Carg12234 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg12234 vs. NCBI nr
Match: KAG7021887.1 (putative RNA-dependent RNA polymerase 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2048.5 bits (5306), Expect = 0.0e+00
Identity = 1018/1018 (100.00%), Postives = 1018/1018 (100.00%), Query Frame = 0

Query: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60
            MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN
Sbjct: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60

Query: 61   LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120
            LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS
Sbjct: 61   LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120

Query: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180
            SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER
Sbjct: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180

Query: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240
            VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY
Sbjct: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240

Query: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300
            RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL
Sbjct: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300

Query: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360
            RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH
Sbjct: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360

Query: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNGNVMYRDGKPLIHTD 420
            PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNGNVMYRDGKPLIHTD
Sbjct: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNGNVMYRDGKPLIHTD 420

Query: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480
            GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF
Sbjct: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480

Query: 481  RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540
            RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA
Sbjct: 481  RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540

Query: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600
            YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI
Sbjct: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600

Query: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660
            LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN
Sbjct: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660

Query: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720
            GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD
Sbjct: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720

Query: 721  FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780
            FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ
Sbjct: 721  FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780

Query: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV 840
            PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV
Sbjct: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV 840

Query: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900
            PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL
Sbjct: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900

Query: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA 960
            MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA
Sbjct: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA 960

Query: 961  LAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1019
            LAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS
Sbjct: 961  LAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1018

BLAST of Carg12234 vs. NCBI nr
Match: KAG6587993.1 (putative RNA-dependent RNA polymerase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2045.4 bits (5298), Expect = 0.0e+00
Identity = 1016/1018 (99.80%), Postives = 1018/1018 (100.00%), Query Frame = 0

Query: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60
            MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGE+MALDALRKISSCSVRN
Sbjct: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEKMALDALRKISSCSVRN 60

Query: 61   LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120
            LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS
Sbjct: 61   LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120

Query: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180
            SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER
Sbjct: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180

Query: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240
            VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY
Sbjct: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240

Query: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300
            RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL
Sbjct: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300

Query: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360
            RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH
Sbjct: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360

Query: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNGNVMYRDGKPLIHTD 420
            PHTVPSVASYMARFSLILSKTIKLKIDLSSVNV+RIADIPCKDVNGNVMYRDGKPLIHTD
Sbjct: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVQRIADIPCKDVNGNVMYRDGKPLIHTD 420

Query: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480
            GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF
Sbjct: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480

Query: 481  RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540
            RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA
Sbjct: 481  RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540

Query: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600
            YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI
Sbjct: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600

Query: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660
            LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN
Sbjct: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660

Query: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720
            GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD
Sbjct: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720

Query: 721  FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780
            FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ
Sbjct: 721  FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780

Query: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV 840
            PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV
Sbjct: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV 840

Query: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900
            PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL
Sbjct: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900

Query: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA 960
            MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA
Sbjct: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA 960

Query: 961  LAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1019
            LAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS
Sbjct: 961  LAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1018

BLAST of Carg12234 vs. NCBI nr
Match: XP_022930237.1 (probable RNA-dependent RNA polymerase 5 isoform X2 [Cucurbita moschata])

HSP 1 Score: 2032.7 bits (5265), Expect = 0.0e+00
Identity = 1011/1018 (99.31%), Postives = 1014/1018 (99.61%), Query Frame = 0

Query: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60
            MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN
Sbjct: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60

Query: 61   LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120
            LSGFIMHMVRNDPCISPQNK VAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS
Sbjct: 61   LSGFIMHMVRNDPCISPQNKTVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120

Query: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180
            SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER
Sbjct: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180

Query: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240
            VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY
Sbjct: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240

Query: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300
            RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL
Sbjct: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300

Query: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360
            RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH
Sbjct: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360

Query: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNGNVMYRDGKPLIHTD 420
            PHTVPSVASYMARFSLILSKTIKLKIDLSSVNV+RIADIPCKDVNGNVMYRDGKPLIHTD
Sbjct: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVQRIADIPCKDVNGNVMYRDGKPLIHTD 420

Query: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480
            GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF
Sbjct: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480

Query: 481  RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540
            RLFYNGLAVKGTFLVNKQ PSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA
Sbjct: 481  RLFYNGLAVKGTFLVNKQLPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540

Query: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600
            YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI
Sbjct: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600

Query: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660
            LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN
Sbjct: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660

Query: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720
            GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD
Sbjct: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720

Query: 721  FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780
            FDGDMYWVSRNSQLLEYFR GEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ
Sbjct: 721  FDGDMYWVSRNSQLLEYFRAGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780

Query: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV 840
            PSYAKSVAADNWVALMDQLL+LGEDCKEERN VRAKILQLINIYYDALDAPKKGGKEIEV
Sbjct: 781  PSYAKSVAADNWVALMDQLLVLGEDCKEERNLVRAKILQLINIYYDALDAPKKGGKEIEV 840

Query: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900
            PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL
Sbjct: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900

Query: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA 960
            MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA
Sbjct: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA 960

Query: 961  LAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1019
            LAIYHVTYEYAMSHGCVR+CSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS
Sbjct: 961  LAIYHVTYEYAMSHGCVRYCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1018

BLAST of Carg12234 vs. NCBI nr
Match: XP_022930229.1 (probable RNA-dependent RNA polymerase 5 isoform X1 [Cucurbita moschata])

HSP 1 Score: 2027.7 bits (5252), Expect = 0.0e+00
Identity = 1011/1020 (99.12%), Postives = 1014/1020 (99.41%), Query Frame = 0

Query: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60
            MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN
Sbjct: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60

Query: 61   LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120
            LSGFIMHMVRNDPCISPQNK VAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS
Sbjct: 61   LSGFIMHMVRNDPCISPQNKTVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120

Query: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180
            SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER
Sbjct: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180

Query: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240
            VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY
Sbjct: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240

Query: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300
            RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL
Sbjct: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300

Query: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360
            RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH
Sbjct: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360

Query: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNGNVMYRDGKPLIHTD 420
            PHTVPSVASYMARFSLILSKTIKLKIDLSSVNV+RIADIPCKDVNGNVMYRDGKPLIHTD
Sbjct: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVQRIADIPCKDVNGNVMYRDGKPLIHTD 420

Query: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480
            GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF
Sbjct: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480

Query: 481  RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540
            RLFYNGLAVKGTFLVNKQ PSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA
Sbjct: 481  RLFYNGLAVKGTFLVNKQLPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540

Query: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600
            YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI
Sbjct: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600

Query: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660
            LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN
Sbjct: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660

Query: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720
            GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD
Sbjct: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720

Query: 721  FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780
            FDGDMYWVSRNSQLLEYFR GEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ
Sbjct: 721  FDGDMYWVSRNSQLLEYFRAGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780

Query: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV 840
            PSYAKSVAADNWVALMDQLL+LGEDCKEERN VRAKILQLINIYYDALDAPKKGGKEIEV
Sbjct: 781  PSYAKSVAADNWVALMDQLLVLGEDCKEERNLVRAKILQLINIYYDALDAPKKGGKEIEV 840

Query: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900
            PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL
Sbjct: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900

Query: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKE--ILYGAEELEGSPRWVEEVYK 960
            MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKE  ILYGAEELEGSPRWVEEVYK
Sbjct: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKELQILYGAEELEGSPRWVEEVYK 960

Query: 961  EALAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1019
            EALAIYHVTYEYAMSHGCVR+CSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS
Sbjct: 961  EALAIYHVTYEYAMSHGCVRYCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1020

BLAST of Carg12234 vs. NCBI nr
Match: XP_023531777.1 (probable RNA-dependent RNA polymerase 5 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2014.2 bits (5217), Expect = 0.0e+00
Identity = 999/1018 (98.13%), Postives = 1009/1018 (99.12%), Query Frame = 0

Query: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60
            MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN
Sbjct: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60

Query: 61   LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120
            LSGFIMHMVRNDPCISPQNKMVAV SPHTSPSSTGCVSQLHSPSTCS SLYQSLSSCRVS
Sbjct: 61   LSGFIMHMVRNDPCISPQNKMVAVFSPHTSPSSTGCVSQLHSPSTCSVSLYQSLSSCRVS 120

Query: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180
            SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER
Sbjct: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180

Query: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240
            VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRR+YVDWDRRKTHIYHCYV LDGSY
Sbjct: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRVYVDWDRRKTHIYHCYVELDGSY 240

Query: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300
            RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL
Sbjct: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300

Query: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360
            RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH
Sbjct: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360

Query: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNGNVMYRDGKPLIHTD 420
            PHTV SVASYMARFSLILSKTI LKIDLSSVNV+RIADIPCKDVNGNVMYRDGKPLIHTD
Sbjct: 361  PHTVSSVASYMARFSLILSKTINLKIDLSSVNVQRIADIPCKDVNGNVMYRDGKPLIHTD 420

Query: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480
            GTGFISEDLALECPMNVFKGQAKHDA LKRIPAFEVFQNKTSQVILPGLELREPPLLIQF
Sbjct: 421  GTGFISEDLALECPMNVFKGQAKHDAGLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480

Query: 481  RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540
            RLFYNGLAVKGTFLVNKQ PSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA
Sbjct: 481  RLFYNGLAVKGTFLVNKQLPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540

Query: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600
            YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI
Sbjct: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600

Query: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660
            LSGIPLNESYLQYRLSMLLREEKKSL+SGRLHVPDCYYLMGTADPTCTLESNEVCVILEN
Sbjct: 601  LSGIPLNESYLQYRLSMLLREEKKSLKSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660

Query: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720
            GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD
Sbjct: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720

Query: 721  FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780
            FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIA+IKP+EFSAEDLE ELFKLFLTARFQ
Sbjct: 721  FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIADIKPNEFSAEDLEAELFKLFLTARFQ 780

Query: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV 840
            PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKIL+LIN+YYDALDAPKKGGKEIEV
Sbjct: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILRLINMYYDALDAPKKGGKEIEV 840

Query: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900
            PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL
Sbjct: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900

Query: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA 960
            MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEEL+GSPRW+EEVYKEA
Sbjct: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELDGSPRWMEEVYKEA 960

Query: 961  LAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1019
            LAIYHVTYEYAMSHGCVR+CSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQI CSS
Sbjct: 961  LAIYHVTYEYAMSHGCVRYCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQILCSS 1018

BLAST of Carg12234 vs. ExPASy Swiss-Prot
Match: O82188 (Probable RNA-dependent RNA polymerase 5 OS=Arabidopsis thaliana OX=3702 GN=RDR5 PE=2 SV=2)

HSP 1 Score: 879.4 bits (2271), Expect = 3.9e-254
Identity = 481/1025 (46.93%), Postives = 668/1025 (65.17%), Query Frame = 0

Query: 8    VPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCS---VRNLSGF 67
            + L   +E  L  I  + N  P + + R+ L+S+ E +  + +RK+ S     + NL  F
Sbjct: 10   IALSGRIETALENIYRKHNLTPINDETRQRLSSIPENLGFELVRKVFSLQAGLIYNLDSF 69

Query: 68   IMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVSSPQG 127
            I         +S  N+ V         S TG     +S S     + + L          
Sbjct: 70   I---------VSKVNQAV---------SFTGYPQPANSLSPSGRHVSRVLQ--------- 129

Query: 128  TAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLERVTTA 187
              E+   ++  P   ++ S      + IPQ VALGELEF++ FL+LSYI G+ + +V TA
Sbjct: 130  --EEMSVDSDAPSPKSLKSEDKGGSLHIPQLVALGELEFKKVFLLLSYIPGQHVGQVITA 189

Query: 188  DQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSYRFK- 247
            D+I+    L M ++E+ VW   G  Y  ++DRRM + WD  KTH Y C VA +GSY FK 
Sbjct: 190  DEIRLWKDLPMVEYEAAVWDRLGRHYCPQKDRRM-LQWDSGKTHYYQCNVAPNGSYTFKV 249

Query: 248  -----GPYLNHTKTHLQRVLGDDNVLMVKFA--EDVSDRCSISHPGGSFYAYKKIARDGI 307
                 GP L HT THL +VLGDDNVL VKFA  +  S   SI H    ++ YK IA++GI
Sbjct: 250  LSALQGPLLEHTGTHLHKVLGDDNVLTVKFADVQKSSSTYSIDH----YFTYKGIAKNGI 309

Query: 308  LLGLRRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARS 367
            ++GLRRYQFFVFKDGGKEEKKK+ +T  VKCYF+R +S A++D   PY L+ ++I+EAR 
Sbjct: 310  MIGLRRYQFFVFKDGGKEEKKKDLSTKKVKCYFIRTDSTAFYDMQNPYILTGKSIYEARM 369

Query: 368  LFMHPHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNG-NVMYRDGKP 427
             FMH H  P++A+YMARFSLILSKT  L++D++ +   +I DI C D +G +V+ ++ KP
Sbjct: 370  HFMHVHRAPTLANYMARFSLILSKTKTLEVDMTGITFDQIDDIHCHDQDGKDVLDKNKKP 429

Query: 428  LIHTDGTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPP 487
             IH+DGTG+ISEDLA  CP+N+FKG+      ++                      ++PP
Sbjct: 430  CIHSDGTGYISEDLARMCPLNIFKGKCLRSESIQEAC------------------YQDPP 489

Query: 488  LLIQFRLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSN 547
            LLIQFR+FY+G AVKGTFL+NK+   RT+Q+R SMIKV KDP+L N  T N+LEVV TSN
Sbjct: 490  LLIQFRMFYDGYAVKGTFLLNKKLCPRTVQVRPSMIKVSKDPSLSNFSTFNALEVVTTSN 549

Query: 548  PPKRAYISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFL 607
            PPKR  +S+NL+ALL+YGG+P E+F++IL++ L + + +F +KRAAL  ++N G+MDD  
Sbjct: 550  PPKRTKLSKNLVALLSYGGIPNEFFLDILLNTLEESKSIFYNKRAALNAALNYGEMDDQN 609

Query: 608  VARMILSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVC 667
             A+MIL GIPL+E +L+  LS+LL+ EK  L++G+L V + YYLMGT DPT  L+ +EVC
Sbjct: 610  AAQMILVGIPLDEPHLKNYLSILLKTEKNDLKAGKLPVTESYYLMGTVDPTGALKEDEVC 669

Query: 668  VILENGQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADE 727
            VILE+GQI+G+VLVYRNPGLHFGDIH+L A YV+ L   VGN+K+ +FF  KGPRS+ DE
Sbjct: 670  VILESGQISGEVLVYRNPGLHFGDIHILKATYVKALEEYVGNSKFAVFFPQKGPRSLGDE 729

Query: 728  IAGGDFDGDMYWVSRNSQLLEYFRPGEPW-RPSPSMEVIANIKPSEFSAEDLEEELFKLF 787
            IAGGDFDGDMY++SRN +LLE F+P EPW   +P  +  +   PS+ S E+LEEELF++F
Sbjct: 730  IAGGDFDGDMYFISRNPELLENFKPSEPWVSLTPPSKSNSGRAPSQLSPEELEEELFEMF 789

Query: 788  LTARFQPSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKG 847
            LTA F  S    +AAD+W+ +MD+ L+LG+D  EE+  ++ K+L+LI+IYYDALDAPKKG
Sbjct: 790  LTAGFHASNVIGIAADSWLTIMDRFLILGDDRAEEKAEMKKKMLELIDIYYDALDAPKKG 849

Query: 848  GKEIEVPEHLKAGTFPHFMERGKKSYTSTSILGQIFDTV--NMFQEEAPNIEVQKLPCFE 907
             K + +P  LK   FPH+MER KK + STSILG IFD V     +E +P+ E+ KLPCFE
Sbjct: 850  DK-VYLPNKLKPDIFPHYMERDKK-FQSTSILGLIFDFVKSQTTEEPSPSSEISKLPCFE 909

Query: 908  EE-VADDTLMKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPR 967
            +E V++  + K +  Y+ YR EM  A++ D   K+ +A   I++YK+  YGA   E S +
Sbjct: 910  DEPVSEFHMQKCRLWYDNYRTEMTQAMKTD---KDESANEVIQRYKQEFYGAAGFEDSKK 969

Query: 968  WVEEVYKEALAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNL-CARKHSENSFLCLPSV 1016
             +EE+Y +ALA+Y + Y+YA+ H  V  C F W+VAG  L      +K  E   +C PSV
Sbjct: 970  SLEELYPQALALYKIVYDYAI-HAGVSKCRFVWKVAGPVLCRFYLNKKMQEKCLVCAPSV 976

BLAST of Carg12234 vs. ExPASy Swiss-Prot
Match: O82190 (Probable RNA-dependent RNA polymerase 3 OS=Arabidopsis thaliana OX=3702 GN=RDR3 PE=3 SV=2)

HSP 1 Score: 862.8 bits (2228), Expect = 3.8e-249
Identity = 482/1017 (47.39%), Postives = 657/1017 (64.60%), Query Frame = 0

Query: 7    EVPLPPSVEQFLLQICTQQN-QPPPDAKARRALASLGEEMALDALRKI-SSCSVRNLSGF 66
            E+ L  SVE  L +I  + N + P   + RR L+S+ EE+AL+ LRK+ +   ++ L G 
Sbjct: 14   EIALLGSVETMLEKIYEKHNHRLPISVETRRKLSSISEELALETLRKVFNKPYLKTLDGL 73

Query: 67   IMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVSSPQG 126
            IM+ V+    +          SP  SP      S + SP T       +  SCR S    
Sbjct: 74   IMYFVKGTVTVDG--------SPRLSPGE----SPVQSPRT------PAKKSCRAS---- 133

Query: 127  TAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESL-ERVTT 186
              +    +  TP    +          IP  +ALGELEF++AFL+LSYIGGESL E V +
Sbjct: 134  --QDVSLDLETPSPKFMKREENGGSKYIPPLLALGELEFKKAFLLLSYIGGESLVEEVIS 193

Query: 187  ADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSYRFK 246
             DQI+    L M  +E+ VW   G++Y + ++RR  ++ D   TH Y C+VA DGSY+FK
Sbjct: 194  GDQIRKWKDLPMVSYEAAVWNRLGQRYCSPKERRRPLEGDSGMTHYYQCHVATDGSYKFK 253

Query: 247  GPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGLRRY 306
            G  L +T THL +VLGDDNVL VKF + +      ++    +  YK IA++GI++GLRRY
Sbjct: 254  GHLLENTGTHLHKVLGDDNVLTVKFDKVLGVE---TYCNDLYSTYKGIAKNGIMVGLRRY 313

Query: 307  QFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMHPHT 366
            +FFVFKDGGKEEKKK+ +T  VKCYF+R +S A  D   PY  + +++ EAR  FMH +T
Sbjct: 314  RFFVFKDGGKEEKKKDVSTKGVKCYFIRTDSTASIDMQNPYIFAGKSMHEARMHFMHVNT 373

Query: 367  VPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNG-NVMYRDGKPLIHTDGT 426
            + S+ +YM+RFSLILSKT  L++D++ +   +I DI C D +  +V+ ++GKP IH+DGT
Sbjct: 374  LSSLPNYMSRFSLILSKTKTLEVDMTGITFEQIDDIHCHDQDDKDVLDKNGKPCIHSDGT 433

Query: 427  GFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQFRL 486
            G+ISEDLA  CP+N+FKG++    ++         Q+K       G   +EPPLLIQFR+
Sbjct: 434  GYISEDLARMCPVNIFKGKSMRSNNI---------QSKNLNFEGQGPCGQEPPLLIQFRI 493

Query: 487  FYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRAYI 546
            FYNG AVKGTFL NK+ P RT+Q+R SMIKV +D  L N  T NSLEVV TSNPP++A +
Sbjct: 494  FYNGYAVKGTFLTNKKLPPRTVQVRPSMIKVYEDRTLSNLSTFNSLEVVTTSNPPRKARL 553

Query: 547  SRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMILS 606
            SRNL+ALL+YGGVP ++F+NIL + L + + +F S+RAA + +IN G  DD   A MIL 
Sbjct: 554  SRNLVALLSYGGVPNDFFLNILRNTLEESKTIFYSERAAFKAAINYG--DDQYTADMILV 613

Query: 607  GIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILENGQ 666
            GIPL+E YL+ RLS LL+ E+ +L++GR  + + YY+MGT DPT  L+ NE+CVIL +GQ
Sbjct: 614  GIPLDEPYLKDRLSYLLKTERNALKAGRFPIDESYYIMGTVDPTGELKENEICVILHSGQ 673

Query: 667  INGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGDFD 726
            I+G VLVYRNPGLHFGDIHVL A YV+ L   VGNAK+ +FF  KGPRS+ DEIAGGDFD
Sbjct: 674  ISGDVLVYRNPGLHFGDIHVLKATYVKALEDYVGNAKFAVFFPQKGPRSLGDEIAGGDFD 733

Query: 727  GDMYWVSRNSQLLEYFRPGEPW-RPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQP 786
            GDMY++SRN +LLE+F+P EPW   S   ++    KPSE S E+LEEELFK+FL ARF  
Sbjct: 734  GDMYFISRNPKLLEHFKPSEPWVSSSKPSKIYCGRKPSELSEEELEEELFKMFLKARFCK 793

Query: 787  SYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEVP 846
                 +AAD W+ +MD  L LG++  +E+   +  IL+LI+IYYDALDAPKKG K +++P
Sbjct: 794  RDVIGMAADCWLGIMDPFLTLGDESAKEKYERKKNILKLIDIYYDALDAPKKGAK-VDLP 853

Query: 847  EHLKAGTFPHFMERG-KKSYTSTSILGQIFDTVNMFQ-EEAPNIEVQKLPCFEEE-VADD 906
              L+   FPH+MER  K+ + STSILG IFDTV+    EE P  E+ KL  FE+E V   
Sbjct: 854  PDLEIKNFPHYMERDPKRDFRSTSILGLIFDTVDSHNAEEPPPSEISKLWYFEDEPVPKS 913

Query: 907  TLMKWKFQYEMYRKEMRDA-LQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVY 966
             + K+   YE YR EM  A ++ D   +N      I++YK+  YGA   E S + +EE+Y
Sbjct: 914  HMDKFTSWYENYRSEMSQAMMETDKVKRNQLTNEVIQRYKQDFYGAAGFEDSNKSLEELY 973

Query: 967  KEALAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQI 1015
             +ALA+Y+V Y+YA+  G V  C+FAW VAG  L     +K  + S +   SV++++
Sbjct: 974  PQALALYNVVYDYAIQEG-VAKCTFAWNVAGPVLCKFYLKKTKDKSVVASTSVLKKL 990

BLAST of Carg12234 vs. ExPASy Swiss-Prot
Match: O82189 (Probable RNA-dependent RNA polymerase 4 OS=Arabidopsis thaliana OX=3702 GN=RDR4 PE=3 SV=2)

HSP 1 Score: 797.3 bits (2058), Expect = 2.0e-229
Identity = 442/967 (45.71%), Postives = 618/967 (63.91%), Query Frame = 0

Query: 7   EVPLPPSVEQFLLQICTQQNQPPP-DAKARRALASLGEEMALDALRKISSC-SVRNLSGF 66
           E+ L  SVE  L ++  + N  PP   + RR L+S+ EE+AL+ LRK+ +  +V+ L G 
Sbjct: 19  EIALLGSVETMLEKVYGKHNHHPPIKVETRRRLSSISEELALETLRKVLNMPNVKTLDGI 78

Query: 67  IMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVSSPQG 126
           I++ + +   +    ++ +  SP   P + G                    SCR S  + 
Sbjct: 79  IIYFLNDAVTVDGSPRLWSGESPVQFPRTPG------------------KKSCRASQAE- 138

Query: 127 TAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLERVTTA 186
                  +   P    +      +   I   +ALGELEF++AFL+L+Y+GG+SL  V + 
Sbjct: 139 ----VSLDREDPSPKFLRGDENGESKHISLLLALGELEFKKAFLLLTYLGGKSLGEVISG 198

Query: 187 DQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSYRFKG 246
           D+I+    L M  +E  VW   G+      + RM ++ D  KTH Y C+VA DGSYR KG
Sbjct: 199 DEIRQWKDLPMVAYERAVWFKLGQ-----NEERMQLESDSGKTHYYQCHVAPDGSYRLKG 258

Query: 247 PYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGLRRYQ 306
            +L +T THL +VLGDDNVL V+F +   +     +P   +  YK+IA++GI++GLRRYQ
Sbjct: 259 YFLENTGTHLHKVLGDDNVLTVRFDQLPKESTYCDNP---YSKYKEIAKNGIMVGLRRYQ 318

Query: 307 FFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMHPHTV 366
           FFVFKDGGK EKKK  +T  VKCYF+R  S A  D   PY LS  +I EAR  FMH HT+
Sbjct: 319 FFVFKDGGKAEKKKRNSTKQVKCYFIRTGSTASSDMENPYILSGMSIHEARMHFMHVHTL 378

Query: 367 PSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKD-VNGNVMYRDGKPLIHTDGTG 426
           PS A+YMARFSLILSKT KL++D++ + V +I DI C D  N +V+ ++GKP IH+DGTG
Sbjct: 379 PSPANYMARFSLILSKTKKLEVDMTEITVMQIDDIHCHDQSNNDVLDKNGKPRIHSDGTG 438

Query: 427 FISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQFRLF 486
           +ISEDLA  CP+N+FKG++    +++                  G  ++EPPLLIQ R+F
Sbjct: 439 YISEDLARMCPLNIFKGKSMRSNNIQ------------------GTCVQEPPLLIQIRMF 498

Query: 487 YNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRAYIS 546
            +G AVKG FL+NK  P +T+Q+R SMIKV KD NL N  T NSLEVV TSNPPKRA +S
Sbjct: 499 NDGSAVKGIFLLNKNLPPQTVQVRPSMIKVYKDKNLSNFSTFNSLEVVTTSNPPKRAKLS 558

Query: 547 RNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMILSG 606
           +NL+ALL+YGGVP ++F++IL++ L   + +F   RAA + +++ G+MDD    +MI++G
Sbjct: 559 KNLVALLSYGGVPNDFFLDILLNTLEKKKTIFFKVRAAGKAALHYGNMDDKNALQMIMAG 618

Query: 607 IPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILENGQI 666
           IPL+E YL++ LS LL+ EK  L++G+L + + YYLMGT DPT  L+ +EV  + ++   
Sbjct: 619 IPLDEPYLKHYLSKLLKLEKDDLKAGKLPIDESYYLMGTVDPTGELKEDEVSGLAKSQD- 678

Query: 667 NGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGDFDG 726
              VLVYRNPGLHFGDIH+L A YV+ L   VGN+KYG+FF  KGPRS+ DEIAGGDFDG
Sbjct: 679 ---VLVYRNPGLHFGDIHILKATYVKSLEQYVGNSKYGVFFPQKGPRSLGDEIAGGDFDG 738

Query: 727 DMYWVSRNSQLLEYFRPGEPW-RPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQPS 786
           DMY++SRN +LLE+++P EPW   SP  ++    +PSE S E LEEELFK+FL   F PS
Sbjct: 739 DMYFISRNPKLLEHYKPSEPWVSSSPRSKIYTGRQPSELSPEQLEEELFKIFLKTGFSPS 798

Query: 787 YAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEVPE 846
                AAD+W+A+MD+ L LG++  +E+  ++ K+L+L +IYYDA+DAPK  G E+ +P 
Sbjct: 799 SVIGQAADSWLAIMDRFLTLGDENVKEKAEMKKKMLKLTDIYYDAIDAPKT-GTEVNLPL 858

Query: 847 HLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQ-EEAPNIEVQKLPCFEEE-VADDTL 906
            +K   FPH+MER  K++ STSILG IFDTV+    E+     + KL CFE+E V++  +
Sbjct: 859 DVKVDLFPHYMER-NKTFKSTSILGLIFDTVDFHNAEDTTPSGISKLQCFEDEPVSEFDM 918

Query: 907 MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA 966
            K K  ++ YRKEM  A+  D D    +    I+KYK+  Y A   + S + +EE+Y +A
Sbjct: 919 EKCKLWHKDYRKEMCQAMNSDDDD---SCNEVIQKYKQEFYSAAGFKESKKILEELYPKA 927

Query: 967 LAIYHVT 968
           LA+Y+VT
Sbjct: 979 LALYNVT 927

BLAST of Carg12234 vs. ExPASy Swiss-Prot
Match: Q5QMN5 (Probable RNA-dependent RNA polymerase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR3 PE=2 SV=2)

HSP 1 Score: 751.1 bits (1938), Expect = 1.6e-215
Identity = 415/901 (46.06%), Postives = 549/901 (60.93%), Query Frame = 0

Query: 153  PQFVALGELEFRRAFLILSYIGGESLERVTTADQIQSLSQLAMEKFESEVWKAFGEKYVN 212
            PQ +AL EL FR+ F++ +Y+  E +E V + D I+SL  L+M +FES++W+ FG KY+ 
Sbjct: 290  PQMLALEELGFRKIFMVFAYLASEKIENVLSVDYIRSLKFLSMAQFESQIWRTFGHKYIA 349

Query: 213  KEDRRMYVDWDRRKTHIYHCYVALDGS---YRFKGPYLNHTKTHLQRVLGDDNVLMVKFA 272
              DR   +D D   T +YHC VA+ G      FKGPY+ +T+THLQ+V+GDDNVL+VKF 
Sbjct: 350  ASDRAKNLDSDPGMTKVYHCNVAIRGDTVVKIFKGPYIENTRTHLQKVVGDDNVLVVKFM 409

Query: 273  EDVSDRCSISHPGGSFYA--YKKIARDGILLGLRRYQFFVFKDGGKEEK-------KKNP 332
              +SD    +    S Y   Y K+A DGI+LGLRRY+FFV+KDGGKEEK        KN 
Sbjct: 410  GKLSD----TKTDFSTYCEHYHKVAEDGIVLGLRRYRFFVYKDGGKEEKLKQEKIEDKNK 469

Query: 333  TTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMHPHTVPSVASYMARFSLILSK 392
             TS V CYFVR ES    D  +PY LS RT+ +AR LFMH  + P++A YMARF+LILSK
Sbjct: 470  CTSPVMCYFVRTESGWNMD--EPYILSGRTVGQARELFMHISSAPTLAKYMARFALILSK 529

Query: 393  TIKLKIDLSSVNVRRIADIPCKDVNGNVMYRDGKPLIHTDGTGFISEDLALECPMNVFKG 452
            TI    DLS+V VRRI D PC D +GNV+++D +PLIHTDGTG +S DLAL CP ++FKG
Sbjct: 530  TITWDADLSAVYVRRIKDEPCMDRHGNVVHKDQEPLIHTDGTGLVSVDLALNCPTSIFKG 589

Query: 453  QAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQFRLFYNGLAVKGTFLVNKQFP 512
            +                             L+  PLL+QFRLFYNG AVKGT LV+++ P
Sbjct: 590  KF----------------------------LKPQPLLMQFRLFYNGSAVKGTVLVDRRLP 649

Query: 513  SRTIQIRNSMIKVEKDP---------------------NLENAETENSLEVVGTSNPPKR 572
              TI IR SM+K+E  P                     +L   ++ NS E+V TSN P+R
Sbjct: 650  PATILIRPSMVKIETHPELSGVRSVNSSEIVSARNAKKSLSGVQSVNSFEIVSTSNRPRR 709

Query: 573  AYISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARM 632
               SR LI LL YGGVP EYF+ +L  A+   +        ALR++ +  DM+D + ARM
Sbjct: 710  TLTSRFLITLLCYGGVPEEYFLELLQSAIEGAENACYDYEDALRIAFSYADMEDSMSARM 769

Query: 633  ILSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILE 692
            ILSGIPL ESYLQ+RL  + ++E+K ++ G++ + +CYYLM T DPT TL  NEVCVILE
Sbjct: 770  ILSGIPLEESYLQHRLDFMAQQERKGIKQGKIPIDECYYLMDTTDPTGTLRPNEVCVILE 829

Query: 693  NGQINGKVLVYRNPGLHFGDIHVLTAKYVEDL-VPVVGNAKYGIFFSCKGPRSVADEIAG 752
            NGQ +G VLVY++PGLHFGDIHVL A Y+ DL    VG AKY I F   GPRS+ADE+A 
Sbjct: 830  NGQFSGDVLVYKHPGLHFGDIHVLKATYIRDLEKEYVGYAKYAILFPISGPRSLADEMAN 889

Query: 753  GDFDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTAR 812
             DFDGD+YWVS+N +LLE+F+P EPW  +   +     KP + +   LE  LF  FL  R
Sbjct: 890  SDFDGDIYWVSKNPKLLEHFKPSEPWVQAIKPKKTKQKKPQDCNESKLERLLFHEFLKTR 949

Query: 813  FQPSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEI 872
            F PS+A   AAD+W+A MD+LL    D + E+  +  K+L+L+++YY ALDAPK G K +
Sbjct: 950  FTPSFALGTAADSWLAYMDRLLTDSLD-EIEKKLIEEKMLKLVDLYYLALDAPKTGNK-V 1009

Query: 873  EVPEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQE----EAPNIEVQKLPCF-EE 932
             +P  L    +PHFM R   SY S+SILGQI+D     +         I V  LPCF E 
Sbjct: 1010 NIPSDLMVKQYPHFMGR-SFSYHSSSILGQIYDKAEDVESLRSCNVQPIGVSLLPCFMER 1069

Query: 933  EVADDTLMKWKFQYEMYRKE--MRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPR 992
            E        W+ +YE Y  +  M     +D + +N+  +   +KYK +LY A E E + R
Sbjct: 1070 EAPPAARHLWQHRYEEYLTDSTMLYRAMVDKEERNMKFQELYEKYKHMLYDASEFEQTQR 1129

Query: 993  WVEEVYKEALAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVM 1013
              ++V+ EA  IY + YE A        C FAW+VAG AL +  A K+  ++ LC   ++
Sbjct: 1130 DPDDVFSEACVIYQIVYEKARWSNDASRCGFAWKVAGRALCHFYALKNEGDTALCSLPLL 1153

BLAST of Carg12234 vs. ExPASy Swiss-Prot
Match: Q5QMN4 (Probable RNA-dependent RNA polymerase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR4 PE=2 SV=2)

HSP 1 Score: 731.5 bits (1887), Expect = 1.3e-209
Identity = 411/935 (43.96%), Postives = 557/935 (59.57%), Query Frame = 0

Query: 133  ASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIG--------------GESL 192
            A  PD + +   +VS     PQ +ALGELEFR+ F+I +Y+                  +
Sbjct: 262  AICPDTTNVLREVVS-----PQMLALGELEFRKIFMIFAYLSWNKKGVKPPLSTPKSSKI 321

Query: 193  ERVTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDG 252
            E V + D I+SL  ++M +FES +W  FG   ++  DR   +D     + +YHC V + G
Sbjct: 322  EDVLSVDSIRSLKSMSMAQFESRIWSTFGHDNISSSDRAKNLDSGPGMSKVYHCNVEIRG 381

Query: 253  S---YRFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYA--YKKIAR 312
                  FKGPY+ + +THLQ+VLGDDNVL+VKF E  SD    +    S Y   Y K+A 
Sbjct: 382  GTVVKIFKGPYIENRRTHLQKVLGDDNVLVVKFMEISSD----TETDLSTYLEHYHKVAE 441

Query: 313  DGILLGLRRYQFFVFKDGGKEEKKKNPT--------TSAVKCYFVRMESAAYFDKLQPYK 372
            +GI+LGLR Y+FF++KDGGKE K K           TS+V+CYFVR ES    D  +PY 
Sbjct: 442  EGIVLGLRCYRFFLYKDGGKENKMKEENREEENKKCTSSVRCYFVRTESGWNMD--EPYI 501

Query: 373  LSNRTIFEARSLFMHPHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVN 432
            LS RTI +AR LFMH  TV ++A YM RF+LI+SKTI L +DLS V V+ I D PC D +
Sbjct: 502  LSGRTIGQARDLFMHIRTVLTLAKYMLRFALIVSKTITLDVDLSEVLVKLIDDEPCLDEH 561

Query: 433  GNVMYRDGKPLIHTDGTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVI 492
            G  ++RDG+ LIHTDGTG ISEDLA +C  +  KG+     D+    A ++  +  +   
Sbjct: 562  GKEVFRDGERLIHTDGTGLISEDLAQKCTYSNSKGKLLEPQDIVDCAASKLMGSNNT--- 621

Query: 493  LPGLELREPPLLIQFRLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETE 552
                   E PLLIQ RLFYNG AVKGT LV+K+ P RTI IR SM+K++  P L   ++ 
Sbjct: 622  ------TEYPLLIQLRLFYNGSAVKGTVLVDKRLPPRTIHIRPSMLKIKTYPELSGVQSV 681

Query: 553  NSL---------------------EVVGTSNPPKRAYISRNLIALLNYGGVPREYFMNIL 612
            NSL                     E+V TSN   R + S NLIALL+YGGVP E+FM +L
Sbjct: 682  NSLDIVSARNAKKSLSGVQSVNSFEIVSTSNRSGRTFTSNNLIALLHYGGVPEEFFMELL 741

Query: 613  VDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMILSGIPLNESYLQYRLSMLLREEKK 672
              A+ +          AL ++ N  DM+D + ARMILSGIPL ESYLQ RL  L   E+K
Sbjct: 742  QTAIEEADNARFDYAGALNIAFNYADMEDSMPARMILSGIPLEESYLQSRLDFLSLLERK 801

Query: 673  SLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILENGQINGKVLVYRNPGLHFGDIHVLT 732
             +++G++ + DCYYLMGTADPT  L  NEVCVIL+ GQ++G VLVY+ PGLH GDIHVL 
Sbjct: 802  GIKNGKIPIDDCYYLMGTADPTGKLGPNEVCVILDYGQVSGDVLVYKYPGLHPGDIHVLK 861

Query: 733  AKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFRPGEPW 792
            A Y  D+  VVGN+K+ I F   G RS+ADE+A  DFDGD+YWVS N +LLE+F+P +PW
Sbjct: 862  ATYSSDIEKVVGNSKHAILFPTTGQRSLADEMANSDFDGDIYWVSLNPKLLEHFKPSKPW 921

Query: 793  RPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQPSYAKSVAADNWVALMDQLLMLGE 852
             P+ +        P +F+  +LE  LF  FL  RF PSYA++ AA NW+  MD+LL +  
Sbjct: 922  VPAITPNGTKQKGPEDFNESELERVLFHEFLKTRFAPSYARATAATNWLVYMDRLLTVSL 981

Query: 853  DCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEVPEHLKAGTFPHFMERGKKSYTSTS 912
            D + E+  +  K+L+L+++YY ALDAPK G K + +P  L    +PHFM+R   SY S+S
Sbjct: 982  D-ESEKKLIEKKMLKLVDLYYLALDAPKMGNK-VNIPRDLMVKQYPHFMDR-SPSYHSSS 1041

Query: 913  ILGQIFDTVN----MFQEEAPNIEVQKLPCF-EEEVADDTLMKWKFQYEMYRKE--MRDA 972
            ILG+I+D       +  +      +  LPCF E +V       W+ +Y  Y  +  +  A
Sbjct: 1042 ILGKIYDKAGDPKPLRSDNVQPTSISSLPCFAERDVPPAIKQLWQHRYNEYLADSSLLYA 1101

Query: 973  LQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEALAIYHVTYEYAMSHGCV 1013
             + D + K +  +   +KYK +LYGA E E +PR +++V+ EA AIY + YE A S   V
Sbjct: 1102 EEADEEEKKIKFQELYEKYKHLLYGASEFEETPRDLDDVFSEACAIYQIAYEKARSANNV 1161

BLAST of Carg12234 vs. ExPASy TrEMBL
Match: A0A6J1EPU3 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111436744 PE=3 SV=1)

HSP 1 Score: 2032.7 bits (5265), Expect = 0.0e+00
Identity = 1011/1018 (99.31%), Postives = 1014/1018 (99.61%), Query Frame = 0

Query: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60
            MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN
Sbjct: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60

Query: 61   LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120
            LSGFIMHMVRNDPCISPQNK VAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS
Sbjct: 61   LSGFIMHMVRNDPCISPQNKTVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120

Query: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180
            SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER
Sbjct: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180

Query: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240
            VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY
Sbjct: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240

Query: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300
            RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL
Sbjct: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300

Query: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360
            RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH
Sbjct: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360

Query: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNGNVMYRDGKPLIHTD 420
            PHTVPSVASYMARFSLILSKTIKLKIDLSSVNV+RIADIPCKDVNGNVMYRDGKPLIHTD
Sbjct: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVQRIADIPCKDVNGNVMYRDGKPLIHTD 420

Query: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480
            GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF
Sbjct: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480

Query: 481  RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540
            RLFYNGLAVKGTFLVNKQ PSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA
Sbjct: 481  RLFYNGLAVKGTFLVNKQLPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540

Query: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600
            YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI
Sbjct: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600

Query: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660
            LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN
Sbjct: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660

Query: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720
            GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD
Sbjct: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720

Query: 721  FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780
            FDGDMYWVSRNSQLLEYFR GEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ
Sbjct: 721  FDGDMYWVSRNSQLLEYFRAGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780

Query: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV 840
            PSYAKSVAADNWVALMDQLL+LGEDCKEERN VRAKILQLINIYYDALDAPKKGGKEIEV
Sbjct: 781  PSYAKSVAADNWVALMDQLLVLGEDCKEERNLVRAKILQLINIYYDALDAPKKGGKEIEV 840

Query: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900
            PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL
Sbjct: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900

Query: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA 960
            MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA
Sbjct: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA 960

Query: 961  LAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1019
            LAIYHVTYEYAMSHGCVR+CSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS
Sbjct: 961  LAIYHVTYEYAMSHGCVRYCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1018

BLAST of Carg12234 vs. ExPASy TrEMBL
Match: A0A6J1EQY7 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111436744 PE=3 SV=1)

HSP 1 Score: 2027.7 bits (5252), Expect = 0.0e+00
Identity = 1011/1020 (99.12%), Postives = 1014/1020 (99.41%), Query Frame = 0

Query: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60
            MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN
Sbjct: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60

Query: 61   LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120
            LSGFIMHMVRNDPCISPQNK VAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS
Sbjct: 61   LSGFIMHMVRNDPCISPQNKTVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120

Query: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180
            SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER
Sbjct: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180

Query: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240
            VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY
Sbjct: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240

Query: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300
            RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL
Sbjct: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300

Query: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360
            RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH
Sbjct: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360

Query: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNGNVMYRDGKPLIHTD 420
            PHTVPSVASYMARFSLILSKTIKLKIDLSSVNV+RIADIPCKDVNGNVMYRDGKPLIHTD
Sbjct: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVQRIADIPCKDVNGNVMYRDGKPLIHTD 420

Query: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480
            GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF
Sbjct: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480

Query: 481  RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540
            RLFYNGLAVKGTFLVNKQ PSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA
Sbjct: 481  RLFYNGLAVKGTFLVNKQLPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540

Query: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600
            YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI
Sbjct: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600

Query: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660
            LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN
Sbjct: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660

Query: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720
            GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD
Sbjct: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720

Query: 721  FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780
            FDGDMYWVSRNSQLLEYFR GEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ
Sbjct: 721  FDGDMYWVSRNSQLLEYFRAGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780

Query: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV 840
            PSYAKSVAADNWVALMDQLL+LGEDCKEERN VRAKILQLINIYYDALDAPKKGGKEIEV
Sbjct: 781  PSYAKSVAADNWVALMDQLLVLGEDCKEERNLVRAKILQLINIYYDALDAPKKGGKEIEV 840

Query: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900
            PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL
Sbjct: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900

Query: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKE--ILYGAEELEGSPRWVEEVYK 960
            MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKE  ILYGAEELEGSPRWVEEVYK
Sbjct: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKELQILYGAEELEGSPRWVEEVYK 960

Query: 961  EALAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1019
            EALAIYHVTYEYAMSHGCVR+CSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS
Sbjct: 961  EALAIYHVTYEYAMSHGCVRYCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1020

BLAST of Carg12234 vs. ExPASy TrEMBL
Match: A0A6J1L5Q2 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111499362 PE=3 SV=1)

HSP 1 Score: 1998.8 bits (5177), Expect = 0.0e+00
Identity = 989/1018 (97.15%), Postives = 1006/1018 (98.82%), Query Frame = 0

Query: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60
            MADH HEVPLPPSVEQFLLQIC QQNQPPPDAKARRALASLGEEMALD+LRKISSCS+RN
Sbjct: 1    MADHLHEVPLPPSVEQFLLQICVQQNQPPPDAKARRALASLGEEMALDSLRKISSCSIRN 60

Query: 61   LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120
            LSGFIMHMVRNDPCISPQNKMVAV SPH+SPSSTGCVSQLHSPSTCSASLYQSLSSCRV 
Sbjct: 61   LSGFIMHMVRNDPCISPQNKMVAVFSPHSSPSSTGCVSQLHSPSTCSASLYQSLSSCRVY 120

Query: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180
            SPQGTAEKTKFE STPDKSAIYSS VSDRV+IPQFVALGELEFRRAFLILSYIGGESLER
Sbjct: 121  SPQGTAEKTKFEPSTPDKSAIYSSSVSDRVQIPQFVALGELEFRRAFLILSYIGGESLER 180

Query: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240
            VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRR+YVDWDRRKTHIYHCYVALDGSY
Sbjct: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRVYVDWDRRKTHIYHCYVALDGSY 240

Query: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300
             FKGP+LNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL
Sbjct: 241  SFKGPFLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300

Query: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360
            RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH
Sbjct: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360

Query: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNGNVMYRDGKPLIHTD 420
            PHTV SVASYMARFSLILSKTIKLKIDLSSVNV+RI DIPCKDVNGNVMYRDGKPLIHTD
Sbjct: 361  PHTVSSVASYMARFSLILSKTIKLKIDLSSVNVQRITDIPCKDVNGNVMYRDGKPLIHTD 420

Query: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480
            GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF
Sbjct: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480

Query: 481  RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540
            RLFYNGLAVKGTFLVNKQ PSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA
Sbjct: 481  RLFYNGLAVKGTFLVNKQLPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540

Query: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600
            YISRNLIALLNYGGVPREYFMNILVDALRDVQG+FSSKRAALRVSINNGDMDDFLVARMI
Sbjct: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGLFSSKRAALRVSINNGDMDDFLVARMI 600

Query: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660
            LSGIPL+ESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN
Sbjct: 601  LSGIPLDESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660

Query: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720
            GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD
Sbjct: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720

Query: 721  FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780
            FDGDMYWVSRNSQLLEYFRPG+PWRPSPSMEVI NIK +EFSAEDLEEELFKLFLTARFQ
Sbjct: 721  FDGDMYWVSRNSQLLEYFRPGKPWRPSPSMEVITNIKSNEFSAEDLEEELFKLFLTARFQ 780

Query: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV 840
            PSYAKSVAADNWVALMDQLLMLGEDCKEERNR+RAKILQLINIYYDALDAPKKGGKEIEV
Sbjct: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRLRAKILQLINIYYDALDAPKKGGKEIEV 840

Query: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900
            P+HLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEE+PNIEVQKLPCFE+EVADDTL
Sbjct: 841  PKHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEESPNIEVQKLPCFEDEVADDTL 900

Query: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA 960
            MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRW+EEVYKEA
Sbjct: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWMEEVYKEA 960

Query: 961  LAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1019
            LAIYHVTYEYAMSHGCVR+CSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS
Sbjct: 961  LAIYHVTYEYAMSHGCVRYCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1018

BLAST of Carg12234 vs. ExPASy TrEMBL
Match: A0A6J1L2X5 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111499362 PE=3 SV=1)

HSP 1 Score: 1993.8 bits (5164), Expect = 0.0e+00
Identity = 989/1020 (96.96%), Postives = 1006/1020 (98.63%), Query Frame = 0

Query: 1    MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60
            MADH HEVPLPPSVEQFLLQIC QQNQPPPDAKARRALASLGEEMALD+LRKISSCS+RN
Sbjct: 1    MADHLHEVPLPPSVEQFLLQICVQQNQPPPDAKARRALASLGEEMALDSLRKISSCSIRN 60

Query: 61   LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120
            LSGFIMHMVRNDPCISPQNKMVAV SPH+SPSSTGCVSQLHSPSTCSASLYQSLSSCRV 
Sbjct: 61   LSGFIMHMVRNDPCISPQNKMVAVFSPHSSPSSTGCVSQLHSPSTCSASLYQSLSSCRVY 120

Query: 121  SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180
            SPQGTAEKTKFE STPDKSAIYSS VSDRV+IPQFVALGELEFRRAFLILSYIGGESLER
Sbjct: 121  SPQGTAEKTKFEPSTPDKSAIYSSSVSDRVQIPQFVALGELEFRRAFLILSYIGGESLER 180

Query: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240
            VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRR+YVDWDRRKTHIYHCYVALDGSY
Sbjct: 181  VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRVYVDWDRRKTHIYHCYVALDGSY 240

Query: 241  RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300
             FKGP+LNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL
Sbjct: 241  SFKGPFLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300

Query: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360
            RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH
Sbjct: 301  RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360

Query: 361  PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNGNVMYRDGKPLIHTD 420
            PHTV SVASYMARFSLILSKTIKLKIDLSSVNV+RI DIPCKDVNGNVMYRDGKPLIHTD
Sbjct: 361  PHTVSSVASYMARFSLILSKTIKLKIDLSSVNVQRITDIPCKDVNGNVMYRDGKPLIHTD 420

Query: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480
            GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF
Sbjct: 421  GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480

Query: 481  RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540
            RLFYNGLAVKGTFLVNKQ PSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA
Sbjct: 481  RLFYNGLAVKGTFLVNKQLPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540

Query: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600
            YISRNLIALLNYGGVPREYFMNILVDALRDVQG+FSSKRAALRVSINNGDMDDFLVARMI
Sbjct: 541  YISRNLIALLNYGGVPREYFMNILVDALRDVQGLFSSKRAALRVSINNGDMDDFLVARMI 600

Query: 601  LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660
            LSGIPL+ESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN
Sbjct: 601  LSGIPLDESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660

Query: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720
            GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD
Sbjct: 661  GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720

Query: 721  FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780
            FDGDMYWVSRNSQLLEYFRPG+PWRPSPSMEVI NIK +EFSAEDLEEELFKLFLTARFQ
Sbjct: 721  FDGDMYWVSRNSQLLEYFRPGKPWRPSPSMEVITNIKSNEFSAEDLEEELFKLFLTARFQ 780

Query: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV 840
            PSYAKSVAADNWVALMDQLLMLGEDCKEERNR+RAKILQLINIYYDALDAPKKGGKEIEV
Sbjct: 781  PSYAKSVAADNWVALMDQLLMLGEDCKEERNRLRAKILQLINIYYDALDAPKKGGKEIEV 840

Query: 841  PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900
            P+HLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEE+PNIEVQKLPCFE+EVADDTL
Sbjct: 841  PKHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEESPNIEVQKLPCFEDEVADDTL 900

Query: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKE--ILYGAEELEGSPRWVEEVYK 960
            MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKE  ILYGAEELEGSPRW+EEVYK
Sbjct: 901  MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKELQILYGAEELEGSPRWMEEVYK 960

Query: 961  EALAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1019
            EALAIYHVTYEYAMSHGCVR+CSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS
Sbjct: 961  EALAIYHVTYEYAMSHGCVRYCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQIFCSS 1020

BLAST of Carg12234 vs. ExPASy TrEMBL
Match: A0A6J1EUJ7 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111436744 PE=3 SV=1)

HSP 1 Score: 1865.1 bits (4830), Expect = 0.0e+00
Identity = 930/936 (99.36%), Postives = 932/936 (99.57%), Query Frame = 0

Query: 1   MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60
           MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN
Sbjct: 1   MADHFHEVPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCSVRN 60

Query: 61  LSGFIMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120
           LSGFIMHMVRNDPCISPQNK VAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS
Sbjct: 61  LSGFIMHMVRNDPCISPQNKTVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVS 120

Query: 121 SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180
           SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER
Sbjct: 121 SPQGTAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLER 180

Query: 181 VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240
           VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY
Sbjct: 181 VTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSY 240

Query: 241 RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300
           RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL
Sbjct: 241 RFKGPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGL 300

Query: 301 RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360
           RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH
Sbjct: 301 RRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMH 360

Query: 361 PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNGNVMYRDGKPLIHTD 420
           PHTVPSVASYMARFSLILSKTIKLKIDLSSVNV+RIADIPCKDVNGNVMYRDGKPLIHTD
Sbjct: 361 PHTVPSVASYMARFSLILSKTIKLKIDLSSVNVQRIADIPCKDVNGNVMYRDGKPLIHTD 420

Query: 421 GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480
           GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF
Sbjct: 421 GTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQF 480

Query: 481 RLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540
           RLFYNGLAVKGTFLVNKQ PSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA
Sbjct: 481 RLFYNGLAVKGTFLVNKQLPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRA 540

Query: 541 YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600
           YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI
Sbjct: 541 YISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMI 600

Query: 601 LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660
           LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN
Sbjct: 601 LSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILEN 660

Query: 661 GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720
           GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD
Sbjct: 661 GQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGD 720

Query: 721 FDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780
           FDGDMYWVSRNSQLLEYFR GEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ
Sbjct: 721 FDGDMYWVSRNSQLLEYFRAGEPWRPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQ 780

Query: 781 PSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEV 840
           PSYAKSVAADNWVALMDQLL+LGEDCKEERN VRAKILQLINIYYDALDAPKKGGKEIEV
Sbjct: 781 PSYAKSVAADNWVALMDQLLVLGEDCKEERNLVRAKILQLINIYYDALDAPKKGGKEIEV 840

Query: 841 PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900
           PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL
Sbjct: 841 PEHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQEEAPNIEVQKLPCFEEEVADDTL 900

Query: 901 MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKY 937
           MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKY
Sbjct: 901 MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKY 936

BLAST of Carg12234 vs. TAIR 10
Match: AT2G19930.1 (RNA-dependent RNA polymerase family protein )

HSP 1 Score: 879.4 bits (2271), Expect = 2.8e-255
Identity = 481/1025 (46.93%), Postives = 668/1025 (65.17%), Query Frame = 0

Query: 8    VPLPPSVEQFLLQICTQQNQPPPDAKARRALASLGEEMALDALRKISSCS---VRNLSGF 67
            + L   +E  L  I  + N  P + + R+ L+S+ E +  + +RK+ S     + NL  F
Sbjct: 10   IALSGRIETALENIYRKHNLTPINDETRQRLSSIPENLGFELVRKVFSLQAGLIYNLDSF 69

Query: 68   IMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVSSPQG 127
            I         +S  N+ V         S TG     +S S     + + L          
Sbjct: 70   I---------VSKVNQAV---------SFTGYPQPANSLSPSGRHVSRVLQ--------- 129

Query: 128  TAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLERVTTA 187
              E+   ++  P   ++ S      + IPQ VALGELEF++ FL+LSYI G+ + +V TA
Sbjct: 130  --EEMSVDSDAPSPKSLKSEDKGGSLHIPQLVALGELEFKKVFLLLSYIPGQHVGQVITA 189

Query: 188  DQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSYRFK- 247
            D+I+    L M ++E+ VW   G  Y  ++DRRM + WD  KTH Y C VA +GSY FK 
Sbjct: 190  DEIRLWKDLPMVEYEAAVWDRLGRHYCPQKDRRM-LQWDSGKTHYYQCNVAPNGSYTFKV 249

Query: 248  -----GPYLNHTKTHLQRVLGDDNVLMVKFA--EDVSDRCSISHPGGSFYAYKKIARDGI 307
                 GP L HT THL +VLGDDNVL VKFA  +  S   SI H    ++ YK IA++GI
Sbjct: 250  LSALQGPLLEHTGTHLHKVLGDDNVLTVKFADVQKSSSTYSIDH----YFTYKGIAKNGI 309

Query: 308  LLGLRRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARS 367
            ++GLRRYQFFVFKDGGKEEKKK+ +T  VKCYF+R +S A++D   PY L+ ++I+EAR 
Sbjct: 310  MIGLRRYQFFVFKDGGKEEKKKDLSTKKVKCYFIRTDSTAFYDMQNPYILTGKSIYEARM 369

Query: 368  LFMHPHTVPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNG-NVMYRDGKP 427
             FMH H  P++A+YMARFSLILSKT  L++D++ +   +I DI C D +G +V+ ++ KP
Sbjct: 370  HFMHVHRAPTLANYMARFSLILSKTKTLEVDMTGITFDQIDDIHCHDQDGKDVLDKNKKP 429

Query: 428  LIHTDGTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPP 487
             IH+DGTG+ISEDLA  CP+N+FKG+      ++                      ++PP
Sbjct: 430  CIHSDGTGYISEDLARMCPLNIFKGKCLRSESIQEAC------------------YQDPP 489

Query: 488  LLIQFRLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSN 547
            LLIQFR+FY+G AVKGTFL+NK+   RT+Q+R SMIKV KDP+L N  T N+LEVV TSN
Sbjct: 490  LLIQFRMFYDGYAVKGTFLLNKKLCPRTVQVRPSMIKVSKDPSLSNFSTFNALEVVTTSN 549

Query: 548  PPKRAYISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFL 607
            PPKR  +S+NL+ALL+YGG+P E+F++IL++ L + + +F +KRAAL  ++N G+MDD  
Sbjct: 550  PPKRTKLSKNLVALLSYGGIPNEFFLDILLNTLEESKSIFYNKRAALNAALNYGEMDDQN 609

Query: 608  VARMILSGIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVC 667
             A+MIL GIPL+E +L+  LS+LL+ EK  L++G+L V + YYLMGT DPT  L+ +EVC
Sbjct: 610  AAQMILVGIPLDEPHLKNYLSILLKTEKNDLKAGKLPVTESYYLMGTVDPTGALKEDEVC 669

Query: 668  VILENGQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADE 727
            VILE+GQI+G+VLVYRNPGLHFGDIH+L A YV+ L   VGN+K+ +FF  KGPRS+ DE
Sbjct: 670  VILESGQISGEVLVYRNPGLHFGDIHILKATYVKALEEYVGNSKFAVFFPQKGPRSLGDE 729

Query: 728  IAGGDFDGDMYWVSRNSQLLEYFRPGEPW-RPSPSMEVIANIKPSEFSAEDLEEELFKLF 787
            IAGGDFDGDMY++SRN +LLE F+P EPW   +P  +  +   PS+ S E+LEEELF++F
Sbjct: 730  IAGGDFDGDMYFISRNPELLENFKPSEPWVSLTPPSKSNSGRAPSQLSPEELEEELFEMF 789

Query: 788  LTARFQPSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKG 847
            LTA F  S    +AAD+W+ +MD+ L+LG+D  EE+  ++ K+L+LI+IYYDALDAPKKG
Sbjct: 790  LTAGFHASNVIGIAADSWLTIMDRFLILGDDRAEEKAEMKKKMLELIDIYYDALDAPKKG 849

Query: 848  GKEIEVPEHLKAGTFPHFMERGKKSYTSTSILGQIFDTV--NMFQEEAPNIEVQKLPCFE 907
             K + +P  LK   FPH+MER KK + STSILG IFD V     +E +P+ E+ KLPCFE
Sbjct: 850  DK-VYLPNKLKPDIFPHYMERDKK-FQSTSILGLIFDFVKSQTTEEPSPSSEISKLPCFE 909

Query: 908  EE-VADDTLMKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPR 967
            +E V++  + K +  Y+ YR EM  A++ D   K+ +A   I++YK+  YGA   E S +
Sbjct: 910  DEPVSEFHMQKCRLWYDNYRTEMTQAMKTD---KDESANEVIQRYKQEFYGAAGFEDSKK 969

Query: 968  WVEEVYKEALAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNL-CARKHSENSFLCLPSV 1016
             +EE+Y +ALA+Y + Y+YA+ H  V  C F W+VAG  L      +K  E   +C PSV
Sbjct: 970  SLEELYPQALALYKIVYDYAI-HAGVSKCRFVWKVAGPVLCRFYLNKKMQEKCLVCAPSV 976

BLAST of Carg12234 vs. TAIR 10
Match: AT2G19910.1 (RNA-dependent RNA polymerase family protein )

HSP 1 Score: 862.8 bits (2228), Expect = 2.7e-250
Identity = 482/1017 (47.39%), Postives = 657/1017 (64.60%), Query Frame = 0

Query: 7    EVPLPPSVEQFLLQICTQQN-QPPPDAKARRALASLGEEMALDALRKI-SSCSVRNLSGF 66
            E+ L  SVE  L +I  + N + P   + RR L+S+ EE+AL+ LRK+ +   ++ L G 
Sbjct: 14   EIALLGSVETMLEKIYEKHNHRLPISVETRRKLSSISEELALETLRKVFNKPYLKTLDGL 73

Query: 67   IMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVSSPQG 126
            IM+ V+    +          SP  SP      S + SP T       +  SCR S    
Sbjct: 74   IMYFVKGTVTVDG--------SPRLSPGE----SPVQSPRT------PAKKSCRAS---- 133

Query: 127  TAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESL-ERVTT 186
              +    +  TP    +          IP  +ALGELEF++AFL+LSYIGGESL E V +
Sbjct: 134  --QDVSLDLETPSPKFMKREENGGSKYIPPLLALGELEFKKAFLLLSYIGGESLVEEVIS 193

Query: 187  ADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSYRFK 246
             DQI+    L M  +E+ VW   G++Y + ++RR  ++ D   TH Y C+VA DGSY+FK
Sbjct: 194  GDQIRKWKDLPMVSYEAAVWNRLGQRYCSPKERRRPLEGDSGMTHYYQCHVATDGSYKFK 253

Query: 247  GPYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGLRRY 306
            G  L +T THL +VLGDDNVL VKF + +      ++    +  YK IA++GI++GLRRY
Sbjct: 254  GHLLENTGTHLHKVLGDDNVLTVKFDKVLGVE---TYCNDLYSTYKGIAKNGIMVGLRRY 313

Query: 307  QFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMHPHT 366
            +FFVFKDGGKEEKKK+ +T  VKCYF+R +S A  D   PY  + +++ EAR  FMH +T
Sbjct: 314  RFFVFKDGGKEEKKKDVSTKGVKCYFIRTDSTASIDMQNPYIFAGKSMHEARMHFMHVNT 373

Query: 367  VPSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKDVNG-NVMYRDGKPLIHTDGT 426
            + S+ +YM+RFSLILSKT  L++D++ +   +I DI C D +  +V+ ++GKP IH+DGT
Sbjct: 374  LSSLPNYMSRFSLILSKTKTLEVDMTGITFEQIDDIHCHDQDDKDVLDKNGKPCIHSDGT 433

Query: 427  GFISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQFRL 486
            G+ISEDLA  CP+N+FKG++    ++         Q+K       G   +EPPLLIQFR+
Sbjct: 434  GYISEDLARMCPVNIFKGKSMRSNNI---------QSKNLNFEGQGPCGQEPPLLIQFRI 493

Query: 487  FYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRAYI 546
            FYNG AVKGTFL NK+ P RT+Q+R SMIKV +D  L N  T NSLEVV TSNPP++A +
Sbjct: 494  FYNGYAVKGTFLTNKKLPPRTVQVRPSMIKVYEDRTLSNLSTFNSLEVVTTSNPPRKARL 553

Query: 547  SRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMILS 606
            SRNL+ALL+YGGVP ++F+NIL + L + + +F S+RAA + +IN G  DD   A MIL 
Sbjct: 554  SRNLVALLSYGGVPNDFFLNILRNTLEESKTIFYSERAAFKAAINYG--DDQYTADMILV 613

Query: 607  GIPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILENGQ 666
            GIPL+E YL+ RLS LL+ E+ +L++GR  + + YY+MGT DPT  L+ NE+CVIL +GQ
Sbjct: 614  GIPLDEPYLKDRLSYLLKTERNALKAGRFPIDESYYIMGTVDPTGELKENEICVILHSGQ 673

Query: 667  INGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGDFD 726
            I+G VLVYRNPGLHFGDIHVL A YV+ L   VGNAK+ +FF  KGPRS+ DEIAGGDFD
Sbjct: 674  ISGDVLVYRNPGLHFGDIHVLKATYVKALEDYVGNAKFAVFFPQKGPRSLGDEIAGGDFD 733

Query: 727  GDMYWVSRNSQLLEYFRPGEPW-RPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQP 786
            GDMY++SRN +LLE+F+P EPW   S   ++    KPSE S E+LEEELFK+FL ARF  
Sbjct: 734  GDMYFISRNPKLLEHFKPSEPWVSSSKPSKIYCGRKPSELSEEELEEELFKMFLKARFCK 793

Query: 787  SYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEVP 846
                 +AAD W+ +MD  L LG++  +E+   +  IL+LI+IYYDALDAPKKG K +++P
Sbjct: 794  RDVIGMAADCWLGIMDPFLTLGDESAKEKYERKKNILKLIDIYYDALDAPKKGAK-VDLP 853

Query: 847  EHLKAGTFPHFMERG-KKSYTSTSILGQIFDTVNMFQ-EEAPNIEVQKLPCFEEE-VADD 906
              L+   FPH+MER  K+ + STSILG IFDTV+    EE P  E+ KL  FE+E V   
Sbjct: 854  PDLEIKNFPHYMERDPKRDFRSTSILGLIFDTVDSHNAEEPPPSEISKLWYFEDEPVPKS 913

Query: 907  TLMKWKFQYEMYRKEMRDA-LQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVY 966
             + K+   YE YR EM  A ++ D   +N      I++YK+  YGA   E S + +EE+Y
Sbjct: 914  HMDKFTSWYENYRSEMSQAMMETDKVKRNQLTNEVIQRYKQDFYGAAGFEDSNKSLEELY 973

Query: 967  KEALAIYHVTYEYAMSHGCVRHCSFAWRVAGSALFNLCARKHSENSFLCLPSVMRQI 1015
             +ALA+Y+V Y+YA+  G V  C+FAW VAG  L     +K  + S +   SV++++
Sbjct: 974  PQALALYNVVYDYAIQEG-VAKCTFAWNVAGPVLCKFYLKKTKDKSVVASTSVLKKL 990

BLAST of Carg12234 vs. TAIR 10
Match: AT2G19920.1 (RNA-dependent RNA polymerase family protein )

HSP 1 Score: 797.3 bits (2058), Expect = 1.4e-230
Identity = 442/967 (45.71%), Postives = 618/967 (63.91%), Query Frame = 0

Query: 7   EVPLPPSVEQFLLQICTQQNQPPP-DAKARRALASLGEEMALDALRKISSC-SVRNLSGF 66
           E+ L  SVE  L ++  + N  PP   + RR L+S+ EE+AL+ LRK+ +  +V+ L G 
Sbjct: 19  EIALLGSVETMLEKVYGKHNHHPPIKVETRRRLSSISEELALETLRKVLNMPNVKTLDGI 78

Query: 67  IMHMVRNDPCISPQNKMVAVLSPHTSPSSTGCVSQLHSPSTCSASLYQSLSSCRVSSPQG 126
           I++ + +   +    ++ +  SP   P + G                    SCR S  + 
Sbjct: 79  IIYFLNDAVTVDGSPRLWSGESPVQFPRTPG------------------KKSCRASQAE- 138

Query: 127 TAEKTKFEASTPDKSAIYSSLVSDRVKIPQFVALGELEFRRAFLILSYIGGESLERVTTA 186
                  +   P    +      +   I   +ALGELEF++AFL+L+Y+GG+SL  V + 
Sbjct: 139 ----VSLDREDPSPKFLRGDENGESKHISLLLALGELEFKKAFLLLTYLGGKSLGEVISG 198

Query: 187 DQIQSLSQLAMEKFESEVWKAFGEKYVNKEDRRMYVDWDRRKTHIYHCYVALDGSYRFKG 246
           D+I+    L M  +E  VW   G+      + RM ++ D  KTH Y C+VA DGSYR KG
Sbjct: 199 DEIRQWKDLPMVAYERAVWFKLGQ-----NEERMQLESDSGKTHYYQCHVAPDGSYRLKG 258

Query: 247 PYLNHTKTHLQRVLGDDNVLMVKFAEDVSDRCSISHPGGSFYAYKKIARDGILLGLRRYQ 306
            +L +T THL +VLGDDNVL V+F +   +     +P   +  YK+IA++GI++GLRRYQ
Sbjct: 259 YFLENTGTHLHKVLGDDNVLTVRFDQLPKESTYCDNP---YSKYKEIAKNGIMVGLRRYQ 318

Query: 307 FFVFKDGGKEEKKKNPTTSAVKCYFVRMESAAYFDKLQPYKLSNRTIFEARSLFMHPHTV 366
           FFVFKDGGK EKKK  +T  VKCYF+R  S A  D   PY LS  +I EAR  FMH HT+
Sbjct: 319 FFVFKDGGKAEKKKRNSTKQVKCYFIRTGSTASSDMENPYILSGMSIHEARMHFMHVHTL 378

Query: 367 PSVASYMARFSLILSKTIKLKIDLSSVNVRRIADIPCKD-VNGNVMYRDGKPLIHTDGTG 426
           PS A+YMARFSLILSKT KL++D++ + V +I DI C D  N +V+ ++GKP IH+DGTG
Sbjct: 379 PSPANYMARFSLILSKTKKLEVDMTEITVMQIDDIHCHDQSNNDVLDKNGKPRIHSDGTG 438

Query: 427 FISEDLALECPMNVFKGQAKHDADLKRIPAFEVFQNKTSQVILPGLELREPPLLIQFRLF 486
           +ISEDLA  CP+N+FKG++    +++                  G  ++EPPLLIQ R+F
Sbjct: 439 YISEDLARMCPLNIFKGKSMRSNNIQ------------------GTCVQEPPLLIQIRMF 498

Query: 487 YNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKRAYIS 546
            +G AVKG FL+NK  P +T+Q+R SMIKV KD NL N  T NSLEVV TSNPPKRA +S
Sbjct: 499 NDGSAVKGIFLLNKNLPPQTVQVRPSMIKVYKDKNLSNFSTFNSLEVVTTSNPPKRAKLS 558

Query: 547 RNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLVARMILSG 606
           +NL+ALL+YGGVP ++F++IL++ L   + +F   RAA + +++ G+MDD    +MI++G
Sbjct: 559 KNLVALLSYGGVPNDFFLDILLNTLEKKKTIFFKVRAAGKAALHYGNMDDKNALQMIMAG 618

Query: 607 IPLNESYLQYRLSMLLREEKKSLRSGRLHVPDCYYLMGTADPTCTLESNEVCVILENGQI 666
           IPL+E YL++ LS LL+ EK  L++G+L + + YYLMGT DPT  L+ +EV  + ++   
Sbjct: 619 IPLDEPYLKHYLSKLLKLEKDDLKAGKLPIDESYYLMGTVDPTGELKEDEVSGLAKSQD- 678

Query: 667 NGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKGPRSVADEIAGGDFDG 726
              VLVYRNPGLHFGDIH+L A YV+ L   VGN+KYG+FF  KGPRS+ DEIAGGDFDG
Sbjct: 679 ---VLVYRNPGLHFGDIHILKATYVKSLEQYVGNSKYGVFFPQKGPRSLGDEIAGGDFDG 738

Query: 727 DMYWVSRNSQLLEYFRPGEPW-RPSPSMEVIANIKPSEFSAEDLEEELFKLFLTARFQPS 786
           DMY++SRN +LLE+++P EPW   SP  ++    +PSE S E LEEELFK+FL   F PS
Sbjct: 739 DMYFISRNPKLLEHYKPSEPWVSSSPRSKIYTGRQPSELSPEQLEEELFKIFLKTGFSPS 798

Query: 787 YAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYDALDAPKKGGKEIEVPE 846
                AAD+W+A+MD+ L LG++  +E+  ++ K+L+L +IYYDA+DAPK  G E+ +P 
Sbjct: 799 SVIGQAADSWLAIMDRFLTLGDENVKEKAEMKKKMLKLTDIYYDAIDAPKT-GTEVNLPL 858

Query: 847 HLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMFQ-EEAPNIEVQKLPCFEEE-VADDTL 906
            +K   FPH+MER  K++ STSILG IFDTV+    E+     + KL CFE+E V++  +
Sbjct: 859 DVKVDLFPHYMER-NKTFKSTSILGLIFDTVDFHNAEDTTPSGISKLQCFEDEPVSEFDM 918

Query: 907 MKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKYKEILYGAEELEGSPRWVEEVYKEA 966
            K K  ++ YRKEM  A+  D D    +    I+KYK+  Y A   + S + +EE+Y +A
Sbjct: 919 EKCKLWHKDYRKEMCQAMNSDDDD---SCNEVIQKYKQEFYSAAGFKESKKILEELYPKA 927

Query: 967 LAIYHVT 968
           LA+Y+VT
Sbjct: 979 LALYNVT 927

BLAST of Carg12234 vs. TAIR 10
Match: AT4G11130.1 (RNA-dependent RNA polymerase 2 )

HSP 1 Score: 105.5 bits (262), Expect = 2.5e-22
Identity = 145/578 (25.09%), Postives = 241/578 (41.70%), Query Frame = 0

Query: 480  FRLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKR 539
            F++ Y G   KG   V++    R + +R+SM+K + +  + N           T + P  
Sbjct: 581  FQIRYGG--YKGVIAVDRS-SFRKLSLRDSMLKFDSNNRMLNVTR-------WTESMP-- 640

Query: 540  AYISRNLIALLNYGGVPREYFMNILVDALRDVQGVFSSKRAALRVSIN-NGDMDDFLVAR 599
             +++R +I LL+  G+    F  +    L  +  +   + AAL V    +G+    L+ +
Sbjct: 641  CFLNREIICLLSTLGIEDAMFEAMQAVHLSMLGNMLEDRDAALNVLQKLSGENSKNLLVK 700

Query: 600  MILSG-IPLNESYLQYRLSMLLREEKKSLRSG-RLHVPDCYYLMGTADPTCTLESNEVCV 659
            M+L G  P +E YL   L +    +   L+S  R+ VP    L+G  D    LE  +V V
Sbjct: 701  MLLQGYAPSSEPYLSMMLRVHHESQLSELKSRCRILVPKGRILIGCMDEMGILEYGQVYV 760

Query: 660  IL--------------------ENGQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVG 719
             +                    E   + GKV+V +NP LH GDI VL A Y E      G
Sbjct: 761  RVTLTKAELKSRDQSYFRKIDEETSVVIGKVVVTKNPCLHPGDIRVLDAIY-EVHFEEKG 820

Query: 720  NAKYGIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEVIANI 779
                 I F  KG R   +E +GGD DGD ++VS + +++       P    P M+  A  
Sbjct: 821  YLDC-IIFPQKGERPHPNECSGGDLDGDQFFVSWDEKII-------PSEMDPPMD-YAGS 880

Query: 780  KPSEFSAEDLEEELFKLFLTARFQPSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAK 839
            +P     +   EE+ K F+      +          V     L+    D ++ R++   K
Sbjct: 881  RPRLMDHDVTLEEIHKFFVDYMISDTLG--------VISTAHLVHADRDPEKARSQ---K 940

Query: 840  ILQLINIYYDALDAPKKGGKEIEVPEHLKAGTFPHFMERGKK-SYTSTSILGQIFDTV-N 899
             L+L N++  A+D  K G    E+P  LK   FP F+ER +K +Y S S+ G+++  V +
Sbjct: 941  CLELANLHSRAVDFAKTGA-PAEMPYALKPREFPDFLERFEKPTYISESVFGKLYRAVKS 1000

Query: 900  MFQEEAPNIEVQKLPCFE---EEVADDTLMKW-KFQYEMYRKEMRDALQLDPDAKNVAAE 959
               +  P  E +    ++   EE   ++ ++  K   +MY +++   +     A      
Sbjct: 1001 SLAQRKPEAESEDTVAYDVTLEEAGFESFIETAKAHRDMYGEKLTSLMIYYGAANEEEIL 1060

Query: 960  ATIKKYKEIL-------YGAEELEGSPRWVEEVYKEAL------------------AIYH 1004
              I K KE+        YG +  +     V++++KEA+                  A Y+
Sbjct: 1061 TGILKTKEMYLARDNRRYG-DMKDRITLSVKDLHKEAMGWFEKSCEDEQQKKKLASAWYY 1120

BLAST of Carg12234 vs. TAIR 10
Match: AT1G14790.1 (RNA-dependent RNA polymerase 1 )

HSP 1 Score: 97.8 bits (242), Expect = 5.2e-20
Identity = 130/520 (25.00%), Postives = 219/520 (42.12%), Query Frame = 0

Query: 480  FRLFYNGLAVKGTFLVNKQFPSRTIQIRNSMIKVEKDPNLENAETENSLEVVGTSNPPKR 539
            F++ Y G   KG   V+    S+ + +R SM K E +           L+V+  S   + 
Sbjct: 563  FQIRYGG--YKGVVAVDPN-SSKKLSLRKSMSKFESE--------NTKLDVLAWSK-YQP 622

Query: 540  AYISRNLIALLNYGGVPREYF---MNILVDALRDVQGVFSSKRAALRVSINNGDMDDFLV 599
             Y++R LI LL+  GV    F      +VD L  +         AL + +  G+  + L 
Sbjct: 623  CYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPLEAHEALGL-MAPGENTNILK 682

Query: 600  ARMILSGIPLNESYLQYRLSMLLREEKKSLRS-GRLHVPDCYYLMGTADPTCTLESNEVC 659
            A ++    P  E +L   L      +   LR+  R+ +     +MG  D T TLE  +V 
Sbjct: 683  ALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGGRSMMGCLDETRTLEYGQVV 742

Query: 660  VILENGQ-------INGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYGIFFSCKG 719
            V   +         I G V+V +NP LH GD+ VL A      VP + +    + F  KG
Sbjct: 743  VQYSDPMRPGRRFIITGPVVVAKNPCLHPGDVRVLQAVN----VPALNHMVDCVVFPQKG 802

Query: 720  PRSVADEIAGGDFDGDMYWVSRNSQLLEYFRPGEP--WRPSPSMEVIANIKPSEFSAEDL 779
             R   +E +G D DGD+Y+V  + +L+   R  EP  + P P+      I   + + E++
Sbjct: 803  LRPHPNECSGSDLDGDIYFVCWDQELVPP-RTSEPMDYTPEPT-----QILDHDVTIEEV 862

Query: 780  EEELFKLFLTARFQPSYAKSVAADNWVALMDQLLMLGEDCKEERNRVRAKILQLINIYYD 839
            EE        A +  + +  + A+   A  D         KE         ++L   +  
Sbjct: 863  EEYF------ANYIVNDSLGIIANAHTAFAD---------KEPLKAFSDPCIELAKKFST 922

Query: 840  ALDAPKKGGKEIEVPEHLKAGTFPHFMER-GKKSYTSTSILGQIFDTVNMFQEEAPNI-- 899
            A+D PK G   + +P+HL    +P FME+  K +Y S +++G++F  V   +E AP +  
Sbjct: 923  AVDFPKTGVAAV-IPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREV---KERAPPLIS 982

Query: 900  --------------EVQKLPCFEEEVADDTLMKWKFQYEMYRKEMRDALQLDPDAKNVAA 959
                          +  ++  FEE V  D     K  Y+     + D   +  +A+ ++ 
Sbjct: 983  IKSFTLDVASKSYDKDMEVDGFEEYV--DEAFYQKANYDFKLGNLMDYYGIKTEAEILS- 1036

Query: 960  EATIKKYKEILYGAEELEGSPRWVEEVYKEALAIYHVTYE 970
               I +  +      + E   R V  + KE L++++ + E
Sbjct: 1043 -GGIMRMSKSFTKRRDAESIGRAVRALRKETLSLFNASEE 1036