Carg12146 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12146
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Pentatricopeptide repeat-containing protein
Location	Carg_Chr11: 2458184 .. 2459679 (-)
RNA-Seq Expression	Carg12146
Synteny	Carg12146

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTCGACGGCTCTCGATGAGTCTGTCAGCGGTTATGCCATTAGTTTTCTTTGTCCGAATGATTCATTCGGGAGTTAAGCCGGATTCTTATACTTTTCCTTTTCTATTCAAGTCTTGCGCGAAGCTAGCCTCTGCCCATGGAGGGAAACAGATTCGTGCGTATGTCTAGAAGCTTGGGTTTGGTTTGATGTGTATATTCATAATTCGCTCATCATCAATGCCCATTTGGTGTTTGATCAAAGTAATTTAAGGGATGCAATTTCTTTCACTGCACCAATTGCGGGTTACGCTATGTGGGGTAATACGGATCGCGCACGGAAAGTGTTTGGTGAAATGCCTGTTAGAGACGTGGTATCTTGGAATGCTATGGTTGCTGGCTATGGGCAAACTAGTCGGTCCAAGAGGGATCGTTAATGTTTGAAGAAACGAGGAAGGCAAATGTTACCCGTCTCTGTTCTTTCTGCTTGTGCTAAGTCAAATGCTCTAGATTTTGGAAACTCGACACGCTTTTGGATTGAAGATTTTGGGCTTCATTCGAATCTTAAGCTTGTTAATGCACTTATTGTGATCTTCAGACTGCTCGTGAGTTGTTTGACGATATGCCTGAAAGGGATGTGGTCTCATGGACTGTAATGATCAGAGTTTACACTCACATGAGCAGCTATACATGTTTATATAATCATAAACTTCGACTCTGCCAGCACCTCTCTTTGGACGAGTCTGGTCGATATGTATGCTAAATGCGGCAAGATGGCAGGCTTTCCGATCATACGACACTTCACTTAATTTTGCATGTAAACTCGGTTGCCGATCATGTAATTGTAACCTGCTATAAATTTTTATTTAAAAAAATATATCCCAGTATAGTTCCCAACAGTAAACTCTTCCCCCACAGTGGAATTTGAGCCTGTGGCTGATGAACAAACTACATCTTTGTGCCGAGGGAAGCTGAGGGAAGGTAAAGCTGGTATGCCCTTTGTGCCGAGGTAAGGTATAAAAGTGGGTTGTTGATGAGCATGCTCGTCAGTTCATGAATGCAAAATCAAGAAGTTGTTCTTGTGAGACATGTAACTTCAGCGGAACCTATACAGATCTTAGGAAGTACGCAAGGAAGGAGTGTCCTCTTGTTCGTCCATCAGAAGTCGACCCAGAACGACAGCATAATTGGAGACGAATTGAGCTAGCACACTACAACCATCATTTGGAGAGGATAGGGTTGATGGTGATAGTATTTTGCCCATTGATAATGGGGGCTGGCTCACAGTTTTTTTTTCCTTATTCGAGTTTTTAGACCTGAATCCGGTAGAAGGAGCAGCAGTTGGTCTAATGTGTCTAGAGCTAGAGAGAAACCAAGTTATAGGAGGCGAGAGTTATGATTGGGAAATAGGATCATCTTCCAGAGATGAAGATAATGACTCTTCAGACGATGGATCAGGATCGTCGAGGCATCAAGATCGCACACAGCGACAGACAACACCTGATAATCCACCTTGA

mRNA sequence

ATGATTCGACGGCTCTCGATGAGTCTGTCAGCGGTTATGCCATTAGTTTTCTTTGTCCGAATGATTCATTCGGGAGTTAAGCCGGATTCTTATACTTTTCCTTTTCTATTCAAGTCTTGCGCGAAGCTAGCCTCTGCCCATGGAGGGAAACAGATTCGTGCGGATGCAATTTCTTTCACTGCACCAATTGCGGGTTACGCTATGTGGGGTAATACGGATCGCGCACGGAAAGTGTTTGGTGAAATGCCTGTTAGAGACGTGACTGCTCGTGAGTTGTTTGACGATATGCCTGAAAGGGATGTGGTCTCATGGACTGTAATGATCAGAGTTTACACTCACATGAGCAGCTATACATTGGAATTTGAGCCTGTGGCTGATGAACAAACTACATCTTTGTGCCGAGGGAAGCTGAGGGAAGGTAAAGCTGACCTGAATCCGGTAGAAGGAGCAGCAGTTGGTCTAATGTGTCTAGAGCTAGAGAGAAACCAAGTTATAGGAGGCGAGAGTTATGATTGGGAAATAGGATCATCTTCCAGAGATGAAGATAATGACTCTTCAGACGATGGATCAGGATCGTCGAGGCATCAAGATCGCACACAGCGACAGACAACACCTGATAATCCACCTTGA

Coding sequence (CDS)

ATGATTCGACGGCTCTCGATGAGTCTGTCAGCGGTTATGCCATTAGTTTTCTTTGTCCGAATGATTCATTCGGGAGTTAAGCCGGATTCTTATACTTTTCCTTTTCTATTCAAGTCTTGCGCGAAGCTAGCCTCTGCCCATGGAGGGAAACAGATTCGTGCGGATGCAATTTCTTTCACTGCACCAATTGCGGGTTACGCTATGTGGGGTAATACGGATCGCGCACGGAAAGTGTTTGGTGAAATGCCTGTTAGAGACGTGACTGCTCGTGAGTTGTTTGACGATATGCCTGAAAGGGATGTGGTCTCATGGACTGTAATGATCAGAGTTTACACTCACATGAGCAGCTATACATTGGAATTTGAGCCTGTGGCTGATGAACAAACTACATCTTTGTGCCGAGGGAAGCTGAGGGAAGGTAAAGCTGACCTGAATCCGGTAGAAGGAGCAGCAGTTGGTCTAATGTGTCTAGAGCTAGAGAGAAACCAAGTTATAGGAGGCGAGAGTTATGATTGGGAAATAGGATCATCTTCCAGAGATGAAGATAATGACTCTTCAGACGATGGATCAGGATCGTCGAGGCATCAAGATCGCACACAGCGACAGACAACACCTGATAATCCACCTTGA

Protein sequence

MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRADAISFTAPIAGYAMWGNTDRARKVFGEMPVRDVTARELFDDMPERDVVSWTVMIRVYTHMSSYTLEFEPVADEQTTSLCRGKLREGKADLNPVEGAAVGLMCLELERNQVIGGESYDWEIGSSSRDEDNDSSDDGSGSSRHQDRTQRQTTPDNPP

Homology

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BLAST of Carg12146 vs. NCBI nr
Match: KAG7021800.1 (Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 414.8 bits (1065), Expect = 4.3e-112
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRADAISFT 60
           MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRADAISFT
Sbjct: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRADAISFT 60

Query: 61  APIAGYAMWGNTDRARKVFGEMPVRDVTARELFDDMPERDVVSWTVMIRVYTHMSSYTLE 120
           APIAGYAMWGNTDRARKVFGEMPVRDVTARELFDDMPERDVVSWTVMIRVYTHMSSYTLE
Sbjct: 61  APIAGYAMWGNTDRARKVFGEMPVRDVTARELFDDMPERDVVSWTVMIRVYTHMSSYTLE 120

Query: 121 FEPVADEQTTSLCRGKLREGKADLNPVEGAAVGLMCLELERNQVIGGESYDWEIGSSSRD 180
           FEPVADEQTTSLCRGKLREGKADLNPVEGAAVGLMCLELERNQVIGGESYDWEIGSSSRD
Sbjct: 121 FEPVADEQTTSLCRGKLREGKADLNPVEGAAVGLMCLELERNQVIGGESYDWEIGSSSRD 180

Query: 181 EDNDSSDDGSGSSRHQDRTQRQTTPDNPP 210
           EDNDSSDDGSGSSRHQDRTQRQTTPDNPP
Sbjct: 181 EDNDSSDDGSGSSRHQDRTQRQTTPDNPP 209

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BLAST of Carg12146 vs. NCBI nr
Match: KAG6587914.1 (hypothetical protein SDJN03_16479, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 185.7 bits (470), Expect = 4.2e-43
Identity = 92/106 (86.79%), Postives = 97/106 (91.51%), Query Frame = 0


Query: 104 WTVMIRVYTHMSSYTLEFEPVADEQTTSLCRGKLREGKADLNPVEGAAVGLMCLELERNQ 163
           WT ++ +Y       +EFEPVADEQTTSLCRGKLREGKADLNPVEGAAVGLMCLELERNQ
Sbjct: 68  WTSLVDMYAKCGKMAVEFEPVADEQTTSLCRGKLREGKADLNPVEGAAVGLMCLELERNQ 127

Query: 164 VIGGESYDWEIGSSSRDEDNDSSDDGSGSSRHQDRTQRQTTPDNPP 210
           VIGG+SYDWEIGSSSRDEDNDSSDDGSGSSRHQDRTQRQTTPDNPP
Sbjct: 128 VIGGKSYDWEIGSSSRDEDNDSSDDGSGSSRHQDRTQRQTTPDNPP 173

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BLAST of Carg12146 vs. NCBI nr
Match: KAG6587915.1 (Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 173.7 bits (439), Expect = 1.7e-39
Identity = 92/115 (80.00%), Postives = 93/115 (80.87%), Query Frame = 0


Query: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRADAISFT 60
           MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKS AKLASAHGGKQIRADAISFT
Sbjct: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSWAKLASAHGGKQIRADAISFT 60

Query: 61  APIAGYAMWGNTDRARKVFGEMPVRDVTARELFDDMPERDVVSWTVMIRVYTHMS 116
           APIAGYAMWGNTDRARKVFGEMPV              RDVVSW  M+  Y   S
Sbjct: 61  APIAGYAMWGNTDRARKVFGEMPV--------------RDVVSWNAMVAGYGQTS 101

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BLAST of Carg12146 vs. NCBI nr
Match: XP_038878535.1 (pentatricopeptide repeat-containing protein At1g08070, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 132.1 bits (331), Expect = 5.5e-27
Identity = 95/244 (38.93%), Postives = 102/244 (41.80%), Query Frame = 0


Query: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA------ 60
           MIR LSMSLS V+ LVFFVRMI+SGV+P+SYTFPFL KSCAKLASAH GKQI A      
Sbjct: 100 MIRGLSMSLSPVLALVFFVRMIYSGVEPNSYTFPFLLKSCAKLASAHEGKQIHAHLLKLG 159

Query: 61  ----------------------------------DAISFTAPIAGYAMWGNTDRARKVFG 118
                                             DAISFTA IAGYA+WG  D ARK+F 
Sbjct: 160 FQSDVFIHTSLINMYAQSGEMNNAQLVFDQSNFRDAISFTALIAGYALWGYMDHARKLFD 219

[Close]

BLAST of Carg12146 vs. NCBI nr
Match: KAG6589865.1 (Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 129.4 bits (324), Expect = 3.6e-26
Identity = 97/267 (36.33%), Postives = 109/267 (40.82%), Query Frame = 0


Query: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA------ 60
           MIR LS+SLS V+ LVFFVRMIH+GV+P+SYTFPFL KSCAKLASA  GKQI A      
Sbjct: 97  MIRGLSISLSPVLALVFFVRMIHAGVEPNSYTFPFLLKSCAKLASAREGKQIHAHVLKLG 156

Query: 61  ----------------------------------DAISFTAPIAGYAMWGNTDRARKVFG 120
                                             DAISFTA IAGY +WG  DRARK+F 
Sbjct: 157 FVSDVFIHTSLINMYAQSGEINYAQLVFDQSNFRDAISFTALIAGYVLWGYMDRARKLFD 216

Query: 121 EMPVRDV----------------------------------------------------- 141
           EMPVRDV                                                     
Sbjct: 217 EMPVRDVVSWNAMIAGYAQTGRSKEALLLFEEMRKANVPPNESTIVSVLSACAQSNALDL 276

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BLAST of Carg12146 vs. ExPASy Swiss-Prot
Match: Q9LN01 (Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H12 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 5.6e-14
Identity = 54/158 (34.18%), Postives = 74/158 (46.84%), Query Frame = 0


Query: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA------ 60
           M R  ++S   V  L  +V MI  G+ P+SYTFPF+ KSCAK  +   G+QI        
Sbjct: 105 MFRGHALSSDPVSALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQIHGHVLKLG 164

Query: 61  ---DAISFTAPIAGYAMWGNTDRARKVFGEMPVRDV-----------------TARELFD 120
              D    T+ I+ Y   G  + A KVF + P RDV                  A++LFD
Sbjct: 165 CDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGYIENAQKLFD 224

Query: 121 DMPERDVVSWTVMIRVYTHMSSYTLEFEPVADEQTTSL 133
           ++P +DVVSW  MI  Y    +Y    E   D   T++
Sbjct: 225 EIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNV 262

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BLAST of Carg12146 vs. ExPASy Swiss-Prot
Match: Q9LUJ2 (Pentatricopeptide repeat-containing protein At3g22690 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H56 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.9e-10
Identity = 46/129 (35.66%), Postives = 60/129 (46.51%), Query Frame = 0


Query: 15  LVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA---------DAISFTAPIAG 74
           ++ F+RM++SG+ PD YTFPF   +CAK  +   G QI           D     + +  
Sbjct: 119 ILLFLRMMNSGISPDKYTFPFGLSACAKSRAKGNGIQIHGLIVKMGYAKDLFVQNSLVHF 178

Query: 75  YAMWGNTDRARKVFGEMPVRDVTARELFDDMPERDVVSWTVMIRVYTH----MSSYTLEF 131
           YA  G  D ARKV              FD+M ER+VVSWT MI  Y        +  L F
Sbjct: 179 YAECGELDSARKV--------------FDEMSERNVVSWTSMICGYARRDFAKDAVDLFF 233

[Close]

BLAST of Carg12146 vs. ExPASy Swiss-Prot
Match: O49399 (Pentatricopeptide repeat-containing protein At4g18840 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E101 PE=3 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 52/189 (27.51%), Postives = 74/189 (39.15%), Query Frame = 0


Query: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA------ 60
           +IR  + S +  + L  F  M+   V PD Y+F F+ K+CA       G+QI        
Sbjct: 111 VIRAYANSSTPEVALTVFREMLLGPVFPDKYSFTFVLKACAAFCGFEEGRQIHGLFIKSG 170

Query: 61  ----------------------------------DAISFTAPIAGYAMWGNTDRARKVFG 120
                                             DA+S+ + ++ Y   G  D AR +F 
Sbjct: 171 LVTDVFVENTLVNVYGRSGYFEIARKVLDRMPVRDAVSWNSLLSAYLEKGLVDEARALFD 230

Query: 121 EMPVRDV-----------------TARELFDDMPERDVVSWTVMIRVYTHMSSY--TLE- 130
           EM  R+V                  A+E+FD MP RDVVSW  M+  Y H+  Y   LE 
Sbjct: 231 EMEERNVESWNFMISGYAAAGLVKEAKEVFDSMPVRDVVSWNAMVTAYAHVGCYNEVLEV 290

[Close]

BLAST of Carg12146 vs. ExPASy Swiss-Prot
Match: Q9FLS9 (Pentatricopeptide repeat-containing protein At5g61800 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E8 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.4e-09
Identity = 46/129 (35.66%), Postives = 59/129 (45.74%), Query Frame = 0


Query: 17  FFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGG----KQIRADAISF---------TAPI 76
           FFV M    V PD +TFPF+FK+CA  A  +G     K +   A+ F            I
Sbjct: 102 FFVEMRRRSVPPDFHTFPFVFKACA--AKKNGDLTLVKTLHCQALRFGLLSDLFTLNTLI 161

Query: 77  AGYAMWGNTDRARKVFGEMPVRDVT-----------------ARELFDDMPERDVVSWTV 116
             Y++    D A ++F E P RDV                  ARELFD MP RD+VSW  
Sbjct: 162 RVYSLIAPIDSALQLFDENPQRDVVTYNVLIDGLVKAREIVRARELFDSMPLRDLVSWNS 221

[Close]

BLAST of Carg12146 vs. ExPASy Swiss-Prot
Match: Q9SIL5 (Pentatricopeptide repeat-containing protein At2g20540 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E78 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.2e-09
Identity = 42/116 (36.21%), Postives = 55/116 (47.41%), Query Frame = 0


Query: 28  PDSYTFPFLFKSCAKLASAHGGKQI---------RADAISFTAPIAGYAMWGNTDRARKV 87
           PD +TFPF+FKSCA L S + GKQ+         R   ++  A I  Y  + +   A KV
Sbjct: 107 PDRFTFPFMFKSCASLGSCYLGKQVHGHLCKFGPRFHVVTENALIDMYMKFDDLVDAHKV 166

Query: 88  FGEMPVRDV-----------------TARELFDDMPERDVVSWTVMIRVYTHMSSY 118
           F EM  RDV                  A+ LF  M ++ +VSWT MI  YT +  Y
Sbjct: 167 FDEMYERDVISWNSLLSGYARLGQMKKAKGLFHLMLDKTIVSWTAMISGYTGIGCY 222

[Close]

BLAST of Carg12146 vs. ExPASy TrEMBL
Match: A0A5A7TTJ1 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold64G00470 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 3.9e-26
Identity = 93/244 (38.11%), Postives = 102/244 (41.80%), Query Frame = 0


Query: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA------ 60
           MIR LSMSLS V+ LVFFVRMI+SGV+P+SYTFPFL KSCAKLASA  GKQI A      
Sbjct: 100 MIRGLSMSLSPVLALVFFVRMIYSGVEPNSYTFPFLLKSCAKLASAREGKQIHAHVLKLG 159

Query: 61  ----------------------------------DAISFTAPIAGYAMWGNTDRARKVFG 118
                                             DAISFTA IAGYA+WG  DRAR++F 
Sbjct: 160 FVSDVFIHTSLINMYAQSGEMNNAQLIFDQSNFRDAISFTALIAGYALWGYMDRARQLFD 219

[Close]

BLAST of Carg12146 vs. ExPASy TrEMBL
Match: A0A1S3CMX0 (pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502738 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 3.9e-26
Identity = 93/244 (38.11%), Postives = 102/244 (41.80%), Query Frame = 0


Query: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA------ 60
           MIR LSMSLS V+ LVFFVRMI+SGV+P+SYTFPFL KSCAKLASA  GKQI A      
Sbjct: 100 MIRGLSMSLSPVLALVFFVRMIYSGVEPNSYTFPFLLKSCAKLASAREGKQIHAHVLKLG 159

Query: 61  ----------------------------------DAISFTAPIAGYAMWGNTDRARKVFG 118
                                             DAISFTA IAGYA+WG  DRAR++F 
Sbjct: 160 FVSDVFIHTSLINMYAQSGEMNNAQLIFDQSNFRDAISFTALIAGYALWGYMDRARQLFD 219

[Close]

BLAST of Carg12146 vs. ExPASy TrEMBL
Match: A0A6J1HAU9 (pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111461670 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 6.6e-26
Identity = 92/244 (37.70%), Postives = 101/244 (41.39%), Query Frame = 0


Query: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA------ 60
           MIR LS+SLS V+ LVFFVRMIH+GV+P+SYTFPFL KSCAKLASA  GKQI A      
Sbjct: 97  MIRGLSISLSPVLALVFFVRMIHAGVEPNSYTFPFLLKSCAKLASAREGKQIHAHVLKLG 156

Query: 61  ----------------------------------DAISFTAPIAGYAMWGNTDRARKVFG 118
                                             DAISFTA IAGY +WG  DRARK+F 
Sbjct: 157 FVSDVFIHTSLINMYAQSGEINYAQLVFDQSNFRDAISFTALIAGYVLWGYMDRARKLFD 216

[Close]

BLAST of Carg12146 vs. ExPASy TrEMBL
Match: A0A0A0LU28 (DYW_deaminase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G306800 PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.9e-25
Identity = 92/244 (37.70%), Postives = 101/244 (41.39%), Query Frame = 0


Query: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA------ 60
           MIR LSMSLS  + LVFFVRMI+SGV+P+SYTFPFL KSCAKLASAH GKQI A      
Sbjct: 100 MIRGLSMSLSPALALVFFVRMIYSGVEPNSYTFPFLLKSCAKLASAHEGKQIHAHVLKLG 159

Query: 61  ----------------------------------DAISFTAPIAGYAMWGNTDRARKVFG 118
                                             DAISFTA IAGYA+WG  DRAR++F 
Sbjct: 160 FVSDVFIHTSLINMYAQSGEMNNAQLVFDQSNFRDAISFTALIAGYALWGYMDRARQLFD 219

[Close]

BLAST of Carg12146 vs. ExPASy TrEMBL
Match: A0A6J1JHE6 (pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111485125 PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 4.3e-25
Identity = 90/244 (36.89%), Postives = 100/244 (40.98%), Query Frame = 0


Query: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA------ 60
           MIR LS+SLS V+ LVFF RMIH+GV+P+SYTFPFL KSCA+LASA  GKQI A      
Sbjct: 97  MIRGLSISLSPVLALVFFARMIHAGVEPNSYTFPFLLKSCARLASAREGKQIHAHVLKLG 156

Query: 61  ----------------------------------DAISFTAPIAGYAMWGNTDRARKVFG 118
                                             DAISFTA IAGY +WG  DRARK+F 
Sbjct: 157 FVSDVFIHTSLINMYAQSGEINYAQLVFDQSNFRDAISFTALIAGYVLWGYMDRARKLFD 216

[Close]

BLAST of Carg12146 vs. TAIR 10
Match: AT1G08070.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 4.0e-15
Identity = 54/158 (34.18%), Postives = 74/158 (46.84%), Query Frame = 0


Query: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA------ 60
           M R  ++S   V  L  +V MI  G+ P+SYTFPF+ KSCAK  +   G+QI        
Sbjct: 105 MFRGHALSSDPVSALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQIHGHVLKLG 164

Query: 61  ---DAISFTAPIAGYAMWGNTDRARKVFGEMPVRDV-----------------TARELFD 120
              D    T+ I+ Y   G  + A KVF + P RDV                  A++LFD
Sbjct: 165 CDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGYIENAQKLFD 224

Query: 121 DMPERDVVSWTVMIRVYTHMSSYTLEFEPVADEQTTSL 133
           ++P +DVVSW  MI  Y    +Y    E   D   T++
Sbjct: 225 EIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNV 262

[Close]

BLAST of Carg12146 vs. TAIR 10
Match: AT3G22690.1 (CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1685 (InterPro:IPR012881), Pentatricopeptide repeat (InterPro:IPR002885); BEST Arabidopsis thaliana protein match is: Tetratricopeptide repeat (TPR)-like superfamily protein (TAIR:AT2G29760.1); Has 49784 Blast hits to 14716 proteins in 280 species: Archae - 2; Bacteria - 10; Metazoa - 107; Fungi - 167; Plants - 48594; Viruses - 0; Other Eukaryotes - 904 (source: NCBI BLink). )

HSP 1 Score: 67.0 bits (162), Expect = 2.0e-11
Identity = 46/129 (35.66%), Postives = 60/129 (46.51%), Query Frame = 0


Query: 15  LVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA---------DAISFTAPIAG 74
           ++ F+RM++SG+ PD YTFPF   +CAK  +   G QI           D     + +  
Sbjct: 119 ILLFLRMMNSGISPDKYTFPFGLSACAKSRAKGNGIQIHGLIVKMGYAKDLFVQNSLVHF 178

Query: 75  YAMWGNTDRARKVFGEMPVRDVTARELFDDMPERDVVSWTVMIRVYTH----MSSYTLEF 131
           YA  G  D ARKV              FD+M ER+VVSWT MI  Y        +  L F
Sbjct: 179 YAECGELDSARKV--------------FDEMSERNVVSWTSMICGYARRDFAKDAVDLFF 233

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BLAST of Carg12146 vs. TAIR 10
Match: AT3G22690.2 (INVOLVED IN: photosystem II assembly, regulation of chlorophyll biosynthetic process, photosystem I assembly, thylakoid membrane organization, RNA modification; LOCATED IN: chloroplast; EXPRESSED IN: 13 plant structures; EXPRESSED DURING: LP.04 four leaves visible, 4 anthesis, petal differentiation and expansion stage, E expanded cotyledon stage, D bilateral stage; CONTAINS InterPro DOMAIN/s: Pentatricopeptide repeat (InterPro:IPR002885); BEST Arabidopsis thaliana protein match is: Tetratricopeptide repeat (TPR)-like superfamily protein (TAIR:AT2G29760.1). )

HSP 1 Score: 67.0 bits (162), Expect = 2.0e-11
Identity = 46/129 (35.66%), Postives = 60/129 (46.51%), Query Frame = 0


Query: 15  LVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA---------DAISFTAPIAG 74
           ++ F+RM++SG+ PD YTFPF   +CAK  +   G QI           D     + +  
Sbjct: 119 ILLFLRMMNSGISPDKYTFPFGLSACAKSRAKGNGIQIHGLIVKMGYAKDLFVQNSLVHF 178

Query: 75  YAMWGNTDRARKVFGEMPVRDVTARELFDDMPERDVVSWTVMIRVYTH----MSSYTLEF 131
           YA  G  D ARKV              FD+M ER+VVSWT MI  Y        +  L F
Sbjct: 179 YAECGELDSARKV--------------FDEMSERNVVSWTSMICGYARRDFAKDAVDLFF 233

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BLAST of Carg12146 vs. TAIR 10
Match: AT4G18840.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 7.7e-11
Identity = 52/189 (27.51%), Postives = 74/189 (39.15%), Query Frame = 0


Query: 1   MIRRLSMSLSAVMPLVFFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGGKQIRA------ 60
           +IR  + S +  + L  F  M+   V PD Y+F F+ K+CA       G+QI        
Sbjct: 111 VIRAYANSSTPEVALTVFREMLLGPVFPDKYSFTFVLKACAAFCGFEEGRQIHGLFIKSG 170

Query: 61  ----------------------------------DAISFTAPIAGYAMWGNTDRARKVFG 120
                                             DA+S+ + ++ Y   G  D AR +F 
Sbjct: 171 LVTDVFVENTLVNVYGRSGYFEIARKVLDRMPVRDAVSWNSLLSAYLEKGLVDEARALFD 230

Query: 121 EMPVRDV-----------------TARELFDDMPERDVVSWTVMIRVYTHMSSY--TLE- 130
           EM  R+V                  A+E+FD MP RDVVSW  M+  Y H+  Y   LE 
Sbjct: 231 EMEERNVESWNFMISGYAAAGLVKEAKEVFDSMPVRDVVSWNAMVTAYAHVGCYNEVLEV 290

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BLAST of Carg12146 vs. TAIR 10
Match: AT5G61800.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.0e-10
Identity = 46/129 (35.66%), Postives = 59/129 (45.74%), Query Frame = 0


Query: 17  FFVRMIHSGVKPDSYTFPFLFKSCAKLASAHGG----KQIRADAISF---------TAPI 76
           FFV M    V PD +TFPF+FK+CA  A  +G     K +   A+ F            I
Sbjct: 102 FFVEMRRRSVPPDFHTFPFVFKACA--AKKNGDLTLVKTLHCQALRFGLLSDLFTLNTLI 161

Query: 77  AGYAMWGNTDRARKVFGEMPVRDVT-----------------ARELFDDMPERDVVSWTV 116
             Y++    D A ++F E P RDV                  ARELFD MP RD+VSW  
Sbjct: 162 RVYSLIAPIDSALQLFDENPQRDVVTYNVLIDGLVKAREIVRARELFDSMPLRDLVSWNS 221

The following BLAST results are available for this feature:

BLAST of Carg12146 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021800.1	4.3e-112	100.00	Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita a...	[more]
KAG6587914.1	4.2e-43	86.79	hypothetical protein SDJN03_16479, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6587915.1	1.7e-39	80.00	Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita a...	[more]
XP_038878535.1	5.5e-27	38.93	pentatricopeptide repeat-containing protein At1g08070, chloroplastic isoform X1 ...	[more]
KAG6589865.1	3.6e-26	36.33	Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita a...	[more]

BLAST of Carg12146 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LN01	5.6e-14	34.18	Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidop...	[more]
Q9LUJ2	2.9e-10	35.66	Pentatricopeptide repeat-containing protein At3g22690 OS=Arabidopsis thaliana OX...	[more]
O49399	1.1e-09	27.51	Pentatricopeptide repeat-containing protein At4g18840 OS=Arabidopsis thaliana OX...	[more]
Q9FLS9	1.4e-09	35.66	Pentatricopeptide repeat-containing protein At5g61800 OS=Arabidopsis thaliana OX...	[more]
Q9SIL5	3.2e-09	36.21	Pentatricopeptide repeat-containing protein At2g20540 OS=Arabidopsis thaliana OX...	[more]

BLAST of Carg12146 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TTJ1	3.9e-26	38.11	Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3CMX0	3.9e-26	38.11	pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Cucumis ...	[more]
A0A6J1HAU9	6.6e-26	37.70	pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Cucurbit...	[more]
A0A0A0LU28	1.9e-25	37.70	DYW_deaminase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G3068...	[more]
A0A6J1JHE6	4.3e-25	36.89	pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Cucurbit...	[more]

BLAST of Carg12146 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08070.1	4.0e-15	34.18	Tetratricopeptide repeat (TPR)-like superfamily protein	[more]
AT3G22690.1	2.0e-11	35.66	CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1685 (InterPro:IPR012...	[more]
AT3G22690.2	2.0e-11	35.66	INVOLVED IN: photosystem II assembly, regulation of chlorophyll biosynthetic pro...	[more]
AT4G18840.1	7.7e-11	27.51	Pentatricopeptide repeat (PPR-like) superfamily protein	[more]
AT5G61800.1	1.0e-10	35.66	Pentatricopeptide repeat (PPR) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 12..118 e-value: 1.9E-12 score: 49.2
IPR002885	Pentatricopeptide repeat	TIGRFAM	TIGR00756	TIGR00756	coord: 58..86 e-value: 1.9E-4 score: 19.4
IPR002885	Pentatricopeptide repeat	PFAM	PF01535	PPR	coord: 63..85 e-value: 0.011 score: 15.9
IPR002885	Pentatricopeptide repeat	PROSITE	PS51375	PPR	coord: 55..89 score: 8.582755
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 174..201
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 170..209
None	No IPR available	PANTHER	PTHR47924:SF46	PENTATRICOPEPTIDE REPEAT PROTEIN-RELATED	coord: 1..115
None	No IPR available	PANTHER	PTHR47924	PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN	coord: 1..115

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12146-RA	Carg12146-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding