Carg12184 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12184
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	nudix hydrolase 20, chloroplastic-like
Location	Carg_Chr11: 2608016 .. 2612289 (-)
RNA-Seq Expression	Carg12184
Synteny	Carg12184

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGACTTGTCCTCGAATTCAACCCATGGCCTGCAAATCTCTTGCCTCTCACCGATTTCGTAACTCTTCGCCTATCTCTTCCATTTTCAACGCATCCATGTTTTCCCGGGCGACGTATCCAACGGCATCAACAGTACACCGCTCGAAGACCTTCGCCTGTCGTTCTGTATCGGTTGCCAGCAGCAATTTTACTTGGGAAGACGCTGTCCACATTTCTCTGCCAGAAAGCTTTCAGGATGATCCCTCAGATCTTACCGGCTATTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCAGTAGGTCTCAAAGCCATATGAGTTTCAGCGGCCATTGTGCGCGTTAATTCGTTTTACATTTCAAGTTCTTTGCAGGATCAGTTATAGACTGATGAAATGTGGTTCCATGTCTGTTTGGAATTGACTGGATCTTCTAACAGGATATGCAATCCGAATTTCTTCCATTTGTAATCGATGACCAAATTGTTGGTCATGTGCACCACGGGTGAGGTTCCTACTGTTATATCTTTCAGGATACAGTACATACATCAAAGATATGATCCGTCCATGTAGTTTATACGATTGATTCGTACTAGTTTCATCAAATTTTGCTTTTATGCTGTTGCTATACACAACGCATAGGTTCCATCCGTTTGTTTTAATGTAATTTATGCAGCTTTGCTTATCACCTGAAGAAGTATGCGAATGTGTTTACCTTCCCCCGGGATGATTCCGTTCGAGTTGGCGCTTGCTTAGCTTAGGCACTGAAATCGCATATTGACAAAAAGCACAAAGAGCCTTAAAGACAAAATATGGAGACGGCGCAAGTGTCCAATTATGTTAGCGGAAAGATAGCGGATACAAAAGATTCATATCCGCTTTGTTTTGTTGTTGGGAGAGATCCTCTGTTAGTATTAAATTGAAAAAGGGTTATCAATCTGAGGTAATAATGACGGCACAAATCGGAAAATTCAATAATAGAAAAAGACTCGGCGAGAGCAGAGAGTCGAAAATTGCCATGGTCGGCTGAACATTATAATATGGGACGTATGTCACTTACCTTAACAACGGTAGTAAACTAGAGTGAAGTAGGTGATTGTGATTCCAATTAATATTAAATGAACTTAATGGTCACAATGATATGAACATTATAGTCATGAGGAATAACTCAAACTTAGTGTGTAATCGAATTCTGTTATATTACAATATAAGCGATAAGCTACGATTTTCCACTCAGTCTTAATTGTCAAATATTAAAAGAACATGAGTATATTCTTTTTCTTAGCATACATATTGTAAACCTTACCGAGAACAACGCACTTTGGGAATAGCTACGTGTATGAGTTGGATCAACTGTGTTAAATTCACGGCGACTAGCCACTCACGACAAAAGAGAATTGTAGCCACCAAATGAAAAGCTTCATACGAGTCATACGTCTCTACGTTGCGGGATACACGGTTTTTTGGGTTTTTATCTCGTCACCATTACTTTGGTAACGTGCATGTGGACAGCCTTCCTTATTTAGCGAAATCACACGTATGGAGGAAATGATATGACTCCACTCCTTTCGCAACACAGACACACTTGGAAAACACTATCTAAAAGATATTATTTCCTTGTTTCTCAGTTTATTTGTGCTATATTCCCCACGCATACACTGTTTGGTACGTGAGGTATTTTTTCATTTGAAAACTTCAGCTTATAACCTAGCACATTTGTTGACCTACTTGATATGGTAACATATTCTATGTTATGATATATGTGATTTATTCTTTATCGATGATAATGTAAAGCATCTGTCCTCTGCTAATACCATTAGGTCAGAAAAAAATAATCTTTTATTGAAAAAATTCCCACAATATGCACATATTTCCCTGATCCAACTAATTTATCTTTCTGAACAATAATGAAATTATAAGGGTAATATTTGTTTCTCATAAGTAGAACTATTCTAGTTATCTGGATTATCACACCAGAGACTTGAGCTCTCGTTATATTAGGTCCTAAAATTTGAATACTATCAAGTACACTTCTGATATTATATTTGATAGTACCGAGTCTATGTACTTGAGCGGAATATAAATAAAACAAAGAGTGAGTACATAATTGAGTTATTAATCGTTTTTCAACACACTATAACATTAATGCAATTATTGCTGATTGTACAATGGACATTCGAGGGAGGAACTTTGAGATTCTTATGATATCCTACTCTCTTTGAATAAATATAAATGAAAATTCAATTTTTTGAAGTACTAAGTACTACTTCTCCCTAAATCTTTTCTATATCTTCCGGTCTAGCAAAGAGAACTGAATCTCCGCGTCTGATTAAATAATTATGATATCAACAAGTTGCTCATTCTGGTATGTTCAGATGAATGGTTATGTTGAAAAGGATGGAAAGAAATTTTTGTGGATAGCAAAAAGAAGTCAAATGAAGCAAACTTTTCCTGGAATGCTGGATGAATTAGTAGCTGGAGGGCTGGTTTGTAATCAATAATAACTAACTACCGTTTAGTTTAGTTATATGTCATGTCAAACATGCTTCTGTCTCGTTAAAATGTGAGTAAAAGTTGATTGGCAAACTCCTTGCATCTTTCAAACAGAGCTGTGCAATCTTTTACCATTGATTGAGGAAAAGACTTCCCTTTTGTTTCTGAATGGTTGGTTTTCTTATTATATTTTGTTTCTTCTGCTAGAGGCTTGTAGTCATATGAAGACATTTTTCTCGTATGACATTTCTTAGTCAATGTGGATTTACCTGCTTTATTAAGCTTAGTCAACGTAGCCAATAATAATTTATTTTTATTTGTGTTTGGCTTTCAGTTTGAACGAAAAGATTGTATAGTTAATAATAAAAGTAGATAGATTCTGACACTGATACAATCATTGACTTGGCTATAAATCCTTATCTAAATAACAGGGCACTGTATTTGTGGTTGAGACCAAGATGCGAAGTTTTATAATTTCCTAAGAGCATTAGAGTGTCTGCACAAACATGTTTTAATCAGTAAATTGAGGAATGAAAATGCAAGGCTTAATAATGATGAGGAAAAAGAACCAAGGGAGATGAGAAGTTAGAAATGTGCACTTGATTGCTGAGCATCATGCTTATTGTACCTTTATAGCTTGCCCCTGACTTTATAATGGTGACAGCCTGAGGGCGTTTCTTGCGAGGAGAATCTGATGAAGGAATGTGAAGAGGAAGCAGGCATACCAAGATCAATATCAAATAAGTAAGTTCCTAACATGAACTTAATTAGAAATTCACACCAATCTTGTATTACATGCTAGTGGTATTTTTTTTAATTTATTATTATTATTATTTTTTTTTAGTTCAACAACATGTCAGAGGTCGAGGTTATATGCCATAAGTTTAACTAGTCAAACTGGCTTTATAATGCTTTTGTTTCAGAGCCATACCCGTTGGTGCTGTTTCCTATTCAGACATCAAAGATTATAGTTACAAGAGAGATGTTCTATTTTGTTACGATTTAAAGCTTCCTGAAAGTTTTATACCGAAGAATCAAGGTTAGTTAACGTGATAAATTTTCATTTACTTAATCATTCAAAACTTAGACGAGGGATCTTCCGTTGCACTGAATCCAGACTGGCTCCAAATAACTCTCTTTTTTGCCACGTCCCCGGTGCTTGAGTGCACCAATATATTTTTCATCCAAATTTTTGTCTAGTTAGATATTCCATCCACTAAATTTTGCTGACTAAACTTTCGGCAGATGGGGAGGTTGAAGGATTCAGGTTGTTGCCCGTGACAAATGTTGCAAACGTGATTCGGAGGACACAGTTATTCAAGCATAATTGTGCTCTCGTCATCATTGACTTCCTATTTCGACATGGGTATTATATAATATAATACTATTTTCTCTATGGCTATAAATTTGTTACTCGACCATTATCTGCTTAAGTTACAACACCAAGTGTTGATCTTGATGATAAAAATTTGCGGATTCAACCAAATTGTCAAGGGGACGAAATTAAAAAGTTACTGTAAATTTAGACTACCAAATATTGTTAAAGCAGAAGGATTGGTAGTCCTTTTCTTTCTCTGGGTCCTTACTCCTGATCATTTTGATCAAATCTTTTACCCATCTGTGTACCCAATCCCATTGGCCATTACTTCTCAAGGTATCCTTAAGAGTACAGAGTTGTTTGTAACTAACGTATTTTTCTTTTGAAAACTTGTAGGTACATTAAACCCGAAAGTTTGGGGTACTTGGAGCTACTACAAAGTTTGAAGAGTGGAACCTGCTTCTAA

mRNA sequence

ATGGCGACTTGTCCTCGAATTCAACCCATGGCCTGCAAATCTCTTGCCTCTCACCGATTTCGTAACTCTTCGCCTATCTCTTCCATTTTCAACGCATCCATGTTTTCCCGGGCGACGTATCCAACGGCATCAACAGTACACCGCTCGAAGACCTTCGCCTGTCGTTCTGTATCGGTTGCCAGCAGCAATTTTACTTGGGAAGACGCTGTCCACATTTCTCTGCCAGAAAGCTTTCAGGATGATCCCTCAGATCTTACCGGCTATTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCAGATATGCAATCCGAATTTCTTCCATTTGTAATCGATGACCAAATTGTTGGTCATGTGCACCACGGCTTTGCTTATCACCTGAAGAAGTATGCGAATGTGTTTACCTTCCCCCGGGATGATTCCGTTCGAATGAATGGTTATGTTGAAAAGGATGGAAAGAAATTTTTGTGGATAGCAAAAAGAAGTCAAATGAAGCAAACTTTTCCTGGAATGCTGGATGAATTAGTAGCTGGAGGGCTGCCTGAGGGCGTTTCTTGCGAGGAGAATCTGATGAAGGAATGTGAAGAGGAAGCAGGCATACCAAGATCAATATCAAATAAAGCCATACCCGTTGGTGCTGTTTCCTATTCAGACATCAAAGATTATAGTTACAAGAGAGATGTTCTATTTTGTTACGATTTAAAGCTTCCTGAAAGTTTTATACCGAAGAATCAAGATGGGGAGGTTGAAGGATTCAGGTTGTTGCCCGTGACAAATGTTGCAAACGTGATTCGGAGGACACAGTTATTCAAGCATAATTGTGCTCTCGTCATCATTGACTTCCTATTTCGACATGGGTACATTAAACCCGAAAGTTTGGGGTACTTGGAGCTACTACAAAGTTTGAAGAGTGGAACCTGCTTCTAA

Coding sequence (CDS)

ATGGCGACTTGTCCTCGAATTCAACCCATGGCCTGCAAATCTCTTGCCTCTCACCGATTTCGTAACTCTTCGCCTATCTCTTCCATTTTCAACGCATCCATGTTTTCCCGGGCGACGTATCCAACGGCATCAACAGTACACCGCTCGAAGACCTTCGCCTGTCGTTCTGTATCGGTTGCCAGCAGCAATTTTACTTGGGAAGACGCTGTCCACATTTCTCTGCCAGAAAGCTTTCAGGATGATCCCTCAGATCTTACCGGCTATTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCAGATATGCAATCCGAATTTCTTCCATTTGTAATCGATGACCAAATTGTTGGTCATGTGCACCACGGCTTTGCTTATCACCTGAAGAAGTATGCGAATGTGTTTACCTTCCCCCGGGATGATTCCGTTCGAATGAATGGTTATGTTGAAAAGGATGGAAAGAAATTTTTGTGGATAGCAAAAAGAAGTCAAATGAAGCAAACTTTTCCTGGAATGCTGGATGAATTAGTAGCTGGAGGGCTGCCTGAGGGCGTTTCTTGCGAGGAGAATCTGATGAAGGAATGTGAAGAGGAAGCAGGCATACCAAGATCAATATCAAATAAAGCCATACCCGTTGGTGCTGTTTCCTATTCAGACATCAAAGATTATAGTTACAAGAGAGATGTTCTATTTTGTTACGATTTAAAGCTTCCTGAAAGTTTTATACCGAAGAATCAAGATGGGGAGGTTGAAGGATTCAGGTTGTTGCCCGTGACAAATGTTGCAAACGTGATTCGGAGGACACAGTTATTCAAGCATAATTGTGCTCTCGTCATCATTGACTTCCTATTTCGACATGGGTACATTAAACCCGAAAGTTTGGGGTACTTGGAGCTACTACAAAGTTTGAAGAGTGGAACCTGCTTCTAA

Protein sequence

MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHHGFAYHLKKYANVFTFPRDDSVRMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQSLKSGTCF

Homology

[Close]

BLAST of Carg12184 vs. NCBI nr
Match: KAG7021837.1 (Nudix hydrolase 20, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 629.8 bits (1623), Expect = 1.2e-176
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 1   MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVA 60
           MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVA
Sbjct: 1   MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVA 60

Query: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120
           SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH
Sbjct: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120

Query: 121 GFAYHLKKYANVFTFPRDDSVRMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLP 180
           GFAYHLKKYANVFTFPRDDSVRMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLP
Sbjct: 121 GFAYHLKKYANVFTFPRDDSVRMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLP 180

Query: 181 EGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFI 240
           EGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFI
Sbjct: 181 EGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFI 240

Query: 241 PKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQS 300
           PKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQS
Sbjct: 241 PKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKPESLGYLELLQS 300

Query: 301 LKSGTCF 308
           LKSGTCF
Sbjct: 301 LKSGTCF 307

[Close]

BLAST of Carg12184 vs. NCBI nr
Match: KAG6587948.1 (Nudix hydrolase 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 598.2 bits (1541), Expect = 4.0e-167
Identity = 307/378 (81.22%), Postives = 307/378 (81.22%), Query Frame = 0


Query: 1   MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVA 60
           MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVA
Sbjct: 1   MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVA 60

Query: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120
           SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH
Sbjct: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120

Query: 121 GFAYHLKKYANVFTFPRDDSVR-------------------------------------- 180
           GFAYHLKKYANVFTFPRDDSVR                                      
Sbjct: 121 GFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIQGIRN 180

Query: 181 ---------------------------------MNGYVEKDGKKFLWIAKRSQMKQTFPG 240
                                            MNGYVEKDGKKFLWIAKRSQMKQTFPG
Sbjct: 181 EMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 240

Query: 241 MLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300
           MLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF
Sbjct: 241 MLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300

Query: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 308
           CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP
Sbjct: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 360

[Close]

BLAST of Carg12184 vs. NCBI nr
Match: XP_023530783.1 (nudix hydrolase 20, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023530784.1 nudix hydrolase 20, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 588.6 bits (1516), Expect = 3.2e-164
Identity = 303/378 (80.16%), Postives = 303/378 (80.16%), Query Frame = 0


Query: 1   MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVA 60
           M TCPRIQPMACKSLASHRFRNS PISSI NASMFSRATYPTASTVHRSKTFACRSVSVA
Sbjct: 1   MVTCPRIQPMACKSLASHRFRNSLPISSISNASMFSRATYPTASTVHRSKTFACRSVSVA 60

Query: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120
           SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH
Sbjct: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120

Query: 121 GFAYHLKKYANVFTFPRDDSVR-------------------------------------- 180
           GFAYHLKKYANVFTFPRDDSVR                                      
Sbjct: 121 GFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPGIRN 180

Query: 181 ---------------------------------MNGYVEKDGKKFLWIAKRSQMKQTFPG 240
                                            MNGYVEKDGKKFLWIAKRSQMKQTFPG
Sbjct: 181 EMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 240

Query: 241 MLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300
           MLDELVAGGLPEGVSC ENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF
Sbjct: 241 MLDELVAGGLPEGVSCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300

Query: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 308
           CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP
Sbjct: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 360

[Close]

BLAST of Carg12184 vs. NCBI nr
Match: XP_022933802.1 (nudix hydrolase 20, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 586.6 bits (1511), Expect = 1.2e-163
Identity = 302/378 (79.89%), Postives = 303/378 (80.16%), Query Frame = 0


Query: 1   MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVA 60
           MATCPRIQPMACKSLASHRFRNSSPISSI NASMFSRA YPTAST+HRSKTFACRSVSVA
Sbjct: 1   MATCPRIQPMACKSLASHRFRNSSPISSISNASMFSRARYPTASTLHRSKTFACRSVSVA 60

Query: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120
           SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH
Sbjct: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120

Query: 121 GFAYHLKKYANVFTFPRDDSVR-------------------------------------- 180
           GFAYHLKKYANVFTFPRDDSVR                                      
Sbjct: 121 GFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPGIRN 180

Query: 181 ---------------------------------MNGYVEKDGKKFLWIAKRSQMKQTFPG 240
                                            MNGYVEKDGKKFLWIAKRSQMKQTFPG
Sbjct: 181 EMYPVTSSFGAPVFFSLERAAATYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 240

Query: 241 MLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300
           MLDELVAGGLPEGVSC ENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF
Sbjct: 241 MLDELVAGGLPEGVSCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300

Query: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 308
           CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP
Sbjct: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 360

[Close]

BLAST of Carg12184 vs. NCBI nr
Match: XP_023002396.1 (nudix hydrolase 20, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 585.5 bits (1508), Expect = 2.7e-163
Identity = 300/378 (79.37%), Postives = 303/378 (80.16%), Query Frame = 0


Query: 1   MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVA 60
           MATCPRIQPMACKSLASH FRNSSPISSI NASMFSRATYPTASTVHRSKTFACRSVS+A
Sbjct: 1   MATCPRIQPMACKSLASHPFRNSSPISSISNASMFSRATYPTASTVHRSKTFACRSVSIA 60

Query: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120
           SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH
Sbjct: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120

Query: 121 GFAYHLKKYANVFTFPRDDSVR-------------------------------------- 180
           GFAYHLKKYANVFTFPRDDSVR                                      
Sbjct: 121 GFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPGVRN 180

Query: 181 ---------------------------------MNGYVEKDGKKFLWIAKRSQMKQTFPG 240
                                            MNGYVEKDGKKFLWIAKRSQMKQTFPG
Sbjct: 181 EMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 240

Query: 241 MLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300
           MLDELVAGGLPEG+SC ENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF
Sbjct: 241 MLDELVAGGLPEGISCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300

Query: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 308
           CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVII+FLFRHGYIKP
Sbjct: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIEFLFRHGYIKP 360

[Close]

BLAST of Carg12184 vs. ExPASy Swiss-Prot
Match: Q8VXZ0 (Nudix hydrolase 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT20 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.3e-80
Identity = 158/363 (43.53%), Postives = 211/363 (58.13%), Query Frame = 0


Query: 27  SSIFNASMFSRATYP---------TASTVHRSKTFACRSVSVASSNFTWEDAVHISLPES 86
           +S+F++   +R   P         + S +  S+  +  +    SS+FTW+D +     E 
Sbjct: 14  TSLFSSHAITRRVLPILRWRSSSMSLSPLRHSRALSAATTVPISSSFTWDDVIETGRAEY 73

Query: 87  FQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHHGFAYHLKKYANVFTFPR 146
              + SDLTG+FEK+  CNRGS+   EF+PFVI++QIVG++H  F  +L+++ ++FTF +
Sbjct: 74  ---NSSDLTGFFEKINRCNRGSEKLGEFIPFVIEEQIVGYIHKRFTEYLREFHDIFTFSQ 133

Query: 147 DDS--------------------------------------------------------- 206
           + S                                                         
Sbjct: 134 NGSCPDRVDGYVTLNLMLQKPEDRTRAVADVIKILGDKGIIPGIRNELYPVKPSFNAPVF 193

Query: 207 -----------------VRMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGV 266
                            V MNGYVE+DG+K LWI KRS  K T+PGMLD LVAGGLP G+
Sbjct: 194 FSLERAAAPYFGIKGYGVHMNGYVERDGQKLLWIGKRSLSKSTYPGMLDHLVAGGLPHGI 253

Query: 267 SCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPKN 307
           SC  NL+KECEEEAGI R+I+++AI VGAVSY DI  Y +KRDVLFCYDL+LPE F+PKN
Sbjct: 254 SCGGNLVKECEEEAGISRAIADRAIAVGAVSYLDIDQYCFKRDVLFCYDLELPEDFVPKN 313

[Close]

BLAST of Carg12184 vs. ExPASy Swiss-Prot
Match: P0C026 (Nudix hydrolase 24, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT24 PE=2 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 2.6e-76
Identity = 146/328 (44.51%), Postives = 194/328 (59.15%), Query Frame = 0


Query: 46  VHRSKTFACRSVSVASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEF 105
           +H S+  +  +V + S +FTW D    S  E    + SDLTG+ EKV  CNRG +   EF
Sbjct: 38  LHISRVLSVETVPL-SPSFTWNDVFENSRKEYVPQNSSDLTGFLEKVDRCNRGLEKLGEF 97

Query: 106 LPFVIDDQIVGHVHHGFAYHLKKYANVFTFP----------------------------- 165
           +PFVI++QIVG++H GF  +L+ + ++FTF                              
Sbjct: 98  IPFVIEEQIVGYIHKGFTKYLRDFNDIFTFSQYGGHVTLNMMLDKPEERTRAVAHVIKIL 157

Query: 166 --------------------------------------RDDSVRMNGYVEKDGKKFLWIA 225
                                                 +  ++ +NGYVE+DG+KFLWI 
Sbjct: 158 GNKGIIPGIRNELYPVKPSFNAPAFFSIERAAAPYFGLKGYAIHVNGYVERDGQKFLWIG 217

Query: 226 KRSQMKQTFPGMLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDI 285
           KRS  K T+PG LD LVAGGLP G+S  ENL+KECEEEAGI + ++++AI VG VSY DI
Sbjct: 218 KRSLAKSTYPGKLDHLVAGGLPHGISVCENLVKECEEEAGISKVLADRAIAVGVVSYMDI 277

Query: 286 KDYSYKRDVLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVII 307
             Y + RDVLFCYDL+LP+ F+P NQDGEV+ FRL+PV  VANV+R+T  FK +C+LVII
Sbjct: 278 DRYCFTRDVLFCYDLELPQDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVII 337

[Close]

BLAST of Carg12184 vs. ExPASy Swiss-Prot
Match: P47173 (Uncharacterized protein YJR142W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YJR142W PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.0e-27
Identity = 74/173 (42.77%), Postives = 100/173 (57.80%), Query Frame = 0


Query: 141 VRMNGYV--EKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGVSCEENLMKECEEEAG 200
           + +NGYV   K  K   W+ +RS+ KQT+P MLD ++AGGL       E ++KE  EEA 
Sbjct: 158 IHINGYVLDPKSKKVQFWVPRRSKTKQTWPLMLDNIIAGGLGYPYGIYETVLKESMEEAN 217

Query: 201 IPRSISNKAI-PVGAVSY----SDIKDYSYKR-------DVLFCYDLKLPESFIPKNQDG 260
           + +S+    I   G+VSY     DI    + +       +V + YDLKL E  IPK  DG
Sbjct: 218 LEKSVIEDNIKATGSVSYLYFTGDISVTKFNKESDFIVGEVQYVYDLKLSEDIIPKPNDG 277

Query: 261 EVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKPES-LGYLELL 299
           EVE F L  +    N +R+ + FK NCALV++DFL RHGYI PE+   YLEL+
Sbjct: 278 EVESFNLFSLQETINALRKKE-FKPNCALVMVDFLIRHGYITPENEPNYLELV 329

[Close]

BLAST of Carg12184 vs. ExPASy Swiss-Prot
Match: P41888 (Thiamine pyrophosphokinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tnr3 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.5e-23
Identity = 61/148 (41.22%), Postives = 89/148 (60.14%), Query Frame = 0


Query: 155 LWIAKRSQMKQTFPGMLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVS 214
           +W+ +RS  KQT+P  LD  VAGG+  G S    ++KE  EEA +  S  N  IP G VS
Sbjct: 153 IWVPRRSPTKQTWPNYLDNSVAGGIAHGDSVIGTMIKEFSEEANLDVSSMN-LIPCGTVS 212

Query: 215 YSDI-KDYSYKRDVLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNC 274
           Y  + K +  + ++ + +DL + +  IP+  DGEV GF LLP+  V + +   + FK NC
Sbjct: 213 YIKMEKRHWIQPELQYVFDLPVDDLVIPRINDGEVAGFSLLPLNQVLHEL-ELKSFKPNC 272

Query: 275 ALVIIDFLFRHGYIKPESLGYLELLQSL 302
           ALV++DFL RHG I P+   YL+ L+ +
Sbjct: 273 ALVLLDFLIRHGIITPQHPQYLQTLERI 298

[Close]

BLAST of Carg12184 vs. ExPASy TrEMBL
Match: A0A6J1F0U6 (nudix hydrolase 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111441109 PE=4 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 5.8e-164
Identity = 302/378 (79.89%), Postives = 303/378 (80.16%), Query Frame = 0


Query: 1   MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVA 60
           MATCPRIQPMACKSLASHRFRNSSPISSI NASMFSRA YPTAST+HRSKTFACRSVSVA
Sbjct: 1   MATCPRIQPMACKSLASHRFRNSSPISSISNASMFSRARYPTASTLHRSKTFACRSVSVA 60

Query: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120
           SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH
Sbjct: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120

Query: 121 GFAYHLKKYANVFTFPRDDSVR-------------------------------------- 180
           GFAYHLKKYANVFTFPRDDSVR                                      
Sbjct: 121 GFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPGIRN 180

Query: 181 ---------------------------------MNGYVEKDGKKFLWIAKRSQMKQTFPG 240
                                            MNGYVEKDGKKFLWIAKRSQMKQTFPG
Sbjct: 181 EMYPVTSSFGAPVFFSLERAAATYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 240

Query: 241 MLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300
           MLDELVAGGLPEGVSC ENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF
Sbjct: 241 MLDELVAGGLPEGVSCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300

Query: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 308
           CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP
Sbjct: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 360

[Close]

BLAST of Carg12184 vs. ExPASy TrEMBL
Match: A0A6J1KL70 (nudix hydrolase 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111496248 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.3e-163
Identity = 300/378 (79.37%), Postives = 303/378 (80.16%), Query Frame = 0


Query: 1   MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVA 60
           MATCPRIQPMACKSLASH FRNSSPISSI NASMFSRATYPTASTVHRSKTFACRSVS+A
Sbjct: 1   MATCPRIQPMACKSLASHPFRNSSPISSISNASMFSRATYPTASTVHRSKTFACRSVSIA 60

Query: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120
           SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH
Sbjct: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120

Query: 121 GFAYHLKKYANVFTFPRDDSVR-------------------------------------- 180
           GFAYHLKKYANVFTFPRDDSVR                                      
Sbjct: 121 GFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPGVRN 180

Query: 181 ---------------------------------MNGYVEKDGKKFLWIAKRSQMKQTFPG 240
                                            MNGYVEKDGKKFLWIAKRSQMKQTFPG
Sbjct: 181 EMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 240

Query: 241 MLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300
           MLDELVAGGLPEG+SC ENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF
Sbjct: 241 MLDELVAGGLPEGISCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300

Query: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 308
           CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVII+FLFRHGYIKP
Sbjct: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIEFLFRHGYIKP 360

[Close]

BLAST of Carg12184 vs. ExPASy TrEMBL
Match: A0A6J1CUZ7 (nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111014513 PE=4 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.8e-136
Identity = 262/373 (70.24%), Postives = 276/373 (73.99%), Query Frame = 0


Query: 10  MACKSLASHRFRNSSPISSIFNASMFSRATYPTAS----TVHRSKTFACRSVSVASSNFT 69
           MACKSLA HRF N   IS I +ASMF R  +PT S    TVHRS TFA  SVSVASS+FT
Sbjct: 1   MACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSVSVASSSFT 60

Query: 70  WEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHHGFAYH 129
           WEDA+HISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVID+QI+G+VHHGFA H
Sbjct: 61  WEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGYVHHGFAEH 120

Query: 130 LKKYANVFTFPRDDS--------------------------------------------- 189
           LKKYANVFTFP+DDS                                             
Sbjct: 121 LKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPGTRNELYPV 180

Query: 190 --------------------------VRMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDEL 249
                                     V+MNGYVE+DG+KFLWIAKRSQMKQTFPGMLDEL
Sbjct: 181 TSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVERDGQKFLWIAKRSQMKQTFPGMLDEL 240

Query: 250 VAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLFCYDLK 308
           VAGGLP+GV C ENL KECEEEAGIPRSISNKAI VGAVSYSDIKDYSYKRDVLFCYDLK
Sbjct: 241 VAGGLPQGVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRDVLFCYDLK 300

[Close]

BLAST of Carg12184 vs. ExPASy TrEMBL
Match: A0A6J1CSY3 (nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111014470 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.5e-127
Identity = 252/379 (66.49%), Postives = 271/379 (71.50%), Query Frame = 0


Query: 7   IQPMACKSLA--SHRFRNSSPISSIFNASMFSRATYPTAST-----VHRSKTFACRSVSV 66
           IQ MACKSLA  SHRFRN S ISS  NAS  SR TYP AST     VHRS + A RSVSV
Sbjct: 14  IQFMACKSLASHSHRFRNFSTISSNCNASTISRTTYPMASTFRPTVVHRSISVASRSVSV 73

Query: 67  ASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVH 126
           ASS FTW+D + ISLPE +QDDPSDLTGYFEKVKLCNRGSDMQSEFLPFV+DDQIVG+VH
Sbjct: 74  ASS-FTWDDVLRISLPEIYQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVVDDQIVGYVH 133

Query: 127 HGFAYHLKKYANVFTFPRDDSVR------------------------------------- 186
           HGFA HLK+YANVFTF  DDSV+                                     
Sbjct: 134 HGFAEHLKQYANVFTFSGDDSVQFRAYLTLHESLKTPEDRTQAVGDVVKCLGEEIIPGTR 193

Query: 187 ----------------------------------MNGYVEKDGKKFLWIAKRSQMKQTFP 246
                                             MNGYVEK+GKKFLW+AKRSQ KQTFP
Sbjct: 194 NELYPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKQTFP 253

Query: 247 GMLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVL 306
           GMLD LVAGGLP+G+ C ENLMKECEEEA IPRS+SN+AIPVGAVSYSDIK ++YKRDV 
Sbjct: 254 GMLDHLVAGGLPQGIPCVENLMKECEEEACIPRSLSNEAIPVGAVSYSDIKGHTYKRDVQ 313

Query: 307 FCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIK 308
           FCYDLKLPESFIP+NQDGEVEGF LLPVTNVANVIRRTQLFK NC+LVIIDFLFRHGYI 
Sbjct: 314 FCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPNCSLVIIDFLFRHGYIY 373

[Close]

BLAST of Carg12184 vs. ExPASy TrEMBL
Match: A0A6J1HA39 (LOW QUALITY PROTEIN: nudix hydrolase 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111461857 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 7.0e-125
Identity = 236/372 (63.44%), Postives = 261/372 (70.16%), Query Frame = 0


Query: 7   IQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVASSNFTW 66
           IQ MACKSL +HRF N S +SS++NAS  SR TYP  ST   + T   RS+SVA+ +F+W
Sbjct: 14  IQFMACKSLVAHRFHNLSIVSSLYNASKISRTTYPMLSTFRPAVT---RSISVAAGSFSW 73

Query: 67  EDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHHGFAYHL 126
           +D VHISLP SFQDDPSDLTGYFEKVKLCNRGSD QSEFLPFVID+QIVG+VHHGFA HL
Sbjct: 74  DDVVHISLPSSFQDDPSDLTGYFEKVKLCNRGSDTQSEFLPFVIDNQIVGYVHHGFAKHL 133

Query: 127 KKYANVFTFPRDD----------------------------------------------- 186
           K+Y NVF FPRDD                                               
Sbjct: 134 KQYPNVFIFPRDDCGQLGAYLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGTRNELYPVT 193

Query: 187 ------------------------SVRMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELV 246
                                    V MNGYVE++G+KFLW+AKRSQ K TFPGMLD LV
Sbjct: 194 SSFGASSFFSLERAAAPYFGIKVYGVHMNGYVEREGEKFLWVAKRSQTKPTFPGMLDHLV 253

Query: 247 AGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLFCYDLKL 306
           AGGLP+G+ C EN+MKECEEEAGIPRSIS  AIPVGA+SY+DIK +SYKRDV FCYDLKL
Sbjct: 254 AGGLPQGIPCGENVMKECEEEAGIPRSISKGAIPVGAISYTDIKGFSYKRDVQFCYDLKL 313

Query: 307 PESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKPESLGYL 308
           PESFIP+NQDGEVEGF LLPVTNVANVIRRTQLFK NC LVIIDFLFRHGYI PES GYL
Sbjct: 314 PESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPNCTLVIIDFLFRHGYINPESSGYL 373

[Close]

BLAST of Carg12184 vs. TAIR 10
Match: AT5G19460.1 (nudix hydrolase homolog 20 )

HSP 1 Score: 301.2 bits (770), Expect = 9.4e-82
Identity = 158/363 (43.53%), Postives = 211/363 (58.13%), Query Frame = 0


Query: 27  SSIFNASMFSRATYP---------TASTVHRSKTFACRSVSVASSNFTWEDAVHISLPES 86
           +S+F++   +R   P         + S +  S+  +  +    SS+FTW+D +     E 
Sbjct: 14  TSLFSSHAITRRVLPILRWRSSSMSLSPLRHSRALSAATTVPISSSFTWDDVIETGRAEY 73

Query: 87  FQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHHGFAYHLKKYANVFTFPR 146
              + SDLTG+FEK+  CNRGS+   EF+PFVI++QIVG++H  F  +L+++ ++FTF +
Sbjct: 74  ---NSSDLTGFFEKINRCNRGSEKLGEFIPFVIEEQIVGYIHKRFTEYLREFHDIFTFSQ 133

Query: 147 DDS--------------------------------------------------------- 206
           + S                                                         
Sbjct: 134 NGSCPDRVDGYVTLNLMLQKPEDRTRAVADVIKILGDKGIIPGIRNELYPVKPSFNAPVF 193

Query: 207 -----------------VRMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPEGV 266
                            V MNGYVE+DG+K LWI KRS  K T+PGMLD LVAGGLP G+
Sbjct: 194 FSLERAAAPYFGIKGYGVHMNGYVERDGQKLLWIGKRSLSKSTYPGMLDHLVAGGLPHGI 253

Query: 267 SCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLFCYDLKLPESFIPKN 307
           SC  NL+KECEEEAGI R+I+++AI VGAVSY DI  Y +KRDVLFCYDL+LPE F+PKN
Sbjct: 254 SCGGNLVKECEEEAGISRAIADRAIAVGAVSYLDIDQYCFKRDVLFCYDLELPEDFVPKN 313

[Close]

BLAST of Carg12184 vs. TAIR 10
Match: AT5G19470.1 (nudix hydrolase homolog 24 )

HSP 1 Score: 287.0 bits (733), Expect = 1.8e-77
Identity = 146/328 (44.51%), Postives = 194/328 (59.15%), Query Frame = 0


Query: 46  VHRSKTFACRSVSVASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEF 105
           +H S+  +  +V + S +FTW D    S  E    + SDLTG+ EKV  CNRG +   EF
Sbjct: 38  LHISRVLSVETVPL-SPSFTWNDVFENSRKEYVPQNSSDLTGFLEKVDRCNRGLEKLGEF 97

Query: 106 LPFVIDDQIVGHVHHGFAYHLKKYANVFTFP----------------------------- 165
           +PFVI++QIVG++H GF  +L+ + ++FTF                              
Sbjct: 98  IPFVIEEQIVGYIHKGFTKYLRDFNDIFTFSQYGGHVTLNMMLDKPEERTRAVAHVIKIL 157

Query: 166 --------------------------------------RDDSVRMNGYVEKDGKKFLWIA 225
                                                 +  ++ +NGYVE+DG+KFLWI 
Sbjct: 158 GNKGIIPGIRNELYPVKPSFNAPAFFSIERAAAPYFGLKGYAIHVNGYVERDGQKFLWIG 217

Query: 226 KRSQMKQTFPGMLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDI 285
           KRS  K T+PG LD LVAGGLP G+S  ENL+KECEEEAGI + ++++AI VG VSY DI
Sbjct: 218 KRSLAKSTYPGKLDHLVAGGLPHGISVCENLVKECEEEAGISKVLADRAIAVGVVSYMDI 277

Query: 286 KDYSYKRDVLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVII 307
             Y + RDVLFCYDL+LP+ F+P NQDGEV+ FRL+PV  VANV+R+T  FK +C+LVII
Sbjct: 278 DRYCFTRDVLFCYDLELPQDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVII 337

[Close]

BLAST of Carg12184 vs. TAIR 10
Match: AT5G19470.2 (nudix hydrolase homolog 24 )

HSP 1 Score: 272.3 bits (695), Expect = 4.7e-73
Identity = 142/328 (43.29%), Postives = 189/328 (57.62%), Query Frame = 0


Query: 46  VHRSKTFACRSVSVASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEF 105
           +H S+  +  +V + S +FTW D    S  E    + SDLTG+ EKV  CNRG +   EF
Sbjct: 38  LHISRVLSVETVPL-SPSFTWNDVFENSRKEYVPQNSSDLTGFLEKVDRCNRGLEKLGEF 97

Query: 106 LPFVIDDQIVGHVHHGFAYHLKKYANVFTFP----------------------------- 165
           +PFVI++QIVG++H GF  +L+ + ++FTF                              
Sbjct: 98  IPFVIEEQIVGYIHKGFTKYLRDFNDIFTFSQYGGHVTLNMMLDKPEERTRAVAHVIKIL 157

Query: 166 --------------------------------------RDDSVRMNGYVEKDGKKFLWIA 225
                                                 +  ++ +NGYVE+DG+KFLWI 
Sbjct: 158 GNKGIIPGIRNELYPVKPSFNAPAFFSIERAAAPYFGLKGYAIHVNGYVERDGQKFLWIG 217

Query: 226 KRSQMKQTFPGMLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDI 285
           KRS  K T+PG LD LVAGGL        NL+KECEEEAGI + ++++AI VG VSY DI
Sbjct: 218 KRSLAKSTYPGKLDHLVAGGL--------NLVKECEEEAGISKVLADRAIAVGVVSYMDI 277

Query: 286 KDYSYKRDVLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVII 307
             Y + RDVLFCYDL+LP+ F+P NQDGEV+ FRL+PV  VANV+R+T  FK +C+LVII
Sbjct: 278 DRYCFTRDVLFCYDLELPQDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVII 337

The following BLAST results are available for this feature:

BLAST of Carg12184 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021837.1	1.2e-176	100.00	Nudix hydrolase 20, chloroplastic, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6587948.1	4.0e-167	81.22	Nudix hydrolase 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023530783.1	3.2e-164	80.16	nudix hydrolase 20, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_02353078...	[more]
XP_022933802.1	1.2e-163	79.89	nudix hydrolase 20, chloroplastic-like [Cucurbita moschata]	[more]
XP_023002396.1	2.7e-163	79.37	nudix hydrolase 20, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of Carg12184 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VXZ0	1.3e-80	43.53	Nudix hydrolase 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT20 PE=2...	[more]
P0C026	2.6e-76	44.51	Nudix hydrolase 24, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT24 PE=2...	[more]
P47173	1.0e-27	42.77	Uncharacterized protein YJR142W OS=Saccharomyces cerevisiae (strain ATCC 204508 ...	[more]
P41888	1.5e-23	41.22	Thiamine pyrophosphokinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843...	[more]

BLAST of Carg12184 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F0U6	5.8e-164	79.89	nudix hydrolase 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1KL70	1.3e-163	79.37	nudix hydrolase 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114962...	[more]
A0A6J1CUZ7	1.8e-136	70.24	nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1CSY3	1.5e-127	66.49	nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1HA39	7.0e-125	63.44	LOW QUALITY PROTEIN: nudix hydrolase 20, chloroplastic-like OS=Cucurbita moschat...	[more]

BLAST of Carg12184 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19460.1	9.4e-82	43.53	nudix hydrolase homolog 20	[more]
AT5G19470.1	1.8e-77	44.51	nudix hydrolase homolog 24	[more]
AT5G19470.2	4.7e-73	43.29	nudix hydrolase homolog 24	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000086	NUDIX hydrolase domain	PFAM	PF00293	NUDIX	coord: 150..259 e-value: 1.2E-9 score: 38.3
IPR000086	NUDIX hydrolase domain	PROSITE	PS51462	NUDIX	coord: 136..279 score: 11.371316
None	No IPR available	GENE3D	3.90.79.10	Nucleoside Triphosphate Pyrophosphohydrolase	coord: 126..303 e-value: 5.0E-61 score: 207.6
None	No IPR available	PANTHER	PTHR13622:SF10	SI:DKEY-6N6.2	coord: 63..136 coord: 141..304
None	No IPR available	PANTHER	PTHR13622	THIAMIN PYROPHOSPHOKINASE	coord: 141..304
None	No IPR available	PANTHER	PTHR13622	THIAMIN PYROPHOSPHOKINASE	coord: 63..136
IPR031804	Domain of unknown function DUF4743	PFAM	PF15916	DUF4743	coord: 88..145 e-value: 1.3E-11 score: 44.5
IPR015797	NUDIX hydrolase-like domain superfamily	SUPERFAMILY	55811	Nudix	coord: 126..286

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12184-RA	Carg12184-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity