identity = 295/352 (83.81%), Postives = 315/352 (89.49%), Query Frame = 0


Query: 67  RRFSASSDGNLTSHG-------------GKSMNLYSAINQALHIALETDPRSYVFGEDVG 126
           RRF   S   ++ HG             GKSMNLYSAINQALHIALETDPRSYVFGEDVG
Sbjct: 7   RRFCRGSSFPVSGHGYRMLSTVENVSESGKSMNLYSAINQALHIALETDPRSYVFGEDVG 66

Query: 127 FGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQ 186
           FGGVFRCTTGL +R+GK RVFNTPLCEQGIVGFGIGLAAMGNR IAEIQFADYI+PAFDQ
Sbjct: 67  FGGVFRCTTGLAERFGKSRVFNTPLCEQGIVGFGIGLAAMGNRVIAEIQFADYIFPAFDQ 126

Query: 187 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH-----VVIPRSPY 246
           IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH     VVIPRSP 
Sbjct: 127 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPR 186

Query: 247 QAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGA 306
           +AKGLLLS IRD NPVVFFEPKWLYR AVE+VPE D+M+PLSEAEV+REGSDITLVGWGA
Sbjct: 187 EAKGLLLSSIRDPNPVVFFEPKWLYRQAVEDVPEDDYMIPLSEAEVMREGSDITLVGWGA 246

Query: 307 QLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAE 366
           QL++MEQAC+DAE +GISCELIDL+TLIPWDKE VE SV+KTGRLLISHEAPVTGGFGAE
Sbjct: 247 QLTIMEQACLDAENEGISCELIDLKTLIPWDKEIVETSVRKTGRLLISHEAPVTGGFGAE 306

Query: 367 ISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 401
           I+A+IVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAI++TV Y
Sbjct: 307 IAATIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIRSTVNY 358

[Close]

BLAST of Carg12919 vs. TAIR 10
Match: AT5G50850.1 (Transketolase family protein )

HSP 1 Score: 214.9 bits (546), Expect = 1.2e-55
Identity = 126/346 (36.42%), Postives = 190/346 (54.91%), Query Frame = 0


Query: 61  SNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVG-FGGVFR 120
           ++++ R RF+  S  +  + G K M +  A+N A+   +  DP+ +V GE+VG + G ++
Sbjct: 13  ASTLRRTRFALVSARSYAA-GAKEMTVRDALNSAIDEEMSADPKVFVMGEEVGQYQGAYK 72

Query: 121 CTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAA 180
            T GL+++YG +RV++TP+ E G  G G+G A  G + + E    ++   A D I+N AA
Sbjct: 73  ITKGLLEKYGPERVYDTPITEAGFTGIGVGAAYAGLKPVVEFMTFNFSMQAIDHIINSAA 132

Query: 181 KFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFC-----HVVIPRSPYQAKGLL 240
           K  Y S  Q N   +  R P GA    G  HSQ   A++       V+ P S   A+GLL
Sbjct: 133 KSNYMSAGQINV-PIVFRGPNGAAAGVGAQHSQCYAAWYASVPGLKVLAPYSAEDARGLL 192

Query: 241 LSCIRDQNPVVFFEPKWLYRLAV---EEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLS 300
            + IRD +PVVF E + LY  +    EE  +  F LP+ +A++ REG D+T+V +   + 
Sbjct: 193 KAAIRDPDPVVFLENELLYGESFPISEEALDSSFCLPIGKAKIEREGKDVTIVTFSKMVG 252

Query: 301 VMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 360
              +A     ++GIS E+I+LR++ P D+ T+ ASV+KT RL+   E     G  AEI A
Sbjct: 253 FALKAAEKLAEEGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGFPQHGVCAEICA 312

Query: 361 SIVERCFLRLEAPVSRVCGLDTPFPLV--FEPFYMPTKNKILDAIK 396
           S+VE  F  L+APV R+ G D P P     E   +P    I+ A K
Sbjct: 313 SVVEESFSYLDAPVERIAGADVPMPYAANLERLALPQIEDIVRASK 356

[Close]

BLAST of Carg12919 vs. TAIR 10
Match: AT1G30120.1 (pyruvate dehydrogenase E1 beta )

HSP 1 Score: 203.0 bits (515), Expect = 4.6e-52
Identity = 117/312 (37.50%), Postives = 170/312 (54.49%), Query Frame = 0


Query: 71  ASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVG-FGGVFRCTTGLVDRYG 130
           A+ + + ++  G  + L+ A+ + L   ++ DP   V GEDVG +GG ++ T GL D++G
Sbjct: 72  ATKEADTSASTGHELLLFEALQEGLEEEMDRDPHVCVMGEDVGHYGGSYKVTKGLADKFG 131

Query: 131 KDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQF 190
             RV +TP+CE    G GIG A  G R + E     ++  AF+QI N      Y SG QF
Sbjct: 132 DLRVLDTPICENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFNQISNNCGMLHYTSGGQF 191

Query: 191 NCGGLTIRAPYGAVGHGGHYHSQSPEAFF-----CHVVIPRSPYQAKGLLLSCIRDQNPV 250
               + IR P G     G  HSQ  E++F       +V   +PY AKGL+ + IR +NPV
Sbjct: 192 TI-PVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAAIRSENPV 251

Query: 251 VFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLSVMEQACIDAEKDG 310
           + FE   LY L  E++P+ D++  L EAE++R G  IT++ +      + QA       G
Sbjct: 252 ILFEHVLLYNLK-EKIPDEDYVCNLEEAEMVRPGEHITILTYSRMRYHVMQAAKTLVNKG 311

Query: 311 ISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAP 370
              E+ID+R+L P+D  T+  SVKKT R+LI  E   TGG GA ++A+I E     L+AP
Sbjct: 312 YDPEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGASLTAAINENFHDYLDAP 371

Query: 371 VSRVCGLDTPFP 377
           V  +   D P P
Sbjct: 372 VMCLSSQDVPTP 381

[Close]

BLAST of Carg12919 vs. TAIR 10
Match: AT2G34590.1 (Transketolase family protein )

HSP 1 Score: 202.6 bits (514), Expect = 5.9e-52
Identity = 121/328 (36.89%), Postives = 177/328 (53.96%), Query Frame = 0


Query: 55  RRSPKLSNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVG- 114
           R  P + N++  +  +A+S  + +S  G  + L+ A+ + L   ++ DP   V GEDVG 
Sbjct: 58  RSEPLIPNAVTTKADTAAS--STSSKPGHELLLFEALQEGLEEEMDRDPHVCVMGEDVGH 117

Query: 115 FGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQ 174
           +GG ++ T GL D++G  RV +TP+CE    G GIG A  G R + E     ++  AF+Q
Sbjct: 118 YGGSYKVTKGLADKFGDLRVLDTPICENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFNQ 177

Query: 175 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFF-----CHVVIPRSPY 234
           I N      Y SG QF    + IR P G     G  HSQ  E++F       +V   +PY
Sbjct: 178 ISNNCGMLHYTSGGQFTI-PVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTPY 237

Query: 235 QAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGA 294
            AKGL+ + IR +NPV+ FE   LY L  E +P+ +++  L EAE++R G  IT++ +  
Sbjct: 238 NAKGLMKAAIRSENPVILFEHVLLYNLK-ESIPDEEYICNLEEAEMVRPGEHITILTYSR 297

Query: 295 QLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAE 354
               + QA       G   E+ID+R+L P+D  T+  SVKKT R+LI  E   TGG GA 
Sbjct: 298 MRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLYTIGNSVKKTHRVLIVEECMRTGGIGAS 357

Query: 355 ISASIVERCFLRLEAPVSRVCGLDTPFP 377
           ++A+I E     L+APV  +   D P P
Sbjct: 358 LTAAINENFHDYLDAPVMCLSSQDVPTP 381

The following BLAST results are available for this feature:

BLAST of Carg12919 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023738.1	2.1e-232	100.00	2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita argyrosp...	[more]
XP_022960762.1	8.8e-199	98.29	2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita moschata...	[more]
XP_023515905.1	2.6e-198	98.01	2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial isoform X1 [Cucurbi...	[more]
XP_022987320.1	7.7e-195	96.87	2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita maxima]	[more]
XP_008450996.1	1.5e-190	92.88	PREDICTED: 2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucumis...	[more]

BLAST of Carg12919 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SAV3	8.3e-176	85.92	2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Arabidopsis thal...	[more]
Q9LDY2	3.3e-172	83.81	2-oxoisovalerate dehydrogenase subunit beta 2, mitochondrial OS=Arabidopsis thal...	[more]
Q6P3A8	5.3e-130	68.52	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Mus musculus OX=10...	[more]
P21839	1.5e-129	67.59	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Bos taurus OX=9913...	[more]
P35738	1.5e-129	64.59	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Rattus norvegicus ...	[more]

BLAST of Carg12919 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H8B9	4.3e-199	98.29	2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita moscha...	[more]
A0A6J1JGI1	3.7e-195	96.87	2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita maxima...	[more]
A0A1S3BQK1	7.3e-191	92.88	2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucumis melo OX=...	[more]
A0A6J1D329	9.5e-191	93.73	2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Momordica charan...	[more]
A0A660KMJ8	4.2e-178	87.97	Transket_pyr domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_0...	[more]

BLAST of Carg12919 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55510.1	5.9e-177	85.92	branched-chain alpha-keto acid decarboxylase E1 beta subunit	[more]
AT3G13450.1	2.3e-173	83.81	Transketolase family protein	[more]
AT5G50850.1	1.2e-55	36.42	Transketolase family protein	[more]
AT1G30120.1	4.6e-52	37.50	pyruvate dehydrogenase E1 beta	[more]
AT2G34590.1	5.9e-52	36.89	Transketolase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005475	Transketolase-like, pyrimidine-binding domain	SMART	SM00861	Transket_pyr_3	coord: 85..255 e-value: 6.5E-58 score: 208.4
IPR005475	Transketolase-like, pyrimidine-binding domain	PFAM	PF02779	Transket_pyr	coord: 85..254 e-value: 4.7E-38 score: 130.6
IPR009014	Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain II	GENE3D	3.40.50.920		coord: 269..399 e-value: 1.3E-48 score: 166.3
IPR009014	Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain II	SUPERFAMILY	52922	TK C-terminal domain-like	coord: 264..400
IPR033248	Transketolase, C-terminal domain	PFAM	PF02780	Transketolase_C	coord: 271..390 e-value: 1.1E-35 score: 122.2
None	No IPR available	GENE3D	3.40.50.970		coord: 82..268 e-value: 1.1E-87 score: 294.2
None	No IPR available	PANTHER	PTHR42980	2-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA-RELATED	coord: 73..400
None	No IPR available	CDD	cd07036	TPP_PYR_E1-PDHc-beta_like	coord: 90..250 e-value: 1.44462E-92 score: 273.585
IPR029061	Thiamin diphosphate-binding fold	SUPERFAMILY	52518	Thiamin diphosphate-binding fold (THDP-binding)	coord: 83..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12919-RA	Carg12919-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009083	branched-chain amino acid catabolic process
biological_process	GO:0007584	response to nutrient
cellular_component	GO:0005947	mitochondrial alpha-ketoglutarate dehydrogenase complex
molecular_function	GO:0003824	catalytic activity