Carg12430 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12430
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPhosphoinositide phospholipase C
LocationCarg_Chr01: 3551644 .. 3556135 (+)
RNA-Seq ExpressionCarg12430
SyntenyCarg12430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTGAAATCAACCCATTTCCCTTTTTTGCTTCTCTCTCTCTCTCTCCCTCCATGGGAAATTACAGGGTTTGTCTCTGTTTCATCAGAAGGTTTAAGATGTCCACAGACGAGCCACCGTCGGACGTCAAGGAGGCGTTTCTCAAATACGCCAGCGCCGCCGCTGCCAACATGACCGTCGAGCAGTTCCACTCGTTTCTTGTGGAGTTTCAAGGAGATCAGACAGCGTCAATGGCCGATGCTCAACGGATTGTTGAGCAAGTGCTGCAGCGGCGGCATCATGTGATCTCCAAGCTCACAAAACACAGTCTCAATCTTGATGATTTCTATTACTATCTCTTCTCTATGGATCTCAATCCCCCAGTTTCAGATCAGGTGAGATATCAATTTCTCTTATTTCTCCTGTTTCCTTGAAATTTGGAAATTTTGGTTTCTGGGTCTTGTACCTCAACCTAAAACCCCAGATTCTTAGATATACAAGGGTTTCATCATTTACAGGTTCATCAGGATATGAGAGCTCCACTGTCCCATTATTACATTTATACTAGCCACAATACATACTTAACTGGGAATCAATTGAGCAGTGATTCCAGTGATGTTCCGATCATAAATGCACTGAAAAGTGGTGTGAGAGTCGTGGAGCTTGATTTGTGGCCAAACTCTGAAGAAGATGACATAGATGTTCTTCATGGAAGGTGAGAGAACTAAGAACTAAGAAATGGAATTGTTGGTTTTGACTGAAATATAGGAAGAATGTAGTTTGTCTGAAGTTGAATGTGTAATCTTTTCTTTAGGACCTTGACAACACCTGTGGAATTCGTTAGATGTCTGAAATCTATTAAAGAGAATGCTTTCATAGCATCTCCATATCCTGTCATAATTACTCTAGAAGACCACCTAACTCCTGATCTTCAAGCTAAAGCAGCCGAGGTATCTTTTTCCCCTCTCAAATTGTTTACAGAAAGAGTAGTTTTGTTCATACTTCGAAGATTTATGGGAGGGAGATGCTTGTTTTTGTTTGCAGAACATCAAGGAAACATTTGGAGACATGCTGTTCTTTCCTGAAGCAGAATATCTACAAGAATTTCCTTCTCCAGAAGAATTGAAGTATCGGATCATCATTTCAACAAAACCCCCTAAAGAATATCTCAGATCCAAAAATGTGAAAGGGGAGAATTCTTCCAGTGAAGATGAAACGGATGATGAAGATGGGGTCGATACGGTCTGCTTTCCGTTGAAATTTACCACATTTTAGCTGTGTTTTTCTAGTCATTTTCTGGTTTCCAGTATCTTGATGAAGTATACAACTCTCTATTGGGTTGCAGAGTGAGAGTGAATCCAGCGAGAGTGACGACAGTGATTACGAATCTTACGCTACAGGTGTATCCGAATACAAACGCCTAATTGCCATTCATGCTGGCAAACCAAAGGGGGGATTGAAGGAAGCTCTAAAAGTTGGAGCTGATAAAGCTAGACGCCTTAGCTTGAGTGAAAAGGCACTAAAAAAGGCAACTAAATCTCATGGAATGGATGTCATTAGGTAATAGTAACATACATGTCGTCAGTTTACTCCAGAAAGAAATTAAGAGAAGTTTAAAGAATTCATTTCCCAAACTGACACCAATCTTGCTCTGTTCTTGCTTTCAGATTCACCCAGAAGAACATGTTAAGGATATATCCGAAGGGAACTCGAGTAAACTCTTCCAACTACAACCCAATGAGTGCTTGGTTACACGGGGCTCAGATGGTTGCTTTCAATATGCAGGTATTTTCATTTTCTCTAGGATCAAATAACATCACTGTCCTTGTTCTTCTAAAAAAATAAATTTAATTTGACCATCACAGGGATACGGAAAGTTTCTCAGTTTAATGTACGGGATGTTTCGATCGAACGGAGGTTGTGGTTATGTGAAAAAACCTGATTTTCTACTGACAGTTGATCCGCATGGCAAGGTGTTTGATCCTAATGCAAAATTGCCAGTTAAAAAAACTTTGAAGGTAGGCATTTTGCCAAACTAGATCATGTATCAAGTCCGGCTGGACTTGCTCTCTAAAACATTATATTGCCAATGACAGGTGAAAGTCTACATGGGTGTTGGCTGGAATTTGGATTTTGAAAGCACCCACTTTCATTTGTACTCCCCTCCTGACTTCTACGCACGGGTAAGTTTCAAATGAATGGAAATCTAGTTAACCCAAGTCCATAGTTCAAGCAATATTGTTACCTTCACCGACTGCATATTGACCATGTTAGCCTTATGCAGATTGGAATCGCAGGAGCTCCAGATGATGCGATAATAAAGAGAACAGAGATCAAAGAGGAGACTTGGAGCCCAGTTTGGAACGAAGAGTTTACATATCCTTTGACCATTCCTGAACTGGCTTTACTTCGAATCGAAGTGTATAGTTATAACATTTCAGATAAGGATGTTTTTGGAGGACAAAACTGTCTGCCTGTTCCTGAAGTGAAGGCAGGCATCCATGCTGTTCCTCTTTTTGACCGTAAAGGATCAAAGTACAGTTCTGTAAAGCTTCTATTGCGTTTTGAGTTTTCCTGAATACAATTTCTTATCCTTTGGGGCAACATCTCAGTGGAGTGAATTTTTCCTGTCAACTGTTGTCTATAGTGGAGATTATTGCTGTTCTTTGACTAGAGAGAGTTGGATTCTTACATTTTTCATAGGGTAGAGAAAAGGCATCGAATATGGGGTCGCCACTTTTCTCTCTTTCCTTTTTCTCTTTTCCTTCTTTGATGCCATTGTACTATAAAGCAGATTCCCTCTGTTCAAGGAGAGTGATTAATTCTGTAAAGAAAAACAGAGACAGGAGAATAAATGGATACATGAACAAAATCGTGTATTTTATTGATACCTTGTCTTCCTAATGGATGCAGTTGATCGAATCACTATACTGAATAGCTGAGGTAACAGTTTGCTGTAAAGCAGCAGACTCTTTAGGATTTAGATGGCTCTATCAGACATCCTAAATTCTAGCAACAGCCTATTCCTGCAATGCAAACTAGGCTGGTCAAATTTGGAATCACAAGCAACTACTTGCCAGTTCCACCACATTTGCTCTCTCTAAGAAAAGAACATCTTGCCATGGACACAGATTCCAACTTCGGCTGGAAGTCATTCTTTAGGGATTGAAATGGAAATTCCCCCTCCCCCTCTTTTTCACTCATTCTTCCACAGATCCAAGTAGGACAAAGGATAAAAAAAAGAAAACACCACGCGGAAAGTGCAGGTTTGGTGTGTCTGCTCTGTATCATTGTAAAGAATGAGTAAGCACGGTTTCAGGGTCTGTTTCTTTTTCAGTAGAAGATTCAGGACAAATGCAGCAGAAGCACCAGAAGATGTGAAGATGATGTTCAATAAATACTCAAAACATGGCACAATGAACATTGATCAATTGCGGACGTTTTTGGAAGAGATTCAAGGAGAAACATCTGAGACAAAAGCTGAGGACATCTTCAACAGTCTCAAGCACCTCAACATCTTTCAGAGAAGAGGCCTTTATCTCGAAGATTTCTTCCGATATCTTCTTGGAGACCTTAATCTTGCTTTCCCGCACTGCCAAGGGGTTATCTCTCTTTCTTCTCTTTACTTTTCAAGCCAATCTACAATATATGCAATGTCACACCATCCATAAAACCAGAGCATCATTCAGAGATAAAAATTCATTAAAATGTATTTTGATATTCTGTTTTGTCATAGACATTACAATCTATTTTTCTAGAACAAAGATTTTCTTCTCAATTCCAAAATTATATGTTTATGAACAGTGAGTTTAATATGAATAATAATATTAAACTTTACAAGTTTTATAATACACTAATGTATTTAAACTCTGGTTCTTACTATTGTCTCATATTCTTCACAATTTTGGAGTATAGTGATCCAAATCTGATTTCCTACTTGCATATCGACTATATTATGACATAGTACTAAAAGTTAATTTAAGTCCTTGTCATTAAGTCTATCTATAATGTGTTTATTAGAAATCTTATCTGGCATCACCTAAACTAATGTTTTGAAAAAGATGTATCAAGTTTTTTCTTAAATCCAATATAAACAATTTAATAATCACGGAAGTTTAAGGATCAAAATAAAATATATTTTTTTAGCAGGAGAGATAAAACAAGTCTGAATGTTTAAGGACAAAGATGTAAGTTAACAAAAGAAGTATAACTTCCACTTCTACCAATAGTACTTTCCTTCAATTTTCACTGAAAGTTCTAAAATAAATTTGCAAAATAAGCCATGATATTATTGATTAATAGTTGGGAACTGGGTGCAGGTTTATCAGGATATGGGAGCCCCATTGTCTCACTACTACATATTTACAGGCCACAATTCCTACTTAACAGGGAATCAACTGTGTAGTAAAAGCAGTGTGACTCCTATCATAAGGGCTCTAAAAAGAGGAGTAAGAGCAATTGAGTTGGACTTGTGGCCTACATCAAAGATGAATGGCATTGAGGTTCGTCATGGA

mRNA sequence

AATTGAAATCAACCCATTTCCCTTTTTTGCTTCTCTCTCTCTCTCTCCCTCCATGGGAAATTACAGGGTTTGTCTCTGTTTCATCAGAAGGTTTAAGATGTCCACAGACGAGCCACCGTCGGACGTCAAGGAGGCGTTTCTCAAATACGCCAGCGCCGCCGCTGCCAACATGACCGTCGAGCAGTTCCACTCGTTTCTTGTGGAGTTTCAAGGAGATCAGACAGCGTCAATGGCCGATGCTCAACGGATTGTTGAGCAAGTGCTGCAGCGGCGGCATCATGTGATCTCCAAGCTCACAAAACACAGTCTCAATCTTGATGATTTCTATTACTATCTCTTCTCTATGGATCTCAATCCCCCAGTTTCAGATCAGGTTCATCAGGATATGAGAGCTCCACTGTCCCATTATTACATTTATACTAGCCACAATACATACTTAACTGGGAATCAATTGAGCAGTGATTCCAGTGATGTTCCGATCATAAATGCACTGAAAAGTGGTGTGAGAGTCGTGGAGCTTGATTTGTGGCCAAACTCTGAAGAAGATGACATAGATGTTCTTCATGGAAGGACCTTGACAACACCTGTGGAATTCGTTAGATGTCTGAAATCTATTAAAGAGAATGCTTTCATAGCATCTCCATATCCTGTCATAATTACTCTAGAAGACCACCTAACTCCTGATCTTCAAGCTAAAGCAGCCGAGAACATCAAGGAAACATTTGGAGACATGCTGTTCTTTCCTGAAGCAGAATATCTACAAGAATTTCCTTCTCCAGAAGAATTGAAGTATCGGATCATCATTTCAACAAAACCCCCTAAAGAATATCTCAGATCCAAAAATGTGAAAGGGGAGAATTCTTCCAGTGAAGATGAAACGGATGATGAAGATGGGGTCGATACGAGTGAGAGTGAATCCAGCGAGAGTGACGACAGTGATTACGAATCTTACGCTACAGGTGTATCCGAATACAAACGCCTAATTGCCATTCATGCTGGCAAACCAAAGGGGGGATTGAAGGAAGCTCTAAAAGTTGGAGCTGATAAAGCTAGACGCCTTAGCTTGAGTGAAAAGGCACTAAAAAAGGCAACTAAATCTCATGGAATGGATGTCATTAGATTCACCCAGAAGAACATGTTAAGGATATATCCGAAGGGAACTCGAGTAAACTCTTCCAACTACAACCCAATGAGTGCTTGGTTACACGGGGCTCAGATGGTTGCTTTCAATATGCAGGGATACGGAAAGTTTCTCAGTTTAATGTACGGGATGTTTCGATCGAACGGAGGTTGTGGTTATGTGAAAAAACCTGATTTTCTACTGACAGTTGATCCGCATGGCAAGGTGAAAGTCTACATGGGTGTTGGCTGGAATTTGGATTTTGAAAGCACCCACTTTCATTTGTACTCCCCTCCTGACTTCTACGCACGGATTGGAATCGCAGGAGCTCCAGATGATGCGATAATAAAGAGAACAGAGATCAAAGAGGAGACTTGGAGCCCAGTTTGGAACGAAGAGTTTACATATCCTTTGACCATTCCTGAACTGGCTTTACTTCGAATCGAAGTGTATAGTTATAACATTTCAGATAAGGATGTTTTTGGAGGACAAAACTGTCTGCCTGTTCCTGAAGTGAAGGCAGGCATCCATGCTGTTCCTCTTTTTGACCGTAAAGGATCAAAGACAAATGCAGCAGAAGCACCAGAAGATGTGAAGATGATGTTCAATAAATACTCAAAACATGGCACAATGAACATTGATCAATTGCGGACGTTTTTGGAAGAGATTCAAGGAGAAACATCTGAGACAAAAGCTGAGGACATCTTCAACAGTCTCAAGCACCTCAACATCTTTCAGAGAAGAGGCCTTTATCTCGAAGATTTCTTCCGATATCTTCTTGGAGACCTTAATCTTGCTTTCCCGCACTGCCAAGGGGTTTATCAGGATATGGGAGCCCCATTGTCTCACTACTACATATTTACAGGCCACAATTCCTACTTAACAGGGAATCAACTGTGTAGTAAAAGCAGTGTGACTCCTATCATAAGGGCTCTAAAAAGAGGAGTAAGAGCAATTGAGTTGGACTTGTGGCCTACATCAAAGATGAATGGCATTGAGGTTCGTCATGGA

Coding sequence (CDS)

ATGGGAAATTACAGGGTTTGTCTCTGTTTCATCAGAAGGTTTAAGATGTCCACAGACGAGCCACCGTCGGACGTCAAGGAGGCGTTTCTCAAATACGCCAGCGCCGCCGCTGCCAACATGACCGTCGAGCAGTTCCACTCGTTTCTTGTGGAGTTTCAAGGAGATCAGACAGCGTCAATGGCCGATGCTCAACGGATTGTTGAGCAAGTGCTGCAGCGGCGGCATCATGTGATCTCCAAGCTCACAAAACACAGTCTCAATCTTGATGATTTCTATTACTATCTCTTCTCTATGGATCTCAATCCCCCAGTTTCAGATCAGGTTCATCAGGATATGAGAGCTCCACTGTCCCATTATTACATTTATACTAGCCACAATACATACTTAACTGGGAATCAATTGAGCAGTGATTCCAGTGATGTTCCGATCATAAATGCACTGAAAAGTGGTGTGAGAGTCGTGGAGCTTGATTTGTGGCCAAACTCTGAAGAAGATGACATAGATGTTCTTCATGGAAGGACCTTGACAACACCTGTGGAATTCGTTAGATGTCTGAAATCTATTAAAGAGAATGCTTTCATAGCATCTCCATATCCTGTCATAATTACTCTAGAAGACCACCTAACTCCTGATCTTCAAGCTAAAGCAGCCGAGAACATCAAGGAAACATTTGGAGACATGCTGTTCTTTCCTGAAGCAGAATATCTACAAGAATTTCCTTCTCCAGAAGAATTGAAGTATCGGATCATCATTTCAACAAAACCCCCTAAAGAATATCTCAGATCCAAAAATGTGAAAGGGGAGAATTCTTCCAGTGAAGATGAAACGGATGATGAAGATGGGGTCGATACGAGTGAGAGTGAATCCAGCGAGAGTGACGACAGTGATTACGAATCTTACGCTACAGGTGTATCCGAATACAAACGCCTAATTGCCATTCATGCTGGCAAACCAAAGGGGGGATTGAAGGAAGCTCTAAAAGTTGGAGCTGATAAAGCTAGACGCCTTAGCTTGAGTGAAAAGGCACTAAAAAAGGCAACTAAATCTCATGGAATGGATGTCATTAGATTCACCCAGAAGAACATGTTAAGGATATATCCGAAGGGAACTCGAGTAAACTCTTCCAACTACAACCCAATGAGTGCTTGGTTACACGGGGCTCAGATGGTTGCTTTCAATATGCAGGGATACGGAAAGTTTCTCAGTTTAATGTACGGGATGTTTCGATCGAACGGAGGTTGTGGTTATGTGAAAAAACCTGATTTTCTACTGACAGTTGATCCGCATGGCAAGGTGAAAGTCTACATGGGTGTTGGCTGGAATTTGGATTTTGAAAGCACCCACTTTCATTTGTACTCCCCTCCTGACTTCTACGCACGGATTGGAATCGCAGGAGCTCCAGATGATGCGATAATAAAGAGAACAGAGATCAAAGAGGAGACTTGGAGCCCAGTTTGGAACGAAGAGTTTACATATCCTTTGACCATTCCTGAACTGGCTTTACTTCGAATCGAAGTGTATAGTTATAACATTTCAGATAAGGATGTTTTTGGAGGACAAAACTGTCTGCCTGTTCCTGAAGTGAAGGCAGGCATCCATGCTGTTCCTCTTTTTGACCGTAAAGGATCAAAGACAAATGCAGCAGAAGCACCAGAAGATGTGAAGATGATGTTCAATAAATACTCAAAACATGGCACAATGAACATTGATCAATTGCGGACGTTTTTGGAAGAGATTCAAGGAGAAACATCTGAGACAAAAGCTGAGGACATCTTCAACAGTCTCAAGCACCTCAACATCTTTCAGAGAAGAGGCCTTTATCTCGAAGATTTCTTCCGATATCTTCTTGGAGACCTTAATCTTGCTTTCCCGCACTGCCAAGGGGTTTATCAGGATATGGGAGCCCCATTGTCTCACTACTACATATTTACAGGCCACAATTCCTACTTAACAGGGAATCAACTGTGTAGTAAAAGCAGTGTGACTCCTATCATAAGGGCTCTAAAAAGAGGAGTAAGAGCAATTGAGTTGGACTTGTGGCCTACATCAAAGATGAATGGCATTGAGGTTCGTCATGGA

Protein sequence

MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASMADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYYIYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEFVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFPSPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQKNMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGYVKKPDFLLTVDPHGKVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAGAPDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPVPEVKAGIHAVPLFDRKGSKTNAAEAPEDVKMMFNKYSKHGTMNIDQLRTFLEEIQGETSETKAEDIFNSLKHLNIFQRRGLYLEDFFRYLLGDLNLAFPHCQGVYQDMGAPLSHYYIFTGHNSYLTGNQLCSKSSVTPIIRALKRGVRAIELDLWPTSKMNGIEVRHG
Homology
BLAST of Carg12430 vs. NCBI nr
Match: KAG7036987.1 (Phosphoinositide phospholipase C 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1400.6 bits (3624), Expect = 0.0e+00
Identity = 693/693 (100.00%), Postives = 693/693 (100.00%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM
Sbjct: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
           ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
           FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300
           SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY
Sbjct: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300

Query: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360
           ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK
Sbjct: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360

Query: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGYVKKP 420
           NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGYVKKP
Sbjct: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGYVKKP 420

Query: 421 DFLLTVDPHGKVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAGAPDDAIIKRTEIKEE 480
           DFLLTVDPHGKVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAGAPDDAIIKRTEIKEE
Sbjct: 421 DFLLTVDPHGKVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAGAPDDAIIKRTEIKEE 480

Query: 481 TWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPVPEVKAGIHAVPLFDR 540
           TWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPVPEVKAGIHAVPLFDR
Sbjct: 481 TWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPVPEVKAGIHAVPLFDR 540

Query: 541 KGSKTNAAEAPEDVKMMFNKYSKHGTMNIDQLRTFLEEIQGETSETKAEDIFNSLKHLNI 600
           KGSKTNAAEAPEDVKMMFNKYSKHGTMNIDQLRTFLEEIQGETSETKAEDIFNSLKHLNI
Sbjct: 541 KGSKTNAAEAPEDVKMMFNKYSKHGTMNIDQLRTFLEEIQGETSETKAEDIFNSLKHLNI 600

Query: 601 FQRRGLYLEDFFRYLLGDLNLAFPHCQGVYQDMGAPLSHYYIFTGHNSYLTGNQLCSKSS 660
           FQRRGLYLEDFFRYLLGDLNLAFPHCQGVYQDMGAPLSHYYIFTGHNSYLTGNQLCSKSS
Sbjct: 601 FQRRGLYLEDFFRYLLGDLNLAFPHCQGVYQDMGAPLSHYYIFTGHNSYLTGNQLCSKSS 660

Query: 661 VTPIIRALKRGVRAIELDLWPTSKMNGIEVRHG 694
           VTPIIRALKRGVRAIELDLWPTSKMNGIEVRHG
Sbjct: 661 VTPIIRALKRGVRAIELDLWPTSKMNGIEVRHG 693

BLAST of Carg12430 vs. NCBI nr
Match: KAG7019134.1 (Phosphoinositide phospholipase C 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1227.2 bits (3174), Expect = 0.0e+00
Identity = 618/735 (84.08%), Postives = 652/735 (88.71%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MGNYRVCLCFIRRFKMS +EPP +VK+AF+KY    +ANMT EQ  SFLVEFQGDQTAS+
Sbjct: 1   MGNYRVCLCFIRRFKMSKEEPPPEVKDAFIKYTGGTSANMTAEQLRSFLVEFQGDQTASL 60

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
           ADAQRIVE VLQRRHHVISKLT+H+L LDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEHVLQRRHHVISKLTRHTLTLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSE+DDIDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEQDDIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
           FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDT------------SESE 300
           SPEELKYRIIISTKPPKEYLRSKNVKGE S S+DETDD+DGVDT            SESE
Sbjct: 241 SPEELKYRIIISTKPPKEYLRSKNVKGETSFSDDETDDDDGVDTYLDEVYNSIGLQSESE 300

Query: 301 SSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATK 360
           S+ESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGA K RRLSLSEKA+KKATK
Sbjct: 301 STESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGAAKVRRLSLSEKAVKKATK 360

Query: 361 SHGMDVIRFTQKNMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMF 420
           SHGMDVIRFTQ NMLRIYPKGTRVNSSNY P +AWLHGAQMVAFNMQG+G+ LSLM GMF
Sbjct: 361 SHGMDVIRFTQTNMLRIYPKGTRVNSSNYKPQTAWLHGAQMVAFNMQGHGRSLSLMRGMF 420

Query: 421 RSNGGCGYVKKPDFLLTVDPHG---------------KVKVYMGVGWNLDFESTHFHLYS 480
           RSNGGCGYVKKPDFLLTVDPHG               KVK+YMGVGWNLDFE +HFHLYS
Sbjct: 421 RSNGGCGYVKKPDFLLTVDPHGKVFDPNANLPVKKTMKVKIYMGVGWNLDFERSHFHLYS 480

Query: 481 PPDFYARIGIAGAPDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISD 540
           PPDFY RIGIAGAP D IIK+T+IKEETWSPVWN+EF++PLTIPELALLRIEVYSYNISD
Sbjct: 481 PPDFYTRIGIAGAPADEIIKKTDIKEETWSPVWNKEFSFPLTIPELALLRIEVYSYNISD 540

Query: 541 KDVFGGQNCLPVPEVKAGIHAVPLFDRKGSK---------------TNAAEAPEDVKMMF 600
           K+VFGGQNCLPVPEVK GIHAVPLFDRKGSK               TN AEAPEDVK +F
Sbjct: 541 KNVFGGQNCLPVPEVKPGIHAVPLFDRKGSKYSSVKLLMRFERRFRTNVAEAPEDVKRVF 600

Query: 601 NKYSKHGTMNIDQLRTFLEEIQGETSETKAEDIFNSLKHLNIFQRRGLYLEDFFRYLLGD 660
           ++YS++GTMNIDQLR FLEE+QGE SE KA  IF++LKHLNIFQRRGL LEDFFRYLLGD
Sbjct: 601 DQYSENGTMNIDQLRMFLEEVQGEESEMKAHAIFDNLKHLNIFQRRGLRLEDFFRYLLGD 660

Query: 661 LNLAFPHCQGVYQDMGAPLSHYYIFTGHNSYLTGNQLCSKSSVTPIIRALKRGVRAIELD 694
           LNLAF   QGVYQDM APLSHYYIFTGHNSYLTGNQL S SSVTPIIRALKRGVRAIELD
Sbjct: 661 LNLAFSPSQGVYQDMRAPLSHYYIFTGHNSYLTGNQLSSASSVTPIIRALKRGVRAIELD 720

BLAST of Carg12430 vs. NCBI nr
Match: XP_022948784.1 (phosphoinositide phospholipase C 4-like [Cucurbita moschata])

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 543/564 (96.28%), Postives = 546/564 (96.81%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM
Sbjct: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
           ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
           FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300
           SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY
Sbjct: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300

Query: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360
           ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK
Sbjct: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360

Query: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGYVKKP 420
           NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGK LSLMYGMFRSNGGCGYVKKP
Sbjct: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKSLSLMYGMFRSNGGCGYVKKP 420

Query: 421 DFLLTVDPHG---------------KVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG 480
           DFLLTVDPHG               KVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG
Sbjct: 421 DFLLTVDPHGKVFDPNAKLPVKKTLKVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG 480

Query: 481 APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV 540
           APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV
Sbjct: 481 APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV 540

Query: 541 PEVKAGIHAVPLFDRKGSKTNAAE 550
           PEVKAGIHAVPLFDRKGSK ++ +
Sbjct: 541 PEVKAGIHAVPLFDRKGSKYSSVK 564

BLAST of Carg12430 vs. NCBI nr
Match: XP_023523267.1 (phosphoinositide phospholipase C 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 542/564 (96.10%), Postives = 546/564 (96.81%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYA+AAAANMTVEQFHSFLVEFQGDQTASM
Sbjct: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYANAAAANMTVEQFHSFLVEFQGDQTASM 60

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
           ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
           FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300
           SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY
Sbjct: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300

Query: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360
           ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK
Sbjct: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360

Query: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGYVKKP 420
           NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGK LSLMYGMFRSNGGCGYVKKP
Sbjct: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKSLSLMYGMFRSNGGCGYVKKP 420

Query: 421 DFLLTVDPHG---------------KVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG 480
           DFLLTVDPHG               KVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG
Sbjct: 421 DFLLTVDPHGKVFDPNAKLPVKKTLKVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG 480

Query: 481 APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV 540
           APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV
Sbjct: 481 APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV 540

Query: 541 PEVKAGIHAVPLFDRKGSKTNAAE 550
           PEVKAGIHAVPLFDRKGSK ++ +
Sbjct: 541 PEVKAGIHAVPLFDRKGSKYSSVK 564

BLAST of Carg12430 vs. NCBI nr
Match: XP_022998188.1 (phosphoinositide phospholipase C 4-like [Cucurbita maxima])

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 540/564 (95.74%), Postives = 543/564 (96.28%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYAS AAANMTVEQFHSFLVEFQGDQTASM
Sbjct: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASVAAANMTVEQFHSFLVEFQGDQTASM 60

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
           ADAQRIVEQVLQRRHHVISKLTKH LNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEQVLQRRHHVISKLTKHGLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
           FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300
           SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY
Sbjct: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300

Query: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360
           ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK
Sbjct: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360

Query: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGYVKKP 420
           NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGK LSLMYGMFRSNGGCGYVKKP
Sbjct: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKSLSLMYGMFRSNGGCGYVKKP 420

Query: 421 DFLLTVDPHG---------------KVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG 480
           DFLLTVDPHG               KVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG
Sbjct: 421 DFLLTVDPHGKVFDPNAKLPVKKTLKVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG 480

Query: 481 APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV 540
           APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV
Sbjct: 481 APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV 540

Query: 541 PEVKAGIHAVPLFDRKGSKTNAAE 550
           PEVKAGIHAVPLFDRKG K ++ +
Sbjct: 541 PEVKAGIHAVPLFDRKGLKYSSVK 564

BLAST of Carg12430 vs. ExPASy Swiss-Prot
Match: Q944C1 (Phosphoinositide phospholipase C 4 OS=Arabidopsis thaliana OX=3702 GN=PLC4 PE=2 SV=2)

HSP 1 Score: 643.3 bits (1658), Expect = 3.2e-183
Identity = 329/582 (56.53%), Postives = 421/582 (72.34%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MG+Y+ CL F R+F+M+   P  DV++ F KY +   A+M+ EQ    + E  G+   S+
Sbjct: 7   MGSYKFCLIFTRKFRMTESGPVEDVRDLFEKY-TEGDAHMSPEQLQKLMTEEGGEGETSL 66

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
            +A+RIV++VL+R+HH I+K T+ +L LDDF YYLFS DLNPP++DQVHQ+M APLSHY+
Sbjct: 67  EEAERIVDEVLRRKHH-IAKFTRRNLTLDDFNYYLFSTDLNPPIADQVHQNMDAPLSHYF 126

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           I+T HN+YLTGNQLSS+ S++PI +AL+ GVRVVELDLWP    DD+ V HGRTLT  V+
Sbjct: 127 IFTGHNSYLTGNQLSSNCSELPIADALRRGVRVVELDLWPRG-TDDVCVKHGRTLTKEVK 186

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
             +CL+SIK NAF  S YPVIITLEDHLTP LQ K A+ I +TFGDML++ +++  QEFP
Sbjct: 187 LGKCLESIKANAFAISKYPVIITLEDHLTPKLQFKVAKMITQTFGDMLYYHDSQGCQEFP 246

Query: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300
           SPEELK +I+ISTKPPKEYL + + K +++  + +  DED V   E E   S  SD +  
Sbjct: 247 SPEELKEKILISTKPPKEYLEANDTKEKDNGEKGKDSDED-VWGKEPEDLISTQSDLDKV 306

Query: 301 ATGVS-----------------------EYKRLIAIHAGKPKGGLKEALKVGADKARRLS 360
            + V+                       EYKRLIAIHAGKPKGGL+ ALKV  +K RRLS
Sbjct: 307 TSSVNDLNQDDEERGSCESDTSCQLQAPEYKRLIAIHAGKPKGGLRMALKVDPNKIRRLS 366

Query: 361 LSEKALKKATKSHGMDVIRFTQKNMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGY 420
           LSE+ L+KA  S+G DVIRFTQKN LRIYPKGTR NSSNY P   W+ GAQM+AFNMQGY
Sbjct: 367 LSEQLLEKAVASYGADVIRFTQKNFLRIYPKGTRFNSSNYKPQIGWMSGAQMIAFNMQGY 426

Query: 421 GKFLSLMYGMFRSNGGCGYVKKPDFLLTVDPHG---------------KVKVYMGVGWNL 480
           G+ L LM GMFR+NGGCGYVKKPDFL+   P+G               KVKV MG GW L
Sbjct: 427 GRALWLMEGMFRANGGCGYVKKPDFLMDASPNGQDFYPKDNSSPKKTLKVKVCMGDGWLL 486

Query: 481 DFESTHFHLYSPPDFYARIGIAGAPDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALL 540
           DF+ THF  YSPPDF+ R+GIAGAP D ++++T+I+ +TW+P+WN+EFT+PL +PELALL
Sbjct: 487 DFKKTHFDSYSPPDFFVRVGIAGAPVDEVMEKTKIEYDTWTPIWNKEFTFPLAVPELALL 546

Query: 541 RIEVYSYNISDKDVFGGQNCLPVPEVKAGIHAVPLFDRKGSK 545
           R+EV+ +++++KD FGGQ CLPV E++ GI AVPLF+RKG K
Sbjct: 547 RVEVHEHDVNEKDDFGGQTCLPVSEIRQGIRAVPLFNRKGVK 584

BLAST of Carg12430 vs. ExPASy Swiss-Prot
Match: Q8GV43 (Phosphoinositide phospholipase C 6 OS=Arabidopsis thaliana OX=3702 GN=PLC6 PE=2 SV=2)

HSP 1 Score: 632.5 bits (1630), Expect = 5.7e-180
Identity = 330/586 (56.31%), Postives = 418/586 (71.33%), Query Frame = 0

Query: 3   NYRVCLCFIRRFKMSTDEPPSDVKEAFLKYA---------------SAAAANMTVEQFHS 62
           NYR+   + R+FK++   P  DV++AF ++A                 +   M  EQ  S
Sbjct: 18  NYRMFKFYNRKFKINEVTPTDDVRDAFCQFAVGGGGGGTDGDSSDGDGSTGVMGAEQLCS 77

Query: 63  FLVEFQGDQTASMADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQ 122
           FL +  G+ T ++A+AQR++++V++RRHHV ++ T+H L+LDDF+ +LF  DLNPP++  
Sbjct: 78  FL-DDHGEST-TVAEAQRLIDEVIRRRHHV-TRFTRHGLDLDDFFNFLFYDDLNPPITPH 137

Query: 123 VHQDMRAPLSHYYIYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDI 182
           VHQDM APLSHY+IYT HN+YLTGNQLSSD S+VP+I AL+ GVRV+ELDLWPNS   DI
Sbjct: 138 VHQDMTAPLSHYFIYTGHNSYLTGNQLSSDCSEVPVIKALQRGVRVIELDLWPNSTGTDI 197

Query: 183 DVLHGRTLTTPVEFVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDM 242
           +VLHGRTLTTPV  ++CLKSI++ AF +SPYPVIITLEDHLTPDLQAK AE   + FG M
Sbjct: 198 NVLHGRTLTTPVPLMKCLKSIRDYAFSSSPYPVIITLEDHLTPDLQAKVAEMATQIFGQM 257

Query: 243 LFFPEAEYLQEFPSPEELKYRIIISTKPPKEYLRSKN--VKGENS----SSEDETDDEDG 302
           L++PE++ L EFPSP  L +RIIISTKPPKEYL S+N  VK +++    SSEDET   + 
Sbjct: 258 LYYPESDSLLEFPSPASLLHRIIISTKPPKEYLESRNPIVKQKDNNVSPSSEDETPRTEE 317

Query: 303 VDTSES--------ESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKA 362
           + T ES          S+SD  D E+       YKRLI IHAGKPKG +KE +KV  DK 
Sbjct: 318 IQTLESMLFDQDFESKSDSDQEDEEASEDQKPAYKRLITIHAGKPKGTVKEEMKVVVDKV 377

Query: 363 RRLSLSEKALKKATKSHGMDVIRFTQKNMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFN 422
           RRLSLSE+ L +   S+  DV+RFTQ+N+LRIYPKGTR NSSNY P+  W HGAQM+AFN
Sbjct: 378 RRLSLSEQELDRTCSSNSQDVVRFTQRNLLRIYPKGTRFNSSNYKPLIGWTHGAQMIAFN 437

Query: 423 MQGYGKFLSLMYGMFRSNGGCGYVKKPDFLL-------TVDPHG--------KVKVYMGV 482
           MQGYGK L LM+GMFR+NGGCGYVKKP+FL+         DP          KVKVYMG 
Sbjct: 438 MQGYGKSLWLMHGMFRANGGCGYVKKPNFLMKKGFHDEVFDPRKKLPVKETLKVKVYMGD 497

Query: 483 GWNLDFESTHFHLYSPPDFYARIGIAGAPDDAIIKRTEIKEETWSPVWNEEFTYPLTIPE 542
           GW +DF  THF  YSPPDFY ++ I G P D   K+T+I E+ W P+W+EEF++PLT+PE
Sbjct: 498 GWRMDFSHTHFDAYSPPDFYTKMFIVGVPADNAKKKTKIIEDNWYPIWDEEFSFPLTVPE 557

Query: 543 LALLRIEVYSYNISDKDVFGGQNCLPVPEVKAGIHAVPLFDRKGSK 545
           LALLRIEV  Y++S+KD FGGQ CLPV E++ GI +VPL+D+KG K
Sbjct: 558 LALLRIEVREYDMSEKDDFGGQTCLPVAELRPGIRSVPLYDKKGEK 600

BLAST of Carg12430 vs. ExPASy Swiss-Prot
Match: Q944C2 (Phosphoinositide phospholipase C 5 OS=Arabidopsis thaliana OX=3702 GN=PLC5 PE=2 SV=2)

HSP 1 Score: 615.5 bits (1586), Expect = 7.2e-175
Identity = 311/563 (55.24%), Postives = 405/563 (71.94%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MG+Y++ LC   + +M+   PP DV  AF++Y +   ++MT EQ   FLVE Q +    +
Sbjct: 5   MGSYKMGLCCSDKLRMNRGAPPQDVVTAFVEY-TEGRSHMTAEQLCRFLVEVQDETEVLV 64

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
           +DA++I+E++   RHH I+K  +H+LNLDDF+ +LFS DLN P+  +VHQDM +PLSHY+
Sbjct: 65  SDAEKIIERITCERHH-ITKFLRHTLNLDDFFSFLFSDDLNHPIDSKVHQDMASPLSHYF 124

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           IYTSHN+YLTGNQ++S+ SDVP+I ALK GVR +ELD+WPNS +DDI VLHG   T PVE
Sbjct: 125 IYTSHNSYLTGNQINSECSDVPLIKALKRGVRALELDMWPNSTKDDILVLHGWAWTPPVE 184

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
            V+CL+SIKE+AF AS YPVI+TLEDHLTPDLQAKAAE +KE F DM++FPEA  L+EFP
Sbjct: 185 LVKCLRSIKEHAFYASAYPVILTLEDHLTPDLQAKAAEMMKEIFMDMVYFPEAGGLKEFP 244

Query: 241 SPEELKYRIIISTKPPKEYLR----SKNVKGENSSSEDETDDEDGVDTSESESSESDDSD 300
           SPE+LKY+I+ISTKPPK  LR    S++     +SS+   DDE   + +    +E D  D
Sbjct: 245 SPEDLKYKIVISTKPPKGSLRKDKDSESDASGKASSDVSADDEKTEEETSEAKNEEDGFD 304

Query: 301 YESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIR 360
            ES       Y RLI I +G  K GLKEAL +     RRLSL E+  KKAT+ +G +VI+
Sbjct: 305 QESSNLDFLTYSRLITIPSGNAKNGLKEALTIDNGGVRRLSLREQKFKKATEMYGTEVIK 364

Query: 361 FTQKNMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGY 420
           FTQKN+LRIYPK TRVNSSNY P + W++GAQMVAFNMQGYG+ L +M+GMFR NGGCGY
Sbjct: 365 FTQKNLLRIYPKATRVNSSNYRPYNGWMYGAQMVAFNMQGYGRALWMMHGMFRGNGGCGY 424

Query: 421 VKKPDFLLTVDPHG---------------KVKVYMGVGWNLDFESTHFHLYSPPDFYARI 480
           VKKPDF++  +  G               KVKVYMG GW+  F+ T F+ +S P+FY R+
Sbjct: 425 VKKPDFMMNNNLSGEVFNPKAKLPIKKTLKVKVYMGKGWDSGFQRTCFNTWSSPNFYTRV 484

Query: 481 GIAGAPDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQN 540
           GI G   D ++K+T+ +++TW P WNEEF + LT+PELALLRIEV+ YN+ +KD F GQ 
Sbjct: 485 GITGVRGDKVMKKTKKEQKTWEPFWNEEFEFQLTVPELALLRIEVHDYNMPEKDDFSGQT 544

Query: 541 CLPVPEVKAGIHAVPLFDRKGSK 545
           CLPV E++ GI +VPL+DRKG +
Sbjct: 545 CLPVSELRQGIRSVPLYDRKGER 565

BLAST of Carg12430 vs. ExPASy Swiss-Prot
Match: Q39033 (Phosphoinositide phospholipase C 2 OS=Arabidopsis thaliana OX=3702 GN=PLC2 PE=1 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 2.0e-169
Identity = 311/573 (54.28%), Postives = 389/573 (67.89%), Query Frame = 0

Query: 4   YRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASMADA 63
           Y+VC CF RRF+ +  E P ++K  F KY+      MTV+  H FL++ Q    A+  DA
Sbjct: 6   YKVCFCFRRRFRYTASEAPREIKTIFEKYSENGV--MTVDHLHRFLIDVQKQDKATREDA 65

Query: 64  QRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVS-DQVHQDMRAPLSHYYIY 123
           Q I+       +   S L ++ L+LD F+ YLF  D NPP++  +VH DM AP+SHY+I+
Sbjct: 66  QSII-------NSASSLLHRNGLHLDAFFKYLFG-DNNPPLALHKVHHDMDAPISHYFIF 125

Query: 124 TSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEFV 183
           T HN+YLTGNQLSSD S+VPII+ALK GVRV+ELD+WPNS +DDIDVLHG TLTTPV  +
Sbjct: 126 TGHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGLI 185

Query: 184 RCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPE-AEYLQEFPS 243
           +CLK+I+ +AF  S YPV++TLEDHLTPDLQ+K AE + E FG++LF P   E L+EFPS
Sbjct: 186 KCLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFPS 245

Query: 244 PEELKYRIIISTKPPKEYLRSKNV----KGENSSSED-----------ETDDEDGVDTSE 303
           P  LK RIIISTKPPKEY   K+V    KG++   E+               E   D   
Sbjct: 246 PNSLKRRIIISTKPPKEYKEGKDVEVVQKGKDLGDEEVWGREVPSFIQRNKSEAKDDLDG 305

Query: 304 SESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKA 363
           ++  + DD + +S      +YK LIAIHAGKPKGG+ E LKV  DK RRLSLSE+ L+KA
Sbjct: 306 NDDDDDDDDEDKSKINAPPQYKHLIAIHAGKPKGGITECLKVDPDKVRRLSLSEEQLEKA 365

Query: 364 TKSHGMDVIRFTQKNMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYG 423
            + +   ++RFTQ N+LRIYPKGTRV SSNYNP+  W HGAQMVAFNMQGYG+ L LM G
Sbjct: 366 AEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRSLWLMQG 425

Query: 424 MFRSNGGCGYVKKPDFLL-------TVDPHG--------KVKVYMGVGWNLDFESTHFHL 483
           MFR+NGGCGY+KKPD LL         DP          +V VYMG GW  DF  THF  
Sbjct: 426 MFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFRHTHFDQ 485

Query: 484 YSPPDFYARIGIAGAPDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNI 543
           YSPPDFY R+GIAG P D ++K+T+  E+ W P W+E F +PLT+PELALLR+EV+ Y++
Sbjct: 486 YSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLEVHEYDM 545

Query: 544 SDKDVFGGQNCLPVPEVKAGIHAVPLFDRKGSK 545
           S+KD FGGQ CLPV E+  GI A PL  RKG K
Sbjct: 546 SEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEK 568

BLAST of Carg12430 vs. ExPASy Swiss-Prot
Match: Q9LY51 (Phosphoinositide phospholipase C 7 OS=Arabidopsis thaliana OX=3702 GN=PLC7 PE=1 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 9.7e-164
Identity = 303/580 (52.24%), Postives = 388/580 (66.90%), Query Frame = 0

Query: 4   YRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASMADA 63
           Y+VC CF RR++ +    P+++K  F  Y+      MT +    FL++ Q    A+  +A
Sbjct: 6   YKVCFCFRRRYRHTVSVAPAEIKTLFDNYSDKGL--MTTDLLLRFLIDVQKQDKATKEEA 65

Query: 64  QRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYYIYT 123
           Q IV       +   S L ++ L+LD F+ YLF++  +P  S +VHQDM APLSHY+IYT
Sbjct: 66  QDIV-------NASSSLLHRNGLHLDAFFKYLFAVTNSPLSSLEVHQDMDAPLSHYFIYT 125

Query: 124 SHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEFVR 183
            HN+YLTGNQLSSD S++PII ALK GVRV+ELD+WPNS+ED IDVLHGRTLT+PVE ++
Sbjct: 126 GHNSYLTGNQLSSDCSELPIIEALKKGVRVIELDIWPNSDEDGIDVLHGRTLTSPVELIK 185

Query: 184 CLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFF-PEAEYLQEFPSP 243
           CL++I+E+AF  S YPV++TLEDHLTP LQAK AE + + FG+MLF  P  E L+EFPSP
Sbjct: 186 CLRAIREHAFDVSDYPVVVTLEDHLTPKLQAKVAEMVTDIFGEMLFTPPSGECLKEFPSP 245

Query: 244 EELKYRIIISTKPPKEY--------------LRSKNVKG-------ENSSSEDETDDEDG 303
             LK RI+ISTKPPKEY              L  K V G           S D+ D    
Sbjct: 246 AFLKKRIMISTKPPKEYKAATDDDLVKKGRDLGDKEVWGREVPSFIRRDRSVDKNDSNGD 305

Query: 304 VDTSESESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEK 363
            D  + +  + DD D +       EYK LIAI AGKPKGG+ E LKV  DK RRLSLSE+
Sbjct: 306 DDDDDDDDDDDDDGDDKIKKNAPPEYKHLIAIEAGKPKGGITECLKVDPDKVRRLSLSEE 365

Query: 364 ALKKATKSHGMDVIRFTQKNMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFL 423
            L+KA++ +   ++RFTQ+N+LR+YPKGTR+ SSNYNP+ AW HGAQMVAFNMQG G+ L
Sbjct: 366 QLEKASEKYAKQIVRFTQRNLLRVYPKGTRITSSNYNPLIAWSHGAQMVAFNMQGLGRSL 425

Query: 424 SLMYGMFRSNGGCGYVKKPDFLL----TVDPHG--------KVKVYMGVGWNLDFESTHF 483
            +M GMFR NGGCGY+KKPD LL      DP          +V +YMG GW  DF  THF
Sbjct: 426 WVMQGMFRGNGGCGYIKKPDLLLKSNAVFDPEATLPVKTTLRVTIYMGEGWYYDFPHTHF 485

Query: 484 HLYSPPDFYARIGIAGAPDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSY 543
             YSPPDFY R+GIAG P D ++K+T+  E+ W P W+E F +PLT+PELALLRIEV+ Y
Sbjct: 486 DRYSPPDFYTRVGIAGVPADTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRIEVHEY 545

Query: 544 NISDKDVFGGQNCLPVPEVKAGIHAVPLFDRKGSKTNAAE 550
           ++S+KD FGGQ CLPV E++ GI AVPL ++ G K  + +
Sbjct: 546 DMSEKDDFGGQICLPVWELRQGIRAVPLRNQDGVKCRSVK 576

BLAST of Carg12430 vs. ExPASy TrEMBL
Match: A0A6J1GAY7 (Phosphoinositide phospholipase C OS=Cucurbita moschata OX=3662 GN=LOC111452349 PE=4 SV=1)

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 543/564 (96.28%), Postives = 546/564 (96.81%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM
Sbjct: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
           ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
           FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300
           SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY
Sbjct: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300

Query: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360
           ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK
Sbjct: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360

Query: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGYVKKP 420
           NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGK LSLMYGMFRSNGGCGYVKKP
Sbjct: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKSLSLMYGMFRSNGGCGYVKKP 420

Query: 421 DFLLTVDPHG---------------KVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG 480
           DFLLTVDPHG               KVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG
Sbjct: 421 DFLLTVDPHGKVFDPNAKLPVKKTLKVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG 480

Query: 481 APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV 540
           APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV
Sbjct: 481 APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV 540

Query: 541 PEVKAGIHAVPLFDRKGSKTNAAE 550
           PEVKAGIHAVPLFDRKGSK ++ +
Sbjct: 541 PEVKAGIHAVPLFDRKGSKYSSVK 564

BLAST of Carg12430 vs. ExPASy TrEMBL
Match: A0A6J1KG31 (Phosphoinositide phospholipase C OS=Cucurbita maxima OX=3661 GN=LOC111492913 PE=4 SV=1)

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 540/564 (95.74%), Postives = 543/564 (96.28%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYAS AAANMTVEQFHSFLVEFQGDQTASM
Sbjct: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASVAAANMTVEQFHSFLVEFQGDQTASM 60

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
           ADAQRIVEQVLQRRHHVISKLTKH LNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEQVLQRRHHVISKLTKHGLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
           FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300
           SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY
Sbjct: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300

Query: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360
           ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK
Sbjct: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360

Query: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGYVKKP 420
           NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGK LSLMYGMFRSNGGCGYVKKP
Sbjct: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKSLSLMYGMFRSNGGCGYVKKP 420

Query: 421 DFLLTVDPHG---------------KVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG 480
           DFLLTVDPHG               KVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG
Sbjct: 421 DFLLTVDPHGKVFDPNAKLPVKKTLKVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG 480

Query: 481 APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV 540
           APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV
Sbjct: 481 APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV 540

Query: 541 PEVKAGIHAVPLFDRKGSKTNAAE 550
           PEVKAGIHAVPLFDRKG K ++ +
Sbjct: 541 PEVKAGIHAVPLFDRKGLKYSSVK 564

BLAST of Carg12430 vs. ExPASy TrEMBL
Match: A0A1S3C6M6 (Phosphoinositide phospholipase C OS=Cucumis melo OX=3656 GN=LOC103497078 PE=4 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 4.1e-290
Identity = 500/564 (88.65%), Postives = 522/564 (92.55%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MGNYRVCLCFIRRFKMS +EPP DVKEAF+KYA   AANMT EQ  SFLVEFQGDQTAS+
Sbjct: 1   MGNYRVCLCFIRRFKMSKEEPPPDVKEAFVKYAGGVAANMTAEQLRSFLVEFQGDQTASL 60

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
           ADAQRIVE VLQRRHHVI+KLTKHSL LDDFYYYLFSMDLNPP+SDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEHVLQRRHHVITKLTKHSLTLDDFYYYLFSMDLNPPISDQVHQDMRAPLSHYY 120

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           IYTSHNTYLTGNQLSS+SSDVPIINALKSGVRVVELDLWPNSEED+IDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSNSSDVPIINALKSGVRVVELDLWPNSEEDEIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
           FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAE IKETFGDMLFFPEAEYLQEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAEYIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300
           SPEELKYRI+ISTKPPKEYLRSKNVKGENS S+DETDDEDGVDTSESESSESDDSDYESY
Sbjct: 241 SPEELKYRIVISTKPPKEYLRSKNVKGENSFSDDETDDEDGVDTSESESSESDDSDYESY 300

Query: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360
            TGVSEYKRLIAIHAGKP GGLKEALKVGADK RRLSLSEKALKKATK+HGMDVIRFTQK
Sbjct: 301 TTGVSEYKRLIAIHAGKPNGGLKEALKVGADKVRRLSLSEKALKKATKAHGMDVIRFTQK 360

Query: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGYVKKP 420
           NMLRIYPKGTRVNSSNY P +AWLHGAQMVAFNMQG+GK LSLM GMFRSNGGCGYVKKP
Sbjct: 361 NMLRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVKKP 420

Query: 421 DFLLTVDPHG---------------KVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG 480
           DFL+TVD HG               KVK+YMGVGWNLDFE+THFHLYSPPDFY RIGIAG
Sbjct: 421 DFLITVDSHGKVFDPNANLPVKRTLKVKLYMGVGWNLDFENTHFHLYSPPDFYTRIGIAG 480

Query: 481 APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV 540
           AP D +IK+TEIKEETWSPVWNEEFT+PLTIPELALLRI+VYSYNISDKDVFGGQNCLPV
Sbjct: 481 APADEVIKKTEIKEETWSPVWNEEFTFPLTIPELALLRIDVYSYNISDKDVFGGQNCLPV 540

Query: 541 PEVKAGIHAVPLFDRKGSKTNAAE 550
            EVK GIHAVPLFDRKGSK ++ +
Sbjct: 541 SEVKPGIHAVPLFDRKGSKYSSVK 564

BLAST of Carg12430 vs. ExPASy TrEMBL
Match: A0A6J1I2U5 (Phosphoinositide phospholipase C OS=Cucurbita maxima OX=3661 GN=LOC111469413 PE=4 SV=1)

HSP 1 Score: 1005.7 bits (2599), Expect = 9.1e-290
Identity = 498/564 (88.30%), Postives = 525/564 (93.09%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MGNYRVCLCFIRRFKMS +EPP +VK+AF+KY    +ANMT EQ  SFLVEFQGDQTAS+
Sbjct: 1   MGNYRVCLCFIRRFKMSKEEPPPEVKDAFIKYTGGTSANMTAEQLRSFLVEFQGDQTASL 60

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
           ADAQRIVE VLQRRHHVISKLT+H+L LDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEHVLQRRHHVISKLTRHTLTLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSE+DDIDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEQDDIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
           FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300
           SPEELKYRIIISTKPPKEYLRSKNVKGENSSS+DETDD+DGVDTSESESSESDDSDYESY
Sbjct: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSDDETDDDDGVDTSESESSESDDSDYESY 300

Query: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360
           ATGVSEYKRLIAIHAGKPKGGLKEALKVGA K RRLSLSEKA+KKATKSHGMDVIRFTQ 
Sbjct: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGAAKVRRLSLSEKAVKKATKSHGMDVIRFTQT 360

Query: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGYVKKP 420
           NMLRIYPKGTRVNSSNY P +AWLHGAQMVAFNMQG+G+ LSLM GMF+SNGGCGYVKKP
Sbjct: 361 NMLRIYPKGTRVNSSNYKPQTAWLHGAQMVAFNMQGHGRSLSLMRGMFQSNGGCGYVKKP 420

Query: 421 DFLLTVDPHG---------------KVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG 480
           DFLLTVDPHG               KVK+YMGVGWNLDFE +HFHLYSPPDFYARIGIAG
Sbjct: 421 DFLLTVDPHGKVFDPNANLPVKKTMKVKIYMGVGWNLDFERSHFHLYSPPDFYARIGIAG 480

Query: 481 APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV 540
           AP D IIK+T+IKEETWSPVWN+EF++PLTIPELALLRIEVYSYNISDK+VFGGQNCLPV
Sbjct: 481 APADEIIKKTDIKEETWSPVWNKEFSFPLTIPELALLRIEVYSYNISDKNVFGGQNCLPV 540

Query: 541 PEVKAGIHAVPLFDRKGSKTNAAE 550
           PEVK GIHAVPLFDRKGSK ++ +
Sbjct: 541 PEVKPGIHAVPLFDRKGSKYSSVK 564

BLAST of Carg12430 vs. ExPASy TrEMBL
Match: A0A0A0LVI7 (Phosphoinositide phospholipase C OS=Cucumis sativus OX=3659 GN=Csa_1G531180 PE=4 SV=1)

HSP 1 Score: 1001.1 bits (2587), Expect = 2.2e-288
Identity = 499/564 (88.48%), Postives = 519/564 (92.02%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MGNYRVCLCFIRRFKMS +EPP DVKEAF+KYA   AANMT E   SFLVEFQGDQTAS+
Sbjct: 1   MGNYRVCLCFIRRFKMSKEEPPPDVKEAFVKYAGGVAANMTAEHLRSFLVEFQGDQTASL 60

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
           ADAQRIVE VLQRRHHVI+KLTKH L LDDFYYYLFSMDLNP +SDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEHVLQRRHHVITKLTKHCLTLDDFYYYLFSMDLNPSISDQVHQDMRAPLSHYY 120

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEED+IDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDEIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
           FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAE IKETFGDMLFFPEAEYLQEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAEYIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300
           SPEELKYRIIISTKPPKEYL+SKNVKGENS S+DETDDEDGVDTSESESSESDDSDYESY
Sbjct: 241 SPEELKYRIIISTKPPKEYLKSKNVKGENSFSDDETDDEDGVDTSESESSESDDSDYESY 300

Query: 301 ATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIRFTQK 360
            TGVSEYKRLIAIHAGKPKGGLKEALKVGADK RRLSLSEKALKKATK+HG DVIRFTQK
Sbjct: 301 TTGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKAHGKDVIRFTQK 360

Query: 361 NMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGYVKKP 420
           NMLRIYPKGTRVNSSNY P +AWLHGAQMVAFNMQG+GK LSLM GMFRSNGGCGYVKKP
Sbjct: 361 NMLRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVKKP 420

Query: 421 DFLLTVDPHG---------------KVKVYMGVGWNLDFESTHFHLYSPPDFYARIGIAG 480
           DFLLTVD HG               KVKVYMGVGWNLDFE+THFHLYSPPDFY +IGIAG
Sbjct: 421 DFLLTVDAHGKVFDPTANLPVKRTLKVKVYMGVGWNLDFENTHFHLYSPPDFYTKIGIAG 480

Query: 481 APDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQNCLPV 540
           AP D +IK+TEIKEETWSPVWNEEFT+PLTIPELALLRI+VYSYNISDKDVFGGQNCLPV
Sbjct: 481 APADELIKKTEIKEETWSPVWNEEFTFPLTIPELALLRIDVYSYNISDKDVFGGQNCLPV 540

Query: 541 PEVKAGIHAVPLFDRKGSKTNAAE 550
            EVK GIHAVPLFDRKGSK ++ +
Sbjct: 541 SEVKPGIHAVPLFDRKGSKYSSVK 564

BLAST of Carg12430 vs. TAIR 10
Match: AT5G58700.1 (phosphatidylinositol-speciwc phospholipase C4 )

HSP 1 Score: 643.3 bits (1658), Expect = 2.3e-184
Identity = 329/582 (56.53%), Postives = 421/582 (72.34%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MG+Y+ CL F R+F+M+   P  DV++ F KY +   A+M+ EQ    + E  G+   S+
Sbjct: 7   MGSYKFCLIFTRKFRMTESGPVEDVRDLFEKY-TEGDAHMSPEQLQKLMTEEGGEGETSL 66

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
            +A+RIV++VL+R+HH I+K T+ +L LDDF YYLFS DLNPP++DQVHQ+M APLSHY+
Sbjct: 67  EEAERIVDEVLRRKHH-IAKFTRRNLTLDDFNYYLFSTDLNPPIADQVHQNMDAPLSHYF 126

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           I+T HN+YLTGNQLSS+ S++PI +AL+ GVRVVELDLWP    DD+ V HGRTLT  V+
Sbjct: 127 IFTGHNSYLTGNQLSSNCSELPIADALRRGVRVVELDLWPRG-TDDVCVKHGRTLTKEVK 186

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
             +CL+SIK NAF  S YPVIITLEDHLTP LQ K A+ I +TFGDML++ +++  QEFP
Sbjct: 187 LGKCLESIKANAFAISKYPVIITLEDHLTPKLQFKVAKMITQTFGDMLYYHDSQGCQEFP 246

Query: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSSSEDETDDEDGVDTSESESSESDDSDYESY 300
           SPEELK +I+ISTKPPKEYL + + K +++  + +  DED V   E E   S  SD +  
Sbjct: 247 SPEELKEKILISTKPPKEYLEANDTKEKDNGEKGKDSDED-VWGKEPEDLISTQSDLDKV 306

Query: 301 ATGVS-----------------------EYKRLIAIHAGKPKGGLKEALKVGADKARRLS 360
            + V+                       EYKRLIAIHAGKPKGGL+ ALKV  +K RRLS
Sbjct: 307 TSSVNDLNQDDEERGSCESDTSCQLQAPEYKRLIAIHAGKPKGGLRMALKVDPNKIRRLS 366

Query: 361 LSEKALKKATKSHGMDVIRFTQKNMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGY 420
           LSE+ L+KA  S+G DVIRFTQKN LRIYPKGTR NSSNY P   W+ GAQM+AFNMQGY
Sbjct: 367 LSEQLLEKAVASYGADVIRFTQKNFLRIYPKGTRFNSSNYKPQIGWMSGAQMIAFNMQGY 426

Query: 421 GKFLSLMYGMFRSNGGCGYVKKPDFLLTVDPHG---------------KVKVYMGVGWNL 480
           G+ L LM GMFR+NGGCGYVKKPDFL+   P+G               KVKV MG GW L
Sbjct: 427 GRALWLMEGMFRANGGCGYVKKPDFLMDASPNGQDFYPKDNSSPKKTLKVKVCMGDGWLL 486

Query: 481 DFESTHFHLYSPPDFYARIGIAGAPDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALL 540
           DF+ THF  YSPPDF+ R+GIAGAP D ++++T+I+ +TW+P+WN+EFT+PL +PELALL
Sbjct: 487 DFKKTHFDSYSPPDFFVRVGIAGAPVDEVMEKTKIEYDTWTPIWNKEFTFPLAVPELALL 546

Query: 541 RIEVYSYNISDKDVFGGQNCLPVPEVKAGIHAVPLFDRKGSK 545
           R+EV+ +++++KD FGGQ CLPV E++ GI AVPLF+RKG K
Sbjct: 547 RVEVHEHDVNEKDDFGGQTCLPVSEIRQGIRAVPLFNRKGVK 584

BLAST of Carg12430 vs. TAIR 10
Match: AT2G40116.1 (Phosphoinositide-specific phospholipase C family protein )

HSP 1 Score: 632.5 bits (1630), Expect = 4.0e-181
Identity = 330/586 (56.31%), Postives = 418/586 (71.33%), Query Frame = 0

Query: 3   NYRVCLCFIRRFKMSTDEPPSDVKEAFLKYA---------------SAAAANMTVEQFHS 62
           NYR+   + R+FK++   P  DV++AF ++A                 +   M  EQ  S
Sbjct: 18  NYRMFKFYNRKFKINEVTPTDDVRDAFCQFAVGGGGGGTDGDSSDGDGSTGVMGAEQLCS 77

Query: 63  FLVEFQGDQTASMADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQ 122
           FL +  G+ T ++A+AQR++++V++RRHHV ++ T+H L+LDDF+ +LF  DLNPP++  
Sbjct: 78  FL-DDHGEST-TVAEAQRLIDEVIRRRHHV-TRFTRHGLDLDDFFNFLFYDDLNPPITPH 137

Query: 123 VHQDMRAPLSHYYIYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDI 182
           VHQDM APLSHY+IYT HN+YLTGNQLSSD S+VP+I AL+ GVRV+ELDLWPNS   DI
Sbjct: 138 VHQDMTAPLSHYFIYTGHNSYLTGNQLSSDCSEVPVIKALQRGVRVIELDLWPNSTGTDI 197

Query: 183 DVLHGRTLTTPVEFVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDM 242
           +VLHGRTLTTPV  ++CLKSI++ AF +SPYPVIITLEDHLTPDLQAK AE   + FG M
Sbjct: 198 NVLHGRTLTTPVPLMKCLKSIRDYAFSSSPYPVIITLEDHLTPDLQAKVAEMATQIFGQM 257

Query: 243 LFFPEAEYLQEFPSPEELKYRIIISTKPPKEYLRSKN--VKGENS----SSEDETDDEDG 302
           L++PE++ L EFPSP  L +RIIISTKPPKEYL S+N  VK +++    SSEDET   + 
Sbjct: 258 LYYPESDSLLEFPSPASLLHRIIISTKPPKEYLESRNPIVKQKDNNVSPSSEDETPRTEE 317

Query: 303 VDTSES--------ESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKA 362
           + T ES          S+SD  D E+       YKRLI IHAGKPKG +KE +KV  DK 
Sbjct: 318 IQTLESMLFDQDFESKSDSDQEDEEASEDQKPAYKRLITIHAGKPKGTVKEEMKVVVDKV 377

Query: 363 RRLSLSEKALKKATKSHGMDVIRFTQKNMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFN 422
           RRLSLSE+ L +   S+  DV+RFTQ+N+LRIYPKGTR NSSNY P+  W HGAQM+AFN
Sbjct: 378 RRLSLSEQELDRTCSSNSQDVVRFTQRNLLRIYPKGTRFNSSNYKPLIGWTHGAQMIAFN 437

Query: 423 MQGYGKFLSLMYGMFRSNGGCGYVKKPDFLL-------TVDPHG--------KVKVYMGV 482
           MQGYGK L LM+GMFR+NGGCGYVKKP+FL+         DP          KVKVYMG 
Sbjct: 438 MQGYGKSLWLMHGMFRANGGCGYVKKPNFLMKKGFHDEVFDPRKKLPVKETLKVKVYMGD 497

Query: 483 GWNLDFESTHFHLYSPPDFYARIGIAGAPDDAIIKRTEIKEETWSPVWNEEFTYPLTIPE 542
           GW +DF  THF  YSPPDFY ++ I G P D   K+T+I E+ W P+W+EEF++PLT+PE
Sbjct: 498 GWRMDFSHTHFDAYSPPDFYTKMFIVGVPADNAKKKTKIIEDNWYPIWDEEFSFPLTVPE 557

Query: 543 LALLRIEVYSYNISDKDVFGGQNCLPVPEVKAGIHAVPLFDRKGSK 545
           LALLRIEV  Y++S+KD FGGQ CLPV E++ GI +VPL+D+KG K
Sbjct: 558 LALLRIEVREYDMSEKDDFGGQTCLPVAELRPGIRSVPLYDKKGEK 600

BLAST of Carg12430 vs. TAIR 10
Match: AT5G58690.1 (phosphatidylinositol-speciwc phospholipase C5 )

HSP 1 Score: 615.5 bits (1586), Expect = 5.1e-176
Identity = 311/563 (55.24%), Postives = 405/563 (71.94%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASM 60
           MG+Y++ LC   + +M+   PP DV  AF++Y +   ++MT EQ   FLVE Q +    +
Sbjct: 5   MGSYKMGLCCSDKLRMNRGAPPQDVVTAFVEY-TEGRSHMTAEQLCRFLVEVQDETEVLV 64

Query: 61  ADAQRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120
           +DA++I+E++   RHH I+K  +H+LNLDDF+ +LFS DLN P+  +VHQDM +PLSHY+
Sbjct: 65  SDAEKIIERITCERHH-ITKFLRHTLNLDDFFSFLFSDDLNHPIDSKVHQDMASPLSHYF 124

Query: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           IYTSHN+YLTGNQ++S+ SDVP+I ALK GVR +ELD+WPNS +DDI VLHG   T PVE
Sbjct: 125 IYTSHNSYLTGNQINSECSDVPLIKALKRGVRALELDMWPNSTKDDILVLHGWAWTPPVE 184

Query: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240
            V+CL+SIKE+AF AS YPVI+TLEDHLTPDLQAKAAE +KE F DM++FPEA  L+EFP
Sbjct: 185 LVKCLRSIKEHAFYASAYPVILTLEDHLTPDLQAKAAEMMKEIFMDMVYFPEAGGLKEFP 244

Query: 241 SPEELKYRIIISTKPPKEYLR----SKNVKGENSSSEDETDDEDGVDTSESESSESDDSD 300
           SPE+LKY+I+ISTKPPK  LR    S++     +SS+   DDE   + +    +E D  D
Sbjct: 245 SPEDLKYKIVISTKPPKGSLRKDKDSESDASGKASSDVSADDEKTEEETSEAKNEEDGFD 304

Query: 301 YESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKATKSHGMDVIR 360
            ES       Y RLI I +G  K GLKEAL +     RRLSL E+  KKAT+ +G +VI+
Sbjct: 305 QESSNLDFLTYSRLITIPSGNAKNGLKEALTIDNGGVRRLSLREQKFKKATEMYGTEVIK 364

Query: 361 FTQKNMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYGMFRSNGGCGY 420
           FTQKN+LRIYPK TRVNSSNY P + W++GAQMVAFNMQGYG+ L +M+GMFR NGGCGY
Sbjct: 365 FTQKNLLRIYPKATRVNSSNYRPYNGWMYGAQMVAFNMQGYGRALWMMHGMFRGNGGCGY 424

Query: 421 VKKPDFLLTVDPHG---------------KVKVYMGVGWNLDFESTHFHLYSPPDFYARI 480
           VKKPDF++  +  G               KVKVYMG GW+  F+ T F+ +S P+FY R+
Sbjct: 425 VKKPDFMMNNNLSGEVFNPKAKLPIKKTLKVKVYMGKGWDSGFQRTCFNTWSSPNFYTRV 484

Query: 481 GIAGAPDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNISDKDVFGGQN 540
           GI G   D ++K+T+ +++TW P WNEEF + LT+PELALLRIEV+ YN+ +KD F GQ 
Sbjct: 485 GITGVRGDKVMKKTKKEQKTWEPFWNEEFEFQLTVPELALLRIEVHDYNMPEKDDFSGQT 544

Query: 541 CLPVPEVKAGIHAVPLFDRKGSK 545
           CLPV E++ GI +VPL+DRKG +
Sbjct: 545 CLPVSELRQGIRSVPLYDRKGER 565

BLAST of Carg12430 vs. TAIR 10
Match: AT3G08510.1 (phospholipase C 2 )

HSP 1 Score: 597.4 bits (1539), Expect = 1.4e-170
Identity = 311/573 (54.28%), Postives = 389/573 (67.89%), Query Frame = 0

Query: 4   YRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASMADA 63
           Y+VC CF RRF+ +  E P ++K  F KY+      MTV+  H FL++ Q    A+  DA
Sbjct: 6   YKVCFCFRRRFRYTASEAPREIKTIFEKYSENGV--MTVDHLHRFLIDVQKQDKATREDA 65

Query: 64  QRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVS-DQVHQDMRAPLSHYYIY 123
           Q I+       +   S L ++ L+LD F+ YLF  D NPP++  +VH DM AP+SHY+I+
Sbjct: 66  QSII-------NSASSLLHRNGLHLDAFFKYLFG-DNNPPLALHKVHHDMDAPISHYFIF 125

Query: 124 TSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEFV 183
           T HN+YLTGNQLSSD S+VPII+ALK GVRV+ELD+WPNS +DDIDVLHG TLTTPV  +
Sbjct: 126 TGHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGLI 185

Query: 184 RCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPE-AEYLQEFPS 243
           +CLK+I+ +AF  S YPV++TLEDHLTPDLQ+K AE + E FG++LF P   E L+EFPS
Sbjct: 186 KCLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFPS 245

Query: 244 PEELKYRIIISTKPPKEYLRSKNV----KGENSSSED-----------ETDDEDGVDTSE 303
           P  LK RIIISTKPPKEY   K+V    KG++   E+               E   D   
Sbjct: 246 PNSLKRRIIISTKPPKEYKEGKDVEVVQKGKDLGDEEVWGREVPSFIQRNKSEAKDDLDG 305

Query: 304 SESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKA 363
           ++  + DD + +S      +YK LIAIHAGKPKGG+ E LKV  DK RRLSLSE+ L+KA
Sbjct: 306 NDDDDDDDDEDKSKINAPPQYKHLIAIHAGKPKGGITECLKVDPDKVRRLSLSEEQLEKA 365

Query: 364 TKSHGMDVIRFTQKNMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYG 423
            + +   ++RFTQ N+LRIYPKGTRV SSNYNP+  W HGAQMVAFNMQGYG+ L LM G
Sbjct: 366 AEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRSLWLMQG 425

Query: 424 MFRSNGGCGYVKKPDFLL-------TVDPHG--------KVKVYMGVGWNLDFESTHFHL 483
           MFR+NGGCGY+KKPD LL         DP          +V VYMG GW  DF  THF  
Sbjct: 426 MFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFRHTHFDQ 485

Query: 484 YSPPDFYARIGIAGAPDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNI 543
           YSPPDFY R+GIAG P D ++K+T+  E+ W P W+E F +PLT+PELALLR+EV+ Y++
Sbjct: 486 YSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLEVHEYDM 545

Query: 544 SDKDVFGGQNCLPVPEVKAGIHAVPLFDRKGSK 545
           S+KD FGGQ CLPV E+  GI A PL  RKG K
Sbjct: 546 SEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEK 568

BLAST of Carg12430 vs. TAIR 10
Match: AT3G08510.2 (phospholipase C 2 )

HSP 1 Score: 597.4 bits (1539), Expect = 1.4e-170
Identity = 311/573 (54.28%), Postives = 389/573 (67.89%), Query Frame = 0

Query: 4   YRVCLCFIRRFKMSTDEPPSDVKEAFLKYASAAAANMTVEQFHSFLVEFQGDQTASMADA 63
           Y+VC CF RRF+ +  E P ++K  F KY+      MTV+  H FL++ Q    A+  DA
Sbjct: 6   YKVCFCFRRRFRYTASEAPREIKTIFEKYSENGV--MTVDHLHRFLIDVQKQDKATREDA 65

Query: 64  QRIVEQVLQRRHHVISKLTKHSLNLDDFYYYLFSMDLNPPVS-DQVHQDMRAPLSHYYIY 123
           Q I+       +   S L ++ L+LD F+ YLF  D NPP++  +VH DM AP+SHY+I+
Sbjct: 66  QSII-------NSASSLLHRNGLHLDAFFKYLFG-DNNPPLALHKVHHDMDAPISHYFIF 125

Query: 124 TSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEFV 183
           T HN+YLTGNQLSSD S+VPII+ALK GVRV+ELD+WPNS +DDIDVLHG TLTTPV  +
Sbjct: 126 TGHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGLI 185

Query: 184 RCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPE-AEYLQEFPS 243
           +CLK+I+ +AF  S YPV++TLEDHLTPDLQ+K AE + E FG++LF P   E L+EFPS
Sbjct: 186 KCLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFPS 245

Query: 244 PEELKYRIIISTKPPKEYLRSKNV----KGENSSSED-----------ETDDEDGVDTSE 303
           P  LK RIIISTKPPKEY   K+V    KG++   E+               E   D   
Sbjct: 246 PNSLKRRIIISTKPPKEYKEGKDVEVVQKGKDLGDEEVWGREVPSFIQRNKSEAKDDLDG 305

Query: 304 SESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKARRLSLSEKALKKA 363
           ++  + DD + +S      +YK LIAIHAGKPKGG+ E LKV  DK RRLSLSE+ L+KA
Sbjct: 306 NDDDDDDDDEDKSKINAPPQYKHLIAIHAGKPKGGITECLKVDPDKVRRLSLSEEQLEKA 365

Query: 364 TKSHGMDVIRFTQKNMLRIYPKGTRVNSSNYNPMSAWLHGAQMVAFNMQGYGKFLSLMYG 423
            + +   ++RFTQ N+LRIYPKGTRV SSNYNP+  W HGAQMVAFNMQGYG+ L LM G
Sbjct: 366 AEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRSLWLMQG 425

Query: 424 MFRSNGGCGYVKKPDFLL-------TVDPHG--------KVKVYMGVGWNLDFESTHFHL 483
           MFR+NGGCGY+KKPD LL         DP          +V VYMG GW  DF  THF  
Sbjct: 426 MFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFRHTHFDQ 485

Query: 484 YSPPDFYARIGIAGAPDDAIIKRTEIKEETWSPVWNEEFTYPLTIPELALLRIEVYSYNI 543
           YSPPDFY R+GIAG P D ++K+T+  E+ W P W+E F +PLT+PELALLR+EV+ Y++
Sbjct: 486 YSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLEVHEYDM 545

Query: 544 SDKDVFGGQNCLPVPEVKAGIHAVPLFDRKGSK 545
           S+KD FGGQ CLPV E+  GI A PL  RKG K
Sbjct: 546 SEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEK 568

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7036987.10.0e+00100.00Phosphoinositide phospholipase C 4, partial [Cucurbita argyrosperma subsp. argyr... [more]
KAG7019134.10.0e+0084.08Phosphoinositide phospholipase C 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022948784.10.0e+0096.28phosphoinositide phospholipase C 4-like [Cucurbita moschata][more]
XP_023523267.10.0e+0096.10phosphoinositide phospholipase C 4-like [Cucurbita pepo subsp. pepo][more]
XP_022998188.10.0e+0095.74phosphoinositide phospholipase C 4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q944C13.2e-18356.53Phosphoinositide phospholipase C 4 OS=Arabidopsis thaliana OX=3702 GN=PLC4 PE=2 ... [more]
Q8GV435.7e-18056.31Phosphoinositide phospholipase C 6 OS=Arabidopsis thaliana OX=3702 GN=PLC6 PE=2 ... [more]
Q944C27.2e-17555.24Phosphoinositide phospholipase C 5 OS=Arabidopsis thaliana OX=3702 GN=PLC5 PE=2 ... [more]
Q390332.0e-16954.28Phosphoinositide phospholipase C 2 OS=Arabidopsis thaliana OX=3702 GN=PLC2 PE=1 ... [more]
Q9LY519.7e-16452.24Phosphoinositide phospholipase C 7 OS=Arabidopsis thaliana OX=3702 GN=PLC7 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1GAY70.0e+0096.28Phosphoinositide phospholipase C OS=Cucurbita moschata OX=3662 GN=LOC111452349 P... [more]
A0A6J1KG310.0e+0095.74Phosphoinositide phospholipase C OS=Cucurbita maxima OX=3661 GN=LOC111492913 PE=... [more]
A0A1S3C6M64.1e-29088.65Phosphoinositide phospholipase C OS=Cucumis melo OX=3656 GN=LOC103497078 PE=4 SV... [more]
A0A6J1I2U59.1e-29088.30Phosphoinositide phospholipase C OS=Cucurbita maxima OX=3661 GN=LOC111469413 PE=... [more]
A0A0A0LVI72.2e-28888.48Phosphoinositide phospholipase C OS=Cucumis sativus OX=3659 GN=Csa_1G531180 PE=4... [more]
Match NameE-valueIdentityDescription
AT5G58700.12.3e-18456.53phosphatidylinositol-speciwc phospholipase C4 [more]
AT2G40116.14.0e-18156.31Phosphoinositide-specific phospholipase C family protein [more]
AT5G58690.15.1e-17655.24phosphatidylinositol-speciwc phospholipase C5 [more]
AT3G08510.11.4e-17054.28phospholipase C 2 [more]
AT3G08510.21.4e-17054.28phospholipase C 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001192Phosphoinositide phospholipase C familyPRINTSPR00390PHPHLIPASECcoord: 362..383
score: 59.78
coord: 383..401
score: 53.72
coord: 523..533
score: 36.54
coord: 115..133
score: 74.68
coord: 141..161
score: 50.49
coord: 238..255
score: 51.99
IPR001192Phosphoinositide phospholipase C familyPANTHERPTHR10336PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEINcoord: 1..545
coord: 544..693
IPR000909Phosphatidylinositol-specific phospholipase C, X domainSMARTSM00148plcx_3coord: 110..254
e-value: 3.2E-72
score: 255.9
IPR000909Phosphatidylinositol-specific phospholipase C, X domainPFAMPF00388PI-PLC-Xcoord: 633..693
e-value: 1.9E-17
score: 63.2
coord: 112..254
e-value: 1.3E-50
score: 170.8
IPR001711Phospholipase C, phosphatidylinositol-specific, Y domainSMARTSM00149plcy_3coord: 307..424
e-value: 1.8E-59
score: 213.6
IPR001711Phospholipase C, phosphatidylinositol-specific, Y domainPFAMPF00387PI-PLC-Ycoord: 335..422
e-value: 2.9E-28
score: 98.5
IPR001711Phospholipase C, phosphatidylinositol-specific, Y domainPROSITEPS50008PIPLC_Y_DOMAINcoord: 337..423
score: 36.216
IPR000008C2 domainSMARTSM00239C2_3ccoord: 429..537
e-value: 7.8E-11
score: 52.0
IPR000008C2 domainPFAMPF00168C2coord: 454..528
e-value: 2.0E-8
score: 34.5
IPR000008C2 domainPROSITEPS50004C2coord: 418..539
score: 15.115917
IPR035892C2 domain superfamilyGENE3D2.60.40.150C2 domaincoord: 432..549
e-value: 4.0E-19
score: 71.0
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 432..545
IPR017946PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamilyGENE3D3.20.20.190Phosphatidylinositol (PI) phosphodiesterasecoord: 108..431
e-value: 8.5E-102
score: 342.5
coord: 628..693
e-value: 3.5E-21
score: 77.8
IPR017946PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamilySUPERFAMILY51695PLC-like phosphodiesterasescoord: 114..433
IPR017946PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamilySUPERFAMILY51695PLC-like phosphodiesterasescoord: 631..693
IPR015359Phosphoinositide-specific phospholipase C, EF-hand-like domainPFAMPF09279EF-hand_likecoord: 23..100
e-value: 3.0E-4
score: 21.2
NoneNo IPR availableGENE3D1.10.238.10coord: 20..107
e-value: 2.5E-11
score: 45.7
coord: 550..626
e-value: 2.0E-8
score: 36.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 261..298
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 272..295
NoneNo IPR availablePANTHERPTHR10336:SF187PHOSPHOINOSITIDE PHOSPHOLIPASE Ccoord: 1..545
coord: 544..693
NoneNo IPR availablePROSITEPS50007PIPLC_X_DOMAINcoord: 631..693
score: 22.1504
NoneNo IPR availablePROSITEPS50007PIPLC_X_DOMAINcoord: 110..254
score: 43.408199
NoneNo IPR availableCDDcd08599PI-PLCc_plantcoord: 109..411
e-value: 5.49114E-120
score: 355.912
NoneNo IPR availableCDDcd00275C2_PLC_likecoord: 431..543
e-value: 1.08605E-25
score: 100.694
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 8..112
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 550..633

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12430-RACarg12430-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0016042 lipid catabolic process
biological_process GO:0048015 phosphatidylinositol-mediated signaling
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006629 lipid metabolic process
biological_process GO:0007165 signal transduction
cellular_component GO:0048046 apoplast
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005773 vacuole
molecular_function GO:0004565 beta-galactosidase activity
molecular_function GO:0004435 phosphatidylinositol phospholipase C activity
molecular_function GO:0008081 phosphoric diester hydrolase activity