Carg11454 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg11454
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionmembrane protein of ER body 2-like
LocationCarg_Chr17: 9101090 .. 9104021 (-)
RNA-Seq ExpressionCarg11454
SyntenyCarg11454
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGATAAATTTATTTTTAATTATATACACTTCCTTATATAAGGTTGTTTATAACGTACAAAAACACACTTTCAATCTTTAAGTTTGAAGTTAGTTTCAATTTAGTCTATAAGTTCAAAATAGACAGTTTAATTGAGATTTAGTAGAAAATAGTTTTAAAAAGTTGCTCAATCTACTTTGATCTTTAATTGGCTAAATGTTAATGGATTGTTTGTATTTTGTTGACTTGGTAATAGTATTGATGGAATAATTTTTTTTTTCTTTTTTGGGACTGATCTTTCTCCCTCCTCTCCCCTCCGCCAGCTTCTGAATCATTCAACTTCTTCAAAATGTTAAATGATTCAATAAAATCGTATAACATATACTGTTCCAAGTGCCAGAACTGTATCAACAATTTGATTCAAAGTGTTTTGAAATTAGAAGATGTTGAAGAATCATCTGCAAAATTTTCATACGAAGATGAAAAAGACATTCCAAAAAAGTGTTATGAAGTTCTTTATAAGTTTCTCAATTTGCATACCATACATTGTCCTGCTTGCACGAATTATGTCAGAGAGGTGACAATCCAACATGCTCCAAAACAATCCACGACTCTTGTACAAGTTCCAAAACCATCCATTCATGGTACAGGTGAGTTTGATGTAAAAAAGTGCCAAAATTTCATGCTAAATTTATGTGTTTTAGTTTTAGAATTGTTTGATAATGCATTGAGATTAATACCATTGTGTCGTATAAACTCTTTTTCCACTCTCGTCTTTTCTATACTCATTATAAGCAGATGAAAAAATTATAGACCATCAAGAAGGCTTAGTTATCACGATCTTCCATCCTGATGAACCAAGCACTAGAGTTAGAAGGTGGGAAATTCGTAGACTTATAGTACTTGGTGGTTTAATGGAATCAATTACAAGTTTAGCTATTGTAACTGCAGCAATGAGTGCAAAGATTTCTGATGGTAATGGTTTTCATTTCCTCTATTTCCCTTTTTCTTAAATAATGATTAGGCGATCTCTAGTTGATCTTGGATTGGGATTCTTGCAGGAAATATAGTAGCTTTGGCGTGGACAAATTTGATAGCTGGACTCTTTATTATTAGGCATGATGTAAGCATTTACCTTTTGTCTTTTGGGTTTTCAAGCTTTTGATGTAGTGAAACGTATGAAGTAAATGTACAGGTATCAAGATTGCAGAAGAAGAGAAAAATACCTGGAATTGAAGTGGACGGCAATAACTACAAAGAAGTATTGAAGGAAAAAATGTATAGTCTTCTCTGTTTTATCATCGCCTACTTATCTTTCTTGTGTTTTGGGTTAGTGCCACCACTGGTTTATGCACTCTCATCCCTCAAGATCAGAAACAAAAACCTCAAAATCATAGCGGCTGCAGGAGCTTCCCTTTCATGCACTGCACTTCTTGCTGTCCAGAAAGCTCACATTAATCAAAAGCCAAGACATAGGCTAGTTTATGTCAAAACTGCAGCCGTAGATGTTTCAGTGGGAGTTGGGGCTTTTGGCATATCATATCTAGCAGGTTACATGTTTGGACACCTTGCATAGGAACTTCTGTGGGACTTGTGAAACTCAGCACACCCACTTCATCATTTTGCTTTGGTTTCTGCAGAATATGGTTATACATAAGTCTTTGATCAACTTCTATGCTGGTTCAGTTGGTAAAGAAATGGGATATCTTAACTGGAATTGCTATGTTATTTAACAACCCAGTTCAGCACTTTATTCAATGATGCAATTCTTTATCGCCAGCTTGTCGGTTTACTTCAATATCTCACATTTACCCGACTCGACATTGCCTTTGTAATCAATAGAGTAGGTCAATTCATGATCATCCCACAATTCTTCTTTTTTTCTTTTCTTTGTGGATCAAGCAACCTGGCATTATGTTTAGTTGAGAAGATTTGTCTCTCACAACCCTTTTGTTGTAACTCTGTCAGGTTGGTGATACATCTGATACATCTGAATTGTCGTTCTACATATGGTTTTATTGCTTTTCTTGGCTCCAGTCCTATATCCTGGTTTGCCAACAACCAACACACCATTTCCTGCTCATCCACACTCTGGCGAAATATCGCTTGTTGGCTACTGTAACTGCTGATCTCTTTTGGTTGTGGCAGTTCCATTGTGATCTTCTTGTTCCTTTGACAGCCTCCTCCCCCGCTATGGTGTGACAATGTCAATTTTACTTGCAAAAAAACCAGTGTTGCTGCCATTTATATCTTCCATTTTGATCGTGACAAGGTTGTTCACAAGAACATTTTTGTTCACTTTATATCTTCCAAAGATCAGTTTGTGGATTTGCTCACCAAGCTGTTCTCATCCAAATCTTCTTGTCTTCTCGTGACCAACTTCTGACTTCTACCCAGTCTTCAGTTTGAGGGGGCATGTTATGAAGTGTTCTACATATTATCCGTGGTCATATTTTATGCCTTAAATTTTAAATAATGTTATTGTAATCTGCCAAATAAATGCTTTTAACTTATTTGCTTGTTCTCTCGATGTACATAAAGGGGTCATCACCACTATGAAATTCATGAAGGAAATGACATTCAATTTTGTCTATCTTCCTCGGTGTTTCTATAGCTCTCACTTATGGTTTCATGCTTCTATGTACATGAATATTAAGATTCATACTTTCTATACTGCCTCTGATATGCTCACAAAGCATAAGTTACAGCTACTGTTGGGTTTGCCTTACTTGAAAAAGGTTAGTATTAAATAAATCAGAAGCGGCTGCCAAAAGCTACAGGGGAGAAGATTGATAGACAATAATTCTCGAGAGTTAGAGAGTAAGTATTCTATGTGAATAAAAGCTTCAGCTCCCCCCTCCCTTCTCAGTGGATGCAGGCCTTGCTTTCAGAATCACTTTAATTCTGTGTTGTGCATGAACTTCATCTCTTATCTCCCTAATTCCTTCTGATTG

mRNA sequence

TGATAAATTTATTTTTAATTATATACACTTCCTTATATAAGGTTGTTTATAACGTACAAAAACACACTTTCAATCTTTAAGTTTGAAGTTAGTTTCAATTTAGTCTATAAGTTCAAAATAGACAGTTTAATTGAGATTTAGTAGAAAATAGTTTTAAAAAGTTGCTCAATCTACTTTGATCTTTAATTGGCTAAATGTTAATGGATTGTTTGTATTTTGTTGACTTGGTAATAGTATTGATGGAATAATTTTTTTTTTCTTTTTTGGGACTGATCTTTCTCCCTCCTCTCCCCTCCGCCAGCTTCTGAATCATTCAACTTCTTCAAAATGTTAAATGATTCAATAAAATCGTATAACATATACTGTTCCAAGTGCCAGAACTGTATCAACAATTTGATTCAAAGTGTTTTGAAATTAGAAGATGTTGAAGAATCATCTGCAAAATTTTCATACGAAGATGAAAAAGACATTCCAAAAAAGTGTTATGAAGTTCTTTATAAGTTTCTCAATTTGCATACCATACATTGTCCTGCTTGCACGAATTATGTCAGAGAGGTGACAATCCAACATGCTCCAAAACAATCCACGACTCTTGTACAAGTTCCAAAACCATCCATTCATGGTACAGATGAAAAAATTATAGACCATCAAGAAGGCTTAGTTATCACGATCTTCCATCCTGATGAACCAAGCACTAGAGTTAGAAGGTGGGAAATTCGTAGACTTATAGTACTTGGTGGTTTAATGGAATCAATTACAAGTTTAGCTATTGTAACTGCAGCAATGAGTGCAAAGATTTCTGATGGAAATATAGTAGCTTTGGCGTGGACAAATTTGATAGCTGGACTCTTTATTATTAGGCATGATGTATCAAGATTGCAGAAGAAGAGAAAAATACCTGGAATTGAAGTGGACGGCAATAACTACAAAGAAGTATTGAAGGAAAAAATGTATAGTCTTCTCTGTTTTATCATCGCCTACTTATCTTTCTTGTGTTTTGGGTTAGTGCCACCACTGGTTTATGCACTCTCATCCCTCAAGATCAGAAACAAAAACCTCAAAATCATAGCGGCTGCAGGAGCTTCCCTTTCATGCACTGCACTTCTTGCTGTCCAGAAAGCTCACATTAATCAAAAGCCAAGACATAGGCTAGTTTATGTCAAAACTGCAGCCGTAGATGTTTCAGTGGGAGTTGGGGCTTTTGGCATATCATATCTAGCAGGTTACATGTTTGGACACCTTGCATAGGAACTTCTGTGGGACTTGTGAAACTCAGCACACCCACTTCATCATTTTGCTTTGGTTTCTGCAGAATATGGTTATACATAAGTCTTTGATCAACTTCTATGCTGGTTCAGTTGGTAAAGAAATGGGATATCTTAACTGGAATTGCTATGTTATTTAACAACCCAGTTCAGCACTTTATTCAATGATGCAATTCTTTATCGCCAGCTTGTCGGTTTACTTCAATATCTCACATTTACCCGACTCGACATTGCCTTTGTAATCAATAGAGTAGGTTGGTGATACATCTGATACATCTGAATTGTCGTTCTACATATGGTTTTATTGCTTTTCTTGGCTCCAGTCCTATATCCTGGTTTGCCAACAACCAACACACCATTTCCTGCTCATCCACACTCTGGCGAAATATCGCTTGTTGGCTACTGTAACTGCTGATCTCTTTTGGTTGTGGCAGTTCCATTGTGATCTTCTTGTTCCTTTGACAGCCTCCTCCCCCGCTATGGTGTGACAATGTCAATTTTACTTGCAAAAAAACCAGTGTTGCTGCCATTTATATCTTCCATTTTGATCGTGACAAGGTTGTTCACAAGAACATTTTTGTTCACTTTATATCTTCCAAAGATCAGTTTGTGGATTTGCTCACCAAGCTGTTCTCATCCAAATCTTCTTGTCTTCTCGTGACCAACTTCTGACTTCTACCCAGTCTTCAGTTTGAGGGGGCATGTTATGAAGTGTTCTACATATTATCCGTGGTCATATTTTATGCCTTAAATTTTAAATAATGTTATTGTAATCTGCCAAATAAATGCTTTTAACTTATTTGCTTGTTCTCTCGATGTACATAAAGGGGTCATCACCACTATGAAATTCATGAAGGAAATGACATTCAATTTTGTCTATCTTCCTCGGTGTTTCTATAGCTCTCACTTATGGTTTCATGCTTCTATGTACATGAATATTAAGATTCATACTTTCTATACTGCCTCTGATATGCTCACAAAGCATAAGTTACAGCTACTGTTGGGTTTGCCTTACTTGAAAAAGGTTAGTATTAAATAAATCAGAAGCGGCTGCCAAAAGCTACAGGGGAGAAGATTGATAGACAATAATTCTCGAGAGTTAGAGAGTAAGTATTCTATGTGAATAAAAGCTTCAGCTCCCCCCTCCCTTCTCAGTGGATGCAGGCCTTGCTTTCAGAATCACTTTAATTCTGTGTTGTGCATGAACTTCATCTCTTATCTCCCTAATTCCTTCTGATTG

Coding sequence (CDS)

ATGTTAAATGATTCAATAAAATCGTATAACATATACTGTTCCAAGTGCCAGAACTGTATCAACAATTTGATTCAAAGTGTTTTGAAATTAGAAGATGTTGAAGAATCATCTGCAAAATTTTCATACGAAGATGAAAAAGACATTCCAAAAAAGTGTTATGAAGTTCTTTATAAGTTTCTCAATTTGCATACCATACATTGTCCTGCTTGCACGAATTATGTCAGAGAGGTGACAATCCAACATGCTCCAAAACAATCCACGACTCTTGTACAAGTTCCAAAACCATCCATTCATGGTACAGATGAAAAAATTATAGACCATCAAGAAGGCTTAGTTATCACGATCTTCCATCCTGATGAACCAAGCACTAGAGTTAGAAGGTGGGAAATTCGTAGACTTATAGTACTTGGTGGTTTAATGGAATCAATTACAAGTTTAGCTATTGTAACTGCAGCAATGAGTGCAAAGATTTCTGATGGAAATATAGTAGCTTTGGCGTGGACAAATTTGATAGCTGGACTCTTTATTATTAGGCATGATGTATCAAGATTGCAGAAGAAGAGAAAAATACCTGGAATTGAAGTGGACGGCAATAACTACAAAGAAGTATTGAAGGAAAAAATGTATAGTCTTCTCTGTTTTATCATCGCCTACTTATCTTTCTTGTGTTTTGGGTTAGTGCCACCACTGGTTTATGCACTCTCATCCCTCAAGATCAGAAACAAAAACCTCAAAATCATAGCGGCTGCAGGAGCTTCCCTTTCATGCACTGCACTTCTTGCTGTCCAGAAAGCTCACATTAATCAAAAGCCAAGACATAGGCTAGTTTATGTCAAAACTGCAGCCGTAGATGTTTCAGTGGGAGTTGGGGCTTTTGGCATATCATATCTAGCAGGTTACATGTTTGGACACCTTGCATAG

Protein sequence

MLNDSIKSYNIYCSKCQNCINNLIQSVLKLEDVEESSAKFSYEDEKDIPKKCYEVLYKFLNLHTIHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTDEKIIDHQEGLVITIFHPDEPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRHDVSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAGYMFGHLA
Homology
BLAST of Carg11454 vs. NCBI nr
Match: KAG7014517.1 (Membrane protein of ER body 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 599.7 bits (1545), Expect = 1.4e-167
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 0

Query: 1   MLNDSIKSYNIYCSKCQNCINNLIQSVLKLEDVEESSAKFSYEDEKDIPKKCYEVLYKFL 60
           MLNDSIKSYNIYCSKCQNCINNLIQSVLKLEDVEESSAKFSYEDEKDIPKKCYEVLYKFL
Sbjct: 1   MLNDSIKSYNIYCSKCQNCINNLIQSVLKLEDVEESSAKFSYEDEKDIPKKCYEVLYKFL 60

Query: 61  NLHTIHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTDEKIIDHQEGLVITIFHPDE 120
           NLHTIHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTDEKIIDHQEGLVITIFHPDE
Sbjct: 61  NLHTIHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTDEKIIDHQEGLVITIFHPDE 120

Query: 121 PSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRHD 180
           PSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRHD
Sbjct: 121 PSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRHD 180

Query: 181 VSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIR 240
           VSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIR
Sbjct: 181 VSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIR 240

Query: 241 NKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAGY 300
           NKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAGY
Sbjct: 241 NKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAGY 300

Query: 301 MFGHLA 307
           MFGHLA
Sbjct: 301 MFGHLA 306

BLAST of Carg11454 vs. NCBI nr
Match: XP_022954207.1 (uncharacterized protein LOC111456533 isoform X2 [Cucurbita moschata])

HSP 1 Score: 584.3 bits (1505), Expect = 6.0e-163
Identity = 299/306 (97.71%), Postives = 302/306 (98.69%), Query Frame = 0

Query: 1   MLNDSIKSYNIYCSKCQNCINNLIQSVLKLEDVEESSAKFSYEDEKDIPKKCYEVLYKFL 60
           MLNDSIKSYNIYCSKCQNCINNLIQS LKLEDVEESSAKFSYEDEKDIPKKCYEV+YKFL
Sbjct: 598 MLNDSIKSYNIYCSKCQNCINNLIQSDLKLEDVEESSAKFSYEDEKDIPKKCYEVIYKFL 657

Query: 61  NLHTIHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTDEKIIDHQEGLVITIFHPDE 120
           NLHT HCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTDE+IIDHQEGLVITIFHPDE
Sbjct: 658 NLHTTHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTDEEIIDHQEGLVITIFHPDE 717

Query: 121 PSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRHD 180
           PSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNI+ALA TNLIAGLFIIRHD
Sbjct: 718 PSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRHD 777

Query: 181 VSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIR 240
           VSRLQKKRKIP IEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIR
Sbjct: 778 VSRLQKKRKIPRIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIR 837

Query: 241 NKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAGY 300
           NKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAGY
Sbjct: 838 NKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAGY 897

Query: 301 MFGHLA 307
           MFGHLA
Sbjct: 898 MFGHLA 903

BLAST of Carg11454 vs. NCBI nr
Match: KAG6575995.1 (Membrane protein of ER body 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 581.6 bits (1498), Expect = 3.9e-162
Identity = 300/306 (98.04%), Postives = 301/306 (98.37%), Query Frame = 0

Query: 1   MLNDSIKSYNIYCSKCQNCINNLIQSVLKLEDVEESSAKFSYEDEKDIPKKCYEVLYKFL 60
           MLNDSIKSYNIYCSKCQNCINNLIQS LKLEDVEESSAKFSYEDEKDIPKKCYEVLYKFL
Sbjct: 570 MLNDSIKSYNIYCSKCQNCINNLIQSDLKLEDVEESSAKFSYEDEKDIPKKCYEVLYKFL 629

Query: 61  NLHTIHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTDEKIIDHQEGLVITIFHPDE 120
           NLHTIHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTDEKIIDHQEGLVITIFHPDE
Sbjct: 630 NLHTIHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTDEKIIDHQEGLVITIFHPDE 689

Query: 121 PSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRHD 180
           P TRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNI+ALA TNLIAGLFIIRHD
Sbjct: 690 PRTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRHD 749

Query: 181 VSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIR 240
           VSRLQ KRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIR
Sbjct: 750 VSRLQ-KRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIR 809

Query: 241 NKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAGY 300
           NKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVS GVGAFGISYLAGY
Sbjct: 810 NKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSAGVGAFGISYLAGY 869

Query: 301 MFGHLA 307
           MFGHLA
Sbjct: 870 MFGHLA 874

BLAST of Carg11454 vs. NCBI nr
Match: XP_022954206.1 (uncharacterized protein LOC111456533 isoform X1 [Cucurbita moschata])

HSP 1 Score: 579.7 bits (1493), Expect = 1.5e-161
Identity = 299/307 (97.39%), Postives = 302/307 (98.37%), Query Frame = 0

Query: 1   MLNDSIKSYNIYCSKCQNCINNLIQSVLKLEDVEESSAKFSYEDEKDIPKKCYEVLYKFL 60
           MLNDSIKSYNIYCSKCQNCINNLIQS LKLEDVEESSAKFSYEDEKDIPKKCYEV+YKFL
Sbjct: 598 MLNDSIKSYNIYCSKCQNCINNLIQSDLKLEDVEESSAKFSYEDEKDIPKKCYEVIYKFL 657

Query: 61  NLHTIHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGT-DEKIIDHQEGLVITIFHPD 120
           NLHT HCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGT DE+IIDHQEGLVITIFHPD
Sbjct: 658 NLHTTHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTADEEIIDHQEGLVITIFHPD 717

Query: 121 EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRH 180
           EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNI+ALA TNLIAGLFIIRH
Sbjct: 718 EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRH 777

Query: 181 DVSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKI 240
           DVSRLQKKRKIP IEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKI
Sbjct: 778 DVSRLQKKRKIPRIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKI 837

Query: 241 RNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAG 300
           RNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAG
Sbjct: 838 RNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAG 897

Query: 301 YMFGHLA 307
           YMFGHLA
Sbjct: 898 YMFGHLA 904

BLAST of Carg11454 vs. NCBI nr
Match: XP_022954208.1 (uncharacterized protein LOC111456533 isoform X3 [Cucurbita moschata])

HSP 1 Score: 579.7 bits (1493), Expect = 1.5e-161
Identity = 299/307 (97.39%), Postives = 302/307 (98.37%), Query Frame = 0

Query: 1   MLNDSIKSYNIYCSKCQNCINNLIQSVLKLEDVEESSAKFSYEDEKDIPKKCYEVLYKFL 60
           MLNDSIKSYNIYCSKCQNCINNLIQS LKLEDVEESSAKFSYEDEKDIPKKCYEV+YKFL
Sbjct: 410 MLNDSIKSYNIYCSKCQNCINNLIQSDLKLEDVEESSAKFSYEDEKDIPKKCYEVIYKFL 469

Query: 61  NLHTIHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGT-DEKIIDHQEGLVITIFHPD 120
           NLHT HCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGT DE+IIDHQEGLVITIFHPD
Sbjct: 470 NLHTTHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTADEEIIDHQEGLVITIFHPD 529

Query: 121 EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRH 180
           EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNI+ALA TNLIAGLFIIRH
Sbjct: 530 EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRH 589

Query: 181 DVSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKI 240
           DVSRLQKKRKIP IEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKI
Sbjct: 590 DVSRLQKKRKIPRIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKI 649

Query: 241 RNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAG 300
           RNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAG
Sbjct: 650 RNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAG 709

Query: 301 YMFGHLA 307
           YMFGHLA
Sbjct: 710 YMFGHLA 716

BLAST of Carg11454 vs. ExPASy Swiss-Prot
Match: Q8LPT3 (Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.0e-19
Identity = 66/183 (36.07%), Postives = 101/183 (55.19%), Query Frame = 0

Query: 126 RRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRHDVSRLQ 185
           R+ EI + IV GGL+E+ITSL ++++A  +  S  NI+ L   NL+ GL +I H++  L+
Sbjct: 541 RKLEILKSIVYGGLLEAITSLGVISSAAGSGASMLNILVLGLANLLGGLILIIHNLQELR 600

Query: 186 KKRKI---------PGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSS 245
           ++  I          G E +   YK +L  +    L   +A LSF+  G++PP+VY  S 
Sbjct: 601 EEEPIRTTTEDNQTNGREEEEGRYKRLLGRRENFTLHATVAILSFIITGILPPVVYYFSF 660

Query: 246 LKIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISY 300
            +  NK+ K+ +  GASL C  LLA+ KAH+      R  Y+K+     S+ V   GISY
Sbjct: 661 SEKHNKDYKVASVFGASLFCIVLLAIAKAHVRYP---RGSYLKSILYYGSIAVSVSGISY 720

BLAST of Carg11454 vs. ExPASy Swiss-Prot
Match: Q8W4P8 (Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.1e-18
Identity = 72/193 (37.31%), Postives = 101/193 (52.33%), Query Frame = 0

Query: 120 EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRH 179
           EP     + EI + IV GGL ESITSL  VT+A ++  S  N++AL   NL +GL +  H
Sbjct: 403 EPRKGGNKVEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLLLTVH 462

Query: 180 DVSRL--QKKRKIPGI-------EVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPL 239
            +  L  +K RK           E + + Y+EVL  + YS +  +IA  SF+ FGL+PPL
Sbjct: 463 SLQELINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPL 522

Query: 240 VYALSSLKIRNK--NLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVG 299
           VY  S  K   K    K++A    SL C  LL++ KA++++K      YVKT     +  
Sbjct: 523 VYGFSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKKRD----YVKTLFRYTTTA 582

Query: 300 VGAFGISYLAGYM 302
             A G S   GY+
Sbjct: 583 TTASGFSQFVGYL 591

BLAST of Carg11454 vs. ExPASy Swiss-Prot
Match: F4KFS7 (Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.5e-18
Identity = 69/207 (33.33%), Postives = 113/207 (54.59%), Query Frame = 0

Query: 102 EKIIDHQEGLVITIFHPD-EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDG 161
           E+ ID ++ +  T   P  +P       E+ +  V GGL E+ITSL +V++A ++  S  
Sbjct: 317 EEYIDVRKSINTTQSPPQIQPDGERFAIELLKSTVYGGLTETITSLGVVSSASASGSSTM 376

Query: 162 NIVALAWTNLIAGLFIIRHDVSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLS 221
           NI+ALA  NL  GL ++  +   L+        + + + Y+E+L  +  S +  ++A +S
Sbjct: 377 NILALAVANLAGGLIVLAQNFQDLRNSS-----DQEKDRYEELLGRRTKSRIHILVAVMS 436

Query: 222 FLCFGLVPPLVYALSSLKIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRL----V 281
           ++ FGL+PPLVYA S  +   KN K+I+    SL C  LL   K ++ +KP +       
Sbjct: 437 YIFFGLIPPLVYAFSFYETGIKNYKLISVFLGSLVCVILLGSIKVYV-RKPTNSCGSTKA 496

Query: 282 YVKTAAVDVSVGVGAFGISYLAGYMFG 304
           Y+K+AA   S+ V + GISY+ G + G
Sbjct: 497 YLKSAAYYTSIVVASCGISYVVGDIMG 517

BLAST of Carg11454 vs. ExPASy TrEMBL
Match: A0A6J1GQF8 (uncharacterized protein LOC111456533 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456533 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 2.9e-163
Identity = 299/306 (97.71%), Postives = 302/306 (98.69%), Query Frame = 0

Query: 1   MLNDSIKSYNIYCSKCQNCINNLIQSVLKLEDVEESSAKFSYEDEKDIPKKCYEVLYKFL 60
           MLNDSIKSYNIYCSKCQNCINNLIQS LKLEDVEESSAKFSYEDEKDIPKKCYEV+YKFL
Sbjct: 598 MLNDSIKSYNIYCSKCQNCINNLIQSDLKLEDVEESSAKFSYEDEKDIPKKCYEVIYKFL 657

Query: 61  NLHTIHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTDEKIIDHQEGLVITIFHPDE 120
           NLHT HCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTDE+IIDHQEGLVITIFHPDE
Sbjct: 658 NLHTTHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTDEEIIDHQEGLVITIFHPDE 717

Query: 121 PSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRHD 180
           PSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNI+ALA TNLIAGLFIIRHD
Sbjct: 718 PSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRHD 777

Query: 181 VSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIR 240
           VSRLQKKRKIP IEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIR
Sbjct: 778 VSRLQKKRKIPRIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIR 837

Query: 241 NKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAGY 300
           NKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAGY
Sbjct: 838 NKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAGY 897

Query: 301 MFGHLA 307
           MFGHLA
Sbjct: 898 MFGHLA 903

BLAST of Carg11454 vs. ExPASy TrEMBL
Match: A0A6J1GQA4 (uncharacterized protein LOC111456533 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456533 PE=3 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.1e-162
Identity = 299/307 (97.39%), Postives = 302/307 (98.37%), Query Frame = 0

Query: 1   MLNDSIKSYNIYCSKCQNCINNLIQSVLKLEDVEESSAKFSYEDEKDIPKKCYEVLYKFL 60
           MLNDSIKSYNIYCSKCQNCINNLIQS LKLEDVEESSAKFSYEDEKDIPKKCYEV+YKFL
Sbjct: 598 MLNDSIKSYNIYCSKCQNCINNLIQSDLKLEDVEESSAKFSYEDEKDIPKKCYEVIYKFL 657

Query: 61  NLHTIHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGT-DEKIIDHQEGLVITIFHPD 120
           NLHT HCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGT DE+IIDHQEGLVITIFHPD
Sbjct: 658 NLHTTHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTADEEIIDHQEGLVITIFHPD 717

Query: 121 EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRH 180
           EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNI+ALA TNLIAGLFIIRH
Sbjct: 718 EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRH 777

Query: 181 DVSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKI 240
           DVSRLQKKRKIP IEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKI
Sbjct: 778 DVSRLQKKRKIPRIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKI 837

Query: 241 RNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAG 300
           RNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAG
Sbjct: 838 RNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAG 897

Query: 301 YMFGHLA 307
           YMFGHLA
Sbjct: 898 YMFGHLA 904

BLAST of Carg11454 vs. ExPASy TrEMBL
Match: A0A6J1GQF7 (uncharacterized protein LOC111456533 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111456533 PE=3 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.1e-162
Identity = 299/307 (97.39%), Postives = 302/307 (98.37%), Query Frame = 0

Query: 1   MLNDSIKSYNIYCSKCQNCINNLIQSVLKLEDVEESSAKFSYEDEKDIPKKCYEVLYKFL 60
           MLNDSIKSYNIYCSKCQNCINNLIQS LKLEDVEESSAKFSYEDEKDIPKKCYEV+YKFL
Sbjct: 410 MLNDSIKSYNIYCSKCQNCINNLIQSDLKLEDVEESSAKFSYEDEKDIPKKCYEVIYKFL 469

Query: 61  NLHTIHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGT-DEKIIDHQEGLVITIFHPD 120
           NLHT HCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGT DE+IIDHQEGLVITIFHPD
Sbjct: 470 NLHTTHCPACTNYVREVTIQHAPKQSTTLVQVPKPSIHGTADEEIIDHQEGLVITIFHPD 529

Query: 121 EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRH 180
           EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNI+ALA TNLIAGLFIIRH
Sbjct: 530 EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIIALALTNLIAGLFIIRH 589

Query: 181 DVSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKI 240
           DVSRLQKKRKIP IEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKI
Sbjct: 590 DVSRLQKKRKIPRIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKI 649

Query: 241 RNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAG 300
           RNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAG
Sbjct: 650 RNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAG 709

Query: 301 YMFGHLA 307
           YMFGHLA
Sbjct: 710 YMFGHLA 716

BLAST of Carg11454 vs. ExPASy TrEMBL
Match: A0A6J1JQG4 (membrane protein of ER body 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488012 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 2.4e-85
Identity = 180/200 (90.00%), Postives = 184/200 (92.00%), Query Frame = 0

Query: 107 HQEGLVITIFHPDEPSTRV-RRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVAL 166
           HQE L I I  P EPSTRV RRWEIRR IVLGGLMESITSLAIVTAAM+AKISDGNIVAL
Sbjct: 3   HQECLAIMI-PPYEPSTRVRRRWEIRRYIVLGGLMESITSLAIVTAAMTAKISDGNIVAL 62

Query: 167 AWTNLIAGLFIIRHDVSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFG 226
           A TNL+AGLF+IRHDVSRLQKKRKIP IEVDGNNYKEVLKEKMY LLCFIIAYLSFL FG
Sbjct: 63  ALTNLLAGLFVIRHDVSRLQKKRKIPRIEVDGNNYKEVLKEKMYRLLCFIIAYLSFLFFG 122

Query: 227 LVPPLVYALSSLKIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDV 286
           LVPPLVYALSSLKIRNKNLKII AAGASLSCTALLAVQKAHINQKPR+RLVYVKTAAVDV
Sbjct: 123 LVPPLVYALSSLKIRNKNLKIIVAAGASLSCTALLAVQKAHINQKPRNRLVYVKTAAVDV 182

Query: 287 SVGVGAFGISYLAGYMFGHL 306
           SV VGAFGISYL GYMFG L
Sbjct: 183 SVRVGAFGISYLVGYMFGTL 201

BLAST of Carg11454 vs. ExPASy TrEMBL
Match: A0A5D3CME8 (Membrane protein of ER body-like protein isoform X4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold263G00400 PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 4.0e-32
Identity = 98/233 (42.06%), Postives = 136/233 (58.37%), Query Frame = 0

Query: 76  EVTIQHAPKQSTTLVQVPKPSIHGTDEKIIDHQEGLVITIFHP-----DEPSTRVRRWEI 135
           E T+  +   S +L + P      TD  +  H+  + I+I        +  S+R  RWEI
Sbjct: 531 EATVPDSSPVSASL-EAPTTVERATDTAVGSHEGPVAISIDDSLDEQIEPESSRYNRWEI 590

Query: 136 RRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRHDVSRLQKKRKI 195
            + IV GGL ESITSL IVT+A SA    GNIV L+  NLI+GLFI+ H+++ L+ ++  
Sbjct: 591 VKSIVYGGLAESITSLGIVTSAASANTGTGNIVVLSLANLISGLFILGHNLTGLKSEQFR 650

Query: 196 PGIEVDGNN----YKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSSLKIRNKNLKI 255
              E D ++    Y+ VL  +   +L F++A  SF+ FGLVPPLVY  S  K  +K+LK+
Sbjct: 651 TSNETDDDDHVDRYEVVLGNRENYILHFVLAIFSFVLFGLVPPLVYGFSFTKSNDKDLKL 710

Query: 256 IAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISYLAG 300
            A AGASL C  LLA+ KA+I Q+P    VY+KT    + +  GA G SYLAG
Sbjct: 711 TAVAGASLLCITLLALGKAYI-QRPNRWDVYIKTVVSYIVIAAGAGGFSYLAG 761

BLAST of Carg11454 vs. TAIR 10
Match: AT4G27870.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 99.0 bits (245), Expect = 7.1e-21
Identity = 66/183 (36.07%), Postives = 101/183 (55.19%), Query Frame = 0

Query: 126 RRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRHDVSRLQ 185
           R+ EI + IV GGL+E+ITSL ++++A  +  S  NI+ L   NL+ GL +I H++  L+
Sbjct: 541 RKLEILKSIVYGGLLEAITSLGVISSAAGSGASMLNILVLGLANLLGGLILIIHNLQELR 600

Query: 186 KKRKI---------PGIEVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSS 245
           ++  I          G E +   YK +L  +    L   +A LSF+  G++PP+VY  S 
Sbjct: 601 EEEPIRTTTEDNQTNGREEEEGRYKRLLGRRENFTLHATVAILSFIITGILPPVVYYFSF 660

Query: 246 LKIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGISY 300
            +  NK+ K+ +  GASL C  LLA+ KAH+      R  Y+K+     S+ V   GISY
Sbjct: 661 SEKHNKDYKVASVFGASLFCIVLLAIAKAHVRYP---RGSYLKSILYYGSIAVSVSGISY 720

BLAST of Carg11454 vs. TAIR 10
Match: AT4G27860.1 (vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 95.5 bits (236), Expect = 7.8e-20
Identity = 72/193 (37.31%), Postives = 101/193 (52.33%), Query Frame = 0

Query: 120 EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRH 179
           EP     + EI + IV GGL ESITSL  VT+A ++  S  N++AL   NL +GL +  H
Sbjct: 403 EPRKGGNKVEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLLLTVH 462

Query: 180 DVSRL--QKKRKIPGI-------EVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPL 239
            +  L  +K RK           E + + Y+EVL  + YS +  +IA  SF+ FGL+PPL
Sbjct: 463 SLQELINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPL 522

Query: 240 VYALSSLKIRNK--NLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVG 299
           VY  S  K   K    K++A    SL C  LL++ KA++++K      YVKT     +  
Sbjct: 523 VYGFSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKKRD----YVKTLFRYTTTA 582

Query: 300 VGAFGISYLAGYM 302
             A G S   GY+
Sbjct: 583 TTASGFSQFVGYL 591

BLAST of Carg11454 vs. TAIR 10
Match: AT4G27860.2 (vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 95.5 bits (236), Expect = 7.8e-20
Identity = 72/193 (37.31%), Postives = 101/193 (52.33%), Query Frame = 0

Query: 120 EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDGNIVALAWTNLIAGLFIIRH 179
           EP     + EI + IV GGL ESITSL  VT+A ++  S  N++AL   NL +GL +  H
Sbjct: 388 EPRKGGNKVEILKSIVYGGLTESITSLCTVTSAAASGASTLNVLALGVANLSSGLLLTVH 447

Query: 180 DVSRL--QKKRKIPGI-------EVDGNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPL 239
            +  L  +K RK           E + + Y+EVL  + YS +  +IA  SF+ FGL+PPL
Sbjct: 448 SLQELINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPL 507

Query: 240 VYALSSLKIRNK--NLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVG 299
           VY  S  K   K    K++A    SL C  LL++ KA++++K      YVKT     +  
Sbjct: 508 VYGFSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKKRD----YVKTLFRYTTTA 567

Query: 300 VGAFGISYLAGYM 302
             A G S   GY+
Sbjct: 568 TTASGFSQFVGYL 576

BLAST of Carg11454 vs. TAIR 10
Match: AT5G24290.2 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.7e-19
Identity = 69/207 (33.33%), Postives = 113/207 (54.59%), Query Frame = 0

Query: 102 EKIIDHQEGLVITIFHPD-EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDG 161
           E+ ID ++ +  T   P  +P       E+ +  V GGL E+ITSL +V++A ++  S  
Sbjct: 301 EEYIDVRKSINTTQSPPQIQPDGERFAIELLKSTVYGGLTETITSLGVVSSASASGSSTM 360

Query: 162 NIVALAWTNLIAGLFIIRHDVSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLS 221
           NI+ALA  NL  GL ++  +   L+        + + + Y+E+L  +  S +  ++A +S
Sbjct: 361 NILALAVANLAGGLIVLAQNFQDLRNSS-----DQEKDRYEELLGRRTKSRIHILVAVMS 420

Query: 222 FLCFGLVPPLVYALSSLKIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRL----V 281
           ++ FGL+PPLVYA S  +   KN K+I+    SL C  LL   K ++ +KP +       
Sbjct: 421 YIFFGLIPPLVYAFSFYETGIKNYKLISVFLGSLVCVILLGSIKVYV-RKPTNSCGSTKA 480

Query: 282 YVKTAAVDVSVGVGAFGISYLAGYMFG 304
           Y+K+AA   S+ V + GISY+ G + G
Sbjct: 481 YLKSAAYYTSIVVASCGISYVVGDIMG 501

BLAST of Carg11454 vs. TAIR 10
Match: AT5G24290.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.7e-19
Identity = 69/207 (33.33%), Postives = 113/207 (54.59%), Query Frame = 0

Query: 102 EKIIDHQEGLVITIFHPD-EPSTRVRRWEIRRLIVLGGLMESITSLAIVTAAMSAKISDG 161
           E+ ID ++ +  T   P  +P       E+ +  V GGL E+ITSL +V++A ++  S  
Sbjct: 317 EEYIDVRKSINTTQSPPQIQPDGERFAIELLKSTVYGGLTETITSLGVVSSASASGSSTM 376

Query: 162 NIVALAWTNLIAGLFIIRHDVSRLQKKRKIPGIEVDGNNYKEVLKEKMYSLLCFIIAYLS 221
           NI+ALA  NL  GL ++  +   L+        + + + Y+E+L  +  S +  ++A +S
Sbjct: 377 NILALAVANLAGGLIVLAQNFQDLRNSS-----DQEKDRYEELLGRRTKSRIHILVAVMS 436

Query: 222 FLCFGLVPPLVYALSSLKIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRL----V 281
           ++ FGL+PPLVYA S  +   KN K+I+    SL C  LL   K ++ +KP +       
Sbjct: 437 YIFFGLIPPLVYAFSFYETGIKNYKLISVFLGSLVCVILLGSIKVYV-RKPTNSCGSTKA 496

Query: 282 YVKTAAVDVSVGVGAFGISYLAGYMFG 304
           Y+K+AA   S+ V + GISY+ G + G
Sbjct: 497 YLKSAAYYTSIVVASCGISYVVGDIMG 517

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014517.11.4e-167100.00Membrane protein of ER body 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022954207.16.0e-16397.71uncharacterized protein LOC111456533 isoform X2 [Cucurbita moschata][more]
KAG6575995.13.9e-16298.04Membrane protein of ER body 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022954206.11.5e-16197.39uncharacterized protein LOC111456533 isoform X1 [Cucurbita moschata][more]
XP_022954208.11.5e-16197.39uncharacterized protein LOC111456533 isoform X3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8LPT31.0e-1936.07Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL... [more]
Q8W4P81.1e-1837.31Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1[more]
F4KFS72.5e-1833.33Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GQF82.9e-16397.71uncharacterized protein LOC111456533 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1GQA47.1e-16297.39uncharacterized protein LOC111456533 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1GQF77.1e-16297.39uncharacterized protein LOC111456533 isoform X3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JQG42.4e-8590.00membrane protein of ER body 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488012 P... [more]
A0A5D3CME84.0e-3242.06Membrane protein of ER body-like protein isoform X4 OS=Cucumis melo var. makuwa ... [more]
Match NameE-valueIdentityDescription
AT4G27870.17.1e-2136.07Vacuolar iron transporter (VIT) family protein [more]
AT4G27860.17.8e-2037.31vacuolar iron transporter (VIT) family protein [more]
AT4G27860.27.8e-2037.31vacuolar iron transporter (VIT) family protein [more]
AT5G24290.21.7e-1933.33Vacuolar iron transporter (VIT) family protein [more]
AT5G24290.11.7e-1933.33Vacuolar iron transporter (VIT) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR31851:SF64GLYCINE-RICH PROTEIN FAMILYcoord: 96..303
IPR008217Ccc1 familyPANTHERPTHR31851FE(2+)/MN(2+) TRANSPORTER PCL1coord: 96..303

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg11454-RACarg11454-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030026 cellular manganese ion homeostasis
biological_process GO:0071421 manganese ion transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005384 manganese ion transmembrane transporter activity