Carg12448 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg12448 vs. NCBI nr
Match: KAG7017196.1 (ddlA [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2163.3 bits (5604), Expect = 0.0e+00
Identity = 1081/1081 (100.00%), Postives = 1081/1081 (100.00%), Query Frame = 0

Query: 1    MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60
            MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM
Sbjct: 1    MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60

Query: 61   VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120
            VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV
Sbjct: 61   VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120

Query: 121  SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180
            SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH
Sbjct: 121  SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180

Query: 181  GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS 240
            GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS
Sbjct: 181  GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS 240

Query: 241  ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300
            ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV
Sbjct: 241  ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300

Query: 301  EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ 360
            EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ
Sbjct: 301  EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ 360

Query: 361  QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420
            QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA
Sbjct: 361  QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420

Query: 421  SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480
            SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM
Sbjct: 421  SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480

Query: 481  PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540
            PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL
Sbjct: 481  PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540

Query: 541  APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600
            APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR
Sbjct: 541  APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600

Query: 601  ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAGVVSNRQVPIL 660
            ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAGVVSNRQVPIL
Sbjct: 601  ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAGVVSNRQVPIL 660

Query: 661  GMCSSSPLYECNYNGIRKLTNIMHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA 720
            GMCSSSPLYECNYNGIRKLTNIMHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA
Sbjct: 661  GMCSSSPLYECNYNGIRKLTNIMHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA 720

Query: 721  TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV 780
            TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV
Sbjct: 721  TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV 780

Query: 781  ARLCCADDLAVYVKALEDCLVRIPSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHGMIE 840
            ARLCCADDLAVYVKALEDCLVRIPSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHGMIE
Sbjct: 781  ARLCCADDLAVYVKALEDCLVRIPSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHGMIE 840

Query: 841  MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP 900
            MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP
Sbjct: 841  MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP 900

Query: 901  SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI 960
            SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI
Sbjct: 901  SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI 960

Query: 961  DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQRNHDAVEDYWDWECPTLVNLVEDHGFISE 1020
            DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQRNHDAVEDYWDWECPTLVNLVEDHGFISE
Sbjct: 961  DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQRNHDAVEDYWDWECPTLVNLVEDHGFISE 1020

Query: 1021 EYYLHWAPERSRASIKRRLFESSMLRAYSVLCSREALAETPPVYPHQFFRRLLDLALERS 1080
            EYYLHWAPERSRASIKRRLFESSMLRAYSVLCSREALAETPPVYPHQFFRRLLDLALERS
Sbjct: 1021 EYYLHWAPERSRASIKRRLFESSMLRAYSVLCSREALAETPPVYPHQFFRRLLDLALERS 1080

Query: 1081 P 1082
            P
Sbjct: 1081 P 1081

BLAST of Carg12448 vs. NCBI nr
Match: XP_022934689.1 (uncharacterized protein LOC111441803 [Cucurbita moschata] >XP_022934690.1 uncharacterized protein LOC111441803 [Cucurbita moschata] >XP_022934691.1 uncharacterized protein LOC111441803 [Cucurbita moschata] >XP_022934692.1 uncharacterized protein LOC111441803 [Cucurbita moschata] >KAG6580439.1 hypothetical protein SDJN03_20441, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1844.7 bits (4777), Expect = 0.0e+00
Identity = 958/1081 (88.62%), Postives = 959/1081 (88.71%), Query Frame = 0

Query: 1    MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60
            MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM
Sbjct: 1    MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60

Query: 61   VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120
            VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV
Sbjct: 61   VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120

Query: 121  SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180
            SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH
Sbjct: 121  SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180

Query: 181  GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS 240
            GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS
Sbjct: 181  GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS 240

Query: 241  ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300
            ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV
Sbjct: 241  ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300

Query: 301  EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ 360
            EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ
Sbjct: 301  EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ 360

Query: 361  QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420
            QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA
Sbjct: 361  QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420

Query: 421  SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480
            SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM
Sbjct: 421  SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480

Query: 481  PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540
            PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL
Sbjct: 481  PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540

Query: 541  APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600
            APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR
Sbjct: 541  APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600

Query: 601  ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAGVVSNRQVPIL 660
            ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIA           
Sbjct: 601  ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIA----------- 660

Query: 661  GMCSSSPLYECNYNGIRKLTNIMHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA 720
                                  +HGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA
Sbjct: 661  ----------------------VHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA 720

Query: 721  TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV 780
            TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV
Sbjct: 721  TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV 780

Query: 781  ARLCCADDLAVYVKALEDCLVRIPSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHGMIE 840
            ARLCCADDLAVYVKALEDCLVRIPSNSLSR                        AHGMIE
Sbjct: 781  ARLCCADDLAVYVKALEDCLVRIPSNSLSR------------------------AHGMIE 840

Query: 841  MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP 900
            MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP
Sbjct: 841  MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP 900

Query: 901  SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI 960
            SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI
Sbjct: 901  SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI 960

Query: 961  DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQRNHDAVEDYWDWECPTLVNLVEDHGFISE 1020
            DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQ                             
Sbjct: 961  DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQ----------------------------- 960

Query: 1021 EYYLHWAPERSRASIKRRLFESSMLRAYSVLCSREALAETPPVYPHQFFRRLLDLALERS 1080
                                               ALAETPPVYPHQFFRRLLDLA ERS
Sbjct: 1021 -----------------------------------ALAETPPVYPHQFFRRLLDLAWERS 960

Query: 1081 P 1082
            P
Sbjct: 1081 P 960

BLAST of Carg12448 vs. NCBI nr
Match: XP_023528547.1 (uncharacterized protein LOC111791436 [Cucurbita pepo subsp. pepo] >XP_023528548.1 uncharacterized protein LOC111791436 [Cucurbita pepo subsp. pepo] >XP_023528549.1 uncharacterized protein LOC111791436 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1837.4 bits (4758), Expect = 0.0e+00
Identity = 954/1081 (88.25%), Postives = 957/1081 (88.53%), Query Frame = 0

Query: 1    MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60
            MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFL LSLDLHNHA RLQSPKIRVSRAAAKM
Sbjct: 1    MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLGLSLDLHNHAPRLQSPKIRVSRAAAKM 60

Query: 61   VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120
            VVKDGVAEMA TEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV
Sbjct: 61   VVKDGVAEMAATEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120

Query: 121  SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180
            SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH
Sbjct: 121  SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180

Query: 181  GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS 240
            GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNA+LELDRLGFITVPNFLVQAGSVS
Sbjct: 181  GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNASLELDRLGFITVPNFLVQAGSVS 240

Query: 241  ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300
            ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV
Sbjct: 241  ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300

Query: 301  EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ 360
            EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVEL+SHAWADAGEKDAIFNYRRKYLPTQ
Sbjct: 301  EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELQSHAWADAGEKDAIFNYRRKYLPTQ 360

Query: 361  QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420
            QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA
Sbjct: 361  QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420

Query: 421  SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480
            SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM
Sbjct: 421  SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480

Query: 481  PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540
            PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL
Sbjct: 481  PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540

Query: 541  APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600
            APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR
Sbjct: 541  APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600

Query: 601  ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAGVVSNRQVPIL 660
            ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIA           
Sbjct: 601  ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIA----------- 660

Query: 661  GMCSSSPLYECNYNGIRKLTNIMHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA 720
                                  +HGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA
Sbjct: 661  ----------------------VHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA 720

Query: 721  TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV 780
            TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV
Sbjct: 721  TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV 780

Query: 781  ARLCCADDLAVYVKALEDCLVRIPSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHGMIE 840
            ARLCCADDLAVYVKALEDCLVRIPSNSLSR                        AHGMIE
Sbjct: 781  ARLCCADDLAVYVKALEDCLVRIPSNSLSR------------------------AHGMIE 840

Query: 841  MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP 900
            MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP
Sbjct: 841  MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP 900

Query: 901  SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI 960
            SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI
Sbjct: 901  SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI 960

Query: 961  DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQRNHDAVEDYWDWECPTLVNLVEDHGFISE 1020
            DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQ                             
Sbjct: 961  DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQ----------------------------- 960

Query: 1021 EYYLHWAPERSRASIKRRLFESSMLRAYSVLCSREALAETPPVYPHQFFRRLLDLALERS 1080
                                               ALAETPPVYPHQFFRRLLDLALERS
Sbjct: 1021 -----------------------------------ALAETPPVYPHQFFRRLLDLALERS 960

Query: 1081 P 1082
            P
Sbjct: 1081 P 960

BLAST of Carg12448 vs. NCBI nr
Match: XP_022982716.1 (uncharacterized protein LOC111481502 [Cucurbita maxima] >XP_022982717.1 uncharacterized protein LOC111481502 [Cucurbita maxima] >XP_022982718.1 uncharacterized protein LOC111481502 [Cucurbita maxima])

HSP 1 Score: 1815.4 bits (4701), Expect = 0.0e+00
Identity = 942/1081 (87.14%), Postives = 950/1081 (87.88%), Query Frame = 0

Query: 1    MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60
            MAH ANCSTC VLCKDI+ RASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM
Sbjct: 1    MAHKANCSTCKVLCKDIEPRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60

Query: 61   VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120
            VVKDGVAEMA TEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV
Sbjct: 61   VVKDGVAEMAATEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120

Query: 121  SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180
            SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH
Sbjct: 121  SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180

Query: 181  GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS 240
            GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNA+LELDRLGFITVPNFLVQAGSVS
Sbjct: 181  GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNASLELDRLGFITVPNFLVQAGSVS 240

Query: 241  ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300
            ESDLSKWFLRNQLDLSSGKVVVKP RAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV
Sbjct: 241  ESDLSKWFLRNQLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300

Query: 301  EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ 360
            EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVEL+SH WADAGEKDAIFNYRRKYLPTQ
Sbjct: 301  EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELQSHGWADAGEKDAIFNYRRKYLPTQ 360

Query: 361  QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420
            QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA
Sbjct: 361  QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420

Query: 421  SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480
            SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM
Sbjct: 421  SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480

Query: 481  PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540
            PRRSISSQR+QAYSSTESNQKVF+I GGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL
Sbjct: 481  PRRSISSQRSQAYSSTESNQKVFLIFGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540

Query: 541  APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600
            APSIEQTSSVDLDKNEADMTS+TVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR
Sbjct: 541  APSIEQTSSVDLDKNEADMTSKTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600

Query: 601  ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAGVVSNRQVPIL 660
            ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIA           
Sbjct: 601  ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIA----------- 660

Query: 661  GMCSSSPLYECNYNGIRKLTNIMHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA 720
                                  +HGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA
Sbjct: 661  ----------------------VHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA 720

Query: 721  TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV 780
            TSLALNHLSDMGVLTIKKDARRKDELL+IPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV
Sbjct: 721  TSLALNHLSDMGVLTIKKDARRKDELLNIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV 780

Query: 781  ARLCCADDLAVYVKALEDCLVRIPSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHGMIE 840
            ARLCCADDLAVY KALE CLVRIPSNSLSR                        AHGMIE
Sbjct: 781  ARLCCADDLAVYAKALEYCLVRIPSNSLSR------------------------AHGMIE 840

Query: 841  MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP 900
            MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP
Sbjct: 841  MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP 900

Query: 901  SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI 960
            SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIAN LQLEGFSRI
Sbjct: 901  SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANALQLEGFSRI 960

Query: 961  DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQRNHDAVEDYWDWECPTLVNLVEDHGFISE 1020
            DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQ                             
Sbjct: 961  DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQ----------------------------- 960

Query: 1021 EYYLHWAPERSRASIKRRLFESSMLRAYSVLCSREALAETPPVYPHQFFRRLLDLALERS 1080
                                               ALAETPPVYPHQFFRRLLDLALERS
Sbjct: 1021 -----------------------------------ALAETPPVYPHQFFRRLLDLALERS 960

Query: 1081 P 1082
            P
Sbjct: 1081 P 960

BLAST of Carg12448 vs. NCBI nr
Match: XP_038903726.1 (uncharacterized protein LOC120090245 isoform X1 [Benincasa hispida] >XP_038903727.1 uncharacterized protein LOC120090245 isoform X1 [Benincasa hispida] >XP_038903728.1 uncharacterized protein LOC120090245 isoform X1 [Benincasa hispida] >XP_038903729.1 uncharacterized protein LOC120090245 isoform X1 [Benincasa hispida])

HSP 1 Score: 1677.5 bits (4343), Expect = 0.0e+00
Identity = 874/1082 (80.78%), Postives = 916/1082 (84.66%), Query Frame = 0

Query: 1    MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHA--RRLQSPKIRVSRAAA 60
            MA+I NCSTC+V  K +KLRAS F PTL SSFLRLSLDL+N+   RRLQSPKI + R+AA
Sbjct: 1    MAYITNCSTCDVSSKALKLRASTFAPTLPSSFLRLSLDLNNYTRRRRLQSPKIHLPRSAA 60

Query: 61   KMVVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDL 120
            K++ KDGVA+MA T +ERE+ S KC ALRVGLICGGPSAERGISLNSARSVLDHIQGDDL
Sbjct: 61   KVLAKDGVAQMAVTGMERERQSEKCGALRVGLICGGPSAERGISLNSARSVLDHIQGDDL 120

Query: 121  QVSCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPV 180
            QVSCYYID ELNAYAISSAQVYSNTPADFDFKLKSLARGF SLT+FAEHLSTSVDIVFPV
Sbjct: 121  QVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFLSLTEFAEHLSTSVDIVFPV 180

Query: 181  IHGRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGS 240
            IHGRFGEDGGIQELLETHNIPFVGTGST+C+HAFDKYNA+LELDRLGFITVPNFLVQAG 
Sbjct: 181  IHGRFGEDGGIQELLETHNIPFVGTGSTQCSHAFDKYNASLELDRLGFITVPNFLVQAGG 240

Query: 241  VSESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKV 300
            VSES+LSKWFL NQLDLSSGKVVVKP RAGSSIGVSVAYGVVDSLKKANEIIS EIDDKV
Sbjct: 241  VSESELSKWFLSNQLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISMEIDDKV 300

Query: 301  LVEVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLP 360
            LVEVFLE GSEFTAIVLDVGSGSVCHPVVLLPTEVE++S   ADAGEKDAIFNYRRKYLP
Sbjct: 301  LVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVEIQSLGLADAGEKDAIFNYRRKYLP 360

Query: 361  TQQVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGR 420
            TQQVAYHTPPRFPIDVIE+IREGASLLFKGLGLCDFARIDGW+LPN SH+SS S GKFGR
Sbjct: 361  TQQVAYHTPPRFPIDVIETIREGASLLFKGLGLCDFARIDGWYLPNFSHESSCSMGKFGR 420

Query: 421  TASGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSG 480
            T SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSG
Sbjct: 421  TKSGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSG 480

Query: 481  NMPRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPC 540
            N PRRSISSQR+QAYSS ES +KVFVI GGDTSERQVSLMSGTNVWLNLQAFD+LEVTPC
Sbjct: 481  NTPRRSISSQRSQAYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQAFDELEVTPC 540

Query: 541  LLAPSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSH 600
            LLAPSIEQTS VDLDKNEA++T +TVWSLPYSLVLRHTTEEVLAACIEA EPTRAALTSH
Sbjct: 541  LLAPSIEQTSGVDLDKNEANLTFKTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSH 600

Query: 601  LRELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAGVVSNRQVP 660
            LRELVVTDLEEGLKKHSWF+GFDIKDELPV+FSLEQWIKKAKEVDATVFIA         
Sbjct: 601  LRELVVTDLEEGLKKHSWFSGFDIKDELPVRFSLEQWIKKAKEVDATVFIA--------- 660

Query: 661  ILGMCSSSPLYECNYNGIRKLTNIMHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDK 720
                                    +HGGIGE+G LQSLLEA+GVPYTGPGVAASNICMDK
Sbjct: 661  ------------------------VHGGIGEDGTLQSLLEAQGVPYTGPGVAASNICMDK 720

Query: 721  VATSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCST 780
            V+TSLALNHLS+MGVLTIKKD RRK+ LLHIP LN+WHDLTCKLQCQSLCVKPARDGCST
Sbjct: 721  VSTSLALNHLSEMGVLTIKKDERRKEYLLHIPILNVWHDLTCKLQCQSLCVKPARDGCST 780

Query: 781  GVARLCCADDLAVYVKALEDCLVRIPSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHGM 840
            GVARLCCADDLAVYVKALE+CLVRIPSNSLS+                        AHGM
Sbjct: 781  GVARLCCADDLAVYVKALENCLVRIPSNSLSK------------------------AHGM 840

Query: 841  IEMPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSL 900
            IEMPKPPPELLIFEPFIETDEIIVSSK T+ESERLLWKGQSRWVEITVGVVGTRGSMHSL
Sbjct: 841  IEMPKPPPELLIFEPFIETDEIIVSSKSTDESERLLWKGQSRWVEITVGVVGTRGSMHSL 900

Query: 901  SPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFS 960
            SPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIAN LQLEGFS
Sbjct: 901  SPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANALQLEGFS 960

Query: 961  RIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQRNHDAVEDYWDWECPTLVNLVEDHGFI 1020
            RIDAFV+VDSG+VLVIEVNTVPGMTPSTVLIHQ                           
Sbjct: 961  RIDAFVDVDSGKVLVIEVNTVPGMTPSTVLIHQ--------------------------- 961

Query: 1021 SEEYYLHWAPERSRASIKRRLFESSMLRAYSVLCSREALAETPPVYPHQFFRRLLDLALE 1080
                                                 ALAETPPVYPHQFFRRLLDLALE
Sbjct: 1021 -------------------------------------ALAETPPVYPHQFFRRLLDLALE 961

BLAST of Carg12448 vs. ExPASy Swiss-Prot
Match: Q7NV72 (D-alanine--D-alanine ligase A OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) OX=243365 GN=ddlA PE=3 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 2.5e-25
Identity = 105/385 (27.27%), Postives = 151/385 (39.22%), Query Frame = 0

Query: 86  LRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDPELNAYAISSAQVYSNTPA 145
           +RVGLI GG S+E  +SL SAR++L  I G+  +VS   +D +   +A  ++    N   
Sbjct: 4   IRVGLIFGGQSSEHEVSLQSARNILQAIDGERFEVSLIGVDKQGRWHASQASNFLLNADD 63

Query: 146 DFDFKLKSLARGFRSL---------TDFAEHLSTSVDIVFPVIHGRFGEDGGIQELLETH 205
                L+        +         T    H    +D+ FP++HG  GEDG +Q LL   
Sbjct: 64  PGRIALRESGENLALVPGECSGQLQTAANAHPLAQIDVAFPIVHGTLGEDGSLQGLLRMA 123

Query: 206 NIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVSESDLSKWFLRNQLDLS 265
           NIPFVG G    A   DK  A   L   G    P   +     + +DLS   +  QL L 
Sbjct: 124 NIPFVGAGVLGSAVCMDKDVAKRLLRDAGLKVAPFVSLTRSKAAGADLSA--IVEQLGL- 183

Query: 266 SGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLVE-VFLERGSEFTAIVL 325
              + VKP   GSS+GVS      D     +E      D KVLVE   + R  E   +  
Sbjct: 184 --PLFVKPANQGSSVGVSKVKREADLRAALDEAF--RYDHKVLVEQAVIGREIECAVLGN 243

Query: 326 DVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQQVAYHTPPRFPIDVI 385
           +    S C  +VL                  D  + Y  KYL         P   P +  
Sbjct: 244 ERPRASGCGEIVL-----------------SDEFYAYDTKYLNEDGARVAVPADIPDEAC 303

Query: 386 ESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTASGTVVYTDINLISGM 445
           + IR  A   F+ L     AR+D +  P+                 G VV  ++N + G 
Sbjct: 304 QRIRGIAIEAFQALECSGMARVDVFLTPD-----------------GEVVINELNTLPGF 347

Query: 446 EQTSFLFQQASKVGFSHSNILRSII 461
              S   +     G S+  ++ ++I
Sbjct: 364 TNISMYPKLWQAAGMSYRELITALI 347

BLAST of Carg12448 vs. ExPASy Swiss-Prot
Match: Q0SNW3 (D-alanine--D-alanine ligase OS=Borrelia afzelii (strain PKo) OX=390236 GN=ddl PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 3.6e-24
Identity = 106/382 (27.75%), Postives = 168/382 (43.98%), Query Frame = 0

Query: 90  LICGGPSAERGISLNSARSV-LDHIQGDDLQVSCYYIDPELNAYAISSAQVYSNTPADFD 149
           LI GG S E  IS  SA SV L  +  +   +   YID     + +  +      P D D
Sbjct: 7   LIFGGVSFEHEISCKSAYSVYLALLDLNKYNIYSAYIDKCTGIWYLLDSVSDPPKPIDTD 66

Query: 150 -FKLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQELLETHNIPFVGTGSTE 209
              + SL  GF     F+ + +  +D+VFPV+HGR GEDG IQ +L+  +IP VG G   
Sbjct: 67  VLPIVSLLPGFGI---FSNNKNLEIDVVFPVVHGRTGEDGAIQGVLKVMDIPCVGAGIIG 126

Query: 210 CAHAFDKYNAALELDRLGFITVP--NFLVQAGSVSESDLSKWFLRNQLDLSSGKVVVKPV 269
            A + +KY   L L       VP   F     S+ + ++     RN  ++    V+VKP 
Sbjct: 127 SAISSNKYFCKLLLKSFNIPIVPFIGFRQYDYSLDKEEIK----RNVKEVLGYPVIVKPA 186

Query: 270 RAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLVEVFLERGSEFTAIVLDVGSGSVCHP 329
             GSSIG++VAY          E +  + D  +++E F+E      +I   +G+  +   
Sbjct: 187 VLGSSIGINVAYSENQIESCIEEAL--KYDLTIVIEKFIEAREIECSI---IGNEKM--- 246

Query: 330 VVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQQVAYHTPPRFPIDVIESIREGASLL 389
            +  P EV ++   + D   K ++       +P   + ++ P     + + SI+E A L+
Sbjct: 247 KIFSPGEVVVQDFIFYDYDAKYSV-------IPGNSIIFNIPAHLETNQLLSIKEYAFLV 306

Query: 390 FKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTASGTVVYTDINLISGMEQTSFLFQQA 449
           +K L L   AR+D                 F    SGT+   +IN I G    S   +  
Sbjct: 307 YKNLELRGMARVD----------------FFVEKKSGTIYLNEINTIPGFTDISMFSKMC 350

Query: 450 SKVGFSHSNILRSIIYHACLRY 468
           S  G    +++ ++I +A   Y
Sbjct: 367 SHDGLQFKDLIDNLIDYAFQSY 350

BLAST of Carg12448 vs. ExPASy Swiss-Prot
Match: Q03JC6 (D-alanine--D-alanine ligase OS=Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9) OX=322159 GN=ddl PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 3.6e-24
Identity = 107/374 (28.61%), Postives = 166/374 (44.39%), Query Frame = 0

Query: 90  LICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDPELNAYAISSAQVYSNTPADFDF 149
           L+ GG SAER +S+ SA SV+  +  +  +V  Y+I     +      Q +  TP D D 
Sbjct: 8   LLYGGRSAEREVSVLSAESVMRAVDYNAFEVKTYFI---TQSGDFIKTQEFIETPGD-DE 67

Query: 150 KLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQELLETHNIPFVGTGSTECA 209
           KL +      S       +     +VFPV+HG  GEDG IQ  LET  +P+VGT     +
Sbjct: 68  KLMTNDTVEASQAIKPSDIYEEDAVVFPVLHGPMGEDGSIQGFLETLKLPYVGTNVLSSS 127

Query: 210 HAFDKYNAALELDRLGFITVPNFLVQAGSVSESDLSKWFLRNQLDLSSGKVVVKPVRAGS 269
            A DK      L+  G   VP  +   G +   DL    +   L+  +  V VKP   GS
Sbjct: 128 VAMDKIMTKRILEVAG---VPQ-VAYTGYIEGEDLEA-AVAETLEKLTFPVFVKPANMGS 187

Query: 270 SIGVSVAYGVVDSLKKANEIISKEIDDKVLVEVFLERGSEFTAIVLDVGSGSVCHPVVLL 329
           S+G+S A      L+ A ++  K  D ++L+E          A  ++VG        +L 
Sbjct: 188 SVGISKAEN-ESELRSAIDLALK-YDSRILIE------QGVVAREIEVG--------ILG 247

Query: 330 PTEVELESHAWADAGE--KD-AIFNYRRKYLPTQQVAYHTPPRFPIDVIESIREGASLLF 389
            T V+       D GE  KD A ++Y+ KY+   ++    P R P++V+  +R  A+  F
Sbjct: 248 NTTVKT-----TDPGEVVKDVAFYDYQAKYI-DNKITMDIPARVPVEVMTQMRAYAAKAF 307

Query: 390 KGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTASGTVVYTDINLISGMEQTSFLFQQAS 449
           + LG C  AR D                 F  T  G +   ++N + G  Q S       
Sbjct: 308 RALGGCGLARCD-----------------FFLTEDGAIYLNELNTMPGFTQWSMYPLLWE 333

Query: 450 KVGFSHSNILRSII 461
            +G S+S++++ ++
Sbjct: 368 NMGLSYSDLIKELV 333

BLAST of Carg12448 vs. ExPASy Swiss-Prot
Match: Q5LYJ2 (D-alanine--D-alanine ligase OS=Streptococcus thermophilus (strain CNRZ 1066) OX=299768 GN=ddl PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 6.2e-24
Identity = 106/374 (28.34%), Postives = 166/374 (44.39%), Query Frame = 0

Query: 90  LICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDPELNAYAISSAQVYSNTPADFDF 149
           L+ GG SAER +S+ SA SV+  +  +  +V  Y+I     +      Q +  TP D D 
Sbjct: 8   LLYGGRSAEREVSVLSAESVMRAVDYNAFEVKTYFI---TQSGDFIKTQEFIETPGD-DE 67

Query: 150 KLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQELLETHNIPFVGTGSTECA 209
           KL +      S       +     +VFPV+HG  GEDG IQ  LET  +P+VGT     +
Sbjct: 68  KLMTNDTVEASQAIKPSDIYEEDAVVFPVLHGPMGEDGSIQGFLETLKLPYVGTNVLSSS 127

Query: 210 HAFDKYNAALELDRLGFITVPNFLVQAGSVSESDLSKWFLRNQLDLSSGKVVVKPVRAGS 269
            A DK      L+  G   V   +   G   E+ +++      L+  +  V VKP   GS
Sbjct: 128 VAMDKIMTKRILEVAGVPQVAYTVYIEGEDLEAAVAE-----TLEKLTFPVFVKPANMGS 187

Query: 270 SIGVSVAYGVVDSLKKANEIISKEIDDKVLVEVFLERGSEFTAIVLDVGSGSVCHPVVLL 329
           S+G+S A      L+ A ++  K  D ++L+E          A  ++VG        +L 
Sbjct: 188 SVGISKAEN-ESELRSAIDLALK-YDSRILIE------QGVVAREIEVG--------ILG 247

Query: 330 PTEVELESHAWADAGE--KD-AIFNYRRKYLPTQQVAYHTPPRFPIDVIESIREGASLLF 389
            T V+       D GE  KD A ++Y+ KY+   ++    P R P++V+  +R  A+  F
Sbjct: 248 NTTVKT-----TDPGEVVKDVAFYDYQAKYI-DNKITMDIPARVPVEVMTQMRAYAAKAF 307

Query: 390 KGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTASGTVVYTDINLISGMEQTSFLFQQAS 449
           + LG C  AR D                 F  T  G +   ++N + G  Q S       
Sbjct: 308 RALGGCGLARCD-----------------FFLTEDGAIYLNELNTMPGFTQWSMYPLLWE 333

Query: 450 KVGFSHSNILRSII 461
            +G S+S+I++ ++
Sbjct: 368 NMGLSYSDIIKELV 333

BLAST of Carg12448 vs. ExPASy Swiss-Prot
Match: Q5M355 (D-alanine--D-alanine ligase OS=Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) OX=264199 GN=ddl PE=3 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.1e-23
Identity = 105/374 (28.07%), Postives = 166/374 (44.39%), Query Frame = 0

Query: 90  LICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDPELNAYAISSAQVYSNTPADFDF 149
           L+ GG SAER +S+ SA SV+  +  +  +V  Y+I     +      Q +  TP D D 
Sbjct: 8   LLYGGRSAEREVSVLSAESVMRAVDYNAFEVKTYFI---TQSGDFIKTQEFIETPGD-DE 67

Query: 150 KLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQELLETHNIPFVGTGSTECA 209
           KL +      S       +     +VFPV+HG  GEDG IQ  LET  +P+VGT     +
Sbjct: 68  KLMTNDTVEASQAIKPSDIYEEDAVVFPVLHGPMGEDGSIQGFLETLKLPYVGTNVLSSS 127

Query: 210 HAFDKYNAALELDRLGFITVPNFLVQAGSVSESDLSKWFLRNQLDLSSGKVVVKPVRAGS 269
            A DK      L+  G   V   +   G   E+ +++      L+  +  V VKP   GS
Sbjct: 128 VAMDKIMTKRILEVAGVPQVAYTVYIEGEDLEAAVAE-----TLEKLTFPVFVKPANMGS 187

Query: 270 SIGVSVAYGVVDSLKKANEIISKEIDDKVLVEVFLERGSEFTAIVLDVGSGSVCHPVVLL 329
           S+G+S A      L+ A ++  K  D ++L+E          A  ++VG        +L 
Sbjct: 188 SVGISKAEN-ESELRSAIDLALK-YDSRILIE------QGVVAREIEVG--------ILG 247

Query: 330 PTEVELESHAWADAGE--KD-AIFNYRRKYLPTQQVAYHTPPRFPIDVIESIREGASLLF 389
            T V+       D GE  KD A ++Y+ KY+   ++    P R P++V+  +R  A+  F
Sbjct: 248 NTTVKT-----TDPGEVVKDVAFYDYQAKYI-DNKITMDIPARVPVEVMTQMRAYAAKAF 307

Query: 390 KGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTASGTVVYTDINLISGMEQTSFLFQQAS 449
           + LG C  AR D                 F  T  G +   ++N + G  Q S       
Sbjct: 308 RALGGCGLARCD-----------------FFLTEDGAIYLNELNTMPGFTQWSMYPLLWE 333

Query: 450 KVGFSHSNILRSII 461
            +G S+S++++ ++
Sbjct: 368 NMGLSYSDLIKELV 333

BLAST of Carg12448 vs. ExPASy TrEMBL
Match: A0A6J1F3B4 (uncharacterized protein LOC111441803 OS=Cucurbita moschata OX=3662 GN=LOC111441803 PE=4 SV=1)

HSP 1 Score: 1844.7 bits (4777), Expect = 0.0e+00
Identity = 958/1081 (88.62%), Postives = 959/1081 (88.71%), Query Frame = 0

Query: 1    MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60
            MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM
Sbjct: 1    MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60

Query: 61   VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120
            VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV
Sbjct: 61   VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120

Query: 121  SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180
            SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH
Sbjct: 121  SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180

Query: 181  GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS 240
            GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS
Sbjct: 181  GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS 240

Query: 241  ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300
            ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV
Sbjct: 241  ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300

Query: 301  EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ 360
            EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ
Sbjct: 301  EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ 360

Query: 361  QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420
            QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA
Sbjct: 361  QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420

Query: 421  SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480
            SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM
Sbjct: 421  SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480

Query: 481  PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540
            PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL
Sbjct: 481  PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540

Query: 541  APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600
            APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR
Sbjct: 541  APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600

Query: 601  ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAGVVSNRQVPIL 660
            ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIA           
Sbjct: 601  ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIA----------- 660

Query: 661  GMCSSSPLYECNYNGIRKLTNIMHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA 720
                                  +HGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA
Sbjct: 661  ----------------------VHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA 720

Query: 721  TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV 780
            TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV
Sbjct: 721  TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV 780

Query: 781  ARLCCADDLAVYVKALEDCLVRIPSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHGMIE 840
            ARLCCADDLAVYVKALEDCLVRIPSNSLSR                        AHGMIE
Sbjct: 781  ARLCCADDLAVYVKALEDCLVRIPSNSLSR------------------------AHGMIE 840

Query: 841  MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP 900
            MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP
Sbjct: 841  MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP 900

Query: 901  SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI 960
            SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI
Sbjct: 901  SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI 960

Query: 961  DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQRNHDAVEDYWDWECPTLVNLVEDHGFISE 1020
            DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQ                             
Sbjct: 961  DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQ----------------------------- 960

Query: 1021 EYYLHWAPERSRASIKRRLFESSMLRAYSVLCSREALAETPPVYPHQFFRRLLDLALERS 1080
                                               ALAETPPVYPHQFFRRLLDLA ERS
Sbjct: 1021 -----------------------------------ALAETPPVYPHQFFRRLLDLAWERS 960

Query: 1081 P 1082
            P
Sbjct: 1081 P 960

BLAST of Carg12448 vs. ExPASy TrEMBL
Match: A0A6J1IXB9 (uncharacterized protein LOC111481502 OS=Cucurbita maxima OX=3661 GN=LOC111481502 PE=4 SV=1)

HSP 1 Score: 1815.4 bits (4701), Expect = 0.0e+00
Identity = 942/1081 (87.14%), Postives = 950/1081 (87.88%), Query Frame = 0

Query: 1    MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60
            MAH ANCSTC VLCKDI+ RASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM
Sbjct: 1    MAHKANCSTCKVLCKDIEPRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60

Query: 61   VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120
            VVKDGVAEMA TEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV
Sbjct: 61   VVKDGVAEMAATEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120

Query: 121  SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180
            SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH
Sbjct: 121  SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180

Query: 181  GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS 240
            GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNA+LELDRLGFITVPNFLVQAGSVS
Sbjct: 181  GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNASLELDRLGFITVPNFLVQAGSVS 240

Query: 241  ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300
            ESDLSKWFLRNQLDLSSGKVVVKP RAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV
Sbjct: 241  ESDLSKWFLRNQLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300

Query: 301  EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ 360
            EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVEL+SH WADAGEKDAIFNYRRKYLPTQ
Sbjct: 301  EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELQSHGWADAGEKDAIFNYRRKYLPTQ 360

Query: 361  QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420
            QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA
Sbjct: 361  QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420

Query: 421  SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480
            SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM
Sbjct: 421  SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480

Query: 481  PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540
            PRRSISSQR+QAYSSTESNQKVF+I GGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL
Sbjct: 481  PRRSISSQRSQAYSSTESNQKVFLIFGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540

Query: 541  APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600
            APSIEQTSSVDLDKNEADMTS+TVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR
Sbjct: 541  APSIEQTSSVDLDKNEADMTSKTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600

Query: 601  ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAGVVSNRQVPIL 660
            ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIA           
Sbjct: 601  ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIA----------- 660

Query: 661  GMCSSSPLYECNYNGIRKLTNIMHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA 720
                                  +HGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA
Sbjct: 661  ----------------------VHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVA 720

Query: 721  TSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV 780
            TSLALNHLSDMGVLTIKKDARRKDELL+IPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV
Sbjct: 721  TSLALNHLSDMGVLTIKKDARRKDELLNIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGV 780

Query: 781  ARLCCADDLAVYVKALEDCLVRIPSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHGMIE 840
            ARLCCADDLAVY KALE CLVRIPSNSLSR                        AHGMIE
Sbjct: 781  ARLCCADDLAVYAKALEYCLVRIPSNSLSR------------------------AHGMIE 840

Query: 841  MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP 900
            MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP
Sbjct: 841  MPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSP 900

Query: 901  SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRI 960
            SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIAN LQLEGFSRI
Sbjct: 901  SVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANALQLEGFSRI 960

Query: 961  DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQRNHDAVEDYWDWECPTLVNLVEDHGFISE 1020
            DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQ                             
Sbjct: 961  DAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQ----------------------------- 960

Query: 1021 EYYLHWAPERSRASIKRRLFESSMLRAYSVLCSREALAETPPVYPHQFFRRLLDLALERS 1080
                                               ALAETPPVYPHQFFRRLLDLALERS
Sbjct: 1021 -----------------------------------ALAETPPVYPHQFFRRLLDLALERS 960

Query: 1081 P 1082
            P
Sbjct: 1081 P 960

BLAST of Carg12448 vs. ExPASy TrEMBL
Match: A0A5D3DMR0 (D-alanine--D-alanine ligase family protein isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00380 PE=4 SV=1)

HSP 1 Score: 1647.9 bits (4266), Expect = 0.0e+00
Identity = 876/1124 (77.94%), Postives = 921/1124 (81.94%), Query Frame = 0

Query: 1    MAHIANC--STCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAA 60
            MA+IANC  STC+ L   +KL  S F PTLSSSFLRLSL  +NH   L SPK ++ R+AA
Sbjct: 1    MAYIANCSYSTCHPLSDVLKLTPSTFAPTLSSSFLRLSLQSNNHTPPLHSPKFQLPRSAA 60

Query: 61   KMVV-KDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDD 120
            K++  KD VAEMA T  EREK S KC ALRVGLICGGPSAERGISLNSARSVLDHIQG D
Sbjct: 61   KVLANKDVVAEMAVTRTEREKQSEKCGALRVGLICGGPSAERGISLNSARSVLDHIQGHD 120

Query: 121  LQVSCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFP 180
            LQVSCYYID ELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFP
Sbjct: 121  LQVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFP 180

Query: 181  VIHGRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAG 240
            VIHGRFGEDGGIQELLETHNIPFVGTGSTE + AFDKY+A+LELDRLGFITVPNFLVQAG
Sbjct: 181  VIHGRFGEDGGIQELLETHNIPFVGTGSTESSRAFDKYDASLELDRLGFITVPNFLVQAG 240

Query: 241  SVSESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDK 300
            SVSE++LSKWF+ NQLDLSSGKVVVKP RAGSSIGVSVAYGVVDSLKKANEIIS+EIDDK
Sbjct: 241  SVSETELSKWFVSNQLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISEEIDDK 300

Query: 301  VLVEVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYL 360
            VLVEVFLE GSEFTAIVLDVGSGSVCHPVVLLPTEVE++S   ADAGEKDAIFNYRRKYL
Sbjct: 301  VLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVEIQSRDVADAGEKDAIFNYRRKYL 360

Query: 361  PTQQVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFG 420
            PTQQVAYHTPPRFPIDVIE+IREGASLLFKGLGLCDFARIDGW+LP+ SH+SS STGKFG
Sbjct: 361  PTQQVAYHTPPRFPIDVIETIREGASLLFKGLGLCDFARIDGWYLPSFSHESSCSTGKFG 420

Query: 421  RTASGTVVYTDINLISGMEQTSFLFQQASK--------------VGFSHSNILRSIIYHA 480
            +T SGTVVYTDINLISGMEQTSFLFQQASK              VGFSHSNILRSIIYHA
Sbjct: 421  KTESGTVVYTDINLISGMEQTSFLFQQASKGFLQLWQKSHVVLLVGFSHSNILRSIIYHA 480

Query: 481  CLRYPSLGSLDCMSGNMPRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNV 540
            CLRYPSLGSLDC+SGN+PRRSISSQ +QAYS+ ES +KVFVI GGDTSERQVSLMSGTNV
Sbjct: 481  CLRYPSLGSLDCISGNVPRRSISSQCSQAYSTHESIRKVFVIFGGDTSERQVSLMSGTNV 540

Query: 541  WLNLQAFDDLEVTPCLLAPSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAA 600
            WLNLQAFD+LEVTPCLLAPSIEQ SSVDLDKNEAD+TSRTVWSLPYSLVLRHTTEEVLAA
Sbjct: 541  WLNLQAFDELEVTPCLLAPSIEQASSVDLDKNEADLTSRTVWSLPYSLVLRHTTEEVLAA 600

Query: 601  CIEAFEPTRAALTSHLRELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVD 660
            CIEA EPTRAALTSHLRELVV DLEEGLKKHSWFAGFDIKDELPV+FSLEQWI+KAKEVD
Sbjct: 601  CIEAIEPTRAALTSHLRELVVNDLEEGLKKHSWFAGFDIKDELPVRFSLEQWIEKAKEVD 660

Query: 661  ATVFIAGVVSNRQVPILG------------MCSSSPL-------YECNYNGIRKL----- 720
            ATVFIAGVVSN QVP L               + SPL          + N +  +     
Sbjct: 661  ATVFIAGVVSNCQVPYLAPSYMLFLTTRFQFSAWSPLLLHMREILTASGNSLMSVPSRIE 720

Query: 721  ---TNIMHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTI 780
                N MHGGIGE+G LQSLLEAKGVPYTGPGVAASNICMDKV+TSLALNHLSD GVLTI
Sbjct: 721  PWNLNNMHGGIGEDGTLQSLLEAKGVPYTGPGVAASNICMDKVSTSLALNHLSDFGVLTI 780

Query: 781  KKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKAL 840
            KKD R+KD+LL  P LN+W DLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKAL
Sbjct: 781  KKDVRKKDDLLRFPILNVWRDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKAL 840

Query: 841  EDCLVRIPSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHGMIEMPKPPPELLIFEPFIE 900
            EDCLVRIPSNSLS+                        AHGMIEMPKPPPELLIFEPFIE
Sbjct: 841  EDCLVRIPSNSLSK------------------------AHGMIEMPKPPPELLIFEPFIE 900

Query: 901  TDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSPSVTVKESGDILSLEEK 960
            TDEIIVSSK  + SERLLWKGQSRWVEITVGVVGTRGSMHSLSPSVTVKESGDILSLEEK
Sbjct: 901  TDEIIVSSKTIDASERLLWKGQSRWVEITVGVVGTRGSMHSLSPSVTVKESGDILSLEEK 960

Query: 961  FQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRIDAFVNVDSGEVLVIEV 1020
            FQGGTGINLTPPPLSIISNESL KCKQHIELIAN LQLEGFSRIDAFV+VDSG+VLVIEV
Sbjct: 961  FQGGTGINLTPPPLSIISNESLGKCKQHIELIANALQLEGFSRIDAFVDVDSGKVLVIEV 1020

Query: 1021 NTVPGMTPSTVLIHQRNHDAVEDYWDWECPTLVNLVEDHGFISEEYYLHWAPERSRASIK 1080
            NTVPGMTPSTVLIHQ                                             
Sbjct: 1021 NTVPGMTPSTVLIHQ--------------------------------------------- 1036

BLAST of Carg12448 vs. ExPASy TrEMBL
Match: A0A5A7TLK1 (D-alanine--D-alanine ligase family protein isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G005710 PE=4 SV=1)

HSP 1 Score: 1638.6 bits (4242), Expect = 0.0e+00
Identity = 861/1083 (79.50%), Postives = 903/1083 (83.38%), Query Frame = 0

Query: 1    MAHIANC--STCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAA 60
            MA+IANC  STC+ L   +KL  S F PTLSSSFLRLSL  +NH   L SPK ++ R+AA
Sbjct: 1    MAYIANCSYSTCHPLSDVLKLTPSTFAPTLSSSFLRLSLQSNNHTPPLHSPKFQLPRSAA 60

Query: 61   KMVV-KDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDD 120
            K++  KD VAEMA T  EREK S KC ALRVGLICGGPSAERGISLNSARSVLDHIQG D
Sbjct: 61   KVLANKDVVAEMAVTRTEREKQSEKCGALRVGLICGGPSAERGISLNSARSVLDHIQGHD 120

Query: 121  LQVSCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFP 180
            LQVSCYYID ELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFP
Sbjct: 121  LQVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFP 180

Query: 181  VIHGRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAG 240
            VIHGRFGEDGGIQELLETHNIPFVGTGSTE + AFDKY+A+LELDRLGFITVPNFLVQAG
Sbjct: 181  VIHGRFGEDGGIQELLETHNIPFVGTGSTESSRAFDKYDASLELDRLGFITVPNFLVQAG 240

Query: 241  SVSESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDK 300
            SVSE++LSKWF+ NQLDLSSGKVVVKP RAGSSIGVSVAYGVVDSLKKANEIIS+EIDDK
Sbjct: 241  SVSETELSKWFVSNQLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISEEIDDK 300

Query: 301  VLVEVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYL 360
            VLVEVFLE GSEFTAIVLDVGSGSVCHPVVLLPTEVE++S   ADAGEKDAIFNYRRKYL
Sbjct: 301  VLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVEIQSRDVADAGEKDAIFNYRRKYL 360

Query: 361  PTQQVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFG 420
            PTQQVAYHTPPRFPIDVIE+IREGASLLFKGLGLCDFARIDGW+LP+ SH+SS STGKFG
Sbjct: 361  PTQQVAYHTPPRFPIDVIETIREGASLLFKGLGLCDFARIDGWYLPSFSHESSCSTGKFG 420

Query: 421  RTASGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMS 480
            +T SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRY SLGSLDC+S
Sbjct: 421  KTESGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYSSLGSLDCIS 480

Query: 481  GNMPRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTP 540
            GN+PRRSISSQR+QAYS+ ES +KVFVI GGDTSERQVSLMSGTNVWLNLQAFD+LEVTP
Sbjct: 481  GNVPRRSISSQRSQAYSTHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQAFDELEVTP 540

Query: 541  CLLAPSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTS 600
            CLLAPSIEQ SSVDLDKNEAD+TSRTVWSLPYSLVLRHTTEEVLAACIEA EPTRAALTS
Sbjct: 541  CLLAPSIEQASSVDLDKNEADLTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTS 600

Query: 601  HLRELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAGVVSNRQV 660
            HLRELVVTDLEEGLKKHSWFAGFDIKDELPV+FSLEQWI+KAKEVDATVFIA        
Sbjct: 601  HLRELVVTDLEEGLKKHSWFAGFDIKDELPVRFSLEQWIEKAKEVDATVFIA-------- 660

Query: 661  PILGMCSSSPLYECNYNGIRKLTNIMHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMD 720
                                     +HGGIGE+G LQSLLEAKGVPYTGPGVAASNICMD
Sbjct: 661  -------------------------VHGGIGEDGTLQSLLEAKGVPYTGPGVAASNICMD 720

Query: 721  KVATSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCS 780
            KV+TSLALNHLSD GVLT KKD R+KD+LL  P LN+W DLTCKLQCQSLCVKPARDGCS
Sbjct: 721  KVSTSLALNHLSDFGVLTTKKDVRKKDDLLRFPILNVWRDLTCKLQCQSLCVKPARDGCS 780

Query: 781  TGVARLCCADDLAVYVKALEDCLVRIPSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHG 840
            TGVARLCCADDLAVYVKALEDCLVRIPSNSLS+                        AHG
Sbjct: 781  TGVARLCCADDLAVYVKALEDCLVRIPSNSLSK------------------------AHG 840

Query: 841  MIEMPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHS 900
            MIEMPKPPPELLIFEPFIETDEIIVSSK  + SERLLWKGQSRWVEITVGVVGTRGSMHS
Sbjct: 841  MIEMPKPPPELLIFEPFIETDEIIVSSKTIDASERLLWKGQSRWVEITVGVVGTRGSMHS 900

Query: 901  LSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGF 960
            LSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESL KCKQHIELIAN LQLEGF
Sbjct: 901  LSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLGKCKQHIELIANALQLEGF 960

Query: 961  SRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQRNHDAVEDYWDWECPTLVNLVEDHGF 1020
            SRIDAFV+VDSG+VLVIEVNTVPGMTPSTVLIHQ                          
Sbjct: 961  SRIDAFVDVDSGKVLVIEVNTVPGMTPSTVLIHQ-------------------------- 962

Query: 1021 ISEEYYLHWAPERSRASIKRRLFESSMLRAYSVLCSREALAETPPVYPHQFFRRLLDLAL 1080
                                                  ALAETPPVYPHQFFRRLLDLAL
Sbjct: 1021 --------------------------------------ALAETPPVYPHQFFRRLLDLAL 962

BLAST of Carg12448 vs. ExPASy TrEMBL
Match: A0A1S3B5H4 (uncharacterized protein LOC103486245 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486245 PE=4 SV=1)

HSP 1 Score: 1638.6 bits (4242), Expect = 0.0e+00
Identity = 861/1083 (79.50%), Postives = 903/1083 (83.38%), Query Frame = 0

Query: 1    MAHIANC--STCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAA 60
            MA+IANC  STC+ L   +KL  S F PTLSSSFLRLSL  +NH   L SPK ++ R+AA
Sbjct: 1    MAYIANCSYSTCHPLSDVLKLTPSTFAPTLSSSFLRLSLQSNNHTPPLHSPKFQLPRSAA 60

Query: 61   KMVV-KDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDD 120
            K++  KD VAEMA T  EREK S KC ALRVGLICGGPSAERGISLNSARSVLDHIQG D
Sbjct: 61   KVLANKDVVAEMAVTRTEREKQSEKCGALRVGLICGGPSAERGISLNSARSVLDHIQGHD 120

Query: 121  LQVSCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFP 180
            LQVSCYYID ELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFP
Sbjct: 121  LQVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFP 180

Query: 181  VIHGRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAG 240
            VIHGRFGEDGGIQELLETHNIPFVGTGSTE + AFDKY+A+LELDRLGFITVPNFLVQAG
Sbjct: 181  VIHGRFGEDGGIQELLETHNIPFVGTGSTESSRAFDKYDASLELDRLGFITVPNFLVQAG 240

Query: 241  SVSESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDK 300
            SVSE++LSKWF+ NQLDLSSGKVVVKP RAGSSIGVSVAYGVVDSLKKANEIIS+EIDDK
Sbjct: 241  SVSETELSKWFVSNQLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISEEIDDK 300

Query: 301  VLVEVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYL 360
            VLVEVFLE GSEFTAIVLDVGSGSVCHPVVLLPTEVE++S   ADAGEKDAIFNYRRKYL
Sbjct: 301  VLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVEIQSRDVADAGEKDAIFNYRRKYL 360

Query: 361  PTQQVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFG 420
            PTQQVAYHTPPRFPIDVIE+IREGASLLFKGLGLCDFARIDGW+LP+ SH+SS STGKFG
Sbjct: 361  PTQQVAYHTPPRFPIDVIETIREGASLLFKGLGLCDFARIDGWYLPSFSHESSCSTGKFG 420

Query: 421  RTASGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMS 480
            +T SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDC+S
Sbjct: 421  KTESGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCIS 480

Query: 481  GNMPRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTP 540
            GN+PRRSISSQ +QAYS+ ES +KVFVI GGDTSERQVSLMSGTNVWLNLQAFD+LEVTP
Sbjct: 481  GNVPRRSISSQCSQAYSTHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQAFDELEVTP 540

Query: 541  CLLAPSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTS 600
            CLLAPSIEQ SSVDLDKNEAD+TSRTVWSLPYSLVLRHTTEEVLAACIEA EPTRAALTS
Sbjct: 541  CLLAPSIEQASSVDLDKNEADLTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTS 600

Query: 601  HLRELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAGVVSNRQV 660
            HLRELVV DLEEGLKKHSWFAGFDIKDELPV+FSLEQWI+KAKEVDATVFIA        
Sbjct: 601  HLRELVVNDLEEGLKKHSWFAGFDIKDELPVRFSLEQWIEKAKEVDATVFIA-------- 660

Query: 661  PILGMCSSSPLYECNYNGIRKLTNIMHGGIGENGMLQSLLEAKGVPYTGPGVAASNICMD 720
                                     +HGGIGE+G LQSLLEAKGVPYTGPGVAASNICMD
Sbjct: 661  -------------------------VHGGIGEDGTLQSLLEAKGVPYTGPGVAASNICMD 720

Query: 721  KVATSLALNHLSDMGVLTIKKDARRKDELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCS 780
            KV+TSLALNHLSD GVLTIKKD R+KD+LL  P LN+W DLTCKLQCQSLCVKPARDGCS
Sbjct: 721  KVSTSLALNHLSDFGVLTIKKDVRKKDDLLRFPILNVWRDLTCKLQCQSLCVKPARDGCS 780

Query: 781  TGVARLCCADDLAVYVKALEDCLVRIPSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHG 840
            TGVARLCCADDLAVYVKALEDCLVRIPSNSLS+                        AHG
Sbjct: 781  TGVARLCCADDLAVYVKALEDCLVRIPSNSLSK------------------------AHG 840

Query: 841  MIEMPKPPPELLIFEPFIETDEIIVSSKYTNESERLLWKGQSRWVEITVGVVGTRGSMHS 900
            MIEMPKPPPELLIFEPFIETDEIIVSSK  + SERLLWKGQSRWVEITVGVVGTRGSMHS
Sbjct: 841  MIEMPKPPPELLIFEPFIETDEIIVSSKTIDASERLLWKGQSRWVEITVGVVGTRGSMHS 900

Query: 901  LSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEGF 960
            LSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESL KCKQHIELIAN LQLEGF
Sbjct: 901  LSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLGKCKQHIELIANALQLEGF 960

Query: 961  SRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQRNHDAVEDYWDWECPTLVNLVEDHGF 1020
            SRIDAFV+VDSG+VLVIEVNTVPGMTPSTVLIHQ                          
Sbjct: 961  SRIDAFVDVDSGKVLVIEVNTVPGMTPSTVLIHQ-------------------------- 962

Query: 1021 ISEEYYLHWAPERSRASIKRRLFESSMLRAYSVLCSREALAETPPVYPHQFFRRLLDLAL 1080
                                                  ALAETPPVYPHQFFRRLLDLAL
Sbjct: 1021 --------------------------------------ALAETPPVYPHQFFRRLLDLAL 962

BLAST of Carg12448 vs. TAIR 10
Match: AT3G08840.2 (D-alanine--D-alanine ligase family )

HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 604/996 (60.64%), Postives = 713/996 (71.59%), Query Frame = 0

Query: 84   EALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDPELNAYAISSAQVYSNT 143
            ++LRVGLICGGPSAERGISLNSARSVLDHIQGD + VSCYYIDP+L A+AISSAQVYSNT
Sbjct: 76   KSLRVGLICGGPSAERGISLNSARSVLDHIQGDGINVSCYYIDPDLKAFAISSAQVYSNT 135

Query: 144  PADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQELLETHNIPFVGT 203
            P+DFDFKL+SLA+GF SL++ AEHL ++VDIVFPVIHGRFGEDGGIQELLE+HNIPFVGT
Sbjct: 136  PSDFDFKLESLAQGFSSLSELAEHLVSAVDIVFPVIHGRFGEDGGIQELLESHNIPFVGT 195

Query: 204  GSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVSESDLSKWFLRNQLDLSSGKVVVK 263
            GS EC  AFDKY A+LEL  LGF+TVPN+LVQ   V +S+++ WF  NQLDL  GKVVVK
Sbjct: 196  GSRECFRAFDKYEASLELKELGFMTVPNYLVQGTGVDKSEIALWFTDNQLDLEMGKVVVK 255

Query: 264  PVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLVEVFLERGSEFTAIVLDVGSGSVC 323
            P +AGSSIGV VA+GV DS+KKA E+I + IDD+V+VEVF+E   EFTAIVLDVGSGSVC
Sbjct: 256  PAKAGSSIGVKVAFGVNDSIKKATELILEGIDDRVVVEVFIEDAYEFTAIVLDVGSGSVC 315

Query: 324  HPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQQVAYHTPPRFPIDVIESIREGAS 383
            HPVVL+PTEV+L+ H   D  E +AIF+YRRKYLPTQQV YHTPPRFPI VI+SIRE AS
Sbjct: 316  HPVVLMPTEVQLQFHGIGDPKE-NAIFDYRRKYLPTQQVTYHTPPRFPIHVIKSIREEAS 375

Query: 384  LLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTASGTVVYTDINLISGMEQTSFLFQ 443
            L+F+ LGL DFARIDGW+L  +S+ SS  +   G T SG +++TDINLISGMEQTSFLFQ
Sbjct: 376  LIFQKLGLRDFARIDGWYLAPNSNLSSPVSETLGGTKSGDIIFTDINLISGMEQTSFLFQ 435

Query: 444  QASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNMPRRSISSQRTQAYSSTESNQKVF 503
            QASKVGFSHSNILR+I++ AC R+P L   +     +       Q +     +E  QKVF
Sbjct: 436  QASKVGFSHSNILRTIVHRACSRFPHLDWYNYGYSQL------LQGSTTLEVSEDPQKVF 495

Query: 504  VILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLLAPSIEQTSSVDLDKNEADMTSRT 563
            VI GGDTSERQVS+MSGTNVW+NLQ + DL VTPCLL+PS+  +         +++ +R 
Sbjct: 496  VIFGGDTSERQVSVMSGTNVWVNLQRYVDLNVTPCLLSPSLSNSLGA-----SSNLDNRE 555

Query: 564  VWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLRELVVTDLEEGLKKHSWFAGFDIK 623
            VW LPYS+VLRHT EEVLAAC+EA EP RA  TS L++ V+ DL +G K  SWFAGFDI 
Sbjct: 556  VWVLPYSVVLRHTAEEVLAACLEAVEPVRALFTSLLQKQVMEDLMDGFKNQSWFAGFDIT 615

Query: 624  DELPVKFSLEQWIKKAKEVDATVFIAGVVSNRQVPILGMCSSSPLYECNYNGIRKLTNIM 683
            DELP K+SL++WIK AKE  ATVFIA                                 +
Sbjct: 616  DELPRKYSLKEWIKHAKEAQATVFIA---------------------------------V 675

Query: 684  HGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDARRK 743
            HGGIGE+G LQ LLE +GV YTGPGV AS  CMDKV TS AL++LS+ G+ TI KD +R 
Sbjct: 676  HGGIGEDGTLQGLLEDEGVSYTGPGVLASRTCMDKVMTSQALSNLSEFGIHTISKDVKRT 735

Query: 744  DELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRI 803
            ++++H    N+W +L  KLQC +LCVKPA+DGCSTGVARLC ++DLAVYV+AL+DC+ RI
Sbjct: 736  EDIMHETFPNVWDELIKKLQCLTLCVKPAKDGCSTGVARLCSSEDLAVYVQALKDCIPRI 795

Query: 804  PSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHGMIEMPKPPPELLIFEPFIETDEIIVS 863
            P N+LS+                         HGMIEMP P PE LIFEPF+ETDEIIVS
Sbjct: 796  PPNTLSK------------------------THGMIEMPNPTPEFLIFEPFVETDEIIVS 855

Query: 864  SKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSPSVTVKESGDILSLEEKFQGGTGI 923
            SK     ++L WKG+ RWVE+TVGV+G RGSMHSLSPS+TVKESGDILSLEEKFQGGTGI
Sbjct: 856  SK---AKQQLSWKGRRRWVEMTVGVIGKRGSMHSLSPSLTVKESGDILSLEEKFQGGTGI 915

Query: 924  NLTPPPLSIISNESLEKCKQHIELIANTLQLEGFSRIDAFVNVDSGEVLVIEVNTVPGMT 983
            NLTPPP +I+S E+LE+CKQ IELIA TL LEGFSRIDAFV+V++GEVLVIEVNTVPGMT
Sbjct: 916  NLTPPPPTIMSKEALERCKQGIELIAETLGLEGFSRIDAFVHVETGEVLVIEVNTVPGMT 935

Query: 984  PSTVLIHQRNHDAVEDYWDWECPTLVNLVEDHGFISEEYYLHWAPERSRASIKRRLFESS 1043
            PSTVLI Q                                                    
Sbjct: 976  PSTVLIQQ---------------------------------------------------- 935

Query: 1044 MLRAYSVLCSREALAETPPVYPHQFFRRLLDLALER 1080
                        ALAE PP+YP QFFR LL LA +R
Sbjct: 1036 ------------ALAEQPPMYPPQFFRTLLHLATQR 935

BLAST of Carg12448 vs. TAIR 10
Match: AT3G08840.3 (D-alanine--D-alanine ligase family )

HSP 1 Score: 1018.8 bits (2633), Expect = 3.1e-297
Identity = 527/835 (63.11%), Postives = 627/835 (75.09%), Query Frame = 0

Query: 84  EALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDPELNAYAISSAQVYSNT 143
           ++LRVGLICGGPSAERGISLNSARSVLDHIQGD + VSCYYIDP+L A+AISSAQVYSNT
Sbjct: 76  KSLRVGLICGGPSAERGISLNSARSVLDHIQGDGINVSCYYIDPDLKAFAISSAQVYSNT 135

Query: 144 PADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQELLETHNIPFVGT 203
           P+DFDFKL+SLA+GF SL++ AEHL ++VDIVFPVIHGRFGEDGGIQELLE+HNIPFVGT
Sbjct: 136 PSDFDFKLESLAQGFSSLSELAEHLVSAVDIVFPVIHGRFGEDGGIQELLESHNIPFVGT 195

Query: 204 GSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVSESDLSKWFLRNQLDLSSGKVVVK 263
           GS EC  AFDKY A+LEL  LGF+TVPN+LVQ   V +S+++ WF  NQLDL  GKVVVK
Sbjct: 196 GSRECFRAFDKYEASLELKELGFMTVPNYLVQGTGVDKSEIALWFTDNQLDLEMGKVVVK 255

Query: 264 PVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLVEVFLERGSEFTAIVLDVGSGSVC 323
           P +AGSSIGV VA+GV DS+KKA E+I + IDD+V+VEVF+E   EFTAIVLDVGSGSVC
Sbjct: 256 PAKAGSSIGVKVAFGVNDSIKKATELILEGIDDRVVVEVFIEDAYEFTAIVLDVGSGSVC 315

Query: 324 HPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQQVAYHTPPRFPIDVIESIREGAS 383
           HPVVL+PTEV+L+ H   D  E +AIF+YRRKYLPTQQV YHTPPRFPI VI+SIRE AS
Sbjct: 316 HPVVLMPTEVQLQFHGIGDPKE-NAIFDYRRKYLPTQQVTYHTPPRFPIHVIKSIREEAS 375

Query: 384 LLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTASGTVVYTDINLISGMEQTSFLFQ 443
           L+F+ LGL DFARIDGW+L  +S+ SS  +   G T SG +++TDINLISGMEQTSFLFQ
Sbjct: 376 LIFQKLGLRDFARIDGWYLAPNSNLSSPVSETLGGTKSGDIIFTDINLISGMEQTSFLFQ 435

Query: 444 QASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNMPRRSISSQRTQAYSSTESNQKVF 503
           QASKVGFSHSNILR+I++ AC R+P L   +     +       Q +     +E  QKVF
Sbjct: 436 QASKVGFSHSNILRTIVHRACSRFPHLDWYNYGYSQL------LQGSTTLEVSEDPQKVF 495

Query: 504 VILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLLAPSIEQTSSVDLDKNEADMTSRT 563
           VI GGDTSERQVS+MSGTNVW+NLQ + DL VTPCLL+PS+  +         +++ +R 
Sbjct: 496 VIFGGDTSERQVSVMSGTNVWVNLQRYVDLNVTPCLLSPSLSNSLGA-----SSNLDNRE 555

Query: 564 VWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLRELVVTDLEEGLKKHSWFAGFDIK 623
           VW LPYS+VLRHT EEVLAAC+EA EP RA  TS L++ V+ DL +G K  SWFAGFDI 
Sbjct: 556 VWVLPYSVVLRHTAEEVLAACLEAVEPVRALFTSLLQKQVMEDLMDGFKNQSWFAGFDIT 615

Query: 624 DELPVKFSLEQWIKKAKEVDATVFIAGVVSNRQVPILGMCSSSPLYECNYNGIRKLTNIM 683
           DELP K+SL++WIK AKE  ATVFIA                                 +
Sbjct: 616 DELPRKYSLKEWIKHAKEAQATVFIA---------------------------------V 675

Query: 684 HGGIGENGMLQSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDARRK 743
           HGGIGE+G LQ LLE +GV YTGPGV AS  CMDKV TS AL++LS+ G+ TI KD +R 
Sbjct: 676 HGGIGEDGTLQGLLEDEGVSYTGPGVLASRTCMDKVMTSQALSNLSEFGIHTISKDVKRT 735

Query: 744 DELLHIPTLNLWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRI 803
           ++++H    N+W +L  KLQC +LCVKPA+DGCSTGVARLC ++DLAVYV+AL+DC+ RI
Sbjct: 736 EDIMHETFPNVWDELIKKLQCLTLCVKPAKDGCSTGVARLCSSEDLAVYVQALKDCIPRI 795

Query: 804 PSNSLSRVEILMCISRNFSSCDAIRIHKDEPAHGMIEMPKPPPELLIFEPFIETDEIIVS 863
           P N+LS+                         HGMIEMP P PE LIFEPF+ETDEIIVS
Sbjct: 796 PPNTLSK------------------------THGMIEMPNPTPEFLIFEPFVETDEIIVS 838

Query: 864 SKYTNESERLLWKGQSRWVEITVGVVGTRGSMHSLSPSVTVKESGDILSLEEKFQ 919
           SK     ++L WKG+ RWVE+TVGV+G RGSMHSLSPS+TVKESGDILSLEEKFQ
Sbjct: 856 SK---AKQQLSWKGRRRWVEMTVGVIGKRGSMHSLSPSLTVKESGDILSLEEKFQ 838

BLAST of Carg12448 vs. TAIR 10
Match: AT3G08840.1 (D-alanine--D-alanine ligase family )

HSP 1 Score: 619.4 bits (1596), Expect = 5.5e-177
Identity = 313/450 (69.56%), Postives = 368/450 (81.78%), Query Frame = 0

Query: 84  EALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDPELNAYAISSAQVYSNT 143
           ++LRVGLICGGPSAERGISLNSARSVLDHIQGD + VSCYYIDP+L A+AISSAQVYSNT
Sbjct: 76  KSLRVGLICGGPSAERGISLNSARSVLDHIQGDGINVSCYYIDPDLKAFAISSAQVYSNT 135

Query: 144 PADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIHGRFGEDGGIQELLETHNIPFVGT 203
           P+DFDFKL+SLA+GF SL++ AEHL ++VDIVFPVIHGRFGEDGGIQELLE+HNIPFVGT
Sbjct: 136 PSDFDFKLESLAQGFSSLSELAEHLVSAVDIVFPVIHGRFGEDGGIQELLESHNIPFVGT 195

Query: 204 GSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVSESDLSKWFLRNQLDLSSGKVVVK 263
           GS EC  AFDKY A+LEL  LGF+TVPN+LVQ   V +S+++ WF  NQLDL  GKVVVK
Sbjct: 196 GSRECFRAFDKYEASLELKELGFMTVPNYLVQGTGVDKSEIALWFTDNQLDLEMGKVVVK 255

Query: 264 PVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLVEVFLERGSEFTAIVLDVGSGSVC 323
           P +AGSSIGV VA+GV DS+KKA E+I + IDD+V+VEVF+E   EFTAIVLDVGSGSVC
Sbjct: 256 PAKAGSSIGVKVAFGVNDSIKKATELILEGIDDRVVVEVFIEDAYEFTAIVLDVGSGSVC 315

Query: 324 HPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQQVAYHTPPRFPIDVIESIREGAS 383
           HPVVL+PTEV+L+ H   D  E +AIF+YRRKYLPTQQV YHTPPRFPI VI+SIRE AS
Sbjct: 316 HPVVLMPTEVQLQFHGIGDPKE-NAIFDYRRKYLPTQQVTYHTPPRFPIHVIKSIREEAS 375

Query: 384 LLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTASGTVVYTDINLISGMEQTSFLFQ 443
           L+F+ LGL DFARIDGW+L  +S+ SS  +   G T SG +++TDINLISGMEQTSFLFQ
Sbjct: 376 LIFQKLGLRDFARIDGWYLAPNSNLSSPVSETLGGTKSGDIIFTDINLISGMEQTSFLFQ 435

Query: 444 QASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNMPRRSISSQRTQAYSSTESNQKVF 503
           QASKVGFSHSNILR+I++ AC R+P L   +     +       Q +     +E  QKVF
Sbjct: 436 QASKVGFSHSNILRTIVHRACSRFPHLDWYNYGYSQL------LQGSTTLEVSEDPQKVF 495

Query: 504 VILGGDTSERQVSLMSGTNVWLNLQAFDDL 534
           VI GGDTSERQVS+MSGTNVW+NLQ + D+
Sbjct: 496 VIFGGDTSERQVSVMSGTNVWVNLQRYVDV 518