Carg11823 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11823
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type domain-containing protein
Location	Carg_Chr05: 958231 .. 958923 (-)
RNA-Seq Expression	Carg11823
Synteny	Carg11823

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAGCGGGTTTGGGGAATCGACAAGTAGGCAACCCCAAGGCCCTTCTTATTCCGATAACAATGGTGATGCTAGTAATTTTGAATGCAATATTTGCCTGGATTTAGCTCAGGATCCTATTGTAACCCTATGCGGTCATCTTTTCTGTTGGCCATGCCTTTACAAGTGGCTGCACATTCATTCCCATCCCCAGGAATGTCCAGTTTGCAAGGCTCTCATAGAAGAACAAAAATTGGTTCCCTTATATGGTAGGGGAAAGACATCGACCGACCCGAGATCGAAAAGTATTCCTGGAATCAATATTCCTAATCGTCCTGCTGGACAGAGACCTGAAGCAGCAGGCCCTCCCCCAGAACCAAATCCTTTTCCCCACAATGGGTTTGGATTCATGGGAGGTCTTGGCGGGTTTGCCCCAATGGCATCGACGAGGTTTGGCAATTTCACTTTGTCTGCGGCTTTTGGTGGTCTTGTCCCATCTTTATTCAACTTTCAGATGCATGGATATGCAGATCCAAGCATGTATGGTGCAGGTGCTAACGTTCCATATGGTTTTCCTAACATGTTTCATGGTAGCCATGGTCACGCCCATGGCTACTATCACCATCTCCGTCAAGGTCAGCAAGATTATTACCTGAAGCGGCTGCTTTTGTTTATTGGTTTCTGTGTTCTTCTTGCCCTAATCTGGCAATAG

mRNA sequence

ATGGCGAGCGGGTTTGGGGAATCGACAAGTAGGCAACCCCAAGGCCCTTCTTATTCCGATAACAATGGTGATGCTAGTAATTTTGAATGCAATATTTGCCTGGATTTAGCTCAGGATCCTATTGTAACCCTATGCGGTCATCTTTTCTGTTGGCCATGCCTTTACAAGTGGCTGCACATTCATTCCCATCCCCAGGAATGTCCAGTTTGCAAGGCTCTCATAGAAGAACAAAAATTGGTTCCCTTATATGGTAGGGGAAAGACATCGACCGACCCGAGATCGAAAAGTATTCCTGGAATCAATATTCCTAATCGTCCTGCTGGACAGAGACCTGAAGCAGCAGGCCCTCCCCCAGAACCAAATCCTTTTCCCCACAATGGGTTTGGATTCATGGGAGGTCTTGGCGGGTTTGCCCCAATGGCATCGACGAGGTTTGGCAATTTCACTTTGTCTGCGGCTTTTGGTGGTCTTGTCCCATCTTTATTCAACTTTCAGATGCATGGATATGCAGATCCAAGCATGTATGGTGCAGGTGCTAACGTTCCATATGGTTTTCCTAACATGTTTCATGGTAGCCATGGTCACGCCCATGGCTACTATCACCATCTCCGTCAAGGTCAGCAAGATTATTACCTGAAGCGGCTGCTTTTGTTTATTGGTTTCTGTGTTCTTCTTGCCCTAATCTGGCAATAG

Coding sequence (CDS)

ATGGCGAGCGGGTTTGGGGAATCGACAAGTAGGCAACCCCAAGGCCCTTCTTATTCCGATAACAATGGTGATGCTAGTAATTTTGAATGCAATATTTGCCTGGATTTAGCTCAGGATCCTATTGTAACCCTATGCGGTCATCTTTTCTGTTGGCCATGCCTTTACAAGTGGCTGCACATTCATTCCCATCCCCAGGAATGTCCAGTTTGCAAGGCTCTCATAGAAGAACAAAAATTGGTTCCCTTATATGGTAGGGGAAAGACATCGACCGACCCGAGATCGAAAAGTATTCCTGGAATCAATATTCCTAATCGTCCTGCTGGACAGAGACCTGAAGCAGCAGGCCCTCCCCCAGAACCAAATCCTTTTCCCCACAATGGGTTTGGATTCATGGGAGGTCTTGGCGGGTTTGCCCCAATGGCATCGACGAGGTTTGGCAATTTCACTTTGTCTGCGGCTTTTGGTGGTCTTGTCCCATCTTTATTCAACTTTCAGATGCATGGATATGCAGATCCAAGCATGTATGGTGCAGGTGCTAACGTTCCATATGGTTTTCCTAACATGTTTCATGGTAGCCATGGTCACGCCCATGGCTACTATCACCATCTCCGTCAAGGTCAGCAAGATTATTACCTGAAGCGGCTGCTTTTGTTTATTGGTTTCTGTGTTCTTCTTGCCCTAATCTGGCAATAG

Protein sequence

MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGANVPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ

Homology

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BLAST of Carg11823 vs. NCBI nr
Match: KAG6598412.1 (E3 ubiquitin ligase rnf-5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 489.2 bits (1258), Expect = 2.0e-134
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 0


Query: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 17  MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 76

Query: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 77  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 136

Query: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 137 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 196

Query: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 231
           VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ
Sbjct: 197 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 246

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BLAST of Carg11823 vs. NCBI nr
Match: XP_023547002.1 (E3 ubiquitin-protein ligase RNF185-like [Cucurbita pepo subsp. pepo] >XP_023547003.1 E3 ubiquitin-protein ligase RNF185-like [Cucurbita pepo subsp. pepo] >KAG7029364.1 RING finger protein 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 489.2 bits (1258), Expect = 2.0e-134
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 0


Query: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 231
           VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ
Sbjct: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

[Close]

BLAST of Carg11823 vs. NCBI nr
Match: XP_022962412.1 (E3 ubiquitin-protein ligase RNF185-like [Cucurbita moschata] >XP_022962413.1 E3 ubiquitin-protein ligase RNF185-like [Cucurbita moschata])

HSP 1 Score: 488.0 bits (1255), Expect = 4.4e-134
Identity = 229/230 (99.57%), Postives = 230/230 (100.00%), Query Frame = 0


Query: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRP+GQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPSGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 231
           VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ
Sbjct: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

[Close]

BLAST of Carg11823 vs. NCBI nr
Match: XP_022996954.1 (E3 ubiquitin-protein ligase RNF185-like [Cucurbita maxima] >XP_022996955.1 E3 ubiquitin-protein ligase RNF185-like [Cucurbita maxima])

HSP 1 Score: 486.1 bits (1250), Expect = 1.7e-133
Identity = 228/230 (99.13%), Postives = 229/230 (99.57%), Query Frame = 0


Query: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           M+ GFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MSRGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 231
           VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ
Sbjct: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

[Close]

BLAST of Carg11823 vs. NCBI nr
Match: XP_038884023.1 (E3 ubiquitin ligase rnf-5 [Benincasa hispida])

HSP 1 Score: 477.6 bits (1228), Expect = 5.9e-131
Identity = 225/230 (97.83%), Postives = 226/230 (98.26%), Query Frame = 0


Query: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGESTSRQPQ PS SDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 231
            PYGFPNMFHGSHGHAHGYYH LRQGQQDYYLKRLLLFIGFCVLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHAHGYYHQLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

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BLAST of Carg11823 vs. ExPASy Swiss-Prot
Match: Q09463 (E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 3.2e-31
Identity = 89/242 (36.78%), Postives = 120/242 (49.59%), Query Frame = 0


Query: 9   TSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECP 68
           T    + P+ S N  +++ FECNICLD A+D +V+LCGHLFCWPCL +WL    + Q CP
Sbjct: 5   TKAPSEEPTSSSNKDESARFECNICLDAAKDAVVSLCGHLFCWPCLSQWLDTRPNNQVCP 64

Query: 69  VCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNPFPHNGF 128
           VCK+ I+  K+VP+YGRG  S+DPR K      +P RP GQR E     P P  F    +
Sbjct: 65  VCKSAIDGNKVVPIYGRGGDSSDPRKK------VPPRPKGQRSE-----PPPQSFAGFNW 124

Query: 129 GFMGG-LGGFAPMASTRFGNFTLSAAF---------GGLVPSLFNFQMHGYADPSMYGAG 188
           G  GG +GG  P     FG  T++  F          G+ P  F     G  +     AG
Sbjct: 125 GGDGGMMGGGGPNVHFSFGIGTVNGLFPLMFMLPFIQGIFPLSFVASFFGNGNQGAAAAG 184

Query: 189 ANVPYGFPNMFHGSHGHAHGYYH-----------HLRQGQQDYYLKRLLLFIGFCVLLAL 230
                G      G+H H HG+ H             R  Q++ YL  +  +IGF +L  L
Sbjct: 185 GGNGGGNDGN-DGTHAHTHGHTHGPRGHGESAAPGSRMAQEEEYLSNIFKYIGFFMLFWL 234

[Close]

BLAST of Carg11823 vs. ExPASy Swiss-Prot
Match: Q96GF1 (E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.4e-26
Identity = 73/143 (51.05%), Postives = 84/143 (58.74%), Query Frame = 0


Query: 6   GESTSRQPQ-----GPSYSDN-----NGDASNFECNICLDLAQDPIVTLCGHLFCWPCLY 65
           G S S  P+     GPS S N      G  S FECNICLD A+D +++LCGHLFCWPCL+
Sbjct: 5   GPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLH 64

Query: 66  KWLHIHSHPQECPVCKALIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAA 125
           +WL    + Q CPVCKA I   K++PLYGRG T   DPR K+      P RP GQRPE  
Sbjct: 65  QWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-- 124

Query: 126 GPPPEPNPFPHNGFGFMGGLGGF 138
              PE N     GFGF  G GGF
Sbjct: 125 ---PE-NRGGFQGFGF--GDGGF 133

[Close]

BLAST of Carg11823 vs. ExPASy Swiss-Prot
Match: Q5RFK9 (E3 ubiquitin-protein ligase RNF185 OS=Pongo abelii OX=9601 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.4e-26
Identity = 73/143 (51.05%), Postives = 84/143 (58.74%), Query Frame = 0


Query: 6   GESTSRQPQ-----GPSYSDN-----NGDASNFECNICLDLAQDPIVTLCGHLFCWPCLY 65
           G S S  P+     GPS S N      G  S FECNICLD A+D +++LCGHLFCWPCL+
Sbjct: 5   GPSASSSPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLH 64

Query: 66  KWLHIHSHPQECPVCKALIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAA 125
           +WL    + Q CPVCKA I   K++PLYGRG T   DPR K+      P RP GQRPE  
Sbjct: 65  QWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-- 124

Query: 126 GPPPEPNPFPHNGFGFMGGLGGF 138
              PE N     GFGF  G GGF
Sbjct: 125 ---PE-NRGGFQGFGF--GDGGF 133

[Close]

BLAST of Carg11823 vs. ExPASy Swiss-Prot
Match: Q91YT2 (E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.1e-26
Identity = 69/129 (53.49%), Postives = 79/129 (61.24%), Query Frame = 0


Query: 15  GPSYSDN-----NGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPV 74
           GPS S N      G  S FECNICLD A+D +++LCGHLFCWPCL++WL    + Q CPV
Sbjct: 19  GPSGSSNGTGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPV 78

Query: 75  CKALIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNPFPHNGF 134
           CKA I   K++PLYGRG T   DPR K+      P RP GQRPE     PE N     GF
Sbjct: 79  CKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-----PE-NRGGFQGF 133

Query: 135 GFMGGLGGF 138
           GF  G GGF
Sbjct: 139 GF--GDGGF 133

[Close]

BLAST of Carg11823 vs. ExPASy Swiss-Prot
Match: Q568Y3 (E3 ubiquitin-protein ligase RNF185 OS=Rattus norvegicus OX=10116 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.1e-26
Identity = 69/129 (53.49%), Postives = 79/129 (61.24%), Query Frame = 0


Query: 15  GPSYSDN-----NGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPV 74
           GPS S N      G  S FECNICLD A+D +++LCGHLFCWPCL++WL    + Q CPV
Sbjct: 19  GPSGSSNGTGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPV 78

Query: 75  CKALIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNPFPHNGF 134
           CKA I   K++PLYGRG T   DPR K+      P RP GQRPE     PE N     GF
Sbjct: 79  CKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-----PE-NRGGFQGF 133

Query: 135 GFMGGLGGF 138
           GF  G GGF
Sbjct: 139 GF--GDGGF 133

[Close]

BLAST of Carg11823 vs. ExPASy TrEMBL
Match: A0A6J1HCN0 (E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita moschata OX=3662 GN=LOC111462860 PE=4 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.1e-134
Identity = 229/230 (99.57%), Postives = 230/230 (100.00%), Query Frame = 0


Query: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRP+GQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPSGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 231
           VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ
Sbjct: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

[Close]

BLAST of Carg11823 vs. ExPASy TrEMBL
Match: A0A6J1KA44 (E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita maxima OX=3661 GN=LOC111492032 PE=4 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 8.0e-134
Identity = 228/230 (99.13%), Postives = 229/230 (99.57%), Query Frame = 0


Query: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           M+ GFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MSRGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 231
           VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ
Sbjct: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

[Close]

BLAST of Carg11823 vs. ExPASy TrEMBL
Match: A0A6J1GGW7 (RING finger protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111454080 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 6.0e-129
Identity = 223/234 (95.30%), Postives = 225/234 (96.15%), Query Frame = 0


Query: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGESTSRQPQ PS SDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPG+NIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAMIEEQKLVPLYGRGKTSTDPRSKSIPGVNIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180
           NPF HNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFAHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 VPYGFPNMFHGSHG----HAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 231
            PYGFPNMFHGSHG    HAHGYYHHLRQGQQDYYLKRLL FIGFCVLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHGHAHAHGYYHHLRQGQQDYYLKRLLFFIGFCVLLALIWQ 234

[Close]

BLAST of Carg11823 vs. ExPASy TrEMBL
Match: A0A6J1CT42 (E3 ubiquitin-protein ligase RNF185 OS=Momordica charantia OX=3673 GN=LOC111014071 PE=4 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 7.8e-129
Identity = 221/230 (96.09%), Postives = 223/230 (96.96%), Query Frame = 0


Query: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGESTSRQPQ PS SDNN DASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180
           N FPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGA 
Sbjct: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180

Query: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 231
            PYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLL+ IGFCVLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 230

[Close]

BLAST of Carg11823 vs. ExPASy TrEMBL
Match: A0A0A0LLD2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G360850 PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 3.0e-128
Identity = 221/230 (96.09%), Postives = 222/230 (96.52%), Query Frame = 0


Query: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           M SGFGESTSRQPQ PS SDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MESGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 231
            PYGFPNMFHGSHGHAHGYYH LRQGQQDYYLKRLL  IGF VLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHAHGYYHQLRQGQQDYYLKRLLFLIGFFVLLALIWQ 230

[Close]

BLAST of Carg11823 vs. TAIR 10
Match: AT1G19310.1 (RING/U-box superfamily protein )

HSP 1 Score: 289.3 bits (739), Expect = 2.8e-78
Identity = 142/211 (67.30%), Postives = 166/211 (78.67%), Query Frame = 0


Query: 21  NNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCKALIEEQKLV 80
           NN D+SNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLH+HS  ++CPVCKA+IEE +LV
Sbjct: 14  NNNDSSNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQSKDCPVCKAVIEEDRLV 73

Query: 81  PLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNPFPHNGFGFMGGLGGF-AP 140
           PLYGRGK+S DPRSKSIPG+ +PNRP+GQRPE A PP   + F H+  GF G +GGF AP
Sbjct: 74  PLYGRGKSSADPRSKSIPGLEVPNRPSGQRPETAQPPDPNHGFAHH-HGFGGFMGGFAAP 133

Query: 141 MASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN--VPYGFPNMFHGSHGHAH 200
           MAS RFGN TLSAAFGGL+PSLFN   HG+ D +MYGA A+   P+GF N FHG H H H
Sbjct: 134 MASARFGNVTLSAAFGGLIPSLFNLHFHGFPDAAMYGAAASGGFPHGFSNPFHGGHSHMH 193

Query: 201 GYYHHL-RQGQQDYYLKRLLLFIGFCVLLAL 228
            Y  H  RQGQQD++L+ LLL +   V+ +L
Sbjct: 194 SYQRHTGRQGQQDHHLRILLLIVFVVVVFSL 223

[Close]

BLAST of Carg11823 vs. TAIR 10
Match: AT2G23780.1 (RING/U-box superfamily protein )

HSP 1 Score: 253.4 bits (646), Expect = 1.7e-67
Identity = 130/230 (56.52%), Postives = 159/230 (69.13%), Query Frame = 0


Query: 6   GESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQ 65
           GES++      S +D N    +FECNIC +LAQDPIVTLCGHLFCWPCLY+WLH HSH Q
Sbjct: 4   GESSTSTSYSDSNNDTNDQGGDFECNICFELAQDPIVTLCGHLFCWPCLYRWLHHHSHSQ 63

Query: 66  ECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP---NP 125
           ECPVCKA++++ KLVPLYGRGK  TDPRSK  PG+ IPNRP GQRPE A PPP+P   + 
Sbjct: 64  ECPVCKAVVQDDKLVPLYGRGKNQTDPRSKRYPGLRIPNRPTGQRPETAAPPPQPEAASN 123

Query: 126 FPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGANVP 185
           F + G G MGG+     MA+TRFGNF  S  FGGL+PSLFNFQ HG+ D ++YG+    P
Sbjct: 124 FFNYGIGLMGGI--MPMMATTRFGNF--SMGFGGLLPSLFNFQFHGFHDATLYGSTPGYP 183

Query: 186 Y-GFPNMFHGSHGHAHGYYHHLRQG--QQDYYLKRLLLFIGFCVLLALIW 230
           Y G+ N F G      G    + +G  Q D +LK +L F+G CV++ LIW
Sbjct: 184 YGGYHNGFRGV--PPRGQERPMARGGNQSDAFLKNILFFVGICVVIFLIW 227

[Close]

BLAST of Carg11823 vs. TAIR 10
Match: AT1G74990.1 (RING/U-box superfamily protein )

HSP 1 Score: 158.3 bits (399), Expect = 7.4e-39
Identity = 77/133 (57.89%), Postives = 91/133 (68.42%), Query Frame = 0


Query: 19  SDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCKALIEEQK 78
           ++ +  ++NF CNICL+LA++PIVTLCGHLFCWPCLYKWLH HS    CPVCKAL++E  
Sbjct: 8   NEEDDASNNFGCNICLELAREPIVTLCGHLFCWPCLYKWLHYHSKSNHCPVCKALVKEDT 67

Query: 79  LVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNPFPHNGFGFMGGLGGFA 138
           LVPLYG GK S+DPRSK   G+ +PNRPA  R E A P  E     H+G  F GG   FA
Sbjct: 68  LVPLYGMGKPSSDPRSKLNSGVTVPNRPAATRTETARPRLEQR---HHGSSFFGGHSSFA 127

Query: 139 PM-ASTRFGNFTL 151
            M    RF NF L
Sbjct: 128 AMPTGLRFSNFLL 137

[Close]

BLAST of Carg11823 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 110.2 bits (274), Expect = 2.3e-24
Identity = 50/110 (45.45%), Postives = 69/110 (62.73%), Query Frame = 0


Query: 7   ESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHS---- 66
           +S   +P   S   ++ D SNF+CNICLD  Q+P+VTLCGHLFCWPC++KWL + S    
Sbjct: 25  KSKKPEPITVSVPSDDTDDSNFDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTS 84

Query: 67  ----HPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAG 109
                 ++CPVCK+ +    LVPLYGRG+ +T    K+    ++P RP G
Sbjct: 85  DEYQRHRQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKN----SVPKRPVG 130

[Close]

BLAST of Carg11823 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 110.2 bits (274), Expect = 2.3e-24
Identity = 50/110 (45.45%), Postives = 69/110 (62.73%), Query Frame = 0


Query: 7   ESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHS---- 66
           +S   +P   S   ++ D SNF+CNICLD  Q+P+VTLCGHLFCWPC++KWL + S    
Sbjct: 25  KSKKPEPITVSVPSDDTDDSNFDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTS 84

Query: 67  ----HPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAG 109
                 ++CPVCK+ +    LVPLYGRG+ +T    K+    ++P RP G
Sbjct: 85  DEYQRHRQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKN----SVPKRPVG 130

The following BLAST results are available for this feature:

BLAST of Carg11823 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6598412.1	2.0e-134	100.00	E3 ubiquitin ligase rnf-5, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023547002.1	2.0e-134	100.00	E3 ubiquitin-protein ligase RNF185-like [Cucurbita pepo subsp. pepo] >XP_0235470...	[more]
XP_022962412.1	4.4e-134	99.57	E3 ubiquitin-protein ligase RNF185-like [Cucurbita moschata] >XP_022962413.1 E3 ...	[more]
XP_022996954.1	1.7e-133	99.13	E3 ubiquitin-protein ligase RNF185-like [Cucurbita maxima] >XP_022996955.1 E3 ub...	[more]
XP_038884023.1	5.9e-131	97.83	E3 ubiquitin ligase rnf-5 [Benincasa hispida]	[more]

BLAST of Carg11823 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q09463	3.2e-31	36.78	E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1	[more]
Q96GF1	1.4e-26	51.05	E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1	[more]
Q5RFK9	1.4e-26	51.05	E3 ubiquitin-protein ligase RNF185 OS=Pongo abelii OX=9601 GN=RNF185 PE=2 SV=1	[more]
Q91YT2	3.1e-26	53.49	E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1	[more]
Q568Y3	3.1e-26	53.49	E3 ubiquitin-protein ligase RNF185 OS=Rattus norvegicus OX=10116 GN=Rnf185 PE=2 ...	[more]

BLAST of Carg11823 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HCN0	2.1e-134	99.57	E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KA44	8.0e-134	99.13	E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita maxima OX=3661 GN=LOC111492...	[more]
A0A6J1GGW7	6.0e-129	95.30	RING finger protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111454080 PE=4 SV=1	[more]
A0A6J1CT42	7.8e-129	96.09	E3 ubiquitin-protein ligase RNF185 OS=Momordica charantia OX=3673 GN=LOC11101407...	[more]
A0A0A0LLD2	3.0e-128	96.09	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G360850 P...	[more]

BLAST of Carg11823 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G19310.1	2.8e-78	67.30	RING/U-box superfamily protein	[more]
AT2G23780.1	1.7e-67	56.52	RING/U-box superfamily protein	[more]
AT1G74990.1	7.4e-39	57.89	RING/U-box superfamily protein	[more]
AT4G03510.1	2.3e-24	45.45	RING membrane-anchor 1	[more]
AT4G03510.2	2.3e-24	45.45	RING membrane-anchor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 30..70 e-value: 6.4E-8 score: 42.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 30..71 score: 13.256433
None	No IPR available	PFAM	PF13923	zf-C3HC4_2	coord: 29..70 e-value: 1.9E-8 score: 34.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 84..122
None	No IPR available	PANTHER	PTHR12313:SF39	OS12G0636000 PROTEIN	coord: 2..230
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 28..72 e-value: 4.45876E-31 score: 106.62
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 26..83
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 17..84 e-value: 6.8E-18 score: 65.8
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 2..230
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 45..54

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11823-RA	Carg11823-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071712	ER-associated misfolded protein catabolic process
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0044390	ubiquitin-like protein conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity